- Title
- Real-time monitoring of 3T3-L1 preadipocyte differentiation using a commercially available electric cell-substrate impedance sensor system:
- Creator
- Kramer, Adam H
- Creator
- Joos-Vandewalle, Julia
- Creator
- Edkins, Adrienne L
- Creator
- Frost, Carminita L
- Creator
- Prinsloo, Earl
- Date Issued
- 2014
- Date
- 2014
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/164830
- Identifier
- vital:41176
- Identifier
- DOI: 10.1016/j.bbrc.2013.12.123
- Description
- Real-time analysis offers multiple benefits over traditional end point assays. Here, we present a method of monitoring the optimisation of the growth and differentiation of murine 3T3-L1 preadipocytes to adipocytes using the commercially available ACEA xCELLigence Real-Time Cell Analyser Single Plate (RTCA SP) system. Our findings indicate that the ACEA xCELLigence RTCA SP can reproducibly monitor the primary morphological changes in pre- and post-confluent 3T3-L1 fibroblasts induced to differentiate using insulin, dexamethasone, 3-isobutyl-1-methylxanthine and rosiglitazone; and may be a viable primary method of screening compounds for adipogenic factors.
- Format
- 6 pages
- Format
- Language
- English
- Relation
- Biochemical and biophysical research communications
- Relation
- Kramer, A.H., Joos-Vandewalle, J., Edkins, A.L., Frost, C.L. and Prinsloo, E., 2014. Real-time monitoring of 3T3-L1 preadipocyte differentiation using a commercially available electric cell-substrate impedance sensor system. Biochemical and biophysical research communications, 443(4), pp.1245-1250
- Relation
- Biochemical and biophysical research communications volume 443 number 4 1245 1250 January 2014 1090-2104
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Elsevier Terms and Conditions Statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)
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Prinsloo, Earl (Dr)
RU DST/NRF SARChI for Molecular and Cellular Biology of the Eukaryotic Stress Response
Edkins, Adrienne Lesley (Associate Prof)
RU Department of Biochemistry, Microbiology and Bioinformatics
SDG 09. Industry, Innovation and Infrastructure
SDG 03. Good Health and Well-Being
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