Investigating the experiences of grade 8 English first additional language learners within an English home language classroom: a case study
- Authors: Abader, Naadirah
- Date: 2018
- Subjects: Language and languages -- Study and teaching -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , DEd
- Identifier: http://hdl.handle.net/10948/22662 , vital:30050
- Description: Teaching English home language (EHL) to a classroom of learners who are predominantly from another mother tongue context is a complex process because of the challenges faced by the learners. They struggle to cope with the demands of the EHL classroom, especially when their mother tongue is not recognized within the classroom context, as they move between different linguistic spaces. Despite their diverse identities, English language teachers continue to apply the linguistic approach of English only. This study was aimed at investigating the ways in which the experiences of non-mother tongue speakers in the EHL classroom affect the identities of these learners. Through a qualitative approach using photovoice with learners an attempt was made to probe their experiences of bringing other languages into an English home language classroom. Participants included 33 Grade 8 learners and their four English teachers from Taah High School in Nelson Mandela Bay. The findings have implications for the way in which diverse linguistic learners are taught in an EHL classroom, as the space that caged and rendered learner participants voiceless because they are not allowed to draw on their mother tongue linguistic repertoires, were changed. Learners mentioned that their teachers do not acknowledge the identity that each of them brings to the classroom.
- Full Text:
- Date Issued: 2018
- Authors: Abader, Naadirah
- Date: 2018
- Subjects: Language and languages -- Study and teaching -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , DEd
- Identifier: http://hdl.handle.net/10948/22662 , vital:30050
- Description: Teaching English home language (EHL) to a classroom of learners who are predominantly from another mother tongue context is a complex process because of the challenges faced by the learners. They struggle to cope with the demands of the EHL classroom, especially when their mother tongue is not recognized within the classroom context, as they move between different linguistic spaces. Despite their diverse identities, English language teachers continue to apply the linguistic approach of English only. This study was aimed at investigating the ways in which the experiences of non-mother tongue speakers in the EHL classroom affect the identities of these learners. Through a qualitative approach using photovoice with learners an attempt was made to probe their experiences of bringing other languages into an English home language classroom. Participants included 33 Grade 8 learners and their four English teachers from Taah High School in Nelson Mandela Bay. The findings have implications for the way in which diverse linguistic learners are taught in an EHL classroom, as the space that caged and rendered learner participants voiceless because they are not allowed to draw on their mother tongue linguistic repertoires, were changed. Learners mentioned that their teachers do not acknowledge the identity that each of them brings to the classroom.
- Full Text:
- Date Issued: 2018
Molecularly imprinted polymeric materials for adsorptive removal of nitrogen compounds from fuel oils
- Abdul-Quadir, Muhammad Sabiu
- Authors: Abdul-Quadir, Muhammad Sabiu
- Date: 2018
- Subjects: Polymerization , Organonitrogen compounds Nitrogen compounds
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/23426 , vital:30542
- Description: The deleterious effects of refractory polyaromatic hydrocarbons found in fuels such as organonitrogen compounds (quinoline, carbazole and its alkylated derivatives) are such that they emit NOx to the environment when combusted, thereby reducing air quality. These compounds also deactivate the catalyst used during fuel refinement and in catalytic converters of cars. Hydro-denitrogenation (HDN), a process currently being employed in petroleum refineries to eliminate organonitrogen compounds in fuels, is limited in treating these refractory compounds. Hence, this thesis describes the use of two separate complimentary approaches for the removal of organonitrogen compounds in fuel such as oxidative denitrogenation and adsorptive denitrogenation. The catalyzed oxidation of fuel oil model nitrogen containing compound, quinoline to quinoline N-oxide, was conducted under batch and continuous flow microreactor at 70°C by using tert-butylhydroperoxide (t-BuOOH) as oxidant and silica supported V2O5 as catalyst, followed by the selective adsorption of the quinoline N-oxide. An overall conversion of 62% quinoline N-oxide was observed. Quinoline-N-oxide in model fuel was absorbed by employing synthesized molecularly imprinted 2,6-pyridine-polybenzimidazole (2,6-PyPBI) nanofibers, 86% of quinoline-N-oxide was removed to give an adsorption capacity (qe) of 4.8 mg/g. DFT calculations to study the interactions of quinoline-N-oxide vs 2,6-PyPBI indicated that: (i) hydrogen bonding (through amino group of 2,6-PyPBI and oxygen atoms of the quinoline-N-oxide), (ii) pi-pi stacking and (iii) extensive number of van der Waals interactions took place. Several oxygenates from N-compounds were produced, thus, complicating the fuel matrix. Therefore, there is a need to move towards adsorptive denitrogenation. Poly-2-(1H-imidazol-2-yl)-4-phenol (PIMH) imprinted microspheres was prepared by suspension polymerization using 2-(2’-hydroxy-4-ethenylphenyl) imidazole as a functional monomer and ethylene glycol dimethacrylate as a crosslinker in the presence of various organonitrogen compounds (templates) to produce 2-(2’-hydroxy-4-ethenylphenyl) imidazole (PIMH). Imprinted microspheres show selectivity for various target model nitrogen-containing compounds with adsorption capacities of 6.8 ± 0.2 mg/g, 6.3 ± 0.3 mg/g and 5.8 ± 0.3 mg/g for quinoline, pyrimidine and carbazole, respectively. Adsorption selectivity increased in the order of quinoline (αi-r = 136.9) ˃ pyrimidine (αi-r = 126.2) ˃carbazole (αi-r = 86.3), when naphthalene was selected as a reference compound. Though, imprinted microspheres displayed excellent nitrogen compound removal both in model and real fuel, there was a need to improve the adsorbent adsorption capacity for N-compounds in fuel through the fabrication of imprinted nanofibers. Molecularly imprinted poly-2-(1H-imidazol-2-yl)-4-phenol nanofibers was prepared by electrospinning of 2-(2’-hydroxy-4-ethenylphenyl) imidazole (PIMH) in the presence of various organonitrogen compounds. These imprinted nanofibers show selectivity for various target model nitrogen-containing compounds with adsorption capacities of 11.7 ± 0.9 mg/g, 11.9 ± 0.8 mg/g and 11.3 ± 1.1 mg/g for quinoline, pyrimidine and carbazole, respectively. Adsorption selectivity increased in the order of pyrimidine (αi-r = 258.8) ˃ quinoline (αi-r = 235.5) ˃ carbazole (αi-r = 168.2). It further displayed excellent nitrogen removal in real fuel. The use of polybenzimidazole (PBI) nanofibers showed selective adsorption of organonitrogen compounds as imprinted sorbent also displayed high selectivity for their target model nitrogen-containing compounds with adsorption capacities of 11.4 ± 0.4 mg/g, 11.9 ± 0.2 mg/g and 10.9 ± 0.7 mg/g for quinoline, pyrimidine and carbazole respectively. Adsorption selectivity increased in the order of pyrimidine (αi-r = 241.5) ˃ quinoline (αi-r = 237.6) ˃ carbazole (αi-r = 170). Thermodynamic parameters obtained from isothermal titration calorimetry (ITC) revealed that quinoline-PIMH/PBI and pyrimidine-PIMH/PBI interactions are exothermic in nature, while carbazole-PIMH/PBI is endothermic in nature. DFT calculations indicated that π-π interactions/stacking and hydrogen bond interactions took place between N-compounds (carbazole, quinoline and pyrimidine) and adsorbent (PIMH and PBI). A significant reduction in the quantity of nitrogen containing compounds in hydrotreated fuel was observed (peak area reduction) when adsorbents (PIMH and PBI) was employed, however, the complex nature of organonitrogen compounds in fuel complicate the structure/function approach on MIPs for targeting these unwanted compounds.
- Full Text:
- Date Issued: 2018
- Authors: Abdul-Quadir, Muhammad Sabiu
- Date: 2018
- Subjects: Polymerization , Organonitrogen compounds Nitrogen compounds
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/23426 , vital:30542
- Description: The deleterious effects of refractory polyaromatic hydrocarbons found in fuels such as organonitrogen compounds (quinoline, carbazole and its alkylated derivatives) are such that they emit NOx to the environment when combusted, thereby reducing air quality. These compounds also deactivate the catalyst used during fuel refinement and in catalytic converters of cars. Hydro-denitrogenation (HDN), a process currently being employed in petroleum refineries to eliminate organonitrogen compounds in fuels, is limited in treating these refractory compounds. Hence, this thesis describes the use of two separate complimentary approaches for the removal of organonitrogen compounds in fuel such as oxidative denitrogenation and adsorptive denitrogenation. The catalyzed oxidation of fuel oil model nitrogen containing compound, quinoline to quinoline N-oxide, was conducted under batch and continuous flow microreactor at 70°C by using tert-butylhydroperoxide (t-BuOOH) as oxidant and silica supported V2O5 as catalyst, followed by the selective adsorption of the quinoline N-oxide. An overall conversion of 62% quinoline N-oxide was observed. Quinoline-N-oxide in model fuel was absorbed by employing synthesized molecularly imprinted 2,6-pyridine-polybenzimidazole (2,6-PyPBI) nanofibers, 86% of quinoline-N-oxide was removed to give an adsorption capacity (qe) of 4.8 mg/g. DFT calculations to study the interactions of quinoline-N-oxide vs 2,6-PyPBI indicated that: (i) hydrogen bonding (through amino group of 2,6-PyPBI and oxygen atoms of the quinoline-N-oxide), (ii) pi-pi stacking and (iii) extensive number of van der Waals interactions took place. Several oxygenates from N-compounds were produced, thus, complicating the fuel matrix. Therefore, there is a need to move towards adsorptive denitrogenation. Poly-2-(1H-imidazol-2-yl)-4-phenol (PIMH) imprinted microspheres was prepared by suspension polymerization using 2-(2’-hydroxy-4-ethenylphenyl) imidazole as a functional monomer and ethylene glycol dimethacrylate as a crosslinker in the presence of various organonitrogen compounds (templates) to produce 2-(2’-hydroxy-4-ethenylphenyl) imidazole (PIMH). Imprinted microspheres show selectivity for various target model nitrogen-containing compounds with adsorption capacities of 6.8 ± 0.2 mg/g, 6.3 ± 0.3 mg/g and 5.8 ± 0.3 mg/g for quinoline, pyrimidine and carbazole, respectively. Adsorption selectivity increased in the order of quinoline (αi-r = 136.9) ˃ pyrimidine (αi-r = 126.2) ˃carbazole (αi-r = 86.3), when naphthalene was selected as a reference compound. Though, imprinted microspheres displayed excellent nitrogen compound removal both in model and real fuel, there was a need to improve the adsorbent adsorption capacity for N-compounds in fuel through the fabrication of imprinted nanofibers. Molecularly imprinted poly-2-(1H-imidazol-2-yl)-4-phenol nanofibers was prepared by electrospinning of 2-(2’-hydroxy-4-ethenylphenyl) imidazole (PIMH) in the presence of various organonitrogen compounds. These imprinted nanofibers show selectivity for various target model nitrogen-containing compounds with adsorption capacities of 11.7 ± 0.9 mg/g, 11.9 ± 0.8 mg/g and 11.3 ± 1.1 mg/g for quinoline, pyrimidine and carbazole, respectively. Adsorption selectivity increased in the order of pyrimidine (αi-r = 258.8) ˃ quinoline (αi-r = 235.5) ˃ carbazole (αi-r = 168.2). It further displayed excellent nitrogen removal in real fuel. The use of polybenzimidazole (PBI) nanofibers showed selective adsorption of organonitrogen compounds as imprinted sorbent also displayed high selectivity for their target model nitrogen-containing compounds with adsorption capacities of 11.4 ± 0.4 mg/g, 11.9 ± 0.2 mg/g and 10.9 ± 0.7 mg/g for quinoline, pyrimidine and carbazole respectively. Adsorption selectivity increased in the order of pyrimidine (αi-r = 241.5) ˃ quinoline (αi-r = 237.6) ˃ carbazole (αi-r = 170). Thermodynamic parameters obtained from isothermal titration calorimetry (ITC) revealed that quinoline-PIMH/PBI and pyrimidine-PIMH/PBI interactions are exothermic in nature, while carbazole-PIMH/PBI is endothermic in nature. DFT calculations indicated that π-π interactions/stacking and hydrogen bond interactions took place between N-compounds (carbazole, quinoline and pyrimidine) and adsorbent (PIMH and PBI). A significant reduction in the quantity of nitrogen containing compounds in hydrotreated fuel was observed (peak area reduction) when adsorbents (PIMH and PBI) was employed, however, the complex nature of organonitrogen compounds in fuel complicate the structure/function approach on MIPs for targeting these unwanted compounds.
