- Title
- Genetic and biological characterisation of a novel Plutella xylostella granulovirus, PlxyGV-SA
- Creator
- Abdulkadir, Fatima
- Creator
- Knox, Caroline M
- Creator
- Marsberg, Tamryn
- Creator
- Hill, Martin P
- Creator
- Moore, Sean D
- Subject
- To be catalogued
- Date Issued
- 2015
- Date
- 2015
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/417971
- Identifier
- vital:71498
- Identifier
- xlink:href="https://doi.org/10.1007/s10526-015-9666-3"
- Description
- Plutella xylostella granulovirus (PlxyGV) has been isolated from insect populations in many countries and is considered a potential biopesticide for sustainable control of P. xylostella (L.) (Lepidoptera: Plutellidae). Several PlxyGV isolates have been genetically characterised, and the full genome sequence of PlxyGV-Japan is available for comparison with novel isolates. A South African PlxyGV was recently recovered from an overcrowded laboratory P. xylostella colony and identified as a genetically distinct isolate by sequencing of the granulin gene and restriction endonuclease (REN) analysis of genomic DNA. In this report, PlxyGV-SA was further characterised by PCR amplification and sequencing of egt, lef-8 and lef-9 genes, and several amino acid substitutions were observed. The PstI REN profile of PlxyGV-SA was different from that of PlxyGV-Japan in terms of band size and number, thereby confirming its novel genetic identity. Surface dose bioassays showed that PlxyGV-SA is pathogenic to neonate but not late instar larvae at the same and higher virus doses, indicating that a biopesticide should be targeted at early larval stages in the field.
- Format
- computer
- Format
- online resource
- Format
- application/pdf
- Format
- 1 online resource (9 pages)
- Format
- Publisher
- SpringerLink
- Language
- English
- Relation
- BioControl
- Relation
- Abdulkadir, F., Knox, C., Marsberg, T., Hill, M.P. and Moore, S.D., 2015. Genetic and biological characterisation of a novel Plutella xylostella granulovirus, PlxyGV-SA. BioControl, 60, pp.507-515
- Relation
- BioControl volume 60 p. 507 2015 1573-8248
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the SpringerLink Terms of Use Statement ( https://link.springer.com/termsandconditions)
- Rights
- Closed Access
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Collections
Knox, Caroline M (Prof)
Hill, Martin Patrick (Distinguished Professor)
RU DST/NRF SARChI for Insects in Sustainable Agricultural Ecosystems
RU Department of Biochemistry, Microbiology and Bioinformatics
SDG 15. Life On Land
RU Department of Zoology and Entomology
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | Genetic and biological characterisation of a novel Plutella xylostella granulovirus, PlxyGV-SA.pdf | 737 KB | Adobe Acrobat PDF | View Details Download |