The development of biological tools to aid in the genetic investigation of the black (Diceros bicornis) and white (Ceratotherium simum) rhinoceros mitochondrial genomes
- Authors: Parsons, Michelle
- Date: 2015
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/56059 , vital:26769
- Description: The black (Diceros bicornis) and white (Ceratotherium simum) rhinoceros are found in South Africa. A decline in the populations of these species has resulted due to human activities such as habitat fragmentation and poaching. This has contributed to the loss of genetic diversity amongst the black and white rhinoceros. Conservation and anti-poaching efforts are needed to help maintain genetic diversity. These efforts could be improved through the development of non-invasive techniques to examine DNA from threatened animals. The aim of this research was to develop a molecular technique which would allow for the identification of the black and white rhinoceros and to develop a molecular technique which would allow for intraspecies genetic variation to be examined. DNA extractions were performed on matched faecal and tissue samples that were collected from two regions in South Africa. Polymerase chain reaction (PCR) primer sets were designed to investigate several regions of the rhinoceros mitochondrial genome. PCR optimisation was completed for the target regions. Sequencing was conducted on all final PCR products. The cytochrome c oxidase subunit 1 (COIi) gene allowed for the rhinoceros family to be identified. This region was digested with the HindIII restriction enzyme, which allowed for the specific identification of either the black or white rhinoceros. A subsequent region of the cytochrome c oxidase subunit 1 (COIii) as well as the D-loop, hypervariable regions (HV1 and HV2), cytochrome b (cytb) and 16s rRNA regions were investigated. These regions displayed potential for establishing geographic origin for black rhinoceros samples, whereas the D-loop and HV2 show potential for the white rhinoceros. The white rhinoceros displayed sequence variation in the HV2 and cytb region, while variation was observed in the COIi and HV1 for the black rhinoceros. All investigated target regions allowed for the rhinoceros family to be identified. The COI (COIi and COIii), HV2 and cytb regions allowed for the subspecies of rhinoceros to be identified, however the D-loop was not able to identify the white rhinoceros species. The 16s rRNA and HV1 regions allowed for the correct subspecies of rhinoceros to be identified, however as the primers were only compatible for the black rhinoceros therefore a subsequent investigation is required for the white rhinoceros. The establishment of this novel PCR based technique to identify white and black rhinoceros will allow for efficient species identification in wildlife forensic cases. A biological method was established to study intraspecies variation for the white and black rhinoceros; however the investigated target regions did not yield sufficient genetic variation. The core techniques developed in this study will be valuable for future studies that wish to investigate genetic variation in mammal species.
- Full Text:
- Date Issued: 2015
- Authors: Parsons, Michelle
- Date: 2015
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/56059 , vital:26769
- Description: The black (Diceros bicornis) and white (Ceratotherium simum) rhinoceros are found in South Africa. A decline in the populations of these species has resulted due to human activities such as habitat fragmentation and poaching. This has contributed to the loss of genetic diversity amongst the black and white rhinoceros. Conservation and anti-poaching efforts are needed to help maintain genetic diversity. These efforts could be improved through the development of non-invasive techniques to examine DNA from threatened animals. The aim of this research was to develop a molecular technique which would allow for the identification of the black and white rhinoceros and to develop a molecular technique which would allow for intraspecies genetic variation to be examined. DNA extractions were performed on matched faecal and tissue samples that were collected from two regions in South Africa. Polymerase chain reaction (PCR) primer sets were designed to investigate several regions of the rhinoceros mitochondrial genome. PCR optimisation was completed for the target regions. Sequencing was conducted on all final PCR products. The cytochrome c oxidase subunit 1 (COIi) gene allowed for the rhinoceros family to be identified. This region was digested with the HindIII restriction enzyme, which allowed for the specific identification of either the black or white rhinoceros. A subsequent region of the cytochrome c oxidase subunit 1 (COIii) as well as the D-loop, hypervariable regions (HV1 and HV2), cytochrome b (cytb) and 16s rRNA regions were investigated. These regions displayed potential for establishing geographic origin for black rhinoceros samples, whereas the D-loop and HV2 show potential for the white rhinoceros. The white rhinoceros displayed sequence variation in the HV2 and cytb region, while variation was observed in the COIi and HV1 for the black rhinoceros. All investigated target regions allowed for the rhinoceros family to be identified. The COI (COIi and COIii), HV2 and cytb regions allowed for the subspecies of rhinoceros to be identified, however the D-loop was not able to identify the white rhinoceros species. The 16s rRNA and HV1 regions allowed for the correct subspecies of rhinoceros to be identified, however as the primers were only compatible for the black rhinoceros therefore a subsequent investigation is required for the white rhinoceros. The establishment of this novel PCR based technique to identify white and black rhinoceros will allow for efficient species identification in wildlife forensic cases. A biological method was established to study intraspecies variation for the white and black rhinoceros; however the investigated target regions did not yield sufficient genetic variation. The core techniques developed in this study will be valuable for future studies that wish to investigate genetic variation in mammal species.