- Full Text:
- Date Issued: 2018
Pyridone substituted phthalocyanines: Photophysico-chemical properties and TD-DFT calculations
- Abdurrahmanoğlu, Şaziye, Canlica, Mevlüde, Mack, John, Nyokong, Tebello
- Authors: Abdurrahmanoğlu, Şaziye , Canlica, Mevlüde , Mack, John , Nyokong, Tebello
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/188216 , vital:44735 , xlink:href="https://doi.org/10.1142/S1088424617500730"
- Description: 4-(6-methyl-3-nitro-2-oxo-1,2-dihydropyridin-4-yloxy)phthalonitrile has been used to prepare a novel Zn(II) phthalocyanines with four peripheral pyridone substituents. The compound has been characterized by UV-visible absorption, FT-IR and 11H-NMR spectroscopy, elemental analysis and MALDI-TOF mass spectroscopy. The fluorescence, triplet quantum and singlet oxygen quantum yields have been determined and TD-DFT calculations have been used to identify trends in the electronic structure.
- Full Text:
- Date Issued: 2018
- Authors: Abdurrahmanoğlu, Şaziye , Canlica, Mevlüde , Mack, John , Nyokong, Tebello
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/188216 , vital:44735 , xlink:href="https://doi.org/10.1142/S1088424617500730"
- Description: 4-(6-methyl-3-nitro-2-oxo-1,2-dihydropyridin-4-yloxy)phthalonitrile has been used to prepare a novel Zn(II) phthalocyanines with four peripheral pyridone substituents. The compound has been characterized by UV-visible absorption, FT-IR and 11H-NMR spectroscopy, elemental analysis and MALDI-TOF mass spectroscopy. The fluorescence, triplet quantum and singlet oxygen quantum yields have been determined and TD-DFT calculations have been used to identify trends in the electronic structure.
- Full Text:
- Date Issued: 2018
Molecular studies on cholera and non-cholera causing vibrio pathogens isolated from some water resources and aquatic animals in Eastern Cape Province, South Africa
- Authors: Abioye, Oluwatayo Emmanuel
- Date: 2018
- Subjects: Vibrio cholerae -- Pathogens Vibrio cholerae
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9154 , vital:34283
- Description: The global socioeconomic and disease burdens of cholera; a disease caused by Vibrio cholerae have been ongoing since it was first reported in India in 1563. Unfortunately, emerging non-cholera causing Vibrio pathogens have been contributing to the malady of Vibrio genus globally in the recent years. The history of cholera outbreaks in South Africa have been well documented but information regarding other non-cholera Vibrio pathogens, is deficient in the literature. This study evaluated the statuses of aquatic animals including crustaceans, molluscs, and fish as important reservoirs of six key pathogenic Vibrio spp. in some important water resources and fish markets in the Eastern Cape, South Africa, between December 2016 and November 2017. Physiochemical parameters that are vital for water quality assessment were determined for all the water sampling points using standard methods. The parameters include pH, electrical conductivity (EC), total dissolved solids (TDS), salinity, temperature, total dissolved solids, turbidity and biological oxygen demand (BOD5). Vibrio spp. densities in water and aquatic animal samples were determined using membrane filtration and Most Probable NumberPolymerase chain reaction (MPN-PCR) methods respectively. Presumptive Vibrio spp. were recovered from the samples by direct plating of water samples and aliquots of aquatic animal homogenates on thiosulfate-citrate-bile salts-sucrose (TCBS) agar. Where necessary, samples were enriched using alkaline peptone water (APW) for optimum isolation of the targeted Vibrio spp. Presumptive isolates were identified and delineated into the six targeted pathogenic Vibrio spp. using PCR method. The antibiogram fingerprint of selected Vibrio spp. against a panel 18 panels of antibacterial agents were also carried out. Multiple antibiotic resistance phenotypes (MARPs) per set of isolates from water sampling sites and aquatic animal types were determined. Finally, multiple antibiotic resistance indices (MARI) for isolates recovered from the samples were determined. All experiments were done in replicate and data compared using one-way analysis of variance and one-tail t-test as appropriate. Correlation and regression analysis were also carried out as appropriate. Uncontrolled agricultural, industrial and domestic wastewater influxes, as well as run-off intrusions into the water resources investigated, were observed throughout the sampling season. The physicochemical qualities of the water resources varied with seasons. While pH observed for all the water sampling sites complied with South Africa and international standards, most sampling sites did not comply with set standards for one or more of the remaining physicochemical parameters. The parameters that frequently exhibited significant correlation and regression with Vibrio spp. densities were TDS, TSS, turbidity and temperature. The densities of Vibrio spp. in the samples studied varied with season and the highest density per sample types were observed in the summer. The prevalence of Vibrio spp. in freshwater, brackish water and aquatic animal samples were 79 percent, 78 percent and 86 percent respectively. The respective prevalence of V. cholerae, V. mimicus, V. fluvialis, V.alginolyticus, V. vulnificus and V. parahaemolyticus in freshwater samples (18 percent, 3 percent, ~1 percent, ~1 percent, <1 percent, < percent), brackish water(12 percent, 3 percent, 28 percent,~1 percent, ~1 percent, 9 percent) and aquatic animals (9 percent, 1 percent, 17 percent, 1 percent, 1 percent, 17 percent) were observed. All isolates from all the sample types showed very high antibiotic resistance against ampicillin and polymyxin B. Furthermore, MARPs were observed among isolates from the three sample types. Multiple antibiotic resistance indices (MARI) above 0.2 was observed at one site on Kowie River, three on Kubusi River and one on Buffalo River. The findings of this study suggest that most South Africa water-bodies especially freshwater resources are not well protected. This need to be addressed in a country like South Africa where availability of the freshwater resource for human activities is a critical issue. Base on the prevalence of pathogenic Vibrio spp. especially MARPs and sites that exhibited above 0.2 threshold for MARI, environmental base Vibrio species monitoring programs are recommended.
- Full Text:
- Date Issued: 2018
- Authors: Abioye, Oluwatayo Emmanuel
- Date: 2018
- Subjects: Vibrio cholerae -- Pathogens Vibrio cholerae
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9154 , vital:34283
- Description: The global socioeconomic and disease burdens of cholera; a disease caused by Vibrio cholerae have been ongoing since it was first reported in India in 1563. Unfortunately, emerging non-cholera causing Vibrio pathogens have been contributing to the malady of Vibrio genus globally in the recent years. The history of cholera outbreaks in South Africa have been well documented but information regarding other non-cholera Vibrio pathogens, is deficient in the literature. This study evaluated the statuses of aquatic animals including crustaceans, molluscs, and fish as important reservoirs of six key pathogenic Vibrio spp. in some important water resources and fish markets in the Eastern Cape, South Africa, between December 2016 and November 2017. Physiochemical parameters that are vital for water quality assessment were determined for all the water sampling points using standard methods. The parameters include pH, electrical conductivity (EC), total dissolved solids (TDS), salinity, temperature, total dissolved solids, turbidity and biological oxygen demand (BOD5). Vibrio spp. densities in water and aquatic animal samples were determined using membrane filtration and Most Probable NumberPolymerase chain reaction (MPN-PCR) methods respectively. Presumptive Vibrio spp. were recovered from the samples by direct plating of water samples and aliquots of aquatic animal homogenates on thiosulfate-citrate-bile salts-sucrose (TCBS) agar. Where necessary, samples were enriched using alkaline peptone water (APW) for optimum isolation of the targeted Vibrio spp. Presumptive isolates were identified and delineated into the six targeted pathogenic Vibrio spp. using PCR method. The antibiogram fingerprint of selected Vibrio spp. against a panel 18 panels of antibacterial agents were also carried out. Multiple antibiotic resistance phenotypes (MARPs) per set of isolates from water sampling sites and aquatic animal types were determined. Finally, multiple antibiotic resistance indices (MARI) for isolates recovered from the samples were determined. All experiments were done in replicate and data compared using one-way analysis of variance and one-tail t-test as appropriate. Correlation and regression analysis were also carried out as appropriate. Uncontrolled agricultural, industrial and domestic wastewater influxes, as well as run-off intrusions into the water resources investigated, were observed throughout the sampling season. The physicochemical qualities of the water resources varied with seasons. While pH observed for all the water sampling sites complied with South Africa and international standards, most sampling sites did not comply with set standards for one or more of the remaining physicochemical parameters. The parameters that frequently exhibited significant correlation and regression with Vibrio spp. densities were TDS, TSS, turbidity and temperature. The densities of Vibrio spp. in the samples studied varied with season and the highest density per sample types were observed in the summer. The prevalence of Vibrio spp. in freshwater, brackish water and aquatic animal samples were 79 percent, 78 percent and 86 percent respectively. The respective prevalence of V. cholerae, V. mimicus, V. fluvialis, V.alginolyticus, V. vulnificus and V. parahaemolyticus in freshwater samples (18 percent, 3 percent, ~1 percent, ~1 percent, <1 percent, < percent), brackish water(12 percent, 3 percent, 28 percent,~1 percent, ~1 percent, 9 percent) and aquatic animals (9 percent, 1 percent, 17 percent, 1 percent, 1 percent, 17 percent) were observed. All isolates from all the sample types showed very high antibiotic resistance against ampicillin and polymyxin B. Furthermore, MARPs were observed among isolates from the three sample types. Multiple antibiotic resistance indices (MARI) above 0.2 was observed at one site on Kowie River, three on Kubusi River and one on Buffalo River. The findings of this study suggest that most South Africa water-bodies especially freshwater resources are not well protected. This need to be addressed in a country like South Africa where availability of the freshwater resource for human activities is a critical issue. Base on the prevalence of pathogenic Vibrio spp. especially MARPs and sites that exhibited above 0.2 threshold for MARI, environmental base Vibrio species monitoring programs are recommended.
- Full Text:
- Date Issued: 2018
Rural-urban migration and its impact on rural development in Nigeria
- Authors: Abizu, Odion Stanley
- Date: 2018
- Subjects: Rural development Rural-urban migration -- Nigeria Rural poor
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/13815 , vital:39714
- Description: This thesis focuses on the developmental impact of rural-urban migration in Nigeria. While, recognising the negative impact of rural-urban migration, the study argues that in order to benefit internal migrants as well as rural development, there must be a paradigm shift to focus on how the benefits of migration on development can be achieved. It is proposed that rural-urban migration occurs as a response to economic, education, health, social and environmental factors. The challenges confronting rural areas are urban bias development paradigm pursued by the successive governments resulting in rural underdevelopment that exacerbates the rural urban disparity. And the neglect of agriculture in rural areas resulting from the discovery of oil in Nigeria. The study revealed that flawed policies adopted by successive Nigerian governments led to joblessness, entrenched social inequality and opportunities. One of the outcomes of these short-sighted policies was rural underdevelopment, which accelerated rural-urban migration in Nigeria. However, the study also demonstrates that the income flows from the migrants to the various rural households has contributed to improvement in the living standard of the households. The fragmentary approaches to rural development that is currently the case in Nigeria would not generate the required level of development and growth needed in the rural communities to improve the living standard. Thus, there is need for a policy framework in Nigeria, which would encourage the private sector to play a vital role in contributing their part in the provision of infrastructural facilities in the rural communities. Although this thesis is a case study of the impact of rural-urban migration on rural development in Nigeria, it can serve to appreciate the role migration can play in promoting development both in rural and urban areas in Nigeria and other Saharan Africa countries.
- Full Text:
- Date Issued: 2018
- Authors: Abizu, Odion Stanley
- Date: 2018
- Subjects: Rural development Rural-urban migration -- Nigeria Rural poor
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/13815 , vital:39714
- Description: This thesis focuses on the developmental impact of rural-urban migration in Nigeria. While, recognising the negative impact of rural-urban migration, the study argues that in order to benefit internal migrants as well as rural development, there must be a paradigm shift to focus on how the benefits of migration on development can be achieved. It is proposed that rural-urban migration occurs as a response to economic, education, health, social and environmental factors. The challenges confronting rural areas are urban bias development paradigm pursued by the successive governments resulting in rural underdevelopment that exacerbates the rural urban disparity. And the neglect of agriculture in rural areas resulting from the discovery of oil in Nigeria. The study revealed that flawed policies adopted by successive Nigerian governments led to joblessness, entrenched social inequality and opportunities. One of the outcomes of these short-sighted policies was rural underdevelopment, which accelerated rural-urban migration in Nigeria. However, the study also demonstrates that the income flows from the migrants to the various rural households has contributed to improvement in the living standard of the households. The fragmentary approaches to rural development that is currently the case in Nigeria would not generate the required level of development and growth needed in the rural communities to improve the living standard. Thus, there is need for a policy framework in Nigeria, which would encourage the private sector to play a vital role in contributing their part in the provision of infrastructural facilities in the rural communities. Although this thesis is a case study of the impact of rural-urban migration on rural development in Nigeria, it can serve to appreciate the role migration can play in promoting development both in rural and urban areas in Nigeria and other Saharan Africa countries.
