https://vital.seals.ac.za/vital/access/manager/Index ${session.getAttribute("locale")} 5 https://vital.seals.ac.za/vital/access/manager/Repository/vital:70167 Wed 31 Jan 2024 18:01:05 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70158 Wed 29 Nov 2023 19:04:58 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:18481 Wed 28 Feb 2024 12:41:52 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:41450 Wed 28 Feb 2024 12:37:06 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:41456 Wed 28 Feb 2024 12:31:46 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:40805 0.01). Ninety three percent of S. aureus that were confirmed as MRSA with PCR method possessed mecA gene, whilst 16% of S. epidermidis had mecA and 33% possessed icaA gene. Fifty eight percent (58%) of S. aureus possessed luks-lukf PVL gene suggesting that it may have been acquired from the community. Forty two percent 42% of msrA was detected in S. epidermidis, 43% ermC and 34% ermA were detected in S. aureus isolates. Eleven percent 11% of S. aureus isolates were found to carry both ermA and ermC genes. mecA and luks-lufPV genes are implicated in iv methicillin resistant S. aureus that causes necrotizing pnemoniae and icaA is implicated in the formation of biofilm, another mechanism of resistance in S. epidermidis. RAPD profile resulting in DNA amplification fragments ranged in size from 300 to 1500 bp. The RAPD patterns obtained showed various trends among isolates meaning that S. aureus and S. epidermidis isolates investigated had high diversity among them. Dendograms constructed with each oligonucleotide showed that PCR patterns obtained with primer OPL11 (S. aureus) and ERIC1 (S. epidermidis) strains had genetic similarity indices ranging from 0.009 to 0.329. This wide range of similarity indices indicated a high level of DNA polymorphism among S. aureus and S. epidermidis isolates. Knowledge of the genes coding for resistance in this study will address the gaps in the mechanisms of resistance for effective intervention strategies. The infections caused by multi-resistant strains of Staphylococcal species represent an important problem that affects many health institutions. Due to the large number of patients infected with S. aureus and S. epidermidis and the diverse possibilities of contamination, it is always necessary to review the evolution of antimicrobial resistance and the therapeutic responses of the bacterial strains. This aspect of the study provides updated data on susceptibility patterns of local isolates of S. aureus and S. epidermidis for use in empiric management of patients including the various genes coding for resistance in the designated pathogens and their phylogenetic relatedness. Chapter 3 presents the antimicrobial and anti-HIV1 activities of selected medicinal plants from the Eastern Cape Province used for treating skin and respiratory tract infections. Anti-HIV activities were investigated because of the role of staphylococcal v species as opportunistic pathogens in HIV infections. Four medicinal plants were evaluated for antimicrobial activities and cytotoxicity on clinical isolates of S. aureus and S. epidermidis. Medicinal plants used in this study were Cassine transvaalensis, Croton gratissimus, Vangueria infausta and Vitex ferruginea. The Kirby-Bauer disk diffusion and 96 well microtiter plate methods were used in the antimicrobial activity studies but for the anti-HIV1 activity, MAGI assay was used. Microtetrazolium assay (MTT) was used for the evaluation of cytotoxicity of plants. Bio-autographic assay was used to locate the position of the active compound using Rf value; thin layer chromatography and column chromatography were used in the isolation of active compounds and NMR was used in the identification and structural elucidation of the active compounds. C. transvaalensis and V. infausta were found to possess high activity against S. aureus and S. epidermidis with the lowest MIC of 0.02. In the anti HIV1 MAGI assay, C. transvaalensis and C. gratissimus showed activity against HIV1. C. transvaalensis and C. grattisimus possessed high level of toxicity with CC50 reading of 0.2 while V. infausta and V. ferruginea had reading of 0.1. Two triterpenoids were successfully isolated in C. transvaalensis. Elucidation of the structure of active compounds of plant extracts of medicinal plants provides suitable templates for candidate drug designs and drug discovery. Such potential application will be contingent on the results of cytotoxicity testing and this underlines the significance of the test. Human serum is an important host defence mechanism against disease causing agents. Chapter four evaluates the bactericidal activity of different blood groups. Blood samples of different blood groups were screened for the presence of antibodies against S. aureus and S. epidermidis using agglutination test. Serum sensitivity assay vi was employed for susceptibility testing of S. aureus and S. epidermidis to blood samples. The Gunea-pig serum was used to determine the role of complement in serum bactericidal activity. The results showed that S. aureus and S. epidermidis were highly