- Title
- Knockdown of Hop downregulates RhoC expression, and decreases pseudopodia formation and migration in cancer cell lines:
- Creator
- Willmer, Tarryn
- Creator
- Contu, Lara
- Creator
- Blatch, Gregory L
- Creator
- Edkins, Adrienne L
- Date Issued
- 2013
- Date
- 2013
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/165196
- Identifier
- vital:41217
- Identifier
- DOI: 10.1016/j.canlet.2012.09.021
- Description
- The Hsp90/Hsp70 organising protein (Hop) is a co-chaperone that mediates the interaction of Hsp90 and Hsp70 molecular chaperones during assembly of Hsp90 complexes in cells. Formation of Hsp90 complexes is a key intermediate step in the maturation and homeostasis of oncoproteins and several hormone receptors. In this paper, we demonstrate that knockdown of Hop decreased migration of Hs578T and MDA-MB-231 breast cancer cells. Hop was identified in isolated pseudopodia fractions; it colocalised with actin in lamellipodia, and co-sedimented with purified actin in vitro. Knockdown of Hop caused a decrease in the level of RhoC GTPase, and significantly inhibited pseudopodia formation in Hs578T cells. Our data suggest that Hop regulates directional cell migration by multiple unknown mechanisms.
- Format
- 9 pages
- Format
- Language
- English
- Relation
- Cancer letters
- Relation
- Willmer, T., Contu, L., Blatch, G.L. and Edkins, A.L., 2013. Knockdown of Hop downregulates RhoC expression, and decreases pseudopodia formation and migration in cancer cell lines. Cancer letters, 328(2), pp.252-260
- Relation
- Cancer letters volume 328 number 2 252 260 January 2013 1872-7980
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Elsevier Terms and Conditions Statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)
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Edkins, Adrienne Lesley (Associate Prof)
Blatch, Gregory Lloyd (Prof)
SDG 03. Good Health and Well-Being
RU Department of Biochemistry, Microbiology and Bioinformatics
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