- Full Text:
- Date Issued: 2018
Decentralization and quality assurance in the Ugandan primary education sector
- Authors: Abu-Baker, Mutaaya Sirajee
- Date: 2018
- Subjects: Schools -- Decentralization -- Uganda , Education, Primary -- Uganda , Educational change -- Uganda
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/57390 , vital:26897
- Description: The study presented in this thesis is a case study analysis of decentralization and quality assurance in a decentralized set up of the Ugandan Primary Schooling. The research looked at how the monitoring and evaluation informed the policy formulation process to regulate quality assurance in a decentralized governance of primary education. The Study was positioned in the critical realist paradigm, interpretive in orientation and used both coding and thematic techniques to understand the teachers’, SMC members’, and officers’ (at district and ministry levels) experiences and perceptions of quality assurance in a decentralized set up. Data was gathered using interviews, document analysis and observation methods. The findings indicated that the study was affected by eleven themes: Management System and Leadership, Human Resource Management, Finance Administration and Management, Parenting and Nutrition, Politics, Motivation, Social Structures and Patterns, Legislative Process and Policies, Infrastructure Development and Management, Community Involvement in Education and Curriculum and Professionalism. The monitoring and evaluation system had a framework in which it operates, though there was no quality assurance policy to guide the provision of quality education. The study finally indicated that there are more threats in a decentralized set up that put Quality in danger. Secondly, there was absence of supervision/inspection in schools as there was no evidence to prove this due to absence of reports. However, document analysis indicated visits of officers to schools. Records management was a problem to schools. Decentralization was adopted at different levels by different countries to address specific problems identified in view of service delivery. Finally, though monitoring and evaluation results informed the policy and decision makers, there was no quality assurance policy to guide the provision of quality education in institutions.
- Full Text:
- Date Issued: 2018
- Authors: Abu-Baker, Mutaaya Sirajee
- Date: 2018
- Subjects: Schools -- Decentralization -- Uganda , Education, Primary -- Uganda , Educational change -- Uganda
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/57390 , vital:26897
- Description: The study presented in this thesis is a case study analysis of decentralization and quality assurance in a decentralized set up of the Ugandan Primary Schooling. The research looked at how the monitoring and evaluation informed the policy formulation process to regulate quality assurance in a decentralized governance of primary education. The Study was positioned in the critical realist paradigm, interpretive in orientation and used both coding and thematic techniques to understand the teachers’, SMC members’, and officers’ (at district and ministry levels) experiences and perceptions of quality assurance in a decentralized set up. Data was gathered using interviews, document analysis and observation methods. The findings indicated that the study was affected by eleven themes: Management System and Leadership, Human Resource Management, Finance Administration and Management, Parenting and Nutrition, Politics, Motivation, Social Structures and Patterns, Legislative Process and Policies, Infrastructure Development and Management, Community Involvement in Education and Curriculum and Professionalism. The monitoring and evaluation system had a framework in which it operates, though there was no quality assurance policy to guide the provision of quality education. The study finally indicated that there are more threats in a decentralized set up that put Quality in danger. Secondly, there was absence of supervision/inspection in schools as there was no evidence to prove this due to absence of reports. However, document analysis indicated visits of officers to schools. Records management was a problem to schools. Decentralization was adopted at different levels by different countries to address specific problems identified in view of service delivery. Finally, though monitoring and evaluation results informed the policy and decision makers, there was no quality assurance policy to guide the provision of quality education in institutions.
- Full Text:
- Date Issued: 2018
Graphene quantum dots and their metallophthalocyanines nanoconjugates as novel photoluminescent nanosensors
- Authors: Achadu, Ojodomo John
- Date: 2018
- Subjects: Quantum dots , Graphene , Phthalocyanines , Nanoconjugates , Novel photoluminescent nanosensors , Metallophthalocyanines
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/60719 , vital:27821
- Description: The fabrication and application of graphene quantum dots (GQDs)-based photoluminescent probes for the detection of analytes is presented. GQDs were functionalized with complexes such as metallophthalocyanines (MPcs), metal nanoparticles (Au@Ag NPs), 2,2,6,6-tetramethyl(piperidin-1-yl)oxyl (TEMPO), maleimide and thymine for the sensing of target analytes such as ascorbic acid (AA), biothiols (cysteine, homocysteine and glutathione) and mercury ion (Hg²+). The design strategy and approach was based on the quenching of the fluorescence of the GQDs upon functionalization with the above-mentioned complexes, which could be restored in the presence of the target analytes (due to their specific interaction affinity with the complexes). For the detection of AA, GQDs were covalently and/or non-covalently conjugated to TEMPO-bearing complexes to form GQDs-4A-TEMPO and GQDs-TEMPO-MPc systems with nanomolar limits of detection. For the detection of biothiols, Au@Ag NPs and maleimide-bearing complexes (MPc), which have specific affinity to interact with biothiols, were deployed. Hg²+ detection involved the use of GQDs and/or MPcs with thiol and thymine groups, respectively. In addition, a smart sensing platform was designed for the dual detection of biothiols and Hg²+ using supramolecular hybrid of polyethyleneimine functionalized-GQDs and MPc-Au@Ag conjugate. The probe could detect, in a sequential manner, Hg²+ and biothiols with high sensitivity. Results obtained from the LODs of the probes showed that GQDs sensing performances could be enhanced in the presence of MPcs. The probes designed in this work were successfully deployed in the assays of the target analytes in real samples and the recoveries obtained confirmed the analytical applicability of the probes.
- Full Text:
- Date Issued: 2018
- Authors: Achadu, Ojodomo John
- Date: 2018
- Subjects: Quantum dots , Graphene , Phthalocyanines , Nanoconjugates , Novel photoluminescent nanosensors , Metallophthalocyanines
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/60719 , vital:27821
- Description: The fabrication and application of graphene quantum dots (GQDs)-based photoluminescent probes for the detection of analytes is presented. GQDs were functionalized with complexes such as metallophthalocyanines (MPcs), metal nanoparticles (Au@Ag NPs), 2,2,6,6-tetramethyl(piperidin-1-yl)oxyl (TEMPO), maleimide and thymine for the sensing of target analytes such as ascorbic acid (AA), biothiols (cysteine, homocysteine and glutathione) and mercury ion (Hg²+). The design strategy and approach was based on the quenching of the fluorescence of the GQDs upon functionalization with the above-mentioned complexes, which could be restored in the presence of the target analytes (due to their specific interaction affinity with the complexes). For the detection of AA, GQDs were covalently and/or non-covalently conjugated to TEMPO-bearing complexes to form GQDs-4A-TEMPO and GQDs-TEMPO-MPc systems with nanomolar limits of detection. For the detection of biothiols, Au@Ag NPs and maleimide-bearing complexes (MPc), which have specific affinity to interact with biothiols, were deployed. Hg²+ detection involved the use of GQDs and/or MPcs with thiol and thymine groups, respectively. In addition, a smart sensing platform was designed for the dual detection of biothiols and Hg²+ using supramolecular hybrid of polyethyleneimine functionalized-GQDs and MPc-Au@Ag conjugate. The probe could detect, in a sequential manner, Hg²+ and biothiols with high sensitivity. Results obtained from the LODs of the probes showed that GQDs sensing performances could be enhanced in the presence of MPcs. The probes designed in this work were successfully deployed in the assays of the target analytes in real samples and the recoveries obtained confirmed the analytical applicability of the probes.
- Full Text:
- Date Issued: 2018
A framework for managing the impact of information communication technology on employee well-being
- Authors: Ackerman, Richard
- Date: 2018
- Subjects: Work environment -- Psychological aspects , Work -- Technological innovations Employees -- Mental health Job stress Flextime
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/21449 , vital:29523
- Description: Employees who work with Information Communication Technology (hereafter referred to as ICT) face a risk in a rise of portable work. This in turn provides a means for employees to be connected to work, even when at home. Flexible work solutions and ability to work any hour of the week creates non-standard work schedules, which in turn can lead to a condition of work-a-holism. In most cases, this phenomena is driven by the belief that being constantly available to work demonstrates a devotion to one’s career and employer. The higher demands placed on employees, are in most cases at the expense of employees’ health, work-life balance and overall well-being. The primary objective of this study is therefore to establish whether the use of ICT outside of normal working hours affects employee well-being, as measured in terms of work-life balance. The second objective of this study is to provide the Target Company within the automotive component supply industry located in Port Elizabeth, in the Republic of South Africa, with a framework for managing the impact of ICT on employee well-being. The research paper has been prompted by the Target Company having recently introduced flexible working solutions for its employees. Research on the topic of alternative forms of work schedules, has shown flexible working arrangements gives employees more choice and control over where, when and by how much they work. Flexibility allows employees to actively shape their working conditions which helps them solve the demands of integrating personal life and work commitments. Whether management should encourage or permit the use of ICT amongst its employees to extend beyond normal working hours however remains unclear. In order to answer the research questions, this study has applied a quantitative, positivistic and deductive research based methodology. The approach applied within this study included conducting an extensive literature review on the effects of ICT use after hours on employee well-being. Thereafter using literature as a foundation, a measuring instrument was constructed in the form of an online questionnaire. The questionnaire was emailed to respondents to collect primary data, in order to measure the dependent and independent variables in terms of the hypothesised relationships. Testing of the variables followed, in order to establish whether the use of ICT use after hours negatively impacts employee well-being as measured in terms of work-life balance and stress. Secondly, the study set out to establish whether flexible working conditions linked to ICT use after hours negatively impacts on employee work-life balance. Thirdly, the relationship between physiological aspects such as age, gender and ethnicity were interrogated to establish an employees’ ability to adapt to changing work conditions, brought about as a direct result of ICT. Lastly, this study set out to establish which ICT tool (email, cellular phone, smartphone or other) has the highest impact on employee wellness. The data collection process yielded a sample of 103 respondents in middle and senior management levels of the Target Company, with 97% being recipients of employer provided ICT tools. These respondents represented the employees who are most likely to be affected by the phenomena under investigation. The survey data was captured and analysed using descriptive statistical techniques. The interpretation revealed aspects which formed the basis for managerial recommendations in the form of a proposed framework to manage the impact of ICT on employee well-being. The findings of the study found that the use of ICT outside of normal working hours has a negative influence on employee well-being as measured in terms of work-life balance and stress. The literature review findings were confirmed as supporting the statement that flexible working conditions linked to ICT use after hours having a positive influence on employee work-life balance. A further finding of the study was the confirmation of physiological aspects affects an employees’ ability to adapt to changing working conditions, thereby having a negative influence on employee work-life balance. Lastly, to the question of which ICT tool has the biggest impact on well-being, it was found that differing ICT tools (email, cellular, smartphone, or other) do not have a negative influence on employee wellness. These findings formed the basis for the recommendations in the form of a framework which has been proposed to manage the impact of ICT on employee well-being. The most pertinent aspect of the framework includes the employer communicating a well-defined ICT usage policy, which clearly outlines a standard operating procedure in relation to ICT use to all its employees. The findings furthermore propose that the employer should take the time to explain and train its employees as to what is considered acceptable and non-acceptable use of ICT devices, especially in relation to after hour use and leave absences from work. To ensure employee well-being, employees should be encouraged to implement defined time blocks for when they are available for work and when they are not. This can be applied through the act of switching off their ICT devices, which constitutes a boundary work tactic as an action in order to prevent work-related interruptions in the private sphere. While the employer can take described measures, it should also be noted that there is responsibility on the part of the employee to ensure personal wellness, including regular exercise, relaxation, and taking appropriate breaks when necessary. Moreover, employees can apply work and life boundary management techniques through the selective use of devices. By not applying automatic work email updates on ICT devices such as smart phones is yet another boundary management technique which can be utilised by employees. There are a range of practical suggestions which are proposed as a method to minimize the risk of ICT use on employee well-being within this research document. The above proposals have only served to highlight a select few aspects. While it is acknowledged that there are important advantages associated with the development and use of ICT, it is equally important to allow employees to recharge their batteries in the form of appropriate downtime to regain perspective, thus allowing the organisation to maintain its competitive advantage, with the well-being of its employees taken to heart.