sensitive to normal human sera from blood group B with a percentage of 61% (S. aureus) and 83.3% (S. epidermidis). When the role of complement in bactericidal activity of the serum to staphylococci species was assessed it was shown that when both S. aureus and S. epidermidis were incubated with individual sera of blood groups and pooled serum of the same blood groups, the organisms were serum sensitive. The findings showed that different individual and pooled serum samples had a role to play in the defence mechanisms of individuals against S. aureus and S. epidermidis. In general the study provides a reference document on S. aureus and S. epidermidis in terms of their antibiograms, molecular characterization, sensitivity to human sera and susceptibility to medicinal plant extracts. Cytotoxicity profiles of the medicinal plants are also reported. Due to the on-going need to expand on the frontiers of knowledge, it is therefore recommended, as a way of building on this study, extensive investigations on the epidemiology of S. epidermidis, S. aureus and related pathogens to be conducted because studies of this nature have been limited by the fact that S. epidermidis strains are often considered to be contaminants. High-level surveillance of clinical samples of MRSA to define the instance and spread of CA-MRSA and HA-MRSA in hospital settings including health care workers and patients are also warranted in addition to further anti -cancer activities and cytotoxicity tests.]]> Wed 28 Feb 2024 12:26:09 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:45324 Wed 27 Sep 2023 08:55:07 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69943 Wed 22 Nov 2023 13:01:41 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69765 Wed 15 Nov 2023 11:34:54 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69762 Wed 15 Nov 2023 10:45:02 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:41619 80 μg∙L-1, ≥ 2781 H. akashiwo cells∙mL-1). Dissolved oxygen differed significantly by bloom state, with the highest dissolved oxygen concentrations. Larval fish density, diversity and community structure variability were lower during hypereutrophic blooms compared to standard blooms and bloom absence, albeit statistically insignificant. Estuarine resident larval density was negatively correlated with dissolved oxygen concentration (P < 0.001). Dominant prey copepod of G. aestuaria larvae, P. hessei, was less abundant and variable during hypereutrophic blooms (mean: 664, range: 4-4301 individuals per m3) compared to standard blooms (mean: 950, range: 2-5237 individuals per m3) and bloom absence (mean: 1182, range: 23-7259 individuals per m3). Although HABs did not appear to drive a mismatch between prey and G. aestuaria larvae, early juvenile larvae made only a small contribution to the assemblage (0.2-1.8%). In terms of predator effects, yolk sac larvae were negatively related to R. terranatalis (P < 0.05). A significant positive relationship was found between prey, P. hessei and G. aestuaria preflexion (P < 0.001), flexion (P < 0.001) and postflexion (P < 0.05) larvae. In a world first, the effects of HABs were investigated on a larval fish species, Gilchristella aestuaria, using biochemical body condition analyses. During severe bloom conditions, G. aestuaria larvae were smaller and the assemblage consisted of mainly early developmental stages (stats). Body condition (RNA per dry weight) was poorest in the presence of hypereutrophic blooms (P < 0.001). A bell-curved relationship between RNA/DW and dissolved oxygen concentration was also produced in a 3-day time-lagged GAM (P < 0.001). In conclusion, hypereutrophic bloom conditions appeared to trigger adult G. aestuaria spawning in the upper mesohaline zone of the estuary in response to heightened primary productivity which occurs during the early bloom stage. However, reductions in copepod abundance (P. hessei) and unfavourable oxygen concentrations interfered with grow-out of G. aestuaria larvae. This research showed that HABs can alter nursery properties to the detriment of G. aestuaria larvae can have serious knock-on effects up the food chain. The loss in abundance of adult, juvenile and larval G. aestuaria, a key forage fish in South African estuaries, has major implications for estuarine food webs, especially in reduced prey availability to juvenile fishes and birds. There is still much to learn about the effects of HABs on the physiological and behavioural aspects on plankton communities worldwide.]]