- Full Text:
- Date Issued: 2015
3-Methyl-1-phenyl-4-[(phenyl)(2-phenylhydrazin-1-yl)methylidene]-1H-pyrazol-5(4H)-one
- Identifier: http://vital.seals.ac.za8080/10353/7489 , http://hdl.handle.net/10353/5328 , vital:29192
- Full Text:
- Identifier: http://vital.seals.ac.za8080/10353/7489 , http://hdl.handle.net/10353/5328 , vital:29192
- Full Text:
Fungal remediation of winery and distillery wastewaters using Trametes pubescens MB 89 and the enhanced production of a high-value enzyme therein
- Authors: Strong, Peter James
- Date: 2008
- Subjects: Fungal remediation Distilleries -- Waste disposal Wine and wine making -- Waste disposal Bioremediation Laccase Enzymes -- Biotechnology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3932 , http://hdl.handle.net/10962/d1003991
- Description: In this study white-rot fungi were investigated for their efficiency at distillery wastewater remediation and the production of laccase as a valuable by-product. Distillery wastewaters are high in organic load and low in pH. The presence of phenolic compounds can lead to extremely colour-rich wastewaters and can be toxic to microorganisms. The presence of the inorganic ions may also affect biological treatment. White-rot fungi are unique among eukaryotic or prokaryotic microbes in possessing powerful oxidative enzyme systems that can degrade lignin to carbon dioxide. These ligninolytic enzymes, such as lignin peroxidase, manganese peroxidase and laccase, are capable of degrading a vast range of toxic, recalcitrant environmental pollutants and this makes the white-rot fungi strong candidates for the bioremediation of polluted soils and waters. The laccase enzyme alone has shown remediation potential in wastewaters such as beer production effluent, olive mill wastewater, alcohol distillery wastes, dye-containing wastewaters from the textile industry as well as wastewaters from the paper and pulp industry. It has been shown to be capable of remediating soils and waters polluted with chlorinated phenolic compounds, polyaromatic hydrocarbons, nitrosubstituted compounds and fungicides, herbicides and insecticides.
- Full Text:
- Date Issued: 2008
- Authors: Strong, Peter James
- Date: 2008
- Subjects: Fungal remediation Distilleries -- Waste disposal Wine and wine making -- Waste disposal Bioremediation Laccase Enzymes -- Biotechnology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3932 , http://hdl.handle.net/10962/d1003991
- Description: In this study white-rot fungi were investigated for their efficiency at distillery wastewater remediation and the production of laccase as a valuable by-product. Distillery wastewaters are high in organic load and low in pH. The presence of phenolic compounds can lead to extremely colour-rich wastewaters and can be toxic to microorganisms. The presence of the inorganic ions may also affect biological treatment. White-rot fungi are unique among eukaryotic or prokaryotic microbes in possessing powerful oxidative enzyme systems that can degrade lignin to carbon dioxide. These ligninolytic enzymes, such as lignin peroxidase, manganese peroxidase and laccase, are capable of degrading a vast range of toxic, recalcitrant environmental pollutants and this makes the white-rot fungi strong candidates for the bioremediation of polluted soils and waters. The laccase enzyme alone has shown remediation potential in wastewaters such as beer production effluent, olive mill wastewater, alcohol distillery wastes, dye-containing wastewaters from the textile industry as well as wastewaters from the paper and pulp industry. It has been shown to be capable of remediating soils and waters polluted with chlorinated phenolic compounds, polyaromatic hydrocarbons, nitrosubstituted compounds and fungicides, herbicides and insecticides.
