Application of bidentate N,N'-donor extractants in the hydrometallurgical separation of base metals from an acidic sulfate medium
- Authors: Okewole, Adeleye Ishola
- Date: 2013
- Subjects: Hydrometallurgy Sulfates Nanofibers Electrospinning Sorbents Extraction (Chemistry) Solvent extraction Stereochemistry
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4281 , http://hdl.handle.net/10962/d1002610
- Description: Bidentate imidazole-based extractants, 1-octylimidazole-2-aldoxime (OIMOX) and 1-octyl-2-(2′-pyridyl)imidazole (OPIM), along with dinonylnaphthalene sulfonic acid (DNNSA) as a synergist, were investigated as potential selective extractants for Cu²⁺and Ni²⁺ respectively from base metals in a solvent extraction system. The study was extended to evaluate the sorption and separation of Ni²⁺ from other base metals in a solid-solution system using microspherical Merrifield resins and nanofibers functionalized with 2,2′-pyridylimidazole. Copper was effectively separated with OIMOX and DNNSA as extractants from nickel with ΔpH½ ≈1.05 and the extraction order of Cu²⁺ > Ni²⁺ > Zn²⁺ > Cd²⁺> Co²⁺ was achieved as a function of pH. At pH 1.65 the extracted copper, from a synthetic mixture of the base metals reached 90.13(±0.90)%, and through a two-step extraction process 98.22(±0.29)% copper was recovered with negligible nickel and cobalt impurities. Stripping of the copper from the loaded organic phase using TraceSelect sulphuric acid at pH 0.35 yielded 96.60(±0.44)% of the loaded quantity after the second stage of stripping. The separation of Ni²⁺ from the borderline and hard acids; Co²⁺, Cu²⁺, Zn²⁺, Fe2²⁺, Fe²⁺, Mn²⁺, Mg2²⁺ and Ca²⁺ at a pH range of 0.5-3.5 with OPIM and DNNSA was acvieved to the tune of a ΔpH½≈ 1.6 with respect to cobalt from a sulfate and sulfate/chloride media. A three-stage counter-current extraction of Ni²⁺, at the optimized pH of 1.89, from a synthetic mixture of Ni²⁺, Co²⁺ and Cu²⁺, yielded 99.01(±1.79)%. The total co-extracted Cu²⁺ was 48.72(±0.24)% of the original quantity in the mixture, and it was 19.85(±0.28%) for Co²⁺. The co-extracted Cu²⁺ was scrubbed off from the loaded organic phase at pH≈8.5 by using an ammonium buffer, while co-extracted Co²⁺ was selectively and quantitatively stripped with H₂SO₄ at pH 1.64. The total recovery of Ni²⁺ by stripping at pH 0.32 was 94.05(±1.70)%. In the solid-liquid system, Ni²⁺ was separated from Co²⁺, Cu²⁺, and Fe²⁺ with the microspherical resins funtionalised with 2,2′-pyridylimidazole by a separation factor (β) in the range 22-45. Electrospun nanofibers as sorbents yielded high sorption capacity in the range of 0.97 - 1.45 mmol.g⁻¹ for the same metals ions. Thus, 1-octylimidazole-2-aldoxime (OIMOX), and1-octyl-2-(2′-pyridyl)imidazole (OPIM) can be effectively utilized alongside DNNSA as a co-extractant in the separation of Cu²⁺ and Ni²⁺ respectively from base metals in acidic sulfate medium in a solvent extraction process, and the latter as a selective ligand in the solid-liquid separation of Ni²⁺ from Co²⁺, Cu²⁺, and Fe²⁺.
- Full Text:
- Date Issued: 2013
- Authors: Okewole, Adeleye Ishola
- Date: 2013
- Subjects: Hydrometallurgy Sulfates Nanofibers Electrospinning Sorbents Extraction (Chemistry) Solvent extraction Stereochemistry
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4281 , http://hdl.handle.net/10962/d1002610
- Description: Bidentate imidazole-based extractants, 1-octylimidazole-2-aldoxime (OIMOX) and 1-octyl-2-(2′-pyridyl)imidazole (OPIM), along with dinonylnaphthalene sulfonic acid (DNNSA) as a synergist, were investigated as potential selective extractants for Cu²⁺and Ni²⁺ respectively from base metals in a solvent extraction system. The study was extended to evaluate the sorption and separation of Ni²⁺ from other base metals in a solid-solution system using microspherical Merrifield resins and nanofibers functionalized with 2,2′-pyridylimidazole. Copper was effectively separated with OIMOX and DNNSA as extractants from nickel with ΔpH½ ≈1.05 and the extraction order of Cu²⁺ > Ni²⁺ > Zn²⁺ > Cd²⁺> Co²⁺ was achieved as a function of pH. At pH 1.65 the extracted copper, from a synthetic mixture of the base metals reached 90.13(±0.90)%, and through a two-step extraction process 98.22(±0.29)% copper was recovered with negligible nickel and cobalt impurities. Stripping of the copper from the loaded organic phase using TraceSelect sulphuric acid at pH 0.35 yielded 96.60(±0.44)% of the loaded quantity after the second stage of stripping. The separation of Ni²⁺ from the borderline and hard acids; Co²⁺, Cu²⁺, Zn²⁺, Fe2²⁺, Fe²⁺, Mn²⁺, Mg2²⁺ and Ca²⁺ at a pH range of 0.5-3.5 with OPIM and DNNSA was acvieved to the tune of a ΔpH½≈ 1.6 with respect to cobalt from a sulfate and sulfate/chloride media. A three-stage counter-current extraction of Ni²⁺, at the optimized pH of 1.89, from a synthetic mixture of Ni²⁺, Co²⁺ and Cu²⁺, yielded 99.01(±1.79)%. The total co-extracted Cu²⁺ was 48.72(±0.24)% of the original quantity in the mixture, and it was 19.85(±0.28%) for Co²⁺. The co-extracted Cu²⁺ was scrubbed off from the loaded organic phase at pH≈8.5 by using an ammonium buffer, while co-extracted Co²⁺ was selectively and quantitatively stripped with H₂SO₄ at pH 1.64. The total recovery of Ni²⁺ by stripping at pH 0.32 was 94.05(±1.70)%. In the solid-liquid system, Ni²⁺ was separated from Co²⁺, Cu²⁺, and Fe²⁺ with the microspherical resins funtionalised with 2,2′-pyridylimidazole by a separation factor (β) in the range 22-45. Electrospun nanofibers as sorbents yielded high sorption capacity in the range of 0.97 - 1.45 mmol.g⁻¹ for the same metals ions. Thus, 1-octylimidazole-2-aldoxime (OIMOX), and1-octyl-2-(2′-pyridyl)imidazole (OPIM) can be effectively utilized alongside DNNSA as a co-extractant in the separation of Cu²⁺ and Ni²⁺ respectively from base metals in acidic sulfate medium in a solvent extraction process, and the latter as a selective ligand in the solid-liquid separation of Ni²⁺ from Co²⁺, Cu²⁺, and Fe²⁺.
- Full Text:
- Date Issued: 2013
Aspects of isiXhosa poetry with special reference to poems produced about women
- Jadezweni, Mhlobo Wabantwana
- Authors: Jadezweni, Mhlobo Wabantwana
- Date: 2013
- Subjects: Laudatory poetry, Xhosa African poetry (English) -- History and criticism Women in literature Women and literature -- South Africa African poetry -- Women authors
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3616 , http://hdl.handle.net/10962/d1006364
- Description: This study investigates the use of modern and izibongo (praise poetry) techniques in representing women in selected isiXhosa poems. The main interest of the study is to determine whether the same techniques to depict men are used when writing about women. It is also the interest of the study to ascertain how gender issues are dealt with in the selected poems. Seminal studies on izibongo by eminent scholars in this field show a serious lack of critique and little recognition of women in African languages’ poetry in general and in isiXhosa in particular. Pioneering studies in Nguni poetry about women have thus recommended that serious studies on poetry about women be undertaken. The analyses of selected poems by established isiXhosa poets show that modern poetry conventions are significantly used together with izibongo techniques. These techniques are used without any gender differentiation, which is another point of interest of this study. There are however instances where images specific to women are used. Such use has however not been found to be demeaning of women in any way. Poems where modern poetry forms and conventions are used tend to deal with subjects who have international or an urban area background. Even though the modern poetry conventions are used with izibongo techniques the presence of the modern literary conventions is prominent. This is the case particularly with poems about women in politics. That some female poet seems to accept some cultural practices that are viewed to be undermining the status of women does not take away the voice of protest against this oppression by some of the selected poets. These two voices, one of acceptance and the other one of protest are used as a basis for a debate around a need for a literary theory that addresses the question of African culture with special reference to isiXhosa poetry about women. The success of the selected poets with both modern and izibongo techniques is a good sign for the development of isiXhosa poetry in general and isiXhosa poetry about women. It is strongly recommended that continued research of a serious nature concerning poetry about, and produced by women, be undertaken.
- Full Text:
- Date Issued: 2013
- Authors: Jadezweni, Mhlobo Wabantwana
- Date: 2013
- Subjects: Laudatory poetry, Xhosa African poetry (English) -- History and criticism Women in literature Women and literature -- South Africa African poetry -- Women authors
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3616 , http://hdl.handle.net/10962/d1006364
- Description: This study investigates the use of modern and izibongo (praise poetry) techniques in representing women in selected isiXhosa poems. The main interest of the study is to determine whether the same techniques to depict men are used when writing about women. It is also the interest of the study to ascertain how gender issues are dealt with in the selected poems. Seminal studies on izibongo by eminent scholars in this field show a serious lack of critique and little recognition of women in African languages’ poetry in general and in isiXhosa in particular. Pioneering studies in Nguni poetry about women have thus recommended that serious studies on poetry about women be undertaken. The analyses of selected poems by established isiXhosa poets show that modern poetry conventions are significantly used together with izibongo techniques. These techniques are used without any gender differentiation, which is another point of interest of this study. There are however instances where images specific to women are used. Such use has however not been found to be demeaning of women in any way. Poems where modern poetry forms and conventions are used tend to deal with subjects who have international or an urban area background. Even though the modern poetry conventions are used with izibongo techniques the presence of the modern literary conventions is prominent. This is the case particularly with poems about women in politics. That some female poet seems to accept some cultural practices that are viewed to be undermining the status of women does not take away the voice of protest against this oppression by some of the selected poets. These two voices, one of acceptance and the other one of protest are used as a basis for a debate around a need for a literary theory that addresses the question of African culture with special reference to isiXhosa poetry about women. The success of the selected poets with both modern and izibongo techniques is a good sign for the development of isiXhosa poetry in general and isiXhosa poetry about women. It is strongly recommended that continued research of a serious nature concerning poetry about, and produced by women, be undertaken.
- Full Text:
- Date Issued: 2013
Assessing household assets to understand vulnerability to HIV/Aids and climate change in the Eastern Cape, South Africa
- Authors: Stadler, Leigh Tessa
- Date: 2013
- Subjects: Climatic changes -- South Africa -- Eastern Cape Climatic changes -- Health aspects -- South Africa -- Eastern Cape Climatic changes -- Economic aspects -- South Africa -- Eastern Cape HIV infections -- Economic aspects -- South Africa -- Eastern Cape AIDS (Disease) -- Economic aspects -- South Africa -- Eastern Cape HIV infections -- Social aspects -- South Africa -- Eastern Cape AIDS (Disease) -- Social aspects -- South Africa -- Eastern Cape Poverty -- Social aspects -- South Africa -- Eastern Cape Income -- South Africa -- Eastern Cape Human capital -- South Africa -- Eastern Cape Sex role -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4726 , http://hdl.handle.net/10962/d1001655
- Description: Livelihood stressors in southern Africa, such as HIV/Aids and climate change, do not act in isolation but rather interact concurrently in complex socio-ecological systems with diverse, interrelated and compounded affects. Households experience differential vulnerability to such stressors based on contextual factors such as geographical location, income level and the gender and age of its members. Households’ differential experiences of vulnerability are further defined by the households’ use of their capital stocks: the human, social, natural, financial and physical capital available to the household to form livelihoods and resist the detrimental effects of a stressor. The capital stocks of 340 households were measured in two sites in the Eastern Cape, South Africa, using a household survey. These data were analysed to determine differences between the sites, households with heads of different gender and households of different income levels. Further data relating to the drivers and interactions of stressors over temporal and spatial scales, as well as the perceived value of various forms of capital by different social groups in the two sites, were collected via Participatory Learning and Action (PLA) methods including timelines, mental modelling and pair-wise ranking. Although the two sites have similar levels of income and fall within the same province, many significant differences emerged. The two sites showed different distributions of household head genders and different stressors and perceptions of vulnerability, perhaps owing to differences in their capital stocks, acting alongside the influence of culture and access on a shifting rural-urban continuum. These discrepancies further transpired to reflect crucial differential experiences along gender lines and income levels in each site. Vulnerability was often context specific, not only because of unique drivers of stress in different areas, but also because socio-economic groups and localities often had characteristics that could potentially exacerbate vulnerability, as well as characteristics that can potentially facilitate adaptive capacity. Stressors were found to have depleted multiple forms of capital over time, while new stressors were emerging, raising concerns over the most appropriate means of social protection within these contexts.
