Evaluation for harnessing low-enthalpy geothermal energy in South Africa based on a model pilot plant in the Limpopo Mobile Belt
- Authors: Dhansay, Taufeeq
- Date: 2012
- Subjects: Geothermal resources -- South Africa -- Limpopo , Renewable energy sources -- South Africa -- Limpopo , Climatic changes -- South Africa -- Limpopo
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10667 , http://hdl.handle.net/10948/d1019789
- Description: South Africa generates more than 90 percent of its total energy capacity through non-renewable sources. With coal forming the predominant energy source, South Africa became the leading carbon emissive nation in Africa, emitting 450 million tonnes of CO2 in 2011. In an international effort to restrict global average temperature rise to 2° C above the average prior the industrial revolution, the Kyoto Protocol has been extended for another 8-year commitment period. This is complementary to an expected resolution of a new legally binding climate change policy in 2015. This policy will aim to introduce financial penalties for nations failing to meet ascribed GHG emission targets by 2020. In an attempt to meet these climate change resolutions South Africa will research and develop cleaner, alternative forms of energy, including hydro, wind, and biomass forms of renewable energy, in addition to designating stringent building regulations for the Incorporation of solar energy. These measures form part of an Integrated evelopment Plan that aims to generate a target of 10,000 GWh of renewable energy in 2013. South Africa is also investigating the possibilities of extracting its shale gas reserves and implementing it as a major energy source. This energy mix has given little attention to geothermal energy. The reasons for this omission appears to be the lack of active volcanism and previous research that suggests South Africa, largely underlain by the Kaapvaal Craton, has a relatively low heat Flow profile, deemed insufficient for harnessing geothermal energy.
- Full Text:
- Date Issued: 2012
- Authors: Dhansay, Taufeeq
- Date: 2012
- Subjects: Geothermal resources -- South Africa -- Limpopo , Renewable energy sources -- South Africa -- Limpopo , Climatic changes -- South Africa -- Limpopo
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10667 , http://hdl.handle.net/10948/d1019789
- Description: South Africa generates more than 90 percent of its total energy capacity through non-renewable sources. With coal forming the predominant energy source, South Africa became the leading carbon emissive nation in Africa, emitting 450 million tonnes of CO2 in 2011. In an international effort to restrict global average temperature rise to 2° C above the average prior the industrial revolution, the Kyoto Protocol has been extended for another 8-year commitment period. This is complementary to an expected resolution of a new legally binding climate change policy in 2015. This policy will aim to introduce financial penalties for nations failing to meet ascribed GHG emission targets by 2020. In an attempt to meet these climate change resolutions South Africa will research and develop cleaner, alternative forms of energy, including hydro, wind, and biomass forms of renewable energy, in addition to designating stringent building regulations for the Incorporation of solar energy. These measures form part of an Integrated evelopment Plan that aims to generate a target of 10,000 GWh of renewable energy in 2013. South Africa is also investigating the possibilities of extracting its shale gas reserves and implementing it as a major energy source. This energy mix has given little attention to geothermal energy. The reasons for this omission appears to be the lack of active volcanism and previous research that suggests South Africa, largely underlain by the Kaapvaal Craton, has a relatively low heat Flow profile, deemed insufficient for harnessing geothermal energy.
- Full Text:
- Date Issued: 2012
Evaluation of noise levels of micro-wind turbines using a randomised experiment
- Authors: Clohessy, Chantelle May
- Date: 2012
- Subjects: Wind turbines -- Noise , Wind turbines -- valuation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10508 , http://hdl.handle.net/10948/d1015974
- Description: One of the biggest environmental concerns of a wind turbine is the wind turbine noise (Prospathopoulos and Voutsinas, 2007). This study assesses the noise impacts of wind turbines on the environment by comparing the micro-wind turbine noise to traditional accepted surrounding sounds. The collection of the sound level data was done by using a randomised experiment. The sound level data was then fitted to a General Linear Model to determine the relationship between the sound levels generated at a given site to the time of day, wind speed, wind direction and distance from the sound source. An additional study was conducted to determine the relationship between wind speed and the sound levels of wind turbines. The distribution of frequency components of wind turbine sound was also determined.
- Full Text:
- Date Issued: 2012
- Authors: Clohessy, Chantelle May
- Date: 2012
- Subjects: Wind turbines -- Noise , Wind turbines -- valuation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10508 , http://hdl.handle.net/10948/d1015974
- Description: One of the biggest environmental concerns of a wind turbine is the wind turbine noise (Prospathopoulos and Voutsinas, 2007). This study assesses the noise impacts of wind turbines on the environment by comparing the micro-wind turbine noise to traditional accepted surrounding sounds. The collection of the sound level data was done by using a randomised experiment. The sound level data was then fitted to a General Linear Model to determine the relationship between the sound levels generated at a given site to the time of day, wind speed, wind direction and distance from the sound source. An additional study was conducted to determine the relationship between wind speed and the sound levels of wind turbines. The distribution of frequency components of wind turbine sound was also determined.
- Full Text:
- Date Issued: 2012
Factors affecting survivorship of loggerhead (Caretta caretta) and leatherback (Dermochelys coriacea) sea turtles of South Africa
- Authors: De Wet, Anje
- Date: 2012
- Subjects: Loggerhead turtle -- South Africa , Leatherback turtle -- South Africa , Sea turtles -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10686 , http://hdl.handle.net/10948/d1007900 , Loggerhead turtle -- South Africa , Leatherback turtle -- South Africa , Sea turtles -- South Africa
- Description: Loggerhead (Caretta caretta) and leatherback (Dermochelys coriacea) sea turtles as well as their eggs and hatchlings have been protected on their nesting beach in South Africa (SA) since 1963. Both nesting populations were expected to show similar trends in recovery following the application of identical protection and conservation measures. The loggerhead nesting population has responded favourably to these protection efforts. In contrast, the leatherback nesting population showed an initial increase but is currently stable. The reason for this difference in response is thought to be due to differential offshore mortality of these two species. This prompted an investigation into the different sources of sea turtle mortality in the South Western Indian Ocean (SWIO). Specific aims were to identify and quantify sources of loggerhead and leatherback mortality on nesting beaches as well as in the oceans. Reasonable survivorship at all age classes is important to ensure recruitment of new nesting individuals into sea turtle populations. Mortality of nests, eggs per nest and hatchlings were quantified over two seasons for the loggerheads and leatherbacks nesting in SA. The beach was patrolled on foot to encounter and record females emerging from the ocean and later, hatchlings from their nests. The nests were then monitored during the incubation period and excavated once hatched. The fates of 925 nests were determined during these two nesting seasons (2009/2010 and 2010/2011). The main source of loggerhead and leatherback nest destruction was predation (8.6 percent and 15.7 percent respectively) followed by nest erosion (2.2 percent and 6.3 percent respectively). Overall nest success was high but higher for loggerheads (89 percent) than for leatherbacks (78 percent). The main cause of egg mortality for both species was early developmental arrest, followed by predation by ants and ghost crabs. Hatchlings en route to the sea were almost exclusively predated by ghost crabs (4.2 percent of emerged loggerhead hatchlings and 3.2 percent of emerged leatherback hatchlings). It appears that both species benefit from the coastal conservation efforts. When sea turtles leave the nesting beach, either as hatchlings or adults, conservation and monitoring becomes more difficult and sea turtles are exposed to a multitude of threats, including anthropogenic threats. Age classes tend to be spatially separated due to different habitat and dietary requirements. The type of threat sea turtles are exposed to thus depends on the current age class. Offshore sources of mortality in the SWIO were identified and where possible loggerhead and leatherback mortality was quantified and mapped spatially. Loggerheads were mostly exposed to and had the highest mortality in the artisanal fisheries in the SWIO (> 1000 per annum), inshore trawling (ca. 41 per annum), shark nets (protective gill nets) (21.6 ± 6.7 per annum) and the pelagic longline fishery (5.0 ± 4.4 per annum). In contrast, leatherbacks with a pelagic lifestyle, were mostly exposed to pelagic longline fisheries (7.8 ± 7.8 per annum). A spatial analysis of fishing activities indicated that leatherback home ranges overlapped 41percent with pelagic longline fishing activity in the SA EEZ, whereas the overlap between pelagic longliners and loggerhead home ranges was 29 percent. The quantified sources of mortality provide some explanation for the trend in the loggerhead nesting population but not the trend in the leatherback nesting population. Hatchling survivorship to adulthood was estimated to determine the viability of the two nesting populations as well as to determine whether offshore mortality was responsible for the difference in recovery of the two populations. Loggerhead hatchling survivorship to adulthood was estimated at between 2 and 10 per 1000 hatchlings, the minimum requirement for an increasing population. The adopted sophisticated model shows that leatherbacks have a survival rate of 5 to 10 per 1 000 hatchlings. However, this suggests that the population is increasing, but the leatherback population is stable. Perhaps the age to maturity of SA leatherbacks is greater than 12 years, or fisheries-related mortality affects younger age classes than initially thought. It is therefore recommended that the turtle monitoring area is extended to include other potential nesting grounds. In addition, observer or monitoring programs for commercial as well as artisanal fisheries needs to be extended throughout the SWIO to quantify sea turtle mortality. Ultimately a comprehensive multi-regional approach is required for the conservation of these highly migratory species.
- Full Text:
- Date Issued: 2012
- Authors: De Wet, Anje
- Date: 2012
- Subjects: Loggerhead turtle -- South Africa , Leatherback turtle -- South Africa , Sea turtles -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10686 , http://hdl.handle.net/10948/d1007900 , Loggerhead turtle -- South Africa , Leatherback turtle -- South Africa , Sea turtles -- South Africa
- Description: Loggerhead (Caretta caretta) and leatherback (Dermochelys coriacea) sea turtles as well as their eggs and hatchlings have been protected on their nesting beach in South Africa (SA) since 1963. Both nesting populations were expected to show similar trends in recovery following the application of identical protection and conservation measures. The loggerhead nesting population has responded favourably to these protection efforts. In contrast, the leatherback nesting population showed an initial increase but is currently stable. The reason for this difference in response is thought to be due to differential offshore mortality of these two species. This prompted an investigation into the different sources of sea turtle mortality in the South Western Indian Ocean (SWIO). Specific aims were to identify and quantify sources of loggerhead and leatherback mortality on nesting beaches as well as in the oceans. Reasonable survivorship at all age classes is important to ensure recruitment of new nesting individuals into sea turtle populations. Mortality of nests, eggs per nest and hatchlings were quantified over two seasons for the loggerheads and leatherbacks nesting in SA. The beach was patrolled on foot to encounter and record females emerging from the ocean and later, hatchlings from their nests. The nests were then monitored during the incubation period and excavated once hatched. The fates of 925 nests were determined during these two nesting seasons (2009/2010 and 2010/2011). The main source of loggerhead and leatherback nest destruction was predation (8.6 percent and 15.7 percent respectively) followed by nest erosion (2.2 percent and 6.3 percent respectively). Overall nest success was high but higher for loggerheads (89 percent) than for leatherbacks (78 percent). The main cause of egg mortality for both species was early developmental arrest, followed by predation by ants and ghost crabs. Hatchlings en route to the sea were almost exclusively predated by ghost crabs (4.2 percent of emerged loggerhead hatchlings and 3.2 percent of emerged leatherback hatchlings). It appears that both species benefit from the coastal conservation efforts. When sea turtles leave the nesting beach, either as hatchlings or adults, conservation and monitoring becomes more difficult and sea turtles are exposed to a multitude of threats, including anthropogenic threats. Age classes tend to be spatially separated due to different habitat and dietary requirements. The type of threat sea turtles are exposed to thus depends on the current age class. Offshore sources of mortality in the SWIO were identified and where possible loggerhead and leatherback mortality was quantified and mapped spatially. Loggerheads were mostly exposed to and had the highest mortality in the artisanal fisheries in the SWIO (> 1000 per annum), inshore trawling (ca. 41 per annum), shark nets (protective gill nets) (21.6 ± 6.7 per annum) and the pelagic longline fishery (5.0 ± 4.4 per annum). In contrast, leatherbacks with a pelagic lifestyle, were mostly exposed to pelagic longline fisheries (7.8 ± 7.8 per annum). A spatial analysis of fishing activities indicated that leatherback home ranges overlapped 41percent with pelagic longline fishing activity in the SA EEZ, whereas the overlap between pelagic longliners and loggerhead home ranges was 29 percent. The quantified sources of mortality provide some explanation for the trend in the loggerhead nesting population but not the trend in the leatherback nesting population. Hatchling survivorship to adulthood was estimated to determine the viability of the two nesting populations as well as to determine whether offshore mortality was responsible for the difference in recovery of the two populations. Loggerhead hatchling survivorship to adulthood was estimated at between 2 and 10 per 1000 hatchlings, the minimum requirement for an increasing population. The adopted sophisticated model shows that leatherbacks have a survival rate of 5 to 10 per 1 000 hatchlings. However, this suggests that the population is increasing, but the leatherback population is stable. Perhaps the age to maturity of SA leatherbacks is greater than 12 years, or fisheries-related mortality affects younger age classes than initially thought. It is therefore recommended that the turtle monitoring area is extended to include other potential nesting grounds. In addition, observer or monitoring programs for commercial as well as artisanal fisheries needs to be extended throughout the SWIO to quantify sea turtle mortality. Ultimately a comprehensive multi-regional approach is required for the conservation of these highly migratory species.