- Full Text:
- Date Issued: 2008
Synthesis, characterization, and biological studies of pyrazolone Schiff bases and their transition metal complexes
- Authors: Idemudia, Omoruyi Gold
- Date: 2014
- Language: English
- Type: Thesis , Doctoral , PhD (Chemistry)
- Identifier: vital:11340 , http://hdl.handle.net/10353/d1016068
- Description: Some new acylpyrazolone Schiff bases have been synthesized from the condensation reaction of two acylpyrazolone diketone precursors with phenylhydrazine, 2,4-dinitrophenylhydrazine and sulfanilamide. They have been fully characterized by elemental analysis and spectroscopic techniques (IR,1H and 13C NMR, and mass-spectra). The single crystal structure of the benzoyl derivative acylpyrazolone Schiff bases have been obtained and analyzed by X-ray crystallography technique. Solid state X-ray diffraction revealed a keto tautomer Schiff base in solid state. Mn(II), Co(II), Ni(II) and Cu(II) complexes with the Schiff bases have been synthesized and characterized by elemental analysis, IR and UV-VIS spectroscopy, magnetic susceptibility measurements, and thermal studies (TGA and DTG). An octahedral geometry around the transition metal ion, consisting of two bidentate Schiff base ligands bonding through the azometine nitrogen and ketonic oxygen have been proposed based on careful interpretation of available analytical and spectroscopic characterization results. Two water molecules as ligands complete the octahedral geometry in all cases. Using the invitro disc diffusion method for screening synthesized compounds against selected gram positive and gram negative bacterial at 40 mg/mL, and the DPPH free radical scavenging methods at 0.50, 0.25 and 0.13 mg/mL, the synthesized Schiff base and metal complexes showed varying biological activities. 4-benzoyl-3-methyl-1-phenyl-2-pyrazolin-5-one sul29 fanilamide showed more activity generally, exhibiting a broad spectrum activity against all selected bacterial in some cases. Mn(II), Co(II) and Ni(II) complexes of sulfanilamide Schiff base with the acetylpyrazolone derivative 4-acetyl-3-methyl-1-phenyl-2-pyrazolin-5-one sulfanilamide, exhibited a stronger and very good DPPH radical scavenging activity as good as ascorbic acid on comparing, but not with Cu(II). As such they could be important antitumour candidates.
- Full Text:
- Date Issued: 2014
- Authors: Idemudia, Omoruyi Gold
- Date: 2014
- Language: English
- Type: Thesis , Doctoral , PhD (Chemistry)
- Identifier: vital:11340 , http://hdl.handle.net/10353/d1016068
- Description: Some new acylpyrazolone Schiff bases have been synthesized from the condensation reaction of two acylpyrazolone diketone precursors with phenylhydrazine, 2,4-dinitrophenylhydrazine and sulfanilamide. They have been fully characterized by elemental analysis and spectroscopic techniques (IR,1H and 13C NMR, and mass-spectra). The single crystal structure of the benzoyl derivative acylpyrazolone Schiff bases have been obtained and analyzed by X-ray crystallography technique. Solid state X-ray diffraction revealed a keto tautomer Schiff base in solid state. Mn(II), Co(II), Ni(II) and Cu(II) complexes with the Schiff bases have been synthesized and characterized by elemental analysis, IR and UV-VIS spectroscopy, magnetic susceptibility measurements, and thermal studies (TGA and DTG). An octahedral geometry around the transition metal ion, consisting of two bidentate Schiff base ligands bonding through the azometine nitrogen and ketonic oxygen have been proposed based on careful interpretation of available analytical and spectroscopic characterization results. Two water molecules as ligands complete the octahedral geometry in all cases. Using the invitro disc diffusion method for screening synthesized compounds against selected gram positive and gram negative bacterial at 40 mg/mL, and the DPPH free radical scavenging methods at 0.50, 0.25 and 0.13 mg/mL, the synthesized Schiff base and metal complexes showed varying biological activities. 4-benzoyl-3-methyl-1-phenyl-2-pyrazolin-5-one sul29 fanilamide showed more activity generally, exhibiting a broad spectrum activity against all selected bacterial in some cases. Mn(II), Co(II) and Ni(II) complexes of sulfanilamide Schiff base with the acetylpyrazolone derivative 4-acetyl-3-methyl-1-phenyl-2-pyrazolin-5-one sulfanilamide, exhibited a stronger and very good DPPH radical scavenging activity as good as ascorbic acid on comparing, but not with Cu(II). As such they could be important antitumour candidates.
- Full Text:
- Date Issued: 2014
Leonotis leonurus - Lion's ear
- Authors: Skead, C J (Cuthbert John)
- Date: 1960-05-22
- Subjects: Leonotis leonurus -- South Africa -- Photographs , Trees -- South Africa -- Photographs
- Language: English
- Type: mixed material , photographs , landscape photographs
- Identifier: http://hdl.handle.net/10962/122510 , vital:35298
- Description: Caption "Leonotis leonurus beside railway-line east of Berlin, C. P. 22-05-1960."