- Full Text:
- Date Issued: 2013
- Authors: Stadler, Leigh Tessa
- Date: 2013
- Subjects: Climatic changes -- South Africa -- Eastern Cape Climatic changes -- Health aspects -- South Africa -- Eastern Cape Climatic changes -- Economic aspects -- South Africa -- Eastern Cape HIV infections -- Economic aspects -- South Africa -- Eastern Cape AIDS (Disease) -- Economic aspects -- South Africa -- Eastern Cape HIV infections -- Social aspects -- South Africa -- Eastern Cape AIDS (Disease) -- Social aspects -- South Africa -- Eastern Cape Poverty -- Social aspects -- South Africa -- Eastern Cape Income -- South Africa -- Eastern Cape Human capital -- South Africa -- Eastern Cape Sex role -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4726 , http://hdl.handle.net/10962/d1001655
- Description: Livelihood stressors in southern Africa, such as HIV/Aids and climate change, do not act in isolation but rather interact concurrently in complex socio-ecological systems with diverse, interrelated and compounded affects. Households experience differential vulnerability to such stressors based on contextual factors such as geographical location, income level and the gender and age of its members. Households’ differential experiences of vulnerability are further defined by the households’ use of their capital stocks: the human, social, natural, financial and physical capital available to the household to form livelihoods and resist the detrimental effects of a stressor. The capital stocks of 340 households were measured in two sites in the Eastern Cape, South Africa, using a household survey. These data were analysed to determine differences between the sites, households with heads of different gender and households of different income levels. Further data relating to the drivers and interactions of stressors over temporal and spatial scales, as well as the perceived value of various forms of capital by different social groups in the two sites, were collected via Participatory Learning and Action (PLA) methods including timelines, mental modelling and pair-wise ranking. Although the two sites have similar levels of income and fall within the same province, many significant differences emerged. The two sites showed different distributions of household head genders and different stressors and perceptions of vulnerability, perhaps owing to differences in their capital stocks, acting alongside the influence of culture and access on a shifting rural-urban continuum. These discrepancies further transpired to reflect crucial differential experiences along gender lines and income levels in each site. Vulnerability was often context specific, not only because of unique drivers of stress in different areas, but also because socio-economic groups and localities often had characteristics that could potentially exacerbate vulnerability, as well as characteristics that can potentially facilitate adaptive capacity. Stressors were found to have depleted multiple forms of capital over time, while new stressors were emerging, raising concerns over the most appropriate means of social protection within these contexts.
- Full Text:
- Date Issued: 2013
Barriers to public participation in developmental projects : a Nigerian community perspective (case study of the Gbaran-Ubie oil and gas project)
- Authors: Akoh, Emmanuel Inalegwu
- Date: 2013
- Subjects: Gbaran-Ubie oil and gas project -- Public opinion Community development -- Nigeria Economic development -- Nigeria -- Environmental aspects
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:715 , http://hdl.handle.net/10962/d1001869
- Description: This research paper is broken up into three sections, namely an evaluation report, a literature review and research methodology. The evaluation report section is the assessment of the current state of change readiness within the Eskom Contact Centre’s based upon what the literature on change readiness prescribes. The timing of the change readiness assessment is just prior to significant organisational change. The organisational change that was about to be initiated by the organisation was initiated from the boardroom of the most senior echelons of the organisation, and was directed in a top down approach, being a strategic organisational change. The change is deemed to be critical to the organisation being able to meet its long term strategic and sustainability objectives. A critical examination of the literature explored the meaning of change readiness, the importance of it and explained the consequences for organisations that commit to transformational agendas without being ready. Key concepts such as such resistance to change and organisational inertia are described and differentiated from change readiness. The ADKAR change model and its change readiness assessment instrument were used due to the organisations preference for the model. The ADKAR model formed the framework for the analyses of the data, the discussion of the results and the recommendations to the organisation. The research conducted was quantitative in nature; a questionnaire was distributed to the employees of the seven Eskom Contact centre sites around the country through an email. A slightly modified version of the ADKAR change readiness questionnaire was sent via email with an on-line questionnaire link on it; and questions on individual readiness for change were used to assess the level of readiness of the employees. Most of the descriptive and inferential statistics were analysed with the use of Excel (version, 2010), with Factor Analysis being done in Statistica. The results of the research showed that: [iii] - The factors as proposed by the ADKAR change readiness assessment questionnaire (i.e. Awareness, Desire, Knowledge, Ability and Reinforcement) are not different enough to be considered as independent factors for this data set. Based on factor analysis, the factors were subsequently amended from five to three, namely Readiness, Opportunity Realisation and Uncertainty. - The Contact Centre employees were somewhat ready for change. - The Contact Centres needed to focus on all amended ADKAR factors in order to improve the readiness of the department. - The readiness levels in response to the roll out were more or less uniform. The study shows that given Eskom’s preference for the ADKAR model, future research within Eskom should therefore be conducted more circumspectly with respect to ascertaining the validity of the ADKAR factors. The study also makes mention that future work and/or research will need to be conducted, specifically on the readiness of the organisation itself, in order to improve the probability of transformational success. The ADKAR assessment is a people focused assessment and therefore focuses only on the readiness of the individual. Factors such as the adequacy of the current state of resources within the organisation, which incorporate aspects such as infrastructure, technology and staffing, will also need to be assessed to make a more holistic statement of change readiness. A concise review of the literature is incorporated into the Evaluation Report of Section 1 to underpin the study. In Section 2 a more extensive review of the literature is presented. Similarly, the design of the research is discussed in more detail in Section 3 to both describe and justify the appropriateness of the research methodology, and to give a detailed account of the way in which the research was carried out.
- Full Text:
- Date Issued: 2013
- Authors: Akoh, Emmanuel Inalegwu
- Date: 2013
- Subjects: Gbaran-Ubie oil and gas project -- Public opinion Community development -- Nigeria Economic development -- Nigeria -- Environmental aspects
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:715 , http://hdl.handle.net/10962/d1001869
- Description: This research paper is broken up into three sections, namely an evaluation report, a literature review and research methodology. The evaluation report section is the assessment of the current state of change readiness within the Eskom Contact Centre’s based upon what the literature on change readiness prescribes. The timing of the change readiness assessment is just prior to significant organisational change. The organisational change that was about to be initiated by the organisation was initiated from the boardroom of the most senior echelons of the organisation, and was directed in a top down approach, being a strategic organisational change. The change is deemed to be critical to the organisation being able to meet its long term strategic and sustainability objectives. A critical examination of the literature explored the meaning of change readiness, the importance of it and explained the consequences for organisations that commit to transformational agendas without being ready. Key concepts such as such resistance to change and organisational inertia are described and differentiated from change readiness. The ADKAR change model and its change readiness assessment instrument were used due to the organisations preference for the model. The ADKAR model formed the framework for the analyses of the data, the discussion of the results and the recommendations to the organisation. The research conducted was quantitative in nature; a questionnaire was distributed to the employees of the seven Eskom Contact centre sites around the country through an email. A slightly modified version of the ADKAR change readiness questionnaire was sent via email with an on-line questionnaire link on it; and questions on individual readiness for change were used to assess the level of readiness of the employees. Most of the descriptive and inferential statistics were analysed with the use of Excel (version, 2010), with Factor Analysis being done in Statistica. The results of the research showed that: [iii] - The factors as proposed by the ADKAR change readiness assessment questionnaire (i.e. Awareness, Desire, Knowledge, Ability and Reinforcement) are not different enough to be considered as independent factors for this data set. Based on factor analysis, the factors were subsequently amended from five to three, namely Readiness, Opportunity Realisation and Uncertainty. - The Contact Centre employees were somewhat ready for change. - The Contact Centres needed to focus on all amended ADKAR factors in order to improve the readiness of the department. - The readiness levels in response to the roll out were more or less uniform. The study shows that given Eskom’s preference for the ADKAR model, future research within Eskom should therefore be conducted more circumspectly with respect to ascertaining the validity of the ADKAR factors. The study also makes mention that future work and/or research will need to be conducted, specifically on the readiness of the organisation itself, in order to improve the probability of transformational success. The ADKAR assessment is a people focused assessment and therefore focuses only on the readiness of the individual. Factors such as the adequacy of the current state of resources within the organisation, which incorporate aspects such as infrastructure, technology and staffing, will also need to be assessed to make a more holistic statement of change readiness. A concise review of the literature is incorporated into the Evaluation Report of Section 1 to underpin the study. In Section 2 a more extensive review of the literature is presented. Similarly, the design of the research is discussed in more detail in Section 3 to both describe and justify the appropriateness of the research methodology, and to give a detailed account of the way in which the research was carried out.
