Assessing Nelson Mandela Bay Municipality's urban resilience
- Authors: Gatang'i, Rebecca Nyangige
- Date: 2016
- Subjects: Sustainable development -- South Africa -- Nelson Mandela Bay Municipality , City planning -- South Africa -- Nelson Mandela Bay Municipality
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/7637 , vital:22273
- Description: The quest for resilient cities has emerged as a strategy to mitigate and adapt the problems created by urban population growth and rapid urbanisation. Resilience has increasingly become an important urban policy discourse that challenge cities to reflect on their adaptive capacity to function in the face of adversity. Urban resilience in particular, amplifies the concern that urban spaces are the hub of heightened complexities of diverse risks. Across the globe, the concept of resilience is gaining momentum with many academic researchers discussing this phenomenon. In South Africa, the concept has been sparingly incorporated in a number of literatures with little focus on local government entities such as municipalities. In order to close this gap in the literature, and in response to the increasing use of the concept locally, this study explores the extent to which factors of resilience resonate within the Nelson Mandela Bay Municipality urban management practices. Based on the exploration of a wide array of literature from various disciplinary areas, this study examines the concept of urbanisation and the related challenges. It also critically explores the concept of resilience, its application in urban management as well as the attributes of the adaptive capacities that enable urban resilience. This research adopts the City Resilience Framework and Index developed by Arup in partnership with the Rockefeller Foundation to assess the levels of resilience in the Nelson Mandela Bay Municipality. The framework identifies four main dimensions used to measure resilience: People’s health and wellbeing, Organisation of the economy and society, Place of urban systems and services and Knowledge inherent in leadership and strategy. These dimensions form the basis of an integrative framework that provides an alternative lense through which cities can understand their unique attributes that contribute to their resilience. This research highlights the levels of resilience within the Nelson Mandela Bay Municipality and outlines practical implications for Nelson Mandela Bay Municipality’s management which can also be applied in other cities. This research adopts a positivist approach and applies statistical empirical measures using a quantitative analysis process. The research instrument in the form of a questionnaire was administered to the target population for data collection. Using the conceptual framework, the researcher applied statistical analysis to derive relationships amongst the variables to determine the degree of resilience in Nelson Mandela Bay Municipality using the four main dimensions of the framework. The findings of the study show the varying levels of resilience within NMBM’s practices. NMBM’s principal success areas include; providing basic houses, ensuring water and sanitation facilities, instituting effective labour policies, providing skills development and training facilities especially to the youth, enabling a conducive atmosphere for business development and the presence of an integrated development strategy. However, not much has been done in providing sufficient public health facilities, alternative energy sources, access to financial services, ensuring food security, enabling continuity of projects and activities through a funded budget, providing emergency medical services in the event of a disaster, high levels of corruption and insufficient deterrents to crime. Overall, the results show that NMBM has made good strides in enabling high level of resilience in its day to day operations. The findings of this study suggest that urban resilience is a continuous process that facilitates engagement leading to a dialogue and deeper understanding of a city.
- Full Text:
- Date Issued: 2016
- Authors: Gatang'i, Rebecca Nyangige
- Date: 2016
- Subjects: Sustainable development -- South Africa -- Nelson Mandela Bay Municipality , City planning -- South Africa -- Nelson Mandela Bay Municipality
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/7637 , vital:22273
- Description: The quest for resilient cities has emerged as a strategy to mitigate and adapt the problems created by urban population growth and rapid urbanisation. Resilience has increasingly become an important urban policy discourse that challenge cities to reflect on their adaptive capacity to function in the face of adversity. Urban resilience in particular, amplifies the concern that urban spaces are the hub of heightened complexities of diverse risks. Across the globe, the concept of resilience is gaining momentum with many academic researchers discussing this phenomenon. In South Africa, the concept has been sparingly incorporated in a number of literatures with little focus on local government entities such as municipalities. In order to close this gap in the literature, and in response to the increasing use of the concept locally, this study explores the extent to which factors of resilience resonate within the Nelson Mandela Bay Municipality urban management practices. Based on the exploration of a wide array of literature from various disciplinary areas, this study examines the concept of urbanisation and the related challenges. It also critically explores the concept of resilience, its application in urban management as well as the attributes of the adaptive capacities that enable urban resilience. This research adopts the City Resilience Framework and Index developed by Arup in partnership with the Rockefeller Foundation to assess the levels of resilience in the Nelson Mandela Bay Municipality. The framework identifies four main dimensions used to measure resilience: People’s health and wellbeing, Organisation of the economy and society, Place of urban systems and services and Knowledge inherent in leadership and strategy. These dimensions form the basis of an integrative framework that provides an alternative lense through which cities can understand their unique attributes that contribute to their resilience. This research highlights the levels of resilience within the Nelson Mandela Bay Municipality and outlines practical implications for Nelson Mandela Bay Municipality’s management which can also be applied in other cities. This research adopts a positivist approach and applies statistical empirical measures using a quantitative analysis process. The research instrument in the form of a questionnaire was administered to the target population for data collection. Using the conceptual framework, the researcher applied statistical analysis to derive relationships amongst the variables to determine the degree of resilience in Nelson Mandela Bay Municipality using the four main dimensions of the framework. The findings of the study show the varying levels of resilience within NMBM’s practices. NMBM’s principal success areas include; providing basic houses, ensuring water and sanitation facilities, instituting effective labour policies, providing skills development and training facilities especially to the youth, enabling a conducive atmosphere for business development and the presence of an integrated development strategy. However, not much has been done in providing sufficient public health facilities, alternative energy sources, access to financial services, ensuring food security, enabling continuity of projects and activities through a funded budget, providing emergency medical services in the event of a disaster, high levels of corruption and insufficient deterrents to crime. Overall, the results show that NMBM has made good strides in enabling high level of resilience in its day to day operations. The findings of this study suggest that urban resilience is a continuous process that facilitates engagement leading to a dialogue and deeper understanding of a city.
