Detection of the in vitro modulation of Plasmodium falciparum Arf1 by Sec7 and ArfGAP domains using a colorimetric plate-based assay:

Swart, Tarryn; Khan, Farrah D; Ntlantsana, Apelele; Laming, Dustin; Veale, Clinton G L; Przyborski, Jude M; Edkins, Adrienne L; Hoppe, Heinrich C

Title: Detection of the in vitro modulation of Plasmodium falciparum Arf1 by Sec7 and ArfGAP domains using a colorimetric plate-based assay:
Creator: Swart, Tarryn
Creator: Khan, Farrah D
Creator: Ntlantsana, Apelele
Creator: Laming, Dustin
Creator: Veale, Clinton G L
Creator: Przyborski, Jude M
Creator: Edkins, Adrienne L
Creator: Hoppe, Heinrich C
Date Issued: 2020
Date: 2020
Type: text
Type: article
Identifier: http://hdl.handle.net/10962/165418
Identifier: vital:41242
Identifier: https://0-doi.org.wam.seals.ac.za/10.1038/s41598-020-61101-3
Description: The regulation of human Arf1 GTPase activity by ArfGEFs that stimulate GDP/GTP exchange and ArfGAPs that mediate GTP hydrolysis has attracted attention for the discovery of Arf1 inhibitors as potential anti-cancer agents. The malaria parasite Plasmodium falciparum encodes a Sec7 domain-containing protein - presumably an ArfGEF - and two putative ArfGAPs, as well as an Arf1 homologue (PfArf1) that is essential for blood-stage parasite viability. However, ArfGEF and ArfGAP-mediated activation/deactivation of PfArf1 has not been demonstrated.
Format: 12 pages
Format: pdf
Language: English
Relation: Scientific reports
Relation: Swart, T., Khan, F.D., Ntlantsana, A., Laming, D., Veale, C.G., Przyborski, J.M., Edkins, A.L. and Hoppe, H.C., 2020. Detection of the in vitro modulation of Plasmodium falciparum Arf1 by Sec7 and ArfGAP domains using a colorimetric plate-based assay. Scientific reports, 10(1), pp.1-11
Relation: Scientific reports volume 10 number 1 1 11 March 2020 2045-2322
Rights: Publisher
Rights: Use of this resource is governed by the terms and conditions of the Nature Terms and Conditions Statement (https://www.nature.com/info/terms-and-conditions)

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RU DST/NRF SARChI for Molecular and Cellular Biology of the Eukaryotic Stress Response

Edkins, Adrienne Lesley (Associate Prof)

RU Centre for Chemico- and Biomedicinal Research (CCBR)

Hoppe, Heinrich C (Prof)

RU Department of Chemistry

RU Department of Biochemistry, Microbiology and Bioinformatics

SDG 03. Good Health and Well-Being

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