New platinum and palladium complexes: their anticancer application
- Authors: Louw, Marissa
- Date: 2010
- Subjects: Complex compounds -- Synthesis , Ligands (Biochemistry) , Antineoplastic antibiotics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10424 , http://hdl.handle.net/10948/d1016218
- Description: Novel non-leaving groups were employed in this dissertation to synthesize platinum complexes which can assist in the understanding or improvement of anticancer action. Emphasis was placed on (NS)-chelate and (NN)-chelate platinum complexes. Bidentate (NS)-donor ligands were used as non-leaving ligands in the synthesis of platinum(II) complexes with iodo, chloro, bromo and oxalato groups as leaving groups. These complexes were synthesized and studied since many questions regarding the interaction of sulfur-donors and platinum still exist. These relate to thermodynamic and kinetic factors and their influence on anticancer action. In this dissertation the properties of novel platinum(II) complexes of a bidentate ligand having an aromatic nitrogen-donor atom in combination with a thioethereal sulfur atom capable of forming a five-membered ring with platinum(II) were studied. The general structure of the (NS)-ligands used was 2-((alkylthio)methyl)pyridine. Alkyl groups used were methyl, ethyl, propyl, benzyl and phenyl. Amine complexes of platinum have been studied extensively in the past. However, attention was given to novel aspects of substituted pyridine and imidazole ligands and their corresponding complexes. Amongst these are 2-(2-methylaminoethyl)pyridine, 1-methyl-2-methylaminoethylimidazole and 1-methyl-2-methylaminobenzylimidazole. The leaving groups included chloro, bromo and oxalato. Mononitroplatinum(IV) complexes were prepared using novel synthetic methods. Selected platinum(II) amine complexes were used as starting materials for this synthesis. Some of these compounds exhibit promising anticancer behaviour. (Trans-(R,R)-1,2-diaminocyclohexane)(oxalato)(mononitrochloro)platinum(IV) is a particularly good anticancer agent and has been patented internationally. All these complexes were characterized using mass spectrometry, chromatography, thermogravimetric analysis, kinetic aspects such as ligand exchange rates and finally their anticancer action against three different cancer cell lines was evaluated via cytotoxicity assays. Some of the compounds exhibited particularly good anticancer potential.
- Full Text:
- Date Issued: 2010
- Authors: Louw, Marissa
- Date: 2010
- Subjects: Complex compounds -- Synthesis , Ligands (Biochemistry) , Antineoplastic antibiotics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10424 , http://hdl.handle.net/10948/d1016218
- Description: Novel non-leaving groups were employed in this dissertation to synthesize platinum complexes which can assist in the understanding or improvement of anticancer action. Emphasis was placed on (NS)-chelate and (NN)-chelate platinum complexes. Bidentate (NS)-donor ligands were used as non-leaving ligands in the synthesis of platinum(II) complexes with iodo, chloro, bromo and oxalato groups as leaving groups. These complexes were synthesized and studied since many questions regarding the interaction of sulfur-donors and platinum still exist. These relate to thermodynamic and kinetic factors and their influence on anticancer action. In this dissertation the properties of novel platinum(II) complexes of a bidentate ligand having an aromatic nitrogen-donor atom in combination with a thioethereal sulfur atom capable of forming a five-membered ring with platinum(II) were studied. The general structure of the (NS)-ligands used was 2-((alkylthio)methyl)pyridine. Alkyl groups used were methyl, ethyl, propyl, benzyl and phenyl. Amine complexes of platinum have been studied extensively in the past. However, attention was given to novel aspects of substituted pyridine and imidazole ligands and their corresponding complexes. Amongst these are 2-(2-methylaminoethyl)pyridine, 1-methyl-2-methylaminoethylimidazole and 1-methyl-2-methylaminobenzylimidazole. The leaving groups included chloro, bromo and oxalato. Mononitroplatinum(IV) complexes were prepared using novel synthetic methods. Selected platinum(II) amine complexes were used as starting materials for this synthesis. Some of these compounds exhibit promising anticancer behaviour. (Trans-(R,R)-1,2-diaminocyclohexane)(oxalato)(mononitrochloro)platinum(IV) is a particularly good anticancer agent and has been patented internationally. All these complexes were characterized using mass spectrometry, chromatography, thermogravimetric analysis, kinetic aspects such as ligand exchange rates and finally their anticancer action against three different cancer cell lines was evaluated via cytotoxicity assays. Some of the compounds exhibited particularly good anticancer potential.
- Full Text:
- Date Issued: 2010
Photophysical and photoelectrochemical properties of water soluble metallophthalocyanines
- Authors: Masilela, Nkosiphile
- Date: 2010
- Subjects: Phthalocyanines , Electrochemistry , Photoelectrochemistry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4330 , http://hdl.handle.net/10962/d1004991 , Phthalocyanines , Electrochemistry , Photoelectrochemistry
- Description: This work presents the synthesis, characterization, photophysicochemical and photoelectrochemical properties of anionic octa-caboxylated (MOCPcs), tetra-sulfonated (MTSPcs) and quaternized cationic (Q(T-2-Py)MPcs) water soluble aluminium, gallium, silicon, titanium and zinc phthalocynines. The peripherally tetra-substituted cationic (Q(T-2-Py)MPcs) and anionic (MTSPcs) were found to be aggregated in aqueous media, yet the octa-carboxylated (MOCPcs) counterparts were monomeric in solution. Cremophor EL (CEL) was used as a disaggregating agent, all the aggregated complexes disaggregated partially or completely in the presence of CEL. The photophysicochemical properties of aggregated complexes were investigated both in the presence of CEL and in aqueous media of pH 11 alone. Low triplet, singlet oxygen and fluorescence quantum yield were obtained in aqueous media (especially for the aggregated complexes) but a high improvement was achieved upon addition of CEL. The gallium complexes ((OH)GaOCPc and (OH)GaTSPc) showed good photophysicochemical properties with higher triplet and singlet oxygen quantum yields. For photoelectrochemistry the (MPcs) dyes were adsorbed to nanoporous ZnO, electrodeposited in the presence of eosin Y as structure directing agent (SDA) on FTO substrates by refluxing or soaking the films in a solution containing the dye of interest such that a full surface coverage was achieved. Quaternized cationic (Q(T-2-Py)MPc) and tetrasulfonated (MTSPcs) phthalocyanines formed strong aggregates when deposited on the surface of FTO/ZnO substrate leading. High external (IPCE) and internal (APCE) quantum efficiencies of up to 50.6% and 96.7% were achieved for the OTiOCPc complex. There was a lower overall cell efficiency for quaternized and tetrasulfonated metallophthalocyanines because of the strong aggregates when they were on the surface of the electrodes. Among the studied materials, OTiOCPc gave the highest overall cell efficiency of phthalocyanine electrodeposited on ZnO of so far = 0.48%.
- Full Text:
- Date Issued: 2010
- Authors: Masilela, Nkosiphile
- Date: 2010
- Subjects: Phthalocyanines , Electrochemistry , Photoelectrochemistry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4330 , http://hdl.handle.net/10962/d1004991 , Phthalocyanines , Electrochemistry , Photoelectrochemistry
- Description: This work presents the synthesis, characterization, photophysicochemical and photoelectrochemical properties of anionic octa-caboxylated (MOCPcs), tetra-sulfonated (MTSPcs) and quaternized cationic (Q(T-2-Py)MPcs) water soluble aluminium, gallium, silicon, titanium and zinc phthalocynines. The peripherally tetra-substituted cationic (Q(T-2-Py)MPcs) and anionic (MTSPcs) were found to be aggregated in aqueous media, yet the octa-carboxylated (MOCPcs) counterparts were monomeric in solution. Cremophor EL (CEL) was used as a disaggregating agent, all the aggregated complexes disaggregated partially or completely in the presence of CEL. The photophysicochemical properties of aggregated complexes were investigated both in the presence of CEL and in aqueous media of pH 11 alone. Low triplet, singlet oxygen and fluorescence quantum yield were obtained in aqueous media (especially for the aggregated complexes) but a high improvement was achieved upon addition of CEL. The gallium complexes ((OH)GaOCPc and (OH)GaTSPc) showed good photophysicochemical properties with higher triplet and singlet oxygen quantum yields. For photoelectrochemistry the (MPcs) dyes were adsorbed to nanoporous ZnO, electrodeposited in the presence of eosin Y as structure directing agent (SDA) on FTO substrates by refluxing or soaking the films in a solution containing the dye of interest such that a full surface coverage was achieved. Quaternized cationic (Q(T-2-Py)MPc) and tetrasulfonated (MTSPcs) phthalocyanines formed strong aggregates when deposited on the surface of FTO/ZnO substrate leading. High external (IPCE) and internal (APCE) quantum efficiencies of up to 50.6% and 96.7% were achieved for the OTiOCPc complex. There was a lower overall cell efficiency for quaternized and tetrasulfonated metallophthalocyanines because of the strong aggregates when they were on the surface of the electrodes. Among the studied materials, OTiOCPc gave the highest overall cell efficiency of phthalocyanine electrodeposited on ZnO of so far = 0.48%.
- Full Text:
- Date Issued: 2010
Photophysical studies of zinc and indium tetraaminophthalocyanines in the presence of CdTe quantum dots
- Authors: Britton, Jonathan
- Date: 2010
- Subjects: Indium , Zinc , Quantum dots , Phthalocyanines , Photochemotherapy , Nonlinear optics , Nanocrystals
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4332 , http://hdl.handle.net/10962/d1004993 , Indium , Zinc , Quantum dots , Phthalocyanines , Photochemotherapy , Nonlinear optics , Nanocrystals
- Description: CdTe QDs capped with mercaptopropionic acid (MPA) and thioglycolic acid (TGA) were covalently linked to zinc and indium tetraaminophthalocyanines (TAPcs) using N-ethyl-N(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxy succinimide (NHS) as the coupling agents. The results presented give evidence in favour of formation of an amide bond between the MTAPc and CdTe QDs. Both the linked ZnTAPc–QD complexes and the mixture of QDs and ZnTAPc (without chemical linking) showed Förster resonance energy transfer (FRET), though the linked showed less FRET, whereas the QD interactions with InTAPc yielded no evidence of FRET. Both MTAPcs quenched the QDs emission, with quenching constants of the order of 103–104M−1, binding constants of the order of 108-1010M-1 and the number of binding sites for the MTAPc upon the QD being 2. High energy transfer efficiencies were obtained (in some cases as high as 93%), due to the low donor to acceptor distances. Lastly, both MTAPc were shown to be poor optical limiters because their imaginary third-order susceptibility (Im[χ(3)]) was of the order of 10-17-10-16 (optimal range is 10-9-10-11), the hyperpolarizability (γ) of the order of 10-37-10-36 (optimal range is 10-29-10-34) and the k values were above one but below ten.
- Full Text:
- Date Issued: 2010
- Authors: Britton, Jonathan
- Date: 2010
- Subjects: Indium , Zinc , Quantum dots , Phthalocyanines , Photochemotherapy , Nonlinear optics , Nanocrystals
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4332 , http://hdl.handle.net/10962/d1004993 , Indium , Zinc , Quantum dots , Phthalocyanines , Photochemotherapy , Nonlinear optics , Nanocrystals
- Description: CdTe QDs capped with mercaptopropionic acid (MPA) and thioglycolic acid (TGA) were covalently linked to zinc and indium tetraaminophthalocyanines (TAPcs) using N-ethyl-N(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxy succinimide (NHS) as the coupling agents. The results presented give evidence in favour of formation of an amide bond between the MTAPc and CdTe QDs. Both the linked ZnTAPc–QD complexes and the mixture of QDs and ZnTAPc (without chemical linking) showed Förster resonance energy transfer (FRET), though the linked showed less FRET, whereas the QD interactions with InTAPc yielded no evidence of FRET. Both MTAPcs quenched the QDs emission, with quenching constants of the order of 103–104M−1, binding constants of the order of 108-1010M-1 and the number of binding sites for the MTAPc upon the QD being 2. High energy transfer efficiencies were obtained (in some cases as high as 93%), due to the low donor to acceptor distances. Lastly, both MTAPc were shown to be poor optical limiters because their imaginary third-order susceptibility (Im[χ(3)]) was of the order of 10-17-10-16 (optimal range is 10-9-10-11), the hyperpolarizability (γ) of the order of 10-37-10-36 (optimal range is 10-29-10-34) and the k values were above one but below ten.
- Full Text:
- Date Issued: 2010
Reactivity of Rhenium (iii) and Rhenium (V) with multidentate NN-and no-donor ligands
- Yumata, Nonzaliseko Christine
- Authors: Yumata, Nonzaliseko Christine
- Date: 2010
- Subjects: Rhenium , Ligands
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10373 , http://hdl.handle.net/10948/1085 , Rhenium , Ligands
- Description: The reaction of the potentially tridentate Schiff-base chelate Hhaep [Haep = N’-(1- (2-hydroxyphenyl)ethylidene)benzohydrazide] with [ReCl3(benzil)(PPh3)] and trans-[ReOCl3(PPh3)2] produced the neutral oxorhenium(V) complexes cis- [ReOCl2(mep)] [Hmep = 2-(1-iminoethyl)phenol] and cis-[ReOCI2(meb)(PPh3)] [Hmeb = N’-(propan-2-ylidene)benzohydrazide] in ethanol and acetone respectively. In both reactions the Hhaep molecule cleaves to give different coordinated bidentate NO-donor chelates coordinated to the rhenium(V) centers. The X-ray studies reveal that mep is present as a bidentate, monoanionic Schiffbase coordinating through the neutral imino nitrogen and the deprotonated phenolate oxygen in cis-[ReOCl2(mep)]. The bond distances and angles in cis- [ReOCI2(meb)(PPh3)] confirm that meb coordinates to the metal in the enolate form. The distorted octahedral complex fac-[ReCl3(dpa)(PPh3)] was prepared by the reaction of trans-[ReCl3(MeCN)(PPh3)2] with a twofold molar excess of dpa in acetonitrile under a nitrogen atmosphere. The compound dpa.HCl.2H2O was obtained as a by-product in the reaction of dpa with trans-[ReCI3(MeCN)(PPh3)2] in acetonitrile. The reaction of trans-[ReCl3(MeCN)(PPh3)2] with a twofold molar excess of 6- amino-3-methyl-1-phenyl-4-azahept-2-ene-1-one (Hamp) in acetonitrile led to the isolation of cis-[ReCl2(bat)(PPh3)2]. On complexation to the metal center Hamp decomposed to give the coordinated benzoylacetone (bat). Bat is present as a monoanionic bidentate chelate. The complexes [ReVOCI(had)] and [ReIVCl(had)(PPh3)](ReO4) were prepared from the reaction of trans-[ReCl3(MeCN)(PPh3)2] with N,N-bis((2-hydroxybenzyl)-2- aminoethyl)dimethylamine (H2had) in ethanol under various reaction conditions. The treatment of [ReCl3(benzil)(PPh3)] with 2-[((2- pyridinylmethyl)amino)methyl]phenol (Hham) in a 2:1 molar ratio in acetonitrile led to the isolation of the hydrogen-bonded dimer [ReOCl2(ham)]2. The distorted octahedral complex [ReOCl(hap)] [H2hap = N,N-bis(2- hydroxybenzyl)aminomethylpyridine] was prepared from the reaction of trans- [ReCl3(MeCN)(PPh3)2] with a twofold molar excess of H2hap in acetonitrile. The X-ray crystal structure analysis shows that the chloride is coordinated trans to the tripodal tertiary amino nitrogen, with a phenolate oxygen trans to the oxo oxygen.