- Full Text:
- Date Issued: 2016
Assessing Nelson Mandela Bay Municipality's urban resilience
- Authors: Gatang'i, Rebecca Nyangige
- Date: 2016
- Subjects: City planning -- South Africa -- Nelson Mandela Bay Municipality , Sustainable development -- South Africa -- Nelson Mandela Bay Municipality
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/7639 , vital:22272
- Description: The quest for resilient cities has emerged as a strategy to mitigate and adapt the problems created by urban population growth and rapid urbanisation. Resilience has increasingly become an important urban policy discourse that challenge cities to reflect on their adaptive capacity to function in the face of adversity. Urban resilience in particular, amplifies the concern that urban spaces are the hub of heightened complexities of diverse risks. Across the globe, the concept of resilience is gaining momentum with many academic researchers discussing this phenomenon. In South Africa, the concept has been sparingly incorporated in a number of literatures with little focus on local government entities such as municipalities. In order to close this gap in the literature, and in response to the increasing use of the concept locally, this study explores the extent to which factors of resilience resonate within the Nelson Mandela Bay Municipality urban management practices. Based on the exploration of a wide array of literature from various disciplinary areas, this study examines the concept of urbanisation and the related challenges. It also critically explores the concept of resilience, its application in urban management as well as the attributes of the adaptive capacities that enable urban resilience. This research adopts the City Resilience Framework and Index developed by Arup in partnership with the Rockefeller Foundation to assess the levels of resilience in the Nelson Mandela Bay Municipality. The framework identifies four main dimensions used to measure resilience: People’s health and wellbeing, Organisation of the economy and society, Place of urban systems and services and Knowledge inherent in leadership and strategy. These dimensions form the basis of an integrative framework that provides an alternative lense through which cities can understand their unique attributes that contribute to their resilience. This research highlights the levels of resilience within the Nelson Mandela Bay Municipality and outlines practical implications for Nelson Mandela Bay Municipality’s management which can also be applied in other cities. This research adopts a positivist approach and applies statistical empirical measures using a quantitative analysis process. The research instrument in the form of a questionnaire was administered to the target population for data collection. Using the conceptual framework, the researcher applied statistical analysis to derive relationships amongst the variables to determine the degree of resilience in Nelson Mandela Bay Municipality using the four main dimensions of the framework. The findings of the study show the varying levels of resilience within NMBM’s practices. NMBM’s principal success areas include; providing basic houses, ensuring water and sanitation facilities, instituting effective labour policies, providing skills development and training facilities especially to the youth, enabling a conducive atmosphere for business development and the presence of an integrated development strategy. However, not much has been done in providing sufficient public health facilities, alternative energy sources, access to financial services, ensuring food security, enabling continuity of projects and activities through a funded budget, providing emergency medical services in the event of a disaster, high levels of corruption and insufficient deterrents to crime. Overall, the results show that NMBM has made good strides in enabling high level of resilience in its day to day operations. The findings of this study suggest that urban resilience is a continuous process that facilitates engagement leading to a dialogue and deeper understanding of a city.
- Full Text:
- Date Issued: 2016
- Authors: Gatang'i, Rebecca Nyangige
- Date: 2016
- Subjects: City planning -- South Africa -- Nelson Mandela Bay Municipality , Sustainable development -- South Africa -- Nelson Mandela Bay Municipality
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/7639 , vital:22272
- Description: The quest for resilient cities has emerged as a strategy to mitigate and adapt the problems created by urban population growth and rapid urbanisation. Resilience has increasingly become an important urban policy discourse that challenge cities to reflect on their adaptive capacity to function in the face of adversity. Urban resilience in particular, amplifies the concern that urban spaces are the hub of heightened complexities of diverse risks. Across the globe, the concept of resilience is gaining momentum with many academic researchers discussing this phenomenon. In South Africa, the concept has been sparingly incorporated in a number of literatures with little focus on local government entities such as municipalities. In order to close this gap in the literature, and in response to the increasing use of the concept locally, this study explores the extent to which factors of resilience resonate within the Nelson Mandela Bay Municipality urban management practices. Based on the exploration of a wide array of literature from various disciplinary areas, this study examines the concept of urbanisation and the related challenges. It also critically explores the concept of resilience, its application in urban management as well as the attributes of the adaptive capacities that enable urban resilience. This research adopts the City Resilience Framework and Index developed by Arup in partnership with the Rockefeller Foundation to assess the levels of resilience in the Nelson Mandela Bay Municipality. The framework identifies four main dimensions used to measure resilience: People’s health and wellbeing, Organisation of the economy and society, Place of urban systems and services and Knowledge inherent in leadership and strategy. These dimensions form the basis of an integrative framework that provides an alternative lense through which cities can understand their unique attributes that contribute to their resilience. This research highlights the levels of resilience within the Nelson Mandela Bay Municipality and outlines practical implications for Nelson Mandela Bay Municipality’s management which can also be applied in other cities. This research adopts a positivist approach and applies statistical empirical measures using a quantitative analysis process. The research instrument in the form of a questionnaire was administered to the target population for data collection. Using the conceptual framework, the researcher applied statistical analysis to derive relationships amongst the variables to determine the degree of resilience in Nelson Mandela Bay Municipality using the four main dimensions of the framework. The findings of the study show the varying levels of resilience within NMBM’s practices. NMBM’s principal success areas include; providing basic houses, ensuring water and sanitation facilities, instituting effective labour policies, providing skills development and training facilities especially to the youth, enabling a conducive atmosphere for business development and the presence of an integrated development strategy. However, not much has been done in providing sufficient public health facilities, alternative energy sources, access to financial services, ensuring food security, enabling continuity of projects and activities through a funded budget, providing emergency medical services in the event of a disaster, high levels of corruption and insufficient deterrents to crime. Overall, the results show that NMBM has made good strides in enabling high level of resilience in its day to day operations. The findings of this study suggest that urban resilience is a continuous process that facilitates engagement leading to a dialogue and deeper understanding of a city.
- Full Text:
- Date Issued: 2016
Assessing the effect of absenteeism in the Eastern Cape Department of Health
- Authors: Simon, Nobuzwe
- Date: 2016
- Subjects: Absenteeism (Labor) , Public health personnel , Absenteeism (Labor) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10353/2271 , vital:27736
- Description: The Department of Health is experiencing repeated negative press releases with the respect to the quality of service delivery in the Eastern Cape. The public sector is an entity that provides a unique service. This is made evident by the services provided such as provision of free health services for primary health care as well as free health services to children under six, disabled and senior citizens. The Eastern Cape Department of Health has developed service standards adopted in 2007 by the Eastern Cape Department of Health. This study aims to determine the casual effect of absenteeism and their relationship to poor service delivery. According to Makhubu (2006) many vacancies in the Eastern Cape Department of Health exist which result in service delivery problems. In terms of the second principle in the Batho Pele Principles the norms and standards which are service standards serves as a baseline where the Department of Public Service and Administration (2007) issued directive that the vacancy rate of any government department should range between ten and fifteen percent Makhubu (2006) further refers to the doctors/patient ratio as one is to three hundred patients (1:300), which is unhealthy and abnormal as the doctor/patient ration in terms of the Health Professional is one is to thirty (1:30). Such cases pose an opportunity to those employees who are within the service to absent themselves from such unhealthy situations and this behavior later result in the exodus (professional drainage) of employee. Schultz, Nel, Gerber, Hassbroek, Van Dyk and Werner (2001:582) define absenteeism as withdrawal of levels of productivity and collegial interaction for a given time to escape a perceived undesirable working environment. This behavior is disruptive to continuous organizational efficiency. Absenteeism is found in two forms that are avoidable and unavoidable. In the unavoidable forms of absenteeism, the situation in unplanned and is governed by external factors, thus it is beyond one’s control and has to be managed differently. Control of absenteeism can exercise by the immediate manager through the human resource section regarding the duration and terms of absence pertaining salary and terms of services.
