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Ecological assessment of a temperate river system using biomonitoring techniques: a case study of the Bloukrans River system, South Africa

  • Authors: Magadze, Tinotenda
  • Date: 2020
  • Subjects: Bloukrans River , Ecological assessment (Biology) -- South Africa , Stream ecology -- South Africa , Rivers -- Environmental aspects -- South Africa , Benthic animals -- Effect of human beings on -- South Africa , Water quality biological assessment -- South Africa , Diatoms -- South Africa -- Data processing
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/142933 , vital:38177
  • Description: Escalating pressures from growing human populations and environmental impacts increasingly imperil freshwater ecosystems. The Bloukrans River, which drains an urbanised and agricultural catchment in the Eastern Cape province of South Africa, is no exception. Detailed knowledge of the structure and function of the aquatic ecosystems is required in order to create models and matrices that predict, guide assessment and direct intervention on ecological integrity and water quality management in these systems. The main objectives of this research were to: examine the effects of urbanization on the benthic macroinvertebrate functional feeding guild structure among different stream orders; determine if benthic diatoms can be used as effective and reliable indicators of ionic composition and conductivity in different stream order categories and finally; to evaluate the applicability of the South African Diatom Index (SADI) and other indices in the Eastern Cape region of South Africa. Field studies were carried out to explore temporal patterns in community structure (macroinvertebrates and diatoms) and ecosystem function related to land-use patterns, instream habitat availability, and water quality parameters in the Bloukrans River system across four study periods: February (summer) and July (winter) 2016 and February (summer) and May/July (winter) 2018. The study was conducted along a gradient of impacts from less impacted agricultural headwaters to highly impacted urban sites located immediately downstream of the city of Makhanda. Macroinvertebrates were separated into functional feeding groups (FFGs) (i.e. collector-gatherer, collector-filterer, scraper, shredder, and predator) which were then used to assess the effects of selected physico-chemical variables and riparian zone condition on FFG organization. Collector-gatherers were the most abundant in the Bloukrans River and represented 71.3 % of the macroinvertebrate assemblages. Stream order 1species such as Nitzschia palea, Gomphonema parvulum, Tryblionella apiculata, Diploneis vulgaris and Staurosira elliptica. Multivariate analysis (Canonical correspondence analysis (CCA)) indicated that differences in diatom community assemblages were best explained by calcium, magnesium, pH, phosphate, nitrate, dissolved oxygen, sediment nitrate, conductivity and salinity. These results indicate that diatoms can be used as bioindicators for monitoring highly impacted river systems and to also further examine pollution gradients and impacts of specific/point pollution sources. In order to further test the application of diatom indices, nine sites with contrasting water quality were sampled along the length river system in February, May and July 2018. Diatom-based indices incorporated in OMNIDIA software were applied to assess the integrity of the water quality as indicated by diatom communities. For comparative purposes, several foreign indices (e.g. the trophic diatom index (TDI), the percentage pollution-tolerant valves (%PTV), biological diatom index (BDI)) and the South African Diatom Index (SADI) were used in the study. From the results, the Percentage Pollution-Tolerant Valves (%PTV) of most urban sites in the Bloukrans River was above the 20% limit indicating the presence of organic pollutants. Although the foreign diatom indices were applicable in the study, the SADI had significant correlations with most water quality variables (p < 0.05) compared to other indices such as Watanabe Index (WAT), Biological Index of Water Quality Trophic Index (BIWQ) and Trophic Index (TI)). These results support wider use of the SADI as an indicator of water quality conditions in South African river systems. Finally, the observed variations in diatom community structure and composition as a result of changes in water quality were broadly in agreement with the results of macroinvertebrate FFG structure indicating that the two biological indicators can, and should, be used as complementary techniques in the biomonitoring of rivers and streams in South Africa.
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  • Date Issued: 2020

Elucidation of a novel role for HSP70/HSP90 organising protein (Hop) in mRNA processing

  • Authors: Dingle, Laura Margaret Kirkpatrick
  • Date: 2020
  • Language: English
  • Type: thesis , text , Doctoral , Ph.D
  • Identifier: http://hdl.handle.net/10962/59173 , vital:27449 , doi:10.21504/10962/59173
  • Description: Thesis (PhD.)--Rhodes University, Faculty of Science, Biochemistry and Microbiology, 2020.
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  • Date Issued: 2020

Exploring and modelling the effects of agricultural land management and climate change on agroecosystem services in the Eastern Cape, South Africa

