- Title
- Screening of shark liver extracts for potential antimicrobial properties against selected pathogenic bacterial strains
- Creator
- Mrwetyana, Thandolwethu
- Subject
- Fishes -- Diseases Pathogenic bacteria
- Date Issued
- 2017
- Date
- 2017
- Type
- Thesis
- Type
- Masters
- Type
- Microbiology
- Identifier
- http://hdl.handle.net/10353/13004
- Identifier
- vital:39431
- Description
- The growing problem of antimicrobial resistance prevents the effective treatment of bacterial infections. Traditional antibiotics such as penicillin have been rendered ineffective against most microbial pathogens. This has led to an increased need for the development of new and improved drugs. The marine environment contains a great array of organisms with unique biological properties, but still remains one of our most underutilized biological resources. The aim of this study was to screen different shark liver extracts for antimicrobial properties. After optimizing the extraction methods, the liver extracts (oil and aminosterol) of three different shark species, namely the Dogfish (Squalus acanthias), the Catshark (Scyliorhinus capensis) and the Hammerhead (Sphyrna zygaena) shark, were screened for antimicrobial properties using the Kirby-Bauer disc diffusion method against selected bacterial pathogens (Helicobacter pylori, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus cereus), after which the MIC was determined using the modified broth micro-dilution described by Vollekova et al. (2001). The most active extract was fractionated using thin layer chromatography, and TLC-direct bioautography was used to determine the antimicrobial properties of the fractionated compounds. The Folch et al and the Shinnar et al methods yielded the highest extract volumes for oil and aminosterol consecutively, and the catshark and dogfish aminosterol extract showed greater levels of bioactivity against all selected bacterial pathogens, with S. aureus showing highest susceptibility levels to both extracts. A total of 22 compounds were observed in the developed plates with two compounds (Rf 0.53 and 0.79) showing antimicrobial activity. Certain shark liver extracts possess antimicrobial properties that have the potential to be used in the development of new antimicrobial drugs.
- Format
- 98 leaves
- Format
- Publisher
- University of Fort Hare
- Publisher
- Faculty of Science and Agriculture
- Language
- English
- Rights
- University of Fort Hare
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UFH Department of Biochemistry & Microbiology
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