- Full Text:
- Date Issued: 2016
- Authors: Simon, Nobuzwe
- Date: 2016
- Subjects: Absenteeism (Labor) , Public health personnel , Absenteeism (Labor) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10353/2271 , vital:27736
- Description: The Department of Health is experiencing repeated negative press releases with the respect to the quality of service delivery in the Eastern Cape. The public sector is an entity that provides a unique service. This is made evident by the services provided such as provision of free health services for primary health care as well as free health services to children under six, disabled and senior citizens. The Eastern Cape Department of Health has developed service standards adopted in 2007 by the Eastern Cape Department of Health. This study aims to determine the casual effect of absenteeism and their relationship to poor service delivery. According to Makhubu (2006) many vacancies in the Eastern Cape Department of Health exist which result in service delivery problems. In terms of the second principle in the Batho Pele Principles the norms and standards which are service standards serves as a baseline where the Department of Public Service and Administration (2007) issued directive that the vacancy rate of any government department should range between ten and fifteen percent Makhubu (2006) further refers to the doctors/patient ratio as one is to three hundred patients (1:300), which is unhealthy and abnormal as the doctor/patient ration in terms of the Health Professional is one is to thirty (1:30). Such cases pose an opportunity to those employees who are within the service to absent themselves from such unhealthy situations and this behavior later result in the exodus (professional drainage) of employee. Schultz, Nel, Gerber, Hassbroek, Van Dyk and Werner (2001:582) define absenteeism as withdrawal of levels of productivity and collegial interaction for a given time to escape a perceived undesirable working environment. This behavior is disruptive to continuous organizational efficiency. Absenteeism is found in two forms that are avoidable and unavoidable. In the unavoidable forms of absenteeism, the situation in unplanned and is governed by external factors, thus it is beyond one’s control and has to be managed differently. Control of absenteeism can exercise by the immediate manager through the human resource section regarding the duration and terms of absence pertaining salary and terms of services.
- Full Text:
- Date Issued: 2016
Assessing the impacts of social grants on beneficiaries in Buffalo City Municipality
- Authors: Dodo, Emmanuel Oluwafemi
- Date: 2016
- Subjects: Social security -- South Africa -- Eastern Cape Grants-in-aid -- South Africa -- Eastern Cape Poor -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , Degree
- Identifier: http://hdl.handle.net/10353/2967 , vital:28206
- Description: The study was intended to assess the impact of social grants on beneficiaries with specific focus on Buffalo Municipality. The challenge of social injustice and inequality in the management and distribution of resources is challenge of any responsive government globally in providing social securities in different forms to secure the vulnerable and under privileged within the society. The South African government has introduced a comprehensive liberally inclusive social welfare scheme to address social injustice and economic inequality of excluded in the former apartheid government, which characterises the historic past of racial exclusive government of the majority black and the coloured. In other to assess the impact of grants in addressing inequality and social exclusiveness, the research was conducted using Desk Top Analytical approach and also explored ANOVA Two-ways and One-way, to test the degree of association and standard deviation to establish the contrast of effects based on spatial difference and social development of the urban, semi-urban and rural settlements which beneficiaries are living. Also, to establish whether or not the policy is achieving social cohesion, human development and transformation, which should be taken, place within the concept of social welfare. The theoretical frame work adopted is a multi-theoretical approach in dealing with the complexity around social welfare. The research is specifically looking for a theory of change in social grants administration in other to understand the impact and extent of the intervention in bringing about desired and motivated change as the scheme main priority of developmental social welfare in South Africa. Pearson correlation, Chi-square correlation and Tukey”s Post hoc Test are some of the instrument used in assessing the impact of grants on beneficiaries. The outcome of the study showed a highly significance p-value =.019, p<0.05 in spatial variance between Urban and Rural and the in between variance of .025, p<0.05 for Rural –Urban- Semi-urban (Fig.23 and Tab 8a). The study also, revealed an association between Child dependency and Sexual crime to be .009, p<0.01 (Fig 24 and Tab 6), Public violence in Rural area shows Post Hoc Test result of Mean= 2.6, SD= 2.07 for rural, Mean=1.40 SD= 1.52, for urban and a Mean=0.3, SD=0.54 for semi-urban in a descending order as it relate to social crime statistics (Fig.20). This implies that there are more frequent public violence in rural areas than that of the urban and semi-urban. The overall result shows a very strong correlation among all the variables of grants beneficiaries to be statistically relevant. This implies that the social grants intervention.
- Full Text:
- Date Issued: 2016
- Authors: Dodo, Emmanuel Oluwafemi
- Date: 2016
- Subjects: Social security -- South Africa -- Eastern Cape Grants-in-aid -- South Africa -- Eastern Cape Poor -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , Degree
- Identifier: http://hdl.handle.net/10353/2967 , vital:28206
- Description: The study was intended to assess the impact of social grants on beneficiaries with specific focus on Buffalo Municipality. The challenge of social injustice and inequality in the management and distribution of resources is challenge of any responsive government globally in providing social securities in different forms to secure the vulnerable and under privileged within the society. The South African government has introduced a comprehensive liberally inclusive social welfare scheme to address social injustice and economic inequality of excluded in the former apartheid government, which characterises the historic past of racial exclusive government of the majority black and the coloured. In other to assess the impact of grants in addressing inequality and social exclusiveness, the research was conducted using Desk Top Analytical approach and also explored ANOVA Two-ways and One-way, to test the degree of association and standard deviation to establish the contrast of effects based on spatial difference and social development of the urban, semi-urban and rural settlements which beneficiaries are living. Also, to establish whether or not the policy is achieving social cohesion, human development and transformation, which should be taken, place within the concept of social welfare. The theoretical frame work adopted is a multi-theoretical approach in dealing with the complexity around social welfare. The research is specifically looking for a theory of change in social grants administration in other to understand the impact and extent of the intervention in bringing about desired and motivated change as the scheme main priority of developmental social welfare in South Africa. Pearson correlation, Chi-square correlation and Tukey”s Post hoc Test are some of the instrument used in assessing the impact of grants on beneficiaries. The outcome of the study showed a highly significance p-value =.019, p<0.05 in spatial variance between Urban and Rural and the in between variance of .025, p<0.05 for Rural –Urban- Semi-urban (Fig.23 and Tab 8a). The study also, revealed an association between Child dependency and Sexual crime to be .009, p<0.01 (Fig 24 and Tab 6), Public violence in Rural area shows Post Hoc Test result of Mean= 2.6, SD= 2.07 for rural, Mean=1.40 SD= 1.52, for urban and a Mean=0.3, SD=0.54 for semi-urban in a descending order as it relate to social crime statistics (Fig.20). This implies that there are more frequent public violence in rural areas than that of the urban and semi-urban. The overall result shows a very strong correlation among all the variables of grants beneficiaries to be statistically relevant. This implies that the social grants intervention.
- Full Text:
- Date Issued: 2016
Assessing the productivity of selective container terminals in Africa using Data Envelopment Analysis (DEA)
- Mienie, Barend Jacobus, Brettenny, Warren
- Authors: Mienie, Barend Jacobus , Brettenny, Warren
- Date: 2016
- Subjects: Data envelopment analysis -- Africa Employees -- Rating of -- Africa
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10948/12054 , vital:27026
- Description: Data envelopment analysis (DEA) is used to assess the efficiency of 15 container terminals in Africa. The models proposed by Charnes, Cooper and Rhodes (1978) and Banker, Charnes and Cooper (1984) are used to determine and rank the efficiencies of the container terminals for 2013 and 2014. The results show that selected South African container terminals can improve on their operations relative to some of their neighbours to the North. Bootstrapping methods are used to investigate and clarify the results. The Malmquist Productivity Index (MPI) model is used to track and explain changes in efficiency over the period of assessment.