  • Authors: Choruma, Dennis Junior
  • Date: 2020
  • Subjects: Agricultural ecology -- South Africa -- Eastern Cape , Agriculture -- Environmental aspects -- South Africa -- Eastern Cape , Crops and climate -- South Africa -- Eastern Cape , Corn -- Climatic factors -- South Africa -- Eastern Cape , Land use -- South Africa -- Eastern Cape
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/146756 , vital:38554
  • Description: The aims of this study were to evaluate the impacts of agricultural land management strategies and climate change on irrigated maize production in the Eastern Cape, South Africa. To achieve these aims, the study was guided by two overarching research questions, subsequently broken down into more specific questions. The first research question examined the reasons behind farmers’ current agricultural land management practices, the values they assigned to different agroecosystem services, their perceptions of climate change and the adaptation strategies they used to address challenges associated with agricultural crop production and climate change. To answer these questions, a survey of conventional farmers in the Eastern Cape was carried out. The survey targeted farmers who used fertilisers and irrigation water in their day to day farming. Results showed that farmers recognised the different benefits that agroecosystems provided even though they were not familiar with the term ‘ecosystem services.’ Farmers assigned a high value to food provisioning compared to other agroecosystem services and managed their farms for maximum crop yields or maximum crop quality. Fertiliser and irrigation water management decisions were based on multiple factors such as cost, availability of farming equipment and crop yield or crop quality considerations. Survey results showed that while most farmers were able to state the amount of fertiliser used per growing season, the majority of farmers did not know the amount of water they used per growing season. From the farmers’ survey it was recommended that extension services and agricultural education programmes be strengthened in the region to increase farmers’ knowledge on effective agricultural land management strategies that support sustainable intensification. The second research question investigated the effects of agricultural land management strategies and climate change on crop yields in the Eastern Cape. This investigation was done in three steps. First, a crop model, the Environmental Policy Integrated Climate (EPIC) model was calibrated and validated using limited field data from maize variety trials carried out at the Cradock Research Farm in the Eastern Cape. Calibration and validation results proved satisfactory with model efficiencies (Nash Sutcliffe, NSE) greater than 0.5 for both calibration and validation. It was concluded that limited data from field trials on maize that only included grain yield and agricultural land management dates can be used for the calibration of the EPIC model to simulate maize production under South African conditions. In the second step, the calibrated model was applied to simulate different irrigation and fertiliser management strategies for maize production in the Eastern Cape. Different irrigation and Nitrogen (N) fertiliser levels were compared to find optimal irrigation and N fertiliser management strategies that would increase maize yields while minimising environmental pollution (nitrate leaching). Model outputs were also compared to the average yields obtained in the field trials (baseline) and to maize yields reported by farmers in the farmers’ survey. Results showed that improved management of irrigation water and N fertiliser could improve farmers’ maize yields from approximately 7.2 t ha-1 to approximately 12.2 t ha-1, an increase of approximately 69%. Results also revealed a trade-off between food provision and nitrate leaching. Simulations showed that increasing N fertiliser application under sufficient irrigation water levels would increase maize yields, however, this would be accompanied by an increase in N leaching. Lastly, the EPIC model was then applied to simulate the effects of future climate change on irrigated maize production in the Eastern Cape. For these simulations, the model was driven by statistically downscaled climate data derived from three General Circulation Models (GCMs) for two future climate periods, (2040-2069) and (2070-2099), under two Representative Concentration Pathways (RCPs): RCP 4.5 and RCP 8.5. Future maize yields were compared to the baseline (1980-2010) maize yield average. All three climate models predicted a decline in maize yields, with yields declining by as much as 23.8% in RCP 8.5, 2070-2099. Simulations also predicted increases in average daily maximum and minimum temperatures for both the two future climate periods under both RCPs. Results also indicated a decrease in seasonal irrigation water requirements. Nitrate leaching was projected to significantly increase towards the end of the century, increasing by as much as 373.8% in RCP 8.5 2070-2099. Concerning farmers’ perceptions of climate change, results showed that farmers were aware of climate change and identified temperature and rainfall changes as the most important changes in climate that they had observed. To adapt to climate change, farmers used a variety of adaptation strategies such as crop rotations and intercropping. Apart from challenges posed by climate change, farmers also faced other challenges such as access to markets and access to financial credit lines, challenges that prevented them from effectively adapting to climate change. The study therefore recommended that appropriate and adequate strategies be designed to help farmers in the region offset the projected decrease in maize production and increase crop yields while minimising negative environmental impacts.
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  • Date Issued: 2020

Exploring teaching and learning German as a foreign language at a South African institution of higher education: blended learning and collocations

  • Authors: Ortner, Gwyndolen Jeanie
  • Date: 2020
  • Subjects: German language -- Study and teaching (Higher) -- South Africa , German language -- Study and teaching (Higher) -- Foreign speakers -- South Africa , German language -- Study and teaching (Higher) -- English speakers -- South Africa , Rhodes University -- Students , Rhodes University. German Studies
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/167295 , vital:41465
  • Description: German Studies students at Rhodes University have normally never studied the German language before enrolling for the first-year course and face the challenge of a fairly rapid linguistic advancement, in a context with very limited exposure to the foreign language outside the classroom. Free writing is an area which students find particularly challenging as it requires students to syndissertatione grammatical and vocabulary knowledge. Furthermore, South African students are often underprepared for the challenges they face at university regarding language, technology and finance, as they try to to assimilate to the academic environment and gain epistemological access to their various subjects, in this case, language acquisition and the attendant modules of translation, literature and cultural studies. The use of technology in teaching and learning, known as blended learning, is said to produce better results than face-to face teaching alone, through creating opportunities for more autonomous student learning. Scholars of instructed second-language acquisition also suggest using technology to improve language instruction. One particular area which is gaining ground is teaching students collocational structures through exploring large language corpora, to improve students’ language competencies such as writing fluency. However, both blended learning practices and corpus-based teaching practices suggested by scholars are often not reported on in a particular teaching and learning context, taking into account factors such as institutional infrastructure, student and staff backgrounds and preparedness, and larger socio-political factors. Thus, it remains unclear how these practices (blended learning and corpus-based teaching of collocations) may be integrated into standard urricula, particularly for languages other than English, which have been severely under-researched. The purpose of this research is to take a context-based approach to language teaching and thereby investigate current blended learning practices for German Studies at Rhodes University in South Africa and explore through an action research approach how to integrate collocational awareness into the curriculum, within the blended learning model. Insights from corpus linguistics guide an adaptation of teaching practice, helping students develop skills to aid with writing fluency, seeking to make the patterned nature of language salient to our students. This is undertaken in a scaffolded way, within the curriculum, making use firstly of the texts to which students are exposed in the textbook (comprehensible input) as a source of collocational examples, and following this by making use of reallife language data from an online German corpus, DWDS. Findings from the study reveal a number of best practices related to the use of blended learning and teaching collocations in context of the German foreign language curriculum at a South African institution of higher education.
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  • Date Issued: 2020

Formulation and characterisation of a combination captopril and hydrochlorothiazide microparticulate dosage form for paediatric use

  • Authors: Chikukwa, Mellisa Tafadzwa Ruramai
  • Date: 2020
  • Subjects: Uncatalogued
  • Language: English
  • Type: thesis , text , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/163216 , vital:41019 , doi:10.21504/10962/163216
  • Description: Thesis (PhD)--Rhodes University, Pharmacy Faculty, Department of Pharmaceutics, 2020
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  • Date Issued: 2020

Formulation development, manufacture and evaluation of a lamivudine-zidovudine nano co-crystal thermo-responsive suspension

  • Authors: Witika, Bwalya Angel
  • Date: 2020
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/140546 , vital:37897 , http://dx.doi.org/10.21504/10962/140546
  • Description: Expected release date-April 2021
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  • Date Issued: 2020

Formulation, development and assessment of devil’s claw loaded phyto-elastosomes in thermo-responsive hydrogels