> Wed 13 Mar 2024 14:10:51 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:35643 Wed 13 Mar 2024 11:50:28 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:31855 Wed 13 Mar 2024 11:29:38 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:31348 Wed 13 Mar 2024 11:29:23 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:38920 Wed 13 Mar 2024 11:22:14 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:62120 Wed 13 Mar 2024 11:14:22 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:36716 Wed 13 Mar 2024 11:08:45 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:29807 Wed 13 Mar 2024 10:05:29 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:33886 Wed 13 Mar 2024 10:05:10 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27123 Wed 13 Mar 2024 09:58:13 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27069 Wed 13 Mar 2024 09:44:50 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:41606 Wed 13 Mar 2024 09:37:38 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28550 Wed 13 Mar 2024 09:02:27 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70634 Wed 13 Dec 2023 09:32:10 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71972 Wed 10 Apr 2024 14:10:40 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69559 Wed 08 Nov 2023 12:23:05 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69556 Wed 08 Nov 2023 11:49:43 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69397 Wed 01 Nov 2023 10:44:14 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69393 Wed 01 Nov 2023 09:30:22 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71404 Tue 30 Jan 2024 10:35:07 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:41894 Tue 27 Feb 2024 15:59:05 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:41899 Tue 27 Feb 2024 15:58:21 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:37776 Tue 27 Feb 2024 09:02:00 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:41891 Tue 27 Feb 2024 08:38:58 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28277 Tue 12 Mar 2024 15:33:46 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:33896 Tue 12 Mar 2024 15:27:11 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:36614 5m). This study aimed to address this information gap. Dive surveys were conducted along 10 m long transects (~3 reps) at three depth zones (<1m; 1-2m; > 2m) for four sites along the span of the ranching concession area. Similar assessments were done at a seeded site at the Noordhoek Ski Boat Club and an unseeded site at the Willows area, in order to reveal whether seeding had any impact on the benthic community. Images from a GoPro mounted in the centre of a framer unit (0.5 m2) were taken every 0.5 m along the transect. Macroalgal and macrofaunal cover was determined from these images, and the benthic community characterised from these data. Seaweed samples were taken for species identification. In the four baseline sites, sampling was done at three depth zones to note any changes with a depth gradient, both in terms of substrate type, as well as community composition. There was a notable trend with substrate type having a significant influence (P<0.05) on the community structure. Seaweed communities were dominated by Plocamium corallorhiza and coralline turf based on substrate types. There was also a significant relationship (P<0.05) between substrate type, dominant seaweed species and abalone presence. A Canonical Correspondence Analysis (CCA) of the community data suggested that the benthic community does not change significantly along the distance of coastline sampled. A hierarchical cluster analysis of Bray-Curtis dissimilarity for transect data also suggested that the four sites do not represent ecologically dissimilar communities. A similar analysis showed that abalone seeding had to date not altered the benthic community in shallow seeded areas from the community described for shallow unseeded areas. The study was used to describe the baseline benthic community in areas west of Cape Recife, examine the natural variability along the coast, and determine whether there are relationships between the benthic community composition and emergent abalone abundance. This information will be useful in selection of habitats for abalone seeding in the future. It is important that monitoring and surveying of the study area are to be continued in so allowing for long term data collection which will help in making informed decisions as well as documenting the impact in seeded areas when compared to unseeded areas.]]> Tue 12 Mar 2024 15:12:54 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70618 Tue 12 Dec 2023 13:39:22 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71829 Tue 09 Apr 2024 14:30:48 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70286 Tue 09 Apr 2024 14:30:47 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71866 Tue 09 Apr 2024 14:30:46 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:68650 Tue 09 Apr 2024 08:54:43 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69494 Tue 07 Nov 2023 10:15:41 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:38302 Tue 06 Feb 2024 11:07:54 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71346 Thu 25 Jan 2024 13:24:48 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71341 Thu 25 Jan 2024 13:11:46 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:29474 Thu 18 Jan 2024 09:05:18 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:35412 Thu 18 Jan 2024 09:01:09 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:30652 Thu 18 Jan 2024 08:53:27 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:38884 Thu 18 Jan 2024 08:51:21 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69807 Thu 16 Nov 2023 15:07:51 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69792 Thu 16 Nov 