Effect of post-slaughter handling on physico-chemical and microbiological quality of red meat along the distribution chain in the Eastern Cape Province, South Africa
- Authors: Rani, Zikhona Theodora
- Date: 2015
- Subjects: Meat -- Microbiology Meat -- Transportation -- South Africa -- Eastern Cape Meat -- Quality -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/2490 , vital:27875
- Description: The broad objective of the study was to investigate the effect of post-slaughter handling in the distribution chain on red meat quality and safety. A survey was conducted among 300 consumers and 100 meat handlers in five different municipalities (Buffalo City, Nkonkobe, Ngqushwa, Lukhanje and Amahlathi) in the Eastern Cape Province of South Africa to investigate their perceptions on meat quality and safety, together with challenges faced by meat handlers during the distribution of meat from the abattoir to retailers. The microbiological profile and physico-chemical quality of red meat at different stages of the abattoir to retail outlets in the distribution chain were also determined. Swabs (n=216) and meat samples (n=450) were collected from beef, pork and mutton carcasses during the loading process of carcasses into trucks at the abattoir, when offloading carcasses at the supply points and during marketing. Physico-chemical qualities such as colour (L* - lightness, b* - redness and a* - yellowness) and meat pH measurements were taken at each point. To determine the microbiological profiles of the carcasses, four microbiological parameters were considered: Total bacteria count (general bacteria), coliform count (related to hygiene and indicator for pathogens), Escherichia coli (Gram-negative pathogen) and Staphylococcus aureus (Gram-positive pathogen). Two types of packaging (vacuum and overwrapping) were used to determine their effect on shelf-life and microbiological quality of red meat under the normal marketing conditions over a storage duration of 15 days. The results from the study showed low awareness of consumers about the pathogenic diseases which arise from meat. A strong significant association (p ˂ 0.05) between educational status and awareness on meat safety was observed. Most of the consumers perceived that quality goes beyond safety such that 35.6 percent of the respondents indicated that they did not have a problem with consuming spoiled meat, whilst the remaining 64.4 percent indicated that they would r eject spoiled meat. Although retailers indicated that they take meat safety into consideration in their shops, 92 percent of the retailers revealed that they do not perform microbial assessment of meat in their shops. A series of loading and off-loading, temperature fluctuations, environmental temperatures and ques during offloading were reported as the major challenges during transportation of carcasses from the abattoir to the supply points. The microbial counts were significantly (p<0.05) higher in samples from the commercial abattoir than in those from the communal abattoir. Escherichia coli was the predominant microbial contaminant in the samples from both abattoirs. When following the chain, total bacterial count (TBC), coliform count (CC) and the levels of E. coli contamination increased progressively between the loading and the off-loading points (5.1 to 7.9 log10 CFU/cm2; 5.0 to 5.6 log10 CFU/cm2 and 2.7 to 3.7 log10 CFU/cm2, respectively). The storage period, meat type, distance during transportation and temperature were found to have a significant impact on the microbial levels during the distribution of carcasses. Distribution stage had a significant effect (p<0.05) on some of the physico- chemical meat quality attributes and differences in the lightness (L*) and redness (a*) values between the loading, off-loading and display points were observed. Consumers perceived retailer class as one of the factors influencing meat quality, but according to the instrumental measurements retailer class did not have a significant effect on physico-chemical meat quality. However, distance and storage duration significantly (p<0.05) affected (L*) and (a*) values in the meat during distribution chain. Vacuum and overwrapping packaging significantly affected (p<0.05) the shelf life of meat. Therefore, it was concluded that post-slaughter handling during the distribution chain affects the physico- chemical, microbiological and shelf-life of meat.