- Full Text:
- Date Issued: 2010
- Authors: Yumata, Nonzaliseko Christine
- Date: 2010
- Subjects: Rhenium , Ligands
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10373 , http://hdl.handle.net/10948/1085 , Rhenium , Ligands
- Description: The reaction of the potentially tridentate Schiff-base chelate Hhaep [Haep = N’-(1- (2-hydroxyphenyl)ethylidene)benzohydrazide] with [ReCl3(benzil)(PPh3)] and trans-[ReOCl3(PPh3)2] produced the neutral oxorhenium(V) complexes cis- [ReOCl2(mep)] [Hmep = 2-(1-iminoethyl)phenol] and cis-[ReOCI2(meb)(PPh3)] [Hmeb = N’-(propan-2-ylidene)benzohydrazide] in ethanol and acetone respectively. In both reactions the Hhaep molecule cleaves to give different coordinated bidentate NO-donor chelates coordinated to the rhenium(V) centers. The X-ray studies reveal that mep is present as a bidentate, monoanionic Schiffbase coordinating through the neutral imino nitrogen and the deprotonated phenolate oxygen in cis-[ReOCl2(mep)]. The bond distances and angles in cis- [ReOCI2(meb)(PPh3)] confirm that meb coordinates to the metal in the enolate form. The distorted octahedral complex fac-[ReCl3(dpa)(PPh3)] was prepared by the reaction of trans-[ReCl3(MeCN)(PPh3)2] with a twofold molar excess of dpa in acetonitrile under a nitrogen atmosphere. The compound dpa.HCl.2H2O was obtained as a by-product in the reaction of dpa with trans-[ReCI3(MeCN)(PPh3)2] in acetonitrile. The reaction of trans-[ReCl3(MeCN)(PPh3)2] with a twofold molar excess of 6- amino-3-methyl-1-phenyl-4-azahept-2-ene-1-one (Hamp) in acetonitrile led to the isolation of cis-[ReCl2(bat)(PPh3)2]. On complexation to the metal center Hamp decomposed to give the coordinated benzoylacetone (bat). Bat is present as a monoanionic bidentate chelate. The complexes [ReVOCI(had)] and [ReIVCl(had)(PPh3)](ReO4) were prepared from the reaction of trans-[ReCl3(MeCN)(PPh3)2] with N,N-bis((2-hydroxybenzyl)-2- aminoethyl)dimethylamine (H2had) in ethanol under various reaction conditions. The treatment of [ReCl3(benzil)(PPh3)] with 2-[((2- pyridinylmethyl)amino)methyl]phenol (Hham) in a 2:1 molar ratio in acetonitrile led to the isolation of the hydrogen-bonded dimer [ReOCl2(ham)]2. The distorted octahedral complex [ReOCl(hap)] [H2hap = N,N-bis(2- hydroxybenzyl)aminomethylpyridine] was prepared from the reaction of trans- [ReCl3(MeCN)(PPh3)2] with a twofold molar excess of H2hap in acetonitrile. The X-ray crystal structure analysis shows that the chloride is coordinated trans to the tripodal tertiary amino nitrogen, with a phenolate oxygen trans to the oxo oxygen.
- Full Text:
- Date Issued: 2010
Sandy beach food webs and trophic linkages with estuaries: a stable light isotope approach
- Authors: Bezuidenhout, Karien
- Date: 2010
- Subjects: Stable isotopes in ecological research -- South Africa -- Eastern Cape , Food chains (Ecology) -- South Africa -- Eastern Cape , Estuaries -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10694 , http://hdl.handle.net/10948/1269 , Stable isotopes in ecological research -- South Africa -- Eastern Cape , Food chains (Ecology) -- South Africa -- Eastern Cape , Estuaries -- South Africa -- Eastern Cape
- Description: Two pocket sandy beaches, Eastern Cape, South Africa, were investigated, to determine whether these beaches were subsidised by an adjacent mangrove estuary, by using stable light isotope (δ 13C and δ 15N) analyses. The trophodynamics and macrofaunal food webs of these two beaches, situated between the Mgazi and Mgazana estuaries (in a warm-temperate/subtropical transition zone,), were described. Two to 2.5 trophic levels were identified for the macrobenthic community, with suspension feeders and omnivorous scavengers as the primary consumers, and carnivores as the secondary consumers. Mangrove material and terrigenous inputs were not driving the sandy beach food webs. Instead, marine allochthonous inputs (carrion, macroalgae), possibly phytoplankton, sediment organic matter, and resident macroinfauna were the dominant food sources. Cattle dung could have been the only important terrigenous food source utilised by the beach benthos. The macroinfauna displayed generalist/omnivorous feeding strategies, but within the limits of predominantly marine food sources. There was evidence that carnivores actively preyed on resident beach fauna. Omnivory and intraguild feeding might also be important biological processes in these communities. Seasonal and spatial variability in stable isotope composition of the fauna was observed, but few patterns were evident. There was a general trend of more enriched δ 15N and δ 13C composition of animal tissues in summer as opposed to winter. This was accompanied by a general decrease in C:N ratios in summer. It was hypothesised that these isotopic and biochemical changes were in response to increased food availability during summer. Although mangrove material appeared not to play an important role in the nutrition of these sandy beach communities, it was suggested that a high retention time of particles in the bay could enhance bacterial decay of particulate mangrove material, which could then act as fine, bacterial-enriched particulate food to the macrobenthos. This remains to be tested.
- Full Text:
- Date Issued: 2010
- Authors: Bezuidenhout, Karien
- Date: 2010
- Subjects: Stable isotopes in ecological research -- South Africa -- Eastern Cape , Food chains (Ecology) -- South Africa -- Eastern Cape , Estuaries -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10694 , http://hdl.handle.net/10948/1269 , Stable isotopes in ecological research -- South Africa -- Eastern Cape , Food chains (Ecology) -- South Africa -- Eastern Cape , Estuaries -- South Africa -- Eastern Cape
- Description: Two pocket sandy beaches, Eastern Cape, South Africa, were investigated, to determine whether these beaches were subsidised by an adjacent mangrove estuary, by using stable light isotope (δ 13C and δ 15N) analyses. The trophodynamics and macrofaunal food webs of these two beaches, situated between the Mgazi and Mgazana estuaries (in a warm-temperate/subtropical transition zone,), were described. Two to 2.5 trophic levels were identified for the macrobenthic community, with suspension feeders and omnivorous scavengers as the primary consumers, and carnivores as the secondary consumers. Mangrove material and terrigenous inputs were not driving the sandy beach food webs. Instead, marine allochthonous inputs (carrion, macroalgae), possibly phytoplankton, sediment organic matter, and resident macroinfauna were the dominant food sources. Cattle dung could have been the only important terrigenous food source utilised by the beach benthos. The macroinfauna displayed generalist/omnivorous feeding strategies, but within the limits of predominantly marine food sources. There was evidence that carnivores actively preyed on resident beach fauna. Omnivory and intraguild feeding might also be important biological processes in these communities. Seasonal and spatial variability in stable isotope composition of the fauna was observed, but few patterns were evident. There was a general trend of more enriched δ 15N and δ 13C composition of animal tissues in summer as opposed to winter. This was accompanied by a general decrease in C:N ratios in summer. It was hypothesised that these isotopic and biochemical changes were in response to increased food availability during summer. Although mangrove material appeared not to play an important role in the nutrition of these sandy beach communities, it was suggested that a high retention time of particles in the bay could enhance bacterial decay of particulate mangrove material, which could then act as fine, bacterial-enriched particulate food to the macrobenthos. This remains to be tested.
- Full Text:
- Date Issued: 2010
Spatial and temporal patterns in the hyperbenthic community structure in a permanently open Eastern Cape estuary, South Africa
- Authors: Heyns, Elodie R
- Date: 2010
- Subjects: Sundays Estuary -- South Africa -- Eastern Cape , Groundfishes -- South Africa -- Eatern Cape , Estuaries -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5768 , http://hdl.handle.net/10962/d1005456
- Description: The spatial and temporal (seasonal and diel) patterns in the hyperbenthic community structure (>500 μm) was investigated in the warm temperate, permanently open Kariega Estuary situated along the south-eastern coastline of South Africa. Spatial and seasonal patterns in the hyperbenthic community structure were assessed monthly at six stations along the length of the estuary over a period of twelve months. Data were collected using a modified hyperbenthic sledge, comprising two super-imposed nets. Physico-chemical data indicate the presence of a constant reverse salinity gradient, with highest salinities measured in the upper reaches and lowest at the mouth of the estuary. Strong seasonal patterns in temperature, dissolved oxygen and total chlorophyll-a (chl-a) concentrations were evident. Total average hyperbenthic densities ranged between 0.04 and 166 ind.m-3 in the lower net and between 0.12 and 225 ind.m-3 in the upper net. Hyperbenthic biomass values ranged between 0.02 and 11.9 mg.dry weight.m-3 in the lower net and between 0.02 and 17.4 mg.dry weight.m-3 in the upper net. A spatial and temporal pattern in total densities was detected with an increase in abundance over the period of September to October 2008 particularly in the middle reaches (Stations 3 and 4). Both the lower and upper nets were numerically dominated by decapods (mainly brachyuran crab zoeae) with the exception of June and July 2008 when mysids (mainly Mesopodopsis wooldridgei) dominated, making up 72.4 ± 58.14% of the total abundance in the lower net. A redundancy analysis (RDA) indicated that 99.2% of the variance in the hyperbenthic community structure could be explained by the first two canonical axes. Axis one, which accounted for 96.8% of the total variation detected in the ordination plot was highly correlated with sedimentary organic content and to a lesser extent the chl-a concentration within the Kariega Estuary. The correlations with the second canonical axis (2.4%) were less obvious, however, salinity and seston concentration were weakly correlated with this axis. Diel variability in the hyperbenthic community structure was assessed during March 2009. Samples were collected during the day and night (n = 6 for each period) using sampling gear described above. Total average hyperbenthic densities during the day (497.9 ± 254.1 ind.m-3) were significantly higher than night-time estimates (129.9 ± 38.5 ind.m-3; p<0.05). There were no significant differences in the average dayand night-time estimates of hyperbenthic biomass (p>0.05). A hierarchical cluster analysis identified two significantly distinct groupings, designated the day and night samples. Results from the SIMPER procedure indicated that the high densities of crab zoeae recorded during the day-time accounted for the majority of the dissimilarity between the day and night groupings (44.7%). In addition, it is apparent that several benthic species, especially from the cumacean and isopod orders, were absent from the hyperbenthos during the day-time and emerged into the water column at night.
- Full Text:
- Date Issued: 2010
- Authors: Heyns, Elodie R
- Date: 2010
- Subjects: Sundays Estuary -- South Africa -- Eastern Cape , Groundfishes -- South Africa -- Eatern Cape , Estuaries -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5768 , http://hdl.handle.net/10962/d1005456
- Description: The spatial and temporal (seasonal and diel) patterns in the hyperbenthic community structure (>500 μm) was investigated in the warm temperate, permanently open Kariega Estuary situated along the south-eastern coastline of South Africa. Spatial and seasonal patterns in the hyperbenthic community structure were assessed monthly at six stations along the length of the estuary over a period of twelve months. Data were collected using a modified hyperbenthic sledge, comprising two super-imposed nets. Physico-chemical data indicate the presence of a constant reverse salinity gradient, with highest salinities measured in the upper reaches and lowest at the mouth of the estuary. Strong seasonal patterns in temperature, dissolved oxygen and total chlorophyll-a (chl-a) concentrations were evident. Total average hyperbenthic densities ranged between 0.04 and 166 ind.m-3 in the lower net and between 0.12 and 225 ind.m-3 in the upper net. Hyperbenthic biomass values ranged between 0.02 and 11.9 mg.dry weight.m-3 in the lower net and between 0.02 and 17.4 mg.dry weight.m-3 in the upper net. A spatial and temporal pattern in total densities was detected with an increase in abundance over the period of September to October 2008 particularly in the middle reaches (Stations 3 and 4). Both the lower and upper nets were numerically dominated by decapods (mainly brachyuran crab zoeae) with the exception of June and July 2008 when mysids (mainly Mesopodopsis wooldridgei) dominated, making up 72.4 ± 58.14% of the total abundance in the lower net. A redundancy analysis (RDA) indicated that 99.2% of the variance in the hyperbenthic community structure could be explained by the first two canonical axes. Axis one, which accounted for 96.8% of the total variation detected in the ordination plot was highly correlated with sedimentary organic content and to a lesser extent the chl-a concentration within the Kariega Estuary. The correlations with the second canonical axis (2.4%) were less obvious, however, salinity and seston concentration were weakly correlated with this axis. Diel variability in the hyperbenthic community structure was assessed during March 2009. Samples were collected during the day and night (n = 6 for each period) using sampling gear described above. Total average hyperbenthic densities during the day (497.9 ± 254.1 ind.m-3) were significantly higher than night-time estimates (129.9 ± 38.5 ind.m-3; p<0.05). There were no significant differences in the average dayand night-time estimates of hyperbenthic biomass (p>0.05). A hierarchical cluster analysis identified two significantly distinct groupings, designated the day and night samples. Results from the SIMPER procedure indicated that the high densities of crab zoeae recorded during the day-time accounted for the majority of the dissimilarity between the day and night groupings (44.7%). In addition, it is apparent that several benthic species, especially from the cumacean and isopod orders, were absent from the hyperbenthos during the day-time and emerged into the water column at night.