  • Authors: Ntemi, Pascal Vitalis
  • Date: 2020
  • Subjects: Uncatalogued
  • Language: English
  • Type: thesis , text , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/164568 , vital:41139 , doi:10.21504/10962/164568
  • Description: Thesis (PhD)--Rhodes University, Faculty of Pharmacy, Pharmaceutics, 2020
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  • Date Issued: 2020

History on trial: a study of the Salem commonage land claim

  • Authors: Bezuidenhout, GJW
  • Date: 2020
  • Subjects: South Africa. Restitution of Land Rights Act, 1994 , Salem (South Africa) -- History , Land tenure -- Law and legilstion -- South Africa , Land reform -- Law and legislation -- South Africa
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/146647 , vital:38545
  • Description: This thesis critically examines the Salem commonage claim, a dispute that has shaken the hamlet of Salem to its core. On ground level it has caused racialized fault lines to reopen, while suspicion and distrust has also grown between the black Africans of the area as well. On a national level, the Constitutional Court judgement has potentially set a precedent with regards to its jurisprudential approach in determining the validity of land claims in South Africa. Its interpretation of the law was determined by the restorative justice jurisprudence enshrined in the Restitution of Land Rights Act 22 of 1994 (the Act). It based its own understanding of the history of the commonage on this jurisprudence. In a bold step towards realising the aims and purposes of the Act, the Constitutional Court found that both the black African claimants as well as the white landowners have equal rights to the land. One of the reasons why the decision of the Constitutional Court is ground-breaking is that the dispute involves a former commonage – land used for common purpose. The Constitutional Court emphasised that the Act was an “extraordinary piece of legislation” and had to be interpreted in such a way so as to address the injustices of the past. This included provisions of the Act which dealt with how oral testimonies from claimants would be dealt with. Another interesting feature was the heavy reliance by all parties on expert witnesses in the persons of eminent historians, Professors Martin Legassick and Herman Giliomee. This case gave much-needed clarification as to what the appropriate role of an expert historian witness may be in a land claim. The success or failure of land claims often depend on the weight of the evidence supplied by the expert historian witness. But the historian must also take cognisance of the fact that the evidence s/he gives is appropriate according to the scope of law. This case also dismisses the assumption that colonial instruments of land assignation are beyond reproach. These instruments which grant rights to land may also be scrutinised in a court of law, just like when oral testimony is tested for its credibility. This is important to note, especially when balancing land rights of the claimants against those of the landowners. This thesis agrees with the decision taken by the Constitutional Court in this instance. However, it also cautions that such softly-softly approaches may appear as a suitable compromise on paper, but the feeling on the ground may not be as receptive to reconciliation as what the courts would have hoped for. To the jurist, this judgement accurately encapsulates the purpose and aims of the Act. However, such a judgement may not seem satisfactory to the people of Salem. The decisions of the Salem commonage case are sure to inform the discourse of land claims in South Africa.
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  • Date Issued: 2020

Hop as an anti-cancer drug target

  • Authors: Vaaltyn, Michaelone Chantelle
  • Date: 2020
  • Subjects: Uncatalogued
  • Language: English
  • Type: thesis , text , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/164704 , vital:41156 , doi:10.21504/10962/164704
  • Description: Thesis (PhD)--Rhodes University, Biochemistry and Microbiology, 2020
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  • Date Issued: 2020

How to do things with speeches: a critical discourse analysis of military coup texts in Nigeria

  • Authors: Bello, Umar
  • Date: 2020
  • Subjects: Speeches, addresses, etc., Nigerian -- 20th century -- History and criticism , Critical discourse analysis -- Nigeria , Corpora (Linguistics) , Social sciences -- Philosophy , Intertextuality , Interpellation -- Nigeria , Forensics (Public speaking) , Oratory -- Nigeria , Nigeria -- Politics and government -- 1960- , Nigeria -- Social conditions -- 1960-
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/143336 , vital:38234
  • Description: Coup speeches that usher the military into political power in Nigeria are the central focus of this thesis. There are seven coup speeches that are notable in the changing of the political course in Nigeria and in enabling the military to rule Nigeria for 30 years, establishing another alternative political construct and party (Bangura 1991). The seven coup speeches along with two others, one a colonial proclamation of conquest and the other a counter coup speech (altogether making nine) constitute the data of this thesis. The analysis done here uses Critical Discourse Analysis, based on a combination of Fairclough (1989, 2001), Fairclough and Fairclough (2012), Thompson’s (1984, 1988 and 1990) works with complementary insights by Chilton (2004), to analyze the speeches in order to understand the ideologies, perceptions and arguments of the coup makers enshrined in the texts. I also employ a concordance analytic system in corpus linguistics to sort uses of important terms and lexical items. The analysis is divided into three broad parts, namely: an analysis of representation of social actors and their action, an analysis of the processes of interpellation and then an analysis of the premises of the arguments contained in the speeches. In the concluding part, there is a discussion of the dialectical nature of the coup speeches especially in the areas of mutual influences which aids in the gradual sedimentation of the political ideology of the military. In particular, there is a longitudinal intertextual analysis across all the speeches, from the earliest to the latest, to see how a coup speech genre is created. The contribution of this work to knowledge is in terms of combining discourse analysis and social theory to illuminate some aspects of Nigeria’s socio- political crises in depth and multifariously. This work helps in understanding the nature of Nigerian autocratic democracy, subservient followership by the citizenry and the supremacy of the military elite. The work employs a novel combination of representation, argumentation, interpellation and constitutive intertextuality in understanding military discourse. It looks at speaker intention, the exploitation of interpretation or reception and the formation of subjects in general and each with its importance and social context. The work as a whole reveals that the military try to build legitimacy by way of establishing authority through rhetorical arguments in varying degrees. These arguments are laid bare, and what they discern is that charges are decidedly trumped up by the military against their opponents and constructed to suit the spin of their moments. The coup makers in some instances construct strawmen of opponents and then go ahead to attack their constructed assumptions or they charge without substance using nominalizations, metaphorical constructions and presuppositions. They apply stipulative definitions and emotionally loaded words in evaluating their actions favourably and also in the negative evaluation of the actions of the opponents. At the level of interpellational analysis, the data reveals the use of language in gradually hailing the citizens as military subjects. The role of the audience changes here i.e. from those to be convinced in rhetorical evaluation of opponents to those to be firmly controlled. The persistent hailing and positioning of the citizens as military subjects help in concretizing their subjecthood. The reaction of the people in affirmation of support to the rule of the military is crucial and it completes the interpellation process. As observed by Clark (2007, 141) “many African societies are so inured to military intervention as not to regard it as aberrant”. This inuring of the societies has to do with hegemonic ideological practices in military discourses claiming legitimacy and the right to rule. At the reception level, this shows that most of the citizens have bought into the dominant ideology and are as such interpellated by it or have adopted what Hall (2015, 125) would call the ‘dominant-hegemonic position’. Aspects of argumentation, speech acts, and deontic modals used by the coup makers help in gradually solidifying the subservient nature of the citizens to the military junta. The diachronic and intertextual nature of the analysis also reveals that the colonial proclamation of conquest in Nigeria by Lord Fredrick Lugard possibly influenced the first coup speech in 1966 in terms of structure and genre. There are traces of the colonial proclamations found in the 1966 coup speech. In substance, the military appear to copy their colonial progenitors. Historically, the military were formed as an army of colonial conquest. There is a dialectical interplay between colonial discourse and military coup speeches. The first coup speech, for its part, influences other coup speeches and they in general impact on civilian political language. The work analyzes from the minute to the global and in this bid unties the layers of assumptions, constructions and points of views that underpin an otherwise objective presentation of reality. The study also engages social theory in illuminating aspects of discourse, social practice and political action. The works of post-structuralists like Foucault, Althusser, Bourdieu, Habermas, Laclau and Mouffe, Derrida etc. are employed in shedding light on the processes of social formation in the interpellation of subjects and in the construction of a new political authority by the military regimes.
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  • Date Issued: 2020