- Full Text:
- Date Issued: 2012
Feeding dynamics of suspension-feeders in the nearshore marine environment adjacent to two contrasting estuaries in the Eastern Cape, South Africa
- Authors: Vermeulen, Ilke
- Date: 2012
- Subjects: Suspension feeders -- South Africa -- Eastern Cape , Suspension feeders -- Food -- South Africa -- Eastern Cape , Coastal organisms -- South Africa -- Eastern Cape , Streamflow -- South Africa -- Eastern Cape , Tetraclitidae -- South Africa -- Eastern Cape , Mexilhao mussel -- South Africa -- Eastern Cape , Polychaeta -- South Africa -- Eastern Cape , Estuarine health -- South Africa -- Eastern Cape , Estuaries -- Freshwater inflow -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5916 , http://hdl.handle.net/10962/d1016242
- Description: Coastal transition zones form important interlinking regions where marine ecosystems, rivers and estuarine environments significantly influence each other. Coastal rocky shores are key habitats that sustain a variety of primary producers and invertebrates and due to the dynamic nature of coastal ecosystems, suspension-feeders on rocky shores can be influenced by an array of autochthonous and allochthonous food sources. Fatty acid and stable isotope trophic markers were employed to distinguish between regional and temporal changes in the potential food sources to rocky shore suspension-feeders in the Eastern Cape Province of South Africa. The primary aim was to assess the spatial and temporal influences of contrasting river flows on the available food sources to three indigenous coastal suspensionfeeders, namely the volcano barnacle Tetraclita serrata, brown mussel Perna perna and tubebuilding polychaete Gunnarea capensis. This was done by examining the intra- and interspecific changes in the fatty acid and stable isotope signatures of the barnacles, mussels and polychaetes in the adjacent marine environment of a freshwater-restricted (Kariega) and freshwater-dominated (Great Fish) estuary during austral summer and winter. Multivariate and Kruskal-Wallis analyses of variance on the fatty acid and isotopic signatures, respectively, identified significant regional changes in the barnacles and mussels, while only stable isotopes distinguished between the Kariega and Great Fish polychaetes (P < 0.05). In addition, significant temporal changes were observed in consumer fatty acids and isotope values in both regions (P < 0.05). Bacterial sources, detritus and phytoplankton assemblages, which are influenced by hydrology and vegetation, differed between regions and were mainly responsible for the regional and temporal separations. Principal component analyses on the consumer fatty acid signatures distinguished between animals situated upstream (i.e. north) and downstream (i.e. south) of the Kariega Estuary mouth in summer. The north/south separation was mainly due to greater contributions of diatoms to northernlocated animals and dinoflagellates and detritus to southern-located consumers. In addition, the south-flowing Agulhas Current on the eastern shores of southern Africa appeared to influence the north/south separation in the Kariega region, as water leaving the estuary was probably entrained into the south-easterly flowing currents, thereby depositing estuarinederived detritus to southern populations. In general, diatoms and detritus were essential food sources to the filter-feeders in summer, and flagellates, diatoms, zooplankton and detritus were important in winter. Coastal macroalgae was a key food source in the Kariega and Great Fish regions during both seasons. Consistently large levels of diatom markers (16:1n-7 and 20:5n-3) and dinoflagellate markers (22:6n-3) in consumer tissues in the Kariega and Great Fish regions identified that phytoplankton was their dominant food source. The barnacles, mussels and polychaetes had similar fatty acid markers and a fairly narrow δ¹³C range (-16.5 to -14.4 ‰), suggesting that they probably consumed similar food. Carbon isotope analyses, however, separated the suspension-feeders into slightly depleted (barnacles; -16.5 to -16.1 ‰), intermediate (mussels; -15.8 to -15.0 ‰) and enriched (polychaetes; -15.0 to -14.4 ‰) consumers, but did not provide conclusive evidence of their preferences for specific phytoplankton. Conversely, fatty acid analyses highlighted that barnacles and mussels had greater proportions of dinoflagellate markers (22:6n-3; 7.0-15.3 % TFA), while polychaetes had larger diatom levels (20:5n-3; 15.1-22.2 % TFA). In addition, all three species had consistently large contributions from bacterial fatty acids (15:0, i-16:0, 17:0 and i-18:0; 4.2-13.6 % TFA) in summer and winter, and large proportions of saturated fatty acids (33.3-53.1 % TFA) including those with 14 to 18 carbons, indicating that bacterial and detritus food sources played an important role in their diets. Barnacles had small levels of terrestrial markers (18:2n-6 and 18:3n-3; <2.5 % TFA) and demonstrated increased omnivorous feeding compared with the other suspension-feeders [increased levels of 20:1n-11 and 20:1n-9, higher 18:1n-9/18:1n-7 ratios at ~2.1, enriched δ¹⁵N values at ~10.6 ‰; zooplankton (potentially including microzooplankton, larvae and protists) contribution of up to 61 % of the diet]. Mussels contained significant proportions of the terrestrial markers (18:2n-6 and 18:3n-3; >2.5 % TFA) and exhibited intermediate omnivory (intermediate levels of 20:1n-11 and 20:1n-9, intermediate 18:1n-9/18:1n-7 ratios at ~1.3, less enriched δ¹⁵N values at ~7.9 ‰; zooplankton contribution of 10-15 % of the diet). The more depleted nitrogen signatures in the mussels relative to the barnacles and polychaetes possibly illustrated a stronger preference for autotrophic food. Polychaetes mainly consumed plant food sources (i.e. microalgae, macroalgae and detritus; high levels of i-18:0, 18:1n-9, 18:4n-3 and 20:5n-3) and displayed little omnivory (low levels of 20:1n-11 and 20:1n-9, low 18:1n-9/18:1n-7 ratios at ~0.4, intermediate δ¹⁵N values at ~9.1 ‰; zooplankton contribution of <10 % of the diet). The barnacles, mussels and polychaetes are all suspension-feeders, originally presumed to consume the same food sources. The variations observed among the species, therefore, may result from differences in the proportional contributions of the various food sources to their diets as well as distinctions in metabolism. The distinct changes in the fatty acid and stable isotope signatures in all three filter-feeders in the Kariega and Great Fish regions are likely influenced by the diversity in regional vegetation and hydrology in the different systems, combined with interspecific differences in resource partitioning among the species.
- Full Text:
- Date Issued: 2012
- Authors: Vermeulen, Ilke
- Date: 2012
- Subjects: Suspension feeders -- South Africa -- Eastern Cape , Suspension feeders -- Food -- South Africa -- Eastern Cape , Coastal organisms -- South Africa -- Eastern Cape , Streamflow -- South Africa -- Eastern Cape , Tetraclitidae -- South Africa -- Eastern Cape , Mexilhao mussel -- South Africa -- Eastern Cape , Polychaeta -- South Africa -- Eastern Cape , Estuarine health -- South Africa -- Eastern Cape , Estuaries -- Freshwater inflow -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5916 , http://hdl.handle.net/10962/d1016242
- Description: Coastal transition zones form important interlinking regions where marine ecosystems, rivers and estuarine environments significantly influence each other. Coastal rocky shores are key habitats that sustain a variety of primary producers and invertebrates and due to the dynamic nature of coastal ecosystems, suspension-feeders on rocky shores can be influenced by an array of autochthonous and allochthonous food sources. Fatty acid and stable isotope trophic markers were employed to distinguish between regional and temporal changes in the potential food sources to rocky shore suspension-feeders in the Eastern Cape Province of South Africa. The primary aim was to assess the spatial and temporal influences of contrasting river flows on the available food sources to three indigenous coastal suspensionfeeders, namely the volcano barnacle Tetraclita serrata, brown mussel Perna perna and tubebuilding polychaete Gunnarea capensis. This was done by examining the intra- and interspecific changes in the fatty acid and stable isotope signatures of the barnacles, mussels and polychaetes in the adjacent marine environment of a freshwater-restricted (Kariega) and freshwater-dominated (Great Fish) estuary during austral summer and winter. Multivariate and Kruskal-Wallis analyses of variance on the fatty acid and isotopic signatures, respectively, identified significant regional changes in the barnacles and mussels, while only stable isotopes distinguished between the Kariega and Great Fish polychaetes (P < 0.05). In addition, significant temporal changes were observed in consumer fatty acids and isotope values in both regions (P < 0.05). Bacterial sources, detritus and phytoplankton assemblages, which are influenced by hydrology and vegetation, differed between regions and were mainly responsible for the regional and temporal separations. Principal component analyses on the consumer fatty acid signatures distinguished between animals situated upstream (i.e. north) and downstream (i.e. south) of the Kariega Estuary mouth in summer. The north/south separation was mainly due to greater contributions of diatoms to northernlocated animals and dinoflagellates and detritus to southern-located consumers. In addition, the south-flowing Agulhas Current on the eastern shores of southern Africa appeared to influence the north/south separation in the Kariega region, as water leaving the estuary was probably entrained into the south-easterly flowing currents, thereby depositing estuarinederived detritus to southern populations. In general, diatoms and detritus were essential food sources to the filter-feeders in summer, and flagellates, diatoms, zooplankton and detritus were important in winter. Coastal macroalgae was a key food source in the Kariega and Great Fish regions during both seasons. Consistently large levels of diatom markers (16:1n-7 and 20:5n-3) and dinoflagellate markers (22:6n-3) in consumer tissues in the Kariega and Great Fish regions identified that phytoplankton was their dominant food source. The barnacles, mussels and polychaetes had similar fatty acid markers and a fairly narrow δ¹³C range (-16.5 to -14.4 ‰), suggesting that they probably consumed similar food. Carbon isotope analyses, however, separated the suspension-feeders into slightly depleted (barnacles; -16.5 to -16.1 ‰), intermediate (mussels; -15.8 to -15.0 ‰) and enriched (polychaetes; -15.0 to -14.4 ‰) consumers, but did not provide conclusive evidence of their preferences for specific phytoplankton. Conversely, fatty acid analyses highlighted that barnacles and mussels had greater proportions of dinoflagellate markers (22:6n-3; 7.0-15.3 % TFA), while polychaetes had larger diatom levels (20:5n-3; 15.1-22.2 % TFA). In addition, all three species had consistently large contributions from bacterial fatty acids (15:0, i-16:0, 17:0 and i-18:0; 4.2-13.6 % TFA) in summer and winter, and large proportions of saturated fatty acids (33.3-53.1 % TFA) including those with 14 to 18 carbons, indicating that bacterial and detritus food sources played an important role in their diets. Barnacles had small levels of terrestrial markers (18:2n-6 and 18:3n-3; <2.5 % TFA) and demonstrated increased omnivorous feeding compared with the other suspension-feeders [increased levels of 20:1n-11 and 20:1n-9, higher 18:1n-9/18:1n-7 ratios at ~2.1, enriched δ¹⁵N values at ~10.6 ‰; zooplankton (potentially including microzooplankton, larvae and protists) contribution of up to 61 % of the diet]. Mussels contained significant proportions of the terrestrial markers (18:2n-6 and 18:3n-3; >2.5 % TFA) and exhibited intermediate omnivory (intermediate levels of 20:1n-11 and 20:1n-9, intermediate 18:1n-9/18:1n-7 ratios at ~1.3, less enriched δ¹⁵N values at ~7.9 ‰; zooplankton contribution of 10-15 % of the diet). The more depleted nitrogen signatures in the mussels relative to the barnacles and polychaetes possibly illustrated a stronger preference for autotrophic food. Polychaetes mainly consumed plant food sources (i.e. microalgae, macroalgae and detritus; high levels of i-18:0, 18:1n-9, 18:4n-3 and 20:5n-3) and displayed little omnivory (low levels of 20:1n-11 and 20:1n-9, low 18:1n-9/18:1n-7 ratios at ~0.4, intermediate δ¹⁵N values at ~9.1 ‰; zooplankton contribution of <10 % of the diet). The barnacles, mussels and polychaetes are all suspension-feeders, originally presumed to consume the same food sources. The variations observed among the species, therefore, may result from differences in the proportional contributions of the various food sources to their diets as well as distinctions in metabolism. The distinct changes in the fatty acid and stable isotope signatures in all three filter-feeders in the Kariega and Great Fish regions are likely influenced by the diversity in regional vegetation and hydrology in the different systems, combined with interspecific differences in resource partitioning among the species.