- Full Text:
- Date Issued: 2018
- Authors: Ackerman, Richard
- Date: 2018
- Subjects: Work environment -- Psychological aspects , Work -- Technological innovations Employees -- Mental health Job stress Flextime
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/21449 , vital:29523
- Description: Employees who work with Information Communication Technology (hereafter referred to as ICT) face a risk in a rise of portable work. This in turn provides a means for employees to be connected to work, even when at home. Flexible work solutions and ability to work any hour of the week creates non-standard work schedules, which in turn can lead to a condition of work-a-holism. In most cases, this phenomena is driven by the belief that being constantly available to work demonstrates a devotion to one’s career and employer. The higher demands placed on employees, are in most cases at the expense of employees’ health, work-life balance and overall well-being. The primary objective of this study is therefore to establish whether the use of ICT outside of normal working hours affects employee well-being, as measured in terms of work-life balance. The second objective of this study is to provide the Target Company within the automotive component supply industry located in Port Elizabeth, in the Republic of South Africa, with a framework for managing the impact of ICT on employee well-being. The research paper has been prompted by the Target Company having recently introduced flexible working solutions for its employees. Research on the topic of alternative forms of work schedules, has shown flexible working arrangements gives employees more choice and control over where, when and by how much they work. Flexibility allows employees to actively shape their working conditions which helps them solve the demands of integrating personal life and work commitments. Whether management should encourage or permit the use of ICT amongst its employees to extend beyond normal working hours however remains unclear. In order to answer the research questions, this study has applied a quantitative, positivistic and deductive research based methodology. The approach applied within this study included conducting an extensive literature review on the effects of ICT use after hours on employee well-being. Thereafter using literature as a foundation, a measuring instrument was constructed in the form of an online questionnaire. The questionnaire was emailed to respondents to collect primary data, in order to measure the dependent and independent variables in terms of the hypothesised relationships. Testing of the variables followed, in order to establish whether the use of ICT use after hours negatively impacts employee well-being as measured in terms of work-life balance and stress. Secondly, the study set out to establish whether flexible working conditions linked to ICT use after hours negatively impacts on employee work-life balance. Thirdly, the relationship between physiological aspects such as age, gender and ethnicity were interrogated to establish an employees’ ability to adapt to changing work conditions, brought about as a direct result of ICT. Lastly, this study set out to establish which ICT tool (email, cellular phone, smartphone or other) has the highest impact on employee wellness. The data collection process yielded a sample of 103 respondents in middle and senior management levels of the Target Company, with 97% being recipients of employer provided ICT tools. These respondents represented the employees who are most likely to be affected by the phenomena under investigation. The survey data was captured and analysed using descriptive statistical techniques. The interpretation revealed aspects which formed the basis for managerial recommendations in the form of a proposed framework to manage the impact of ICT on employee well-being. The findings of the study found that the use of ICT outside of normal working hours has a negative influence on employee well-being as measured in terms of work-life balance and stress. The literature review findings were confirmed as supporting the statement that flexible working conditions linked to ICT use after hours having a positive influence on employee work-life balance. A further finding of the study was the confirmation of physiological aspects affects an employees’ ability to adapt to changing working conditions, thereby having a negative influence on employee work-life balance. Lastly, to the question of which ICT tool has the biggest impact on well-being, it was found that differing ICT tools (email, cellular, smartphone, or other) do not have a negative influence on employee wellness. These findings formed the basis for the recommendations in the form of a framework which has been proposed to manage the impact of ICT on employee well-being. The most pertinent aspect of the framework includes the employer communicating a well-defined ICT usage policy, which clearly outlines a standard operating procedure in relation to ICT use to all its employees. The findings furthermore propose that the employer should take the time to explain and train its employees as to what is considered acceptable and non-acceptable use of ICT devices, especially in relation to after hour use and leave absences from work. To ensure employee well-being, employees should be encouraged to implement defined time blocks for when they are available for work and when they are not. This can be applied through the act of switching off their ICT devices, which constitutes a boundary work tactic as an action in order to prevent work-related interruptions in the private sphere. While the employer can take described measures, it should also be noted that there is responsibility on the part of the employee to ensure personal wellness, including regular exercise, relaxation, and taking appropriate breaks when necessary. Moreover, employees can apply work and life boundary management techniques through the selective use of devices. By not applying automatic work email updates on ICT devices such as smart phones is yet another boundary management technique which can be utilised by employees. There are a range of practical suggestions which are proposed as a method to minimize the risk of ICT use on employee well-being within this research document. The above proposals have only served to highlight a select few aspects. While it is acknowledged that there are important advantages associated with the development and use of ICT, it is equally important to allow employees to recharge their batteries in the form of appropriate downtime to regain perspective, thus allowing the organisation to maintain its competitive advantage, with the well-being of its employees taken to heart.
- Full Text:
- Date Issued: 2018
The design of a juvenile detention centre for Port Elizabeth
- Authors: Adams, Eduan
- Date: 2018
- Subjects: Youth centers -- South Africa -- Port Elizabeth -- Designs and plans , Juvenile detention homes -- South Africa -- Port Elizabeth -- Designs and plans Juvenile delinquents -- Rehabilitation -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Masters , MArch
- Identifier: http://hdl.handle.net/10948/23969 , vital:30650
- Description: Out of a total of 236 active correctional centres across South Africa, only 5.5% of these cater to the needs of juvenile offenders, and none of these are located within the immediate Port Elizabeth area. This means that in their already fragile state, children from Port Elizabeth and the immediate surrounding areas are sent away from their families and support base to serve their sentence. Therefore, a need for a Juvenile Detention Centre within the Port Elizabeth area was realized. In this treatise an investigative study of existing Juvenile Detention Centres was carried out to which an understanding of the building typology, nature of the site and technical requirements was formulated. The site selected in Schauderville conforms to the site selection criteria established, and enhances the link to Nerina One Stop Child Justice Centre. The primary focus of the study was to design a building to provide secure residential care for juvenile offenders, offering a range of programmes to assist their rehabilitation back into society. The outcome, is presented as a set of architectural drawings and a model(s).
- Full Text:
- Date Issued: 2018
- Authors: Adams, Eduan
- Date: 2018
- Subjects: Youth centers -- South Africa -- Port Elizabeth -- Designs and plans , Juvenile detention homes -- South Africa -- Port Elizabeth -- Designs and plans Juvenile delinquents -- Rehabilitation -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Masters , MArch
- Identifier: http://hdl.handle.net/10948/23969 , vital:30650
- Description: Out of a total of 236 active correctional centres across South Africa, only 5.5% of these cater to the needs of juvenile offenders, and none of these are located within the immediate Port Elizabeth area. This means that in their already fragile state, children from Port Elizabeth and the immediate surrounding areas are sent away from their families and support base to serve their sentence. Therefore, a need for a Juvenile Detention Centre within the Port Elizabeth area was realized. In this treatise an investigative study of existing Juvenile Detention Centres was carried out to which an understanding of the building typology, nature of the site and technical requirements was formulated. The site selected in Schauderville conforms to the site selection criteria established, and enhances the link to Nerina One Stop Child Justice Centre. The primary focus of the study was to design a building to provide secure residential care for juvenile offenders, offering a range of programmes to assist their rehabilitation back into society. The outcome, is presented as a set of architectural drawings and a model(s).
- Full Text:
- Date Issued: 2018
Investigating long-term host-parasite dynamics in odontocetes in Southern Africa
- Authors: Adams, Inge Alison
- Date: 2018
- Subjects: Biological invasions -- Environmental aspects , Cetacea -- Indian Ocean , Ecological disturbances
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/23437 , vital:30543
- Description: Cetaceans are a very diverse family and globally, various aspects about their biology have been studied, including infection by parasites. Twenty-five dolphin species can be found in the South African subregion and very little information is available detailing which parasites are infecting them. Parasite samples have been collected opportunistically since the 1970’s from dolphins incidentally caught in shark nets off the KwaZulu-Natal coastline as well as from dolphins stranded along the Eastern Cape coastline. These samples have been stored in the Graham Ross Marine Mammal Collection at the Port Elizabeth Museum and samples belonging to eight odontocete species have been used in the present analysis. These eight species are: the Indian Ocean Humpback Dolphin (Sousa plumbea), the Indo-Pacific Bottlenose Dolphin (Tursiops aduncus), the Long-beaked Common Dolphin (Delphinus capensis), the Risso’s Dolphin (Grampus griseus), the Pantropical Spotted Dolphin (Stenella attenuata), the Striped Dolphin (Stenella coeruleoalba), the Pygmy Sperm Whale (Kogia breviceps) and the Dwarf Sperm Whale (Kogia breviceps). This study aims to investigate which parasites are infecting odontocetes in Southern Africa. Secondly, this study aims to investigate whether any trends in infection exist between sexes, age groups, cause of death and over time. Eighteen parasite species were found to infect odontocetes in Southern Africa. Two could only be identified to family level, seven to genus level and eight to species level. Only one parasite, a trematode, could not be identified and several parasite species were new host records for the subregion. Results showed that the prevalence of parasitic infection has increased over the last four decades, prompting questions regarding the health of our oceans. This study is the first to comprehensively investigate which parasite species are found in these eight odontocete species in Southern Africa. This baseline data will contribute greatly to other types of research involving parasites and provides us with information regarding the health of our ocean.
- Full Text:
- Date Issued: 2018
- Authors: Adams, Inge Alison
- Date: 2018
- Subjects: Biological invasions -- Environmental aspects , Cetacea -- Indian Ocean , Ecological disturbances
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/23437 , vital:30543
- Description: Cetaceans are a very diverse family and globally, various aspects about their biology have been studied, including infection by parasites. Twenty-five dolphin species can be found in the South African subregion and very little information is available detailing which parasites are infecting them. Parasite samples have been collected opportunistically since the 1970’s from dolphins incidentally caught in shark nets off the KwaZulu-Natal coastline as well as from dolphins stranded along the Eastern Cape coastline. These samples have been stored in the Graham Ross Marine Mammal Collection at the Port Elizabeth Museum and samples belonging to eight odontocete species have been used in the present analysis. These eight species are: the Indian Ocean Humpback Dolphin (Sousa plumbea), the Indo-Pacific Bottlenose Dolphin (Tursiops aduncus), the Long-beaked Common Dolphin (Delphinus capensis), the Risso’s Dolphin (Grampus griseus), the Pantropical Spotted Dolphin (Stenella attenuata), the Striped Dolphin (Stenella coeruleoalba), the Pygmy Sperm Whale (Kogia breviceps) and the Dwarf Sperm Whale (Kogia breviceps). This study aims to investigate which parasites are infecting odontocetes in Southern Africa. Secondly, this study aims to investigate whether any trends in infection exist between sexes, age groups, cause of death and over time. Eighteen parasite species were found to infect odontocetes in Southern Africa. Two could only be identified to family level, seven to genus level and eight to species level. Only one parasite, a trematode, could not be identified and several parasite species were new host records for the subregion. Results showed that the prevalence of parasitic infection has increased over the last four decades, prompting questions regarding the health of our oceans. This study is the first to comprehensively investigate which parasite species are found in these eight odontocete species in Southern Africa. This baseline data will contribute greatly to other types of research involving parasites and provides us with information regarding the health of our ocean.
- Full Text:
- Date Issued: 2018
Cloud computing adoption: Strategies for Sub-Saharan Africa SMEs for enhancing competitiveness
- Authors: Adane, Martin
- Date: 2018
- Subjects: business intelligence small businesses business planning Article
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/6862 , vital:51101 , xlink:href="https://doi.org/10.1080/20421338.2018.1439288"
- Description: Small and medium-sized enterprises (SMEs) need to design and implement strategies for adopting new technologies as a competitive tool for maintaining their competitive advantage and cloud computing is at its peak. Since its emergence, there has been a surge in the adoption of cloud computing with research into its adoption primarily concentrated on bigger firms. However, a major characteristic of cloud computing is the anticipated possibilities it holds for SMEs. This paper primarily explores cloud computing adoption strategies for SMEs operating in Sub-Saharan Africa. Using a mixed method approach, a sample of 261 respondents and 11 SMEs was respectively used for the quantitative and qualitative stages of the research. The results reveal that, even though SMEs have their unique expectations for adopting cloud computing, they were of the consensus that elements of an adoption strategy should include adoption goals, a roadmap, and considerations. The paper makes specific recommendations to SMEs to develop adoption strategies that meet their specific needs if they expect cloud computing to play a stronger role to support more strategic initiatives to grow their businesses and improve customer experience.
- Full Text:
- Date Issued: 2018
- Authors: Adane, Martin
- Date: 2018
- Subjects: business intelligence small businesses business planning Article
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/6862 , vital:51101 , xlink:href="https://doi.org/10.1080/20421338.2018.1439288"
- Description: Small and medium-sized enterprises (SMEs) need to design and implement strategies for adopting new technologies as a competitive tool for maintaining their competitive advantage and cloud computing is at its peak. Since its emergence, there has been a surge in the adoption of cloud computing with research into its adoption primarily concentrated on bigger firms. However, a major characteristic of cloud computing is the anticipated possibilities it holds for SMEs. This paper primarily explores cloud computing adoption strategies for SMEs operating in Sub-Saharan Africa. Using a mixed method approach, a sample of 261 respondents and 11 SMEs was respectively used for the quantitative and qualitative stages of the research. The results reveal that, even though SMEs have their unique expectations for adopting cloud computing, they were of the consensus that elements of an adoption strategy should include adoption goals, a roadmap, and considerations. The paper makes specific recommendations to SMEs to develop adoption strategies that meet their specific needs if they expect cloud computing to play a stronger role to support more strategic initiatives to grow their businesses and improve customer experience.
