Interaction of silver nanoparticles with catechol O-methyltransferase: Spectroscopic and simulation analyses

Usman, Aminu; Lobb, Kevin A; Pletschke, Brett I; Whiteley, Christopher G; Wilhelmi, Brendan S

Title: Interaction of silver nanoparticles with catechol O-methyltransferase: Spectroscopic and simulation analyses
Creator: Usman, Aminu
Creator: Lobb, Kevin A
Creator: Pletschke, Brett I
Creator: Whiteley, Christopher G
Creator: Wilhelmi, Brendan S
Subject: To be catalogued
Date Issued: 2021
Date: 2021
Type: text
Type: article
Identifier: http://hdl.handle.net/10962/451095
Identifier: vital:75018
Identifier: xlink:href=" https://doi.org/10.1016/j.bbrep.2021.101013"
Description: Catechol O-methyltransferase, an enzyme involved in the metabolism of catechol containing compounds, catalyzes the transfer of a methyl group between S-adenosylmethionine and the hydroxyl groups of the catechol. Furthermore it is considered a potential drug target for Parkinson’s disease as it metabolizes the drug levodopa. Consequently inhibitors of the enzyme would increase levels of levodopa. In this study, absorption, fluorescence and infrared spectroscopy as well as computational simulation studies investigated human soluble catechol Omethyltransferase interaction with silver nanoparticles. The nanoparticles form a corona with the enzyme and quenches the fluorescence of Trp143. This amino acid maintains the correct structural orientation for the catechol ring during catalysis through a static mechanism supported by a non-fluorescent fluorophore–nanoparticle complex. The enzyme has one binding site for AgNPs in a thermodynamically spontaneous binding driven by electrostatic interactions as confirmed by negative ΔG and ΔH and positive ΔS values. Fourier transform infrared spectroscopy within the amide I region of the enzyme indicated that the interaction causes relaxation of its β− structures, while simulation studies indicated the involvement of six polar amino acids. These findings suggest AgNPs influence the catalytic activity of catechol O-methyltransferase, and therefore have potential in controlling the activity of the enzyme.
Format: computer
Format: online resource
Format: application/pdf
Format: 1 online resource (9 pages)
Format: pdf
Publisher: Elsevier
Language: English
Relation: Biochemistry and Biophysics Reports
Relation: Usman, A., Lobb, K., Pletschke, B.I., Whiteley, C.G. and Wilhelmi, B.S., 2021. Interaction of silver nanoparticles with catechol O-methyltransferase: Spectroscopic and simulation analyses. Biochemistry and Biophysics Reports, 26, p.101013
Relation: Biochemistry and Biophysics Reports volume 26 p. 101013 2021 2405-5808
Rights: Publisher
Rights: Use of this resource is governed by the terms and conditions of the Elsevier Terms and Conditions Statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)
Rights: Open Access

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Whiteley, Christopher (Emeritus Prof)

Wilhelmi, Brendan (Dr)

Lobb, Kevin Allan (Assoc Prof)

Pletschke, Brett (Prof)

RU Department of Chemistry

RU Department of Biochemistry, Microbiology and Bioinformatics

SDG 03. Good Health and Well-Being

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