- Full Text:
- Date Issued: 2016
- Authors: Mienie, Barend Jacobus , Brettenny, Warren
- Date: 2016
- Subjects: Data envelopment analysis -- Africa Employees -- Rating of -- Africa
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10948/12054 , vital:27026
- Description: Data envelopment analysis (DEA) is used to assess the efficiency of 15 container terminals in Africa. The models proposed by Charnes, Cooper and Rhodes (1978) and Banker, Charnes and Cooper (1984) are used to determine and rank the efficiencies of the container terminals for 2013 and 2014. The results show that selected South African container terminals can improve on their operations relative to some of their neighbours to the North. Bootstrapping methods are used to investigate and clarify the results. The Malmquist Productivity Index (MPI) model is used to track and explain changes in efficiency over the period of assessment.
- Full Text:
- Date Issued: 2016
Assessing the value of public investment into biological control research for invasive alien plants : the ARC PPRI Weeds Research Division
- Authors: Scarr, Lowell Martin
- Date: 2016
- Subjects: Alien plants -- Biological control , Invasive plants -- Biological control , Alien plants -- Economic aspects , Invasive plants -- Economic aspects , Weeds -- Biological control
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1126 , http://hdl.handle.net/10962/d1020604
- Description: This study investigates the economic impact of the ARC PPRI Weeds Research Division. The Division researches appropriate methods of biological control for invasive alien plants (IAPs). These plants pose an increasing threat to environmental integrity and ecosystem service provision impacting on economic potential. Since the work of the Division is considered a public good, a predominantly descriptive approach has been adopted for the valuation process. A combination of quantitative cost analysis and a qualitative study of the impacts of research and invasive alien plants is used to deal with the challenges associated with non-market valuation. The study found that investment into the Weeds Division is a valuable activity that supports the long-term growth potential of the South African economy. The role of a well-functioning environment is highlighted as an essential base for the creation of sustained growth opportunities in any society. It was determined that investment into the Division should be increased into the future to support efficient spending of scarce state funds. Biological control research was found to provide strategic future growth potential, creating opportunities for the development of a competitive advantage in the biotechnology and environmental management sectors. The study adds to the increasing move towards a more holistic view of economic valuation, taking factors other than pure finance and econometrics into consideration. This is an important shift in prevailing economic thought, as a realisation is reached that a single, or even triple, bottom line is an outdated and insufficient decision making basis.
- Full Text:
- Date Issued: 2016
- Authors: Scarr, Lowell Martin
- Date: 2016
- Subjects: Alien plants -- Biological control , Invasive plants -- Biological control , Alien plants -- Economic aspects , Invasive plants -- Economic aspects , Weeds -- Biological control
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1126 , http://hdl.handle.net/10962/d1020604
- Description: This study investigates the economic impact of the ARC PPRI Weeds Research Division. The Division researches appropriate methods of biological control for invasive alien plants (IAPs). These plants pose an increasing threat to environmental integrity and ecosystem service provision impacting on economic potential. Since the work of the Division is considered a public good, a predominantly descriptive approach has been adopted for the valuation process. A combination of quantitative cost analysis and a qualitative study of the impacts of research and invasive alien plants is used to deal with the challenges associated with non-market valuation. The study found that investment into the Weeds Division is a valuable activity that supports the long-term growth potential of the South African economy. The role of a well-functioning environment is highlighted as an essential base for the creation of sustained growth opportunities in any society. It was determined that investment into the Division should be increased into the future to support efficient spending of scarce state funds. Biological control research was found to provide strategic future growth potential, creating opportunities for the development of a competitive advantage in the biotechnology and environmental management sectors. The study adds to the increasing move towards a more holistic view of economic valuation, taking factors other than pure finance and econometrics into consideration. This is an important shift in prevailing economic thought, as a realisation is reached that a single, or even triple, bottom line is an outdated and insufficient decision making basis.
- Full Text:
- Date Issued: 2016
Assessing the vulnerability of resource-poor households to disasters associated with climate variability using remote sensing and GIS techniques in the Nkonkobe Local Municipality, Eastern Cape Province, South Africa
- Authors: Chari, Martin Munashe
- Date: 2016
- Subjects: Climatic changes -- Forecasting Natural resources -- Remote sensing Droughts -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2425 , vital:27837
- Description: The main objective of the study was to assess the extent to which resource-poor households in selected villages of Nkonkobe Local Municipality in the Eastern Cape Province of South Africa are vulnerable to drought by using an improvised remote sensing and Geographic Information System (GIS)-based mapping approach. The research methodology was comprised of 1) assessment of vulnerability levels and 2) the calculation of established drought assessment indices comprising the Normalized Difference Vegetation Index (NDVI) and the Normalized Difference Water Index (NDWI) from wet-season Landsat images covering a period of 29 years from 1985 to 2014 in order to objectively determine the temporal recurrence of drought in Nkonkobe Local Municipality. Vulnerability of households to drought was determined by using a multi-step GIS-based mapping approach in which 3 components comprising exposure, sensitivity and adaptive capacity were simultaneously analysed and averaged to determine the magnitude of vulnerability. Thereafter, the Analytical Hierarchy Process (AHP) was used to establish weighted contributions of these components to vulnerability. The weights applied to the AHP were obtained from the 2012 - 2017 Nkonkobe Integrated Development Plan (IDP) and perceptions that were solicited from key informants who were judged to be knowledgeable about the subject. A Kruskal-Wallis H test on demographic data for water access revealed that the demographic results are independent of choice of data acquired from different data providers (χ2(2) = 1.26, p = 0.533, with a mean ranked population scores of 7.4 for ECSECC, 6.8 for Quantec and 9.8 for StatsSA). Simple linear regression analysis revealed strong positive correlations between NDWI and NDVI ((r = 0.99609375, R2 = 1, for 1985), 1995 (r = 0.99609375, R2 = 1 for 1995), (r = 0.99609375, R2 = 1 for 2005) and (r = 0.99609375, R2 = 1 for 2014). The regression analysis proved that vegetation condition depends on surface water arising from rainfall. The results indicate that the whole of Nkonkobe Local Municipality is susceptible to drought with villages in south eastern part being most vulnerable to droughts due to high sensitivity and low adaptive capacity.