- Full Text:
- Date Issued: 2018
Strategies to facilitate the use of birth care provided by skilled birth attendants in the rural areas of Ghana
- Authors: Adatara, Peter
- Date: 2018
- Subjects: Maternity nursing -- Ghana , Neonatal nursing -- methods Delivery (Obstetrics) Midwifery -- Ghana
- Language: English
- Type: Thesis , Doctoral , DCur
- Identifier: http://hdl.handle.net/10948/23415 , vital:30541
- Description: Increasing skilled attendance during childbirth is well established in literature to play a significant role in averting the many preventable maternal deaths that occur in developing countries such as Ghana.Inadequate utilisation of skilled birth care services in Sub-Saharan Africa is believed to be a major hindrance to efforts aimed at improving the health of women, especially during delivery. There is low utilisation of birth care services provided by skilled birth attendants in the rural areas in Ghana. The purpose of this study was to develop strategies that could facilitate the utilisation of skilled birth care provided by skilled birth attendants in the rural areas of Ghana. The study adopted a qualitative, explorative, descriptive and contextual method to explore and describe women’s experiences regarding the utilisation or non-utilisation of skilled birth care services provided by skilled birth attendants in the rural areas in Ghana. The study was conducted in three phases: Phase One of this study described the research population, sampling method, data collection and analysis to obtain women’s experiences regarding the utilisation or non-utilisation of skilled birth care services. In this study, data were collected and analysed from the research population. The research population consisted of women who utilised skilled birth care attendants or unskilled care attendants during child birth. The data collection method used was individual interviews. Data collected from the interviews were transcribed verbatim and analysed according to the steps suggested by Tesch to identify themes and sub-themes. The study identified three themes and sub-themes: Theme 1: Experiences of participants related to the use of skilled birth care attendants; Theme 2: Participants expressed their reasons for choosing a home birth; Theme 3: Participants offered suggestions to improve skilled birth care at healthcare facilities. The findings in Phase one of this study formed the basis for the development of the strategies in Phase three. Phase Two of the research design focused on the development of a conceptual framework based on the research findings and utilized to develop strategies which skilled birth attendant may use to facilitate the use of skilled birth care services by women in the rural areas of Ghana where there is low utilisation of birth services provided by skilled birth attendants. The conceptual framework of Dickoff, James and Wiedenbach was used to provide a guide and a link between the different concepts in the research study. Phase Three of this study focused on the development of strategies that will facilitate an increased in the labouring women utilisation of the services provided by skilled birth attendants. The data obtained was used to developed three strategies such as collaboration, education and training, supportive supervision as well as community mobilisation and participation to guide skilled birth attendants to facilitate the utilisation of skilled birth care services during childbirth. The WHO Health for All model, the theoretical model underpinning this study, also guided the development of the strategies to facilitate labouring women utilisation of the services provided by skilled birth attendants. Recommendations regarding the implementation of a national collaborative, education and supportive supervision policies on a macro level are made, as well as recommendations for nursing practice, education and research. It is therefore concluded that the researcher succeeded in achieving the purpose for this study because the strategies which are understandable, clear, simple, applicable and significant to skilled birth care in rural areas has been developed for use by skilled birth attendants to facilitate the utilisation of skilled birth care provided by skilled birth attendants in the rural areas of Ghana.
- Full Text:
- Date Issued: 2018
- Authors: Adatara, Peter
- Date: 2018
- Subjects: Maternity nursing -- Ghana , Neonatal nursing -- methods Delivery (Obstetrics) Midwifery -- Ghana
- Language: English
- Type: Thesis , Doctoral , DCur
- Identifier: http://hdl.handle.net/10948/23415 , vital:30541
- Description: Increasing skilled attendance during childbirth is well established in literature to play a significant role in averting the many preventable maternal deaths that occur in developing countries such as Ghana.Inadequate utilisation of skilled birth care services in Sub-Saharan Africa is believed to be a major hindrance to efforts aimed at improving the health of women, especially during delivery. There is low utilisation of birth care services provided by skilled birth attendants in the rural areas in Ghana. The purpose of this study was to develop strategies that could facilitate the utilisation of skilled birth care provided by skilled birth attendants in the rural areas of Ghana. The study adopted a qualitative, explorative, descriptive and contextual method to explore and describe women’s experiences regarding the utilisation or non-utilisation of skilled birth care services provided by skilled birth attendants in the rural areas in Ghana. The study was conducted in three phases: Phase One of this study described the research population, sampling method, data collection and analysis to obtain women’s experiences regarding the utilisation or non-utilisation of skilled birth care services. In this study, data were collected and analysed from the research population. The research population consisted of women who utilised skilled birth care attendants or unskilled care attendants during child birth. The data collection method used was individual interviews. Data collected from the interviews were transcribed verbatim and analysed according to the steps suggested by Tesch to identify themes and sub-themes. The study identified three themes and sub-themes: Theme 1: Experiences of participants related to the use of skilled birth care attendants; Theme 2: Participants expressed their reasons for choosing a home birth; Theme 3: Participants offered suggestions to improve skilled birth care at healthcare facilities. The findings in Phase one of this study formed the basis for the development of the strategies in Phase three. Phase Two of the research design focused on the development of a conceptual framework based on the research findings and utilized to develop strategies which skilled birth attendant may use to facilitate the use of skilled birth care services by women in the rural areas of Ghana where there is low utilisation of birth services provided by skilled birth attendants. The conceptual framework of Dickoff, James and Wiedenbach was used to provide a guide and a link between the different concepts in the research study. Phase Three of this study focused on the development of strategies that will facilitate an increased in the labouring women utilisation of the services provided by skilled birth attendants. The data obtained was used to developed three strategies such as collaboration, education and training, supportive supervision as well as community mobilisation and participation to guide skilled birth attendants to facilitate the utilisation of skilled birth care services during childbirth. The WHO Health for All model, the theoretical model underpinning this study, also guided the development of the strategies to facilitate labouring women utilisation of the services provided by skilled birth attendants. Recommendations regarding the implementation of a national collaborative, education and supportive supervision policies on a macro level are made, as well as recommendations for nursing practice, education and research. It is therefore concluded that the researcher succeeded in achieving the purpose for this study because the strategies which are understandable, clear, simple, applicable and significant to skilled birth care in rural areas has been developed for use by skilled birth attendants to facilitate the utilisation of skilled birth care provided by skilled birth attendants in the rural areas of Ghana.
- Full Text:
- Date Issued: 2018
A multi-stakeholder approach to productivity improvement in the South African construction industry
- Authors: Adebowale, Oluseyi Julius
- Date: 2018
- Subjects: Labor productivity , Construction industry -- Labor productivity Construction industry -- South Africa Construction industry -- Management
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/19597 , vital:28902
- Description: As poor productivity in construction continues to constitute a setback to projects’ success in different countries of the world, the situation in South African construction is no exception. Poor productivity on construction projects and its detrimental impact on project objectives have continually resonated for decades. As a result, several strategies, interventions, and measures that aimed at improving productivity in the construction sector have been proposed. Nevertheless, the phenomenon importunately creates a major challenge on construction projects, and by extension, the construction industry especially in developing countries. Even when construction management literature acknowledged the commonalities in productivity influences, the practice of addressing construction productivity influences as separate entities has gained much relevance over the years. Taking into perspective, the multidisciplinary and complexity inherent in construction, a systemic approach to addressing construction productivity influences is fast becoming essential. This study addressed productivity-influencing factors by considering factors that are related to rework, shortage of construction resources, constructability, political, socio-economic, working environment, welfare conditions, disputes, planning and training. Consequently, the interdependencies of the most significant productivity-influencing factors in South African construction was developed in the form of a qualitative model. The study adopts the underlying assumptions of the quantitative approach, but also accepts some qualitative data. With regards to the subject of enquiry, the paradigm of the research is positivism which consists of realists’ ontological prescriptions and objectivists’ epistemological prescriptions. Site engineers, site managers, and site supervisors working for cidb registered contractors in four South African provinces constitute the sample stratum. Statistical Package for the Social Sciences (SPSS) version 24 was used to analyse the data gathered from these project participants; both descriptive and inferential statistics were used. Descriptive statistics was used to summarise and describe the data obtained, while inferential statistics in the form of analysis of variance, T-test, and Tukey post hoc test were adopted. The study reported the most significant factors which negatively influence productivity on South African construction projects as inadequate workers’ skills, defective workmanship, the practice of awarding contracts to lowest bidders, political activities resulting in industrial action, inadequate contractors’ experience in project planning, payment delays by contractors, and corruption. Furthermore, it was found that contractors largely share similar perceptions regarding the factors that constrain productivity growth in South African construction. The approximate level of labour, material, and plant productivity on South African construction projects is 50.4%, 72.9%, and 62.0% respectively, while the approximate multifactor productivity was estimated as 61.8%. The study concluded that productivity has a considerable relationship with time and cost and a less potent relationship with quality. Based on the research findings, contractors’, consultants’, clients’ and government qualitative intervention models were proposed while recommendations towards improving South African construction productivity were made. The study recommended the reinvention of the apprentice training system to develop skills in construction, the involvement of stakeholders in improving workers’ skills, improved collaboration between the construction industry and higher institutions of learning regarding curricula development, adequate supervision of construction activities to mitigate the propagation of rework on construction projects, character in leadership, and integrity of project stakeholders.
- Full Text:
- Date Issued: 2018
- Authors: Adebowale, Oluseyi Julius
- Date: 2018
- Subjects: Labor productivity , Construction industry -- Labor productivity Construction industry -- South Africa Construction industry -- Management
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/19597 , vital:28902
- Description: As poor productivity in construction continues to constitute a setback to projects’ success in different countries of the world, the situation in South African construction is no exception. Poor productivity on construction projects and its detrimental impact on project objectives have continually resonated for decades. As a result, several strategies, interventions, and measures that aimed at improving productivity in the construction sector have been proposed. Nevertheless, the phenomenon importunately creates a major challenge on construction projects, and by extension, the construction industry especially in developing countries. Even when construction management literature acknowledged the commonalities in productivity influences, the practice of addressing construction productivity influences as separate entities has gained much relevance over the years. Taking into perspective, the multidisciplinary and complexity inherent in construction, a systemic approach to addressing construction productivity influences is fast becoming essential. This study addressed productivity-influencing factors by considering factors that are related to rework, shortage of construction resources, constructability, political, socio-economic, working environment, welfare conditions, disputes, planning and training. Consequently, the interdependencies of the most significant productivity-influencing factors in South African construction was developed in the form of a qualitative model. The study adopts the underlying assumptions of the quantitative approach, but also accepts some qualitative data. With regards to the subject of enquiry, the paradigm of the research is positivism which consists of realists’ ontological prescriptions and objectivists’ epistemological prescriptions. Site engineers, site managers, and site supervisors working for cidb registered contractors in four South African provinces constitute the sample stratum. Statistical Package for the Social Sciences (SPSS) version 24 was used to analyse the data gathered from these project participants; both descriptive and inferential statistics were used. Descriptive statistics was used to summarise and describe the data obtained, while inferential statistics in the form of analysis of variance, T-test, and Tukey post hoc test were adopted. The study reported the most significant factors which negatively influence productivity on South African construction projects as inadequate workers’ skills, defective workmanship, the practice of awarding contracts to lowest bidders, political activities resulting in industrial action, inadequate contractors’ experience in project planning, payment delays by contractors, and corruption. Furthermore, it was found that contractors largely share similar perceptions regarding the factors that constrain productivity growth in South African construction. The approximate level of labour, material, and plant productivity on South African construction projects is 50.4%, 72.9%, and 62.0% respectively, while the approximate multifactor productivity was estimated as 61.8%. The study concluded that productivity has a considerable relationship with time and cost and a less potent relationship with quality. Based on the research findings, contractors’, consultants’, clients’ and government qualitative intervention models were proposed while recommendations towards improving South African construction productivity were made. The study recommended the reinvention of the apprentice training system to develop skills in construction, the involvement of stakeholders in improving workers’ skills, improved collaboration between the construction industry and higher institutions of learning regarding curricula development, adequate supervision of construction activities to mitigate the propagation of rework on construction projects, character in leadership, and integrity of project stakeholders.
