- Title
- Knowledge, awareness, and molecular epidemiology of fasciolosis in dairy cattle slaughtered in three commercial abattoirs in Eastern Cape Province, South Africa
- Creator
- Mpisana, Zuko
- Subject
- Farm management -- South Africa -- Eastern Cape
- Subject
- Dairy cattle -- South Africa -- Eastern Cape
- Subject
- Fascioliasis
- Date Issued
- 2023-06
- Date
- 2023-06
- Type
- Doctoral theses
- Type
- text
- Identifier
- http://hdl.handle.net/10353/28173
- Identifier
- vital:73792
- Description
- Flukes and snail-borne diseases present significant challenges to livestock production, particularly impacting the dairy industry in the Eastern Cape Province, South Africa. Despite the challenges faced by the dairy sector, the level of knowledge and awareness among dairy farm personnel regarding the prevalence of bovine fasciolosis is unknown in Eastern Cape Province. Additionally, the genomic sequence of Fasciola spp. in dairy cattle slaughtered under abattoir conditions remains undisclosed. The primary objective of the titled study is to evaluate the knowledge, awareness, and molecular epidemiology of fasciolosis in dairy cattle slaughtered in three commercial abattoirs in the Eastern Cape province of South Africa. A structured questionnaire was prepared and randomly administered to 152 dairy farm personnel to investigate the knowledge and awareness of the aetiology, risk factors, and clinical signs of bovine fasciolosis among dairy farm personnel in the Eastern Cape Province, South Africa. A higher proportion of respondents from coastal regions were knowledgeable about the aetiology P equals 0.001 and the intermediate host P equals 0.000 of fasciolosis than those from the inland farms. A higher proportion of dairy farm personnel were not knowledgeable about the clinical signs of fasciolosis P equals 0.000 and the zoonotic potential P equals 0.001 of the disease. All farm personnel knew that season P 0.001 is a risk factor associated with snail-borne disease and occur throughout the year. However, the majority 63.2 percent of farm personnel agreed that fasciolosis was more prevalent in the summer followed by spring 27.6 percent, autumn 4.7 percent and winter 4.7 percent. Experiment 2 examined the body condition scores, fluke intensity, liver pathology, and carcass quality of various dairy cattle genotypes affected by Fasciola spp. infection, within three high-capacity abattoirs located in the Eastern Cape Province, South Africa. Milder infections were significantly noted to occur at a higher rate P 0.05 in Cross-bred cattle 46.9 percent followed by Holstein Friesian 39.5 percent and Jersey 30.9 percent cattle. Conversely, moderate infections were more prevalent P 0.05 among Jersey cattle 28.0 percent followed by Holstein Friesian 15.4 percent and Cross bred 1.9 percent cattle. Severe infections were more frequent P 0.05 in Holstein-Friesian 45.1 percent, followed by Jersey 41.9 percent and then Cross-bred 31.7 percent cattle. Heavy infections were observed in cattle with poor body condition scores in CA3. Mild infections were observed more in cattle with moderate body condition scores in CA3 and CA1 and less in CA2. Chapter 5 compared the detection rate of Fasciola infections using four different methods real-time quantitative polymerase chain reaction qPCR faecal sedimentation, antibody enzyme linked immunosorbent assay Ab ELISA and post-mortem liver examination among naturally infected cattle slaughtered at the abattoirs in the Eastern Cape Province, South Africa. A total of 145 slaughter cattle were screened for Fasciola infestation by liver examination, and corresponding samples from individual animals were analysed in the laboratory using Ab ELISA faecal sedimentation, and qPCR. The detection rate by liver inspection gold standard was significantly P 0.0001 higher than that by sedimentation and Ab ELISA but not significantly P 0.05 different from that by qPCR P equals 0.198. Post-mortem liver examination 94.5 percent and qPCR 90.4 percent had higher detection rate compared to Ab ELISA 22.6 percent and sedimentation 3.53 percent, respectively. The agreement between Ab ELISA and sedimentation was poor to slight kappa equals to minus 0.09 and with qPCR it was 0.008. The fourth experiment investigated genomic sequence of Fasciola spp. from faecal sample of slaughtered dairy cattle in Eastern Cape Province South Africa. Sequential and phylogenetic analyses were conducted to detect the presence of Fasciola spp. from the faecal samples obtained at the high throughput abattoirs. Fasciola hepatica and F. gigantica ITS 2 sequences were obtained by amplifying the 364 bp and 300 bp genes, respectively using species specific conventional PCR assays followed by cloning and sequencing. The phylogenetic tree revealed the presence of Fasciola hepatica as the only aetiological agent infecting dairy cattle in the Eastern Cape Province South Africa. The study concluded that dairy farm personnel lacks knowledge about zoonotic potentials, aetiological agents of fasciolosis high fluke intensity and its impact on carcasses sensitivity and suitability of detection methods. The study observed Fasciola hepatica as the only trematode spp. present in Eastern Cape Province South Africa.
- Description
- Thesis (PhD) -- Faculty of Science and Agriculture, 2023
- Format
- computer
- Format
- online resource
- Format
- application/pdf
- Format
- 1 online resource (xviii, 230 leaves)
- Format
- Publisher
- University of Fort Hare
- Publisher
- Faculty of Science and Agriculture
- Language
- English
- Rights
- University of Fort Hare
- Rights
- All Rights Reserved
- Rights
- Open Access
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Collections
UFH Department of Livestock & Pasture Science
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | Mpisana Doctoral Thesis.pdf | 77 MB | Adobe Acrobat PDF | View Details Download |