- Full Text: false
- Date Issued: 1960-05-22
- Authors: Skead, C J (Cuthbert John)
- Date: 1960-05-22
- Subjects: Leonotis leonurus -- South Africa -- Photographs , Trees -- South Africa -- Photographs
- Language: English
- Type: mixed material , photographs , landscape photographs
- Identifier: http://hdl.handle.net/10962/122510 , vital:35298
- Description: Caption "Leonotis leonurus beside railway-line east of Berlin, C. P. 22-05-1960."
- Full Text: false
- Date Issued: 1960-05-22
Inspection of telegraph line
- Authors: McKay, G. , Brownlee, C.
- Date: 1868
- Language: English
- Type: cartographic
- Identifier: http://hdl.handle.net/10962/105127 , vital:32467
- Description: Inspection of telegraph line - G. McKay, East London to Potsdam - C. Brownlee, King Williams Town to Potsdam; [comp. by G. McKay?].Scale not stated. 1 sheet 14½" x 22". Manuscript; may be dated "1868".
- Full Text: false
- Authors: McKay, G. , Brownlee, C.
- Date: 1868
- Language: English
- Type: cartographic
- Identifier: http://hdl.handle.net/10962/105127 , vital:32467
- Description: Inspection of telegraph line - G. McKay, East London to Potsdam - C. Brownlee, King Williams Town to Potsdam; [comp. by G. McKay?].Scale not stated. 1 sheet 14½" x 22". Manuscript; may be dated "1868".
- Full Text: false
The synthesis, empirical and theoretical studies of various (N→B) diarylborinate esters
- Authors: Manana, Pholani Sakhile
- Date: 2021-12
- Subjects: Port Elizabeth (South Africa) , Eastern Cape (South Africa) , South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/53869 , vital:46027
- Description: A series of core-functionalized borinic acids was synthesized from Grignard reagents and tributylborate and reacted with ethanolamine, amino acids, 2-pyridylmethanol as well as 2-(ethyl amino ethanol). Among the borinic acids synthesized were the ones bearing: para-(2, 15, 16, 22, 27)-methyl, (3, 20, 25)-chloro, (4, 23, 26)-fluoro, (5)-methoxy; meta-(7, 17)-methyl, (9)-fluoro; ortho-(6)-methyl, (8)-chloro, (10, 18, 19)-methoxy substituents on their respective aromatic systems. All compounds obtained by condensation of the borinic acids with the aforementioned nitrogen-containing substrates were characterized structurally by means of diffraction studies based on single crystals and found to constitute chelate esters/amides featuring covalent B-O and dative N→B bonds. The chelate compounds researched in this study could be classified into four families and represent the compounds: ethanol amine: (1) 2,2-diphenyl-1,3,2-oxazaborolidines, (2) 2,2-bis(p-tolyl)-1,3,2-oxazaborolidines, (3) 2,2-bis(p-chlorophenyl)-1,3,2-oxazaborolidines, (4) 2,2-bis(p-fluorophenyl)-1,3,2-oxazaborolidines, (5) 2,2-bis(p-methoxyphenyl)-1,3,2-oxazaborolidines, (6) 2,2-bis(o-tolyl)-1,3,2-oxazaborolidines, (7) 2,2-bis(m-tolyl)-1,3,2-oxazaborolidines, (8) 2,2-bis(o-chlorophenyl)-1,3,2-oxazaborolidines, (9) 2,2-bis(m-fluorophenyl)-1,3,2-oxazaborolidines, (10) 2,2-bis(o-methoxyphenyl)-1,3,2-oxazaborolidines. α-amino acids : (14) 4-methyl-2,2-diphenyl-1,3,2-oxazaborolidin-5-ones, (15) 2,2-bis(p-tolyl)-1,3,2-oxazaborolidin-5-ones, (16) 4-methyl-2,2-bis(p-tolyl)-1,3,2-oxazaborolidin-5-ones, (17) 4-isopropyl-2,2-bis(m-tolyl)-1,3,2-oxazaborolidin-5-ones, (18) 4-methyl-2,2-bis(o-methoxyphenyl)-1,3,2- oxazaborolidin-5-ones, (19) 4-acetamidyl-2,2-bis(o-methoxyphenyl)-1,3,2- oxazaborolidin-5-ones, (20) 4-phenylethane-2,2-bis(p-chlorophenyl)-1,3,2 oxazaborolidin-5-ones. 2-pyridylmethanol: (21) 1,3-hydroxypyridyl-2,2-diphenyl 1,3,2-oxazaborolidines, (22) 1,3-hydroxypyridyl-2,2-bis(p-tolyl)-1,3,2-oxazaborolidines, (23) 1,3-hydroxypyridyl-2,2-bis(p-fluorophenyl)-1,3,2-oxazaborolidines 2-(ethyl amino ethanol): (24) 3-ethyl-2,2-diphenyl-1,3,2-oxazaborolidines, (25) 3-ethyl-2,2-bis(p-chlorophenyl)-1,3,2-oxazaborolidines, (26) 