- Full Text:
- Date Issued: 2010
Spatio-temporal dynamics of ichthyoplankton in the Kowie estuary, South Africa
- Authors: Kruger, Michelle
- Date: 2010
- Subjects: Fishes -- Larvae -- South Africa -- Port Alfred , Estuarine fishes -- South Africa -- Port Alfred
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5319 , http://hdl.handle.net/10962/d1005164 , Fishes -- Larvae -- South Africa -- Port Alfred , Estuarine fishes -- South Africa -- Port Alfred
- Description: Ichthyoplankton dynamics in the permanently open Kowie Estuary, in the warm temperate region of South Africa was investigated. The composition, abundance, distribution and seasonality of larval fishes were studied over a two year period between 2004 and 2006. Additionally, tidal exchange of ichthyoplankton and the use of frontal zones in the mouth region of the estuary were also explored between 2008 and 2009. Temporal and spatial trends in occurrence of larval fishes within the estuary and associated marina were obtained from data collected seasonally using boat-based plankton netting at 14 sampling stations along the length of the estuary. A total of 11 128 larval fishes were collected, representing 23 families and 38 taxa. Clupeidae and Gobiidae were the dominant fish families, contributing 47.0 % and 24.7 % respectively to the total catch. Estuarine resident species dominated the overall catch (91 %). A notable absence of older stage larvae and early juveniles characterised the artificial channels of the marina and estuary mouth region. This was attributed to the absence of a shallow, marginal water habitat typical of successful estuarine nursery areas. Tidal exchange of larval fishes was investigated in the Kowie Estuary using a new technique. A set of drifting light traps were set repetitively on the ebb and flood tide every second night for two consecutive 14 day periods during the peak estuarine recruitment period. A total of 553 larval fishes were caught during the study, representing nine families and 26 species. Blenniidae and Clupeidae dominated the catches. Family and species occurrence changed with tide state. Species richness (d) and diversity (H’) varied with tide and was highest on flood tides. Estuary-dependent species, such as Omobranchus woodii were more dominant on flood tides, whilst larvae of marine-spawned species, such as Sardinops sagax, were dominant on the ebb tide. Light trap catches yielded a different composition in terms of development stage / size and species, when compared to towed net studies. Towed plankton nets were again used to study the shear fronts that characterise the mouth region of the canalised Kowie Estuary. It was hypothesised that the convergence zone of the front provides feeding opportunities for ichthyoplankton. Ichthyoplankton, zooplankton and phytoplankton was collected from within and immediately outside of the convergence zone during frontal conditions. Species specific distribution trends emerged from this study. Postflexion larvae and early juvenile stages of the estuary dependent Mugilidae were only present in the foam line of the convergence zones. Some known predators of ichthyoplankton were also present in slightly higher numbers in the convergence zone (isopods) while other predators such as mysid shrimps, chaetognaths and cnidarians were more abundant out of the convergence zone. It appears that these zones may provide access to temporary food patches in the lower estuary but will be accompanied by a trade-off with increased isopod predators. The Kowie Estuary is a heavily impacted system and lack of adequate freshwater supply; artificial channelling and pollution ultimately have a negative impact of the success of this system as a nursery area. Rehabilitation of marginal areas in the lower estuary and marina is suggested as remedial action to re-establish the integrity of the nursery function this system could offer.
- Full Text:
- Date Issued: 2010
- Authors: Kruger, Michelle
- Date: 2010
- Subjects: Fishes -- Larvae -- South Africa -- Port Alfred , Estuarine fishes -- South Africa -- Port Alfred
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5319 , http://hdl.handle.net/10962/d1005164 , Fishes -- Larvae -- South Africa -- Port Alfred , Estuarine fishes -- South Africa -- Port Alfred
- Description: Ichthyoplankton dynamics in the permanently open Kowie Estuary, in the warm temperate region of South Africa was investigated. The composition, abundance, distribution and seasonality of larval fishes were studied over a two year period between 2004 and 2006. Additionally, tidal exchange of ichthyoplankton and the use of frontal zones in the mouth region of the estuary were also explored between 2008 and 2009. Temporal and spatial trends in occurrence of larval fishes within the estuary and associated marina were obtained from data collected seasonally using boat-based plankton netting at 14 sampling stations along the length of the estuary. A total of 11 128 larval fishes were collected, representing 23 families and 38 taxa. Clupeidae and Gobiidae were the dominant fish families, contributing 47.0 % and 24.7 % respectively to the total catch. Estuarine resident species dominated the overall catch (91 %). A notable absence of older stage larvae and early juveniles characterised the artificial channels of the marina and estuary mouth region. This was attributed to the absence of a shallow, marginal water habitat typical of successful estuarine nursery areas. Tidal exchange of larval fishes was investigated in the Kowie Estuary using a new technique. A set of drifting light traps were set repetitively on the ebb and flood tide every second night for two consecutive 14 day periods during the peak estuarine recruitment period. A total of 553 larval fishes were caught during the study, representing nine families and 26 species. Blenniidae and Clupeidae dominated the catches. Family and species occurrence changed with tide state. Species richness (d) and diversity (H’) varied with tide and was highest on flood tides. Estuary-dependent species, such as Omobranchus woodii were more dominant on flood tides, whilst larvae of marine-spawned species, such as Sardinops sagax, were dominant on the ebb tide. Light trap catches yielded a different composition in terms of development stage / size and species, when compared to towed net studies. Towed plankton nets were again used to study the shear fronts that characterise the mouth region of the canalised Kowie Estuary. It was hypothesised that the convergence zone of the front provides feeding opportunities for ichthyoplankton. Ichthyoplankton, zooplankton and phytoplankton was collected from within and immediately outside of the convergence zone during frontal conditions. Species specific distribution trends emerged from this study. Postflexion larvae and early juvenile stages of the estuary dependent Mugilidae were only present in the foam line of the convergence zones. Some known predators of ichthyoplankton were also present in slightly higher numbers in the convergence zone (isopods) while other predators such as mysid shrimps, chaetognaths and cnidarians were more abundant out of the convergence zone. It appears that these zones may provide access to temporary food patches in the lower estuary but will be accompanied by a trade-off with increased isopod predators. The Kowie Estuary is a heavily impacted system and lack of adequate freshwater supply; artificial channelling and pollution ultimately have a negative impact of the success of this system as a nursery area. Rehabilitation of marginal areas in the lower estuary and marina is suggested as remedial action to re-establish the integrity of the nursery function this system could offer.
- Full Text:
- Date Issued: 2010
Structure and functioning of fish assemblages in two South African estuaries, with emphasis on the presence and absence of aquatic macrophyte beds
- Authors: Sheppard, Jill Nicole
- Date: 2010
- Subjects: Estuaries -- South Africa -- Eastern Cape , Estuarine ecology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5321 , http://hdl.handle.net/10962/d1005166 , Estuaries -- South Africa -- Eastern Cape , Estuarine ecology -- South Africa -- Eastern Cape
- Description: Temporarily open/closed estuaries (TOCEs) are the dominant estuary type in South Africa. These systems are often characterized by extensive beds of submerged macrophytes, which form important foraging and shelter habitats for fishes, especially for estuary-dependent fish species such as the Cape stumpnose Rhabdosargus holubi and Cape moony Monodactylus falciformis that are commonly associated with them. A loss of submerged macrophytes from an estuary has been shown to affect the fish community as well as reducing overall system productivity. The TOC East Kleinemonde Estuary, situated in the warm-temperate biogeographic region of South Africa has been subject to an ongoing long-term fish monitoring project since 1995. During the period 1995 to 2002, this estuary contained large beds of the submerged macrophytes Ruppia cirrhosa and Potamogeton pectinatus. However, subsequent to a major flood event in 2003 these macrophytes have been largely absent from this system. The effect of the loss of submerged macrophytes on the East Kleinemonde fish assemblage was investigated through an analysis of seine and gill net catch data. Seine net catches for a 12 year period, encompassing six years of macrophyte presence and six years of macrophyte senescence, revealed changes in the relative abundance of certain fish species. Vegetation-associated species such as R. holubi and M. falciformis decreased in abundance whereas sediment-associated species, especially members of the family Mugilidae, increased in abundance following loss of the macrophytes in this estuary. The critically endangered pipefish Syngnathus watermeyeri was only recorded in catches during years in which macrophyte beds were present. In addition to the analysis of catch data, the importance of macrophytes as a primary energy source for selected estuarine fishes was explored through the analysis of carbon and nitrogen stable isotopes. Prior to conducting these analyses, common methodological practices to address the presence of carbonates and lipids within isotope samples were evaluated. A subset of samples were either acid washed to remove carbonates, or lipids were removed according to the method of Bligh and Dyer (1959) as both of these compounds have been shown to affect stable carbon and nitrogen isotope ratios. The suitability of the lipid normalization models of Fry (2002) and Post et al. (2007) for samples of estuarine fish muscle were also tested. Based on this evaluation both models are suitable for use with estuarine fish muscle tissue, however since neither carbonate nor lipid content of any of the samples used in this study was high all samples were left untreated in the following analysis. Carbon isotope ratios from a wide range of fish species collected from the East Kleinemonde Estuary during the macrophyte-senescent phase were compared with individuals of the same species from the neighbouring West Kleinemonde Estuary (where extensive beds of R. cirrhosa and P. pectinatus were present) and revealed the influence of submerged macrophyte material in the diet of fishes in the latter system. However, it was apparent that these plants are not directly consumed but rather contribute to the detrital pool that forms a food source for most invertebrate and some fish species. The most significant source of carbon for East Kleinemonde fishes during the macrophyte senescent phase appeared to have a more depleted origin; probably from benthic or pelagic microalgae. In conclusion, while the importance of macrophyte beds as shelter and foraging habitats for estuarine fishes are well documented, their role in terms of the structuring and functioning of fish assemblages in TOCEs remains somewhat uncertain. The findings of this study were possibly masked by the resilience of vegetation-associated species to the loss of this habitat, as well as by life history characteristics of species such as R. holubi that allow their numerical dominance despite habitat change. Nonetheless, macrophyte senescence in the East Kleinemonde Estuary resulted in the loss of at least one species and the reduced abundance of vegetation-associated species, probably reflective of reduced food resources and/or increased vulnerability to predation. As a result, beds of submerged macrophytes are an important habitat within TOCEs.
- Full Text:
- Date Issued: 2010
- Authors: Sheppard, Jill Nicole
- Date: 2010
- Subjects: Estuaries -- South Africa -- Eastern Cape , Estuarine ecology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5321 , http://hdl.handle.net/10962/d1005166 , Estuaries -- South Africa -- Eastern Cape , Estuarine ecology -- South Africa -- Eastern Cape
- Description: Temporarily open/closed estuaries (TOCEs) are the dominant estuary type in South Africa. These systems are often characterized by extensive beds of submerged macrophytes, which form important foraging and shelter habitats for fishes, especially for estuary-dependent fish species such as the Cape stumpnose Rhabdosargus holubi and Cape moony Monodactylus falciformis that are commonly associated with them. A loss of submerged macrophytes from an estuary has been shown to affect the fish community as well as reducing overall system productivity. The TOC East Kleinemonde Estuary, situated in the warm-temperate biogeographic region of South Africa has been subject to an ongoing long-term fish monitoring project since 1995. During the period 1995 to 2002, this estuary contained large beds of the submerged macrophytes Ruppia cirrhosa and Potamogeton pectinatus. However, subsequent to a major flood event in 2003 these macrophytes have been largely absent from this system. The effect of the loss of submerged macrophytes on the East Kleinemonde fish assemblage was investigated through an analysis of seine and gill net catch data. Seine net catches for a 12 year period, encompassing six years of macrophyte presence and six years of macrophyte senescence, revealed changes in the relative abundance of certain fish species. Vegetation-associated species such as R. holubi and M. falciformis decreased in abundance whereas sediment-associated species, especially members of the family Mugilidae, increased in abundance following loss of the macrophytes in this estuary. The critically endangered pipefish Syngnathus watermeyeri was only recorded in catches during years in which macrophyte beds were present. In addition to the analysis of catch data, the importance of macrophytes as a primary energy source for selected estuarine fishes was explored through the analysis of carbon and nitrogen stable isotopes. Prior to conducting these analyses, common methodological practices to address the presence of carbonates and lipids within isotope samples were evaluated. A subset of samples were either acid washed to remove carbonates, or lipids were removed according to the method of Bligh and Dyer (1959) as both of these compounds have been shown to affect stable carbon and nitrogen isotope ratios. The suitability of the lipid normalization models of Fry (2002) and Post et al. (2007) for samples of estuarine fish muscle were also tested. Based on this evaluation both models are suitable for use with estuarine fish muscle tissue, however since neither carbonate nor lipid content of any of the samples used in this study was high all samples were left untreated in the following analysis. Carbon isotope ratios from a wide range of fish species collected from the East Kleinemonde Estuary during the macrophyte-senescent phase were compared with individuals of the same species from the neighbouring West Kleinemonde Estuary (where extensive beds of R. cirrhosa and P. pectinatus were present) and revealed the influence of submerged macrophyte material in the diet of fishes in the latter system. However, it was apparent that these plants are not directly consumed but rather contribute to the detrital pool that forms a food source for most invertebrate and some fish species. The most significant source of carbon for East Kleinemonde fishes during the macrophyte senescent phase appeared to have a more depleted origin; probably from benthic or pelagic microalgae. In conclusion, while the importance of macrophyte beds as shelter and foraging habitats for estuarine fishes are well documented, their role in terms of the structuring and functioning of fish assemblages in TOCEs remains somewhat uncertain. The findings of this study were possibly masked by the resilience of vegetation-associated species to the loss of this habitat, as well as by life history characteristics of species such as R. holubi that allow their numerical dominance despite habitat change. Nonetheless, macrophyte senescence in the East Kleinemonde Estuary resulted in the loss of at least one species and the reduced abundance of vegetation-associated species, probably reflective of reduced food resources and/or increased vulnerability to predation. As a result, beds of submerged macrophytes are an important habitat within TOCEs.
