- Title
- Electrochemical impedimetric immunosensor for the detection of measles-specific IgG antibodies after measles infections
- Creator
- Mashazi, Philani N
- Creator
- Tetyana, Phumlani
- Creator
- Vilakazi, Sibulelo
- Creator
- Nyokong, Tebello
- Subject
- To be catalogued
- Date Issued
- 2013
- Date
- 2013
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/241677
- Identifier
- vital:50960
- Identifier
- xlink:href="https://doi.org/10.1016/j.bios.2013.04.028"
- Description
- The detection of measles-specific primary antibodies (IgG) using electrochemical impedimetric immunosensors is reported. The optimum conditions for electrode saturation were reached after 40 min for 1 μg ml−1 antibody concentrations. Surface roughness using AFM increased with each immobilization or antigen-antibody reaction step clearly confirming the surface modification and recognition between antigen and antibody. The human serum (HS) and new-born calf serum (NCS) spiked with antigen-specific antibody were studied to mimic the real sample analysis. The HS and NCS sera containing antibodies due to measles exhibited correlation between the increasing antibody serum concentrations and the charge-transfer resistance (electrochemically measured). This work clearly showed the potential use of impedance as the preferred electrochemical method for detecting measles-antibodies in label-free manner.
- Format
- computer
- Format
- online resource
- Format
- application/pdf
- Format
- 1 online resource (7 pages)
- Format
- Publisher
- Elsevier
- Language
- English
- Relation
- Biosensors and Bioelectronics
- Relation
- Mashazi, P., Tetyana, P., Vilakazi, S. and Nyokong, T., 2013. Electrochemical impedimetric immunosensor for the detection of measles-specific IgG antibodies after measles infections. Biosensors and Bioelectronics, 49, pp.32-38
- Relation
- Biosensors and Bioelectronics volume 49 p. 32 2013 1873-4235
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Elsevier Terms and Conditions Statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)
- Rights
- Closed Access
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Mashazi, Philani N (Associate Prof)
RU DST/NRF SARChI for Medicinal Chemistry and Nanotechnology
Nyokong, Tebello (Distinguished Professor)
RU Department of Chemistry
SDG 03. Good Health and Well-Being
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | Electrochemical impedimetric immunosensor for the detection of measles-specific IgG antibodies.pdf | 713 KB | Adobe Acrobat PDF | View Details Download |