"The isolation and estimation of low molecular weight N-nitrosamines in biological materials"
- Authors: Du Plessis, Leo Stephen
- Date: 1973
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:21176 , http://hdl.handle.net/10962/6750
- Description: Summary: Low molecular weight N-nitrosamines were detected by ultraviolet, infrared, nuclear magnetic resonance, mass spectral, thin-layer chromatographic and gas liquid chromatographic means. A method for the estimation of dimethylnitrosamine, diethylnitrosamine, ethal-n-propylnitrosamine and di-n-propylnitrosamine has been developed. The method involves the isolation of the nitrosamines in an aqueous distillate by freeze-drying. After extraction of the nitrosamines from the aqueous distillate by means of dichloromethane, their separation and quantitative estimation are achieved by gas liquid chromatography of the extract. The procedure can be extended down to the ppb range. Dimethylnitrosamine was isolated from an extract of Solanum incanum and identified by gas liquid chromatography on four different columns, infrared and nuclear magnetic resonance spectroscopy.
- Full Text:
- Date Issued: 1973
- Authors: Du Plessis, Leo Stephen
- Date: 1973
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:21176 , http://hdl.handle.net/10962/6750
- Description: Summary: Low molecular weight N-nitrosamines were detected by ultraviolet, infrared, nuclear magnetic resonance, mass spectral, thin-layer chromatographic and gas liquid chromatographic means. A method for the estimation of dimethylnitrosamine, diethylnitrosamine, ethal-n-propylnitrosamine and di-n-propylnitrosamine has been developed. The method involves the isolation of the nitrosamines in an aqueous distillate by freeze-drying. After extraction of the nitrosamines from the aqueous distillate by means of dichloromethane, their separation and quantitative estimation are achieved by gas liquid chromatography of the extract. The procedure can be extended down to the ppb range. Dimethylnitrosamine was isolated from an extract of Solanum incanum and identified by gas liquid chromatography on four different columns, infrared and nuclear magnetic resonance spectroscopy.
- Full Text:
- Date Issued: 1973
A physico-chemical investigation of refractory hard metals
- Authors: Philip, Hamish Ian
- Date: 1973
- Subjects: Refractory materials Heat resistant alloys
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4441 , http://hdl.handle.net/10962/d1007630
- Description: In this investigation, the resistivity of vanadium nitride at elevated temperatures has been measured using a resistance bridge based on the Dauphinée/Mooser (1955) chopper system. In general, an almost linear increase of resistivity with temperature was observed for VN, but at certain temperatures, deviations from linearity (anomalies) were observed, these have been classified into three types. Attempts have been made to correlate the anomalous behaviour and temperature coefficients of resistivity with the ambient atmospheres and with the impurities, (especially oxygen), stoichiotmetry and density of this material. Oxygen and excess nitrogen appear to be electron donors in their effects on VN and result in a decrease in the slope of resistivity vs temperature curves. Oxygen and excess nitrogen also appear to increase the overall resistivity due to an increase in defect concentration. No effects due to the ambient atmosphere have been observed in the case of hot-pressed samples. lndicating that reaction can only occur if the reacting gases can reach the interior of the samples.
- Full Text:
- Date Issued: 1973
- Authors: Philip, Hamish Ian
- Date: 1973
- Subjects: Refractory materials Heat resistant alloys
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4441 , http://hdl.handle.net/10962/d1007630
- Description: In this investigation, the resistivity of vanadium nitride at elevated temperatures has been measured using a resistance bridge based on the Dauphinée/Mooser (1955) chopper system. In general, an almost linear increase of resistivity with temperature was observed for VN, but at certain temperatures, deviations from linearity (anomalies) were observed, these have been classified into three types. Attempts have been made to correlate the anomalous behaviour and temperature coefficients of resistivity with the ambient atmospheres and with the impurities, (especially oxygen), stoichiotmetry and density of this material. Oxygen and excess nitrogen appear to be electron donors in their effects on VN and result in a decrease in the slope of resistivity vs temperature curves. Oxygen and excess nitrogen also appear to increase the overall resistivity due to an increase in defect concentration. No effects due to the ambient atmosphere have been observed in the case of hot-pressed samples. lndicating that reaction can only occur if the reacting gases can reach the interior of the samples.