- Full Text:
- Date Issued: 2012
Feeding ecology and dietary patterns of the Indo-Pacific bottlenose dolphin (Tursiops Aduncus) off KwaZulu-Natal, South Africa
- Authors: Kaiser, Sabina Marie Luise
- Date: 2012
- Subjects: Bottlenose dolphin -- Ecology -- South Africa -- KwaZulu-Natal , Bottlenose dolphin -- Feeding and feeds -- South Africa -- KwaZulu-Natal
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10710 , http://hdl.handle.net/10948/d1011509 , Bottlenose dolphin -- Ecology -- South Africa -- KwaZulu-Natal , Bottlenose dolphin -- Feeding and feeds -- South Africa -- KwaZulu-Natal
- Description: The Indo-Pacific bottlenose dolphin (Tursiops aduncus) is a common top predator along the KwaZulu-Natal coast. There have been several dietary studies done on this species, most of which have classed this predator as an opportunistic feeder preying on the most abundant prey species in that area. In KwaZulu-Natal (KZN) a dietary study was carried out in 1990, but there have been no followup studies to examine potential changes in diet. A number of bottlenose dolphins get entangled in the shark-nets set off the KZN coast each year. These dolphins provide a valuable source of information about different aspects of the ecology of this population. The aim of this study was to examine the diet of bottlenose dolphins off the KwaZulu-Natal coast with particular emphasis on temporal change in diet composition. The contents of 102 stomachs of bottlenose dolphins (58 females, 44 males) accidentally caught in the shark nets between 1985 and 2010 were analysed, and compared to historical data from dolphins caught between 1975 and 1986. A total of 94 different prey species from 37 families (32 fish families, five cephalopod families) were identified in the stomach contents. Teleosts made up the most important prey group in numbers and weight constituting 84 percent and 67 percent, respectively, while cephalopods made up 15 percent of the number and 30 percent of the weight of the prey items. Multi Dimensional Scaling (MDS) indicated that there is no resource partitioning between males and females, but significant partitioning between juveniles and adults. Six prey species contributed more than 60 percent of the weight of all prey species in the diet. These species are the most important according to their calculated index of relative importance score and are, in order of importance: Sepia sp. (cuttlefish)., Pomadasys olivaceus (piggy), Trachurus delagoa (maasbanker), Scomber japonicus (mackeral), Loligo sp. (squid). and Pagellus bellottii natalensis (pandora or red tjor-tjor). When compared to historic data, no changes in the main prey species has occurred, but the piggy has decreased in importance (1976-1986 percent IRI=45; 2000-2010 percent IRI=22), while the maasbanker has increased (1975-1986, percent RI=4; 2000-2010 percent RI=20). Stable carbon and nitrogen isotope analysis performed on tissue from the teeth of T. aduncus caught in the shark nets between 1970 and 2010 showed that there has been no significant shift in trophic level (δ15N) or in primary prey source (δ13C) over this time. The isotopic signatures of different tissues from an individual were used to create a base line and describe the differences in tissue signatures. Blubber was found to be significantly different from skin and muscle, which were found to be isotopically similar. Skin from remote biopsies can therefore probably be used in place of muscle for isotopic studies of bottlenose dolphins, while caution should be taken when using blubber. Given that T. aduncus is still listed as “data deficient” by the International Union for the Conservation of Nature (IUCN), and the potential for climate change and expanding fisheries to alter the distribution and abundance of their prey species, long term dietary studies such as this are important in order for their effective conservation and management.
- Full Text:
- Date Issued: 2012
- Authors: Kaiser, Sabina Marie Luise
- Date: 2012
- Subjects: Bottlenose dolphin -- Ecology -- South Africa -- KwaZulu-Natal , Bottlenose dolphin -- Feeding and feeds -- South Africa -- KwaZulu-Natal
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10710 , http://hdl.handle.net/10948/d1011509 , Bottlenose dolphin -- Ecology -- South Africa -- KwaZulu-Natal , Bottlenose dolphin -- Feeding and feeds -- South Africa -- KwaZulu-Natal
- Description: The Indo-Pacific bottlenose dolphin (Tursiops aduncus) is a common top predator along the KwaZulu-Natal coast. There have been several dietary studies done on this species, most of which have classed this predator as an opportunistic feeder preying on the most abundant prey species in that area. In KwaZulu-Natal (KZN) a dietary study was carried out in 1990, but there have been no followup studies to examine potential changes in diet. A number of bottlenose dolphins get entangled in the shark-nets set off the KZN coast each year. These dolphins provide a valuable source of information about different aspects of the ecology of this population. The aim of this study was to examine the diet of bottlenose dolphins off the KwaZulu-Natal coast with particular emphasis on temporal change in diet composition. The contents of 102 stomachs of bottlenose dolphins (58 females, 44 males) accidentally caught in the shark nets between 1985 and 2010 were analysed, and compared to historical data from dolphins caught between 1975 and 1986. A total of 94 different prey species from 37 families (32 fish families, five cephalopod families) were identified in the stomach contents. Teleosts made up the most important prey group in numbers and weight constituting 84 percent and 67 percent, respectively, while cephalopods made up 15 percent of the number and 30 percent of the weight of the prey items. Multi Dimensional Scaling (MDS) indicated that there is no resource partitioning between males and females, but significant partitioning between juveniles and adults. Six prey species contributed more than 60 percent of the weight of all prey species in the diet. These species are the most important according to their calculated index of relative importance score and are, in order of importance: Sepia sp. (cuttlefish)., Pomadasys olivaceus (piggy), Trachurus delagoa (maasbanker), Scomber japonicus (mackeral), Loligo sp. (squid). and Pagellus bellottii natalensis (pandora or red tjor-tjor). When compared to historic data, no changes in the main prey species has occurred, but the piggy has decreased in importance (1976-1986 percent IRI=45; 2000-2010 percent IRI=22), while the maasbanker has increased (1975-1986, percent RI=4; 2000-2010 percent RI=20). Stable carbon and nitrogen isotope analysis performed on tissue from the teeth of T. aduncus caught in the shark nets between 1970 and 2010 showed that there has been no significant shift in trophic level (δ15N) or in primary prey source (δ13C) over this time. The isotopic signatures of different tissues from an individual were used to create a base line and describe the differences in tissue signatures. Blubber was found to be significantly different from skin and muscle, which were found to be isotopically similar. Skin from remote biopsies can therefore probably be used in place of muscle for isotopic studies of bottlenose dolphins, while caution should be taken when using blubber. Given that T. aduncus is still listed as “data deficient” by the International Union for the Conservation of Nature (IUCN), and the potential for climate change and expanding fisheries to alter the distribution and abundance of their prey species, long term dietary studies such as this are important in order for their effective conservation and management.
- Full Text:
- Date Issued: 2012
Finite element analysis of the heat transfer in friction stir welding with experimental validation
- Authors: Vosloo, Natalie
- Date: 2012
- Subjects: Finite element method , Heat -- Transmission , Friction welding
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10511 , http://hdl.handle.net/10948/d1019981
- Description: Friction stir welding is a relatively new joining process. The heat transfer involved is crucial in determining the quality of the weld. Experimenrtal data, though important, does not provide enough information about the heat transfer process and experiments can be costly and time consuming. A numerical model, using the finite element method, was developed to stimulate the heat transfer in the workpiece in which the heat generation due to friction and plastic deformation was modelled as a surface heat flux boundary condition. This model was applied to Aluminium AL6082-T6 and Titanium Ti6A1-4V for different welding condiitions. Results were validated with experimental results. The model was shown to give better predictions of the maximum temperatures at locations in the workpiece than the overall temperature trend. A parametric study was also performed on the Aluminium model in order o predict temperature fields of the workpiece for welding conditions that were additional to those undertaken experimentally. It was found that rotational speed had a larger effect on the change in temperature than the feed rate. From the parametric study it was also clear that lower rotational speeds (300 to 660 rpm) had a greater effect on the change in temperature than the higher rotational speeds (840 to 1200 rpm). It was concluded that the model was well suited for the estimation of temperatures involved in the FSw of Aluminium Al6082-T6 but was not as accurate when applied to the FSW of Titanium.
- Full Text:
- Date Issued: 2012
- Authors: Vosloo, Natalie
- Date: 2012
- Subjects: Finite element method , Heat -- Transmission , Friction welding
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10511 , http://hdl.handle.net/10948/d1019981
- Description: Friction stir welding is a relatively new joining process. The heat transfer involved is crucial in determining the quality of the weld. Experimenrtal data, though important, does not provide enough information about the heat transfer process and experiments can be costly and time consuming. A numerical model, using the finite element method, was developed to stimulate the heat transfer in the workpiece in which the heat generation due to friction and plastic deformation was modelled as a surface heat flux boundary condition. This model was applied to Aluminium AL6082-T6 and Titanium Ti6A1-4V for different welding condiitions. Results were validated with experimental results. The model was shown to give better predictions of the maximum temperatures at locations in the workpiece than the overall temperature trend. A parametric study was also performed on the Aluminium model in order o predict temperature fields of the workpiece for welding conditions that were additional to those undertaken experimentally. It was found that rotational speed had a larger effect on the change in temperature than the feed rate. From the parametric study it was also clear that lower rotational speeds (300 to 660 rpm) had a greater effect on the change in temperature than the higher rotational speeds (840 to 1200 rpm). It was concluded that the model was well suited for the estimation of temperatures involved in the FSw of Aluminium Al6082-T6 but was not as accurate when applied to the FSW of Titanium.
- Full Text:
- Date Issued: 2012
Formulation and stability testing of eye drop preparations containing phenylephrine hydrochloride
- Authors: Okafor, Chinedum Oluchukwu
- Date: 2012
- Subjects: Phenylpropanolamine , Viscosity , Glycerin
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10164 , http://hdl.handle.net/10948/d1020985
- Description: Phenylephrine hydrochloride is a potent adrenergic agent and β-receptor sympathomimetic drug, used in its optically active form (Pandey et al., 2003; Pandey et al., 2006). As an α1-adrenergic receptor agonist, phenylephrine hydrochloride is used ocularly as a decongestant for uveitis and as an agent to dilate the pupil (Lang, 1995). High intraocular doses have been reported to cause tachycardia, hypertension, and headache. These side effects are caused by large amounts of the drop draining into the nasal cavity. Eye drops that contain phenylephrine hydrochloride have proven to have low intra-ocular bioavailability because of a short contact time with the eyes which reduces the amount of drug reaching the site of action. Formulations of phenylephrine hydrochloride eye drops have varying shelf-lives of approximately two to four years. The aim of this study was to formulate and manufacture an eye drop product containing phenylephrine hydrochloride. Important characteristics that were targeted were increased ocular absorption by increasing the viscosity of the product and reduced degradation of phenylephrine hydrochloride. A variety of phenylephrine hydrochloride formulations were manufactured on a laboratory scale using hydroxypropyl methylcellulose (HPMC), glycerol, and sodium carboxy methylcellulose as viscosity modifying agents (VMA). The concentration of phenylephrine hydrochloride was ten percent. Ten millimeters of each formulation was made in triplicate. The quantity in each was evaluated using a previously validated high performance (pressure) liquid chromatography method. Physicochemical properties including pH and colour were also evaluated. Stability was assessed using real time and accelerated stability conditions in accordance with the International Conference on Harmonization (ICH) guidelines. Formulations containing hydroxypropyl methylcellulose (HPMC) as the viscosity modifying agents proved to be stable under all storage conditions when compared with formulations containing other viscosity modifying agents (VMA). However, sodium citrate dihydrate; sodium metabisulphite and EDTA also stabilized the formulations to a certain extent Changes in the appearance and colour of products containing glycerol under accelerated storage conditions were observed. The sodium carboxy methylcellulose (SCMC) containing formulation was found to be physically and chemically stable in two conditions, namely 30 °C/65 percent RH and 25 °C/60 percent RH. The formulations containing hydroxypropyl methylcellulose along with an antioxidant showed to be most stable as it remained aesthetically pleasing did not change colour and did not have a reduction in phenylephrine hydrochloride concentrations. This meant that phenylephrine hydrochloride did not degrade while the viscosity modifying agents remained stable. Rheological tests showed differences in the viscosities of the formulations as glycerol had increased in viscosity over time while HMPC and SCMC displayed relative similarities. The formulations were compared to a marketed eye drop containing polyvinyl alcohol as a VMA. After rheological analysis the formulation containing HPMC displayed better viscosity than the product with polyvinyl alcohol. The preservatives in the formulations were active against the microbial organisms use to challenged them.