Informal learning in local farming practices by rural women in the Lake Chilwa Basin, Malawi: towards coping and adaptation to climate variability and climate change

  • Authors: Mphepo, Gibson Yadunda
  • Date: 2020
  • Subjects: Women -- Non-formal education - Malawi , Non-formal education -- Malawi , Women -- Malawi -- Social conditions , Crops and climate -- Malawi , Agricultural extension work -- Government policy -- Malawi , Environmental education -- Malawi
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/167540 , vital:41490
  • Description: Evidence reveals that informal learning is a neglected research area, globally and nationally. Informal learning, like formal and non-formal learning, is context specific. In the case of my study, the context was in local maize cultivation and the associated local farming practices which are also neglected. Research has shown that rural women in Malawi are significant change agents in socio-economic sectors, yet they are heavily affected by inequality. For example, extreme weather events of droughts and floods in the Lake Chilwa Basin, disproportionately affect more women than men because of their traditional gendered roles such as home care. The complexity of the dualistic nature of being change agents and victims of injustices at the same time offers a catalytic opportunity for potentially transformative social learning for transformative adaptation. Against this backdrop, I conducted a study to investigate and expand informal learning processes to contribute to building the resilience of women and other community members in Domasi and Nsanama Extension Planning Areas (EPA) within the Lake Chilwa Basin. Specifically, the study answered the following question: “How do drought and inter-seasonal dry spells influence informal learning processes to enable transformation adaptation among rural women cultivating maize in the Lake Chilwa Basin, Malawi?” To address the question, the first stage was to review local farming practices and the associated informal learning processes in Malawi. I then used third generation Cultural Historical Activity Theory (CHAT) as an overarching theoretical framework to guide the subsequent research processes which were split into three main phases: mirror data collection for expansive learning, formative change laboratory workshops, and data analysis and reflection. CHAT is a theoretical framework that helps us comprehend and analyze the relationship between the human mind (i.e. what people think and feel) and activity (what people do). It is a formative and activist learning theory that posits learning as occurring through collective activities to meet or change a common object (Mukute and Lotz-Sisitka, 2012, p. 345). For Koszalka and Wu, 2001(p. 493), within a CHAT framework, knowledge is socially constructed by individual learners, building on existing historical experiences, within the learners’ context. To construct this knowledge, learners use technology or mediating tools, as Vygotsky (1978) calls them. To collect mirror data, I conducted focus group discussions, observation studies, and document analysis. I also conducted key informant interviews with selected extension workers responsible for the two case study sites. The hub of my research constituted change laboratory workshops to expand learning through four of the seven expansive learning actions, namely questioning, analysis, modeling and testing the model. One of the essential procedures I relied on to expand learning during these change laboratory workshops was identification and analysis of contradictions that were mirrored back to women. The use of contradictions as fertile ground for learning is premised on Engstrom’s arguments that contradictions form a catalyst for learning. Data were analysed using two approaches: layered and power relations. A layered analysis is a step-by-step process of understanding a situation from the lower to a higher level (mature stage). For my research, this meant understanding sequential learning from questioning (session 1 – lower level) to testing the model (session 8 – higher level). The second data analysis approach, power relations, relates to the Women Empowerment in Agriculture Index (WEAI), a measure of the degree of women empowerment, their agency and inclusion in farming (Ruth et al., 2013, p. 3). I used this type of power analysis tool because my research was agriculture based. Both data analysis approaches relied on N-vivo which is a form of computer-based qualitative data management software. The software was ideal for my study which was also mostly qualitative. During phase 1 of data collection, I identified five local farming practices associated with local maize cultivation, a focus of my study. These practices were slash and burn (mphanje); traditional insect pest control measures; soil fertility enhancement techniques through kuojeka (crop residue incorporation) and livestock manure; traditional weather forecasts; and multiple cropping (mixed and sequential cropping). Among these, the most preferred by the women I interacted with were kuojeka, livestock manure and mixed cropping. I discovered that these local farming practices are informally learned mainly through word of mouth, observation, trials, women-dominated social networks and drama. I also discovered that some of these informal learning pathways are catalyzed by drought and dry spells. For example, during the 1949 and 2002 drought periods, women reported that they had learned new types of coping strategies such as the use of sawdust and banana root flour in place of maize flour to prepare nsima, a staple food in Malawi. During phase 2, change laboratory workshops, I identified 19 contradictions associated with local farming practices, most of which were related to the Government of Malawi bias towards modern farming practices such as hybrids. Other contradictions were related to traditional structures and norms and religion and traditional beliefs. Solutions were suggested for each of the contradictions. Some of these solutions were tested for their workability. These included setting up diversity blocks (demonstration plots) for local maize cultivation under irrigation and engagement of the youth through WhatsApp groups for the first time at the study sites. The results of the tests show that there is potential to transform local farming practices at the study sites and build social resilience against drought and dry spells. For example, from a local maize demonstration plot in Nsanama Extension Planning Area (EPA), farmers learned that kafula local maize is fast maturing and therefore cushions them against hunger as they wait for the main harvest in later months. Eighty-eight households shared local maize seed harvested from the demonstration gardens for upscaling. The Head of Nsanama EPA had also set up another demonstration garden in 2018-2019 growing season consisting of kafula at Nsanama EPA Headquarters for further informal learning purposes. This research has contributed new knowledge to the existing knowledge base about local farming practices and informal learning. These contributions are in the form of methods I used as well as results obtained. Among the key highlights of my contribution to the knowledge base is the development of scenarios as double stimulation tools for the emerging local farming activity system which emanates from the new model solutions resulting from change laboratory workshops. To the best of my knowledge, this was the first time rural communities were engaged in scenario development in Malawi. The first scenarios of this type were developed in 2010 for the Malawi State of Environment and Outlook Report and the process involved middle to senior managers of various institutions in Malawi. Through historical analysis, my research identified local crops that existed in the past but which are currently non-existent or rare. My study also identified unique local farming practices that even puzzled professionals, including the use of ripe banana peels of makumbuka and sukari to eradicate nansongole grass and native bamboos respectively. Both plant species are considered a nuisance in that they colonize land for cultivation. A breakthrough for radical transformation of local farming practices via informal learning requires development and review of relevant policies in Malawi. Such a process requires evidence. This research has provided background information for this process. For those policies already developed, this research has provided information that can help guide implementation of the generalized list of activities outlined in implementation plans of the respective policies.
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  • Date Issued: 2020

