Development of high yielding strain of Pleurotus tuber-regium fructification, nutritional and phylogenetic studies

Bamigboye, Comfort O; Oloke, Julius K; Dames, Joanna F

Title: Development of high yielding strain of Pleurotus tuber-regium fructification, nutritional and phylogenetic studies
Creator: Bamigboye, Comfort O
Creator: Oloke, Julius K
Creator: Dames, Joanna F
Subject: To be catalogued
Date Issued: 2019
Date: 2019
Type: text
Type: article
Identifier: http://hdl.handle.net/10962/448595
Identifier: vital:74745
Identifier: https://doi.org/10.1007/s13197-019-03786-7
Description: Mushrooms are nutritionally rich, healthy and medicinal. Pleurotus tuber-regium (Fr.) is one of the nutritious medicinal mushroom found in the tropics and subtropics, but with history of slow growth and low sclerotia yield. In this study, mutants were created by mycelia exposure to ultraviolet irradiation (at a wavelength of 254 nm and a distance of 45 cm), for 3 h and sub-cultured at 30 min interval. The DNA from the wild and mutant strains were extracted, PCR amplified and sequenced. A phylogenetic tree was constructed to show the degree of similarity and differences between the wild and the mutant strains. Fructification studies were conducted on Rhodes grass straw and sawdust to determine the viability of the mutant strains and any nutritional improvement. The wild strain of P. tuber-regium and mutant produced at 30 min (Pt30) cultivated on sawdust and Rhodes straw, yielded sclerotia with biological efficiency of 8.8 and 47.6% respectively. Proximate analysis of the sclerotium showed that the mutant, Pt30, had improved nutritional compositions compared to the wild strain with a total non-structural carbohydrate concentration of 2.41 g as against 0.93 g. Conclusively in this study, better strains of P. tuber-regium were produced with faster growth rate, higher mycelia ramification rate on lignocellulosic substrate and a higher sclerotia yield than the wild P. tuber-regium. It was also established that mutagenesis is capable of improving P. tuber-regium for a successful commercial venture in sclerotia production.
Format: 12 pages
Format: pdf
Language: English
Relation: Journal of food science and technology
Relation: Bamigboye, C.O., Oloke, J.K. and Dames, J.F., 2019. Development of high yielding strain of Pleurotus tuber-regium: fructification, nutritional and phylogenetic studies. Journal of food science and technology, 56(8), pp.3597-3608
Relation: Journal of food science and technology volume 56 number 8 3597 3608 2019
Rights: Publisher
Rights: Use of this resource is governed by the terms and conditions of the Springer Terms of Use Statement ( https://link.springer.com/termsandconditions)

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