- Full Text:
- Date Issued: 2013
Biochemical characterisation of Pfj2, a Plasmodium falciparum heat shock protein 40 chaperone potentially involved in protein quality control in the endoplasmic reticulum
- Authors: Afolayan, Omolola Folasade
- Date: 2013
- Subjects: Plasmodium falciparum Endoplasmic reticulum Heat shock proteins Malaria , Mosquito-borne infectious disease
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3883 , http://hdl.handle.net/10962/d1001617
- Description: Plasmodium falciparum is a protozoan parasite that causes a severe form of malaria, a mosquito-borne infectious disease in humans. P. falciparum encodes a number of proteins to facilitate its life-cycle, including a type II heat shock protein 40 (Hsp40), Pfj2. Pfj2 shows a degree of homology to human ERdj5, a resident protein of the endoplasmic reticulum (ER) that promotes protein quality control by facilitating the degradation of misfolded proteins. The overall aim of this study was to further understand the function of Pfj2 in the P. falciparum cell by characterising it biochemically. A bioinformatic analysis of Pfj2 was carried out to enable the identification of a potential ER signal sequence and cleavage site. Furthermore, an analysis of Pfj2 protein sequence was performed to compare domain similarities and identities with typical type II Hsp40s namely, human ERdj5, S. cerevisiae Sis1, human Hsj1a and human DnaJB4. The method used included the insertion of the codon-optimised coding sequence for the processed ER form of Pfj2 into the prokaryotic expression vector, pQE30, to enable overproduction of a histidine-tagged protein. A 62 kDa His₆-Pfj2 was successfully expressed in Escherichia coli and purified using denaturing nickel affinity chromatography. ATPase assays were performed to determine the ability of His₆- Pfj2 to stimulate the chaperone activity of the ER Hsp70, also called immunoglobulin binding protein (BiP). Initial studies were conducted on readily available mammalian His₆-BiP as a control, which was shown to have an intrinsic activity of 12.07±3.92 nmolPi/min/mg. His₆- Pfj2 did not stimulate the ATPase activity of mammalian His₆-BiP, suggesting that it either could not act as a co-chaperone of mammalian His₆-BiP (specificity), or it required a misfolded substrate in the system. Therefore, ongoing studies are addressing the interaction of Pfj2 and misfolded substrates with P. falciparum BiP. The results of these studies will further our understanding of a poorly-studied parasite chaperone that represents a potential drug target for development of novel strategies for the control of a serious human disease
- Full Text:
- Date Issued: 2013
- Authors: Afolayan, Omolola Folasade
- Date: 2013
- Subjects: Plasmodium falciparum Endoplasmic reticulum Heat shock proteins Malaria , Mosquito-borne infectious disease
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3883 , http://hdl.handle.net/10962/d1001617
- Description: Plasmodium falciparum is a protozoan parasite that causes a severe form of malaria, a mosquito-borne infectious disease in humans. P. falciparum encodes a number of proteins to facilitate its life-cycle, including a type II heat shock protein 40 (Hsp40), Pfj2. Pfj2 shows a degree of homology to human ERdj5, a resident protein of the endoplasmic reticulum (ER) that promotes protein quality control by facilitating the degradation of misfolded proteins. The overall aim of this study was to further understand the function of Pfj2 in the P. falciparum cell by characterising it biochemically. A bioinformatic analysis of Pfj2 was carried out to enable the identification of a potential ER signal sequence and cleavage site. Furthermore, an analysis of Pfj2 protein sequence was performed to compare domain similarities and identities with typical type II Hsp40s namely, human ERdj5, S. cerevisiae Sis1, human Hsj1a and human DnaJB4. The method used included the insertion of the codon-optimised coding sequence for the processed ER form of Pfj2 into the prokaryotic expression vector, pQE30, to enable overproduction of a histidine-tagged protein. A 62 kDa His₆-Pfj2 was successfully expressed in Escherichia coli and purified using denaturing nickel affinity chromatography. ATPase assays were performed to determine the ability of His₆- Pfj2 to stimulate the chaperone activity of the ER Hsp70, also called immunoglobulin binding protein (BiP). Initial studies were conducted on readily available mammalian His₆-BiP as a control, which was shown to have an intrinsic activity of 12.07±3.92 nmolPi/min/mg. His₆- Pfj2 did not stimulate the ATPase activity of mammalian His₆-BiP, suggesting that it either could not act as a co-chaperone of mammalian His₆-BiP (specificity), or it required a misfolded substrate in the system. Therefore, ongoing studies are addressing the interaction of Pfj2 and misfolded substrates with P. falciparum BiP. The results of these studies will further our understanding of a poorly-studied parasite chaperone that represents a potential drug target for development of novel strategies for the control of a serious human disease
- Full Text:
- Date Issued: 2013
Biology and ecology of largemouth bass Micropterus salmoides in two temperate South African impoundments
- Authors: Taylor, Geraldine Claire
- Date: 2013 , 2013-03-19
- Subjects: Largemouth bass -- Ecology -- South Africa -- Eastern Cape , Largemouth bass -- Behavior -- South Africa -- Eastern Cape , Largemouth bass -- Growth -- South Africa -- Eastern Cape , Largemouth bass -- Reproduction -- South Africa -- Eastern Cape , Largemouth bass -- Food -- South Africa -- Eastern Cape , Largemouth bass -- Age determination -- South Africa -- Eastern Cape , Introduced fishes -- South Africa -- Eastern Cape , Fish populations -- South Africa -- Eastern Cape , Otoliths , Largemouth bass -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5181 , http://hdl.handle.net/10962/d1001668 , Largemouth bass -- Ecology -- South Africa -- Eastern Cape , Largemouth bass -- Behavior -- South Africa -- Eastern Cape , Largemouth bass -- Growth -- South Africa -- Eastern Cape , Largemouth bass -- Reproduction -- South Africa -- Eastern Cape , Largemouth bass -- Food -- South Africa -- Eastern Cape , Largemouth bass -- Age determination -- South Africa -- Eastern Cape , Introduced fishes -- South Africa -- Eastern Cape , Fish populations -- South Africa -- Eastern Cape , Otoliths
- Description: Globally largemouth bass Micropterus salmoides is one of the most widely introduced game fish species which has now become invasive in many countries. Well researched in its native North America, there are few studies on this species in its introduced range. This study aims to improve the understanding of the biology and ecology of M. salmoides in temperate southern Africa, where it threatens native biota and supports a popular recreational fishery. This aim was addressed by assessing the age, growth, maturity, reproductive seasonality, feeding, mortality and movement, of two M. salmoides populations from Wriggleswade (1000 ha, 723 m amsl, 32º35'S; 27º33'E) and Mankazana (35 ha, 66 m amsl, 33º09'S; 26º57'E) Dams in the Eastern Cape of South Africa. The most suitable structure for ageing M. salmoides was determined by comparing the accuracy and precision of otoliths and scales. Scales tended to underestimate the age of fish older than five years. Sectioned sagittal otoliths were the more precise structures for ageing (otoliths CV = 15.8 vs. scales CV = 21.9). The periodicity of growth zone formation was validated as annual for otoliths using both edge analysis (EA), and mark recapture of chemically tagged fish (MRCT). EA indicated one annual peak in the frequency of opaque margins between September and January using a periodic logistic regression and a binomial model linked with a von Mises distribution for circular data. MRCT showed the linear relationship between time at liberty and number of growth zones distal to the fluorescent band was not significantly different from one (slope = 0.89). Reproduction was similar to that in native and non-native populations with fish reaching age at 50% maturity at ca. two years, and length at 50% maturity at 259-290 mm FL depending on growth rate. Spawning season occurred in the spring months of August to October. The Wriggleswade Dam population reached similar ages (14 yrs) to populations in temperate North America. Growth was described using the von Bertalanffy growth equations of (Lt = 420(1-ᵉ⁻°·³³⁽ᵗ⁺°·²¹⁾) mm FL in Wriggleswade and (Lt = 641(1-ᵉ⁻°·²²⁽ᵗ⁺°·⁷⁶⁾) mm FL in Mankazana. Growth performance of both populations, described using the phi prime index, seemed to be correlated with temperature and the Wriggleswade Dam population (Φ’ = 2.8) grew similarly to those native temperate North American populations and the non-native populations of Japan and Italy. The Mankazana population had a higher growth performance (Φ’ = 2.9) compared to Wriggleswade and growth was comparable to other nonnative African populations. Both populations utilised all available food resources, with the Mankazana population being in better condition than the Wriggleswade population as a result of a more diverse diet, highly abundant food sources throughout the year, and potentially the adoption of a more conservative ambush feeding strategy. The Wriggleswade population, which was utilised by competitive anglers, showed low mortality rates (Z = 0.43 yr⁻¹), and translocated fish dispersed from the common release site, travelling an average distance of 4km over the study time of 494 days. These results indicated that M. salmoides are successful in these environments, growing at expected temperature dependant rates, maturing at two years, using all available food groups, and suffering from low mortality. It is therefore likely that they will persist in South Africa and establish where introduced.
- Full Text:
- Date Issued: 2013
- Authors: Taylor, Geraldine Claire
- Date: 2013 , 2013-03-19
- Subjects: Largemouth bass -- Ecology -- South Africa -- Eastern Cape , Largemouth bass -- Behavior -- South Africa -- Eastern Cape , Largemouth bass -- Growth -- South Africa -- Eastern Cape , Largemouth bass -- Reproduction -- South Africa -- Eastern Cape , Largemouth bass -- Food -- South Africa -- Eastern Cape , Largemouth bass -- Age determination -- South Africa -- Eastern Cape , Introduced fishes -- South Africa -- Eastern Cape , Fish populations -- South Africa -- Eastern Cape , Otoliths , Largemouth bass -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5181 , http://hdl.handle.net/10962/d1001668 , Largemouth bass -- Ecology -- South Africa -- Eastern Cape , Largemouth bass -- Behavior -- South Africa -- Eastern Cape , Largemouth bass -- Growth -- South Africa -- Eastern Cape , Largemouth bass -- Reproduction -- South Africa -- Eastern Cape , Largemouth bass -- Food -- South Africa -- Eastern Cape , Largemouth bass -- Age determination -- South Africa -- Eastern Cape , Introduced fishes -- South Africa -- Eastern Cape , Fish populations -- South Africa -- Eastern Cape , Otoliths
- Description: Globally largemouth bass Micropterus salmoides is one of the most widely introduced game fish species which has now become invasive in many countries. Well researched in its native North America, there are few studies on this species in its introduced range. This study aims to improve the understanding of the biology and ecology of M. salmoides in temperate southern Africa, where it threatens native biota and supports a popular recreational fishery. This aim was addressed by assessing the age, growth, maturity, reproductive seasonality, feeding, mortality and movement, of two M. salmoides populations from Wriggleswade (1000 ha, 723 m amsl, 32º35'S; 27º33'E) and Mankazana (35 ha, 66 m amsl, 33º09'S; 26º57'E) Dams in the Eastern Cape of South Africa. The most suitable structure for ageing M. salmoides was determined by comparing the accuracy and precision of otoliths and scales. Scales tended to underestimate the age of fish older than five years. Sectioned sagittal otoliths were the more precise structures for ageing (otoliths CV = 15.8 vs. scales CV = 21.9). The periodicity of growth zone formation was validated as annual for otoliths using both edge analysis (EA), and mark recapture of chemically tagged fish (MRCT). EA indicated one annual peak in the frequency of opaque margins between September and January using a periodic logistic regression and a binomial model linked with a von Mises distribution for circular data. MRCT showed the linear relationship between time at liberty and number of growth zones distal to the fluorescent band was not significantly different from one (slope = 0.89). Reproduction was similar to that in native and non-native populations with fish reaching age at 50% maturity at ca. two years, and length at 50% maturity at 259-290 mm FL depending on growth rate. Spawning season occurred in the spring months of August to October. The Wriggleswade Dam population reached similar ages (14 yrs) to populations in temperate North America. Growth was described using the von Bertalanffy growth equations of (Lt = 420(1-ᵉ⁻°·³³⁽ᵗ⁺°·²¹⁾) mm FL in Wriggleswade and (Lt = 641(1-ᵉ⁻°·²²⁽ᵗ⁺°·⁷⁶⁾) mm FL in Mankazana. Growth performance of both populations, described using the phi prime index, seemed to be correlated with temperature and the Wriggleswade Dam population (Φ’ = 2.8) grew similarly to those native temperate North American populations and the non-native populations of Japan and Italy. The Mankazana population had a higher growth performance (Φ’ = 2.9) compared to Wriggleswade and growth was comparable to other nonnative African populations. Both populations utilised all available food resources, with the Mankazana population being in better condition than the Wriggleswade population as a result of a more diverse diet, highly abundant food sources throughout the year, and potentially the adoption of a more conservative ambush feeding strategy. The Wriggleswade population, which was utilised by competitive anglers, showed low mortality rates (Z = 0.43 yr⁻¹), and translocated fish dispersed from the common release site, travelling an average distance of 4km over the study time of 494 days. These results indicated that M. salmoides are successful in these environments, growing at expected temperature dependant rates, maturing at two years, using all available food groups, and suffering from low mortality. It is therefore likely that they will persist in South Africa and establish where introduced.
- Full Text:
- Date Issued: 2013
Case study : using visual representations to enhance conceptual knowledge of division in mathematics
- Authors: Joel, Linea Beautty
- Date: 2013
- Subjects: Mathematics -- Study and teaching , Visual learning , Division -- Study and teaching
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1992 , http://hdl.handle.net/10962/d1013356
- Description: Literature emphasizes how important it is that procedural and conceptual knowledge of mathematics should be learned in integration. Yet, generally, the learning and teaching in mathematics classrooms relies heavily on isolated procedures. This study aims to improve teaching and learning of partitive and quotitive division, moving away from isolated procedural knowledge to that of procedures with their underlying concepts through the use of manipulatives, visual representation and questioning. Learning and teaching lessons were designed to teach partitive and quotitive division both procedurally and conceptually. The study explored the roles these manipulatives, visual representations and questioning played toward the conceptual learning of partitive and quotitive division. It was found that manipulatives and iconic visualization enhanced learning, and this could be achieved through scaffolding using a questioning approach. It was concluded that manipulatives and iconic visualization need to be properly planned and used, and integrated with questioning to achieve success in the learning of procedural and conceptual knowledge.
- Full Text:
- Date Issued: 2013
Case study : using visual representations to enhance conceptual knowledge of division in mathematics
- Authors: Joel, Linea Beautty
- Date: 2013
- Subjects: Mathematics -- Study and teaching , Visual learning , Division -- Study and teaching
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1992 , http://hdl.handle.net/10962/d1013356
- Description: Literature emphasizes how important it is that procedural and conceptual knowledge of mathematics should be learned in integration. Yet, generally, the learning and teaching in mathematics classrooms relies heavily on isolated procedures. This study aims to improve teaching and learning of partitive and quotitive division, moving away from isolated procedural knowledge to that of procedures with their underlying concepts through the use of manipulatives, visual representation and questioning. Learning and teaching lessons were designed to teach partitive and quotitive division both procedurally and conceptually. The study explored the roles these manipulatives, visual representations and questioning played toward the conceptual learning of partitive and quotitive division. It was found that manipulatives and iconic visualization enhanced learning, and this could be achieved through scaffolding using a questioning approach. It was concluded that manipulatives and iconic visualization need to be properly planned and used, and integrated with questioning to achieve success in the learning of procedural and conceptual knowledge.