- Full Text:
- Date Issued: 2016
- Authors: Chari, Martin Munashe
- Date: 2016
- Subjects: Climatic changes -- Forecasting Natural resources -- Remote sensing Droughts -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2425 , vital:27837
- Description: The main objective of the study was to assess the extent to which resource-poor households in selected villages of Nkonkobe Local Municipality in the Eastern Cape Province of South Africa are vulnerable to drought by using an improvised remote sensing and Geographic Information System (GIS)-based mapping approach. The research methodology was comprised of 1) assessment of vulnerability levels and 2) the calculation of established drought assessment indices comprising the Normalized Difference Vegetation Index (NDVI) and the Normalized Difference Water Index (NDWI) from wet-season Landsat images covering a period of 29 years from 1985 to 2014 in order to objectively determine the temporal recurrence of drought in Nkonkobe Local Municipality. Vulnerability of households to drought was determined by using a multi-step GIS-based mapping approach in which 3 components comprising exposure, sensitivity and adaptive capacity were simultaneously analysed and averaged to determine the magnitude of vulnerability. Thereafter, the Analytical Hierarchy Process (AHP) was used to establish weighted contributions of these components to vulnerability. The weights applied to the AHP were obtained from the 2012 - 2017 Nkonkobe Integrated Development Plan (IDP) and perceptions that were solicited from key informants who were judged to be knowledgeable about the subject. A Kruskal-Wallis H test on demographic data for water access revealed that the demographic results are independent of choice of data acquired from different data providers (χ2(2) = 1.26, p = 0.533, with a mean ranked population scores of 7.4 for ECSECC, 6.8 for Quantec and 9.8 for StatsSA). Simple linear regression analysis revealed strong positive correlations between NDWI and NDVI ((r = 0.99609375, R2 = 1, for 1985), 1995 (r = 0.99609375, R2 = 1 for 1995), (r = 0.99609375, R2 = 1 for 2005) and (r = 0.99609375, R2 = 1 for 2014). The regression analysis proved that vegetation condition depends on surface water arising from rainfall. The results indicate that the whole of Nkonkobe Local Municipality is susceptible to drought with villages in south eastern part being most vulnerable to droughts due to high sensitivity and low adaptive capacity.
- Full Text:
- Date Issued: 2016
Assessment of government spending austerity measures in on-site school support for curriculum delivery: a case of Idutywa Education District
- Authors: Lombo, Nomachule
- Date: 2016
- Subjects: Government spending policy -- South Africa -- Eastern Cape Financial crises -- South Africa -- Eastern Cape Debts, Public -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10353/2038 , vital:27600
- Description: The view of on-site school support for curriculum delivery is shared by most countries and its effects have been felt by schools. There is fear that the Austerity Measures will negate the outcomes of the action taken by the teams that visit the schools. The reviewed literature is more biased towards the Austerity Measures in the whole government sector rather than in a department or an institution like the Education District in Idutywa. Even though the effects of Austerity Measures have been researched all over the world based on a specific country, there is deficiency of such literature done in the institution like the department of Education Districts. The researcher intends contributing to the filling of this gap by this study. The researcher therefore carried out a focused study of the effect of Department’s Austerity Measures on on-site school curriculum support in Idutywa Education District. It is also imperative to know how the teachers are affected by these departmental Austerity Measures, hence the interviews were carried out with the school personnel in addition to the District Professional staff. The District is characterised by poor performance in both Annual National Assessment (ANA) and the final National Senior Certificate results. The findings revealed that the implementation of AM have contributed to, amongst other things, the following issues: The inadequate on-site school support for curriculum delivery; The shortage of resources that includes teachers and vehicles; and ultimately the learner underperformance The researcher expect that the recommendation made will be embraced and be factored through, during the planning process of the Eastern Cape Department of Basic Education in order to improve learner performance.
- Full Text:
- Date Issued: 2016
- Authors: Lombo, Nomachule
- Date: 2016
- Subjects: Government spending policy -- South Africa -- Eastern Cape Financial crises -- South Africa -- Eastern Cape Debts, Public -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10353/2038 , vital:27600
- Description: The view of on-site school support for curriculum delivery is shared by most countries and its effects have been felt by schools. There is fear that the Austerity Measures will negate the outcomes of the action taken by the teams that visit the schools. The reviewed literature is more biased towards the Austerity Measures in the whole government sector rather than in a department or an institution like the Education District in Idutywa. Even though the effects of Austerity Measures have been researched all over the world based on a specific country, there is deficiency of such literature done in the institution like the department of Education Districts. The researcher intends contributing to the filling of this gap by this study. The researcher therefore carried out a focused study of the effect of Department’s Austerity Measures on on-site school curriculum support in Idutywa Education District. It is also imperative to know how the teachers are affected by these departmental Austerity Measures, hence the interviews were carried out with the school personnel in addition to the District Professional staff. The District is characterised by poor performance in both Annual National Assessment (ANA) and the final National Senior Certificate results. The findings revealed that the implementation of AM have contributed to, amongst other things, the following issues: The inadequate on-site school support for curriculum delivery; The shortage of resources that includes teachers and vehicles; and ultimately the learner underperformance The researcher expect that the recommendation made will be embraced and be factored through, during the planning process of the Eastern Cape Department of Basic Education in order to improve learner performance.
- Full Text:
- Date Issued: 2016
Assessment of rangeland condition and evaluation of farmers' perception in Njwaxa and Mbizana communal rangelands of the Eastern Cape, South Africa
- Authors: Ngcofe, Odwa Armstrong
- Date: 2016
- Subjects: Rangelands -- South Africa -- Eastern Cape Land tenure -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/13259 , vital:39627
- Description: This study was conducted to determine of rangeland condition in terms of botanical composition, soil nutrient composition, and also to evaluate farmers’ perception on rangeland condition and degradation in two communal villages Njwaxa and Mbizana in the Eastern Cape Province of South Africa in 2014. A Structured questionnaire consisting of close and open-ended questions was used to investigate farmers’ perceptions on range condition, land degradation, and their causes. Fifty households with livestock were randomly selected in two communal areas of Middledrift (i.e. Njwaxa and Mbizana). Respondents of 21 years and above were selected. For assessment of range condition three homogeneous vegetation units (HVU) were demarcated in all study sites, namely slope; valley and toplands were demarcated and sub-divided into four 100m x 50m replicates. In each replicate, four 100m transects were laid parallel to each other 25m apart 1m2 quadrant was systematically located four times along each transect for herbaceous vegetation and soil sampling. The step point method was employed to determine herbaceous species composition and basal cover. A 400m2 belt transect was used to determine woody vegetation composition, density and tree equivalents in each HVU. The germination method was employed for soil seed bank composition and plant density. The soil micro and macro nutrients (N, P, K OC, Ca, Mg, Zn, Cu and Mn) and pH were also analyzed The majority of the respondents were between the ages of 51-60 years. Most of the farmers had more cattle (38percent) than goats (32percent) and sheep (30percent). Livestock numbers were perceived to have declined over the previous 20 years by the majority of respondents (96percent). The respondents indicated that their rangelands were mainly used for browsing (1.37), fire wood (2.01), building and fencing (2.63) and medical purposes (3.66). All the respondents indicated that their rangelands were highly degraded. Sheet erosion (50percent) was perceived to be the major type of soil erosion indicator mentioned by the farmers, followed by gully erosion (29percent) and rill erosion (21percent). The rangeland was dominated by Increaser II (pioneer) (63.2 percent) grass species followed by Decreaser (mesophytes) species (26.3percent). Digitaria eriantha in the valley (53.5 percent) was significantly higher (p<0.05) than both in the top (10.5percent) and sloppy areas (33.5percent) at Njwaxa communal rangeland, the abundance of D. eriantha was significantly higher (p<0.05) than top and valley in Mbizana communal rangeland. The results showed that soil seed banks were dominated by perennials, followed by annuals and biennial species being least abundant. The dominant forb Jamebrittenia c.f. albanesis was significantly higher (p<0.05) in both slopes (6.65percent) and toplands (7.34percent) than in the valley (3.15). The second most dominant species Puesdognaphalium undulata was significantly higher (p<0.05) in topland areas (6percent) than in valley bottom (3.8percent) and sloppy areas (4.3percent). The results of soil nutrients revealed no significant differences (p>0.05) in the concentration of OC, Ca, and Mg between the communities and seasons. Phosphorus (P) was significantly higher (p<0.05) in winter (concentrations) in most of the sites, except the top sites of Mbizana where summer concentration of P was significantly higher than winter. Potassium (K) was significantly higher (p<0.05) in summer than in winter in most of the HVUs, except the topland areas of Njwaxa and Mbizana sloppy areas where there were no significant differences (p>0.05) in the concentration of K. Soil pH did not display any significant differences (p>0.05) between communities and sites. The mean soil pH recorded in this study was 5.4 implying the soils of Njwaxa and Mbizana communities were acidic. It is evident that communal farmers are well aware of their rangeland condition, changes occurring in their rangelands, their causes and are capable of suggesting some solutions in controlling the declining condition of the rangelands. This study has shown the importance of engaging farmers’ inputs in range assessment since those are people bearing the consequences of rangeland degradation.
