Hsp90α/β associates with the GSK3β/axin1/phospho-β-catenin complex in the human MCF-7 epithelial breast cancer model:

Cooper, Leanne C; Prinsloo, Earl; Edkins, Adrienne L; Blatch, Gregory L

Title: Hsp90α/β associates with the GSK3β/axin1/phospho-β-catenin complex in the human MCF-7 epithelial breast cancer model:
Creator: Cooper, Leanne C
Creator: Prinsloo, Earl
Creator: Edkins, Adrienne L
Creator: Blatch, Gregory L
Date Issued: 2011
Date: 2011
Type: text
Type: article
Identifier: http://hdl.handle.net/10962/165096
Identifier: vital:41208
Identifier: DOI: 10.1016/j.bbrc.2011.08.136
Description: Hsp90α/β, the signal transduction chaperone, maintains intracellular communication in normal, stem, and cancer cells. The well characterised association of Hsp90α/β with its client kinases form the framework of multiple signalling networks. GSK3β, a known Hsp90α/β client, mediates β-catenin phosphorylation as part of a cytoplasmic destruction complex which targets phospho-β-catenin to the 26S proteasome. The canonical Wnt/β-catenin pathway promotes stem cell self-renewal as well as oncogenesis. The degree of Hsp90α/β involvement in Wnt/β-catenin signalling needs clarification. Here, we describe the association of Hsp90α/β with GSK3β, β-catenin, phospho-β-catenin and the molecular scaffold, axin1, in the human MCF-7 epithelial breast cancer cell model using selective inhibition of Hsp90α/β, confocal laser scanning microscopy and immunoprecipitation. Our findings suggest that Hsp90α/β modulates the phosphorylation of β-catenin by interaction in common complex with GSK3β/axin1/β-catenin.
Format: 5 pages
Format: pdf
Language: English
Relation: Biochemical and biophysical research communications
Relation: Cooper, L.C., Prinsloo, E., Edkins, A.L. and Blatch, G.L., 2011. Hsp90α/β associates with the GSK3β/axin1/phospho-β-catenin complex in the human MCF-7 epithelial breast cancer model. Biochemical and biophysical research communications, 413(4), pp.550-554
Relation: Biochemical and biophysical research communications volume 413 number 4 550 554 October 2011 1090-2104
Rights: Publisher
Rights: Use of this resource is governed by the terms and conditions of the Elsevier Terms and Conditions Statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)

Hits: 2045
Visitors: 2146
Downloads: 114

Collections

Prinsloo, Earl (Dr)

Edkins, Adrienne Lesley (Associate Prof)

Blatch, Gregory Lloyd (Prof)

SDG 09. Industry, Innovation and Infrastructure

RU Department of Biochemistry, Microbiology and Bioinformatics

SDG 03. Good Health and Well-Being

		Thumbnail	File	Description	Size	Format
View Details Download			SOURCE1	Hsp90αβ associates with the GSK3β.pdf	689 KB	Adobe Acrobat PDF	View Details Download