- Full Text:
- Date Issued: 2010
Studies towards the development of novel HIV-1 integrase inhibitors
- Authors: Lee, Yi-Chen
- Date: 2010
- Subjects: HIV infections -- Treatment , HIV (Viruses) , AIDS (Disease) -- Treatment , Nuclear magnetic resonance , Heterocyclic compounds -- Derivatives , Enzyme inhibitors , Chemical inhibitors , Quinoline
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4357 , http://hdl.handle.net/10962/d1005022 , HIV infections -- Treatment , HIV (Viruses) , AIDS (Disease) -- Treatment , Nuclear magnetic resonance , Heterocyclic compounds -- Derivatives , Enzyme inhibitors , Chemical inhibitors , Quinoline
- Description: The project has focused on the preparation of several series of compounds designed as potential HIV-1 integrase inhibitors. Various 2-nitrobenzaldehydes have been reacted with two activated alkenes, methyl vinyl ketone (MVK) and methyl acrylate, under Baylis-Hillman conditions to afford α-methylene-β-hydroxylalkyl derivatives in moderate to excellent yields. The reactions were conducted using the tertiary amine catalysts, 1,4-diazabicyclo[2.2.2]octane(DABCO) or 3-hydroxyquinuclidine (3-HQ) with chloroform as solvent, and yields were optimised by varying the catalyst, reagent concentrations and the reaction time. Reductive cyclization of the Baylis-Hillman adducts via catalytic hydrogenation, using 10% palladiumon-carbon catalyst in ethanol, afforded quinoline and quinoline N-oxide derivatives. In some cases “acyclic” reduction products were also isolated. Reaction of the Baylis-Hillman MVK adducts with HCl, has resulted in effective nucleophilic (SN’) displacement of the hydroxyl group to afford allylic chloride derivatives. Direct substitution of these chloro derivatives by secondary or primary amines, followed by catalytic hydrogenation gave quinoline derivatives containing a 3-aminomethyl substituent. The Baylis-Hillman ester adducts obtained from reaction with methyl acrylate were treated directly with various amines to give diastereomeric conjugate addition products. Reactions with piperazine gave N,N’-disubstituted piperazine products. The piperidine derivatives have been dehydrated to give cinnamate esters in moderate yields. The products, which have all been satisfactorily characterised by elemental (HRMS) and spectroscopic (1- and 2-D NMR) analysis, constitute a “library” of compounds for in silico and in vitro studies as potential HIV integrase inhibitors.
- Full Text:
- Date Issued: 2010
- Authors: Lee, Yi-Chen
- Date: 2010
- Subjects: HIV infections -- Treatment , HIV (Viruses) , AIDS (Disease) -- Treatment , Nuclear magnetic resonance , Heterocyclic compounds -- Derivatives , Enzyme inhibitors , Chemical inhibitors , Quinoline
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4357 , http://hdl.handle.net/10962/d1005022 , HIV infections -- Treatment , HIV (Viruses) , AIDS (Disease) -- Treatment , Nuclear magnetic resonance , Heterocyclic compounds -- Derivatives , Enzyme inhibitors , Chemical inhibitors , Quinoline
- Description: The project has focused on the preparation of several series of compounds designed as potential HIV-1 integrase inhibitors. Various 2-nitrobenzaldehydes have been reacted with two activated alkenes, methyl vinyl ketone (MVK) and methyl acrylate, under Baylis-Hillman conditions to afford α-methylene-β-hydroxylalkyl derivatives in moderate to excellent yields. The reactions were conducted using the tertiary amine catalysts, 1,4-diazabicyclo[2.2.2]octane(DABCO) or 3-hydroxyquinuclidine (3-HQ) with chloroform as solvent, and yields were optimised by varying the catalyst, reagent concentrations and the reaction time. Reductive cyclization of the Baylis-Hillman adducts via catalytic hydrogenation, using 10% palladiumon-carbon catalyst in ethanol, afforded quinoline and quinoline N-oxide derivatives. In some cases “acyclic” reduction products were also isolated. Reaction of the Baylis-Hillman MVK adducts with HCl, has resulted in effective nucleophilic (SN’) displacement of the hydroxyl group to afford allylic chloride derivatives. Direct substitution of these chloro derivatives by secondary or primary amines, followed by catalytic hydrogenation gave quinoline derivatives containing a 3-aminomethyl substituent. The Baylis-Hillman ester adducts obtained from reaction with methyl acrylate were treated directly with various amines to give diastereomeric conjugate addition products. Reactions with piperazine gave N,N’-disubstituted piperazine products. The piperidine derivatives have been dehydrated to give cinnamate esters in moderate yields. The products, which have all been satisfactorily characterised by elemental (HRMS) and spectroscopic (1- and 2-D NMR) analysis, constitute a “library” of compounds for in silico and in vitro studies as potential HIV integrase inhibitors.
- Full Text:
- Date Issued: 2010
The anticancer activity of Cyathula prostrata on two malignant cell lines
- Authors: Schnablegger, Gerald
- Date: 2010
- Subjects: Medicinal plants
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10309 , http://hdl.handle.net/10948/1563 , Medicinal plants
- Description: Plants have always been a source of medicine and are still being used by traditional healers in the rural part of Africa, Asia and India to treat a range of illnesses including cancer. The in vitro anticancer activity of an 80 percent ethanol extract of Cyathula prostrata, an annual branching shrub used by traditional healers in Nigeria to treat cancer was investigated. No previous studies have outlined the possible pathways and mechanisms used by cancer cells when treated with C. prostrata. Dose response analysis was performed to determine the effective cytotoxic concentrations of C. prostrata on HeLa (cervical cancer cell line) and U937 (myelo-monocytic cell line). The IC50 values were 100.8 μg/ml and 64.4 μg/ml for HeLa and U937 cells, respectively. All further experiments were performed using 125 μg/ml C. prostrata extract and 50 μM cisplatin as positive control. With the use of the fluorescent DNA binding dye propidium iodide, the induction of tumour cell death by C. prostrata extract has been linked to cell cycle arrest in the G1 phase at 24 and 48 h. In both cell lines, more than 80 percent of the C. prostrata treated cells were found in the G1 phase after 48 hours of treatment. The annexin V-FITC/PI assay revealed an increase in the percentage apoptotic cells from 4.9 percent to 53.1 percent at 24 h and 8.3 percent to 50.3 percent at 48 h. Since apoptosis induction can occur via a number of different pathways, distinct features were used as markers to investigate the mode of action of this C. prostrata extract. Markers such as activated caspase-8, p21 and cyt-c, were investigated with the aid of fluorescently labelled (FITC) antibodies with analysis using flow cytometry. No change in p21 levels was observed in response to treatment with the extract for up to 48 h. Cell cycle arrest in G1 was therefore not induced by this cyclin-CDK inhibitor. Increase in caspase-8 activation was observed in response to treatment with the extract with no cyt-c release from the mitochondria. The lack of cyt-c release was due to no change in mitochondrial membrane potential, which was investigated with the aid of fluorescent mitochondrial dyes and flow cytometric techniques. Caspase-8 activation is unique to the extrinsic apoptotic pathway. The results from this study therefore show that C. prostrata extract induces apoptosis via the extrinsic pathway and that this activation in independent of the mitochondria. The levels of hTERT, the catalytic subunit of telomerase, were investigated as an additional molecular target for C. prostrata. This was also investigated using FITC labelled antibodies and flow cytometry. A decrease in hTERT levels was observed following C. prostrata treatment. The findings from this study suggest that the extract acts through multiple targets, by inducing: cell cycle arrest in the G1 phase through an unknown mechanism; apoptosis through an extrinsic death receptor pathway and replicative senescence through inhibition of telomerase.
- Full Text:
- Date Issued: 2010
- Authors: Schnablegger, Gerald
- Date: 2010
- Subjects: Medicinal plants
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10309 , http://hdl.handle.net/10948/1563 , Medicinal plants
- Description: Plants have always been a source of medicine and are still being used by traditional healers in the rural part of Africa, Asia and India to treat a range of illnesses including cancer. The in vitro anticancer activity of an 80 percent ethanol extract of Cyathula prostrata, an annual branching shrub used by traditional healers in Nigeria to treat cancer was investigated. No previous studies have outlined the possible pathways and mechanisms used by cancer cells when treated with C. prostrata. Dose response analysis was performed to determine the effective cytotoxic concentrations of C. prostrata on HeLa (cervical cancer cell line) and U937 (myelo-monocytic cell line). The IC50 values were 100.8 μg/ml and 64.4 μg/ml for HeLa and U937 cells, respectively. All further experiments were performed using 125 μg/ml C. prostrata extract and 50 μM cisplatin as positive control. With the use of the fluorescent DNA binding dye propidium iodide, the induction of tumour cell death by C. prostrata extract has been linked to cell cycle arrest in the G1 phase at 24 and 48 h. In both cell lines, more than 80 percent of the C. prostrata treated cells were found in the G1 phase after 48 hours of treatment. The annexin V-FITC/PI assay revealed an increase in the percentage apoptotic cells from 4.9 percent to 53.1 percent at 24 h and 8.3 percent to 50.3 percent at 48 h. Since apoptosis induction can occur via a number of different pathways, distinct features were used as markers to investigate the mode of action of this C. prostrata extract. Markers such as activated caspase-8, p21 and cyt-c, were investigated with the aid of fluorescently labelled (FITC) antibodies with analysis using flow cytometry. No change in p21 levels was observed in response to treatment with the extract for up to 48 h. Cell cycle arrest in G1 was therefore not induced by this cyclin-CDK inhibitor. Increase in caspase-8 activation was observed in response to treatment with the extract with no cyt-c release from the mitochondria. The lack of cyt-c release was due to no change in mitochondrial membrane potential, which was investigated with the aid of fluorescent mitochondrial dyes and flow cytometric techniques. Caspase-8 activation is unique to the extrinsic apoptotic pathway. The results from this study therefore show that C. prostrata extract induces apoptosis via the extrinsic pathway and that this activation in independent of the mitochondria. The levels of hTERT, the catalytic subunit of telomerase, were investigated as an additional molecular target for C. prostrata. This was also investigated using FITC labelled antibodies and flow cytometry. A decrease in hTERT levels was observed following C. prostrata treatment. The findings from this study suggest that the extract acts through multiple targets, by inducing: cell cycle arrest in the G1 phase through an unknown mechanism; apoptosis through an extrinsic death receptor pathway and replicative senescence through inhibition of telomerase.
- Full Text:
- Date Issued: 2010
The biology of austroglanis gilli and austroglanis barnardi (siluriformes : austroglanididae) in the Olifants River system, South Africa
- Authors: Mthombeni, Vusi Gedla
- Date: 2010
- Subjects: Catfishes -- South Africa -- Olifants River , Fishes -- South Africa -- Olifants River -- Growth , Freshwater fishes -- Effect of pesticides on -- South Africa -- Olifants River
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5370 , http://hdl.handle.net/10962/d1015222
- Description: Austroglanis gilli and A. barnardi are endemic to the Clanwilliam-Olifants System in the Western Cape, South Africa. The populations of each of these species are considered to be threatened by various anthropogenic activities, which include inappropriate agricultural practice and impacts of alien invasive fish species. The purpose of this thesis was to assess the life-history of these two endangered species in order to contribute to understanding their biology. Such information is vital for the development of strategies for their conservation. Marginal zone and marginal increment analyses from sectioned lapilliar otoliths of both A. gilli and A. barnardi showed a unimodal peak, suggesting a single annulus formation. The oldest specimens of A. gilli and A. barnardi were 12+ and 14+ years, respectively. The growth of A. gilli and A. barnardi was relatively slow and was best described by the von Bertalanffy growth curve as: L, = 131.56(1 - exp(- 0.27(t - 1.18 ))) for male and L, = 113.86(1 - exp(- 0.43(1- 0.74))) for female A. gilli from the Rondegat River. In the Noordhoeks River, growth was L, = 99.67(1 - exp(- 0.53(t - 0.35))) for male and L, = 96.60(1 - exp(- 0.64(t - 0.11 ))) for female A. gilli, and L, =71.02(1-exp(-0.26(1-3.07))) and L, = 69.50(1-exp(-0.36(1 - 1.88))) for male and female A. barnardi, respectively. The average natural mortality for the combined sexes was estimated using catch curve analysis at 0.37 ± 0.12 per year for A. gilli from the Rondegat River, and at 0.71 ± 0.05 and 0.39 ± 0.04 per year for A. gilli and A. barnardi from the Noordhoeks River, respectively. For A. gilli in the Rondegat River, the first maturity was estimated at 3.3 years (97.3 mm SL) for males and 3.1 years (94.3 mm SL) for females. In the Noordhoeks River, the first maturity for A. gilli was estimated at 2.0 years (71.9 mm SL) for males and 1.7 (66.4 mm SL) for females, and for A. barnardi at 2.0 (55.0 nun SL) for males and 2.9 years (58.9 mm SL) for females. Maturity corresponded closely to the asymptotic sizes from the von Bertalanffy curves, suggesting a shift in energy use from somatic growth to gonad development. Macroscopic assessment of the state of gonads, the Ganado-somatic index and histological examinations revealed that both A. gilli and A. barnardi have a single spawning season. The presence of oocytes in different stages of development in each of the ovaries of mature females collected between November and January suggested asynchronous, iteroperous serial spawning. The resorption of yolk was observed from ovaries collected between February and March and no vitellogenic oocyte was visible from ovaries collected between April and August. A similar trend was observed for males, with spermatozoa filling the lumen between November and January and some residual spermatozoa being present in the lumens soon after the breeding season. Spermatocytes and spermatids were dominant in the testes until October. An Index of Relative Importance revealed that A. gilli feeds predominantly on the benthic macroinvertebrate larvae of Ephemeroptera (particularly Baetidae), Diptera (particularly Chironomidae and Simuliidae) and Trichoptera. Austroglanis barnardi feeds predominantly on dipteran larvae (particularly Chironornidae and Simuliidae). Chi square contingency tables showed a significant difference in the dominant prey items of A. gilli and A. barnardi in the Noordhoeks River (X₂= 53.79, d.f. = 4, p > 0.001) and A. gilli between Rondegat and Noordhoeks rivers (x₂ = 34.74, d.f. = 4, p > 0.001). The Spearman's rank correlation test showed no shifts in the diet of A. barnardi from Noordhoeks River and A. gilli from Rondegat River with a change in size and season (p>O.OS). However, there was a shift in the diet of A. gilli from the Noordhoeks River which could suggest a distinct patchiness of benthic macroinvertebrates between the riffle feeding areas used by juveniles and other biotopes used by adults. The occurrence, in stomach contents, of other prey items from a wide variety of taxa and the presence of allochthonous material from the terrestrial environment could suggest an opportunistic feeding guild for both Austroglanis species. The life-history traits of A. gilli and A. barnardi, which are charaterized by slow growth, long life span and low relative fecundity, indicate that both species are relatively precocial and K-selected. The population of a precocial species is relatively stable and if population numbers were to be greatly reduced, they would require a long time to rebuild. An urgent conservation intervention is therefore recommended for Austroglanis spp. so as to maintain the diversity of populations within these species. The creation of protected river reserves and raising public conservation awareness may minimise activities that result in altered river hydrology and the destruction of complex benthic habitats.
