- Title
- Evaluation of anti-diabetic activity of selected red marine macro-algae found along South African coastal areas
- Creator
- Bambo, Karabo https://orcid.org/0000-0002-3721-2424
- Subject
- Marine algae
- Subject
- Phytochemicals
- Date Issued
- 2021-04
- Date
- 2021-04
- Type
- Master's theses
- Type
- text
- Identifier
- http://hdl.handle.net/10353/22533
- Identifier
- vital:52405
- Description
- Marine macro-algae are a good source of potential primary and secondary metabolites. Phytochemicals of the samples were extracted using methanol and water. This study evaluated the phytochemical constituents, antioxidant activity and antidiabetic activity of selected red marine macro-algae sampled from Gqeberha, South Africa. The species collected were identified as Gelidium pristoides, Scinaia furcellata and Portieria tripinita using DNA barcoding. Results showed the presence of several phytochemicals such as saponins, phenols, flavonols, tannins, proanthocyanidins and flavonoids. Methanol was a more effective polyphenol extraction solvent than water. Gelidium pristoides (H2O) was observed to contain the highest phenol and proanthocyanidin content, which correlated to Gelidium pristoides (H2O) having the greatest FRAP and DPPH radical scavenging activity IC50 at 0.227. Portieria tripinita (MeOH) extract had the greatest flavonoid content. Flavonol content was high for Scinaia furcellata while the highest tannin content was observed in both H2O and MeOH extracts of Gelidium pristoides. All the selected marine macro-algae species showed concentration-dependent reducing power. The extract with the highest ABTS scavenging activity was Gelidium pristoides (MeOH) IC50 at 0.227. Portieria tripinita was the only species that showed Nitric oxide scavenging activity, with (H2O) extract showing the highest activity IC50 at 3.052. Only MeOH extracts of Gelidium pristoides and Scinaia furcellata showed very slight α-amylase inhibition, however, all extracts successfully inhibited the activity of α-glucosidase with the exception of the H2O extracts of Scinaia furcellata. All crude extracts of Gelidium pristoides, Scinaia furcellata and Portieria tripinita showed no significant/weak effect on glucose uptake and utilization by C3A cells following 24 hours of treatment, with the exception of the aqueous extracts of Scinaia furcellata at the highest concentration of 100 μg/ml. The in-vitro MTT cytotoxicity assay revealed that only MeOH extracts of Gelidium pristoides and Scinaia furcellata had a 15 significant toxic effect on C3A hepatocytes at concentrations of 100 μg/ml and 25μg/ml, respectively. Portieria tripinita aqueous extract showed cytotoxicity when evaluated using Hoechst 33342/Propidium iodide (PI) dual staining.
- Description
- Thesis (MSc) -- Faculty of Science and Agriculture, 2021
- Format
- computer
- Format
- online resource
- Format
- application/pdf
- Format
- 1 online resource (iii, 101 pages)
- Format
- Publisher
- University of Fort Hare
- Publisher
- Faculty of Science and Agriculture
- Language
- English
- Rights
- rights holder
- Rights
- All Rights Reserved
- Rights
- Open Access
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UFH Department of Biochemistry & Microbiology
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | Msc dissertation Bambo K 09 APR 2022_1.pdf | 2 MB | Adobe Acrobat PDF | View Details Download |