DNA-based identification of Lepidoptera associated with citrus in South Africa
- Marsberg, Tamryn, Hill, Martin P, Moore, Sean D, Timm, Alicia E
- Authors: Marsberg, Tamryn , Hill, Martin P , Moore, Sean D , Timm, Alicia E
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/405782 , vital:70205 , xlink:href="https://hdl.handle.net/10520/EJC167513"
- Description: A number of insects, primarily Lepidoptera, cause damage to citrus in South Africa. A major limitation to the management and control of these pests is their correct identification. The aim of this study was to develop a database of gene sequences to aid in the identification of these Lepidoptera. Multiple specimens of 12 species were sequenced for the ~650 bp of the cytochrome oxidase I gene. These sequence data were supplemented and validated using sequences available in public databases. Results showed that each species could be unambiguously identified, and neighbour-joining analysis based on K2P distances formed highly supported, distinct clusters for each species, i.e. the maximum intraspecific genetic distance was less than that of the minimum interspecific genetic distances. Thus, this data set provides a molecular means to successfully identify the most important Lepidoptera associated with citrus in South Africa.
- Full Text:
- Date Issued: 2015
- Authors: Marsberg, Tamryn , Hill, Martin P , Moore, Sean D , Timm, Alicia E
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/405782 , vital:70205 , xlink:href="https://hdl.handle.net/10520/EJC167513"
- Description: A number of insects, primarily Lepidoptera, cause damage to citrus in South Africa. A major limitation to the management and control of these pests is their correct identification. The aim of this study was to develop a database of gene sequences to aid in the identification of these Lepidoptera. Multiple specimens of 12 species were sequenced for the ~650 bp of the cytochrome oxidase I gene. These sequence data were supplemented and validated using sequences available in public databases. Results showed that each species could be unambiguously identified, and neighbour-joining analysis based on K2P distances formed highly supported, distinct clusters for each species, i.e. the maximum intraspecific genetic distance was less than that of the minimum interspecific genetic distances. Thus, this data set provides a molecular means to successfully identify the most important Lepidoptera associated with citrus in South Africa.
- Full Text:
- Date Issued: 2015
Successful identification of the final instar nymph of Quintilia carinata (Thunberg)(Hemiptera: Cicadidae) by DNA extraction from the exuvium
- Bouwer, Nicolette, Midgley, John M, Timm, Alicia E, Villet, Martin H
- Authors: Bouwer, Nicolette , Midgley, John M , Timm, Alicia E , Villet, Martin H
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442347 , vital:73977 , https://doi.org/10.1080/00222933.2013.836759
- Description: Despite being taxonomically and phylogenetically informative, the morphology of the immature stages of cicadas has received comparatively superficial attention. One reason for this is the difficulty of positively identifying immature stages, particularly as these stages are fossorial. We present a method for identifying cicada exuviae using DNA sequence data and describe a set of characters and character states for the final instar nymph of Quintilia carinata (Thunberg). The identification of immature stages using molecular methods will increase our knowledge of African cicadas, allowing for the initiation of future phylogenetic and ecological comparisons.
- Full Text:
- Date Issued: 2014
- Authors: Bouwer, Nicolette , Midgley, John M , Timm, Alicia E , Villet, Martin H
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442347 , vital:73977 , https://doi.org/10.1080/00222933.2013.836759
- Description: Despite being taxonomically and phylogenetically informative, the morphology of the immature stages of cicadas has received comparatively superficial attention. One reason for this is the difficulty of positively identifying immature stages, particularly as these stages are fossorial. We present a method for identifying cicada exuviae using DNA sequence data and describe a set of characters and character states for the final instar nymph of Quintilia carinata (Thunberg). The identification of immature stages using molecular methods will increase our knowledge of African cicadas, allowing for the initiation of future phylogenetic and ecological comparisons.
- Full Text:
- Date Issued: 2014
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