- Full Text:
- Date Issued: 2018
Screening for cardiometabolic risk factors among commercial drivers in Buffalo City Metropolitan Municipality, Eastern Cape, South Africa
- Authors: Adedokun, Aanuoluwa Odunayo
- Date: 2018
- Subjects: Diabetes -- South Africa -- Eastern Cape Obesity -- South Africa -- Eastern Cape Commercial vehicles
- Language: English
- Type: Thesis , Masters , Nursing Science
- Identifier: http://hdl.handle.net/10353/10431 , vital:35463
- Description: Cardio-metabolic risk factors are the aggregates of conditions that increase the susceptibility of developing cardio-metabolic diseases such as cardiovascular diseases (CVD) and diabetes. This includes insulin resistance, obesity, hyperglyceamia, dyslipoproteinemia, hyperinsulinemia and hypertension. This condition are worsen by smoking and physical inactivity as they mostlyare not easily detected. This was a cross-sectional survey of 403 commercial taxi drivers at ten different taxi ranks in BCMM. The study utilized a convenient sampling technique for the participants of the study. The WHO STEPwise approach was used for data collection. The WHO STEPwise questionnaire was used for a face-to-face interview. Socio-demographic (sex, age, marital status, driving experience, income) and behavioural characteristics (smoking, alcohol consumption, consumption of sweet drinks, physical activity, and dietary intake) of participants were obtained. Also, objective reports concerning anthropometric measurements (weight, height, waist and hip circumference), blood pressure and biochemical measurement that is the blood glucose measurement. All anthropometric measurements were taken following a standard procedure using a Lufkin nonextensible flexible anthropometric tape (W606PM), Rosscraft, Canada and a SECA weighing scale and stadiometer (Hamburg, Germany). Blood pressure was checked also in accordance with standard protocols using a Medic+ Digital Blood Pressure Monitor Model 1219 (Hamburg, Germany). A validated ACCU-CHEK glucose monitoring apparatus (Mannheim, Germany) wasused for blood glucose measurement. Overweight and obesity was defined as a body mass index (BMI) of 25.0 kg/m2 –29.9 kg/m2 and≥ 30 kg/m2, respectively. Pre-hypertension was defined according JNC-8 criteria a systolic blood pressure of 120- 139 mmHg and diastolic of BP 80-89 mmHg while hypertension was defined as an average of two systolic BP ≥140mmHg and/or diastolic BP of ≥90mmHg or a history of hypertension or anti hypertensive medication use. Diabetes status was determined using the fasting blood glucose (FBG) test and defined as a FBG ≥ 7.0mmol/L or self-reports of history or current diabetes medication use (treatment), while pre-diabetes was defined as a FBG of 5.6-6.9mmol/L. Awareness of diabetes was defined as self-reported history of diabetes among the people with diabetes. Metabolic syndrome status was determined according to the National agreement among makers of abdominal obesity. Statistical Package for Social Sciences (SPSS) was used for data analysis. The mean age of the participants was 43.3 (SD12.5) years. The prevalence of overweight and obesity was 34.0 percent and 38.0 percent, respectively. Age, marital status, period of driving, not-smoking, hypertension and diabetes were significantly associated with obesity. In logistic regression analysis, after adjusting for confounding factors, only age (OR 1.6, CI 1.0-2.7), hypertension (OR 3.6, CI 2.3-5.7) and non-smoking (OR 2.0, CI 1.3-3.1) were the independent and significant determinants of obesity. The prevalence rates of abdominal obesity by waist circumference (WC), waist-hip-ratio (WHR), waist-to-height ratio (WHtR) and neck circumference (NC) were 61.5 percent, 67.5 percent, 80.1 percent and 65.3 percent, respectively. A strong correlations exists between WHR and WC (>0.64); and WHtR and WC (>0.62). There exists a good agreement between WC and WHR. Also, a moderate agreement exists between WC and WHtR, WC and NC, WHR and WHtR, and WHtR and NC. The prevalence of above normal body composition for participants with hypertension was 71.0 percent, 65.9 percent, 63.2 percent and 66.5 percent by WC, WHR, WHtR and NC, respectively. For diabetes, the prevalence was 20.6 percent, 19.4 percent, 17.0 percent and 18.6 percent by WC, WHR, WHtR and NC, respectively. Waist circumference was a stronger predictor of hypertension and diabetics, with odds ratio of 3.7 (95 percent CI: 2.3-6.1 and 3.1 (95 percent CI: 1.6-6.0), compared to NC, with odds ratio of 1.7 (95 percent CI: 1.1-2.8). The prevalence of pre-hypertension was 33.7 percent and hypertension was 57.0 percent. Age, marital status, level of education, period of driving, obesity, alcohol, sweet drinks consumption and diabetes were significantly associated with hypertension. After adjusting for confounders, age >35 years (P=0.004), obesity and alcohol use (P<0.001), period of driving >5years (p=0.028) and diabetes (P=0.003) were significant predictors of hypertension. Prevalence of pre-diabetes and diabetes was 17 percent and 16 percent, respectively. Of those who were diabetic (n=63), the majority were aware of their diabetes status (n=43) and were on treatment (n=30). Age, marital status, level of education, period of driving, obesity, sweet drinks consumption, physical activities and hypertension were significantly associated with diabetes. Only age >35 (AOR= 3.6, CI 1.2-11.1), ever married (AOR= 3.3, CI 1.5-7.0) and hypertension (AOR= 3.4, CI 1.7-6.8) were the independent predictors of diabetes after adjusting for confounders. The prevalence of metabolic syndrome was 22 percent. Age, marital status, level of education, physical inactivity and period of driving were significantly associated with metabolic syndrome. After adjusting for confounders, only age above 35 years (Adjusted Odd Ratio [AOR] =3.8, CI=1.4- 9.5), ever married (AOR=3.0, CI=1.6-5.3) and period of driving above five years (AOR= 2.4, CI=1.2-4.7) were the significant and independent predictors of metabolic syndrome. About 30 percent of the participants were daily smokers, 37 percent consume alcohol regularly and only 18 percent were physically active, while 82 percent were physically inactive. In conclusion, there was a high prevalence of obesity, hypertension and diabetes among the commercial drivers in this setting, which is associated with unhealthy lifestyles (smoking, excessive alcohol consumption, physical inactivity). Interventions aimed at promoting cardiovascular health of commercial drivers such as health education and workplace screening, should prioritize weight reduction, healthy eating and physical activity in this population.
- Full Text:
- Date Issued: 2018
- Authors: Adedokun, Aanuoluwa Odunayo
- Date: 2018
- Subjects: Diabetes -- South Africa -- Eastern Cape Obesity -- South Africa -- Eastern Cape Commercial vehicles
- Language: English
- Type: Thesis , Masters , Nursing Science
- Identifier: http://hdl.handle.net/10353/10431 , vital:35463
- Description: Cardio-metabolic risk factors are the aggregates of conditions that increase the susceptibility of developing cardio-metabolic diseases such as cardiovascular diseases (CVD) and diabetes. This includes insulin resistance, obesity, hyperglyceamia, dyslipoproteinemia, hyperinsulinemia and hypertension. This condition are worsen by smoking and physical inactivity as they mostlyare not easily detected. This was a cross-sectional survey of 403 commercial taxi drivers at ten different taxi ranks in BCMM. The study utilized a convenient sampling technique for the participants of the study. The WHO STEPwise approach was used for data collection. The WHO STEPwise questionnaire was used for a face-to-face interview. Socio-demographic (sex, age, marital status, driving experience, income) and behavioural characteristics (smoking, alcohol consumption, consumption of sweet drinks, physical activity, and dietary intake) of participants were obtained. Also, objective reports concerning anthropometric measurements (weight, height, waist and hip circumference), blood pressure and biochemical measurement that is the blood glucose measurement. All anthropometric measurements were taken following a standard procedure using a Lufkin nonextensible flexible anthropometric tape (W606PM), Rosscraft, Canada and a SECA weighing scale and stadiometer (Hamburg, Germany). Blood pressure was checked also in accordance with standard protocols using a Medic+ Digital Blood Pressure Monitor Model 1219 (Hamburg, Germany). A validated ACCU-CHEK glucose monitoring apparatus (Mannheim, Germany) wasused for blood glucose measurement. Overweight and obesity was defined as a body mass index (BMI) of 25.0 kg/m2 –29.9 kg/m2 and≥ 30 kg/m2, respectively. Pre-hypertension was defined according JNC-8 criteria a systolic blood pressure of 120- 139 mmHg and diastolic of BP 80-89 mmHg while hypertension was defined as an average of two systolic BP ≥140mmHg and/or diastolic BP of ≥90mmHg or a history of hypertension or anti hypertensive medication use. Diabetes status was determined using the fasting blood glucose (FBG) test and defined as a FBG ≥ 7.0mmol/L or self-reports of history or current diabetes medication use (treatment), while pre-diabetes was defined as a FBG of 5.6-6.9mmol/L. Awareness of diabetes was defined as self-reported history of diabetes among the people with diabetes. Metabolic syndrome status was determined according to the National agreement among makers of abdominal obesity. Statistical Package for Social Sciences (SPSS) was used for data analysis. The mean age of the participants was 43.3 (SD12.5) years. The prevalence of overweight and obesity was 34.0 percent and 38.0 percent, respectively. Age, marital status, period of driving, not-smoking, hypertension and diabetes were significantly associated with obesity. In logistic regression analysis, after adjusting for confounding factors, only age (OR 1.6, CI 1.0-2.7), hypertension (OR 3.6, CI 2.3-5.7) and non-smoking (OR 2.0, CI 1.3-3.1) were the independent and significant determinants of obesity. The prevalence rates of abdominal obesity by waist circumference (WC), waist-hip-ratio (WHR), waist-to-height ratio (WHtR) and neck circumference (NC) were 61.5 percent, 67.5 percent, 80.1 percent and 65.3 percent, respectively. A strong correlations exists between WHR and WC (>0.64); and WHtR and WC (>0.62). There exists a good agreement between WC and WHR. Also, a moderate agreement exists between WC and WHtR, WC and NC, WHR and WHtR, and WHtR and NC. The prevalence of above normal body composition for participants with hypertension was 71.0 percent, 65.9 percent, 63.2 percent and 66.5 percent by WC, WHR, WHtR and NC, respectively. For diabetes, the prevalence was 20.6 percent, 19.4 percent, 17.0 percent and 18.6 percent by WC, WHR, WHtR and NC, respectively. Waist circumference was a stronger predictor of hypertension and diabetics, with odds ratio of 3.7 (95 percent CI: 2.3-6.1 and 3.1 (95 percent CI: 1.6-6.0), compared to NC, with odds ratio of 1.7 (95 percent CI: 1.1-2.8). The prevalence of pre-hypertension was 33.7 percent and hypertension was 57.0 percent. Age, marital status, level of education, period of driving, obesity, alcohol, sweet drinks consumption and diabetes were significantly associated with hypertension. After adjusting for confounders, age >35 years (P=0.004), obesity and alcohol use (P<0.001), period of driving >5years (p=0.028) and diabetes (P=0.003) were significant predictors of hypertension. Prevalence of pre-diabetes and diabetes was 17 percent and 16 percent, respectively. Of those who were diabetic (n=63), the majority were aware of their diabetes status (n=43) and were on treatment (n=30). Age, marital status, level of education, period of driving, obesity, sweet drinks consumption, physical activities and hypertension were significantly associated with diabetes. Only age >35 (AOR= 3.6, CI 1.2-11.1), ever married (AOR= 3.3, CI 1.5-7.0) and hypertension (AOR= 3.4, CI 1.7-6.8) were the independent predictors of diabetes after adjusting for confounders. The prevalence of metabolic syndrome was 22 percent. Age, marital status, level of education, physical inactivity and period of driving were significantly associated with metabolic syndrome. After adjusting for confounders, only age above 35 years (Adjusted Odd Ratio [AOR] =3.8, CI=1.4- 9.5), ever married (AOR=3.0, CI=1.6-5.3) and period of driving above five years (AOR= 2.4, CI=1.2-4.7) were the significant and independent predictors of metabolic syndrome. About 30 percent of the participants were daily smokers, 37 percent consume alcohol regularly and only 18 percent were physically active, while 82 percent were physically inactive. In conclusion, there was a high prevalence of obesity, hypertension and diabetes among the commercial drivers in this setting, which is associated with unhealthy lifestyles (smoking, excessive alcohol consumption, physical inactivity). Interventions aimed at promoting cardiovascular health of commercial drivers such as health education and workplace screening, should prioritize weight reduction, healthy eating and physical activity in this population.
- Full Text:
- Date Issued: 2018
Assessing teaching strategies of grade four English second language teachers in Amathole District, Eastern Cape Province, South Africa
- Authors: Adegoriolu, Emmanuel Tobi
- Date: 2018
- Subjects: Education--South Africa English language--Study and teaching--Foreign speakers Second language acquisition
- Language: English
- Type: Thesis , Masters , Applied Linguistics
- Identifier: http://hdl.handle.net/10353/15474 , vital:40411
- Description: The intention of this study was to assess the teaching strategies of Grade 4 English second language teachers in the Amathole district of the Eastern Cape. The objectives of the study were firstly, to identify the teaching strategies currently employed by Grade 4 teachers in the teaching of English as a second language in selected schools in Amathole district of the Eastern Cape in South Africa. Secondly, the study sought to establish how the existing teaching strategies impacted on the performance of the learners of English as a second language in the selected schools in Amathloe district of the Eastern Cape, and to establish the teachers‟ perceptions on the most effective teaching strategies in the selected schools. The study used translanguaging and communicative language teaching as the theoretical lens and guide in data collection and analysis. The study employed the use of mixed methods of both quantitative and qualitative research design. Data was collected through the use of eight comprehensive classroom observations and eight teacher questionnaires in four different primary schools. The conclusion of the study as established in the outcome of data showed that there was no recommended teaching strategy by the Department of Education for English second language teachers teaching Grade 4. The study observed that the language teachers made use of various teaching strategies in the instruction of Grade 4 learners. In addition, it was concluded that the placement of the English language subject on the timetable affected the performance of young learners during the summer period. Moreover, the absence of teacher-learner cordial relationship compromised learning outcomes and performance of Grade 4 learners in the skills of English second language. The study recommends that teachers of English as a second language be trained on communicative language teaching in order to achieve positive outcomes in the classroom.
- Full Text:
- Date Issued: 2018
- Authors: Adegoriolu, Emmanuel Tobi
- Date: 2018
- Subjects: Education--South Africa English language--Study and teaching--Foreign speakers Second language acquisition
- Language: English
- Type: Thesis , Masters , Applied Linguistics
- Identifier: http://hdl.handle.net/10353/15474 , vital:40411
- Description: The intention of this study was to assess the teaching strategies of Grade 4 English second language teachers in the Amathole district of the Eastern Cape. The objectives of the study were firstly, to identify the teaching strategies currently employed by Grade 4 teachers in the teaching of English as a second language in selected schools in Amathole district of the Eastern Cape in South Africa. Secondly, the study sought to establish how the existing teaching strategies impacted on the performance of the learners of English as a second language in the selected schools in Amathloe district of the Eastern Cape, and to establish the teachers‟ perceptions on the most effective teaching strategies in the selected schools. The study used translanguaging and communicative language teaching as the theoretical lens and guide in data collection and analysis. The study employed the use of mixed methods of both quantitative and qualitative research design. Data was collected through the use of eight comprehensive classroom observations and eight teacher questionnaires in four different primary schools. The conclusion of the study as established in the outcome of data showed that there was no recommended teaching strategy by the Department of Education for English second language teachers teaching Grade 4. The study observed that the language teachers made use of various teaching strategies in the instruction of Grade 4 learners. In addition, it was concluded that the placement of the English language subject on the timetable affected the performance of young learners during the summer period. Moreover, the absence of teacher-learner cordial relationship compromised learning outcomes and performance of Grade 4 learners in the skills of English second language. The study recommends that teachers of English as a second language be trained on communicative language teaching in order to achieve positive outcomes in the classroom.