- Full Text:
- Date Issued: 2012
- Authors: Okafor, Chinedum Oluchukwu
- Date: 2012
- Subjects: Phenylpropanolamine , Viscosity , Glycerin
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10164 , http://hdl.handle.net/10948/d1020985
- Description: Phenylephrine hydrochloride is a potent adrenergic agent and β-receptor sympathomimetic drug, used in its optically active form (Pandey et al., 2003; Pandey et al., 2006). As an α1-adrenergic receptor agonist, phenylephrine hydrochloride is used ocularly as a decongestant for uveitis and as an agent to dilate the pupil (Lang, 1995). High intraocular doses have been reported to cause tachycardia, hypertension, and headache. These side effects are caused by large amounts of the drop draining into the nasal cavity. Eye drops that contain phenylephrine hydrochloride have proven to have low intra-ocular bioavailability because of a short contact time with the eyes which reduces the amount of drug reaching the site of action. Formulations of phenylephrine hydrochloride eye drops have varying shelf-lives of approximately two to four years. The aim of this study was to formulate and manufacture an eye drop product containing phenylephrine hydrochloride. Important characteristics that were targeted were increased ocular absorption by increasing the viscosity of the product and reduced degradation of phenylephrine hydrochloride. A variety of phenylephrine hydrochloride formulations were manufactured on a laboratory scale using hydroxypropyl methylcellulose (HPMC), glycerol, and sodium carboxy methylcellulose as viscosity modifying agents (VMA). The concentration of phenylephrine hydrochloride was ten percent. Ten millimeters of each formulation was made in triplicate. The quantity in each was evaluated using a previously validated high performance (pressure) liquid chromatography method. Physicochemical properties including pH and colour were also evaluated. Stability was assessed using real time and accelerated stability conditions in accordance with the International Conference on Harmonization (ICH) guidelines. Formulations containing hydroxypropyl methylcellulose (HPMC) as the viscosity modifying agents proved to be stable under all storage conditions when compared with formulations containing other viscosity modifying agents (VMA). However, sodium citrate dihydrate; sodium metabisulphite and EDTA also stabilized the formulations to a certain extent Changes in the appearance and colour of products containing glycerol under accelerated storage conditions were observed. The sodium carboxy methylcellulose (SCMC) containing formulation was found to be physically and chemically stable in two conditions, namely 30 °C/65 percent RH and 25 °C/60 percent RH. The formulations containing hydroxypropyl methylcellulose along with an antioxidant showed to be most stable as it remained aesthetically pleasing did not change colour and did not have a reduction in phenylephrine hydrochloride concentrations. This meant that phenylephrine hydrochloride did not degrade while the viscosity modifying agents remained stable. Rheological tests showed differences in the viscosities of the formulations as glycerol had increased in viscosity over time while HMPC and SCMC displayed relative similarities. The formulations were compared to a marketed eye drop containing polyvinyl alcohol as a VMA. After rheological analysis the formulation containing HPMC displayed better viscosity than the product with polyvinyl alcohol. The preservatives in the formulations were active against the microbial organisms use to challenged them.
- Full Text:
- Date Issued: 2012
Genetic algorithm for Artificial Neural Network training for the purpose of Automated Part Recognition
- Authors: Buys, Stefan
- Date: 2012
- Subjects: Genetic algorithms , Software architecture
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:9648 , http://hdl.handle.net/10948/d1008356 , Genetic algorithms , Software architecture
- Description: Object or part recognition is of major interest in industrial environments. Current methods implement expensive camera based solutions. There is a need for a cost effective alternative to be developed. One of the proposed methods is to overcome the hardware, camera, problem by implementing a software solution. Artificial Neural Networks (ANN) are to be used as the underlying intelligent software as they have high tolerance for noise and have the ability to generalize. A colleague has implemented a basic ANN based system comprising of an ANN and three cost effective laser distance sensors. However, the system is only able to identify 3 different parts and needed hard coding changes made by trial and error. This is not practical for industrial use in a production environment where there are a large quantity of different parts to be identified that change relatively regularly. The ability to easily train more parts is required. Difficulties associated with traditional mathematically guided training methods are discussed, which leads to the development of a Genetic Algorithm (GA) based evolutionary training method that overcomes these difficulties and makes accurate part recognition possible. An ANN hybridised with GA training is introduced and a general solution encoding scheme which is used to encode the required ANN connection weights. Experimental tests were performed in order to determine the ideal GA performance and control parameters as studies have indicated that different GA control parameters can lead to large differences in training accuracy. After performing these tests, the training accuracy was analyzed by investigation into GA performance as well as hardware based part recognition performance. This analysis identified the ideal GA control parameters when training an ANN for the purpose of part recognition and showed that the ANN generally trained well and could generalize well on data not presented to it during training.
- Full Text:
- Date Issued: 2012
- Authors: Buys, Stefan
- Date: 2012
- Subjects: Genetic algorithms , Software architecture
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:9648 , http://hdl.handle.net/10948/d1008356 , Genetic algorithms , Software architecture
- Description: Object or part recognition is of major interest in industrial environments. Current methods implement expensive camera based solutions. There is a need for a cost effective alternative to be developed. One of the proposed methods is to overcome the hardware, camera, problem by implementing a software solution. Artificial Neural Networks (ANN) are to be used as the underlying intelligent software as they have high tolerance for noise and have the ability to generalize. A colleague has implemented a basic ANN based system comprising of an ANN and three cost effective laser distance sensors. However, the system is only able to identify 3 different parts and needed hard coding changes made by trial and error. This is not practical for industrial use in a production environment where there are a large quantity of different parts to be identified that change relatively regularly. The ability to easily train more parts is required. Difficulties associated with traditional mathematically guided training methods are discussed, which leads to the development of a Genetic Algorithm (GA) based evolutionary training method that overcomes these difficulties and makes accurate part recognition possible. An ANN hybridised with GA training is introduced and a general solution encoding scheme which is used to encode the required ANN connection weights. Experimental tests were performed in order to determine the ideal GA performance and control parameters as studies have indicated that different GA control parameters can lead to large differences in training accuracy. After performing these tests, the training accuracy was analyzed by investigation into GA performance as well as hardware based part recognition performance. This analysis identified the ideal GA control parameters when training an ANN for the purpose of part recognition and showed that the ANN generally trained well and could generalize well on data not presented to it during training.
- Full Text:
- Date Issued: 2012
GPF : a framework for general packet classification on GPU co-processors
- Authors: Nottingham, Alastair
- Date: 2012
- Subjects: Graphics processing units , Coprocessors , Computer network protocols , Computer networks -- Security measures , NVIDIA Corporation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4661 , http://hdl.handle.net/10962/d1006662 , Graphics processing units , Coprocessors , Computer network protocols , Computer networks -- Security measures , NVIDIA Corporation
- Description: This thesis explores the design and experimental implementation of GPF, a novel protocol-independent, multi-match packet classification framework. This framework is targeted and optimised for flexible, efficient execution on NVIDIA GPU platforms through the CUDA API, but should not be difficult to port to other platforms, such as OpenCL, in the future. GPF was conceived and developed in order to accelerate classification of large packet capture files, such as those collected by Network Telescopes. It uses a multiphase SIMD classification process which exploits both the parallelism of packet sets and the redundancy in filter programs, in order to classify packet captures against multiple filters at extremely high rates. The resultant framework - comprised of classification, compilation and buffering components - efficiently leverages GPU resources to classify arbitrary protocols, and return multiple filter results for each packet. The classification functions described were verified and evaluated by testing an experimental prototype implementation against several filter programs, of varying complexity, on devices from three GPU platform generations. In addition to the significant speedup achieved in processing results, analysis indicates that the prototype classification functions perform predictably, and scale linearly with respect to both packet count and filter complexity. Furthermore, classification throughput (packets/s) remained essentially constant regardless of the underlying packet data, and thus the effective data rate when classifying a particular filter was heavily influenced by the average size of packets in the processed capture. For example: in the trivial case of classifying all IPv4 packets ranging in size from 70 bytes to 1KB, the observed data rate achieved by the GPU classification kernels ranged from 60Gbps to 900Gbps on a GTX 275, and from 220Gbps to 3.3Tbps on a GTX 480. In the less trivial case of identifying all ARP, TCP, UDP and ICMP packets for both IPv4 and IPv6 protocols, the effective data rates ranged from 15Gbps to 220Gbps (GTX 275), and from 50Gbps to 740Gbps (GTX 480), for 70B and 1KB packets respectively. , LaTeX with hyperref package
- Full Text:
- Date Issued: 2012
- Authors: Nottingham, Alastair
- Date: 2012
- Subjects: Graphics processing units , Coprocessors , Computer network protocols , Computer networks -- Security measures , NVIDIA Corporation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4661 , http://hdl.handle.net/10962/d1006662 , Graphics processing units , Coprocessors , Computer network protocols , Computer networks -- Security measures , NVIDIA Corporation
- Description: This thesis explores the design and experimental implementation of GPF, a novel protocol-independent, multi-match packet classification framework. This framework is targeted and optimised for flexible, efficient execution on NVIDIA GPU platforms through the CUDA API, but should not be difficult to port to other platforms, such as OpenCL, in the future. GPF was conceived and developed in order to accelerate classification of large packet capture files, such as those collected by Network Telescopes. It uses a multiphase SIMD classification process which exploits both the parallelism of packet sets and the redundancy in filter programs, in order to classify packet captures against multiple filters at extremely high rates. The resultant framework - comprised of classification, compilation and buffering components - efficiently leverages GPU resources to classify arbitrary protocols, and return multiple filter results for each packet. The classification functions described were verified and evaluated by testing an experimental prototype implementation against several filter programs, of varying complexity, on devices from three GPU platform generations. In addition to the significant speedup achieved in processing results, analysis indicates that the prototype classification functions perform predictably, and scale linearly with respect to both packet count and filter complexity. Furthermore, classification throughput (packets/s) remained essentially constant regardless of the underlying packet data, and thus the effective data rate when classifying a particular filter was heavily influenced by the average size of packets in the processed capture. For example: in the trivial case of classifying all IPv4 packets ranging in size from 70 bytes to 1KB, the observed data rate achieved by the GPU classification kernels ranged from 60Gbps to 900Gbps on a GTX 275, and from 220Gbps to 3.3Tbps on a GTX 480. In the less trivial case of identifying all ARP, TCP, UDP and ICMP packets for both IPv4 and IPv6 protocols, the effective data rates ranged from 15Gbps to 220Gbps (GTX 275), and from 50Gbps to 740Gbps (GTX 480), for 70B and 1KB packets respectively. , LaTeX with hyperref package
- Full Text:
- Date Issued: 2012
High pressure liquid chromatographic quantification of nitrile biocatalysis
- Authors: Mathiba, Kgama
- Date: 2012
- Subjects: High performance liquid chromatography , Rhodococcus , Biocatalysis , Organic compounds -- Industrial applications
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4129 , http://hdl.handle.net/10962/d1015710
- Description: Nitrile biocatalysts are of use in the chemical and pharmaceutical industries for the synthesis of carboxyamides and carboxylic acids. In particular, the application of biocatalysts in the synthesis of single enantiomer compounds is of increasing interest, but requires novel substrate specific highly stereoselective biocatalysts. Addition to the limited toolbox of known nitrile biocatalysts requires definitive characterisation of the biocatalysts through accurate determination of the substrate profiles and quantification of activity. The accurate quantification of stereoisomers chiral mixtures to determine biocatalyst stereoselectivity remains a significant challenge due to the difficulty in separating stereoisomers by physical methods. The known nitrile metabolising organism, Rhodococcus rhodochrous ATCC BAA-870, was grown in a defined medium and harvested, providing whole cell biocatalyst. Additional biomass was disrupted to provide a cell free enzyme extract, which was put through an enzyme purification protocol to provide a solution with specific activity of 351 U.mg⁻¹. A portion of the enzyme was self immobilised using the SphereZyme™ technique. The nitrile hydratase SphereZymes™ (1.2 U.mg⁻¹ initial activity) that were prepared had pH and temperature optima of 6 and 30°C respectively, and could be recovered by repeated washing. The particles retained activity in the presence of the organic solvents isooctane and n-hexadecane saturated with 50 mM phosphate buffer (pH 7.5). An initial analytical system was devised for quantification of the nitrile hydratase activity using the non-chiral substrate benzonitrile. An improved reversed phase high performance liquid chromatography method was developed to separate and quantify benzamide, benzoic acid and benzonitrile. The mobile phase consisting of 0.1% trifluoroacetic acid in H₂O and acetonitrile (70:30, %v/v), at a flow rate of 0.5 ml.ml⁻¹, 25°C, resolved all three analytes in 3.5 minutes on a Waters X-Terra MS C18 3.5μm column. UV detection was carried out at 210 nm. Analytical methods to determine activity and enantioselectivity of the whole cell biocatalyst were subsequently developed for both β-amino nitriles and β-hydroxy nitrile substrates and hydrolysis products.