- Full Text:
- Date Issued: 2010
- Authors: Mthombeni, Vusi Gedla
- Date: 2010
- Subjects: Catfishes -- South Africa -- Olifants River , Fishes -- South Africa -- Olifants River -- Growth , Freshwater fishes -- Effect of pesticides on -- South Africa -- Olifants River
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5370 , http://hdl.handle.net/10962/d1015222
- Description: Austroglanis gilli and A. barnardi are endemic to the Clanwilliam-Olifants System in the Western Cape, South Africa. The populations of each of these species are considered to be threatened by various anthropogenic activities, which include inappropriate agricultural practice and impacts of alien invasive fish species. The purpose of this thesis was to assess the life-history of these two endangered species in order to contribute to understanding their biology. Such information is vital for the development of strategies for their conservation. Marginal zone and marginal increment analyses from sectioned lapilliar otoliths of both A. gilli and A. barnardi showed a unimodal peak, suggesting a single annulus formation. The oldest specimens of A. gilli and A. barnardi were 12+ and 14+ years, respectively. The growth of A. gilli and A. barnardi was relatively slow and was best described by the von Bertalanffy growth curve as: L, = 131.56(1 - exp(- 0.27(t - 1.18 ))) for male and L, = 113.86(1 - exp(- 0.43(1- 0.74))) for female A. gilli from the Rondegat River. In the Noordhoeks River, growth was L, = 99.67(1 - exp(- 0.53(t - 0.35))) for male and L, = 96.60(1 - exp(- 0.64(t - 0.11 ))) for female A. gilli, and L, =71.02(1-exp(-0.26(1-3.07))) and L, = 69.50(1-exp(-0.36(1 - 1.88))) for male and female A. barnardi, respectively. The average natural mortality for the combined sexes was estimated using catch curve analysis at 0.37 ± 0.12 per year for A. gilli from the Rondegat River, and at 0.71 ± 0.05 and 0.39 ± 0.04 per year for A. gilli and A. barnardi from the Noordhoeks River, respectively. For A. gilli in the Rondegat River, the first maturity was estimated at 3.3 years (97.3 mm SL) for males and 3.1 years (94.3 mm SL) for females. In the Noordhoeks River, the first maturity for A. gilli was estimated at 2.0 years (71.9 mm SL) for males and 1.7 (66.4 mm SL) for females, and for A. barnardi at 2.0 (55.0 nun SL) for males and 2.9 years (58.9 mm SL) for females. Maturity corresponded closely to the asymptotic sizes from the von Bertalanffy curves, suggesting a shift in energy use from somatic growth to gonad development. Macroscopic assessment of the state of gonads, the Ganado-somatic index and histological examinations revealed that both A. gilli and A. barnardi have a single spawning season. The presence of oocytes in different stages of development in each of the ovaries of mature females collected between November and January suggested asynchronous, iteroperous serial spawning. The resorption of yolk was observed from ovaries collected between February and March and no vitellogenic oocyte was visible from ovaries collected between April and August. A similar trend was observed for males, with spermatozoa filling the lumen between November and January and some residual spermatozoa being present in the lumens soon after the breeding season. Spermatocytes and spermatids were dominant in the testes until October. An Index of Relative Importance revealed that A. gilli feeds predominantly on the benthic macroinvertebrate larvae of Ephemeroptera (particularly Baetidae), Diptera (particularly Chironomidae and Simuliidae) and Trichoptera. Austroglanis barnardi feeds predominantly on dipteran larvae (particularly Chironornidae and Simuliidae). Chi square contingency tables showed a significant difference in the dominant prey items of A. gilli and A. barnardi in the Noordhoeks River (X₂= 53.79, d.f. = 4, p > 0.001) and A. gilli between Rondegat and Noordhoeks rivers (x₂ = 34.74, d.f. = 4, p > 0.001). The Spearman's rank correlation test showed no shifts in the diet of A. barnardi from Noordhoeks River and A. gilli from Rondegat River with a change in size and season (p>O.OS). However, there was a shift in the diet of A. gilli from the Noordhoeks River which could suggest a distinct patchiness of benthic macroinvertebrates between the riffle feeding areas used by juveniles and other biotopes used by adults. The occurrence, in stomach contents, of other prey items from a wide variety of taxa and the presence of allochthonous material from the terrestrial environment could suggest an opportunistic feeding guild for both Austroglanis species. The life-history traits of A. gilli and A. barnardi, which are charaterized by slow growth, long life span and low relative fecundity, indicate that both species are relatively precocial and K-selected. The population of a precocial species is relatively stable and if population numbers were to be greatly reduced, they would require a long time to rebuild. An urgent conservation intervention is therefore recommended for Austroglanis spp. so as to maintain the diversity of populations within these species. The creation of protected river reserves and raising public conservation awareness may minimise activities that result in altered river hydrology and the destruction of complex benthic habitats.
- Full Text:
- Date Issued: 2010
The botanical importance and health of the Bushmans estuary, Eastern Cape, South Africa
- Authors: Jafta, Nolusindiso
- Date: 2010
- Subjects: Estuaries -- South Africa , Bushmans river estuary , Estuarine ecology -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10614 , http://hdl.handle.net/10948/1255 , Estuaries -- South Africa , Bushmans river estuary , Estuarine ecology -- South Africa
- Description: The Bushmans Estuary is one of the few permanently open estuaries in the Eastern Cape that is characterized by large intertidal salt marshes. Freshwater inflow to the estuary has decreased as a result of abstraction by more than 30 weirs and farm dams in the catchment. The mean annual run-off is naturally low (38 x 106 m3 y-1) and thus abstraction and reduction of freshwater inflow to the estuary is expected to cause a number of changes. The aims of this study were to determine the current health/status of the estuary based on the macrophytes and microalgae and identify monitoring indicators for the East London Department of Water Affairs, River Health Programme. Changes in the estuary over time were determined from available historical data which were compared with present data. This analysis showed that under normal average conditions freshwater inflow to the estuary is very low, less that 0.02 m3 s-1 most of the time. Under these conditions the estuary is in a homogenous marine state. Vertical and horizontal salinity gradients only form when high rainfall and run-off occurs (> 5 m3.s-1). Salinity gradients from 30.1 PSU at the mouth to 2.2 PSU in the upper reaches were measured in 2006 after a high flow event. However the estuary quickly reverted back to its homogenous condition within weeks after this flood. This study showed that freshwater inflow increased nutrient input to the estuary. Total oxidised nitrogen (TOxN) and soluble reactive phosphorus (SRP) concentrations were higher in August 2006, after the flood, than during the other low flow sampling sessions. TOxN decreased from a mean concentration of 21.6 μM in 2006 to 1.93 μM in February 2009. SRP decreased from 55.3 μM to 0.2 μM respectively. With the increased nutrient availability, the response in the estuary was an increase in phytoplankton biomass. After the 2006 floods the average water column chlorophyll-a was 9.0 μg l-1, while in the low freshwater inflow years it ranged from 2.1 to 4.8 μg l-1. The composition of the phytoplankton community was always dominated by flagellates and then diatoms, with higher cell numbers in the nutrient-enriched 2006 period. Although the water column nutrient data indicated that the estuary was oligotrophic, benthic microalgal biomass (11.9-16.1 μg.g-1) in the intertidal zone was comparable with nutrient rich estuaries. Benthic species indicative of polluted conditions were found (Nitzschia frustulum, Navicula gregaria, Navicula cryptotenelloides). These benthic species were found at the sites where wastewater / sewage seepage had occurred. Benthic diatom species also indicated freshwater inflow. During the high flow period in 2006 the dominant diatoms were fresh to brackish species that were strongly associated with the high concentrations of TOxN and SRP (Tryblionella constricta, Diploneis smithii, Hippodonta cf. gremainii, and Navicula species). During the freshwater limited period of 2008 and 2009 the benthic diatom species shifted to a group responding to the high salinity, ammonium and silicate concentrations. The species in this group were Nitzschia flexa, Navicula tenneloides, Diploneis elliptica, Amphora subacutiuscula and Nitzschia coarctata. Ordination results showed that the epiphytic diatom species responded to different environmental variables in the different years. Most of the species in 2008/2009 were associated with high salinity, temperature, dissolved oxygen, ammonium and silicate concentrations while the response was towards TOxN and SRP in 2006. The dominant species were Cocconeis placentula v euglyphyta in 2006; Nitzschia frustulum in 2008; and Synedra spp in 2009. The average biomass of the epiphytes was significantly lower in May 2008 than in both August 2006 and February 2009; 88.0 + 17.7 mg.m-2, 1.7 + 0.8 mg.m-2, and 61.8 + 14.4 mg.m-2 respectively. GIS mapping of past and present aerial photographs showed that submerged macrophyte (Zostera capensis) cover in 1966 and 1973 was less than that mapped for 2004. Salt marsh also increased its cover over time, from 86.9 ha in 1966 to 126 ha in 2004, colonizing what were bare sandy areas. Long-term monitoring of the health of the Bushmans Estuary should focus on salinity (as an indicator of inflow or deprivation of freshwater), benthic diatom identification and macrophyte distribution and composition (for the detection of pollution input), and bathymetric surveys (for shallowing of the estuary due to sedimentation).
- Full Text:
- Date Issued: 2010
- Authors: Jafta, Nolusindiso
- Date: 2010
- Subjects: Estuaries -- South Africa , Bushmans river estuary , Estuarine ecology -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10614 , http://hdl.handle.net/10948/1255 , Estuaries -- South Africa , Bushmans river estuary , Estuarine ecology -- South Africa
- Description: The Bushmans Estuary is one of the few permanently open estuaries in the Eastern Cape that is characterized by large intertidal salt marshes. Freshwater inflow to the estuary has decreased as a result of abstraction by more than 30 weirs and farm dams in the catchment. The mean annual run-off is naturally low (38 x 106 m3 y-1) and thus abstraction and reduction of freshwater inflow to the estuary is expected to cause a number of changes. The aims of this study were to determine the current health/status of the estuary based on the macrophytes and microalgae and identify monitoring indicators for the East London Department of Water Affairs, River Health Programme. Changes in the estuary over time were determined from available historical data which were compared with present data. This analysis showed that under normal average conditions freshwater inflow to the estuary is very low, less that 0.02 m3 s-1 most of the time. Under these conditions the estuary is in a homogenous marine state. Vertical and horizontal salinity gradients only form when high rainfall and run-off occurs (> 5 m3.s-1). Salinity gradients from 30.1 PSU at the mouth to 2.2 PSU in the upper reaches were measured in 2006 after a high flow event. However the estuary quickly reverted back to its homogenous condition within weeks after this flood. This study showed that freshwater inflow increased nutrient input to the estuary. Total oxidised nitrogen (TOxN) and soluble reactive phosphorus (SRP) concentrations were higher in August 2006, after the flood, than during the other low flow sampling sessions. TOxN decreased from a mean concentration of 21.6 μM in 2006 to 1.93 μM in February 2009. SRP decreased from 55.3 μM to 0.2 μM respectively. With the increased nutrient availability, the response in the estuary was an increase in phytoplankton biomass. After the 2006 floods the average water column chlorophyll-a was 9.0 μg l-1, while in the low freshwater inflow years it ranged from 2.1 to 4.8 μg l-1. The composition of the phytoplankton community was always dominated by flagellates and then diatoms, with higher cell numbers in the nutrient-enriched 2006 period. Although the water column nutrient data indicated that the estuary was oligotrophic, benthic microalgal biomass (11.9-16.1 μg.g-1) in the intertidal zone was comparable with nutrient rich estuaries. Benthic species indicative of polluted conditions were found (Nitzschia frustulum, Navicula gregaria, Navicula cryptotenelloides). These benthic species were found at the sites where wastewater / sewage seepage had occurred. Benthic diatom species also indicated freshwater inflow. During the high flow period in 2006 the dominant diatoms were fresh to brackish species that were strongly associated with the high concentrations of TOxN and SRP (Tryblionella constricta, Diploneis smithii, Hippodonta cf. gremainii, and Navicula species). During the freshwater limited period of 2008 and 2009 the benthic diatom species shifted to a group responding to the high salinity, ammonium and silicate concentrations. The species in this group were Nitzschia flexa, Navicula tenneloides, Diploneis elliptica, Amphora subacutiuscula and Nitzschia coarctata. Ordination results showed that the epiphytic diatom species responded to different environmental variables in the different years. Most of the species in 2008/2009 were associated with high salinity, temperature, dissolved oxygen, ammonium and silicate concentrations while the response was towards TOxN and SRP in 2006. The dominant species were Cocconeis placentula v euglyphyta in 2006; Nitzschia frustulum in 2008; and Synedra spp in 2009. The average biomass of the epiphytes was significantly lower in May 2008 than in both August 2006 and February 2009; 88.0 + 17.7 mg.m-2, 1.7 + 0.8 mg.m-2, and 61.8 + 14.4 mg.m-2 respectively. GIS mapping of past and present aerial photographs showed that submerged macrophyte (Zostera capensis) cover in 1966 and 1973 was less than that mapped for 2004. Salt marsh also increased its cover over time, from 86.9 ha in 1966 to 126 ha in 2004, colonizing what were bare sandy areas. Long-term monitoring of the health of the Bushmans Estuary should focus on salinity (as an indicator of inflow or deprivation of freshwater), benthic diatom identification and macrophyte distribution and composition (for the detection of pollution input), and bathymetric surveys (for shallowing of the estuary due to sedimentation).