3-ethyl-2,2-bis(p-fluorophenyl)-1,3,2-oxazaborolidines, (27) 3-ethyl-2,2-bis(p-tolyl)-1,3,2-oxazaborolidines. Apart from structural studies in the solid state, the compounds were also characterized by means of multinuclear NMR spectroscopy (1H, 11B, 13C) as well as UV/vis and FTIR. The typical 11B resonances were found in the range of (4-10 ppm). A series of characteristic metrical parameters for the compounds in the solid state as well as typical NMR shifts for the chelate target molecules was established that allowed for the determination of the coordination induced shift. The carbon atom bonded to the oxygen atom shifted upfield, while the carbon atom bonded to the nitrogen atom shifted downfield. Factors mentioned in the literature that has an influence on the hydrolytic stability on the heterocyclic borinate motif have been taken into consideration and verified for the systems researched in this study. The substitution at the nitrogen atom with one ethyl group caused the greatest increases in the N→B bond length (27) 1.714 Å, due to the increase in the nitrogen Lewis basicity, making it the least hydrolytically stable, these include compounds (24-26). Furthermore, DFT calculations were performed by employing Becke’s three parameter hybrid exchange functional with Lee–Yang–Parr functional (B3LYP) method using the Gaussian 16 program package (Rev. B01) employing the basis set 6-311++G(d,p), to corroborate and correlate the experimental findings, which gave a general R-squared value of 0.9978 for calculated vs experimental 13C chemical shifts. Thermal properties are recorded for select compounds, indicating the ΔH and the melting points. , Thesis (PhD) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2021
- Full Text:
- Date Issued: 2021-12
- Authors: Manana, Pholani Sakhile
- Date: 2021-12
- Subjects: Port Elizabeth (South Africa) , Eastern Cape (South Africa) , South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/53869 , vital:46027
- Description: A series of core-functionalized borinic acids was synthesized from Grignard reagents and tributylborate and reacted with ethanolamine, amino acids, 2-pyridylmethanol as well as 2-(ethyl amino ethanol). Among the borinic acids synthesized were the ones bearing: para-(2, 15, 16, 22, 27)-methyl, (3, 20, 25)-chloro, (4, 23, 26)-fluoro, (5)-methoxy; meta-(7, 17)-methyl, (9)-fluoro; ortho-(6)-methyl, (8)-chloro, (10, 18, 19)-methoxy substituents on their respective aromatic systems. All compounds obtained by condensation of the borinic acids with the aforementioned nitrogen-containing substrates were characterized structurally by means of diffraction studies based on single crystals and found to constitute chelate esters/amides featuring covalent B-O and dative N→B bonds. The chelate compounds researched in this study could be classified into four families and represent the compounds: ethanol amine: (1) 2,2-diphenyl-1,3,2-oxazaborolidines, (2) 2,2-bis(p-tolyl)-1,3,2-oxazaborolidines, (3) 2,2-bis(p-chlorophenyl)-1,3,2-oxazaborolidines, (4) 2,2-bis(p-fluorophenyl)-1,3,2-oxazaborolidines, (5) 2,2-bis(p-methoxyphenyl)-1,3,2-oxazaborolidines, (6) 2,2-bis(o-tolyl)-1,3,2-oxazaborolidines, (7) 2,2-bis(m-tolyl)-1,3,2-oxazaborolidines, (8) 2,2-bis(o-chlorophenyl)-1,3,2-oxazaborolidines, (9) 2,2-bis(m-fluorophenyl)-1,3,2-oxazaborolidines, (10) 2,2-bis(o-methoxyphenyl)-1,3,2-oxazaborolidines. α-amino acids : (14) 4-methyl-2,2-diphenyl-1,3,2-oxazaborolidin-5-ones, (15) 2,2-bis(p-tolyl)-1,3,2-oxazaborolidin-5-ones, (16) 4-methyl-2,2-bis(p-tolyl)-1,3,2-oxazaborolidin-5-ones, (17) 4-isopropyl-2,2-bis(m-tolyl)-1,3,2-oxazaborolidin-5-ones, (18) 4-methyl-2,2-bis(o-methoxyphenyl)-1,3,2- oxazaborolidin-5-ones, (19) 4-acetamidyl-2,2-bis(o-methoxyphenyl)-1,3,2- oxazaborolidin-5-ones, (20) 4-phenylethane-2,2-bis(p-chlorophenyl)-1,3,2 oxazaborolidin-5-ones. 