- Title
- Development and Validation of a Stability-indicating RP-HPLC Method Using Quality by Design for Estimating Captopril
- Creator
- Veerubhotla, Krishna
- Creator
- Walker, Roderick B
- Subject
- To be catalogued
- Date Issued
- 2019
- Date
- 2019
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/183525
- Identifier
- vital:44003
- Identifier
- xlink:href="10.4172/pharmaceutical-sciences.1000478"
- Description
- The applicability of a quality by design framework for the development of a sensitive, simple and selective, stability-indicating reversed-phase high-performance liquid chromatography analytical method for the analysis of captopril was investigated. Design of experiments using a central composite design approach was used for method development. Twenty experimental runs were performed with acetonitrile content ranging between 28 and 36 % v/v, pH from 2.8 to 3.6 and temperature between 22° and 32°. The experimental data obtained was used to derive a quadratic model for the retention time of captopril. The optimized method produced sharp peaks with good resolution (>2) for captopril and the internal standard with retention times of 3.1 and 6.2 min, respectively. The experimental data revealed that acetonitrile content in the mobile phase and pH are significant factors that affect the retention time and resolution of captopril. Normal probability plots revealed that the residual and predicted data fall approximately on a straight line, indicating that the experimental error for these studies was evenly distributed suggesting that the model could be used to navigate the design space. This approach is useful to expedite method development and optimization activities in analytical laboratories.
- Format
- computer
- Format
- online resource
- Format
- application/pdf
- Format
- 1 online resource (12 pages)
- Format
- Publisher
- Indian Pharmaceutical Association
- Language
- English
- Relation
- Indian Journal of Pharmaceutical Sciences
- Relation
- Veerubhotla, K. and Walker, R.B., 2019. Development and Validation of a Stability-indicating RP-HPLC Method Using Quality by Design for Estimating Captopril. Indian Journal of Pharmaceutical Sciences, 81(1), pp.45-56
- Relation
- Indian Journal of Pharmaceutical Sciences volume 81 number 1 p. 45 2019 1998-3743
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Indian Journal of Pharmaceutical Sciences Statement (https://www.ijpsonline.com/aboutus.html )
- Rights
- Open Access
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Collections
Walker, Roderick Bryan (Prof)
SDG 03. Good Health and Well-Being
RU Department of Pharmacy
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | Development and Validation of a Stability-indicating RP-HPLC Method Using Quality by Design for Estimating Captopril.pdf | 924 KB | Adobe Acrobat PDF | View Details Download |