2023 11:42:23 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69789 Thu 16 Nov 2023 09:36:59 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65775 100 kDa through ultracentrifugation. Mass spectrometry also detected the most abundant peak for all fucoidans to be around 700 Da (m/z). Extracted fucoidans inhibited the activity of α-glucosidase more strongly than the commercial anti-diabetic agent acarbose but were inactive on α-amylase. Fucoidans were also shown to be mixed inhibitors of α-glucosidase. Compellingly, fucoidans synergistically inhibited α-glucosidase in combination with the anti-diabetic agent acarbose, highlighting prospects for combination therapy. Finally, fucoidans demonstrated some anti-proliferative characteristics on HCT116 cancer cells by inhibiting their ability to adhere to the tissue culture plate matrix. Furthermore, some fucoidan extracts inhibited the migration of HCT116 cancer cells from 3D spheroids. Some of our fucoidan extracts also inhibited HCT116 colony formation, demonstrating inhibition of long-term cell survival. The E. maxima water extract also inhibited glucose uptake by HCT116 cells, thereby influencing the glycolytic flux. In conclusion, biologically active fucoidans were successfully extracted from South African brown seaweeds. These fucoidans demonstrated anti-diabetic and anti-cancer properties, revealing their relevance as potential drugs for these diseases.]]> Thu 11 Jan 2024 18:08:05 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65763 200 km). These characteristics provide opportunities for high levels of connectivity, and as such, the stock structure is not well understood. We applied an integrated, multidisciplinary approach to provide an improved understanding of the complex stock structure dynamics for Patagonian toothfish on the Patagonian Shelf, specifically in relation to the shelf, slope, and deep-sea plains around the Falkland Islands. Research results were focused on aspects pertaining to (1) geographic variation in phenotypic characters (otolith shape); (2) a description of the spatial-temporal distribution patterns; (3) the active movements of deep-sea adults (tag-recapture study); and (4) the identification of early life-history dispersal through otolith microstructure and microchemical chronologies. Results from the study indicate high regional connectivity during the early life-history stages derived from at least two spawning contingents into spatially discrete nursery areas (cohort groups) on the Falklands Shelf. Fish followed distinct ontogenetic pathways into deeper waters adjacent to the areas wherein juvenile settlement into a demersal habitat occurred. There is little to no evidence of mixing among cohort groups during their ontogenetic migration into deep-sea adult habitats, reflecting a mixed population based on oceanographically defined egg and larval dispersal. The majority of the adult component of the population continue to display high site fidelity. However, between 9 and 25% of the population, consisting predominantly of larger reproductively capable adults undertake long-distance dispersal behaviour, identified as home-range relocations from the adult deep-sea habitats towards three of the known southern spawning grounds in the region. Results are suggestive of a requirement for improved collaborative efforts for regionally-based management approaches with careful consideration of local stock contingents. Future monitoring and research priorities should focus on the identification of reproductive potential, dispersal pathways and settlement patterns of stock contingents to inform the dynamics of mixed stock origins across the Patagonian region. While many aspects regarding the stock structure remain unresolved, results derived from the current studies can be used to inform the development of management measures to ensure the continued recovery and sustainable management of Patagonian toothfish within the region.]]> Thu 11 Jan 2024 18:07:47 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:53108 Thu 07 Mar 2024 15:14:58 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:49059 Thu 07 Mar 2024 14:36:59 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:68642 Thu 05 Oct 2023 12:22:46 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:68641 Thu 05 Oct 2023 12:15:18 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70175 Thu 04 Jan 2024 11:00:40 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69909 Thu 04 Jan 2024 10:53:28 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71938 Thu 04 Apr 2024 19:37:42 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71930 Thu 04 Apr 2024 18:57:36 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71928 Thu 04 Apr 2024 17:34:04 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71925 Thu 04 Apr 2024 16:51:48 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71625 Sun 25 Feb 2024 13:35:22 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65380 Sun 21 Jan 2024 14:30:44 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65792 Sun 21 Jan 2024 14:23:44 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69919 Sun 21 Jan 