- Full Text:
- Date Issued: 2012
- Authors: Mathiba, Kgama
- Date: 2012
- Subjects: High performance liquid chromatography , Rhodococcus , Biocatalysis , Organic compounds -- Industrial applications
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4129 , http://hdl.handle.net/10962/d1015710
- Description: Nitrile biocatalysts are of use in the chemical and pharmaceutical industries for the synthesis of carboxyamides and carboxylic acids. In particular, the application of biocatalysts in the synthesis of single enantiomer compounds is of increasing interest, but requires novel substrate specific highly stereoselective biocatalysts. Addition to the limited toolbox of known nitrile biocatalysts requires definitive characterisation of the biocatalysts through accurate determination of the substrate profiles and quantification of activity. The accurate quantification of stereoisomers chiral mixtures to determine biocatalyst stereoselectivity remains a significant challenge due to the difficulty in separating stereoisomers by physical methods. The known nitrile metabolising organism, Rhodococcus rhodochrous ATCC BAA-870, was grown in a defined medium and harvested, providing whole cell biocatalyst. Additional biomass was disrupted to provide a cell free enzyme extract, which was put through an enzyme purification protocol to provide a solution with specific activity of 351 U.mg⁻¹. A portion of the enzyme was self immobilised using the SphereZyme™ technique. The nitrile hydratase SphereZymes™ (1.2 U.mg⁻¹ initial activity) that were prepared had pH and temperature optima of 6 and 30°C respectively, and could be recovered by repeated washing. The particles retained activity in the presence of the organic solvents isooctane and n-hexadecane saturated with 50 mM phosphate buffer (pH 7.5). An initial analytical system was devised for quantification of the nitrile hydratase activity using the non-chiral substrate benzonitrile. An improved reversed phase high performance liquid chromatography method was developed to separate and quantify benzamide, benzoic acid and benzonitrile. The mobile phase consisting of 0.1% trifluoroacetic acid in H₂O and acetonitrile (70:30, %v/v), at a flow rate of 0.5 ml.ml⁻¹, 25°C, resolved all three analytes in 3.5 minutes on a Waters X-Terra MS C18 3.5μm column. UV detection was carried out at 210 nm. Analytical methods to determine activity and enantioselectivity of the whole cell biocatalyst were subsequently developed for both β-amino nitriles and β-hydroxy nitrile substrates and hydrolysis products.
- Full Text:
- Date Issued: 2012
How does the ungulate community respond to predation risk from cheetah (Acinonyx jubatus) in Samara Private Game Reserve?
- Authors: Makin, Douglas Ferguson
- Date: 2012
- Subjects: Ungulates -- South Africa -- Eastern Cape , Predatory animals -- Ecology -- South Africa -- Eastern Cape , Predation (Biology) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10722 , http://hdl.handle.net/10948/d1018461
- Description: Predator reintroductions are becoming increasingly more common for multiple reasons, including assisting with the conservation of a predator species, the restoration of ecosystem functions and the economic benefits of their reintroduction for ecotourism ventures. There remains however, little knowledge on prey species responses to these predator reintroductions. As such, the reintroduction of cheetah (Acinonyx jubatus) into Samara Private Game Reserve provided an opportunity to investigate prey responses to predator reintroduction across a range of spatial and temporal scales. More specifically, the aim of this study was to investigate the effect of cheetah predation risk on habitat use and behavioural responses of the resident ungulate community. Samara is divided into “predator present” and “predator absent” sections, providing the opportunity to conduct a comparative study investigating the effect of cheetah on prey responses. It was hypothesized that different ungulate species would respond differently to the presence of cheetah, depending on differences in perceived vulnerability to cheetah predation. To address this, shifts in habitat use, and behavioural responses of the ungulate community reflected at landscape and patch scale were investigated. Overall, ungulate species have not shifted habitat use since cheetah reintroduction, this was possibly related to life history strategy constraints and the need for individuals to obtain suitable forage and therefore remain in specific habitats. While no shift in habitat use was observed for the majority of ungulate species, a shift in behaviour was observed for kudu (Tragelaphus strepsiceros) at a landscape scale, where kudu dedicated more time to vigilance and less time to foraging within the predator section. In addition, individuals within smaller kudu groups were observed to be more vigilant than individuals within larger groups of kudu within the predator section. Although the other three ungulate species monitored did not increase time spent vigilant within the predator section, they still maintained relatively high levels of vigilance, potentially as a means of social monitoring. At a patch level, ungulate species responded strongly to a predator cue as a proxy for cheetah proximity, and increased time spent vigilant with a trade-off of lower foraging effort. This vigilant response was strongest for kudu. Differences in perceived predation risk were reflected within eland (Tragelaphus oryx) and kudu species demographic classes, with juvenile eland and kudu, adult female kudu and subadult female kudu spending more time vigilant within manipulated patches than respective males of each species. Kudu were also observed adopting fine-scale behavioural responses to minimize predation risk within patches. The asymmetrical prey species response to perceived predation risk from cheetah supports the hypothesis that different species respond differently to the presence of a predator. Furthermore, this study illustrated the importance of measuring prey responses to predation risk across multiple scales and highlighted the need to replicate this study for a number of different sites where predators have been reintroduced, to better understand the range of factors influencing these predator-prey interactions.
- Full Text:
- Date Issued: 2012
- Authors: Makin, Douglas Ferguson
- Date: 2012
- Subjects: Ungulates -- South Africa -- Eastern Cape , Predatory animals -- Ecology -- South Africa -- Eastern Cape , Predation (Biology) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10722 , http://hdl.handle.net/10948/d1018461
- Description: Predator reintroductions are becoming increasingly more common for multiple reasons, including assisting with the conservation of a predator species, the restoration of ecosystem functions and the economic benefits of their reintroduction for ecotourism ventures. There remains however, little knowledge on prey species responses to these predator reintroductions. As such, the reintroduction of cheetah (Acinonyx jubatus) into Samara Private Game Reserve provided an opportunity to investigate prey responses to predator reintroduction across a range of spatial and temporal scales. More specifically, the aim of this study was to investigate the effect of cheetah predation risk on habitat use and behavioural responses of the resident ungulate community. Samara is divided into “predator present” and “predator absent” sections, providing the opportunity to conduct a comparative study investigating the effect of cheetah on prey responses. It was hypothesized that different ungulate species would respond differently to the presence of cheetah, depending on differences in perceived vulnerability to cheetah predation. To address this, shifts in habitat use, and behavioural responses of the ungulate community reflected at landscape and patch scale were investigated. Overall, ungulate species have not shifted habitat use since cheetah reintroduction, this was possibly related to life history strategy constraints and the need for individuals to obtain suitable forage and therefore remain in specific habitats. While no shift in habitat use was observed for the majority of ungulate species, a shift in behaviour was observed for kudu (Tragelaphus strepsiceros) at a landscape scale, where kudu dedicated more time to vigilance and less time to foraging within the predator section. In addition, individuals within smaller kudu groups were observed to be more vigilant than individuals within larger groups of kudu within the predator section. Although the other three ungulate species monitored did not increase time spent vigilant within the predator section, they still maintained relatively high levels of vigilance, potentially as a means of social monitoring. At a patch level, ungulate species responded strongly to a predator cue as a proxy for cheetah proximity, and increased time spent vigilant with a trade-off of lower foraging effort. This vigilant response was strongest for kudu. Differences in perceived predation risk were reflected within eland (Tragelaphus oryx) and kudu species demographic classes, with juvenile eland and kudu, adult female kudu and subadult female kudu spending more time vigilant within manipulated patches than respective males of each species. Kudu were also observed adopting fine-scale behavioural responses to minimize predation risk within patches. The asymmetrical prey species response to perceived predation risk from cheetah supports the hypothesis that different species respond differently to the presence of a predator. Furthermore, this study illustrated the importance of measuring prey responses to predation risk across multiple scales and highlighted the need to replicate this study for a number of different sites where predators have been reintroduced, to better understand the range of factors influencing these predator-prey interactions.
- Full Text:
- Date Issued: 2012
Identifying volatile emissions associated with False Codling Moth infested citrus fruit
- Authors: Van der Walt, Rachel
- Date: 2012
- Subjects: Citrus -- Diseases and pests , Insect pests , Cryptophlebia leucotreta
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10343 , http://hdl.handle.net/10948/d1020056
- Description: False codling moth is a known pest of economic importance to many cultivated crops in South Africa and Africa south of the Sahara, and is particularly severe on citrus. If the fruit is infested just before harvest the chances of detecting signs of infestation are very low. As a result, the risk of packaging infested fruit and exporting them as healthy fruit is high. It is therefore a priority to develop a post-harvest technique for detection of False codling moth in citrus fruit at different levels of infestation in order to reduce phytosanitary risk. Compounds released and detected were indicative of infestation and were not insect produced but naturally produced fruit volatiles emitted at higher levels as a result of the insect within the fruit. Five major volatile compounds of interest were released by the infested oranges. These major volatile compounds include D-limonene, 3,7-dimethyl-1,3,6-octatriene, (E)-4,8-dimethyl-1,3,7-nonatriene, caryophyllene and naphthalene. Limonene was one of the most abundant volatile compounds released by the infested citrus fruit. Naphthalene, which is possibly produced due to larval feeding and development within the fruit maintained higher concentrations than controls throughout the infestation within the fruit. Naphthalene would be a good indicator of False codling moth infestation, however, not primarily for early infestation detection. A significantly higher concentration of D-limonene, 3,7-dimethyl-1,3,6-octatriene, (E)-4,8-dimethyl-1,3,7-nonatriene and naphthalene was detected using the SEP over the SPME technique. The application of an SPME procedure and the utilization of this method for detection of volatiles present in the headspace of intact infested fruit are evaluated and the possible volatile compounds diagnostic of Thaumatotibia leucotreta infestation of orange fruit and differences in volatile compound response in different orange varieties is discussed.
