- Title
- Pharmacological investigations of Kedrostis africana (L.) Cogn. and Vernonia mespilifolia Less. used in folk medicine in the Eastern Cape, South Africa
- Creator
- Unuofin, Jeremiah Oshiomame
- Subject
- Medicinal plants -- South Africa -- Eastern Cape
- Date Issued
- 2017
- Date
- 2017
- Type
- Thesis
- Type
- Doctoral
- Type
- PhD
- Identifier
- http://hdl.handle.net/10353/9231
- Identifier
- vital:34307
- Description
- The prevalence of obesity and its co-morbidity is increasing in South Africa. High calorie diet, sedentary lifestyle and the cultural belief that being fat or obese signifies beauty, wellness and wealth are major causative factors. This study was undertaken to scientifically validate two major medicinal plants used traditionally in the Raymond Mhlaba Municipality for the mespilifolia Less. were used singly and in combination. According to ethnobotanical studies,these two plants are regarded as wild plants and are only used for medicinal purposes. There has been a dearth of scientific reports on the two plants and to the best of our knowledge, this study is the first to investigate the nutritional, antioxidant, antimicrobial, safety and anti-obesity potentials of the two plants and their combination. This study revealed that both plants are rich in nutrients. K. africana had greater ash (16.28 percent), crude fat (1.12 percent), Ca (2505 mg/100g), Mg (485 mg/100g) and Fe (89.95 mg/100g) while V. mespilifolia showed higher crude fibre (29.24 percent), crude protein (10.75 percent), P (400 mg/100g), Na (570 mg/100g), Cu (1.55 mg/100g) and Mn (4.70 mg/100g). K. africana and V. mespilifolia both contributed 223.37 Kcal/kg and 237.37 Kcal/kg of energy respectively. The polyphenolic evaluation of the acetone, aqueous and ethanol extracts of the plants revealed that the acetone extract of the combination of both plants had higher total phenol (144.59 mgGAE/g) and flavonoid (803.93 mgQE/g) contents, the ethanol extract of the combined plant also had the highest tannin contents (1.25 mgTAE/g), while the acetone extracts of K. Africana had the highest proanthocyanidin content (585 mgCE/g). The antioxidant assays revealed that the ethanol extract for V. mespilifolia had higher scavenging potentials of ABTS, DPPH and vinitric oxide radicals while the aqueous extract of the combination of both had higher scavenging potential for hydrogen peroxide radicals. Evaluation of antimicrobial potential of the extracts using the minimial inhibitory concentration (MIC) assay against 6 bacteria (Actinomyces odontolyticus, Lactobacillus sakei, Staphylococcus aureus, Enterobacter cloacae, Pseudomonas aeruginosa and Bacteriodes thetaiotomicron) and 4 fungi (Candida albicans, Microsporium gypsum, Penicillium chrysogenum and Trichophyton tonsurans) revealed that the plants possess antimicrobial activity. The MIC of the extracts against the tested bacterial strains ranged from 2.5 mg/mL to 5 mg/mL acetone and ethanol extracts of V. mespilifolia and the combined plants as well as the ethanol extracts of K. africana. However, no activity was observed for the acetone extract of K. africana and aqueous extracts of V. mespilifolia and the combination of both plants. Only three fungal strains (Candida albicans, Microsporium gypsum and Penicillium chrysogenum) were susceptible to the organic extracts with an MIC 0.3125 mg/mL to 5 mg/mL while the aqueous extracts showed no activity against all the fungal strains. None of the plant extracts showed any activity against Trichophyton tonsurans. The brine shrimp toxicity test revealed that all the three extracts of V. mespilifolia, aqueous and ethanol extracts of K. africana and aqueous and acetone extracts of the combination of both plants were toxic in relative to Meyer’s index and other indices of toxicity. The cytotoxic effect of the aqueous and ethanol extracts of V. mespilifolia, K. africana and their combination were also evaluated using HeLa cells. From this study, all the extracts tested had IC50 values were greater than 20 μg/mL which connotes that they are not toxic. According to the American National Cancer Institute, crude plant extracts are considered cytotoxic in an in vitro assay when concentrations 20 μg/mL and below produce 50% inhibition of tumor cells, after an exposure time of 48 hours. In vivo acute evaluation of single oral administration of 2000 and 5000/kg body weight did not produce mortality or significant behavioral changes during 14 days observation. In addition, the sub-acute administration of the aqueous extract at 200, 400 and 600 mg/kg/bwt/ day over a period of 28 days revealed no mortality or morbidity. The weekly body and organ weight of the rats were not significantly different from those of the controls and extract treated rats. The aqueous extracts at all doses did not show any significant (p > 0.05) effect on biomarkers of liver and renal damage. Haematological evaluation revealed that oral administration of aqueous extracts of K. africana, V. mespilifolia and the combination of both plants did not induce anaemia or leucocytosis in the animals. Furthermore, histopathological evaluation of the internal organs revealed no detectable inflammation at the the doses administered over a period of 28 days. These results demonstrated that the aqueous extracts of K. africana, V. mespilifolia and the combination of both plants was potentially safe for consumption orally even in chronic administration. Enzyme based in-vitro antiobesity evaluation of the aqueous and ethanolic extracts of K. africana, V. mespilifolia and their combination revealed that the ethanol extracts of both plants and their combination exhibited moderate inhibitory activities against α-amylase, α- glucosidase and pancreatic lipase. However, the standards used for the various inhibition assays exhibited much higher inhibitory actions when compared to any of the extracts. This suggests that the mechanism by which these two plants and their combination exert anti-obesity effects are probably not by inhibition of key enzymes of carbohydrate and fat metabolism.
- Format
- 226 leaves
- Format
- Publisher
- University of Fort Hare
- Publisher
- Faculty of Science and Agriculture
- Language
- English
- Rights
- University of Fort Hare
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