- Full Text:
- Date Issued: 2010
The dynamics of Larval fish and Zooplankton assemblages in the Sundays Estuary, South Africa
- Authors: Sutherland, Kate
- Date: 2010
- Subjects: Marine zooplankton -- South Africa , Zooplankton -- South Africa , Fishes -- Larvae -- South Africa , Estuarine ecology -- South Africa , Estuaries -- South Africa -- Management , Environmental monitoring -- South Africa , Coastal zone management -- South Africa , Estuarine area conservation -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10697 , http://hdl.handle.net/10948/1119 , Marine zooplankton -- South Africa , Zooplankton -- South Africa , Fishes -- Larvae -- South Africa , Estuarine ecology -- South Africa , Estuaries -- South Africa -- Management , Environmental monitoring -- South Africa , Coastal zone management -- South Africa , Estuarine area conservation -- South Africa
- Description: The larval fish and zooplankton assemblages were studied in the permanently open Sundays Estuary on the south-east coast of South Africa, using standard boat-based plankton towing methods. A total of 8174 larval and early juvenile fishes were caught, representing 12 families and 23 taxa. The Clupeidae, Gobiidae and Blenniidae were the dominant fish families. Common species included Gilchristella aestuaria, Caffrogobius gilchristi, Omobranchus woodi, Liza dumerilii, Glossogobius callidus and Myxus capensis. Estuarine resident species (Category I) predominantly in the preflexion developmental stage, dominated the system. A total of 19 zooplankton taxa were recorded. Copepoda dominated the zooplankton community. Dominant species included Pseudodiaptomus hessei, Acartia longipatella, Halicyclops sp., Mesopodopsis wooldridgei, and the larvae of Paratylodiplax edwardsii and Hymenosoma orbiculare. Mean larval fish density showed similar trends seasonally, spatially and across salinity zones, with mean zooplankton density in the Sundays Estuary. Gut content analysis of five larval fish species: Gilchristella aestuaria, Pomadasys commersonnii, Monodactylus falciformis, Myxus capensis and Rhabdosargus holubi, revealed species specific diet and prey selection. Although larval fish diet contained a variety of prey items, guts were dominated by P. hessei, chironomid larvae, Corophium triaenonyx, copepod eggs and insect larvae. Physico-chemical drivers and the interactions between these two plankton communities provide information that enables a more holistic view of the dynamics occurring in the Sundays Estuary planktonic ecosystem.
- Full Text:
- Date Issued: 2010
- Authors: Sutherland, Kate
- Date: 2010
- Subjects: Marine zooplankton -- South Africa , Zooplankton -- South Africa , Fishes -- Larvae -- South Africa , Estuarine ecology -- South Africa , Estuaries -- South Africa -- Management , Environmental monitoring -- South Africa , Coastal zone management -- South Africa , Estuarine area conservation -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10697 , http://hdl.handle.net/10948/1119 , Marine zooplankton -- South Africa , Zooplankton -- South Africa , Fishes -- Larvae -- South Africa , Estuarine ecology -- South Africa , Estuaries -- South Africa -- Management , Environmental monitoring -- South Africa , Coastal zone management -- South Africa , Estuarine area conservation -- South Africa
- Description: The larval fish and zooplankton assemblages were studied in the permanently open Sundays Estuary on the south-east coast of South Africa, using standard boat-based plankton towing methods. A total of 8174 larval and early juvenile fishes were caught, representing 12 families and 23 taxa. The Clupeidae, Gobiidae and Blenniidae were the dominant fish families. Common species included Gilchristella aestuaria, Caffrogobius gilchristi, Omobranchus woodi, Liza dumerilii, Glossogobius callidus and Myxus capensis. Estuarine resident species (Category I) predominantly in the preflexion developmental stage, dominated the system. A total of 19 zooplankton taxa were recorded. Copepoda dominated the zooplankton community. Dominant species included Pseudodiaptomus hessei, Acartia longipatella, Halicyclops sp., Mesopodopsis wooldridgei, and the larvae of Paratylodiplax edwardsii and Hymenosoma orbiculare. Mean larval fish density showed similar trends seasonally, spatially and across salinity zones, with mean zooplankton density in the Sundays Estuary. Gut content analysis of five larval fish species: Gilchristella aestuaria, Pomadasys commersonnii, Monodactylus falciformis, Myxus capensis and Rhabdosargus holubi, revealed species specific diet and prey selection. Although larval fish diet contained a variety of prey items, guts were dominated by P. hessei, chironomid larvae, Corophium triaenonyx, copepod eggs and insect larvae. Physico-chemical drivers and the interactions between these two plankton communities provide information that enables a more holistic view of the dynamics occurring in the Sundays Estuary planktonic ecosystem.
- Full Text:
- Date Issued: 2010
The E.coli RNA degradosome analysis of molecular chaperones and enolase
- Authors: Burger, Adélle
- Date: 2010
- Subjects: Molecular chaperones , Escherichia coli -- Biotechnology , Polyphosphates , Polyphosphates -- Biotechnology , RNA-protein interactions
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3950 , http://hdl.handle.net/10962/d1004009 , Molecular chaperones , Escherichia coli -- Biotechnology , Polyphosphates , Polyphosphates -- Biotechnology , RNA-protein interactions
- Description: Normal mRNA turnover is essential for genetic regulation within cells. The E. coli RNA degradosome, a large multi-component protein complex which originates through specific protein interactions, has been referred to as the “RNA decay machine” and is responsible for mRNA turnover. The degradosome functions to process RNA and its key components have been identified. The scaffold protein is RNase E and it tethers the degradosome to the cytoplasmic membrane. Polynucleotide phosphorylase (PNPase), ATP-dependent RNA helicase (RhlB helicase) and the glycolytic enzyme enolase associate with RNase E to form the degradosome. Polyphosphate kinase associates with the degradosome in substoichiometric amounts, as do the molecular chaperones DnaK and GroEL. The role of DnaK as well as that of enolase in the RNA degradosome is unknown. Very limited research has been conducted on the components of the RNA degradosome under conditions of stress. The aim of this study was to understand the role played by enolase in the assembly of the degradosome under conditions of stress, as well as investigating the protein levels of molecular chaperones under these conditions. The RNA degradosome was successfully purified through its scaffold protein using nickel-affinity chromatography. In vivo studies were performed to investigate the protein levels of DnaK and GroEL present in the degradosome under conditions of heat stress, and whether GroEL could functionally replace DnaK in the degradosome. To investigate the recruitment of enolase to the degradosome under heat stress, a subcellular fractionation was performed to determine the localization of enolase upon heat shock in vivo. The elevated temperature resulted in an increased concentration of enolase in the membrane fraction. To determine whether there is an interaction between enolase and DnaK, enolase activity assays were conducted in vitro. The effect of DnaK on enolase activity was measured upon quantifying DnaK and adding it to the enolase assays. For the first time it was observed that the activity of enolase increased with the addition of substoichiometric amounts of DnaK. This indicates that DnaK may be interacting with the RNA degradosome via enolase.
- Full Text:
- Date Issued: 2010
- Authors: Burger, Adélle
- Date: 2010
- Subjects: Molecular chaperones , Escherichia coli -- Biotechnology , Polyphosphates , Polyphosphates -- Biotechnology , RNA-protein interactions
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3950 , http://hdl.handle.net/10962/d1004009 , Molecular chaperones , Escherichia coli -- Biotechnology , Polyphosphates , Polyphosphates -- Biotechnology , RNA-protein interactions
- Description: Normal mRNA turnover is essential for genetic regulation within cells. The E. coli RNA degradosome, a large multi-component protein complex which originates through specific protein interactions, has been referred to as the “RNA decay machine” and is responsible for mRNA turnover. The degradosome functions to process RNA and its key components have been identified. The scaffold protein is RNase E and it tethers the degradosome to the cytoplasmic membrane. Polynucleotide phosphorylase (PNPase), ATP-dependent RNA helicase (RhlB helicase) and the glycolytic enzyme enolase associate with RNase E to form the degradosome. Polyphosphate kinase associates with the degradosome in substoichiometric amounts, as do the molecular chaperones DnaK and GroEL. The role of DnaK as well as that of enolase in the RNA degradosome is unknown. Very limited research has been conducted on the components of the RNA degradosome under conditions of stress. The aim of this study was to understand the role played by enolase in the assembly of the degradosome under conditions of stress, as well as investigating the protein levels of molecular chaperones under these conditions. The RNA degradosome was successfully purified through its scaffold protein using nickel-affinity chromatography. In vivo studies were performed to investigate the protein levels of DnaK and GroEL present in the degradosome under conditions of heat stress, and whether GroEL could functionally replace DnaK in the degradosome. To investigate the recruitment of enolase to the degradosome under heat stress, a subcellular fractionation was performed to determine the localization of enolase upon heat shock in vivo. The elevated temperature resulted in an increased concentration of enolase in the membrane fraction. To determine whether there is an interaction between enolase and DnaK, enolase activity assays were conducted in vitro. The effect of DnaK on enolase activity was measured upon quantifying DnaK and adding it to the enolase assays. For the first time it was observed that the activity of enolase increased with the addition of substoichiometric amounts of DnaK. This indicates that DnaK may be interacting with the RNA degradosome via enolase.
- Full Text:
- Date Issued: 2010
The effects of booster breaks during a sedentary night shift on physiological, psychomotor, psycho-physiological, and cognitive performance over a 3 night shift habituation phase
- Authors: Lombard, Wesley Ross
- Date: 2010
- Subjects: Night work , Shift systems , Performance , Exercise , Exercise -- Physiological aspects , Exercise -- Psychological aspects , Cognition -- Effect of exercise on , Motor ability
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5116 , http://hdl.handle.net/10962/d1005194 , Night work , Shift systems , Performance , Exercise , Exercise -- Physiological aspects , Exercise -- Psychological aspects , Cognition -- Effect of exercise on , Motor ability
- Description: Despite extensive research into shift work, workers working under rotating shift conditions are still plagued by the effects of the desynchronisation resulting from working against their natural circadian rhythms. Additionally, modern industries are shifting towards tasks requiring greater cognitive demand with less manual labour incorporated into the tasks. Research into operator based tasks, and hence those of a sedentary cognitive base both during day and night shifts, has been focusing on the effectiveness of the standard rest/break schedule. Research indicating that the standard rest break schedule is often ineffective in eliminating operator discomfort and performance deterioration, with these affects argued to be more pronounced during a night shift schedule. Therefore current research set out to investigate alternative rest break schedules, incorporating a short bout of physical activity and stretching exercises which are proposed to enhance performance and subjective mood, while eliminating operator discomfort for sedentary based cognitive tasks. Three conditions were tested during a three day habituation shift cycle within a laboratory, incorporating two night shift groups (control and experimental) and a control day shift group. Twelve subjects made up each group, with the two night shift groups completing the shift schedule together. The control groups followed a typical 8 hour shift schedule while the experimental group performed a booster break (exercise and stretches) activity for 7.5 minutes every hour during the night shift schedule. Over the course of the shift, subjects completed a battery of six tests providing data on physiological measurements (heart rate and temperature), performance criteria (reaction time responses, memory and neurobiological) and subjective measures. Responses obtained for all the different parameters measured indicated a strong circadian influence for the majority of the variables, indicating the course of natural down regulation within physiological and performance criteria over the night shift. The booster break significantly improved reaction time performance, subjective ratings and resulted in a high sustainable activity level. Day shift comparisons indicating that within subjective measures and reaction time performance, the booster break resulted in similar responses to those of the day shift workers, while the control night shift groups reported significantly lowers results. Additionally, the booster break had positive influences during the circadian nadir, significantly improving parameters of performance and subjective ratings of sleepiness. The results of this study indicating which variables are strong predictors and indicators of the oscillations in performance and subjective ratings due to the circadian changes. The booster break interventions had positive effects on subjective ratings and reaction time performance, while also being argued to decrease the burden placed on the cardiac system as a result of increased sympathetic tone during the night shift, while additionally resulting in similar responses to those of day shift workers. Further studies are required, however, to provide conclusive evidence particularly within a working situation over a longer shift schedule.
- Full Text:
- Date Issued: 2010
- Authors: Lombard, Wesley Ross
- Date: 2010
- Subjects: Night work , Shift systems , Performance , Exercise , Exercise -- Physiological aspects , Exercise -- Psychological aspects , Cognition -- Effect of exercise on , Motor ability
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5116 , http://hdl.handle.net/10962/d1005194 , Night work , Shift systems , Performance , Exercise , Exercise -- Physiological aspects , Exercise -- Psychological aspects , Cognition -- Effect of exercise on , Motor ability
- Description: Despite extensive research into shift work, workers working under rotating shift conditions are still plagued by the effects of the desynchronisation resulting from working against their natural circadian rhythms. Additionally, modern industries are shifting towards tasks requiring greater cognitive demand with less manual labour incorporated into the tasks. Research into operator based tasks, and hence those of a sedentary cognitive base both during day and night shifts, has been focusing on the effectiveness of the standard rest/break schedule. Research indicating that the standard rest break schedule is often ineffective in eliminating operator discomfort and performance deterioration, with these affects argued to be more pronounced during a night shift schedule. Therefore current research set out to investigate alternative rest break schedules, incorporating a short bout of physical activity and stretching exercises which are proposed to enhance performance and subjective mood, while eliminating operator discomfort for sedentary based cognitive tasks. Three conditions were tested during a three day habituation shift cycle within a laboratory, incorporating two night shift groups (control and experimental) and a control day shift group. Twelve subjects made up each group, with the two night shift groups completing the shift schedule together. The control groups followed a typical 8 hour shift schedule while the experimental group performed a booster break (exercise and stretches) activity for 7.5 minutes every hour during the night shift schedule. Over the course of the shift, subjects completed a battery of six tests providing data on physiological measurements (heart rate and temperature), performance criteria (reaction time responses, memory and neurobiological) and subjective measures. Responses obtained for all the different parameters measured indicated a strong circadian influence for the majority of the variables, indicating the course of natural down regulation within physiological and performance criteria over the night shift. The booster break significantly improved reaction time performance, subjective ratings and resulted in a high sustainable activity level. Day shift comparisons indicating that within subjective measures and reaction time performance, the booster break resulted in similar responses to those of the day shift workers, while the control night shift groups reported significantly lowers results. Additionally, the booster break had positive influences during the circadian nadir, significantly improving parameters of performance and subjective ratings of sleepiness. The results of this study indicating which variables are strong predictors and indicators of the oscillations in performance and subjective ratings due to the circadian changes. The booster break interventions had positive effects on subjective ratings and reaction time performance, while also being argued to decrease the burden placed on the cardiac system as a result of increased sympathetic tone during the night shift, while additionally resulting in similar responses to those of day shift workers. Further studies are required, however, to provide conclusive evidence particularly within a working situation over a longer shift schedule.