- Full Text:
- Date Issued: 2016
- Authors: Ngcofe, Odwa Armstrong
- Date: 2016
- Subjects: Rangelands -- South Africa -- Eastern Cape Land tenure -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/13259 , vital:39627
- Description: This study was conducted to determine of rangeland condition in terms of botanical composition, soil nutrient composition, and also to evaluate farmers’ perception on rangeland condition and degradation in two communal villages Njwaxa and Mbizana in the Eastern Cape Province of South Africa in 2014. A Structured questionnaire consisting of close and open-ended questions was used to investigate farmers’ perceptions on range condition, land degradation, and their causes. Fifty households with livestock were randomly selected in two communal areas of Middledrift (i.e. Njwaxa and Mbizana). Respondents of 21 years and above were selected. For assessment of range condition three homogeneous vegetation units (HVU) were demarcated in all study sites, namely slope; valley and toplands were demarcated and sub-divided into four 100m x 50m replicates. In each replicate, four 100m transects were laid parallel to each other 25m apart 1m2 quadrant was systematically located four times along each transect for herbaceous vegetation and soil sampling. The step point method was employed to determine herbaceous species composition and basal cover. A 400m2 belt transect was used to determine woody vegetation composition, density and tree equivalents in each HVU. The germination method was employed for soil seed bank composition and plant density. The soil micro and macro nutrients (N, P, K OC, Ca, Mg, Zn, Cu and Mn) and pH were also analyzed The majority of the respondents were between the ages of 51-60 years. Most of the farmers had more cattle (38percent) than goats (32percent) and sheep (30percent). Livestock numbers were perceived to have declined over the previous 20 years by the majority of respondents (96percent). The respondents indicated that their rangelands were mainly used for browsing (1.37), fire wood (2.01), building and fencing (2.63) and medical purposes (3.66). All the respondents indicated that their rangelands were highly degraded. Sheet erosion (50percent) was perceived to be the major type of soil erosion indicator mentioned by the farmers, followed by gully erosion (29percent) and rill erosion (21percent). The rangeland was dominated by Increaser II (pioneer) (63.2 percent) grass species followed by Decreaser (mesophytes) species (26.3percent). Digitaria eriantha in the valley (53.5 percent) was significantly higher (p<0.05) than both in the top (10.5percent) and sloppy areas (33.5percent) at Njwaxa communal rangeland, the abundance of D. eriantha was significantly higher (p<0.05) than top and valley in Mbizana communal rangeland. The results showed that soil seed banks were dominated by perennials, followed by annuals and biennial species being least abundant. The dominant forb Jamebrittenia c.f. albanesis was significantly higher (p<0.05) in both slopes (6.65percent) and toplands (7.34percent) than in the valley (3.15). The second most dominant species Puesdognaphalium undulata was significantly higher (p<0.05) in topland areas (6percent) than in valley bottom (3.8percent) and sloppy areas (4.3percent). The results of soil nutrients revealed no significant differences (p>0.05) in the concentration of OC, Ca, and Mg between the communities and seasons. Phosphorus (P) was significantly higher (p<0.05) in winter (concentrations) in most of the sites, except the top sites of Mbizana where summer concentration of P was significantly higher than winter. Potassium (K) was significantly higher (p<0.05) in summer than in winter in most of the HVUs, except the topland areas of Njwaxa and Mbizana sloppy areas where there were no significant differences (p>0.05) in the concentration of K. Soil pH did not display any significant differences (p>0.05) between communities and sites. The mean soil pH recorded in this study was 5.4 implying the soils of Njwaxa and Mbizana communities were acidic. It is evident that communal farmers are well aware of their rangeland condition, changes occurring in their rangelands, their causes and are capable of suggesting some solutions in controlling the declining condition of the rangelands. This study has shown the importance of engaging farmers’ inputs in range assessment since those are people bearing the consequences of rangeland degradation.
- Full Text:
- Date Issued: 2016
Assessment of the flocculating efficiency of bioflocculant produced by bacillus sp. Aemreg4 isolated from Tyhume river, Eastern Cape, South Africa
- Authors: Ntsangani, Nozipho
- Date: 2016
- Subjects: Flocculants
- Language: English
- Type: Thesis , Masters , MSc (Biochemistry)
- Identifier: vital:11357 , http://hdl.handle.net/10353/d1021324
- Description: Bioflocculants are flocculating substances produced by microorganisms during growth and have recently received considerable attention from researchers; due to their biodegradability, non-toxicity and lack of secondary pollution from degradation intermediates. This study evaluated the efficiency of bioflocculant produced by Bacillus sp. AEMREG4 isolated from Tyhume River. The bacterial identification was through 16S rDNA sequencing; nucleotide sequences were deposited in GenBank as Bacillus sp. AEMREG4 with an Accession number KP406729. The optimum culture conditions for bioflocculant production were an inoculum size of 4% (v/v) and starch as well as yeast extract as sole carbon and nitrogen sources respectively. The addition of CaCl2 enhanced the flocculating activity, at a wide range of pH 4-10 and the highest flocculating activity was reached at an initial pH 8 (80%). A bioflocculant yield of 0.78 g was recovered from 1 L of culture broth. The optimum flocculating activity of 78% was reached at the lowest bioflocculant dosage of 0.1 mg/ml and the presence of divalent cations (Ca2+, Mn2+ and Mg2+) as well as a trivalent cation (Al3+) enhanced flocculating activity. The purified bioflocculant retained more than 70% flocculating activity when subjected to heating at 100 °C for 1 h and maximum flocculating activity of 83% was achieved at both acidic and basic pH values of 3 and 10 respectively. Chemical analysis showed that the bioflocculant is predominantly polysaccharide. The Fourier transform infrared (FTIR) spectrum revealed the presence of carboxyl, hydroxyl and methoxyl groups as the functional moieties and the scanning electron microscopy (SEM) imaging of the purified bioflocculant showed its morphological structure as rod-shaped which contributes to its high flocculating efficiency. The high flocculation activity displayed by this bioflocculant indicates its potential suitability for industrial application.Keywords: Bioflocculant, Bacillus sp. AEMREG4, flocculating activity.