- Full Text:
- Date Issued: 2012
- Authors: Van der Walt, Rachel
- Date: 2012
- Subjects: Citrus -- Diseases and pests , Insect pests , Cryptophlebia leucotreta
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10343 , http://hdl.handle.net/10948/d1020056
- Description: False codling moth is a known pest of economic importance to many cultivated crops in South Africa and Africa south of the Sahara, and is particularly severe on citrus. If the fruit is infested just before harvest the chances of detecting signs of infestation are very low. As a result, the risk of packaging infested fruit and exporting them as healthy fruit is high. It is therefore a priority to develop a post-harvest technique for detection of False codling moth in citrus fruit at different levels of infestation in order to reduce phytosanitary risk. Compounds released and detected were indicative of infestation and were not insect produced but naturally produced fruit volatiles emitted at higher levels as a result of the insect within the fruit. Five major volatile compounds of interest were released by the infested oranges. These major volatile compounds include D-limonene, 3,7-dimethyl-1,3,6-octatriene, (E)-4,8-dimethyl-1,3,7-nonatriene, caryophyllene and naphthalene. Limonene was one of the most abundant volatile compounds released by the infested citrus fruit. Naphthalene, which is possibly produced due to larval feeding and development within the fruit maintained higher concentrations than controls throughout the infestation within the fruit. Naphthalene would be a good indicator of False codling moth infestation, however, not primarily for early infestation detection. A significantly higher concentration of D-limonene, 3,7-dimethyl-1,3,6-octatriene, (E)-4,8-dimethyl-1,3,7-nonatriene and naphthalene was detected using the SEP over the SPME technique. The application of an SPME procedure and the utilization of this method for detection of volatiles present in the headspace of intact infested fruit are evaluated and the possible volatile compounds diagnostic of Thaumatotibia leucotreta infestation of orange fruit and differences in volatile compound response in different orange varieties is discussed.
- Full Text:
- Date Issued: 2012
In silico characterisation of the four canonical plasmodium falciparum 70 kDa heat shock proteins
- Authors: Hatherley, Rowan
- Date: 2012
- Subjects: Heat shock proteins -- Research , Plasmodium falciparum -- Research , Plasmodium -- Research , Endoplasmic reticulum , Cytosol , Mitochondria -- Formation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4026 , http://hdl.handle.net/10962/d1004086 , Heat shock proteins -- Research , Plasmodium falciparum -- Research , Plasmodium -- Research , Endoplasmic reticulum , Cytosol , Mitochondria -- Formation
- Description: The 70 kDa heat shock proteins expressed by Plasmodium falciparum (PfHsp70s) are believed to be essential to both the survival and virulence of the malaria parasite. A total of six Hsp70 genes have been identified in the genome of P. falciparum. However, only four of these encode canonical Hsp70s, which are believed to localise predominantly in the cytosol (PfHsp70-1 and PfHsp70-x), the endoplasmic reticulum (PfHsp70-2) and mitochondria (PfHsp70-3) of the parasite. These proteins bind and release peptide substrates in an ATP-dependent manner, with the aid of a J-domain protein cochaperone and a nucleotide exchange factor (NEF). The aim of this study was to identify the residues involved in the interaction of these PfHsp70s with their peptide substrates, their J-domain cochaperones and potential NEFs. These residues were then mapped to three-dimensional (3D) structures of the proteins, modelled in three different conformations; each representing a different stage in the ATPase cycle. Additionally, these proteins were compared to different types of Hsp70s from a variety of different organisms and sequence features found to be specific to each PfHsp70 were mapped to their 3D structures. Finally, a novel modelling method was suggested, in which the structures of templates were remodelled to improve their quality before they were used in the homology modelling process. Based on the analysis of residues involved in interactions with other proteins, it was revealed that each PfHsp70 displayed features that were specific to its cellular localisation and each type of Hsp70 was predicted to interact with a different set of NEFs. The study of conserved features in each PfHsp70 revealed that PfHsp70-x displayed various sequence features atypical of both Plasmodium cytosolic Hsp70s and cytosolic Hsp70s in general. Additionally, residues conserved specifically in Hsp70s of Apicomplexa, Plasmodium and P. falciparum were identified and mapped to the each PfHsp70 model. Although these residues were too numerous to reveal any information of specific value, these models may be useful for the purposes of aiding the design of drug compounds against each PfHsp70. Finally, the novel modelling approach did show some promise. Half of the models produced using the modified templates were of a higher quality than their counterparts modelled using the original templates. This approach does still require a lot of validation work and statistical evaluation. It is hoped that it could prove to be a useful approach to homology modelling when the only templates available are poor quality structures.
- Full Text:
- Date Issued: 2012
- Authors: Hatherley, Rowan
- Date: 2012
- Subjects: Heat shock proteins -- Research , Plasmodium falciparum -- Research , Plasmodium -- Research , Endoplasmic reticulum , Cytosol , Mitochondria -- Formation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4026 , http://hdl.handle.net/10962/d1004086 , Heat shock proteins -- Research , Plasmodium falciparum -- Research , Plasmodium -- Research , Endoplasmic reticulum , Cytosol , Mitochondria -- Formation
- Description: The 70 kDa heat shock proteins expressed by Plasmodium falciparum (PfHsp70s) are believed to be essential to both the survival and virulence of the malaria parasite. A total of six Hsp70 genes have been identified in the genome of P. falciparum. However, only four of these encode canonical Hsp70s, which are believed to localise predominantly in the cytosol (PfHsp70-1 and PfHsp70-x), the endoplasmic reticulum (PfHsp70-2) and mitochondria (PfHsp70-3) of the parasite. These proteins bind and release peptide substrates in an ATP-dependent manner, with the aid of a J-domain protein cochaperone and a nucleotide exchange factor (NEF). The aim of this study was to identify the residues involved in the interaction of these PfHsp70s with their peptide substrates, their J-domain cochaperones and potential NEFs. These residues were then mapped to three-dimensional (3D) structures of the proteins, modelled in three different conformations; each representing a different stage in the ATPase cycle. Additionally, these proteins were compared to different types of Hsp70s from a variety of different organisms and sequence features found to be specific to each PfHsp70 were mapped to their 3D structures. Finally, a novel modelling method was suggested, in which the structures of templates were remodelled to improve their quality before they were used in the homology modelling process. Based on the analysis of residues involved in interactions with other proteins, it was revealed that each PfHsp70 displayed features that were specific to its cellular localisation and each type of Hsp70 was predicted to interact with a different set of NEFs. The study of conserved features in each PfHsp70 revealed that PfHsp70-x displayed various sequence features atypical of both Plasmodium cytosolic Hsp70s and cytosolic Hsp70s in general. Additionally, residues conserved specifically in Hsp70s of Apicomplexa, Plasmodium and P. falciparum were identified and mapped to the each PfHsp70 model. Although these residues were too numerous to reveal any information of specific value, these models may be useful for the purposes of aiding the design of drug compounds against each PfHsp70. Finally, the novel modelling approach did show some promise. Half of the models produced using the modified templates were of a higher quality than their counterparts modelled using the original templates. This approach does still require a lot of validation work and statistical evaluation. It is hoped that it could prove to be a useful approach to homology modelling when the only templates available are poor quality structures.
- Full Text:
- Date Issued: 2012
In vitro cytotoxic effects of selected Nigerian medicinal plant extracts on cancer cell lines
- Authors: Baatjies, Lucinda
- Date: 2012
- Subjects: Cancer -- Treatment , Cancer cells , Medicinal plants , Plant extracts , Traditional medicine , Public health
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10316 , http://hdl.handle.net/10948/d1008191 , Cancer -- Treatment , Cancer cells , Medicinal plants , Plant extracts , Traditional medicine , Public health
- Description: Cancer is a disease that imposes a heavy burden on public health and poses a challenge to science. The World Health Organization estimates that 80 percent of people in developing countries of the world rely on traditional medicine for their primary health needs, and about 85 percent of traditional medicine involves the use of plant extracts. This is particularly true in Africa where a large percentage of the population depends upon medicinal plants for health care. Therefore, detailed screening and evaluation of bioactive substances for chemotherapeutic purposes of African plants are urgently warranted. Furthermore, this will serve to validate the efficacy and safety of African traditional medicine. The current study investigated the in vitro cytotoxic effects of 17 ethanolic extracts of the following 16 plants used in traditional anticancer medicine in Nigeria: Sapium ellipticum leaves, Sapium ellipticum stembark, Combretum paniculatum, Celosia trigyna, Pupalia lappacea, Justica extensa, Hedranthera barteri leaves, Alternanthera sessilis, Ethulia conyzoides leaves, Lannea nigritana stembark, Combretum zenkeri root, Combretum molle leaves, Adenanthera parvoniana, Lannea acida, Cyathula achyranthoides, Drymaria cordata, Cyathula prostrata, against HeLa cancer cells. Five of the most promising extracts (Sapium ellipticum leaves, Combretum paniculatum, Celosia trigyna, Drymaria cordata, Cyathula prostrata) were selected for further screening against HT29 and MCF-7 cancer cells. Of the five, the first two were investigated further based on their activities in the screening phase. The S. ellipticum leaf extract yielded IC50 values of 88.60 ± 0.03 and 93.03 ± 0.03 μg/ml against HeLa and MCF-7, respectively. The toxicity was also evaluated on normal cells and an IC50 of 77.66 μg/ml was obtained for peripheral blood mononuclear cells (PBMCs). The IC50 values for proliferating and confluent Chang liver cells were both >125 μg/ml. These results suggest that the extract may be selective for specific cell types. Bio-assay guided fractionation of the S. ellipticum ethanolic extract yielded two active fractions; chloroform and ethyl acetate. Two compounds isolated from the chloroform extract were screened against the three cancer cell lines and found to be inactive. Three compounds were isolated from the ethyl acetate fraction and revealed IC50 values < 62.5 and < 31 μg/ml against MCF-7. Unfortunately these two compounds soon lost activity before any further work could be done on them and work was continued with the crude extract.
- Full Text:
- Date Issued: 2012
- Authors: Baatjies, Lucinda
- Date: 2012
- Subjects: Cancer -- Treatment , Cancer cells , Medicinal plants , Plant extracts , Traditional medicine , Public health
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10316 , http://hdl.handle.net/10948/d1008191 , Cancer -- Treatment , Cancer cells , Medicinal plants , Plant extracts , Traditional medicine , Public health
- Description: Cancer is a disease that imposes a heavy burden on public health and poses a challenge to science. The World Health Organization estimates that 80 percent of people in developing countries of the world rely on traditional medicine for their primary health needs, and about 85 percent of traditional medicine involves the use of plant extracts. This is particularly true in Africa where a large percentage of the population depends upon medicinal plants for health care. Therefore, detailed screening and evaluation of bioactive substances for chemotherapeutic purposes of African plants are urgently warranted. Furthermore, this will serve to validate the efficacy and safety of African traditional medicine. The current study investigated the in vitro cytotoxic effects of 17 ethanolic extracts of the following 16 plants used in traditional anticancer medicine in Nigeria: Sapium ellipticum leaves, Sapium ellipticum stembark, Combretum paniculatum, Celosia trigyna, Pupalia lappacea, Justica extensa, Hedranthera barteri leaves, Alternanthera sessilis, Ethulia conyzoides leaves, Lannea nigritana stembark, Combretum zenkeri root, Combretum molle leaves, Adenanthera parvoniana, Lannea acida, Cyathula achyranthoides, Drymaria cordata, Cyathula prostrata, against HeLa cancer cells. Five of the most promising extracts (Sapium ellipticum leaves, Combretum paniculatum, Celosia trigyna, Drymaria cordata, Cyathula prostrata) were selected for further screening against HT29 and MCF-7 cancer cells. Of the five, the first two were investigated further based on their activities in the screening phase. The S. ellipticum leaf extract yielded IC50 values of 88.60 ± 0.03 and 93.03 ± 0.03 μg/ml against HeLa and MCF-7, respectively. The toxicity was also evaluated on normal cells and an IC50 of 77.66 μg/ml was obtained for peripheral blood mononuclear cells (PBMCs). The IC50 values for proliferating and confluent Chang liver cells were both >125 μg/ml. These results suggest that the extract may be selective for specific cell types. Bio-assay guided fractionation of the S. ellipticum ethanolic extract yielded two active fractions; chloroform and ethyl acetate. Two compounds isolated from the chloroform extract were screened against the three cancer cell lines and found to be inactive. Three compounds were isolated from the ethyl acetate fraction and revealed IC50 values < 62.5 and < 31 μg/ml against MCF-7. Unfortunately these two compounds soon lost activity before any further work could be done on them and work was continued with the crude extract.