Invasion status and impact potential of Florida bass Micropterus floridanus in South Africa

  • Authors: Khosa, Dumisani
  • Date: 2020
  • Subjects: Micropterus floridanus , Florida largemouth bass -- South Africa , Black bass -- South Africa , Micropterus floridanus -- South Africa -- Habitat , Florida largemouth bass -- South Africa -- Habitat , Black bass -- South Africa -- Habitat , Introduced fishes -- Environmental aspects -- South Africa
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/165733 , vital:41276
  • Description: In South Africa, the introduction of alien fishes was largely driven by the development of recreational fishing opportunities. Government-backed programmes resulted in the introduction of four Black Bass species: Micropterus salmoides, Micropterus dolomieu, Micropterus punctulatus and Micropterus floridanus. These species are regarded as the primary threat to native biota and there is an urgent need to assess their spread and impacts. This thesis focuses on: assessing the current distribution of all four Black Basses; using genetic techniques to establish the extent of hybridisation between M. salmoides and M. floridanus, and using functional responses to assess the impact potential of M. salmoides and M. floridanus under the context dependence of temperatures and habitat complexity. Maximum entropy modelling of habitat suitability for Black Bass highlighted that M. salmoides had broad climatic suitability across South Africa, while suitability for M. dolomieu and M. punctulatus was restricted to the Cape Fold Ecoregion. An extensive area-based invasion debt was observed for all Micropterus spp. To better understand the dynamics of hybridisation between M. salmoides and M. floridanus, 38 species-diagnostic single nucleotide polymorphism was used to screen Black Bass populations from the Breede River and Kowie River catchments. Micropterus salmoides alleles were more prevalent than M. floridanus in both the Breede River catchments (69.1% and 30.9%, respectively) and Kowie River catchments (63.3% and 36.7%, respectively). However, isolated populations found in dams (reservoirs) remained free from hybridisation. To compare resource utilisation (i.e. per capita effects) between M. salmoides and M. floridanus, two experimental chapters were designed. Chapter 4 focuses on how temperature mitigates per capita effects between the two Black Basses. There was a significant decrease in per capita effects for M. salmoides with increasing temperatures, while the converse was true for M. floridanus. Chapter 5 gives an account of the influence of habitat complexity on per capita effects. The results showed that habitat complexity did mitigate per capita effects, specifically on M. floridanus, which showed a significant drop in per capita effects; however, no significant difference was recorded for M. salmoides. In the two experimental chapters, M. floridanus exhibited a superior per capita effect compared to M. salmoides, implying higher predation impact on native biota.
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  • Date Issued: 2020

Investigations into biological control options for Lycium ferocissimum Miers, African Boxthorn (Solanaceae) for Australia