2-pyridylmethanol: (21) 1,3-hydroxypyridyl-2,2-diphenyl 1,3,2-oxazaborolidines, (22) 1,3-hydroxypyridyl-2,2-bis(p-tolyl)-1,3,2-oxazaborolidines, (23) 1,3-hydroxypyridyl-2,2-bis(p-fluorophenyl)-1,3,2-oxazaborolidines 2-(ethyl amino ethanol): (24) 3-ethyl-2,2-diphenyl-1,3,2-oxazaborolidines, (25) 3-ethyl-2,2-bis(p-chlorophenyl)-1,3,2-oxazaborolidines, (26) 3-ethyl-2,2-bis(p-fluorophenyl)-1,3,2-oxazaborolidines, (27) 3-ethyl-2,2-bis(p-tolyl)-1,3,2-oxazaborolidines. Apart from structural studies in the solid state, the compounds were also characterized by means of multinuclear NMR spectroscopy (1H, 11B, 13C) as well as UV/vis and FTIR. The typical 11B resonances were found in the range of (4-10 ppm). A series of characteristic metrical parameters for the compounds in the solid state as well as typical NMR shifts for the chelate target molecules was established that allowed for the determination of the coordination induced shift. The carbon atom bonded to the oxygen atom shifted upfield, while the carbon atom bonded to the nitrogen atom shifted downfield. Factors mentioned in the literature that has an influence on the hydrolytic stability on the heterocyclic borinate motif have been taken into consideration and verified for the systems researched in this study. The substitution at the nitrogen atom with one ethyl group caused the greatest increases in the N→B bond length (27) 1.714 Å, due to the increase in the nitrogen Lewis basicity, making it the least hydrolytically stable, these include compounds (24-26). Furthermore, DFT calculations were performed by employing Becke’s three parameter hybrid exchange functional with Lee–Yang–Parr functional (B3LYP) method using the Gaussian 16 program package (Rev. B01) employing the basis set 6-311++G(d,p), to corroborate and correlate the experimental findings, which gave a general R-squared value of 0.9978 for calculated vs experimental 13C chemical shifts. Thermal properties are recorded for select compounds, indicating the ΔH and the melting points. , Thesis (PhD) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2021
- Full Text:
- Date Issued: 2021-12
Recovery after fire of veld at NMMU campus.
- Authors: Skead, C J (Cuthbert John)
- Date: 2006-02-22
- Subjects: Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:12942 , http://hdl.handle.net/10962/d1015103
- Description: Recovery after fire of veld at NMMU campus.
- Full Text: false
- Date Issued: 2006-02-22
- Authors: Skead, C J (Cuthbert John)
- Date: 2006-02-22
- Subjects: Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:12942 , http://hdl.handle.net/10962/d1015103
- Description: Recovery after fire of veld at NMMU campus.
- Full Text: false
- Date Issued: 2006-02-22
Recovery after fire of veld at NMMU campus.
- Authors: Skead, C J (Cuthbert John)
- Date: 2006-02-22
- Subjects: Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:12943 , http://hdl.handle.net/10962/d1015104
- Description: Recovery after fire of veld at NMMU campus.
- Full Text: false
- Date Issued: 2006-02-22
- Authors: Skead, C J (Cuthbert John)
- Date: 2006-02-22
- Subjects: Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:12943 , http://hdl.handle.net/10962/d1015104
- Description: Recovery after fire of veld at NMMU campus.
- Full Text: false
- Date Issued: 2006-02-22
Schoenmakerskop after fire in November 2005
- Authors: Skead, C J (Cuthbert John)
- Date: 2006-02-22
- Subjects: Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:12941 , http://hdl.handle.net/10962/d1015102
- Description: Schoenmakerskop after fire in November 2005.
- Full Text: false
- Date Issued: 2006-02-22
- Authors: Skead, C J (Cuthbert John)
- Date: 2006-02-22
- Subjects: Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:12941 , http://hdl.handle.net/10962/d1015102
- Description: Schoenmakerskop after fire in November 2005.