- Full Text:
- Date Issued: 2013
Changes in muscle recruitment, functional strength and ratings of perceived effort during an 8-over bowling spell: impact on performance
- Authors: Barford, Gareth Charles
- Date: 2013
- Subjects: Cricket -- Bowling , Sports -- Physiological aspects , Muscles -- Wounds and injuries , Fatigue , Physical fitness , Cricket injuries
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5102 , http://hdl.handle.net/10962/d1003926 , Cricket -- Bowling , Sports -- Physiological aspects , Muscles -- Wounds and injuries , Fatigue , Physical fitness , Cricket injuries
- Description: Background: The musculoskeletal demands placed on the lower limb musculature of fast bowlers over time have not received much attention. In particular, measures of muscle recruitment changes have, to the author’s knowledge, not been considered. Objective: The present study, therefore sought to establish any associations between an eight over, simulated fast bowling spell, and muscle activation patterns, power output, perceptual demands, and changes in performance. This will enable improvements in the development of training programmes. Methods: Players’ were required to attend two sessions in total. The purpose of the initial session was to collect specific demographic, anthropometric and physiological data and injury history information from each player. In addition, this first session allowed for habituation with the treadmill, the jump meter and all other equipment involved in experimentation. The second testing session involved electrode attachment sites being identified on player’s dominant leg. The areas were then shaved, wiped with an alcohol swab and left to dry, to ensure good connectivity. Pre- and post- measures of muscle activity and functional strength of the lower limbs were recorded in the Department of Human Kinetics and Ergonomics. The protocol took place at the Kingswood High Performance Centre, which is in close proximity to the initial testing site. The protocol involved players bowling eight overs (48 balls). During the protocol, accuracy, ball release speed and perceptual measures were recorded at the end of each over. After the protocol, players were driven back to the Human Kinetics and Ergonomics Department where post-testing measures were completed. The dependable variables of interest were muscle activation, functional strength of the lower limbs, ‘local’ ratings of perceived exertion (RPE), body discomfort, accuracy, and ball release speed. Results: For all muscles it was shown that, as the speed increased so did the muscle activity in players’ lower limbs. There were no significant changes in muscle activity preversus post-protocol. There was however, a general trend of decreasing muscle activity post protocol at higher testing speeds. There were significant (p<0.05) decreases in peak power following the simulated eight over bowling spell. ‘Local’ RPE displayed a significant (P<0.05) increase with each additional over and were observed to reach the ‘heavy’ category. The players’ highest discomfort area was in the lower back, with 13 players perceiving discomfort in this region following the eight over spell. The shoulder and chest were another two areas player’s indicated discomfort with eight players selecting the dominant shoulder. Seven players complained of the dominant side pectoral muscle, leading foot and dominant latissimus dorsi muscle being uncomfortable. Interestingly, the dominant pectoral showed the highest body discomfort ratings amongst players. There were no significant changes in accuracy between overs although there were large interindividual differences in accuracy points between players. The decrease in ball release speed observed during over seven was shown to be significantly (p<0.05) lower than overs one to four. Conclusion: The power output and perceived strain results of the players, appears to indicate the presence of fatigue in players. However, the results are not conclusive, as the fatigue was not shown in muscle recruitment patterns, as well as the body discomfort ratings. There was a non-significant trend observed in the lower limb muscle activation decreasing at higher speeds. Players were able to maintain accuracy. However, the significantly lower ball release speed observed during over seven showed players performance decreasing.
- Full Text:
- Date Issued: 2013
- Authors: Barford, Gareth Charles
- Date: 2013
- Subjects: Cricket -- Bowling , Sports -- Physiological aspects , Muscles -- Wounds and injuries , Fatigue , Physical fitness , Cricket injuries
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5102 , http://hdl.handle.net/10962/d1003926 , Cricket -- Bowling , Sports -- Physiological aspects , Muscles -- Wounds and injuries , Fatigue , Physical fitness , Cricket injuries
- Description: Background: The musculoskeletal demands placed on the lower limb musculature of fast bowlers over time have not received much attention. In particular, measures of muscle recruitment changes have, to the author’s knowledge, not been considered. Objective: The present study, therefore sought to establish any associations between an eight over, simulated fast bowling spell, and muscle activation patterns, power output, perceptual demands, and changes in performance. This will enable improvements in the development of training programmes. Methods: Players’ were required to attend two sessions in total. The purpose of the initial session was to collect specific demographic, anthropometric and physiological data and injury history information from each player. In addition, this first session allowed for habituation with the treadmill, the jump meter and all other equipment involved in experimentation. The second testing session involved electrode attachment sites being identified on player’s dominant leg. The areas were then shaved, wiped with an alcohol swab and left to dry, to ensure good connectivity. Pre- and post- measures of muscle activity and functional strength of the lower limbs were recorded in the Department of Human Kinetics and Ergonomics. The protocol took place at the Kingswood High Performance Centre, which is in close proximity to the initial testing site. The protocol involved players bowling eight overs (48 balls). During the protocol, accuracy, ball release speed and perceptual measures were recorded at the end of each over. After the protocol, players were driven back to the Human Kinetics and Ergonomics Department where post-testing measures were completed. The dependable variables of interest were muscle activation, functional strength of the lower limbs, ‘local’ ratings of perceived exertion (RPE), body discomfort, accuracy, and ball release speed. Results: For all muscles it was shown that, as the speed increased so did the muscle activity in players’ lower limbs. There were no significant changes in muscle activity preversus post-protocol. There was however, a general trend of decreasing muscle activity post protocol at higher testing speeds. There were significant (p<0.05) decreases in peak power following the simulated eight over bowling spell. ‘Local’ RPE displayed a significant (P<0.05) increase with each additional over and were observed to reach the ‘heavy’ category. The players’ highest discomfort area was in the lower back, with 13 players perceiving discomfort in this region following the eight over spell. The shoulder and chest were another two areas player’s indicated discomfort with eight players selecting the dominant shoulder. Seven players complained of the dominant side pectoral muscle, leading foot and dominant latissimus dorsi muscle being uncomfortable. Interestingly, the dominant pectoral showed the highest body discomfort ratings amongst players. There were no significant changes in accuracy between overs although there were large interindividual differences in accuracy points between players. The decrease in ball release speed observed during over seven was shown to be significantly (p<0.05) lower than overs one to four. Conclusion: The power output and perceived strain results of the players, appears to indicate the presence of fatigue in players. However, the results are not conclusive, as the fatigue was not shown in muscle recruitment patterns, as well as the body discomfort ratings. There was a non-significant trend observed in the lower limb muscle activation decreasing at higher speeds. Players were able to maintain accuracy. However, the significantly lower ball release speed observed during over seven showed players performance decreasing.
- Full Text:
- Date Issued: 2013
Characterisation of a plasmodium falciparum type II Hsp40 chaperone exported to the cytosol of infected erythrocytes
- Maphumulo, Philile Nompumelelo
- Authors: Maphumulo, Philile Nompumelelo
- Date: 2013
- Subjects: Erythrocytes , Heat shock proteins , Plasmodium falciparum , Molecular chaperones , Malaria -- Prevention -- Research , Protein folding , Proteins -- Analysis , Malaria -- Immunological aspects
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4128 , http://hdl.handle.net/10962/d1015681
- Description: Heat Shock 40 kDa proteins (Hsp40s) partner with heat shock 70 kDa proteins (Hsp70s) in facilitating, among other chaperone activities; correct protein transport, productive protein folding and assembly within the cells; under both normal and stressful conditions. Hsp40 proteins regulate the ATPase activity of Hsp70 through interaction with the J-domain. Plasmodium falciparum Hsp70s (PfHsp70s) do not contain a Plasmodium export element (PEXEL) sequence although PfHsp70-1 and PfHsp70-3 have been located outside of the parasitophorous vacuole. Studies reveal that a type I P. falciparum (PfHsp40) chaperone (PF14_0359) stimulates the rate of ATP hydrolysis of the cytosolic PfHsp70 (PfHsp70-1) and that of human Hsp70A1A. PFE0055c is a PEXEL-bearing type II Hsp40 that is exported into the cytosol of P. falciparum-infected erythrocytes; where it potentially interacts with human Hsp70. Studies reveal that PFE0055c associates with structures found in the erythrocyte cytosol termed “J-dots” which are believed to be involved in trafficking parasite-encoded proteins through the erythrocyte cytosol. If P. falciparum exports PFE0055c into the host cytosol, it may be proposed that it interacts with human Hsp70, making it a possible drug target. The effect of PFE0055c on the ATPase activity of human Hsp70A1A has not been previously characterised. Central to this study was bioinformatic analysis and biochemical characterisation PFE0055c using an in vitro (ATPase assay) approach. Structural domains that classify PFE0055c as a type II Hsp40 were identified with similarity to two other exported type II PfHsp40s. Plasmids encoding the hexahistidine-tagged versions of PFE0055c and human Hsp70A1A were used for the expression and purification of these proteins from Escherichia coli. Purification was achieved using nickel affinity chromatography. The urea-denaturing method was used to obtain the purified PFE0055c whilst human Hsp70A1A was purified using the native method. PFE0055c could stimulate the ATPase activity of alfalfa Hsp70, although such was not the case for human Hsp70A1A in vitro.
- Full Text:
- Date Issued: 2013
- Authors: Maphumulo, Philile Nompumelelo
- Date: 2013
- Subjects: Erythrocytes , Heat shock proteins , Plasmodium falciparum , Molecular chaperones , Malaria -- Prevention -- Research , Protein folding , Proteins -- Analysis , Malaria -- Immunological aspects
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4128 , http://hdl.handle.net/10962/d1015681
- Description: Heat Shock 40 kDa proteins (Hsp40s) partner with heat shock 70 kDa proteins (Hsp70s) in facilitating, among other chaperone activities; correct protein transport, productive protein folding and assembly within the cells; under both normal and stressful conditions. Hsp40 proteins regulate the ATPase activity of Hsp70 through interaction with the J-domain. Plasmodium falciparum Hsp70s (PfHsp70s) do not contain a Plasmodium export element (PEXEL) sequence although PfHsp70-1 and PfHsp70-3 have been located outside of the parasitophorous vacuole. Studies reveal that a type I P. falciparum (PfHsp40) chaperone (PF14_0359) stimulates the rate of ATP hydrolysis of the cytosolic PfHsp70 (PfHsp70-1) and that of human Hsp70A1A. PFE0055c is a PEXEL-bearing type II Hsp40 that is exported into the cytosol of P. falciparum-infected erythrocytes; where it potentially interacts with human Hsp70. Studies reveal that PFE0055c associates with structures found in the erythrocyte cytosol termed “J-dots” which are believed to be involved in trafficking parasite-encoded proteins through the erythrocyte cytosol. If P. falciparum exports PFE0055c into the host cytosol, it may be proposed that it interacts with human Hsp70, making it a possible drug target. The effect of PFE0055c on the ATPase activity of human Hsp70A1A has not been previously characterised. Central to this study was bioinformatic analysis and biochemical characterisation PFE0055c using an in vitro (ATPase assay) approach. Structural domains that classify PFE0055c as a type II Hsp40 were identified with similarity to two other exported type II PfHsp40s. Plasmids encoding the hexahistidine-tagged versions of PFE0055c and human Hsp70A1A were used for the expression and purification of these proteins from Escherichia coli. Purification was achieved using nickel affinity chromatography. The urea-denaturing method was used to obtain the purified PFE0055c whilst human Hsp70A1A was purified using the native method. PFE0055c could stimulate the ATPase activity of alfalfa Hsp70, although such was not the case for human Hsp70A1A in vitro.
- Full Text:
- Date Issued: 2013
Characterisation of surfaces modified through self-assembled monolayers and click chemistry
- Authors: Coates, Megan Patricia
- Date: 2013
- Subjects: Monomolecular films Gold Adsorption Nanotubes Self-assembly (Chemistry) Self-assembly (Chemistry) Scanning electrochemical microscopy X-ray photoelectron spectroscopy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4277 , http://hdl.handle.net/10962/d1001684
- Description: Different approaches to surface modification were investigated in this work on gold, glassy carbon, multi-walled carbon nanotube paper and on single-walled carbon nanotubes adsorbed on glassy carbon. These approaches include electrochemical grafting, electropolymerisation, click chemistry, axial ligation, adsorption and self-assembled monolayers. The modified surfaces were characterised using a variety of techniques; predominantly electrochemistry, scanning electrochemical microscopy and X-ray photoelectron spectroscopy. For the formation of self-assembled monolayers on gold, four new manganese(III) phthalocyanines (1a-d), octa-substituted at the peripheral position with pentylthio, decylthio, benzylthio, and phenylthio groups were synthesized and characterised. X-ray photoelectron spectroscopy was used to show the formation of a sulphur-gold bond. A number of approaches using 4-azidoaniline (2a) combined with azide-alkyne click chemistry and electrochemistry were also used to anchor ferrocene and pyridine moieties on to the carbon surfaces, including direct in situ diazotation and grafting, electropolymerisation, and the synthesis of the diazonium salt followed by grafting. Iron phthalocyanine was linked to the pyridine-clicked surfaces through axial ligation, where the strong axial bond formed by the interaction between the central metal and the lone pair of the nitrogen in the pyridine group resulted in stable modified electrodes. The potential of these surfaces for the detection of analytes such as thiocyanate, hydrazine and sulphite are briefly shown as well. This work also describes for the first time the possibility of performing local micro-electrochemical grafting of a gold substrate by 4-azidobenzenediazonium (2b) using scanning electrochemical microscopy in a single and simple one step approach, without complications from adsorption.