2024 14:16:49 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:38763 Sun 03 Dec 2023 15:14:11 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71655 Sat 24 Feb 2024 19:10:47 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70157 Sat 23 Dec 2023 17:40:05 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:19965 Sat 09 Sep 2023 09:32:33 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70532 Sat 09 Dec 2023 20:29:18 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71963 Sat 06 Apr 2024 14:59:11 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71961 Sat 06 Apr 2024 14:42:26 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71957 Sat 06 Apr 2024 13:33:28 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71956 4 and Dst_ -50 nT was used as a criterion for geomagnetic disturbed conditions, while the four geomagnetically quiet days were selected each month based on Kp < 3. Observations of TIDs are made using Global Navigational Satellite Systems (GNSS) total electron content derived data. During quiet conditions, seven and two transhemispheric TIDs were identified over the African-European and American sectors, respectively. The observed TIDs originated from the wintertime hemisphere and propagated into the summertime hemisphere. The horizontal velocity, periods, and horizontal wavelengths of TIDs are in range of cH = 120-274 m/s, 48-80 min and _H = 379-1104 km, respectively. These quiet-time equatorward TIDs have been associated with tertiary gravity waves (GWs) from the dissipation of secondary GWs which are in turn generated from the dissipation of mountain waves (MWs) as a result of excited orographic forcing. The poleward TIDs during geomagnetically quiet conditions over the African and American sectors occur mainly during local daytime. Poleward TIDs were observed mostly in the African-European sector than the American sector. Their horizontal propagation velocities and periods range between 129-280 m/s and 39-70 min over African-European and American sectors. Although the mechanisms responsible for launching quiet-time poleward TIDs have not been established in this study, lower atmospheric processes such as convection systems, sudden stratospheric warming and cold weather fronts may have a role in their generation. During geomagnetic storms in the African sector, almost all poleward TIDs (with the exception of two cases) during the main phase were large-scale with horizontal velocities and periods ranging from 250-503 m/s and 30 min to 2 hours. During recovery phase, poleward TIDs fall under the category of medium scale. In the American sector, the majority of poleward TIDs occurred during the storm's main phase, as opposed to the African-European sector, which experienced a significant number of poleward TIDs during the recovery phase. The periods and horizontal velocities of TIDs range from 45 min-1.5 h and 180-296 m/s during main phase. During the recovery phase, the horizontal velocity and period range from 177-271 m/s and 40-1.5 h, respectively. Overall, it has been shown that statistically, changes in equatorial electrodynamics related to enhanced eastward electric _eld and hence increased equatorial electrojet (vertical E_B drift) correlates highly with the reported poleward TIDs.]]> Sat 06 Apr 2024 13:06:52 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71954 Sat 06 Apr 2024 12:24:44 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71953 Sat 06 Apr 2024 12:11:21 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:61170 Mon 08 Jan 2024 12:35:27 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:67337 Mon 04 Sep 2023 11:42:49 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:41905 Mon 04 Mar 2024 14:28:26 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:46450 Mon 04 Mar 2024 14:24:33 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:30607 Mon 04 Mar 2024 14:17:38 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5314 Mon 04 Dec 2023 09:28:51 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:66371 Mon 02 Oct 2023 08:26:57 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70169 Fri 19 Apr 2024 08:27:46 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69847 Fri 17 Nov 2023 14:53:15 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69844 Fri 17 Nov 2023 14:19:01 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70512 Fri 16 Feb 2024 17:32:59 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:39587 Fri 15 Mar 2024 13:12:42 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:36669 Fri 15 Mar 2024 13:06:48 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:30949 Fri 15 Mar 2024 13:00:57 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:31850 Fri 15 Mar 2024 13:00:43 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69851 Fri 15 Mar 2024 12:52:01 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:8354 Fri 15 Mar 2024 12:06:17 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70688 Fri 15 Mar 2024 12:04:38 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:59331 Fri 01 Mar 2024 14:44:27 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:43105 Fri 01 Mar 2024 11:51:51 SAST ]]>