  • Authors: Mauda, Evans Vusani
  • Date: 2020
  • Subjects: Lycium ferocissimum , Solanaceae -- Biological control -- Australia , Weeds -- Control -- Australia , Invasive plants -- Biological control -- Australia , Insects as biological pest control agents -- Australia , Insect-plant relationships
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/167142 , vital:41441
  • Description: Lycium ferocissimum Miers (Solanaceae) (African boxthorn or boxthorn) is a shrub native to South Africa,and has become naturalised and invasive in Australia and New Zealand. The plant is listed on the Noxious Weed List for Australian States and territories. Although other control methods are available, biological control presents a potentially sustainable intervention for reducing populations of this weed in Australia. In South Africa, the plant has been recorded from two allopatric populations, one in the Eastern Cape Province, the other in the Western Cape Provinces, however, there taxonomic and morphological uncertainties are reported in the literature. Therefore, before native range surveys for potential biological control agents could be considered, the taxonomic uncertainty needed to be resolved. The two geographically distinct areas, as well as the Australia population were sampled to assess morphological and genetic variation. All samples collected in Australia were confirmed as L.ferocissimum, with no evidence of hybridisation with any other Lycium species. Nuclear and chloroplast genetic diversity within L.ferocissimum across South Africa was high, and Australia was low, with no evidence of genetic seperation. One ehaplotypes found across Australia was found at only two sites in South Africa, both in the Western Cape, suggesting that the Australian lineage may have originated from this region. Ten samples from South Africa, putatively identified in the field as L.ferocissimum, were genetically characterised as different (unidentified) Lycium species. The majority of plants sampled were confirmed as L.ferocissimum, sharing a common haplotype (haplotype 5) with sampled specimens from Australia. Morphological analyses across different Lycium species in South Africa did not identify any leaf or floral characteristics unique to L.ferocissimum, and thus morphological identification in the native range remains problematic. Surveys for phytophagous in sects on L.ferocissimum were carried out regularly over a two-year period in the two regions. The number of insect species found in the Eastern Cape Province (55) was higher than in the Western Cape Province (41), but insect diversity based on Shannon indices was highest in the Western Cape Province. Indicator species analysis revealed eight insect herbivore species driving the differences in the herbivore communities between the two provinces. Based on insect distribution, abundance, feeding preference and available literature, three species were prioritised as potential biological control agents. These include the leaf-chewing beetles, Cassida distinguenda Spaeth (Chrysomelidae) and Cleta eckloni Mulsant (Coccinellidae), and the leaf-mining weevil, Neoplatygaster serietuberculata Gyllenhal (Curculionidae). Native range studies such as this are perhaps the most technically difficult and logistically time-consuming part of the biological control programme. Yet, the entire outcome of a programme depends on the suite of potential agents feeding on the weed. The information gained during this stage significantly contributed to the prioritization of agents for further host-range testing and possible release. Here we showed how molecular and genetic characterisations of the target weed can be us ed to accurately define the identity and phylogeny of the target species. In addition, the study also highlighted the importance of considering plant morphology and how phenotypic plasticity may influence infield plant identifications while conducting native range surveys. By gaining further information during long-term and wide spread native range surveys we were not just able to provide a list of herbivorous insect fauna and fungi associated with the plant, but were able to prioritise the phytophagous species that held the most potential as biological control agents.
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  • Date Issued: 2020

IsiXhosa as the language of teaching and learning mathematics in Grade Six: investigating the mother tongue based bilingual education mathematics pilot in the Eastern Cape Province, South Africa

  • Authors: Mbude, Naledi Ntombizanele
  • Date: 2020
  • Subjects: Mathematics -- Study and teaching (Elementary) -- South Africa , Education, Bilingual -- South Africa , Native language and education -- South Africa , Language policy -- South Africa
  • Language: English
  • Type: text , Thesis , Doctoral , PHD
  • Identifier: http://hdl.handle.net/10962/143262 , vital:38215
  • Description: This study is an investigation on lessons learnt when the language of learners is maintained for teaching and learning mathematics beyond Grade 3 for another 3 years. It is undertaken in Cofimvaba, a rural village of the Eastern Cape in South Africa. We investigate lessons that can be learnt from the Mother Tongue based- Bilingual Education (MTbBE) strategy, that can be replicated. South Africa post-1994 has a Language-in-Education Policy (1997) that provides for the use of all official languages as Languages of Teaching and Learning (LoLT), this has remained on paper as the schooling system focusses on an early –exit model of three years of the Mother Tongue Education (MTE) for the Foundation Phase (FP) then exit to English instruction in Grade 4; this applies to African language learners only. English and Afrikaans speakers have mother tongue education from cradle to university; a benefit they have enjoyed pre- and post-apartheid. Various studies have been conducted to understand the relationship between language and mathematics learning as it is crucial to design mathematics instruction for students who are English Learners (ELs) and/or bilingual. However, in South Africa, there had not been a direct exploration of the achievement of learners in mathematics when their mother tongue is used and sustained throughout the first six years of learning mathematics, while English is a supportive resource. This is the focus of this study. The study lends itself to the adoption of a mixed methods design (QUALT+QUANT), while also employing documents, observation and test scores of learners to obtain data. Content analysis and thematic analysis approaches were used in analyzing the qualitative-type data while a statistical approach was used in the analysis of quantitative data. The main aim of the study was to establish whether in the Cofimvaba pilot, there is any evidence to make a case for extending Mother Tongue Based-bilingual Education (MTBBE) beyond Grade 3 for black African children. Another aim, was to highlight and document the effort that was the first of its kind in South Africa, undertaken in a small rural area to develop isiXhosa as language of Mathematics and Science. The most salient of this effort was the fact that it was underpinned by deliberate theoretical and empirical foundations central to language policy and planning. The finding of this study is that the use of isiXhosa for MTbBE was effective for boosting mathematical and science skills in the mother tongue and English in Grade 6 as demonstrated in Chapter 7 and 8. Lastly, this study demonstrates the power of political will and how a decision backed by financial investment can transform the wider system despite the challenges of transformation. For the first time in the history of education; a poor department has stuck to its guns; unwearied by the negativity surrounding the development of African languages. It committed to the cause of improving the academic achievement of the poorest of the poor. Historically, in implementing a Mathematics Curriculum, the Department of Education (both officials and teachers) has never efficiently implemented the LiEP (1997) in the manner spelt out in its policy documents viz, multilingualism as the norm. The focus has been on a perspective of learners who are learning and must English, then mathematics and ways to get them to know English at all costs. This view creates inequities in the classroom because it places emphasis on what learners don’t know or can’t do. In contrast, this study proposes a sociocultural perspective that shifts away from deficiency models of bilingual learners and instead focuses on describing the resources bilingual students use to communicate mathematically (Moskovich, 1988). Without this shift we will have a limited view of these learners and will design instruction that neglects the competencies they bring to mathematics classrooms. If, instead, we learn to recognize the mathematical ideas these students express in spite of their accents, code-switching, or missing vocabulary, then instruction can build on students’ competencies and resources (Moskovich, 1998). This study recommends a plethora of strategies that must be taken by the Department of Education to widen epistemological access to mathematics for African language learners using MTbBE as a viable strategy.
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  • Date Issued: 2020

Journeying home, exile and transnationalism in Noni Jabavu and Sisonke Msimang’s memoirs