- Full Text:
- Date Issued: 2018
A qualitative assessment of the achievement of gender equality under education for all (EFA) goals : study of Lagos State, Nigeria
- Authors: Adeleke, Adawi Adeola
- Date: 2018
- Subjects: Sex discrimination in education -- Nigeria Sexism in education -- Nigeria Educational equalization -- Nigeria
- Language: English
- Type: Thesis , Doctoral , Education
- Identifier: http://hdl.handle.net/10353/9500 , vital:34357
- Description: The importance of education cannot be overemphasized. Education has been identified as the most critical instrument for empowering individuals with the necessary knowledge and skills, which can give them access to productive employment. Also, the future of a country is based on the quality of education acquired by its citizens which helps in transforming it from pre-literate to contemporary nationhood. Many international treaties and initiatives in the world have made special provisions for education as a duty of every government which must be given to every citizen as of right, irrespective of gender or social class. Amongst these international initiatives is the Education for All (EFA) Goals, a time-bound global movement towards the provision of education for every one led by the United Nations Educational, Scientific and Cultural Organization (UNESCO). There are six goals in all. This study investigated one of the EFA goals, the goal 5. The aim of the study was a qualitative assessment of the achievement of gender equality goal under the Education for All (EFA) goals, a study of Lagos State, Nigeria. The theoretical framework of the study is informed by the Social Justice Theory and the Social Justice Praxis Theory. Scholarly works and literatures on girl-child education, gender and education, and gender balancing in education were reviewed. The study followed qualitative research approach which utilizes an interpretive research paradigm that provides a collective voice from the participants. The research area was purposively selected as Lagos State being a former capital of Nigeria and still the commercial nerve centre of the nation. Although it is the smallest state in Nigeria in term of size, it remains the most populous in the country with a projected population of 20,546,999 (13.5percent Nigeria’s total population). A sample of 16 participants were purposively selected because they were the officers directly in charge of the implementation government policies on education in the State. The data was collected through in-depth recorded interview sessions and secondary data were sourced through official documents downloaded online and from Lagos State Ministry of Education. Based on the thematic analysis of the data collected, the study found out that the State government had established policies and measures to provide qualitative education for all the citizens on a non-discriminatory basis. Thus, the goal of gender parity in terms of enrollment of boys and girls has been relatively achieved in the state. However, there were still some challenges of equality in the learning process and outcome. The study therefore recommends that schools should be made attractive and conducive as possible to assist the educational attainment of any student. This could be achieved by promoting positive values and attitudes towards education, all the stakeholders in the education sector must be involved in the business of running the school. There should also be a form of resourcing and expansion of the school facilities through the Public - Private Partnership in education.
- Full Text:
- Date Issued: 2018
- Authors: Adeleke, Adawi Adeola
- Date: 2018
- Subjects: Sex discrimination in education -- Nigeria Sexism in education -- Nigeria Educational equalization -- Nigeria
- Language: English
- Type: Thesis , Doctoral , Education
- Identifier: http://hdl.handle.net/10353/9500 , vital:34357
- Description: The importance of education cannot be overemphasized. Education has been identified as the most critical instrument for empowering individuals with the necessary knowledge and skills, which can give them access to productive employment. Also, the future of a country is based on the quality of education acquired by its citizens which helps in transforming it from pre-literate to contemporary nationhood. Many international treaties and initiatives in the world have made special provisions for education as a duty of every government which must be given to every citizen as of right, irrespective of gender or social class. Amongst these international initiatives is the Education for All (EFA) Goals, a time-bound global movement towards the provision of education for every one led by the United Nations Educational, Scientific and Cultural Organization (UNESCO). There are six goals in all. This study investigated one of the EFA goals, the goal 5. The aim of the study was a qualitative assessment of the achievement of gender equality goal under the Education for All (EFA) goals, a study of Lagos State, Nigeria. The theoretical framework of the study is informed by the Social Justice Theory and the Social Justice Praxis Theory. Scholarly works and literatures on girl-child education, gender and education, and gender balancing in education were reviewed. The study followed qualitative research approach which utilizes an interpretive research paradigm that provides a collective voice from the participants. The research area was purposively selected as Lagos State being a former capital of Nigeria and still the commercial nerve centre of the nation. Although it is the smallest state in Nigeria in term of size, it remains the most populous in the country with a projected population of 20,546,999 (13.5percent Nigeria’s total population). A sample of 16 participants were purposively selected because they were the officers directly in charge of the implementation government policies on education in the State. The data was collected through in-depth recorded interview sessions and secondary data were sourced through official documents downloaded online and from Lagos State Ministry of Education. Based on the thematic analysis of the data collected, the study found out that the State government had established policies and measures to provide qualitative education for all the citizens on a non-discriminatory basis. Thus, the goal of gender parity in terms of enrollment of boys and girls has been relatively achieved in the state. However, there were still some challenges of equality in the learning process and outcome. The study therefore recommends that schools should be made attractive and conducive as possible to assist the educational attainment of any student. This could be achieved by promoting positive values and attitudes towards education, all the stakeholders in the education sector must be involved in the business of running the school. There should also be a form of resourcing and expansion of the school facilities through the Public - Private Partnership in education.
- Full Text:
- Date Issued: 2018
Analysis of the security and reliability of packet transmission in Wireless Mesh Networks (WMNs) : a case study of Malicious Packet drop attack
- Adeniji, Victor Oluwatobiloba
- Authors: Adeniji, Victor Oluwatobiloba
- Date: 2018
- Subjects: Wireless communication systems Mobile communication systems
- Language: English
- Type: Thesis , Masters , Computer Science
- Identifier: http://hdl.handle.net/10353/17450 , vital:40969
- Description: Wireless Mesh Networks (WMNs) are known for possessing good attributes such as low up-front cost, easy network maintenance, and reliable service coverage. This has largely made them to be adopted in various areas such as; school campus networks, community networking, pervasive healthcare, office and home automation, emergency rescue operations and ubiquitous wireless networks. The routing nodes are equipped with self-organized and self-configuring capabilities. The routing mechanisms of WMNs depend on the collaboration of all participating nodes for reliable network performance. However, it has been noted that most routing algorithms proposed for WMNs in the last few years are designed with the assumption that all the participating nodes will collaboratively be involved in relaying the data packets originated from a source to a multi-hop destination. Such design approach exposes WMNs to vulnerability such as malicious packet drop attack. Therefore, it is imperative to design and implement secure and reliable packet routing mechanisms to mitigate this type of attack. While there are works that have attempted to implement secure routing approach, the findings in this research unearthed that further research works are required to improve the existing secure routing in order to provide more secure and reliable packet transmission in WMNs, in the event of denial of service (DoS) attacks such black hole malicious pack drop attack. This study further presents an analysis of the impact of the black hole malicious packet drop attack with other influential factors in WMNs. In the study, NS-3 simulator was used with AODV as the routing protocol. The results show that the packet delivery ratio and throughput of WMN under attack decreases sharply as compared to WMN free from attack.
- Full Text:
- Date Issued: 2018
- Authors: Adeniji, Victor Oluwatobiloba
- Date: 2018
- Subjects: Wireless communication systems Mobile communication systems
- Language: English
- Type: Thesis , Masters , Computer Science
- Identifier: http://hdl.handle.net/10353/17450 , vital:40969
- Description: Wireless Mesh Networks (WMNs) are known for possessing good attributes such as low up-front cost, easy network maintenance, and reliable service coverage. This has largely made them to be adopted in various areas such as; school campus networks, community networking, pervasive healthcare, office and home automation, emergency rescue operations and ubiquitous wireless networks. The routing nodes are equipped with self-organized and self-configuring capabilities. The routing mechanisms of WMNs depend on the collaboration of all participating nodes for reliable network performance. However, it has been noted that most routing algorithms proposed for WMNs in the last few years are designed with the assumption that all the participating nodes will collaboratively be involved in relaying the data packets originated from a source to a multi-hop destination. Such design approach exposes WMNs to vulnerability such as malicious packet drop attack. Therefore, it is imperative to design and implement secure and reliable packet routing mechanisms to mitigate this type of attack. While there are works that have attempted to implement secure routing approach, the findings in this research unearthed that further research works are required to improve the existing secure routing in order to provide more secure and reliable packet transmission in WMNs, in the event of denial of service (DoS) attacks such black hole malicious pack drop attack. This study further presents an analysis of the impact of the black hole malicious packet drop attack with other influential factors in WMNs. In the study, NS-3 simulator was used with AODV as the routing protocol. The results show that the packet delivery ratio and throughput of WMN under attack decreases sharply as compared to WMN free from attack.
- Full Text:
- Date Issued: 2018
Potential latitudinal variation in orodigestive tract cancers in Africa
- Adeola, H A, Adefuye, A O, Jimoh, S A
- Authors: Adeola, H A , Adefuye, A O , Jimoh, S A
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/5280 , vital:44428 , http://www.samj.org.za/index.php/samj/article/view/12259
- Description: Background. Previous studies have alluded to a causal relationship between pathological entities and geographical variations, but there is a paucity of studies from Africa discussing the effect of latitudinal variation on orodigestive cancers in this region. It seems plausible that the burden of orodigestive cancer would differ as a result of variations in diet, cultural habits, climate and environmental conditions down the length of Africa. Objectives. To analyse regional variations in prevalence, incidence and mortality data in the global cancer statistics database (GLOBOCAN 2012) curated by the World Health Organization and the International Agency for Research on Cancer. Basic descriptive statistical tools were used to depict regional variations in cancer morbidity and mortality. Methods. Data on 13 African countries between longitude 20⁰ and 30⁰ east and latitude 35⁰ north and 35⁰ south were examined for variation in age-standardised orodigestive cancer prevalence, incidence and mortality. Possible regional causes for orodigestive tract cancer development were investigated. Data on lip and oral cavity, oesophageal, gastric, colorectal, liver, gallbladder and pancreatic cancers in the 13 countries were compared. Results. Our empirical findings from this preliminary study support the notion that the incidence and prevalence of orodigestive cancers vary within Africa. This effect may be due to environmental, economic, political and possibly genetic factors. Conclusions. Considering the heterogeneity of the above factors across Africa, disbursement of funding for cancer research and therapy in Africa should be focused in terms of regional variations to make best use of the fiscal allocation by African governments, non-governmental organisations and international agencies.
- Full Text:
- Date Issued: 2018
- Authors: Adeola, H A , Adefuye, A O , Jimoh, S A
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/5280 , vital:44428 , http://www.samj.org.za/index.php/samj/article/view/12259
- Description: Background. Previous studies have alluded to a causal relationship between pathological entities and geographical variations, but there is a paucity of studies from Africa discussing the effect of latitudinal variation on orodigestive cancers in this region. It seems plausible that the burden of orodigestive cancer would differ as a result of variations in diet, cultural habits, climate and environmental conditions down the length of Africa. Objectives. To analyse regional variations in prevalence, incidence and mortality data in the global cancer statistics database (GLOBOCAN 2012) curated by the World Health Organization and the International Agency for Research on Cancer. Basic descriptive statistical tools were used to depict regional variations in cancer morbidity and mortality. Methods. Data on 13 African countries between longitude 20⁰ and 30⁰ east and latitude 35⁰ north and 35⁰ south were examined for variation in age-standardised orodigestive cancer prevalence, incidence and mortality. Possible regional causes for orodigestive tract cancer development were investigated. Data on lip and oral cavity, oesophageal, gastric, colorectal, liver, gallbladder and pancreatic cancers in the 13 countries were compared. Results. Our empirical findings from this preliminary study support the notion that the incidence and prevalence of orodigestive cancers vary within Africa. This effect may be due to environmental, economic, political and possibly genetic factors. Conclusions. Considering the heterogeneity of the above factors across Africa, disbursement of funding for cancer research and therapy in Africa should be focused in terms of regional variations to make best use of the fiscal allocation by African governments, non-governmental organisations and international agencies.