- Full Text:
- Date Issued: 2010
The effects of glove fit on task performance and on the human operator
- Authors: Stack, Jessica Danielle
- Date: 2010
- Subjects: Hand -- Anatomy , Hand -- Wounds and injuries , Hand -- Care and hygiene , Gloves , Safety education, Industrial , Human-machine systems , Industrial safety , Industrial accidents
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5119 , http://hdl.handle.net/10962/d1005197 , Hand -- Anatomy , Hand -- Wounds and injuries , Hand -- Care and hygiene , Gloves , Safety education, Industrial , Human-machine systems , Industrial safety , Industrial accidents
- Description: The hand is one of the most complex of all of the anatomical structures in the human body. It has been found that hand injuries are among the most frequent injuries that occur to the body, predominantly during industrial activities. It has therefore been concluded that more research is needed into protective factors, such as glove use. The design features of a glove emphasise either protection or performance. There is often a trade-off between increased safety and performance capability when donning gloves. It has been determined that gloves which are fitted and comfortable for the worker may provide the best compromise between protective functions and decreased performance. This investigation aimed to assess the influence of glove fit on the performance attributes of industrial tasks, as well as on the responses of the human operator. Glove fit was analysed as 35 male participants donned three different glove sizes during each test, including a best-fitting glove, a glove one size smaller than best-fitting, and a glove one size larger than best-fitting. For each glove size, gloves of two differing materials were tested, namely nitrile and neoprene. A barehanded condition was also tested, totalling seven gloved/barehanded conditions for each test. The seven conditions were assessed in a laboratory setting in a battery of tests. This consisted of components of task performance, including maximum pulling and pushing force, maximum torque, precision of force, tactility, speed and accuracy and dexterity. The performance responses were recorded, as well as participants’ perceptual responses using the Rating of Perceived Exertion scale, and muscle activity. Six muscles were selected: Flexor Digitorum Superficialis, Flexor Pollicus Longus, Extensor Carpi Ulnaris, Extensor Carpi Radialis, Flexor Carpi Ulnaris and Flexor Carpi Radialis. The results revealed that glove fit does affect certain spects of performance, and influences human operator responses for selected task components. Furthermore, discrepancies were distinguished between orking barehanded and working with an optimally fitted glove. There was also a glove material effect established. Overall, it was found that muscle activity when exerting maximum force in a pushing and pulling direction was optimal with the nitrile glove material. Maximum torque performance was enhanced with the use of a best-fitting glove, as compared with an ill-fitting glove or barehanded work. Force precision was preferable when barehanded, as opposed to the tactility task which rendered optimal results with a best-fitting glove. The same was found for speed and accuracy results, as glove fit appeared to have no effect on performance, but performance was improved when participants were barehanded. Dexterity performance was the most conclusively influenced by the conditions, resulting in barehanded performance being optimal. However, should a glove be necessary for a given task, an optimally-fitted glove which is of a thinner material would be recommended. It is necessary to distinguish the performance components of a task within industry and select the most appropriate glove for optimal performance and the least risk of overexertion.
- Full Text:
- Date Issued: 2010
- Authors: Stack, Jessica Danielle
- Date: 2010
- Subjects: Hand -- Anatomy , Hand -- Wounds and injuries , Hand -- Care and hygiene , Gloves , Safety education, Industrial , Human-machine systems , Industrial safety , Industrial accidents
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5119 , http://hdl.handle.net/10962/d1005197 , Hand -- Anatomy , Hand -- Wounds and injuries , Hand -- Care and hygiene , Gloves , Safety education, Industrial , Human-machine systems , Industrial safety , Industrial accidents
- Description: The hand is one of the most complex of all of the anatomical structures in the human body. It has been found that hand injuries are among the most frequent injuries that occur to the body, predominantly during industrial activities. It has therefore been concluded that more research is needed into protective factors, such as glove use. The design features of a glove emphasise either protection or performance. There is often a trade-off between increased safety and performance capability when donning gloves. It has been determined that gloves which are fitted and comfortable for the worker may provide the best compromise between protective functions and decreased performance. This investigation aimed to assess the influence of glove fit on the performance attributes of industrial tasks, as well as on the responses of the human operator. Glove fit was analysed as 35 male participants donned three different glove sizes during each test, including a best-fitting glove, a glove one size smaller than best-fitting, and a glove one size larger than best-fitting. For each glove size, gloves of two differing materials were tested, namely nitrile and neoprene. A barehanded condition was also tested, totalling seven gloved/barehanded conditions for each test. The seven conditions were assessed in a laboratory setting in a battery of tests. This consisted of components of task performance, including maximum pulling and pushing force, maximum torque, precision of force, tactility, speed and accuracy and dexterity. The performance responses were recorded, as well as participants’ perceptual responses using the Rating of Perceived Exertion scale, and muscle activity. Six muscles were selected: Flexor Digitorum Superficialis, Flexor Pollicus Longus, Extensor Carpi Ulnaris, Extensor Carpi Radialis, Flexor Carpi Ulnaris and Flexor Carpi Radialis. The results revealed that glove fit does affect certain spects of performance, and influences human operator responses for selected task components. Furthermore, discrepancies were distinguished between orking barehanded and working with an optimally fitted glove. There was also a glove material effect established. Overall, it was found that muscle activity when exerting maximum force in a pushing and pulling direction was optimal with the nitrile glove material. Maximum torque performance was enhanced with the use of a best-fitting glove, as compared with an ill-fitting glove or barehanded work. Force precision was preferable when barehanded, as opposed to the tactility task which rendered optimal results with a best-fitting glove. The same was found for speed and accuracy results, as glove fit appeared to have no effect on performance, but performance was improved when participants were barehanded. Dexterity performance was the most conclusively influenced by the conditions, resulting in barehanded performance being optimal. However, should a glove be necessary for a given task, an optimally-fitted glove which is of a thinner material would be recommended. It is necessary to distinguish the performance components of a task within industry and select the most appropriate glove for optimal performance and the least risk of overexertion.
- Full Text:
- Date Issued: 2010
The geology of the Witteberg group, Cape supergroup, with specific focus on the Perdepoort member as a potential silica source
- Authors: Olivier, Wernich Corné
- Date: 2010
- Subjects: Geology -- South Africa -- Witteberg Group , Stratiography -- South Africa -- Witteberg Group , Silicate minerals -- South Africa -- Witteberg Group
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10660 , http://hdl.handle.net/10948/1386 , http://hdl.handle.net/10948/d1012889 , Geology -- South Africa -- Witteberg Group , Stratiography -- South Africa -- Witteberg Group , Silicate minerals -- South Africa -- Witteberg Group
- Description: Selected outcrops of the Upper Devonian to Lower Carboniferous, Witteberg Group, Cape Supergroup were mineralogically and structurally analyzed. The study area is located approximately 30km northwest of Kirkwood and 10km south of Darlington Dam, Eastern Cape, South Africa. Strata predominantly consist of arenaceous Witpoort Formation, which includes the Perdepoort, and Rooirand Members. The Perdepoort Member is a thinly bedded quartzite also known as the "white streak". The Rooirand Member quartzite is a highly iron stained red-brown quartzite. The dark-grey, pyritic rich shales of the Kweekvlei Formation overlie the Witpoort Formation in the southern half of the study site. These shales are highly deformed and display closely spaced thrust faults and close folds. The study area encapsulates a range of folding from tight to open folds. Faulting consists of low angle north verging thrust fault, south verging back thrusts, south and north dipping normal faults, and strike-slip faults. Closely spaced, fore-land verging thrusts faults predominate over hinterland verging back thrusts. Normal faulting post-dates thrust faulting and utilized weaknesses in axial planar cleavage and in certain instances existing thrust fault planes. Strike-slip faulting post-dates thrusting and has in places reactivated pre-existing thrust fault planes. Macro scale folding includes overturned synclines and large anticlines which have been eroded, exposing older strata. Fold axes plunge at low to moderate angles west-southwest. This correlates with tension gashes which indicate north westward directed forces. Eastward directed forces are confirmed by the presence of tension gashes and strike-slip movement. The local geology displays north westward directed compression followed by strike-slip movement. Normal faulting post-dates all other structures and is associated with the Mesozoic break-up of Gondwana. The Perdepoort Member was sampled along strike, at different outcrop latitudes. Seven samples were selected for scanning electron microscope analysis. Samples are composed almost entirely of quartz; accessories include, biotite, muscovite, sericite, baryte, and apatite. Epigenetic hematite is present along cracks within certain samples Epigenetic hematite occur along cracks with oxides and phosphates in the form of rutile, apatite and monazite present in a number of samples. When compared to other silica extraction operations the Perdepoort Member appears viable for explotation. However, for the solar cell industry the purity of this horizon is clearly far below that required for industy.
- Full Text:
- Date Issued: 2010
- Authors: Olivier, Wernich Corné
- Date: 2010
- Subjects: Geology -- South Africa -- Witteberg Group , Stratiography -- South Africa -- Witteberg Group , Silicate minerals -- South Africa -- Witteberg Group
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10660 , http://hdl.handle.net/10948/1386 , http://hdl.handle.net/10948/d1012889 , Geology -- South Africa -- Witteberg Group , Stratiography -- South Africa -- Witteberg Group , Silicate minerals -- South Africa -- Witteberg Group
- Description: Selected outcrops of the Upper Devonian to Lower Carboniferous, Witteberg Group, Cape Supergroup were mineralogically and structurally analyzed. The study area is located approximately 30km northwest of Kirkwood and 10km south of Darlington Dam, Eastern Cape, South Africa. Strata predominantly consist of arenaceous Witpoort Formation, which includes the Perdepoort, and Rooirand Members. The Perdepoort Member is a thinly bedded quartzite also known as the "white streak". The Rooirand Member quartzite is a highly iron stained red-brown quartzite. The dark-grey, pyritic rich shales of the Kweekvlei Formation overlie the Witpoort Formation in the southern half of the study site. These shales are highly deformed and display closely spaced thrust faults and close folds. The study area encapsulates a range of folding from tight to open folds. Faulting consists of low angle north verging thrust fault, south verging back thrusts, south and north dipping normal faults, and strike-slip faults. Closely spaced, fore-land verging thrusts faults predominate over hinterland verging back thrusts. Normal faulting post-dates thrust faulting and utilized weaknesses in axial planar cleavage and in certain instances existing thrust fault planes. Strike-slip faulting post-dates thrusting and has in places reactivated pre-existing thrust fault planes. Macro scale folding includes overturned synclines and large anticlines which have been eroded, exposing older strata. Fold axes plunge at low to moderate angles west-southwest. This correlates with tension gashes which indicate north westward directed forces. Eastward directed forces are confirmed by the presence of tension gashes and strike-slip movement. The local geology displays north westward directed compression followed by strike-slip movement. Normal faulting post-dates all other structures and is associated with the Mesozoic break-up of Gondwana. The Perdepoort Member was sampled along strike, at different outcrop latitudes. Seven samples were selected for scanning electron microscope analysis. Samples are composed almost entirely of quartz; accessories include, biotite, muscovite, sericite, baryte, and apatite. Epigenetic hematite is present along cracks within certain samples Epigenetic hematite occur along cracks with oxides and phosphates in the form of rutile, apatite and monazite present in a number of samples. When compared to other silica extraction operations the Perdepoort Member appears viable for explotation. However, for the solar cell industry the purity of this horizon is clearly far below that required for industy.
- Full Text:
- Date Issued: 2010
The ichthyofauna and piscivorous avifauna in a small temporarily open/closed Eastern Cape estuary, South Africa
- Authors: Blake, Justin David
- Date: 2010
- Subjects: Estuarine fishes -- South Africa -- Eastern Cape , Bird populations -- South Africa -- Riet River Estuary , Estuaries -- South Africa -- Eastern Cape , Fish populations -- South Africa -- Riet River Estuary , Estuarine animals -- South Africa -- Riet River Estuary , Birds -- South Africa -- Riet River Estuary , Riet River Estuary (South Africa)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5709 , http://hdl.handle.net/10962/d1005395 , Estuarine fishes -- South Africa -- Eastern Cape , Bird populations -- South Africa -- Riet River Estuary , Estuaries -- South Africa -- Eastern Cape , Fish populations -- South Africa -- Riet River Estuary , Estuarine animals -- South Africa -- Riet River Estuary , Birds -- South Africa -- Riet River Estuary , Riet River Estuary (South Africa)
- Description: The spatial and temporal patterns in selected components of the ichthyofauna and piscivorous avifauna in the small temporarily open/closed Riet River Estuary located on the eastern seaboard of southern Africa was investigated monthly over the period August 2005 to July 2006. The ichthyofauna within the littoral zone of the estuary was sampled using a 5 m seine net (8 stations) while a 30 m seine net (4 stations) was employed to sample the fish in the channel. Bird counts were made along repeat transects along the length of the estuary. Total ichthyofaunal abundances and biomass ranged between 1.60 and 8.67 individuals m⁻² and 0.45 to 21.76 g wwt m⁻² within the littoral zone, and between 0.08 and 0.44 individuals m⁻² and 0.58 and 36.52 g wwt m⁻² in the channel of the estuary. The highest values were generally recorded during the summer months. Results of the numerical analysis indicated that the breaching events recorded over the study period did not lead to a common trend in the ichthyofaunal community. In the absence of a link to the marine environment, the ichthyofaunal community in the littoral zone was numerically dominated by the estuarine resident species, Gilchristella aestuaria and to a lesser extent by Glossogobius callidus, which collectively accounted for ca. 54% of the total ichthyofauna sampled. The establishment of a link to the marine environment coincided with increased numbers of marine breeding species including Atherina breviceps and Rhabdosargus holubi to total fish counts within the estuary. Hierarchical cluster analysis did not identify any spatial patterns in the community structure of the ichthyofauna in the littoral zone or channel zone of the estuary, which could likely be linked to the absence of any distinct horizontal patterns in salinity and temperature within the system. A total of thirteen piscivorous bird species was recorded over the study period. Of the recorded species, six species were wading piscivores, four species were aerial divers and the remaining three species were pursuit swimmers. There were no significant correlations between the estimates of the ichthyofaunal abundance and biomass and bird numbers evident during the study (P> 0.05 in both cases). The Reed Cormorant (Phalacrocorax africanus) was the dominant species throughout the study, with a mean of 8.25 (SD ± 7.90) individuals per count. Mean values of the Pied Kingfisher (Ceryle rudis) and Giant Kingfisher (Megaceryle maximus) were 3.42 (SD ± 1.20) and 1.17 (SD ± 0.60) individuals per count, respectively. The remaining species revealed mean values < 0.5 individuals per count. The highest bird numbers were recorded in winter reflecting the migration of large numbers of the Reed Cormorant into the system. Breaching events were associated with a decrease in total bird numbers, which was most likely due to loss of potential foraging habitat (littoral zone) for waders resulting from reduced water levels. Monthly food consumption by all piscivorous birds revealed large temporal variability, ranging from 26.35 to 140.58 kg per month. The observed variability could be linked to mouth phase and bird numbers.