- Full Text:
- Date Issued: 2015
- Authors: Rani, Zikhona Theodora
- Date: 2015
- Subjects: Meat -- Microbiology Meat -- Transportation -- South Africa -- Eastern Cape Meat -- Quality -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/2490 , vital:27875
- Description: The broad objective of the study was to investigate the effect of post-slaughter handling in the distribution chain on red meat quality and safety. A survey was conducted among 300 consumers and 100 meat handlers in five different municipalities (Buffalo City, Nkonkobe, Ngqushwa, Lukhanje and Amahlathi) in the Eastern Cape Province of South Africa to investigate their perceptions on meat quality and safety, together with challenges faced by meat handlers during the distribution of meat from the abattoir to retailers. The microbiological profile and physico-chemical quality of red meat at different stages of the abattoir to retail outlets in the distribution chain were also determined. Swabs (n=216) and meat samples (n=450) were collected from beef, pork and mutton carcasses during the loading process of carcasses into trucks at the abattoir, when offloading carcasses at the supply points and during marketing. Physico-chemical qualities such as colour (L* - lightness, b* - redness and a* - yellowness) and meat pH measurements were taken at each point. To determine the microbiological profiles of the carcasses, four microbiological parameters were considered: Total bacteria count (general bacteria), coliform count (related to hygiene and indicator for pathogens), Escherichia coli (Gram-negative pathogen) and Staphylococcus aureus (Gram-positive pathogen). Two types of packaging (vacuum and overwrapping) were used to determine their effect on shelf-life and microbiological quality of red meat under the normal marketing conditions over a storage duration of 15 days. The results from the study showed low awareness of consumers about the pathogenic diseases which arise from meat. A strong significant association (p ˂ 0.05) between educational status and awareness on meat safety was observed. Most of the consumers perceived that quality goes beyond safety such that 35.6 percent of the respondents indicated that they did not have a problem with consuming spoiled meat, whilst the remaining 64.4 percent indicated that they would r eject spoiled meat. Although retailers indicated that they take meat safety into consideration in their shops, 92 percent of the retailers revealed that they do not perform microbial assessment of meat in their shops. A series of loading and off-loading, temperature fluctuations, environmental temperatures and ques during offloading were reported as the major challenges during transportation of carcasses from the abattoir to the supply points. The microbial counts were significantly (p<0.05) higher in samples from the commercial abattoir than in those from the communal abattoir. Escherichia coli was the predominant microbial contaminant in the samples from both abattoirs. When following the chain, total bacterial count (TBC), coliform count (CC) and the levels of E. coli contamination increased progressively between the loading and the off-loading points (5.1 to 7.9 log10 CFU/cm2; 5.0 to 5.6 log10 CFU/cm2 and 2.7 to 3.7 log10 CFU/cm2, respectively). The storage period, meat type, distance during transportation and temperature were found to have a significant impact on the microbial levels during the distribution of carcasses. Distribution stage had a significant effect (p<0.05) on some of the physico- chemical meat quality attributes and differences in the lightness (L*) and redness (a*) values between the loading, off-loading and display points were observed. Consumers perceived retailer class as one of the factors influencing meat quality, but according to the instrumental measurements retailer class did not have a significant effect on physico-chemical meat quality. However, distance and storage duration significantly (p<0.05) affected (L*) and (a*) values in the meat during distribution chain. Vacuum and overwrapping packaging significantly affected (p<0.05) the shelf life of meat. Therefore, it was concluded that post-slaughter handling during the distribution chain affects the physico- chemical, microbiological and shelf-life of meat.
- Full Text:
- Date Issued: 2015
Perceptions of rural consumers and the quality of mutton at purchase points in the Eastern Cape Province, South Africa
- Authors: Rani, Zikhona Theodora
- Date: 2012
- Subjects: Meat -- Quality , Mutton -- Quality , Consumers' preferences , Quality of products , Consumers -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11823 , http://hdl.handle.net/10353/d1007562 , Meat -- Quality , Mutton -- Quality , Consumers' preferences , Quality of products , Consumers -- South Africa -- Eastern Cape