- Full Text:
- Date Issued: 1973
A structural investigation of the sulphated polysaccharide from Aeodes ulvoidea Schmitz
- Allsobrook, Anthony John Robert
- Authors: Allsobrook, Anthony John Robert
- Date: 1973
- Subjects: Red algae -- Composition Polysaccharides
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4437 , http://hdl.handle.net/10962/d1007482
- Description: Aeodes ulvoidea, a red seaweed of the Grateloupiaceae, yielded a highly sulphated polysaccharide which was shown to contain D- and L-galactose, 4-0-methy-L-galactose, 2-0-methyl - D- and L-galactose and 6-0-methyl-D-galactose, together with chromatographic traces of xylose and mannose. The sulphate was not labile to alkali, but it was largely removed with methanolic hydrogen chloride. Periodate oxidation of the polysaccharide, methylation of the de sulphated polysaccharide, and investigation of fifteen oligosaccharides from partial hydrolysis and acetolysis studies of the polysaccharide, indicate that (a) the polysaccharide is composed of a backbone of D-galactose residues which are 1,3- and 1,4-linked (b) at least some regions of alternating structure do occur (c) the 2-0-methylgalactose is linked through the 4-position (d) the 4-0-methyl-L-galactose is present as single unit side chains glycosidically linked to the galactose backbone at position 6, and (e) most of the 6-0-methyl-D-galactose is linked to the 4-position of 2-0-methyl-D-galactose.
- Full Text:
- Date Issued: 1973
- Authors: Allsobrook, Anthony John Robert
- Date: 1973
- Subjects: Red algae -- Composition Polysaccharides
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4437 , http://hdl.handle.net/10962/d1007482
- Description: Aeodes ulvoidea, a red seaweed of the Grateloupiaceae, yielded a highly sulphated polysaccharide which was shown to contain D- and L-galactose, 4-0-methy-L-galactose, 2-0-methyl - D- and L-galactose and 6-0-methyl-D-galactose, together with chromatographic traces of xylose and mannose. The sulphate was not labile to alkali, but it was largely removed with methanolic hydrogen chloride. Periodate oxidation of the polysaccharide, methylation of the de sulphated polysaccharide, and investigation of fifteen oligosaccharides from partial hydrolysis and acetolysis studies of the polysaccharide, indicate that (a) the polysaccharide is composed of a backbone of D-galactose residues which are 1,3- and 1,4-linked (b) at least some regions of alternating structure do occur (c) the 2-0-methylgalactose is linked through the 4-position (d) the 4-0-methyl-L-galactose is present as single unit side chains glycosidically linked to the galactose backbone at position 6, and (e) most of the 6-0-methyl-D-galactose is linked to the 4-position of 2-0-methyl-D-galactose.
- Full Text:
- Date Issued: 1973
Metal complexes of black wattle tannins and related model polyphenols
- Authors: Slabbert, Neville Patrick
- Date: 1973
- Subjects: Metal complexes Tannins
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4432 , http://hdl.handle.net/10962/d1007407
- Description: Apart from their general usage as tanning agents for hides and skins, the natural tannins have been used since earliest times for various other purposes. The blue-black iron tannate complex was used by ancient Egyptians as a hairdye and for many conturies this complex was the main source of writing inks. Wattle tannin is known to form complexes with many metal ions. The chief use of tannin complexes has been in gravimetric analyses, since aqueous solutions of tannins readily precipitate metal ions under certain experimentel conditions. At the present time the nature of the precipitated complexes has not been investigated however, because in the gravimetric method the complexes are ignited and the metal determined as its oxide; hence no knowledge of the complexes themselves was required.
- Full Text:
- Date Issued: 1973
- Authors: Slabbert, Neville Patrick
- Date: 1973
- Subjects: Metal complexes Tannins
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4432 , http://hdl.handle.net/10962/d1007407
- Description: Apart from their general usage as tanning agents for hides and skins, the natural tannins have been used since earliest times for various other purposes. The blue-black iron tannate complex was used by ancient Egyptians as a hairdye and for many conturies this complex was the main source of writing inks. Wattle tannin is known to form complexes with many metal ions. The chief use of tannin complexes has been in gravimetric analyses, since aqueous solutions of tannins readily precipitate metal ions under certain experimentel conditions. At the present time the nature of the precipitated complexes has not been investigated however, because in the gravimetric method the complexes are ignited and the metal determined as its oxide; hence no knowledge of the complexes themselves was required.
- Full Text:
- Date Issued: 1973
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