- Full Text: false
- Date Issued: 2006-02-22
Bushveld behind NMMU on North side of road leading towards Admin Block
- Authors: Skead, C J (Cuthbert John)
- Date: 2005-12-22
- Subjects: Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:12940 , http://hdl.handle.net/10962/d1015101
- Description: Bushveld behind NMMU on North side of road leading towards Admin Block.
- Full Text: false
- Date Issued: 2005-12-22
- Authors: Skead, C J (Cuthbert John)
- Date: 2005-12-22
- Subjects: Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:12940 , http://hdl.handle.net/10962/d1015101
- Description: Bushveld behind NMMU on North side of road leading towards Admin Block.
- Full Text: false
- Date Issued: 2005-12-22
Bushveld behind NMMU on South side of road
- Authors: Skead, C J (Cuthbert John)
- Date: 2005-12-22
- Subjects: Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:12938 , http://hdl.handle.net/10962/d1015098
- Description: Bushveld behind NMMU on South side of road.
- Full Text: false
- Date Issued: 2005-12-22
- Authors: Skead, C J (Cuthbert John)
- Date: 2005-12-22
- Subjects: Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:12938 , http://hdl.handle.net/10962/d1015098
- Description: Bushveld behind NMMU on South side of road.
- Full Text: false
- Date Issued: 2005-12-22
Bushveld behind NMMU on South side of road
- Authors: Skead, C J (Cuthbert John)
- Date: 2005-12-22
- Subjects: Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:12939 , http://hdl.handle.net/10962/d1015099
- Description: Bushveld behind NMMU on South side of road.
- Full Text: false
- Date Issued: 2005-12-22
- Authors: Skead, C J (Cuthbert John)
- Date: 2005-12-22
- Subjects: Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:12939 , http://hdl.handle.net/10962/d1015099
- Description: Bushveld behind NMMU on South side of road.
- Full Text: false
- Date Issued: 2005-12-22
NMM University ground just north of Chelsea Point
- Authors: Skead, C J (Cuthbert John)
- Date: 2005-12-22
- Subjects: Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:12936 , http://hdl.handle.net/10962/d1015095
- Description: NMM University ground just north of Chelsea Point.
- Full Text: false
- Date Issued: 2005-12-22
- Authors: Skead, C J (Cuthbert John)
- Date: 2005-12-22
- Subjects: Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:12936 , http://hdl.handle.net/10962/d1015095
- Description: NMM University ground just north of Chelsea Point.
- Full Text: false
- Date Issued: 2005-12-22
NMM University ground just north of Chelsea Point
- Authors: Skead, C J (Cuthbert John)
- Date: 2005-12-22
- Subjects: Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:12937 , http://hdl.handle.net/10962/d1015097
- Description: NMM University ground just north of Chelsea Point - Flat stony ground.
- Full Text: false
- Date Issued: 2005-12-22
- Authors: Skead, C J (Cuthbert John)
- Date: 2005-12-22
- Subjects: Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:12937 , http://hdl.handle.net/10962/d1015097
- Description: NMM University ground just north of Chelsea Point - Flat stony ground.
- Full Text: false
- Date Issued: 2005-12-22
Schoenmakerskop after fire in November 2005
- Authors: Skead, C J (Cuthbert John)
- Date: 2005-12-22
- Subjects: Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:12935 , http://hdl.handle.net/10962/d1015094
- Description: Schoenmakerskop after fire in November 2005.
- Full Text: false
- Date Issued: 2005-12-22
- Authors: Skead, C J (Cuthbert John)
- Date: 2005-12-22
- Subjects: Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:12935 , http://hdl.handle.net/10962/d1015094
- Description: Schoenmakerskop after fire in November 2005.
- Full Text: false
- Date Issued: 2005-12-22
Kruger Gardens, Port Elizabeth (Summerstrand)
- Authors: Skead, C J (Cuthbert John)
- Date: 1994-02-22
- Subjects: Cyrtanthus sanguineus -- South Africa -- Eastern Cape , Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:13011 , http://hdl.handle.net/10962/d1016644
- Description: 1 bowl of Cyrtanthus sanguineus. 42 in flower.
- Full Text: false
- Date Issued: 1994-02-22
- Authors: Skead, C J (Cuthbert John)
- Date: 1994-02-22
- Subjects: Cyrtanthus sanguineus -- South Africa -- Eastern Cape , Plants -- South Africa -- Eastern Cape
- Type: still image
- Identifier: vital:13011 , http://hdl.handle.net/10962/d1016644
- Description: 1 bowl of Cyrtanthus sanguineus. 42 in flower.