- Full Text:
- Date Issued: 2013
- Authors: Coates, Megan Patricia
- Date: 2013
- Subjects: Monomolecular films Gold Adsorption Nanotubes Self-assembly (Chemistry) Self-assembly (Chemistry) Scanning electrochemical microscopy X-ray photoelectron spectroscopy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4277 , http://hdl.handle.net/10962/d1001684
- Description: Different approaches to surface modification were investigated in this work on gold, glassy carbon, multi-walled carbon nanotube paper and on single-walled carbon nanotubes adsorbed on glassy carbon. These approaches include electrochemical grafting, electropolymerisation, click chemistry, axial ligation, adsorption and self-assembled monolayers. The modified surfaces were characterised using a variety of techniques; predominantly electrochemistry, scanning electrochemical microscopy and X-ray photoelectron spectroscopy. For the formation of self-assembled monolayers on gold, four new manganese(III) phthalocyanines (1a-d), octa-substituted at the peripheral position with pentylthio, decylthio, benzylthio, and phenylthio groups were synthesized and characterised. X-ray photoelectron spectroscopy was used to show the formation of a sulphur-gold bond. A number of approaches using 4-azidoaniline (2a) combined with azide-alkyne click chemistry and electrochemistry were also used to anchor ferrocene and pyridine moieties on to the carbon surfaces, including direct in situ diazotation and grafting, electropolymerisation, and the synthesis of the diazonium salt followed by grafting. Iron phthalocyanine was linked to the pyridine-clicked surfaces through axial ligation, where the strong axial bond formed by the interaction between the central metal and the lone pair of the nitrogen in the pyridine group resulted in stable modified electrodes. The potential of these surfaces for the detection of analytes such as thiocyanate, hydrazine and sulphite are briefly shown as well. This work also describes for the first time the possibility of performing local micro-electrochemical grafting of a gold substrate by 4-azidobenzenediazonium (2b) using scanning electrochemical microscopy in a single and simple one step approach, without complications from adsorption.
- Full Text:
- Date Issued: 2013
Characterization and application of phthalocyanine-gold nanoparticle conjugates
- Authors: Tombe, Sekai Lana
- Date: 2013
- Subjects: Phthalocyanines , Gold , Zinc , Nanoparticles , Bioconjugates , Photochemistry , Photocatalysis , Electrospinning , Polymers , Pollutants , Phenols , Azo dyes
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4293 , http://hdl.handle.net/10962/d1004517 , Phthalocyanines , Gold , Zinc , Nanoparticles , Bioconjugates , Photochemistry , Photocatalysis , Electrospinning , Polymers , Pollutants , Phenols , Azo dyes
- Description: This work presents the syntheses, photophysical and photochemical characterization of arylthio zinc phthalocyanines and their gold nanoparticle conjugates. Spectroscopic and microscopic studies confirmed the formation of the phthalocyanine-gold nanoparticle conjugates which exhibited enhanced photophysicochemical properties in comparison to the phthalocyanines. The studies showed that the presence of gold nanoparticles significantly lowered fluorescence quantum yields and lifetimes. However, this interaction did not restrict the formation of excited singlet and triplet states and hence the formation of singlet oxygen required for photocatalysis. The conjugates showed significantly higher singlet oxygen quantum yields and therefore enhanced photocatalytic activity compared to the phthalocyanines. The zinc phthalocyanines and their gold nanoparticle conjugates were successfully incorporated into electrospun polymer fibers. Spectral characteristics of the functionalized electrospun fibers indicated that the phthalocyanines and phthalocyanine-gold nanoparticle conjugates were bound and their integrity was maintained within the polymeric fiber matrices. The photophysical and photochemical properties of the complexes were equally maintained within the electrospun fibers. The functionalized fibers were applied for the photoconversion of 4-chlorophenol and Orange G as model organic pollutants. , Microsoft� Word 2010 , Adobe Acrobat 9.53 Paper Capture Plug-in
- Full Text:
- Date Issued: 2013
- Authors: Tombe, Sekai Lana
- Date: 2013
- Subjects: Phthalocyanines , Gold , Zinc , Nanoparticles , Bioconjugates , Photochemistry , Photocatalysis , Electrospinning , Polymers , Pollutants , Phenols , Azo dyes
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4293 , http://hdl.handle.net/10962/d1004517 , Phthalocyanines , Gold , Zinc , Nanoparticles , Bioconjugates , Photochemistry , Photocatalysis , Electrospinning , Polymers , Pollutants , Phenols , Azo dyes
- Description: This work presents the syntheses, photophysical and photochemical characterization of arylthio zinc phthalocyanines and their gold nanoparticle conjugates. Spectroscopic and microscopic studies confirmed the formation of the phthalocyanine-gold nanoparticle conjugates which exhibited enhanced photophysicochemical properties in comparison to the phthalocyanines. The studies showed that the presence of gold nanoparticles significantly lowered fluorescence quantum yields and lifetimes. However, this interaction did not restrict the formation of excited singlet and triplet states and hence the formation of singlet oxygen required for photocatalysis. The conjugates showed significantly higher singlet oxygen quantum yields and therefore enhanced photocatalytic activity compared to the phthalocyanines. The zinc phthalocyanines and their gold nanoparticle conjugates were successfully incorporated into electrospun polymer fibers. Spectral characteristics of the functionalized electrospun fibers indicated that the phthalocyanines and phthalocyanine-gold nanoparticle conjugates were bound and their integrity was maintained within the polymeric fiber matrices. The photophysical and photochemical properties of the complexes were equally maintained within the electrospun fibers. The functionalized fibers were applied for the photoconversion of 4-chlorophenol and Orange G as model organic pollutants. , Microsoft� Word 2010 , Adobe Acrobat 9.53 Paper Capture Plug-in
- Full Text:
- Date Issued: 2013
China's African FDI safari : opportunistic exploitation or muturally beneficial to all participants
- Dreier, Tina, Rhodes University
- Authors: Dreier, Tina , Rhodes University
- Date: 2013 , 2013-04-10
- Subjects: Africa -- Foreign economic relations -- China , China -- Foreign economic relations -- Africa , Investments, Foreign -- China , Foreign direct investment
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:929 , http://hdl.handle.net/10962/d1001455 , Africa -- Foreign economic relations -- China , China -- Foreign economic relations -- Africa , Investments, Foreign -- China
- Description: When implemented within a favourable legislative framework, Foreign Direct Investment (FDI) can produce domestic growth-enhancing spillovers in host countries. Other potential positive effects include the provision of investment capital, the creation of local employment and the transfer of sophisticated technology or advanced knowledge. African nations in particular have been historically reliant on externally-provided funds. Prevailing low income levels, marginal savings rates and the absence of functioning financial markets necessary to provide local start-up capital continue to keep Africa reliant on foreign inflows. Considering China’s increasing financial commitments to Sub- Saharan Africa (SSA) over the last decade, this study examines the state of current Sino-African investment relationships. Specific attention is paid to the outcomes of this strategic bilateral alliance in order to determine whether or not a mutually beneficial investment relationship has evolved. The distinct nature and structure of, the motivation behind and the most significant determinants of Chinese FDI to SSA are all analysed in accordance with traditional FDI theories. A case study approach is used to establish whether China’s contemporary interest in SSA differs from historical investments and to also investigate country-specific commonalities and differences. Of particular relevance to SSA are resource-backed Chinese loans that finance major infrastructure projects in host nations. Interestingly, a lot of the Sino-African investment packages resemble similar deals struck between China and Japan in the 1970s. The results of this study indicate that China’s investment motives seem more diverse than initially expected. Resource-seeking, profit-seeking and market access-seeking reasons appear to be the most important motives. After establishing the Top- Ten recipients of Chinese FDI in SSA, these nations are then classified into three major categories: resource-, oil- or agricultural-rich nations. Undiversified resource- or oil-rich economies are found to have secured the largest shares of Chinese FDI. This study suggests that China’s contemporary “African Safari” is an unconventional way of providing financial assistance. Rather than solely supplying FDI, China finances a diverse mix of instruments, the most important being concessional loans, export credits, zero-interest loans and the establishment of Special Economic Zones. A profound difference to traditional Western investment packages is China’s non-interference approach. Accordingly, Beijing not only refrains from intervening in host countries’ domestic affairs but also refuses to attach formal conditionalties to its loans. China’s “financial safari” into Africa has produced many positive as well as negative effects in host countries. Nevertheless, it would seem that the positive effects outweigh the negative and China’s FDI could contribute to sustainable development in SSA
- Full Text:
- Date Issued: 2013
- Authors: Dreier, Tina , Rhodes University
- Date: 2013 , 2013-04-10
- Subjects: Africa -- Foreign economic relations -- China , China -- Foreign economic relations -- Africa , Investments, Foreign -- China , Foreign direct investment
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:929 , http://hdl.handle.net/10962/d1001455 , Africa -- Foreign economic relations -- China , China -- Foreign economic relations -- Africa , Investments, Foreign -- China
- Description: When implemented within a favourable legislative framework, Foreign Direct Investment (FDI) can produce domestic growth-enhancing spillovers in host countries. Other potential positive effects include the provision of investment capital, the creation of local employment and the transfer of sophisticated technology or advanced knowledge. African nations in particular have been historically reliant on externally-provided funds. Prevailing low income levels, marginal savings rates and the absence of functioning financial markets necessary to provide local start-up capital continue to keep Africa reliant on foreign inflows. Considering China’s increasing financial commitments to Sub- Saharan Africa (SSA) over the last decade, this study examines the state of current Sino-African investment relationships. Specific attention is paid to the outcomes of this strategic bilateral alliance in order to determine whether or not a mutually beneficial investment relationship has evolved. The distinct nature and structure of, the motivation behind and the most significant determinants of Chinese FDI to SSA are all analysed in accordance with traditional FDI theories. A case study approach is used to establish whether China’s contemporary interest in SSA differs from historical investments and to also investigate country-specific commonalities and differences. Of particular relevance to SSA are resource-backed Chinese loans that finance major infrastructure projects in host nations. Interestingly, a lot of the Sino-African investment packages resemble similar deals struck between China and Japan in the 1970s. The results of this study indicate that China’s investment motives seem more diverse than initially expected. Resource-seeking, profit-seeking and market access-seeking reasons appear to be the most important motives. After establishing the Top- Ten recipients of Chinese FDI in SSA, these nations are then classified into three major categories: resource-, oil- or agricultural-rich nations. Undiversified resource- or oil-rich economies are found to have secured the largest shares of Chinese FDI. This study suggests that China’s contemporary “African Safari” is an unconventional way of providing financial assistance. Rather than solely supplying FDI, China finances a diverse mix of instruments, the most important being concessional loans, export credits, zero-interest loans and the establishment of Special Economic Zones. A profound difference to traditional Western investment packages is China’s non-interference approach. Accordingly, Beijing not only refrains from intervening in host countries’ domestic affairs but also refuses to attach formal conditionalties to its loans. China’s “financial safari” into Africa has produced many positive as well as negative effects in host countries. Nevertheless, it would seem that the positive effects outweigh the negative and China’s FDI could contribute to sustainable development in SSA
- Full Text:
- Date Issued: 2013
Climate variability and climate change in water resources management of the Zambezi River basin
- Authors: Tirivarombo, Sithabile
- Date: 2013
- Subjects: Water resources development -- Zambezi River Watershed Climatic changes -- Zambezi River Watershed Water-supply -- Zambezi River Watershed Water-supply -- Political aspects -- Africa, Southern Water rights -- Africa, Southern Water security -- Africa, Southern Rain and rainfall -- Africa, Southern Rainfall probabilities -- Africa, Southern Food security -- Africa, Southern Drought forecasting -- Africa, Southern Watersheds -- Africa, Southern Water supply -- Measurement -- Africa, Southern
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:6024 , http://hdl.handle.net/10962/d1002955