- Full Text:
- Date Issued: 2018
Factors affecting adherence to antiretroviral therapy among pregnant women in theEastern Cape, South Africa
- Adeola, H A, Adeniyi, Oladele Vincent, Ajayi, Anthony Idowu, Goon, Daniel Ter, Owolabi, Eyitayo Omolara, Eboh, Alfred, Lambert, John
- Authors: Adeola, H A , Adeniyi, Oladele Vincent , Ajayi, Anthony Idowu , Goon, Daniel Ter , Owolabi, Eyitayo Omolara , Eboh, Alfred , Lambert, John
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/5272 , vital:44427 , https://neuro.unboundmedicine.com/medline/citation/29653510/Factors_affecting_adherence_to_antiretroviral_therapy_among_pregnant_women_in_the_Eastern_Cape_South_Africa_
- Description: Background:Context-specific factors influence adherence to antiretroviral therapy (ART) among pregnant womenliving with HIV. Gaps exist in the understanding of the reasons for the variable outcomes of the prevention ofmother-to-child transmission (PMTCT) programme at the health facility level in South Africa. This study examinedadherence levels and reasons for non-adherence during pregnancy in a cohort of parturient women enrolled in thePMTCT programme in the Eastern Cape, South Africa.Methods:This was a mixed-methods study involving 1709 parturient women in the Eastern Cape, South Africa. Weconducted a multi-centre retrospective analysis of the mother-infant pair in the PMTCT electronic database in 2016.Semi-structured interviews of purposively selected parturient women with self-reported poor adherence (n= 177)were conducted to gain understanding of the main barriers to adherence. Binary logistic regression was used todetermine the independent predictors of ART non-adherence.Results:A high proportion (69.0%) of women reported perfect adherence. In the logistic regression analysis, afteradjusting for confounding factors, marital status, cigarette smoking, alcohol use and non-disclosure to a family memberwere the independent predictors of non-adherence. Analysis of the qualitative data revealed that drug-related side-effects, being away from home, forgetfulness, non-disclosure, stigma and work-related demand were among the mainreasons for non-adherence to ART.Conclusions:Non-adherence to the antiretroviral therapy among pregnant women in this setting is associated withlifestyle behaviours, HIV-related stigma and ART side-effects. In order to eliminate mother-to-child transmission of HIV,clinicians need to screen for these factors at every antenatal clinic visit.Keywords:Adherence, Non-adherence, HIV, Antiretroviral therapy, Elimination of mother-to-child transmission,Prevention of mother-to-child transmission, Stigma, South Africa
- Full Text:
- Date Issued: 2018
- Authors: Adeola, H A , Adeniyi, Oladele Vincent , Ajayi, Anthony Idowu , Goon, Daniel Ter , Owolabi, Eyitayo Omolara , Eboh, Alfred , Lambert, John
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/5272 , vital:44427 , https://neuro.unboundmedicine.com/medline/citation/29653510/Factors_affecting_adherence_to_antiretroviral_therapy_among_pregnant_women_in_the_Eastern_Cape_South_Africa_
- Description: Background:Context-specific factors influence adherence to antiretroviral therapy (ART) among pregnant womenliving with HIV. Gaps exist in the understanding of the reasons for the variable outcomes of the prevention ofmother-to-child transmission (PMTCT) programme at the health facility level in South Africa. This study examinedadherence levels and reasons for non-adherence during pregnancy in a cohort of parturient women enrolled in thePMTCT programme in the Eastern Cape, South Africa.Methods:This was a mixed-methods study involving 1709 parturient women in the Eastern Cape, South Africa. Weconducted a multi-centre retrospective analysis of the mother-infant pair in the PMTCT electronic database in 2016.Semi-structured interviews of purposively selected parturient women with self-reported poor adherence (n= 177)were conducted to gain understanding of the main barriers to adherence. Binary logistic regression was used todetermine the independent predictors of ART non-adherence.Results:A high proportion (69.0%) of women reported perfect adherence. In the logistic regression analysis, afteradjusting for confounding factors, marital status, cigarette smoking, alcohol use and non-disclosure to a family memberwere the independent predictors of non-adherence. Analysis of the qualitative data revealed that drug-related side-effects, being away from home, forgetfulness, non-disclosure, stigma and work-related demand were among the mainreasons for non-adherence to ART.Conclusions:Non-adherence to the antiretroviral therapy among pregnant women in this setting is associated withlifestyle behaviours, HIV-related stigma and ART side-effects. In order to eliminate mother-to-child transmission of HIV,clinicians need to screen for these factors at every antenatal clinic visit.Keywords:Adherence, Non-adherence, HIV, Antiretroviral therapy, Elimination of mother-to-child transmission,Prevention of mother-to-child transmission, Stigma, South Africa
- Full Text:
- Date Issued: 2018
Bioprospecting for amylases, cellulases and xylanases from ericoid associated fungi, their production and characterisation for the bio-economy
- Authors: Adeoyo, Olusegun Richard
- Date: 2018
- Subjects: Mycorrhizal fungi , Hydrolases , Ericaceae South Africa , Ericaceae Molecular aspects
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/64327 , vital:28533
- Description: South Africa is one of the most productive areas for ericaceous plants with about 850 identified species in the Cape Floral Region. The Albany Centre of Endemism where all fungi used in this study were isolated from, falls within this region. Ericaceous plants interact with some fungi via an association called the ericoid mycorrhizal (ERM) association. All fungi used in this study were isolated from roots of six ericaceous plants; Erica cerinthoides, Erica demissa, Erica chamissonis, Erica glumiflora, Erica caffra and Erica nemorosa. Fungal enzymes are known to play a significant role in the food, brewing, detergent, pharmaceutical and biofuel industries. The enzyme industry is among the major sectors of the world, and additional novel sources are being explored from time to time. This study focussed on amylases (amyloglucosidase, AMG), cellulases (endoglucanase) and xylanases (endo-1,4-P-xylanase) production from ERM fungal isolates. Out of the fifty-one (51), fungal isolates screened, ChemRU330 (Leohumicola sp.), EdRU083 and EdRU002 were among the fungi that had the highest activities of all the enzymes. They were tested for the ability to produce amylases and cellulases under different pH and nutritional conditions that included: carbon sources, nitrogen sources and metal ions, at an optimum temperature of 28°C in a modified Melin-Norkrans (MMN) liquid medium. Cellulase specific activity of 3.99, 2.18 and 4.31 (U/mg protein) for isolates EdRU083, EdRU002 and ChemRU330, respectively, was produced at an optimal pH of 5.0. For amylase, ChemRU330 had the highest specific activity of 1.11 U/mg protein while EdRU083 and EdRU02 had a specific activity of 0.80 and 0.92 U/mg protein, respectively, at the same pH with corresponding biomass yield of 113, 125 and 97 mg/50 ml, respectively. Increased enzyme activities and improved mycelial biomass production were obtained in the presence of supplements such as potassium, sodium, glucose, maltose, cellobiose, tryptone and peptone, while NaFe-EDTA and cobalt inhibited enzyme activity. ChemRU330 was selected to determine the consistency and amount of amylase, cellulase and xylanase formed after several in vitro subculturing events. AMG and endo-1,4-P-xylanase were found to have the most consistent production throughout the study period. The AMG was stable at 45oC (pH 5.0), retaining approximately 65% activity over a period of 24 h. The molecular mass of AMG and endo-1,4-P-xylanase were estimated to be 101 kDa and 72 kDa, respectively. The Km and kcat were 0.38 mg/ml and 70 s-1, respectively, using soluble starch (AMG). For endo-1,4-P-xylanase, the Km and Vmax were 0.93 mg/ml and 8.54 U/ml, respectively, using beechwood xylan (endo-1,4-P-xylanase) as substrate. Additionally, crude extracts of five root endophytes with unique morphological characteristics were screened for antibacterial properties and was followed by determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). L. incrustata (ChemRU330) and Chaetomium sp. extracts exhibited varying degrees of inhibition against two Gram-positive and Gram-negative bacteria. The crude extract of L. incrustata was the most effective which was found to inhibit Staphylococcus aureus (MIC: 1 mg/ml), Bacillus subtilis (MIC: 2 mg/ml) and Proteus vulgaris (MIC: 16 mg/ml). The L. incrustata displayed potential for antibacterial production and could be considered as an additional source of new antimicrobial agents in drug and food preservation. Also, the three isolates used for enzyme production were identified to genus and species levels, i.e., Leohumicola incrustata (ChemRU330), Leohumicola sp. (EdRU083) and Oidiodendron sp. (EdRU002) using both ITS and Cox1 DNA regions. The molecular analysis results indicated that these ERM mycorrhizal fungi were similar to those successfully described by some researchers in South Africa and Australia. Therefore, this study opens new opportunities for exploring ERM fungal biomolecules for the bio-economy. The promising physicochemical properties, starch and xylan hydrolysis end- products, and being non-pathogenic make AMG and endo-1,4-P-xylanase potential candidates for future applications as additives in the food industry for the production of glucose, glucose syrups, high-fructose corn syrups, and as well as the production of bioethanol. Finally, the findings of this study revealed that it is possible to produce hydrolytic enzymes from ERM fungi in vitro using chemically defined media. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2018
- Full Text:
- Date Issued: 2018
- Authors: Adeoyo, Olusegun Richard
- Date: 2018
- Subjects: Mycorrhizal fungi , Hydrolases , Ericaceae South Africa , Ericaceae Molecular aspects
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/64327 , vital:28533
- Description: South Africa is one of the most productive areas for ericaceous plants with about 850 identified species in the Cape Floral Region. The Albany Centre of Endemism where all fungi used in this study were isolated from, falls within this region. Ericaceous plants interact with some fungi via an association called the ericoid mycorrhizal (ERM) association. All fungi used in this study were isolated from roots of six ericaceous plants; Erica cerinthoides, Erica demissa, Erica chamissonis, Erica glumiflora, Erica caffra and Erica nemorosa. Fungal enzymes are known to play a significant role in the food, brewing, detergent, pharmaceutical and biofuel industries. The enzyme industry is among the major sectors of the world, and additional novel sources are being explored from time to time. This study focussed on amylases (amyloglucosidase, AMG), cellulases (endoglucanase) and xylanases (endo-1,4-P-xylanase) production from ERM fungal isolates. Out of the fifty-one (51), fungal isolates screened, ChemRU330 (Leohumicola sp.), EdRU083 and EdRU002 were among the fungi that had the highest activities of all the enzymes. They were tested for the ability to produce amylases and cellulases under different pH and nutritional conditions that included: carbon sources, nitrogen sources and metal ions, at an optimum temperature of 28°C in a modified Melin-Norkrans (MMN) liquid medium. Cellulase specific activity of 3.99, 2.18 and 4.31 (U/mg protein) for isolates EdRU083, EdRU002 and ChemRU330, respectively, was produced at an optimal pH of 5.0. For amylase, ChemRU330 had the highest specific activity of 1.11 U/mg protein while EdRU083 and EdRU02 had a specific activity of 0.80 and 0.92 U/mg protein, respectively, at the same pH with corresponding biomass yield of 113, 125 and 97 mg/50 ml, respectively. Increased enzyme activities and improved mycelial biomass production were obtained in the presence of supplements such as potassium, sodium, glucose, maltose, cellobiose, tryptone and peptone, while NaFe-EDTA and cobalt inhibited enzyme activity. ChemRU330 was selected to determine the consistency and amount of amylase, cellulase and xylanase formed after several in vitro subculturing events. AMG and endo-1,4-P-xylanase were found to have the most consistent production throughout the study period. The AMG was stable at 45oC (pH 5.0), retaining approximately 65% activity over a period of 24 h. The molecular mass of AMG and endo-1,4-P-xylanase were estimated to be 101 kDa and 72 kDa, respectively. The Km and kcat were 0.38 mg/ml and 70 s-1, respectively, using soluble starch (AMG). For endo-1,4-P-xylanase, the Km and Vmax were 0.93 mg/ml and 8.54 U/ml, respectively, using beechwood xylan (endo-1,4-P-xylanase) as substrate. Additionally, crude extracts of five root endophytes with unique morphological characteristics were screened for antibacterial properties and was followed by determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). L. incrustata (ChemRU330) and Chaetomium sp. extracts exhibited varying degrees of inhibition against two Gram-positive and Gram-negative bacteria. The crude extract of L. incrustata was the most effective which was found to inhibit Staphylococcus aureus (MIC: 1 mg/ml), Bacillus subtilis (MIC: 2 mg/ml) and Proteus vulgaris (MIC: 16 mg/ml). The L. incrustata displayed potential for antibacterial production and could be considered as an additional source of new antimicrobial agents in drug and food preservation. Also, the three isolates used for enzyme production were identified to genus and species levels, i.e., Leohumicola incrustata (ChemRU330), Leohumicola sp. (EdRU083) and Oidiodendron sp. (EdRU002) using both ITS and Cox1 DNA regions. The molecular analysis results indicated that these ERM mycorrhizal fungi were similar to those successfully described by some researchers in South Africa and Australia. Therefore, this study opens new opportunities for exploring ERM fungal biomolecules for the bio-economy. The promising physicochemical properties, starch and xylan hydrolysis end- products, and being non-pathogenic make AMG and endo-1,4-P-xylanase potential candidates for future applications as additives in the food industry for the production of glucose, glucose syrups, high-fructose corn syrups, and as well as the production of bioethanol. Finally, the findings of this study revealed that it is possible to produce hydrolytic enzymes from ERM fungi in vitro using chemically defined media. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2018
- Full Text:
- Date Issued: 2018