- Full Text:
- Date Issued: 2016
- Authors: Ntsangani, Nozipho
- Date: 2016
- Subjects: Flocculants
- Language: English
- Type: Thesis , Masters , MSc (Biochemistry)
- Identifier: vital:11357 , http://hdl.handle.net/10353/d1021324
- Description: Bioflocculants are flocculating substances produced by microorganisms during growth and have recently received considerable attention from researchers; due to their biodegradability, non-toxicity and lack of secondary pollution from degradation intermediates. This study evaluated the efficiency of bioflocculant produced by Bacillus sp. AEMREG4 isolated from Tyhume River. The bacterial identification was through 16S rDNA sequencing; nucleotide sequences were deposited in GenBank as Bacillus sp. AEMREG4 with an Accession number KP406729. The optimum culture conditions for bioflocculant production were an inoculum size of 4% (v/v) and starch as well as yeast extract as sole carbon and nitrogen sources respectively. The addition of CaCl2 enhanced the flocculating activity, at a wide range of pH 4-10 and the highest flocculating activity was reached at an initial pH 8 (80%). A bioflocculant yield of 0.78 g was recovered from 1 L of culture broth. The optimum flocculating activity of 78% was reached at the lowest bioflocculant dosage of 0.1 mg/ml and the presence of divalent cations (Ca2+, Mn2+ and Mg2+) as well as a trivalent cation (Al3+) enhanced flocculating activity. The purified bioflocculant retained more than 70% flocculating activity when subjected to heating at 100 °C for 1 h and maximum flocculating activity of 83% was achieved at both acidic and basic pH values of 3 and 10 respectively. Chemical analysis showed that the bioflocculant is predominantly polysaccharide. The Fourier transform infrared (FTIR) spectrum revealed the presence of carboxyl, hydroxyl and methoxyl groups as the functional moieties and the scanning electron microscopy (SEM) imaging of the purified bioflocculant showed its morphological structure as rod-shaped which contributes to its high flocculating efficiency. The high flocculation activity displayed by this bioflocculant indicates its potential suitability for industrial application.Keywords: Bioflocculant, Bacillus sp. AEMREG4, flocculating activity.
- Full Text:
- Date Issued: 2016
Auditory processing problems within the inclusive foundation phase classroom: an exploration of teachers' experiences
- Authors: Deysel, Sanet
- Date: 2016
- Subjects: Inclusive education , Early childhood special education , Early childhood teachers
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10948/6834 , vital:21152
- Description: The Salamanca Statement (UNESCO, 1994) called upon all governments to implement inclusive education, ensuring that all learners with barriers to learning are included in the educational system. South Africa as a cosignatory to this global call responded with the implementation of the South African Education White Paper 6 (Department of Education, 2001) where the principles and foundations towards inclusive education were stipulated. It was expected of teachers to be able to accommodate learners with barriers to learning in their classrooms (Dednam, 2009, p. 371), although Ntombela and Green (2013, p. 2) state that teachers are not equipped to work with learners with specific disabilities. Learners present with various barriers to learning and these barriers pose problems and challenges in the classroom. One of these problems in the classroom is learners presenting with Auditory Processing Disorder. This qualitative study employed phenomenology as the research design. Through the use of memory work, drawings and focus group discussions as data production tools, the five Foundation Phase teachers’ experiences regarding learners presenting with Auditory Processing Disorder in the inclusive classroom, were explored. Bronfenbrenner’s ecological model was used to make meaning of the findings of the study. The findings of the study indicate that teachers are torn between the expectations of global and national policies regarding inclusive education and the management and support of learners presenting with Auditory Processing Disorder in their classrooms. Various challenges and problems arise with the inclusion of learners presenting with Auditory Processing Disorder in the classroom. The findings of the study were used to formulate guidelines to support Foundation Phase teachers working with learners presenting with auditory processing problems as well as policy suggestions for the Department of Basic Education. The Department of Basic Education should revise the implementation of CAPS to include the necessary adaptations for learners presenting with Auditory Processing Disorder; and also provide teacher assistants in Foundation Phase classrooms to enable the full inclusion of all learners.
- Full Text:
- Date Issued: 2016
- Authors: Deysel, Sanet
- Date: 2016
- Subjects: Inclusive education , Early childhood special education , Early childhood teachers
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10948/6834 , vital:21152
- Description: The Salamanca Statement (UNESCO, 1994) called upon all governments to implement inclusive education, ensuring that all learners with barriers to learning are included in the educational system. South Africa as a cosignatory to this global call responded with the implementation of the South African Education White Paper 6 (Department of Education, 2001) where the principles and foundations towards inclusive education were stipulated. It was expected of teachers to be able to accommodate learners with barriers to learning in their classrooms (Dednam, 2009, p. 371), although Ntombela and Green (2013, p. 2) state that teachers are not equipped to work with learners with specific disabilities. Learners present with various barriers to learning and these barriers pose problems and challenges in the classroom. One of these problems in the classroom is learners presenting with Auditory Processing Disorder. This qualitative study employed phenomenology as the research design. Through the use of memory work, drawings and focus group discussions as data production tools, the five Foundation Phase teachers’ experiences regarding learners presenting with Auditory Processing Disorder in the inclusive classroom, were explored. Bronfenbrenner’s ecological model was used to make meaning of the findings of the study. The findings of the study indicate that teachers are torn between the expectations of global and national policies regarding inclusive education and the management and support of learners presenting with Auditory Processing Disorder in their classrooms. Various challenges and problems arise with the inclusion of learners presenting with Auditory Processing Disorder in the classroom. The findings of the study were used to formulate guidelines to support Foundation Phase teachers working with learners presenting with auditory processing problems as well as policy suggestions for the Department of Basic Education. The Department of Basic Education should revise the implementation of CAPS to include the necessary adaptations for learners presenting with Auditory Processing Disorder; and also provide teacher assistants in Foundation Phase classrooms to enable the full inclusion of all learners.