- Description: Water is recognised as a key driver for social and economic development in the Zambezi basin. The basin is riparian to eight southern African countries and the transboundary nature of the basin’s water resources can be viewed as an agent of cooperation between the basin countries. It is possible, however, that the same water resource can lead to conflicts between water users. The southern African Water Vision for ‘equitable and sustainable utilisation of water for social, environmental justice and economic benefits for the present and future generations’ calls for an integrated and efficient management of water resources within the basin. Ensuring water and food security in the Zambezi basin is, however, faced with challenges due to high variability in climate and the available water resources. Water resources are under continuous threat from pollution, increased population growth, development and urbanisation as well as global climate change. These factors increase the demand for freshwater resources and have resulted in water being one of the major driving forces for development. The basin is also vulnerable due to lack of adequate financial resources and appropriate water resources infrastructure to enable viable, equitable and sustainable distribution of the water resources. This is in addition to the fact that the basin’s economic mainstay and social well-being are largely dependent on rainfed agriculture. There is also competition among the different water users and this has the potential to generate conflicts, which further hinder the development of water resources in the basin. This thesis has focused on the Zambezi River basin emphasising climate variability and climate change. It is now considered common knowledge that the global climate is changing and that many of the impacts will be felt through water resources. If these predictions are correct then the Zambezi basin is most likely to suffer under such impacts since its economic mainstay is largely determined by the availability of rainfall. It is the belief of this study that in order to ascertain the impacts of climate change, there should be a basis against which this change is evaluated. If we do not know the historical patterns of variability it may be difficult to predict changes in the future climate and in the hydrological resources and it will certainly be difficult to develop appropriate management strategies. Reliable quantitative estimates of water availability are a prerequisite for successful water resource plans. However, such initiatives have been hindered by paucity in data especially in a basin where gauging networks are inadequate and some of them have deteriorated. This is further compounded by shortages in resources, both human and financial, to ensure adequate monitoring. To address the data problems, this study largely relied on global data sets and the CRU TS2.1 rainfall grids were used for a large part of this study. The study starts by assessing the historical variability of rainfall and streamflow in the Zambezi basin and the results are used to inform the prediction of change in the future. Various methods of assessing historical trends were employed and regional drought indices were generated and evaluated against the historical rainfall trends. The study clearly demonstrates that the basin has a high degree of temporal and spatial variability in rainfall and streamflow at inter-annual and multi-decadal scales. The Standardised Precipitation Index, a rainfall based drought index, is used to assess historical drought events in the basin and it is shown that most of the droughts that have occurred were influenced by climatic and hydrological variability. It is concluded, through the evaluation of agricultural maize yields, that the basin’s food security is mostly constrained by the availability of rainfall. Comparing the viability of using a rainfall based index to a soil moisture based index as an agricultural drought indicator, this study concluded that a soil moisture based index is a better indicator since all of the water balance components are considered in the generation of the index. This index presents the actual amount of water available for the plant unlike purely rainfall based indices, that do not account for other components of the water budget that cause water losses. A number of challenges were, however, faced in assessing the variability and historical drought conditions, mainly due to the fact that most parts of the Zambezi basin are ungauged and available data are sparse, short and not continuous (with missing gaps). Hydrological modelling is frequently used to bridge the data gap and to facilitate the quantification of a basin’s hydrology for both gauged and ungauged catchments. The trend has been to use various methods of regionalisation to transfer information from gauged basins, or from basins with adequate physical basin data, to ungauged basins. All this is done to ensure that water resources are accounted for and that the future can be well planned. A number of approaches leading to the evaluation of the basin’s hydrological response to future climate change scenarios are taken. The Pitman rainfall-runoff model has enjoyed wide use as a water resources estimation tool in southern Africa. The model has been calibrated for the Zambezi basin but it should be acknowledged that any hydrological modelling process is characterised by many uncertainties arising from limitations in input data and inherent model structural uncertainty. The calibration process is thus carried out in a manner that embraces some of the uncertainties. Initial ranges of parameter values (maximum and minimum) that incorporate the possible parameter uncertainties are assigned in relation to physical basin properties. These parameter sets are used as input to the uncertainty version of the model to generate behavioural parameter space which is then further modified through manual calibration. The use of parameter ranges initially guided by the basin physical properties generates streamflows that adequately represent the historically observed amounts. This study concludes that the uncertainty framework and the Pitman model perform quite well in the Zambezi basin. Based on assumptions of an intensifying hydrological cycle, climate changes are frequently expected to result in negative impacts on water resources. However, it is important that basin scale assessments are undertaken so that appropriate future management strategies can be developed. To assess the likely changes in the Zambezi basin, the calibrated Pitman model was forced with downscaled and bias corrected GCM data. Three GCMs were used for this study, namely; ECHAM, GFDL and IPSL. The general observation made in this study is that the near future (2046-2065) conditions of the Zambezi basin are expected to remain within the ranges of historically observed variability. The differences between the predictions for the three GCMs are an indication of the uncertainties in the future and it has not been possible to make any firm conclusions about directions of change. It is therefore recommended that future water resources management strategies account for historical patterns of variability, but also for increased uncertainty. Any management strategies that are able to satisfactorily deal with the large variability that is evident from the historical data should be robust enough to account for the near future patterns of water availability predicted by this study. However, the uncertainties in these predictions suggest that improved monitoring systems are required to provide additional data against which future model outputs can be assessed.
- Full Text:
- Date Issued: 2013
- Authors: Tirivarombo, Sithabile
- Date: 2013
- Subjects: Water resources development -- Zambezi River Watershed Climatic changes -- Zambezi River Watershed Water-supply -- Zambezi River Watershed Water-supply -- Political aspects -- Africa, Southern Water rights -- Africa, Southern Water security -- Africa, Southern Rain and rainfall -- Africa, Southern Rainfall probabilities -- Africa, Southern Food security -- Africa, Southern Drought forecasting -- Africa, Southern Watersheds -- Africa, Southern Water supply -- Measurement -- Africa, Southern
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:6024 , http://hdl.handle.net/10962/d1002955
- Description: Water is recognised as a key driver for social and economic development in the Zambezi basin. The basin is riparian to eight southern African countries and the transboundary nature of the basin’s water resources can be viewed as an agent of cooperation between the basin countries. It is possible, however, that the same water resource can lead to conflicts between water users. The southern African Water Vision for ‘equitable and sustainable utilisation of water for social, environmental justice and economic benefits for the present and future generations’ calls for an integrated and efficient management of water resources within the basin. Ensuring water and food security in the Zambezi basin is, however, faced with challenges due to high variability in climate and the available water resources. Water resources are under continuous threat from pollution, increased population growth, development and urbanisation as well as global climate change. These factors increase the demand for freshwater resources and have resulted in water being one of the major driving forces for development. The basin is also vulnerable due to lack of adequate financial resources and appropriate water resources infrastructure to enable viable, equitable and sustainable distribution of the water resources. This is in addition to the fact that the basin’s economic mainstay and social well-being are largely dependent on rainfed agriculture. There is also competition among the different water users and this has the potential to generate conflicts, which further hinder the development of water resources in the basin. This thesis has focused on the Zambezi River basin emphasising climate variability and climate change. It is now considered common knowledge that the global climate is changing and that many of the impacts will be felt through water resources. If these predictions are correct then the Zambezi basin is most likely to suffer under such impacts since its economic mainstay is largely determined by the availability of rainfall. It is the belief of this study that in order to ascertain the impacts of climate change, there should be a basis against which this change is evaluated. If we do not know the historical patterns of variability it may be difficult to predict changes in the future climate and in the hydrological resources and it will certainly be difficult to develop appropriate management strategies. Reliable quantitative estimates of water availability are a prerequisite for successful water resource plans. However, such initiatives have been hindered by paucity in data especially in a basin where gauging networks are inadequate and some of them have deteriorated. This is further compounded by shortages in resources, both human and financial, to ensure adequate monitoring. To address the data problems, this study largely relied on global data sets and the CRU TS2.1 rainfall grids were used for a large part of this study. The study starts by assessing the historical variability of rainfall and streamflow in the Zambezi basin and the results are used to inform the prediction of change in the future. Various methods of assessing historical trends were employed and regional drought indices were generated and evaluated against the historical rainfall trends. The study clearly demonstrates that the basin has a high degree of temporal and spatial variability in rainfall and streamflow at inter-annual and multi-decadal scales. The Standardised Precipitation Index, a rainfall based drought index, is used to assess historical drought events in the basin and it is shown that most of the droughts that have occurred were influenced by climatic and hydrological variability. It is concluded, through the evaluation of agricultural maize yields, that the basin’s food security is mostly constrained by the availability of rainfall. Comparing the viability of using a rainfall based index to a soil moisture based index as an agricultural drought indicator, this study concluded that a soil moisture based index is a better indicator since all of the water balance components are considered in the generation of the index. This index presents the actual amount of water available for the plant unlike purely rainfall based indices, that do not account for other components of the water budget that cause water losses. A number of challenges were, however, faced in assessing the variability and historical drought conditions, mainly due to the fact that most parts of the Zambezi basin are ungauged and available data are sparse, short and not continuous (with missing gaps). Hydrological modelling is frequently used to bridge the data gap and to facilitate the quantification of a basin’s hydrology for both gauged and ungauged catchments. The trend has been to use various methods of regionalisation to transfer information from gauged basins, or from basins with adequate physical basin data, to ungauged basins. All this is done to ensure that water resources are accounted for and that the future can be well planned. A number of approaches leading to the evaluation of the basin’s hydrological response to future climate change scenarios are taken. The Pitman rainfall-runoff model has enjoyed wide use as a water resources estimation tool in southern Africa. The model has been calibrated for the Zambezi basin but it should be acknowledged that any hydrological modelling process is characterised by many uncertainties arising from limitations in input data and inherent model structural uncertainty. The calibration process is thus carried out in a manner that embraces some of the uncertainties. Initial ranges of parameter values (maximum and minimum) that incorporate the possible parameter uncertainties are assigned in relation to physical basin properties. These parameter sets are used as input to the uncertainty version of the model to generate behavioural parameter space which is then further modified through manual calibration. The use of parameter ranges initially guided by the basin physical properties generates streamflows that adequately represent the historically observed amounts. This study concludes that the uncertainty framework and the Pitman model perform quite well in the Zambezi basin. Based on assumptions of an intensifying hydrological cycle, climate changes are frequently expected to result in negative impacts on water resources. However, it is important that basin scale assessments are undertaken so that appropriate future management strategies can be developed. To assess the likely changes in the Zambezi basin, the calibrated Pitman model was forced with downscaled and bias corrected GCM data. Three GCMs were used for this study, namely; ECHAM, GFDL and IPSL. The general observation made in this study is that the near future (2046-2065) conditions of the Zambezi basin are expected to remain within the ranges of historically observed variability. The differences between the predictions for the three GCMs are an indication of the uncertainties in the future and it has not been possible to make any firm conclusions about directions of change. It is therefore recommended that future water resources management strategies account for historical patterns of variability, but also for increased uncertainty. Any management strategies that are able to satisfactorily deal with the large variability that is evident from the historical data should be robust enough to account for the near future patterns of water availability predicted by this study. However, the uncertainties in these predictions suggest that improved monitoring systems are required to provide additional data against which future model outputs can be assessed.
- Full Text:
- Date Issued: 2013
Coming clean: the treatment of traces
- Authors: Wilmot, Cassandra
- Date: 2013 , 2013-09-13
- Subjects: Salcedo, Doris, 1958- Bereavement in art
- Language: English
- Type: Thesis , Masters , MFA
- Identifier: vital:2442 , http://hdl.handle.net/10962/d1006121
- Description: Coming Clean is a mixed media installation which aims to address and express ambivalent feelings or notions of loss arising particularly in the absence of a comprehensive narrative or history by focussing on what remains. By researching the role of indexical signs and strategies, particularly as they are deployed by the artist Doris Salcedo, I explore the capacity for these traces to not only effectively mark and memorialise loss, but to also convey the state of loss as experienced by the bereaved without providing a narrative account of the events which preceded them. The domestic ritual of washing is both alluded to and used as a metaphor in etchings, photographic prints and readymade objects to illustrate my concerns, with particular emphasis on the dialectic between what is revealed or concealed. Using representations or traces of this task of laundry to examine this dialectic between concealment and revelation, I comment on the anxiety of dealing with incomplete narrative inheritances by considering how traces of the past which impose themselves on the present may affect both remembrance and the ritual of loss, while also taking into account the complexities behind the disclosure of sensitive information in families.