  • Authors: Masola, Athambile
  • Date: 2020
  • Subjects: Home in literature , Exile (Punishment) in literature , Women authors, Black -- South Africa -- History and criticism , Jabavu, Noni -- Criticism and interpretation , Msimang, Sisonke -- Criticism and interpretation
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/166529 , vital:41376
  • Description: This thesis examines the memoirs of two South Africa female writers: Noni Jabavu and Sisonke Msimang. In analysing their memoirs, this thesis sets out to contribute to the scholarship which centres the voices of Black women writers. It explores how both writers interrogate the concepts of home, exile and transnationalism. My focus is on the ways in which the writers theorise questions of belonging, and the extent to which their writing provides the opportunity to give voice to the complex nature of belonging. I read the narratives from these two writers alongside each other, as Noni Jabavu is Sisonke Msimang’s literary ancestor. By looking at their works alongside each other, this thesis becomes an intergenerational conversation between the past and the present. Using Black feminist thought and migratory subjectivity, this thesis examines the ways in which these writers connect the personal and the political. Noni and Sisonke navigate the private spaces of home in conversation with the political experience of exile and the nation. In the second chapter, I explore home as ekhaya, isizwe and an imaginary homeland. Ekhaya is the isiXhosa word denoting the family home, where there is a network of family rather than a nuclear family. Home is also isizwe: the isiXhosa word which evokes the nation state, which takes on layered meanings in the context of colonialism and apartheid. Home can also be an imaginary homeland, which is an imagined version of home after a long absence such as exile. In the third chapter, I examine three forms of exile: internal exile, estrangement and political exile. Finally, in chapter four, I analyse transnationalism: a border-crossing which raises questions of power, mobility and kinship. Looking at home, exile and transnationalism together allows for an exploration which challenges taken-for-granted ideas about place and identity. These memoirs illuminate the ways in which Black women constitute and reconstitute their identities according to the places which they access through travel.
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  • Date Issued: 2020

Long Waves of Strikes in South Africa: 1886–2019

Making South African tertiary education multilingual: the implementation, monitoring and evaluation of SANTED Multilingualism Projects 2007 – 2010

  • Authors: Nosilela, Bulelwa
  • Date: 2020
  • Subjects: South Africa-Norway Tertiary Education Development Programme , Language policy -- South Africa , Multilingual education -- South Africa , Language and education -- South Africa , Education, Higher -- Social aspects -- South Africa
  • Language: English
  • Type: text , Thesis , Doctoral , PHD
  • Identifier: http://hdl.handle.net/10962/140513 , vital:37895
  • Description: This thesis focuses on multilingualism as it applies to education, particularly higher education in South Africa. The thesis suggests that language must be seen as critical in education (Obanya, 2004; Wolff, 2006). This thesis also suggests that there is a link between a person’s general competence in an educational environment, and that person’s language competence. In support of other scholars, this thesis also finds that learners from low social classes in society have a restricted linguistic code and therefore the learners from these social classes lack the ability to express themselves in an elaborative manner in a cognitive situation while learners from upper social class have an elaborate linguistic repertoire in the language of instruction, namely English. Even though general policy in South Africa and Africa as a whole recognises this relationship between language and education, this thesis finds that very little is being done to implement these well-meaning policies. The SANTED (South Africa-Norway-Tertiary-Education) programme, which forms the focus of this thesis, is seen as an exception. While there is an increase in the number of learners and students accessing centres of learning, especially those from historically-disadvantaged backgrounds (CHE Higher Education Monitor, 2008), there is at the same time rigorous debate in South Africa at the moment as to whether these learners and students benefit optimally from knowledge offered in centres of learning, and are therefore, not accomplishing success expected from them (Boughey, 2008). This thesis suggests that at the centre of this debate is the understanding that, while there are a variety of factors that might affect their success, for example social and economic status, schooling background in case of students in higher education, language plays a pivotal role. This thesis analyses the challenges and successes which faced the SANTED programme at a number of South African institutions where language issues were brought to the centre of the learning process. The thesis also assesses best practices in various African countries and makes recommendations regarding language-use in Higher Education, as part of a transformationprocess. The collaboration between the South African and Norwegian governments provided impetus to policy implementation. It also had other focus areas such as capacity building, access, success and retention and collaboration with Southern African Developing Countries (SADC). This thesis shows, through a critical analysis of SANTED, that it represented an important intervention regarding the promotion of multilingualism.
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  • Date Issued: 2020

Mechanism of action of non-synonymous single nucleotide variations associated with α-carbonic anhydrases II, IV and VIII