- Full Text:
- Date Issued: 2012
In-beam spectroscopy of ⁷²Ge
- Authors: Henninger, Katharine Rose
- Date: 2012
- Subjects: Nuclear shapes , Hyperfine structure
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5559 , http://hdl.handle.net/10962/d1018233
- Description: The high-spin states of the nucleus ⁷²Ge were populated via the ⁷°Zn(a,2n)⁷²Ge fusion-evaporation reaction at a beam-energy of 30 MeV. The ')'-decays were observed with the AFRODITE spectrometer array at iThemba LABS (Faure, South Africa). Twenty-seven new transitions, 18 new levels and 2 new bands were added to the ⁷²Ge level scheme, and several corrections were made to t he previously-known level scheme. Transitions were characterised using Directional Correlation from Oriented states (DCO) and polarisation measurements, and spin-parity assignments were made for 7 new levels. Spin and parity were also reassigned to a previously-known structure. One of the new bands was ident ified as part of an octupole-vibrational band. No tetrahedral states were observed in the scheme despite N = 40 and Z = 32 having been identified as tetrahedral magic numbers. The meaning of the new 14⁺ yrast level for the underlying structure of the ground state band is discussed
- Full Text:
- Date Issued: 2012
- Authors: Henninger, Katharine Rose
- Date: 2012
- Subjects: Nuclear shapes , Hyperfine structure
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5559 , http://hdl.handle.net/10962/d1018233
- Description: The high-spin states of the nucleus ⁷²Ge were populated via the ⁷°Zn(a,2n)⁷²Ge fusion-evaporation reaction at a beam-energy of 30 MeV. The ')'-decays were observed with the AFRODITE spectrometer array at iThemba LABS (Faure, South Africa). Twenty-seven new transitions, 18 new levels and 2 new bands were added to the ⁷²Ge level scheme, and several corrections were made to t he previously-known level scheme. Transitions were characterised using Directional Correlation from Oriented states (DCO) and polarisation measurements, and spin-parity assignments were made for 7 new levels. Spin and parity were also reassigned to a previously-known structure. One of the new bands was ident ified as part of an octupole-vibrational band. No tetrahedral states were observed in the scheme despite N = 40 and Z = 32 having been identified as tetrahedral magic numbers. The meaning of the new 14⁺ yrast level for the underlying structure of the ground state band is discussed
- Full Text:
- Date Issued: 2012
Incorporating prey demographics and predator social structure into prey selection and carrying capacity estimates for cheetah
- Authors: Clements, Hayley Susan
- Date: 2012
- Subjects: Carnivorous animals , Cheetah
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10754 , http://hdl.handle.net/10948/d1018606
- Description: There is a need for a refined understanding of large carnivore prey preference and carrying capacity (K). To date, K estimates for large carnivores have been developed from predictions of carnivore diet at a prey and predator species-level. These predictions therefore assume that all social classes within a carnivore species display similar prey preferences and that all demographic classes within a prey species are equally preferred or avoided. The objective of this study was to investigate the importance of including prey demographics and carnivore social class in carnivore diet descriptions and thereby K estimates, using cheetah Acinonyx jubatus as a study species. It was predicted that prey sex, prey age and cheetah social class influence cheetah prey preferences, when they influence the risk and ease of prey capture, and that their inclusion in a K model would improve its predictive strength. Based on an analysis of 1290 kills from South Africa, male coalition cheetah were found to prefer a broader weight range of prey than solitary cheetah. Prey demographics further influenced cheetah prey preference, when it corresponded to differences in prey size and the presence of horns. The current species-level K regression model for cheetah is based on preferred prey and thus omits highly abundant antelope that often comprise the majority of the diet, an artefact of the way in which preferences are calculated. A refinement of the species-level K regression model, to account for prey demographic- and cheetah social class-level differences in diet and the biomass of accessible prey (defined in this study as all non-avoided prey) instead of just preferred prey, doubled the predictive strength of the K model. Because group-hunting enabled predation on a broader weight range of prey, cheetah K was influenced by the ratio of male coalition cheetah to solitary cheetah in the population. The refined K regression model is derived from ecosystems supporting an intact carnivore guild. A mechanistic approach to estimating K, based on Caughley‟s (1977) maximum sustainable yield model, therefore better predicted cheetah K in systems devoid of lion Panthera leo and African wild dog Lycaon pictus, which were found to suppress cheetah density. This study improves our understanding of the relationships between prey demographics, cheetah social classes and intra-guild competition in determining cheetah prey preferences and K. This study therefore paves the way for similar work on other large carnivores.
- Full Text:
- Date Issued: 2012
- Authors: Clements, Hayley Susan
- Date: 2012
- Subjects: Carnivorous animals , Cheetah
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10754 , http://hdl.handle.net/10948/d1018606
- Description: There is a need for a refined understanding of large carnivore prey preference and carrying capacity (K). To date, K estimates for large carnivores have been developed from predictions of carnivore diet at a prey and predator species-level. These predictions therefore assume that all social classes within a carnivore species display similar prey preferences and that all demographic classes within a prey species are equally preferred or avoided. The objective of this study was to investigate the importance of including prey demographics and carnivore social class in carnivore diet descriptions and thereby K estimates, using cheetah Acinonyx jubatus as a study species. It was predicted that prey sex, prey age and cheetah social class influence cheetah prey preferences, when they influence the risk and ease of prey capture, and that their inclusion in a K model would improve its predictive strength. Based on an analysis of 1290 kills from South Africa, male coalition cheetah were found to prefer a broader weight range of prey than solitary cheetah. Prey demographics further influenced cheetah prey preference, when it corresponded to differences in prey size and the presence of horns. The current species-level K regression model for cheetah is based on preferred prey and thus omits highly abundant antelope that often comprise the majority of the diet, an artefact of the way in which preferences are calculated. A refinement of the species-level K regression model, to account for prey demographic- and cheetah social class-level differences in diet and the biomass of accessible prey (defined in this study as all non-avoided prey) instead of just preferred prey, doubled the predictive strength of the K model. Because group-hunting enabled predation on a broader weight range of prey, cheetah K was influenced by the ratio of male coalition cheetah to solitary cheetah in the population. The refined K regression model is derived from ecosystems supporting an intact carnivore guild. A mechanistic approach to estimating K, based on Caughley‟s (1977) maximum sustainable yield model, therefore better predicted cheetah K in systems devoid of lion Panthera leo and African wild dog Lycaon pictus, which were found to suppress cheetah density. This study improves our understanding of the relationships between prey demographics, cheetah social classes and intra-guild competition in determining cheetah prey preferences and K. This study therefore paves the way for similar work on other large carnivores.
- Full Text:
- Date Issued: 2012
Investigating tools and techniques for improving software performance on multiprocessor computer systems
- Authors: Tristram, Waide Barrington
- Date: 2012
- Subjects: Multiprocessors , Multiprogramming (Electronic computers) , Parallel programming (Computer science) , Linux , Abstract data types (Computer science) , Threads (Computer programs) , Computer programming
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4655 , http://hdl.handle.net/10962/d1006651 , Multiprocessors , Multiprogramming (Electronic computers) , Parallel programming (Computer science) , Linux , Abstract data types (Computer science) , Threads (Computer programs) , Computer programming
- Description: The availability of modern commodity multicore processors and multiprocessor computer systems has resulted in the widespread adoption of parallel computers in a variety of environments, ranging from the home to workstation and server environments in particular. Unfortunately, parallel programming is harder and requires more expertise than the traditional sequential programming model. The variety of tools and parallel programming models available to the programmer further complicates the issue. The primary goal of this research was to identify and describe a selection of parallel programming tools and techniques to aid novice parallel programmers in the process of developing efficient parallel C/C++ programs for the Linux platform. This was achieved by highlighting and describing the key concepts and hardware factors that affect parallel programming, providing a brief survey of commonly available software development tools and parallel programming models and libraries, and presenting structured approaches to software performance tuning and parallel programming. Finally, the performance of several parallel programming models and libraries was investigated, along with the programming effort required to implement solutions using the respective models. A quantitative research methodology was applied to the investigation of the performance and programming effort associated with the selected parallel programming models and libraries, which included automatic parallelisation by the compiler, Boost Threads, Cilk Plus, OpenMP, POSIX threads (Pthreads), and Threading Building Blocks (TBB). Additionally, the performance of the GNU C/C++ and Intel C/C++ compilers was examined. The results revealed that the choice of parallel programming model or library is dependent on the type of problem being solved and that there is no overall best choice for all classes of problem. However, the results also indicate that parallel programming models with higher levels of abstraction require less programming effort and provide similar performance compared to explicit threading models. The principle conclusion was that the problem analysis and parallel design are an important factor in the selection of the parallel programming model and tools, but that models with higher levels of abstractions, such as OpenMP and Threading Building Blocks, are favoured.
- Full Text:
- Date Issued: 2012
- Authors: Tristram, Waide Barrington
- Date: 2012
- Subjects: Multiprocessors , Multiprogramming (Electronic computers) , Parallel programming (Computer science) , Linux , Abstract data types (Computer science) , Threads (Computer programs) , Computer programming
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4655 , http://hdl.handle.net/10962/d1006651 , Multiprocessors , Multiprogramming (Electronic computers) , Parallel programming (Computer science) , Linux , Abstract data types (Computer science) , Threads (Computer programs) , Computer programming
- Description: The availability of modern commodity multicore processors and multiprocessor computer systems has resulted in the widespread adoption of parallel computers in a variety of environments, ranging from the home to workstation and server environments in particular. Unfortunately, parallel programming is harder and requires more expertise than the traditional sequential programming model. The variety of tools and parallel programming models available to the programmer further complicates the issue. The primary goal of this research was to identify and describe a selection of parallel programming tools and techniques to aid novice parallel programmers in the process of developing efficient parallel C/C++ programs for the Linux platform. This was achieved by highlighting and describing the key concepts and hardware factors that affect parallel programming, providing a brief survey of commonly available software development tools and parallel programming models and libraries, and presenting structured approaches to software performance tuning and parallel programming. Finally, the performance of several parallel programming models and libraries was investigated, along with the programming effort required to implement solutions using the respective models. A quantitative research methodology was applied to the investigation of the performance and programming effort associated with the selected parallel programming models and libraries, which included automatic parallelisation by the compiler, Boost Threads, Cilk Plus, OpenMP, POSIX threads (Pthreads), and Threading Building Blocks (TBB). Additionally, the performance of the GNU C/C++ and Intel C/C++ compilers was examined. The results revealed that the choice of parallel programming model or library is dependent on the type of problem being solved and that there is no overall best choice for all classes of problem. However, the results also indicate that parallel programming models with higher levels of abstraction require less programming effort and provide similar performance compared to explicit threading models. The principle conclusion was that the problem analysis and parallel design are an important factor in the selection of the parallel programming model and tools, but that models with higher levels of abstractions, such as OpenMP and Threading Building Blocks, are favoured.