- Full Text:
- Date Issued: 2013
- Authors: Wilmot, Cassandra
- Date: 2013 , 2013-09-13
- Subjects: Salcedo, Doris, 1958- Bereavement in art
- Language: English
- Type: Thesis , Masters , MFA
- Identifier: vital:2442 , http://hdl.handle.net/10962/d1006121
- Description: Coming Clean is a mixed media installation which aims to address and express ambivalent feelings or notions of loss arising particularly in the absence of a comprehensive narrative or history by focussing on what remains. By researching the role of indexical signs and strategies, particularly as they are deployed by the artist Doris Salcedo, I explore the capacity for these traces to not only effectively mark and memorialise loss, but to also convey the state of loss as experienced by the bereaved without providing a narrative account of the events which preceded them. The domestic ritual of washing is both alluded to and used as a metaphor in etchings, photographic prints and readymade objects to illustrate my concerns, with particular emphasis on the dialectic between what is revealed or concealed. Using representations or traces of this task of laundry to examine this dialectic between concealment and revelation, I comment on the anxiety of dealing with incomplete narrative inheritances by considering how traces of the past which impose themselves on the present may affect both remembrance and the ritual of loss, while also taking into account the complexities behind the disclosure of sensitive information in families.
- Full Text:
- Date Issued: 2013
Constructions of nationhood in secession debates related to Mthwakazi Liberation Front in Bulawayo's Chronicle and Newsday newspapers in 2011
- Authors: Ndlovu, Mphathisi
- Date: 2013
- Subjects: Newspapers , Bulawayo , Matabeleland , Zimbabwe , Chronicle , Newsday , Secession , Devolution , Nationhood , Ndebele , Ethnic identity , Mthwakazi Liberation Front , Mthwakazi Liberation Front -- Zimbabwe , Mass media and nationalism -- Research -- Zimbabwe , Bulawayo (Zimbabwe) -- Newspapers , Matabeleland (Zimbabwe) -- History -- Autonomy and independence movements , Matabeleland (Zimbabwe) -- Social conditions , Zimbabwe -- Social conditions
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: vital:3415 , http://hdl.handle.net/10962/d1001846
- Description: This study investigates the constructions of nationhood in two Bulawayo newspapers, the Chronicle and Newsday. Against the backdrop of the emergence of a secessionist movement, Mthwakazi Liberation Front (MLF), this research examines the discourses of nationhood in the secessionist debates raging in these two newspapers. This study is premised on a view that nationhood constructions cannot be understood outside the broader context in which these newspapers are embedded. Accordingly, it traces the roots and resurgence of Matabeleland separatist politics, exploring the political-historical forces that have shaped a distinctive Ndebele identity that poses a threat to the one, indivisible Zimbabwean national identity. Further, the study situates Matabeleland separatist politics within the broader African secessionist discourse challenging the post-colonial nation-building project on the continent. Informed by Hall’s (1992, 1996) constructivist approach to identity, it considers national identities as fragmented, multiple and constantly evolving. Thus, this study is framed within Hall’s (1997) constructivist approach to representation, as it examines the constructions of nationhood in and through language. The study uses qualitative research methods, as it examines the meanings of nationhood in key media texts. Informed by Foucault’s discourse theory, this research employs critical discourse analysis (CDA) to analyse 12 articles from the two newspapers. The findings confirm that the representations of nationhood in the two newspapers are influenced by their position within the socio-political context. The state-owned Chronicle legitimates the unitary state discourse advocated by ZANU PF. On the other hand, Newsday’s representations are informed by the discourses of the opposition political parties and civil society that challenge the dominant nation-building project. Thus, within this paper, secession and devolution emerge as alternative imaginaries that contest the authoritarian discourse of nationhood
- Full Text:
- Date Issued: 2013
- Authors: Ndlovu, Mphathisi
- Date: 2013
- Subjects: Newspapers , Bulawayo , Matabeleland , Zimbabwe , Chronicle , Newsday , Secession , Devolution , Nationhood , Ndebele , Ethnic identity , Mthwakazi Liberation Front , Mthwakazi Liberation Front -- Zimbabwe , Mass media and nationalism -- Research -- Zimbabwe , Bulawayo (Zimbabwe) -- Newspapers , Matabeleland (Zimbabwe) -- History -- Autonomy and independence movements , Matabeleland (Zimbabwe) -- Social conditions , Zimbabwe -- Social conditions
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: vital:3415 , http://hdl.handle.net/10962/d1001846
- Description: This study investigates the constructions of nationhood in two Bulawayo newspapers, the Chronicle and Newsday. Against the backdrop of the emergence of a secessionist movement, Mthwakazi Liberation Front (MLF), this research examines the discourses of nationhood in the secessionist debates raging in these two newspapers. This study is premised on a view that nationhood constructions cannot be understood outside the broader context in which these newspapers are embedded. Accordingly, it traces the roots and resurgence of Matabeleland separatist politics, exploring the political-historical forces that have shaped a distinctive Ndebele identity that poses a threat to the one, indivisible Zimbabwean national identity. Further, the study situates Matabeleland separatist politics within the broader African secessionist discourse challenging the post-colonial nation-building project on the continent. Informed by Hall’s (1992, 1996) constructivist approach to identity, it considers national identities as fragmented, multiple and constantly evolving. Thus, this study is framed within Hall’s (1997) constructivist approach to representation, as it examines the constructions of nationhood in and through language. The study uses qualitative research methods, as it examines the meanings of nationhood in key media texts. Informed by Foucault’s discourse theory, this research employs critical discourse analysis (CDA) to analyse 12 articles from the two newspapers. The findings confirm that the representations of nationhood in the two newspapers are influenced by their position within the socio-political context. The state-owned Chronicle legitimates the unitary state discourse advocated by ZANU PF. On the other hand, Newsday’s representations are informed by the discourses of the opposition political parties and civil society that challenge the dominant nation-building project. Thus, within this paper, secession and devolution emerge as alternative imaginaries that contest the authoritarian discourse of nationhood
- Full Text:
- Date Issued: 2013
Contributions of inshore and offshore sources of primary production to the foodweb, and the trophic connectivity between various habitats along a depth-gradient, in Sodwana Bay, Kwazulu-Natal, South Africa
- Authors: Parkinson, Matthew Cameron
- Date: 2013
- Subjects: Marine ecology -- South Africa -- Sodwana Bay , Food chains (Ecology) -- South Africa -- Sodwana Bay , Coastal ecology -- South Africa -- Sodwana Bay , Stable isotopes , Dinoflagellates , Marine algae
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5179 , http://hdl.handle.net/10962/d1001630 , Marine ecology -- South Africa -- Sodwana Bay , Food chains (Ecology) -- South Africa -- Sodwana Bay , Coastal ecology -- South Africa -- Sodwana Bay , Stable isotopes , Dinoflagellates , Marine algae
- Description: Sodwana Bay, situated within the iSimangaliso Wetland Park, is ecologically important as it contains high-latitude corals and the most southerly known population of coelacanths. This thesis utilised stable isotope and lipid analyses to investigate the trophic ecology of the area, in particular, understanding the relative contribution of inshore and offshore primary production to consumers inhabiting intertidal and shallow subtidal, coral reef, deep reef, canyon head and pelagic habitats. Seaweeds, excluding certain species of red seaweeds with highly depleted carbon signatures, and phytoplankton, such as diatoms, were found to be the principal sources of primary production for all consumers. Offshore production was typified by dinoflagellates. Particulate organic matter (POM) was spatio-temporally variable. Three distinct productivity periods related to nutrient cycling were noted with enriched carbon signatures and higher organic matter loads associated with warmer water. Inshore primary production was an important source of carbon to consumers in all habitats with the exception of zooplankton that were more reliant on pelagic primary production. Benthic invertebrates reflected a gradient in the utilisation of inshore production, due to the reduced availability of this source further offshore. Consumers at the furthest sites offshore were found to include a substantial quantity of inshore-derived production in their diets. Fishes, which are more mobile, were found to incorporate a similar proportion of inshore production into their diets regardless of where they were collected from.
- Full Text:
- Date Issued: 2013
- Authors: Parkinson, Matthew Cameron
- Date: 2013
- Subjects: Marine ecology -- South Africa -- Sodwana Bay , Food chains (Ecology) -- South Africa -- Sodwana Bay , Coastal ecology -- South Africa -- Sodwana Bay , Stable isotopes , Dinoflagellates , Marine algae
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5179 , http://hdl.handle.net/10962/d1001630 , Marine ecology -- South Africa -- Sodwana Bay , Food chains (Ecology) -- South Africa -- Sodwana Bay , Coastal ecology -- South Africa -- Sodwana Bay , Stable isotopes , Dinoflagellates , Marine algae
- Description: Sodwana Bay, situated within the iSimangaliso Wetland Park, is ecologically important as it contains high-latitude corals and the most southerly known population of coelacanths. This thesis utilised stable isotope and lipid analyses to investigate the trophic ecology of the area, in particular, understanding the relative contribution of inshore and offshore primary production to consumers inhabiting intertidal and shallow subtidal, coral reef, deep reef, canyon head and pelagic habitats. Seaweeds, excluding certain species of red seaweeds with highly depleted carbon signatures, and phytoplankton, such as diatoms, were found to be the principal sources of primary production for all consumers. Offshore production was typified by dinoflagellates. Particulate organic matter (POM) was spatio-temporally variable. Three distinct productivity periods related to nutrient cycling were noted with enriched carbon signatures and higher organic matter loads associated with warmer water. Inshore primary production was an important source of carbon to consumers in all habitats with the exception of zooplankton that were more reliant on pelagic primary production. Benthic invertebrates reflected a gradient in the utilisation of inshore production, due to the reduced availability of this source further offshore. Consumers at the furthest sites offshore were found to include a substantial quantity of inshore-derived production in their diets. Fishes, which are more mobile, were found to incorporate a similar proportion of inshore production into their diets regardless of where they were collected from.
- Full Text:
- Date Issued: 2013
Coping with HIV and AIDS in marginal communities: a case study of Chivanhu Settlement in Nemanwa, Masvingo, Zimbabwe
- Authors: Makonese, Loveness
- Date: 2013
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3337 , http://hdl.handle.net/10962/d1003744
- Description: This thesis seeks to understand and analyse HIV and AIDS and rural livelihoods in Zimbabwe with particular reference to an isolated and marginalised informal settlement called Chivanhu in Masvingo Province. The focus is specifically on questions around HIV susceptibility, AIDS vulnerability and household resilience. In this regard, it is important to recognise that HIV and AIDS cannot be lumped together as one medical or social condition. Rather, there is a progression from HIV infection to AIDS-related chronic illnesses to possible death, and livelihood strategies often alter along this HIV and AIDS time-line. Zimbabwe for over a decade now has gone through a series of economic and political crises which have impacted detrimentally on both urban and rural livelihoods, even for those households which are not directly affected by the HIV and AIDS pandemic. With the economy in free-fall, households have had to pursue a range of livelihood strategies in order to sustain themselves. These socio-economic conditions have in many ways facilitated susceptibility to HIV infection and vulnerability to AIDS. Many studies have examined this in relation to wellentrenched and stable communities in rural Zimbabwe. But the livelihood dynamics for such communities are significantly different to more unstable and informal settlements like Chivanhu, as thesis seeks to show. At the same time, the thesis offers a longitudinal study which is able to map the changes to the livelihoods of infected and affected households in Chivanhu. Though recognising the debilitating effects of the pandemic on these households, it also raises questions about the possible resilience of certain households despite great adversity. In doing so, it goes beyond the individual and household levels of analysis to consider the role of clusters (or groups of households) in responding to the impacts of HIV and AIDS. In this regard, particular emphasis is placed on gender and orphanhood. In the end, the thesis offers a nuanced analysis of the everyday complexities and challenges for affected households in a marginalised and informal rural community in Zimbabwe and thereby makes a contribution to re-theorising HIV and AIDS and rural livelihoods more broadly.