  • Authors: Sanyanga, T. Allan
  • Date: 2020
  • Subjects: Carbonic anhydrase , Carbonic anhydrase -- Therapeutic use , Nucleotides
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/167346 , vital:41470
  • Description: The carbonic anhydrase (CA) group of enzymes are Zinc (Zn2+) metalloproteins responsible for the reversible hydration of CO2 to bicarbonate (BCT or HCO− 3 ) and protons (H+) for the facilitation of acid-base balance and homeostasis within the body. Across all organisms, a minimum of six CA families exist, including, α (alpha), β (beta), γ (gamma), δ (delta), η (eta) and ζ (zeta). Some organisms can have more than one family, with exception to humans that contain the α family solely. The α-CA family comprises of 16 isoforms (CA-I to CA-XV) including the CA-VIII, CA-X and CA-XI acatalytic isoforms. Of the catalytic isoforms, CA-II and CA-IV possess one of the fastest rates of reaction, and any disturbances to the function of these enzymes results in CA deficiencies and undesirable phenotypes. CA-II deficiencies result in osteopetrosis with renal tubular acidosis and cerebral calcification, whereas CA-IV deficiencies result in retinitis pigmentosa 17 (RP17). Phenotypic effects generally manifest as a result of poor protein folding and function due to the presence of non-synonymous single nucleotide variations (nsSNVs). Even within the acatalytic isoforms such as CA-VIII that llosterically regulates the affinity of inositol triphosphate (IP3) for the IP3 receptor type 1 (ITPR1) and regulates calcium (Ca2+) signalling, the presence of SNVs also causes phenotypes cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CAMRQ3). Currently the majority of research into the CAs is focused on the inhibition of these proteins to achieve therapeutic effects in patients via the control of HCO− production or reabsorption as observed in glaucoma and diuretic medications. Little research has therefore been devoted into the identification of stabilising or activating compound that could rescue protein function in the case of deficiencies. The main aim of this research was to identify and characterise the effects of nsSNVs on the structure and function of CA-II, CA-IV and CA-VIII to set a foundation for rare disease studies into the CA group of proteins. Combined bioinformatics approaches divided into four main objectives were implemented. These included variant identification, sequence analysis and protein characterisation, force field (FF) parameter generation, molecular dynamics (MD) simulation and dynamic residue network analysis (DRN). Six variants for each of the CA-II, CA-IV and CA-VIII proteins with pathogenic annotations were identified from the HUMA and Ensembl databases. These included the pathogenic variants K18E, K18Q, H107Y, P236H, P236R and N252D for CA-II. CA-IV included the pathogenic R69H, R219C and R219S, and benign N86K, N177K and V234I variants. CA-VIII included pathogenic S100A, S100P, G162R and R237Q, and benign S100L and E109D variants. CA-II has been more extensively studied than CA-IV and CA-VIII, therefore residues essential to its function and stability are known. To discover important residues and regions within the CA-IV and CA-VIII proteins sequence and motif analysis was performed across the α-CA family, using CA-II as a reference. Sequence analysis identified multiple conserved residues between the two acatalytic CA-II and CA-IV, and the acatalytic CA-VIII isoforms that were proposed to be essential for protein stability. With exception to the benign N86K CA-IV variant, none of the other pathogenic or benign CA-II, CA-IV and CA-VIII SNVs were located at functionally or structurally important residues. Motif analysis identified 11 conserved and important motifs within the α-CA family. Several of the identified variants were located on these motifs including K18E, K18Q, H107Y and N252D (CA-II); N86K, R219C, R219S and V234I (CA-IV); and E109D, G162R and R237Q (CA-VIII). As there were no x-ray crystal structures of the variant proteins, homology modelling was performed to calculate the protein structures for characterisation. In CA-VIII, the substitution of Ser for Pro at position 100 (variant S100P) resulted in destruction of the β-sheet that the SNV was located on. Little is known about the mechanism of interaction between CA-VIII and ITPR1, and residues involved. SiteMap and CPORT were used to identify binding site amino for CA-VIII and results identified 38 potential residues. Traditional FFs are incapable of performing MD simulations of metalloproteins. The AMBER ff14SB FF was extended and Zn2+ FF parameters calculated to add support for metalloprotein MD simulations. In the protein, Zn2+ was noted to have a charge less than +1. Variant effects on protein structure were then investigated using MD simulations. Root mean square deviation (RMSD) and radius of gyration (Rg) results indicated subtle SNV effects to the variant global structure in CA-II and CA-IV. However, with regards to CA-VIII RMSD analysis highlighted that variant presence was associated with increases to the structural rigidity of the protein. Principal component analysis (PCA) in conjunction with free energy analysis was performed to observe variant effects on protein conformational sampling in 3D space. The binding of BCT to CA-II induced greater protein conformational sampling and was associated with higher free energy. In CA-IV and CA-VIII PCA analysis revealed key differences in the mechanism of action of pathogenic and benign SNVs. In CA-IV, wild-type (WT) and benign variant protein structures clustered into single low energy well hinting at the presence of more stable structures. Pathogenic variants were associated with higher free energy and proteins sampled more conformations without settling into a low energy well. PCA analysis of CA-VIII indicated the opposite to CA-IV. Pathogenic variants were clustered into low energy wells, while the WT and benign variants showed greater conformational sampling. Dynamic cross correlation (DCC) analysis was performed using the MD-TASK suite to determine variant effects on residue movement. CA-II WT protein revealed that BCT and CO2 were associated with anti-correlated and correlated residue movement, highlighting at opposite mechanisms. In CA-IV and CA-VIII variant presence resulted in a change to residue correlation compared to the WT proteins. DRN analysis was performed to investigate SNV effects of residue accessibility and communication. Results demonstrated that SNVs are associated with allosteric effects on the CA protein structures, and effects are located on the stability assisting residues of the aromatic clusters and the active site of the proteins. CA-II studies discovered that Glu117 is the most important residue for communication, and variant presence results in a decrease to the usage of the residue. This effect was greatest in the CA-II H107Y SNV, and suggests that variants could have an effect on Zn2+ dissociation from the active site. Decreases to the usage of Zn2+ coordinating residues were also noted. Where this occurred, compensatory increases to the usage of other primary and secondary coordination residues were observed, that could possibly assist with the maintenance of Zn2+ within the active site. The CA-IV variants R69H and R219C highlighted potentially similar pathogenic mechanisms, whereas N86K and N177K hinted at potentially similar benign mechanisms. Within CA-VIII, variant presence was associated with changes to the accessibility of the N-terminal binding site residues. The benign CA-VIII variants highlighted possible compensatory mechanisms, whereby as one group of N-terminal residues loses accessibility, there was an increase to the accessibility of other binding site residues to possibly balance the effect. Catalytically, the proton shuttle residue His64 in CA-II was found to occupy a novel conformation named the “faux in” that brought the imidazole group even closer to the Zn2+ compared to the “in” conformation. Overall, compared to traditional MD simulations the incorporation of DRN allowed more detailed investigations into the variant mechanisms of action. This highlights the importance of network analysis in the study of the effects of missense mutations on the structure and function of proteins. Investigations of diseases at the molecular level is essential in the identification of disease pathogenesis and assists with the development of specifically tailored and better treatment options especially in the cases of genetically associated rare diseases.
  • Full Text:
  • Date Issued: 2020

Microbial fuel cells for remediation of metal rich wastewater coupled with bioelectricity generation

  • Authors: Mshoperi, Edith
  • Date: 2020
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/124112 , vital:35540
  • Description: Expected release date-April 2022
  • Full Text: false
  • Date Issued: 2020

Multiplexed Mass Spectrometry: Single, On-Bead, Detection Analysis Using MALDI-TOF MS

  • Authors: Twala, Busisiwe Victoria
  • Date: 2020
  • Subjects: Uncatalogued
  • Language: English
  • Type: thesis , text , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/164693 , vital:41155 , doi:10.21504/10962/164693
  • Description: Thesis (PhD)--Rhodes University, Biochemistry and Microbiology, 2020
  • Full Text:
  • Date Issued: 2020
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