- Full Text:
- Date Issued: 2012
Investigation of the effect of short duration breaks in delaying the onset of performance related fatigue during long distance monotonous driving at different times of the day
- Authors: Ndaki, Ntombikayise
- Date: 2012
- Subjects: Fatigue -- Physiological aspects , Drowsiness -- Physiological aspects , Automobile driving -- Physiological aspects , Traffic safety -- South Africa , Automobile driving -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5170 , http://hdl.handle.net/10962/d1016353
- Description: Road traffic accidents are a serious burden to the health systems of many countries especially in South Africa. Research aimed at reducing traffic related accidents is of importance as traffic crashes are rated as the second leading cause of fatalities in South Africa and ninth in the world. Despite the extensive efforts into research and development of new technology, driver fatigue still remains a cause of vehicle accidents worldwide. Fatigue plays a role in up to 20% of vehicle accidents with many being serious or fatal. Numerous coping behaviours are employed by drivers to counteract the negative effects of fatigue. The most common coping behaviours include taking short naps, talking to passengers, listening to the radio, opening windows and drinking stimulants. Driving breaks have long been identified as an effective countermeasure against fatigue. Most research done in driving breaks has investigated the duration of the breaks, activity undertaken during the break and the frequency of the breaks taken outside the vehicle. However limited literature is available on the effectiveness of breaks in counteracting the effects of fatigue. The objective of the current study was aimed at assessing whether short duration breaks are an effective countermeasure against fatigue. Physiological, neurophysiological, subjective and performance measures were used as indicators for fatigue. Additional focus of the research was determining whether breaks were more or less effective at counteracting the effects of fatigue at different times of day. Twelve participants were recruited for the study, six males and six females. The participants were required to perform a driving task on a simulator for 90 minutes. The study consisted of four independent conditions, namely driving during the day with breaks, driving during the day without breaks, driving during the night with breaks and driving during the night without breaks. The without breaks conditions were similar except that they occurred at different times of the day, one session at night and the other session during day time, as was the case for the conditions with breaks. The driving task used in the current study was a low fidelity simulator tracking task. The participants were required to follow a centre line displayed on a tracking path as accurately as possible. The measurements that were recorded in this study included physiological, performance, subjective and neurophysiological. Physiological measures included heart rate and heart rate variability (frequency domain) and core body temperature. The ascending threshold of the critical flicker fusion frequency was the only neurophysiological measurement included in the current investigation. Performance was quantified by mean deviation from a centre line participants were meant to track. Two rating scales were used: Karolinska sleepiness scale and the Wits sleepiness scale were used for the measurement of subjective sleepiness. Heart rate, heart rate variability and mean deviation were measured continuously throughout the 90 minute driving task. Critical flicker fusion frequency, temperature and the subjective scales were measured before and after the 90 minute driving task. The results indicated that the short duration breaks during day time had a positive effect on driving performance; however the breaks at night had a negative effect on driving performance. Heart rate was higher during the day compared to night time and the heart rate variability high frequency spectrum values were lower during the day condition, to show the activation of the sympathetic nervous system which is characteristic of day time. The night conditions had lower heart rate values and higher heart rate variability high frequency values, which show the activation of the parasympathetic nervous system which is dominant during periods of fatigue and night time. Subjective sleepiness levels were also higher at night compared to day time.
- Full Text:
- Date Issued: 2012
- Authors: Ndaki, Ntombikayise
- Date: 2012
- Subjects: Fatigue -- Physiological aspects , Drowsiness -- Physiological aspects , Automobile driving -- Physiological aspects , Traffic safety -- South Africa , Automobile driving -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5170 , http://hdl.handle.net/10962/d1016353
- Description: Road traffic accidents are a serious burden to the health systems of many countries especially in South Africa. Research aimed at reducing traffic related accidents is of importance as traffic crashes are rated as the second leading cause of fatalities in South Africa and ninth in the world. Despite the extensive efforts into research and development of new technology, driver fatigue still remains a cause of vehicle accidents worldwide. Fatigue plays a role in up to 20% of vehicle accidents with many being serious or fatal. Numerous coping behaviours are employed by drivers to counteract the negative effects of fatigue. The most common coping behaviours include taking short naps, talking to passengers, listening to the radio, opening windows and drinking stimulants. Driving breaks have long been identified as an effective countermeasure against fatigue. Most research done in driving breaks has investigated the duration of the breaks, activity undertaken during the break and the frequency of the breaks taken outside the vehicle. However limited literature is available on the effectiveness of breaks in counteracting the effects of fatigue. The objective of the current study was aimed at assessing whether short duration breaks are an effective countermeasure against fatigue. Physiological, neurophysiological, subjective and performance measures were used as indicators for fatigue. Additional focus of the research was determining whether breaks were more or less effective at counteracting the effects of fatigue at different times of day. Twelve participants were recruited for the study, six males and six females. The participants were required to perform a driving task on a simulator for 90 minutes. The study consisted of four independent conditions, namely driving during the day with breaks, driving during the day without breaks, driving during the night with breaks and driving during the night without breaks. The without breaks conditions were similar except that they occurred at different times of the day, one session at night and the other session during day time, as was the case for the conditions with breaks. The driving task used in the current study was a low fidelity simulator tracking task. The participants were required to follow a centre line displayed on a tracking path as accurately as possible. The measurements that were recorded in this study included physiological, performance, subjective and neurophysiological. Physiological measures included heart rate and heart rate variability (frequency domain) and core body temperature. The ascending threshold of the critical flicker fusion frequency was the only neurophysiological measurement included in the current investigation. Performance was quantified by mean deviation from a centre line participants were meant to track. Two rating scales were used: Karolinska sleepiness scale and the Wits sleepiness scale were used for the measurement of subjective sleepiness. Heart rate, heart rate variability and mean deviation were measured continuously throughout the 90 minute driving task. Critical flicker fusion frequency, temperature and the subjective scales were measured before and after the 90 minute driving task. The results indicated that the short duration breaks during day time had a positive effect on driving performance; however the breaks at night had a negative effect on driving performance. Heart rate was higher during the day compared to night time and the heart rate variability high frequency spectrum values were lower during the day condition, to show the activation of the sympathetic nervous system which is characteristic of day time. The night conditions had lower heart rate values and higher heart rate variability high frequency values, which show the activation of the parasympathetic nervous system which is dominant during periods of fatigue and night time. Subjective sleepiness levels were also higher at night compared to day time.
- Full Text:
- Date Issued: 2012
Investigation of the performance of photovoltaic systems
- Authors: Alistoun, Warren James
- Date: 2012
- Subjects: Photovoltaic power systems -- South Africa , Photovoltaic power generation -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10517 , http://hdl.handle.net/10948/d1008396 , Photovoltaic power systems -- South Africa , Photovoltaic power generation -- South Africa
- Description: The main objective of this study was to investigate the performance of grid integrated PV systems. A data acquisition (DAQ) system was developed to monitor the performance of an existing grid integrated PV system with battery storage. This system is referred to as a grid assisted PV system. A data logger was used together with the inverters built in data logger to monitor environmental and electrical data on a grid tie PV system which was deployed during this study. To investigate the performance of these grid integrated PV systems PV and BOS device characterization was performed. This was achieved by using current voltage curve tracers and the DAQ system developed. Energy yield estimations were calculated referring to the literature review and a meteorological reference for comparison with measured energy yields from the grid tie PV system.
- Full Text:
- Date Issued: 2012
- Authors: Alistoun, Warren James
- Date: 2012
- Subjects: Photovoltaic power systems -- South Africa , Photovoltaic power generation -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10517 , http://hdl.handle.net/10948/d1008396 , Photovoltaic power systems -- South Africa , Photovoltaic power generation -- South Africa
- Description: The main objective of this study was to investigate the performance of grid integrated PV systems. A data acquisition (DAQ) system was developed to monitor the performance of an existing grid integrated PV system with battery storage. This system is referred to as a grid assisted PV system. A data logger was used together with the inverters built in data logger to monitor environmental and electrical data on a grid tie PV system which was deployed during this study. To investigate the performance of these grid integrated PV systems PV and BOS device characterization was performed. This was achieved by using current voltage curve tracers and the DAQ system developed. Energy yield estimations were calculated referring to the literature review and a meteorological reference for comparison with measured energy yields from the grid tie PV system.
- Full Text:
- Date Issued: 2012
Leopard population density, home range size and movement patterns in a mixed landuse area of the Mangwe District of Zimbabwe
- Authors: Grant, Tanith-Leigh
- Date: 2012
- Subjects: Leopard -- Monitoring -- Zimbabwe , Leopard -- Conservation -- Zimbabwe , Wildlife monitoring -- Zimbabwe , Wildlife conservation -- Zimbabwe
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5726 , http://hdl.handle.net/10962/d1005412 , Leopard -- Monitoring -- Zimbabwe , Leopard -- Conservation -- Zimbabwe , Wildlife monitoring -- Zimbabwe , Wildlife conservation -- Zimbabwe
- Description: Trophy hunting is often employed as a conservation management tool for large predators. However, in order for this method to succeed, hunting levels must be sustainable. Very little robust population data exist for African leopards (Panthera pardus) in general, and almost no density or spatial ecology data exist for leopards in Zimbabwe. Zimbabwe has one of the highest annual CITES leopard trophy hunting quotas in Africa, the sustainability of which has not been assessed, despite large scale landuse changes over the last 12 years. The focal area of this study was within the Mangwe district, in the south-west of Zimbabwe. The region is dominated by cattle and wildlife ranches, with high levels of leopard hunting, making it an important area for assessing leopard population density and spatial ecology. Three population density estimation methods were employed in my study: a spoor index survey, an unbaited camera-trapping survey and a baited camera-trapping survey. Using three calibration equations, spoor indices appeared to underestimate the leopard population (1.28-3.29 leopards/ 100 km²) as the equations were calibrated for areas with different habitats and leopard densities. In addition, the unbaited camera survey only produced six leopard photographs, unsuitable for individual identification and analysis. By contrast, the baited camera survey produced 292 identifiable leopard photographs, from which 13 individuals were identified. Density estimates calculated using the programme CAPTURE and the M[subscript]h model with the Mean Maximum Distance Moved Outside of Study Area (MMDMOSA) buffer method (4.79±0.83 leopards/100 km²), and the programme SPACECAP, using a buffer of 2.5 km (5.12±0.62 leopards/100 km²), appeared to generate the most reliable leopard population estimates. To assess the spatial ecology, three leopards (one male, two females) were captured and fitted with GPS collars. The home range estimates of the three leopards (95% Kernel UD: male 263 km², females 31 and 45 km²) were smaller than those of leopards in more arid regions, but larger than those of mesic habitats. This suggests that the Mangwe area has a higher quality habitat than the arid regions of Namibia, but less suitable habitat than protected bushveld areas (e.g. Kruger National Park, South Africa). My data represents the first robust leopard density and home range assessment for Zimbabwe. In addition, my results indicate that the current hunting quota issued to the Mangwe area is unsustainable. Consequently, I recommend revising the quota to five leopards for the entire area, and halving the current national leopard quota to 250, until a national leopard census is completed.
- Full Text:
- Date Issued: 2012
- Authors: Grant, Tanith-Leigh
- Date: 2012
- Subjects: Leopard -- Monitoring -- Zimbabwe , Leopard -- Conservation -- Zimbabwe , Wildlife monitoring -- Zimbabwe , Wildlife conservation -- Zimbabwe
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5726 , http://hdl.handle.net/10962/d1005412 , Leopard -- Monitoring -- Zimbabwe , Leopard -- Conservation -- Zimbabwe , Wildlife monitoring -- Zimbabwe , Wildlife conservation -- Zimbabwe
- Description: Trophy hunting is often employed as a conservation management tool for large predators. However, in order for this method to succeed, hunting levels must be sustainable. Very little robust population data exist for African leopards (Panthera pardus) in general, and almost no density or spatial ecology data exist for leopards in Zimbabwe. Zimbabwe has one of the highest annual CITES leopard trophy hunting quotas in Africa, the sustainability of which has not been assessed, despite large scale landuse changes over the last 12 years. The focal area of this study was within the Mangwe district, in the south-west of Zimbabwe. The region is dominated by cattle and wildlife ranches, with high levels of leopard hunting, making it an important area for assessing leopard population density and spatial ecology. Three population density estimation methods were employed in my study: a spoor index survey, an unbaited camera-trapping survey and a baited camera-trapping survey. Using three calibration equations, spoor indices appeared to underestimate the leopard population (1.28-3.29 leopards/ 100 km²) as the equations were calibrated for areas with different habitats and leopard densities. In addition, the unbaited camera survey only produced six leopard photographs, unsuitable for individual identification and analysis. By contrast, the baited camera survey produced 292 identifiable leopard photographs, from which 13 individuals were identified. Density estimates calculated using the programme CAPTURE and the M[subscript]h model with the Mean Maximum Distance Moved Outside of Study Area (MMDMOSA) buffer method (4.79±0.83 leopards/100 km²), and the programme SPACECAP, using a buffer of 2.5 km (5.12±0.62 leopards/100 km²), appeared to generate the most reliable leopard population estimates. To assess the spatial ecology, three leopards (one male, two females) were captured and fitted with GPS collars. The home range estimates of the three leopards (95% Kernel UD: male 263 km², females 31 and 45 km²) were smaller than those of leopards in more arid regions, but larger than those of mesic habitats. This suggests that the Mangwe area has a higher quality habitat than the arid regions of Namibia, but less suitable habitat than protected bushveld areas (e.g. Kruger National Park, South Africa). My data represents the first robust leopard density and home range assessment for Zimbabwe. In addition, my results indicate that the current hunting quota issued to the Mangwe area is unsustainable. Consequently, I recommend revising the quota to five leopards for the entire area, and halving the current national leopard quota to 250, until a national leopard census is completed.
- Full Text:
- Date Issued: 2012