- Title
- Partial Purification and Characterization of Endoxylanase from a fungus, Leohumicola incrustata
- Creator
- Adeoyo, Olusegun R
- Creator
- Pletschke, Brett I
- Creator
- Dames, Joanna F
- Subject
- To be catalogued
- Date Issued
- 2021
- Date
- 2021
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/440393
- Identifier
- vital:73779
- Identifier
- 10.4314/br.v19i1.2
- Description
- Xylanases are glycoside hydrolases (GH) that degrade β-1, 4-xylan, a linear polysaccharide found as hemicellulose in cell wall of plants. Endoxylanase (Endo-1, 4-β-xylanase, EC 3.2. 1.8) randomly catalyses xylan to produce varying short xylooligosaccharides (XOS). This study aimed to determine the characteristics of a partially purified endoxylanase from Leohumicola incrustata. Enzyme production was carried out using beechwood (BW) xylan, after which the cell-free crude filtrate was concentrated using the ammonium sulphate precipitation method. The hydrolysed products were analysed by thin-layer chromatography (TLC) and zymography. The result showed that the enzyme produced varying smaller-sized linear xylooligosaccharides with R f values corresponding to those of xylobiose, xylotriose, xylotetraose, xylopentaose, xylohexaose and other higher oligomers. The endoxylanase had a molecular mass of 72 kDa. The enzyme is stable in the presence of K+, Na+, Ca 2+, Fe 2+, Mg 2+, Zn 2+, Co 2+, pH of 5.0 and temperature of 37 o C. However, the activity gradually decreased after 60 min at 50 o C and retained over 69% activity after 120 min, while at 60 and 70 o C, the enzyme activity sharply decreased (pre-incubation periods). Endoxylanase from L. incrustata is comparable to those of other microorganisms and should be considered an attractive candidate for future industrial applications.
- Format
- 10 pages
- Format
- Language
- English
- Relation
- Bio-Research
- Relation
- Adeoyo, O.R., Pletschke, B.I. and Dames, J.F., 2021. Partial Purification and Characterization of Endoxylanase from a fungus, Leohumicola incrustata. Bio-Research, 19(1), pp.1192-1201
- Relation
- Bio-Research volume 19 number 1 1192 1201 2021
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Bio-Research Statement (https://www.ajol.info/index.php/br)
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Collections
Dames, Joanna Felicity (Prof)
Pletschke, Brett (Prof)
SDG 09. Industry, Innovation and Infrastructure
RU Department of Biochemistry, Microbiology and Bioinformatics
SDG 15. Life On Land
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