- Description: The objective of the study was to determine perceptions of rural consumers on mutton quality, and the quality of mutton at purchase points in the Eastern Cape Province, South Africa. The study was conducted in five different municipalities (Buffalo City, Nkonkobe, Ngqushwa, Lukhanje and Amahlathi). A survey was conducted where a sample of 215 consumers were randomly selected and interviewed, either at point of purchase or as they left the shops. The survey was not limited to the shoppers only but also extended to households from the villages. Questions on some of the most important meat quality cues were compiled. The physico-chemical quality of mutton purchased from different shops was also determined. Forty different shops and butcheries selling mutton from all the selected municipalities were visited. Different parts of mutton samples were bought. Physico-chemical qualities of mutton such as colour (L* - lightness, b* - redness and a* - yellowness) and meat pH measurements were taken at points of purchase. Cooking loss and tenderness evaluations were later done at the Meat Science laboratory at the University of Fort Hare. The results indicated that price was one of the major factors affecting the purchasing decisions of consumers. Thirty four percent of the consumers preferred mutton as compared to other protein sources, even though they were not buying this type of meat because it was not affordable to them. Both male and female consumers suggested that more sheep farmers need to be established in order to reduce the levels of imported mutton into South Africa. They also highlighted that selection programmes that will result in efficient sheep production and reduced mutton prices need to be implemented. Meat at points of purchase was affected by season resulting in lower lightness (L*24.7±0.49) values in winter and higher (L* 32.2±0.49) in Spring. The class of shop did not have an effect on meat quality attributes. Trotter had high values of lightness (L*30.4±2.78a), redness (a*30.4±2.78a), yellowness (13.1±1.08a), pH (6.3±0.12a), tenderness (24.9±3.69b) and cooking loss (39.5±4.38ab). The number of days from when the meat was put on the shelves to the time when it was purchased for consumption (days to purchase) had a significant (P<0.05) negative correlation with the Warner Braztler Shear Force (WBSF)values and lightness of the meat. Significant negative (P < 0.05) correlations between pH and colour of the meat (L*, a* and b*) were also observed. It was concluded that rural consumers perceive the quality of mutton as the best and that the physico-chemical quality of meat purchased from different shops was different, largely based on the part of meat, meat storage conditions and not necessarily on the class of the shop.
- Full Text:
- Date Issued: 2012
- Authors: Rani, Zikhona Theodora
- Date: 2012
- Subjects: Meat -- Quality , Mutton -- Quality , Consumers' preferences , Quality of products , Consumers -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11823 , http://hdl.handle.net/10353/d1007562 , Meat -- Quality , Mutton -- Quality , Consumers' preferences , Quality of products , Consumers -- South Africa -- Eastern Cape
- Description: The objective of the study was to determine perceptions of rural consumers on mutton quality, and the quality of mutton at purchase points in the Eastern Cape Province, South Africa. The study was conducted in five different municipalities (Buffalo City, Nkonkobe, Ngqushwa, Lukhanje and Amahlathi). A survey was conducted where a sample of 215 consumers were randomly selected and interviewed, either at point of purchase or as they left the shops. The survey was not limited to the shoppers only but also extended to households from the villages. Questions on some of the most important meat quality cues were compiled. The physico-chemical quality of mutton purchased from different shops was also determined. Forty different shops and butcheries selling mutton from all the selected municipalities were visited. Different parts of mutton samples were bought. Physico-chemical qualities of mutton such as colour (L* - lightness, b* - redness and a* - yellowness) and meat pH measurements were taken at points of purchase. Cooking loss and tenderness evaluations were later done at the Meat Science laboratory at the University of Fort Hare. The results indicated that price was one of the major factors affecting the purchasing decisions of consumers. Thirty four percent of the consumers preferred mutton as compared to other protein sources, even though they were not buying this type of meat because it was not affordable to them. Both male and female consumers suggested that more sheep farmers need to be established in order to reduce the levels of imported mutton into South Africa. They also highlighted that selection programmes that will result in efficient sheep production and reduced mutton prices need to be implemented. Meat at points of purchase was affected by season resulting in lower lightness (L*24.7±0.49) values in winter and higher (L* 32.2±0.49) in Spring. The class of shop did not have an effect on meat quality attributes. Trotter had high values of lightness (L*30.4±2.78a), redness (a*30.4±2.78a), yellowness (13.1±1.08a), pH (6.3±0.12a), tenderness (24.9±3.69b) and cooking loss (39.5±4.38ab). The number of days from when the meat was put on the shelves to the time when it was purchased for consumption (days to purchase) had a significant (P<0.05) negative correlation with the Warner Braztler Shear Force (WBSF)values and lightness of the meat. Significant negative (P < 0.05) correlations between pH and colour of the meat (L*, a* and b*) were also observed. It was concluded that rural consumers perceive the quality of mutton as the best and that the physico-chemical quality of meat purchased from different shops was different, largely based on the part of meat, meat storage conditions and not necessarily on the class of the shop.
- Full Text:
- Date Issued: 2012
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