- Full Text:
- Date Issued: 2013
- Authors: Makonese, Loveness
- Date: 2013
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3337 , http://hdl.handle.net/10962/d1003744
- Description: This thesis seeks to understand and analyse HIV and AIDS and rural livelihoods in Zimbabwe with particular reference to an isolated and marginalised informal settlement called Chivanhu in Masvingo Province. The focus is specifically on questions around HIV susceptibility, AIDS vulnerability and household resilience. In this regard, it is important to recognise that HIV and AIDS cannot be lumped together as one medical or social condition. Rather, there is a progression from HIV infection to AIDS-related chronic illnesses to possible death, and livelihood strategies often alter along this HIV and AIDS time-line. Zimbabwe for over a decade now has gone through a series of economic and political crises which have impacted detrimentally on both urban and rural livelihoods, even for those households which are not directly affected by the HIV and AIDS pandemic. With the economy in free-fall, households have had to pursue a range of livelihood strategies in order to sustain themselves. These socio-economic conditions have in many ways facilitated susceptibility to HIV infection and vulnerability to AIDS. Many studies have examined this in relation to wellentrenched and stable communities in rural Zimbabwe. But the livelihood dynamics for such communities are significantly different to more unstable and informal settlements like Chivanhu, as thesis seeks to show. At the same time, the thesis offers a longitudinal study which is able to map the changes to the livelihoods of infected and affected households in Chivanhu. Though recognising the debilitating effects of the pandemic on these households, it also raises questions about the possible resilience of certain households despite great adversity. In doing so, it goes beyond the individual and household levels of analysis to consider the role of clusters (or groups of households) in responding to the impacts of HIV and AIDS. In this regard, particular emphasis is placed on gender and orphanhood. In the end, the thesis offers a nuanced analysis of the everyday complexities and challenges for affected households in a marginalised and informal rural community in Zimbabwe and thereby makes a contribution to re-theorising HIV and AIDS and rural livelihoods more broadly.
- Full Text:
- Date Issued: 2013
Corporate social responsibility of private game reserves in the Eastern Cape Province
- Authors: Motala, Amina Sadiq
- Date: 2013
- Subjects: Social responsibility of busines -- South Africa -- Eastern Cape Game reserves -- South Africa -- Eastern Cape Ecotourism -- South Africa -- Eastern Cape Environmental responsibility -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1187 , http://hdl.handle.net/10962/d1004335
- Description: The primary aim of this research study was to determine the current state of practice of corporate social responsibility (CSR) within the ecotourism based private game reserve (PGR) industry in the Eastern Cape Province of South Africa. According to Carroll (1991) four kind of responsibilities constitute total CSR, namely economic, legal, ethical and philanthropic responsibilities. Given the context of this research a fifth responsibility concerning environmental responsibilities was added to Carroll 's (1991) pyramid ofCSR. A descriptive and illustrative case study approach, within the phenomenological research paradigm, was used for the purpose of this research. The unit of analysis is the Indalo group, the Association of Eastern Cape Private Game Reserves, made up of 12 ecotourism based PORs. As the current research focused on eight of the twelve PGRs a mUltiple case study was employed. To give effect to the aim a number of research objectives were identified. Firstly to desclibe and assess the current practice in implementing CSR within the ecotourism based PGR industry in the Eastern Cape Province. Secondly, to assess and evaluate the involvement of ecotourism based PGRs in the Eastern Cape Province in each of the five responsibilities comprising CSR. Thirdly, to identify the challenges faced by ecotourism based PGRs in the Eastern Cape Province with regard to the implementation of CSR initiatives. Lastly to provide pertinent conclusions and recommendations with regard to CSR strategies within the ecotourism based PGR industry in the Eastern Cape Province. Data were collected by means of in-depth interviews with the managers of each reserve. In addition the researcher conducted focus interviews with administrative employees at each of the member reserves of the Indalo group. Additionally the managers of each reserve were requested to respond to a structured five point Likert scale questionnaire based on the research by Aupperle, Carroll and Hatfield (1985). This research study found that the current practice at the Indalo group is towards a strategic, long term approach to CSR that aids in the development of a 'sustainable organisation.' The aspect of ' empowerment' emerged as the essence of the current practice ofCSR of the Indalo group. Based on the current practice it was concluded that the Indalo group is actively involved in the specific components of CSR namely, environmental, economic, legal, ethical and philanthropic responsibilities. With regard to the environmental responsibilities it was concluded that the environment is the foundation upon which all other components of CSR rest. In addition it was concluded that the economic responsibilities, if managed well, are key to unlocking the CSR potential of a reserve as well as ensuring widespread economic development. In terms of the legal and ethical responsibilities it was concluded that tourism policies both at the national and international level have not aided in the development of a CSR agenda of the Indalo group. Furthermore managers of the reserves have been instrumental in cultivating their own legal culture of CSR based on their experience in the tourism industry and their ethical stances. In terms of the philanthropic responsibilities it can be concluded that charitable activities that are long term and strategic in nature allow for direct and indirect benefits to all parties involved. Overall, it can further be concluded that despite Hudson and Miller's (2005:5) view that the "tourism industry is well hehind other industries in terms of CSR" the Indalo group is moving forward in terms of rooting CSR into their everyday operations.
- Full Text:
- Date Issued: 2013
- Authors: Motala, Amina Sadiq
- Date: 2013
- Subjects: Social responsibility of busines -- South Africa -- Eastern Cape Game reserves -- South Africa -- Eastern Cape Ecotourism -- South Africa -- Eastern Cape Environmental responsibility -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1187 , http://hdl.handle.net/10962/d1004335
- Description: The primary aim of this research study was to determine the current state of practice of corporate social responsibility (CSR) within the ecotourism based private game reserve (PGR) industry in the Eastern Cape Province of South Africa. According to Carroll (1991) four kind of responsibilities constitute total CSR, namely economic, legal, ethical and philanthropic responsibilities. Given the context of this research a fifth responsibility concerning environmental responsibilities was added to Carroll 's (1991) pyramid ofCSR. A descriptive and illustrative case study approach, within the phenomenological research paradigm, was used for the purpose of this research. The unit of analysis is the Indalo group, the Association of Eastern Cape Private Game Reserves, made up of 12 ecotourism based PORs. As the current research focused on eight of the twelve PGRs a mUltiple case study was employed. To give effect to the aim a number of research objectives were identified. Firstly to desclibe and assess the current practice in implementing CSR within the ecotourism based PGR industry in the Eastern Cape Province. Secondly, to assess and evaluate the involvement of ecotourism based PGRs in the Eastern Cape Province in each of the five responsibilities comprising CSR. Thirdly, to identify the challenges faced by ecotourism based PGRs in the Eastern Cape Province with regard to the implementation of CSR initiatives. Lastly to provide pertinent conclusions and recommendations with regard to CSR strategies within the ecotourism based PGR industry in the Eastern Cape Province. Data were collected by means of in-depth interviews with the managers of each reserve. In addition the researcher conducted focus interviews with administrative employees at each of the member reserves of the Indalo group. Additionally the managers of each reserve were requested to respond to a structured five point Likert scale questionnaire based on the research by Aupperle, Carroll and Hatfield (1985). This research study found that the current practice at the Indalo group is towards a strategic, long term approach to CSR that aids in the development of a 'sustainable organisation.' The aspect of ' empowerment' emerged as the essence of the current practice ofCSR of the Indalo group. Based on the current practice it was concluded that the Indalo group is actively involved in the specific components of CSR namely, environmental, economic, legal, ethical and philanthropic responsibilities. With regard to the environmental responsibilities it was concluded that the environment is the foundation upon which all other components of CSR rest. In addition it was concluded that the economic responsibilities, if managed well, are key to unlocking the CSR potential of a reserve as well as ensuring widespread economic development. In terms of the legal and ethical responsibilities it was concluded that tourism policies both at the national and international level have not aided in the development of a CSR agenda of the Indalo group. Furthermore managers of the reserves have been instrumental in cultivating their own legal culture of CSR based on their experience in the tourism industry and their ethical stances. In terms of the philanthropic responsibilities it can be concluded that charitable activities that are long term and strategic in nature allow for direct and indirect benefits to all parties involved. Overall, it can further be concluded that despite Hudson and Miller's (2005:5) view that the "tourism industry is well hehind other industries in terms of CSR" the Indalo group is moving forward in terms of rooting CSR into their everyday operations.
- Full Text:
- Date Issued: 2013
Counting planes
- Authors: Rawlins, Isabel Bethan
- Date: 2013
- Subjects: Prose poems , Flash fiction , Short stories , English , Creative writing (Higher education) , Short stories, South African (English) -- 21st century , South African fiction (English) -- 21st century , South African poetry (English) -- 21st century , English language -- Writing
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: vital:5966 , http://hdl.handle.net/10962/d1001816
- Description: This collection of prose-poems and flash fiction, together with a few short stories, shows how romantic relationships colour our perspectives on the world. The collection has echoes throughout of speakers' voices, theme, imagery and tone. There is a narrative logic too, but working on a subtle level of echo and resonance
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- Date Issued: 2013
- Authors: Rawlins, Isabel Bethan
- Date: 2013
- Subjects: Prose poems , Flash fiction , Short stories , English , Creative writing (Higher education) , Short stories, South African (English) -- 21st century , South African fiction (English) -- 21st century , South African poetry (English) -- 21st century , English language -- Writing
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: vital:5966 , http://hdl.handle.net/10962/d1001816
- Description: This collection of prose-poems and flash fiction, together with a few short stories, shows how romantic relationships colour our perspectives on the world. The collection has echoes throughout of speakers' voices, theme, imagery and tone. There is a narrative logic too, but working on a subtle level of echo and resonance
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- Date Issued: 2013
Creating a new declaration of rights : a critical reconstruction of earth jurisprudence's global legislative framework
- Authors: Lenferna, Georges Alexandre
- Date: 2013
- Subjects: Universal Declaration , Rights , Earth , Environment , Ethics , Environmental law, International -- Research , Environmental law, International -- Philosophy , Environmental ethics -- Research , Nature conservation -- Law and legislation -- Research , Jurisprudence -- Research , Law -- Philosophy
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: vital:2704 , http://hdl.handle.net/10962/d1001979
- Description: This thesis aims to critique the Universal Declaration of the Rights of Mother Earth and its underlying moral justification in order to provide a stronger and improved version of both. In Chapter 1 I explore what sort of moral justification is necessary to establish the Universal Declaration on firm grounds and explore its relation to environmental ethics and rights discourse. I argue that a non-anthropocentric perspective is necessary to justify the Universal Declaration’s rights. In Chapter 2 I explore the underlying justification of the Universal Declaration as discovered in the works of Cormac Cullinan and Father Thomas Berry. I argue that their ethical framework is indeterminate, has many ambiguities and uncertainties, and, among other problems, it does not provide a clear action-guiding framework. In Chapter 3 I develop an alternative justification for the Universal Declaration. I argue against many predominant moral theories, that in light of our best scientific and moral understanding we should expand the realm of moral concern to include all living beings, a moral theory I call Life’s Imperative. In Chapter 4 I illustrate that Life’s Imperative is a much stronger, more coherent justification for the Universal Declaration, one that coheres with both our best understanding of the natural world and our relation to it, and to an environmental ethic reflective of that relationship. Unfortunately many of the weaknesses in the current implicit justification of the Universal Declaration have also led to it enshrining rights that are themselves problematic. In order to address these issues, I revise its rights to accord with the stronger justification that I established in Chapter 3. The end result of doing so is a revised version of the Universal Declaration
- Full Text:
- Date Issued: 2013
- Authors: Lenferna, Georges Alexandre
- Date: 2013
- Subjects: Universal Declaration , Rights , Earth , Environment , Ethics , Environmental law, International -- Research , Environmental law, International -- Philosophy , Environmental ethics -- Research , Nature conservation -- Law and legislation -- Research , Jurisprudence -- Research , Law -- Philosophy
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: vital:2704 , http://hdl.handle.net/10962/d1001979
- Description: This thesis aims to critique the Universal Declaration of the Rights of Mother Earth and its underlying moral justification in order to provide a stronger and improved version of both. In Chapter 1 I explore what sort of moral justification is necessary to establish the Universal Declaration on firm grounds and explore its relation to environmental ethics and rights discourse. I argue that a non-anthropocentric perspective is necessary to justify the Universal Declaration’s rights. In Chapter 2 I explore the underlying justification of the Universal Declaration as discovered in the works of Cormac Cullinan and Father Thomas Berry. I argue that their ethical framework is indeterminate, has many ambiguities and uncertainties, and, among other problems, it does not provide a clear action-guiding framework. In Chapter 3 I develop an alternative justification for the Universal Declaration. I argue against many predominant moral theories, that in light of our best scientific and moral understanding we should expand the realm of moral concern to include all living beings, a moral theory I call Life’s Imperative. In Chapter 4 I illustrate that Life’s Imperative is a much stronger, more coherent justification for the Universal Declaration, one that coheres with both our best understanding of the natural world and our relation to it, and to an environmental ethic reflective of that relationship. Unfortunately many of the weaknesses in the current implicit justification of the Universal Declaration have also led to it enshrining rights that are themselves problematic. In order to address these issues, I revise its rights to accord with the stronger justification that I established in Chapter 3. The end result of doing so is a revised version of the Universal Declaration
- Full Text:
- Date Issued: 2013