- Full Text: false
- Date Issued: 1994-02-22
Speech by Chief Mwanjawanthu
- Chief Mwanjawanthu, performer not specified, Composer not specified, Tracey, Hugh
- Authors: Chief Mwanjawanthu , performer not specified , Composer not specified , Tracey, Hugh
- Date: 1949
- Subjects: Folk Music , Field recordings , Africa, Sub-Saharan , Africa Zambia Lusaka f-za
- Language: Nsenga
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/380191 , vital:67428 , International Library of African Music, Rhodes University, Makhanda, South Africa , Hugh Tracey Commercial Records, Rhodes University, Makhanda, South Africa , AC0549-C4V22
- Description: Indigenous music
- Full Text: false
- Date Issued: 1949
- Authors: Chief Mwanjawanthu , performer not specified , Composer not specified , Tracey, Hugh
- Date: 1949
- Subjects: Folk Music , Field recordings , Africa, Sub-Saharan , Africa Zambia Lusaka f-za
- Language: Nsenga
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/380191 , vital:67428 , International Library of African Music, Rhodes University, Makhanda, South Africa , Hugh Tracey Commercial Records, Rhodes University, Makhanda, South Africa , AC0549-C4V22
- Description: Indigenous music
- Full Text: false
- Date Issued: 1949
Synthesis, characterization and biological activity of some Dithiourea Derivatives:
- Odame, Felix, Hosten, Eric, Krause, Jason, Isaacs, Michelle, Hoppe, Heinrich C, Khanye, Setshaba D, Sayed, Yasien, Frost, Carminita, Lobb, Kevin A, Tshentu, Zenixole
- Authors: Odame, Felix , Hosten, Eric , Krause, Jason , Isaacs, Michelle , Hoppe, Heinrich C , Khanye, Setshaba D , Sayed, Yasien , Frost, Carminita , Lobb, Kevin A , Tshentu, Zenixole
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/163046 , vital:41007 , DOI: 10.17344/acsi.2019.5689
- Description: Novel dithiourea derivatives have been designed as HIV-1 protease inhibitors using Autodock 4.2, synthesized and characterized by spectroscopic methods and microanalysis.
- Full Text:
- Date Issued: 2020
- Authors: Odame, Felix , Hosten, Eric , Krause, Jason , Isaacs, Michelle , Hoppe, Heinrich C , Khanye, Setshaba D , Sayed, Yasien , Frost, Carminita , Lobb, Kevin A , Tshentu, Zenixole
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/163046 , vital:41007 , DOI: 10.17344/acsi.2019.5689
- Description: Novel dithiourea derivatives have been designed as HIV-1 protease inhibitors using Autodock 4.2, synthesized and characterized by spectroscopic methods and microanalysis.
- Full Text:
- Date Issued: 2020
Network analysis of trophic linkages in two sub-tropical estuaries along the South-East coast of South Africa
- Authors: Vosloo, Mathys Christiaan
- Date: 2012
- Subjects: Estuaries -- South Africa -- Eastern Cape , Estuarine ecology , Network analysis (Planning)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10708 , http://hdl.handle.net/10948/d1010966 , Estuaries -- South Africa -- Eastern Cape , Estuarine ecology , Network analysis (Planning)
- Description: Estuaries are some of the most productive yet threatened ecosystems in the world. Despite their importance they face significant threats through changes to river flow, eutrophication, rapid population growth long the caost and harvesting of natural resources. A number of international studies have been conducted investigating the structure and functioning of an array of ecosystems using ecological network analysis. Energy flow networks have been contsructed for coastal, lagoonal, intertidial and, most notably, permantently open estuaries. Despite the valualble insights contributed by these and other studies, a lack of information on the majority of estuarine ecosystems exists.
- Full Text:
- Date Issued: 2012
- Authors: Vosloo, Mathys Christiaan
- Date: 2012
- Subjects: Estuaries -- South Africa -- Eastern Cape , Estuarine ecology , Network analysis (Planning)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10708 , http://hdl.handle.net/10948/d1010966 , Estuaries -- South Africa -- Eastern Cape , Estuarine ecology , Network analysis (Planning)
- Description: Estuaries are some of the most productive yet threatened ecosystems in the world. Despite their importance they face significant threats through changes to river flow, eutrophication, rapid population growth long the caost and harvesting of natural resources. A number of international studies have been conducted investigating the structure and functioning of an array of ecosystems using ecological network analysis. Energy flow networks have been contsructed for coastal, lagoonal, intertidial and, most notably, permantently open estuaries. Despite the valualble insights contributed by these and other studies, a lack of information on the majority of estuarine ecosystems exists.
- Full Text:
- Date Issued: 2012