Cellulases (CMCases) and polyphenol oxidases from thermophilic Bacillus spp

Mayende, Lungisa; Wilhelmi, Brendan S; Pletschke, Brett I

Title: Cellulases (CMCases) and polyphenol oxidases from thermophilic Bacillus spp
Creator: Mayende, Lungisa
Creator: Wilhelmi, Brendan S
Creator: Pletschke, Brett I
Date Issued: 2006
Date: 2006
Type: Article
Identifier: vital:6462
Identifier: http://hdl.handle.net/10962/d1005791
Identifier: http://dx.doi.org/10.1016/j.soilbio.2006.03.019
Description: In composting, organic matter is degraded by cellulases and ligninolytic enzymes at temperatures typically above 50 °C. This study isolated thermophilic microorganisms from a compost system that were then screened for cellulase and polyphenol oxidase activity. Temperature optima for the cellulases and polyphenol oxidases were determined as 70 and 40 °C, respectively. Maximal cellulase activity was determined as 1.333 mg glucose released ml[superscript −1] min[superscript −1]. Maximal polyphenol oxidase activity attained was 5.111 nmol phenol ml[superscript −1] min[superscript −1]. Cellulases were found to be stable over a period of 1 h. The isolated compost microorganisms were identified as strains of Bacillus using 16S ribosomal DNA sequence analysis.
Format: 6 pages
Format: pdf
Language: English
Relation: Mayende, L. and Wilhelmi, Brendan Shane and Pletschke, B.I. (2006) Cellulases (CMCases) and polyphenol oxidases from thermophilic Bacillus spp. isolated from compost. Soil Biology and Biochemistry, 38 (9). pp. 2963-2966. Available: http://dx.doi.org/10.1016/j.soilbio.2006.03.019

Hits: 830
Visitors: 986
Downloads: 178

Collections

Wilhelmi, Brendan (Dr)

Pletschke, Brett (Prof)

RU Department of Biochemistry, Microbiology and Bioinformatics

SDG 15. Life On Land

		Thumbnail	File	Description	Size	Format
View Details Download			SOURCEPDF	PDF	136 KB	Adobe Acrobat PDF	View Details Download