- Full Text:
- Date Issued: 2010
- Authors: Blake, Justin David
- Date: 2010
- Subjects: Estuarine fishes -- South Africa -- Eastern Cape , Bird populations -- South Africa -- Riet River Estuary , Estuaries -- South Africa -- Eastern Cape , Fish populations -- South Africa -- Riet River Estuary , Estuarine animals -- South Africa -- Riet River Estuary , Birds -- South Africa -- Riet River Estuary , Riet River Estuary (South Africa)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5709 , http://hdl.handle.net/10962/d1005395 , Estuarine fishes -- South Africa -- Eastern Cape , Bird populations -- South Africa -- Riet River Estuary , Estuaries -- South Africa -- Eastern Cape , Fish populations -- South Africa -- Riet River Estuary , Estuarine animals -- South Africa -- Riet River Estuary , Birds -- South Africa -- Riet River Estuary , Riet River Estuary (South Africa)
- Description: The spatial and temporal patterns in selected components of the ichthyofauna and piscivorous avifauna in the small temporarily open/closed Riet River Estuary located on the eastern seaboard of southern Africa was investigated monthly over the period August 2005 to July 2006. The ichthyofauna within the littoral zone of the estuary was sampled using a 5 m seine net (8 stations) while a 30 m seine net (4 stations) was employed to sample the fish in the channel. Bird counts were made along repeat transects along the length of the estuary. Total ichthyofaunal abundances and biomass ranged between 1.60 and 8.67 individuals m⁻² and 0.45 to 21.76 g wwt m⁻² within the littoral zone, and between 0.08 and 0.44 individuals m⁻² and 0.58 and 36.52 g wwt m⁻² in the channel of the estuary. The highest values were generally recorded during the summer months. Results of the numerical analysis indicated that the breaching events recorded over the study period did not lead to a common trend in the ichthyofaunal community. In the absence of a link to the marine environment, the ichthyofaunal community in the littoral zone was numerically dominated by the estuarine resident species, Gilchristella aestuaria and to a lesser extent by Glossogobius callidus, which collectively accounted for ca. 54% of the total ichthyofauna sampled. The establishment of a link to the marine environment coincided with increased numbers of marine breeding species including Atherina breviceps and Rhabdosargus holubi to total fish counts within the estuary. Hierarchical cluster analysis did not identify any spatial patterns in the community structure of the ichthyofauna in the littoral zone or channel zone of the estuary, which could likely be linked to the absence of any distinct horizontal patterns in salinity and temperature within the system. A total of thirteen piscivorous bird species was recorded over the study period. Of the recorded species, six species were wading piscivores, four species were aerial divers and the remaining three species were pursuit swimmers. There were no significant correlations between the estimates of the ichthyofaunal abundance and biomass and bird numbers evident during the study (P> 0.05 in both cases). The Reed Cormorant (Phalacrocorax africanus) was the dominant species throughout the study, with a mean of 8.25 (SD ± 7.90) individuals per count. Mean values of the Pied Kingfisher (Ceryle rudis) and Giant Kingfisher (Megaceryle maximus) were 3.42 (SD ± 1.20) and 1.17 (SD ± 0.60) individuals per count, respectively. The remaining species revealed mean values < 0.5 individuals per count. The highest bird numbers were recorded in winter reflecting the migration of large numbers of the Reed Cormorant into the system. Breaching events were associated with a decrease in total bird numbers, which was most likely due to loss of potential foraging habitat (littoral zone) for waders resulting from reduced water levels. Monthly food consumption by all piscivorous birds revealed large temporal variability, ranging from 26.35 to 140.58 kg per month. The observed variability could be linked to mouth phase and bird numbers.
- Full Text:
- Date Issued: 2010
The impact of a one-hour self-selected nap opportunity on physiological and performance variables during a simulated night shift
- Authors: Davy, Jonathan Patrick
- Date: 2010
- Subjects: Night work , Naps (sleep) in the workplace , Naps (sleep) in the workplace -- Case studies , Shift systems
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5123 , http://hdl.handle.net/10962/d1005201 , Night work , Naps (sleep) in the workplace , Naps (sleep) in the workplace -- Case studies , Shift systems
- Description: Napping has been explored extensively as a means of counteracting the negative effects associated with shift work. A significant amount of this research has focused on the implementation of scheduled naps, with few studies considering flexible nap schemes. The current study therefore aimed to assess the effects of a flexible nap opportunity on the physiological, cognitive, performance, neurophysiological and subjective responses of a group of non shift workers over the course of a three-day simulated night shift regime. Additional foci were the effects of the nap condition on the extent of the circadian adaptation of the subjects to the irregular work schedule and the circadian-related influences associated with being awake during the night. 36 subjects – 18 males and 18 females – were recruited to participate in the current study. The data collection spanned twelve days, during which four, three-day long shift cycles were set up: three night shift cycles and one day shift cycle. During each night shift cycle, three separate experimental conditions were staggered, namely the nap condition, the no nap condition and a booster break condition (a collaborative study that completed the setup). The day shift served as a further comparison. Each cycle comprised of 12 subjects, which meant there were four subjects per condition during each cycle. The shifts were 8 hours in duration, with the no nap group following a standard break schedule evinced in industry. The three breaks taken during the shifts amounted to a total time of 1 hour. The nap group was afforded a 1 hour flexible nap opportunity between 00h00 and 03h00 with no other breaks. Therefore, both conditions had the same amount of work time. During the shifts, subjects performed two simple, low arousal tasks (beading and packing) and completed a test battery roughly every two hours which was comprised of physiological, performance, neurophysiological and subjective measures. It was found that the inclusion of the nap opportunity significantly improved output performance and response time during a low precision, modified Fitts tapping task over the course of three night shifts, relative to no napping. Physiologically, napping resulted in higher heart rate frequency measures by the end of the shifts, which were also accompanied by significant reductions in subjective sleepiness ratings during all iii the night shifts. The nap group’s responses in this case, did not differ significantly from those of the day shift. Both simple reaction time and memory performances improved as a result of the nap inclusion, but only during the third night shift. The majority of the measures included in the research also depicted the effects of the circadian rhythm, which was indicative of the pronounced effect that this natural biological down regulation has on performance during the night. Napping reduced the severity of these effects during beading performance and measures of subjective sleepiness. With regard to habituation, the nap opportunity also resulted in positive changes in the responses of beading performance, high precision response time, simple reaction time and both subjective sleepiness measures, relative to no napping. Sleep diary responses indicated that although sleep length and quality during the day were significantly reduced for both night-time conditions, recovery sleep (length and quality) for the nap group did not differ significantly from the no nap group. The findings of this research indicate that the inclusion of a flexible napping opportunity during the night shift had positive effects on some physiological, performance and subjective responses, and that this intervention is as beneficial as scheduled napping. Specifically, napping resulted in a significantly higher output during the beading task, relative to the no nap group despite the duration of work time being the same. As such the introduction of a flexible, self-selected nap opportunity is a practical, effective and individual-specific means of alleviating the negative effects of shift work, while improving certain performance parameters. Therefore, industries should consider its inclusion in their fatigue management programs. However, contextspecific considerations must be made, with regard work scheduling, individual differences and task demands when implementing such an intervention. This will ensure that its introduction will be well received and in time, lessen the health and work-related decrements associated with shift work.
- Full Text:
- Date Issued: 2010
- Authors: Davy, Jonathan Patrick
- Date: 2010
- Subjects: Night work , Naps (sleep) in the workplace , Naps (sleep) in the workplace -- Case studies , Shift systems
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5123 , http://hdl.handle.net/10962/d1005201 , Night work , Naps (sleep) in the workplace , Naps (sleep) in the workplace -- Case studies , Shift systems
- Description: Napping has been explored extensively as a means of counteracting the negative effects associated with shift work. A significant amount of this research has focused on the implementation of scheduled naps, with few studies considering flexible nap schemes. The current study therefore aimed to assess the effects of a flexible nap opportunity on the physiological, cognitive, performance, neurophysiological and subjective responses of a group of non shift workers over the course of a three-day simulated night shift regime. Additional foci were the effects of the nap condition on the extent of the circadian adaptation of the subjects to the irregular work schedule and the circadian-related influences associated with being awake during the night. 36 subjects – 18 males and 18 females – were recruited to participate in the current study. The data collection spanned twelve days, during which four, three-day long shift cycles were set up: three night shift cycles and one day shift cycle. During each night shift cycle, three separate experimental conditions were staggered, namely the nap condition, the no nap condition and a booster break condition (a collaborative study that completed the setup). The day shift served as a further comparison. Each cycle comprised of 12 subjects, which meant there were four subjects per condition during each cycle. The shifts were 8 hours in duration, with the no nap group following a standard break schedule evinced in industry. The three breaks taken during the shifts amounted to a total time of 1 hour. The nap group was afforded a 1 hour flexible nap opportunity between 00h00 and 03h00 with no other breaks. Therefore, both conditions had the same amount of work time. During the shifts, subjects performed two simple, low arousal tasks (beading and packing) and completed a test battery roughly every two hours which was comprised of physiological, performance, neurophysiological and subjective measures. It was found that the inclusion of the nap opportunity significantly improved output performance and response time during a low precision, modified Fitts tapping task over the course of three night shifts, relative to no napping. Physiologically, napping resulted in higher heart rate frequency measures by the end of the shifts, which were also accompanied by significant reductions in subjective sleepiness ratings during all iii the night shifts. The nap group’s responses in this case, did not differ significantly from those of the day shift. Both simple reaction time and memory performances improved as a result of the nap inclusion, but only during the third night shift. The majority of the measures included in the research also depicted the effects of the circadian rhythm, which was indicative of the pronounced effect that this natural biological down regulation has on performance during the night. Napping reduced the severity of these effects during beading performance and measures of subjective sleepiness. With regard to habituation, the nap opportunity also resulted in positive changes in the responses of beading performance, high precision response time, simple reaction time and both subjective sleepiness measures, relative to no napping. Sleep diary responses indicated that although sleep length and quality during the day were significantly reduced for both night-time conditions, recovery sleep (length and quality) for the nap group did not differ significantly from the no nap group. The findings of this research indicate that the inclusion of a flexible napping opportunity during the night shift had positive effects on some physiological, performance and subjective responses, and that this intervention is as beneficial as scheduled napping. Specifically, napping resulted in a significantly higher output during the beading task, relative to the no nap group despite the duration of work time being the same. As such the introduction of a flexible, self-selected nap opportunity is a practical, effective and individual-specific means of alleviating the negative effects of shift work, while improving certain performance parameters. Therefore, industries should consider its inclusion in their fatigue management programs. However, contextspecific considerations must be made, with regard work scheduling, individual differences and task demands when implementing such an intervention. This will ensure that its introduction will be well received and in time, lessen the health and work-related decrements associated with shift work.
- Full Text:
- Date Issued: 2010
The importance of estuarine head waters for fishes in selected Eastern Cape systems, with particular emphasis on the influence of freshwater inflow, migration barriers and non-native predators on the juvenile and small fish component
- Authors: Wasserman, Ryan
- Date: 2010
- Subjects: Estuaries -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10685 , http://hdl.handle.net/10948/1457 , Estuaries -- South Africa -- Eastern Cape
- Description: The utilisation of estuary headwater environments by young estuary- and marine-spawned fish species was investigated together with the effects of riverflow alteration, in-stream barrier effects and non-native ichthyofauna on the nursery function of these habitats. The distribution and abundance of young estuary- and marine-spawned fish were sampled using seine and fyke nets in the headwater environments of four permanently open Eastern Cape systems, namely the Great Fish, Kowie, Kariega and Sundays Estuaries. Within the suite of study systems, the first of two case studies focussed on barrier effects of in-stream structures on fish migration. This was undertaken in the Sundays River. In the second case study, predation and competition dynamics of the non-native piscivorous Micropterus salmoides on estuary-dependent fish was investigated in the estuary headwater regions of the Kowie River system. In all four estuaries, young estuary-spawned fish species dominated the ichthyofaunal community followed by marine-spawned species, despite varied freshwater inflow resulting in headwaters varying in salinity from fresh to hypersaline. Fish community structure however, differed largely between estuaries, with both freshwater abstraction and unnatural elevation of freshwater into estuaries, as a result of inter-basin transfers, affecting these communities. In-stream structures were found to effect upstream movement of fish in two ways, dependent on the type of barrier. Partial (size-dependent) and complete (species-dependent) restriction to upstream migration of fish by causeway-type instream structures were observed. Weir-type in-stream structures acted as a complete barrier to most species, regardless of fish size. Predation of estuary- and marine-spawned fish species by large sized M. salmoides was recorded, although these fish did not contribute significantly to their diet during this study. However, the main dietary components found in smaller sized M. salmoides stomachs overlap with those of juvenile estuary- and marinespawned fish species, suggesting feeding competition between the juveniles of indigenous and non-native fish species.
- Full Text:
- Date Issued: 2010
- Authors: Wasserman, Ryan
- Date: 2010
- Subjects: Estuaries -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10685 , http://hdl.handle.net/10948/1457 , Estuaries -- South Africa -- Eastern Cape
- Description: The utilisation of estuary headwater environments by young estuary- and marine-spawned fish species was investigated together with the effects of riverflow alteration, in-stream barrier effects and non-native ichthyofauna on the nursery function of these habitats. The distribution and abundance of young estuary- and marine-spawned fish were sampled using seine and fyke nets in the headwater environments of four permanently open Eastern Cape systems, namely the Great Fish, Kowie, Kariega and Sundays Estuaries. Within the suite of study systems, the first of two case studies focussed on barrier effects of in-stream structures on fish migration. This was undertaken in the Sundays River. In the second case study, predation and competition dynamics of the non-native piscivorous Micropterus salmoides on estuary-dependent fish was investigated in the estuary headwater regions of the Kowie River system. In all four estuaries, young estuary-spawned fish species dominated the ichthyofaunal community followed by marine-spawned species, despite varied freshwater inflow resulting in headwaters varying in salinity from fresh to hypersaline. Fish community structure however, differed largely between estuaries, with both freshwater abstraction and unnatural elevation of freshwater into estuaries, as a result of inter-basin transfers, affecting these communities. In-stream structures were found to effect upstream movement of fish in two ways, dependent on the type of barrier. Partial (size-dependent) and complete (species-dependent) restriction to upstream migration of fish by causeway-type instream structures were observed. Weir-type in-stream structures acted as a complete barrier to most species, regardless of fish size. Predation of estuary- and marine-spawned fish species by large sized M. salmoides was recorded, although these fish did not contribute significantly to their diet during this study. However, the main dietary components found in smaller sized M. salmoides stomachs overlap with those of juvenile estuary- and marinespawned fish species, suggesting feeding competition between the juveniles of indigenous and non-native fish species.
- Full Text:
- Date Issued: 2010