- Full Text:
- Date Issued: 2016
A memory work project for Marikana
- Authors: Snyman, Emile Alexander
- Date: 2016
- Subjects: Monuments -- South Africa -- Rustenburg Memorials -- South Africa -- Rustenburg
- Language: English
- Type: Thesis , Masters , MArch
- Identifier: http://hdl.handle.net/10948/38885 , vital:35010
- Description: This treatise suggests one possible strategy for engaging the memory of the Marikana Massacre through different representational modes. Part 1 of this treatise takes the form of the research conducted, which was explorative in nature and focused on coming to an understanding of collective memory, the role of monuments and memorials within this process, the contemporary South African memorial landscape and the events that transpired at Marikana between 9 and 16 August 2012 and what their historical significance may be. As it was found that strikes featuring on the platinum belt were not only related to wage issues, Marikana as a settlement within the context of South Africa’s platinum belt was further investigated in order to highlight what the multiple challenges and issues are with which communities are faced on a daily basis. Part 2 sets out the strategy of a memory work project for Marikana which involves three components, namely a web- and phone-based application called ‘Remembering Marikana’ which provides a discursive arena for the politics of memory, a memorial for the victims of the Marikana Massacre at Thaba (Marikana Mountain) which facilitates annual commemoration of the massacre, and the design of a Mgcineni Noki Memorial Sports Centre, a community institution which embodies the values of the strike leaders and makes a positive contribution to the impoverished urban economy of the Wonderkop neighbourhood in Marikana.
- Full Text:
- Date Issued: 2016
- Authors: Snyman, Emile Alexander
- Date: 2016
- Subjects: Monuments -- South Africa -- Rustenburg Memorials -- South Africa -- Rustenburg
- Language: English
- Type: Thesis , Masters , MArch
- Identifier: http://hdl.handle.net/10948/38885 , vital:35010
- Description: This treatise suggests one possible strategy for engaging the memory of the Marikana Massacre through different representational modes. Part 1 of this treatise takes the form of the research conducted, which was explorative in nature and focused on coming to an understanding of collective memory, the role of monuments and memorials within this process, the contemporary South African memorial landscape and the events that transpired at Marikana between 9 and 16 August 2012 and what their historical significance may be. As it was found that strikes featuring on the platinum belt were not only related to wage issues, Marikana as a settlement within the context of South Africa’s platinum belt was further investigated in order to highlight what the multiple challenges and issues are with which communities are faced on a daily basis. Part 2 sets out the strategy of a memory work project for Marikana which involves three components, namely a web- and phone-based application called ‘Remembering Marikana’ which provides a discursive arena for the politics of memory, a memorial for the victims of the Marikana Massacre at Thaba (Marikana Mountain) which facilitates annual commemoration of the massacre, and the design of a Mgcineni Noki Memorial Sports Centre, a community institution which embodies the values of the strike leaders and makes a positive contribution to the impoverished urban economy of the Wonderkop neighbourhood in Marikana.
- Full Text:
- Date Issued: 2016
Bayesian accelerated life tests: exponential and Weibull models
- Authors: Izally, Sharkay Ruwade
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3003 , vital:20351
- Description: Reliability life testing is used for life data analysis in which samples are tested under normal conditions to obtain failure time data for reliability assessment. It can be costly and time consuming to obtain failure time data under normal operating conditions if the mean time to failure of a product is long. An alternative is to use failure time data from an accelerated life test (ALT) to extrapolate the reliability under normal conditions. In accelerated life testing, the units are placed under a higher than normal stress condition such as voltage, current, pressure, temperature, to make the items fail in a shorter period of time. The failure information is then transformed through an accelerated model commonly known as the time transformation function, to predict the reliability under normal operating conditions. The power law will be used as the time transformation function in this thesis. We will first consider a Bayesian inference model under the assumption that the underlying life distribution in the accelerated life test is exponentially distributed. The maximal data information (MDI) prior, the Ghosh Mergel and Liu (GML) prior and the Jeffreys prior will be derived for the exponential distribution. The propriety of the posterior distributions will be investigated. Results will be compared when using these non-informative priors in a simulation study by looking at the posterior variances. The Weibull distribution as the underlying life distribution in the accelerated life test will also be investigated. The maximal data information prior will be derived for the Weibull distribution using the power law. The uniform prior and a mixture of Gamma and uniform priors will be considered. The propriety of these posteriors will also be investigated. The predictive reliability at the use-stress will be computed for these models. The deviance information criterion will be used to compare these priors. As a result of using a time transformation function, Bayesian inference becomes analytically intractable and Markov Chain Monte Carlo (MCMC) methods will be used to alleviate this problem. The Metropolis-Hastings algorithm will be used to sample from the posteriors for the exponential model in the accelerated life test. The adaptive rejection sampling method will be used to sample from the posterior distributions when the Weibull model is considered.
- Full Text:
- Date Issued: 2016
- Authors: Izally, Sharkay Ruwade
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3003 , vital:20351
- Description: Reliability life testing is used for life data analysis in which samples are tested under normal conditions to obtain failure time data for reliability assessment. It can be costly and time consuming to obtain failure time data under normal operating conditions if the mean time to failure of a product is long. An alternative is to use failure time data from an accelerated life test (ALT) to extrapolate the reliability under normal conditions. In accelerated life testing, the units are placed under a higher than normal stress condition such as voltage, current, pressure, temperature, to make the items fail in a shorter period of time. The failure information is then transformed through an accelerated model commonly known as the time transformation function, to predict the reliability under normal operating conditions. The power law will be used as the time transformation function in this thesis. We will first consider a Bayesian inference model under the assumption that the underlying life distribution in the accelerated life test is exponentially distributed. The maximal data information (MDI) prior, the Ghosh Mergel and Liu (GML) prior and the Jeffreys prior will be derived for the exponential distribution. The propriety of the posterior distributions will be investigated. Results will be compared when using these non-informative priors in a simulation study by looking at the posterior variances. The Weibull distribution as the underlying life distribution in the accelerated life test will also be investigated. The maximal data information prior will be derived for the Weibull distribution using the power law. The uniform prior and a mixture of Gamma and uniform priors will be considered. The propriety of these posteriors will also be investigated. The predictive reliability at the use-stress will be computed for these models. The deviance information criterion will be used to compare these priors. As a result of using a time transformation function, Bayesian inference becomes analytically intractable and Markov Chain Monte Carlo (MCMC) methods will be used to alleviate this problem. The Metropolis-Hastings algorithm will be used to sample from the posteriors for the exponential model in the accelerated life test. The adaptive rejection sampling method will be used to sample from the posterior distributions when the Weibull model is considered.
- Full Text:
- Date Issued: 2016
Being civil
- Matshoba, Zongezile Theophilus
- Authors: Matshoba, Zongezile Theophilus
- Date: 2016
- Language: English , Xhosa
- Type: Thesis , Masters , MA
- Identifier: vital:6017 , http://hdl.handle.net/10962/d1021237
- Description: My collection of short stories delves into government and governance, democracy, citizenship, civil servants, poverty, corruption and nepotism. My stories draw on the traditions of gritty urban crime fiction uncovering crimes of violence, service delivery, vandalism and corruption. They explore themes of mental cruelty and greed, self- preservation and community in rural areas, farms, townships and cities characterized by wrenching contradictions and inequalities. , This epic dramatic poetic verse delves into government and school governance, labour unions, liberation struggle, parenting and a wide range of school perceptions. It interrogates the roles of parents, teachers, students, department of education officials and that of other stakeholders that make use of schools. Influenced by William Wellington Gqoba’s ‘A great debate on education: a Parable’ wayback, it continues the education debate in the current post-democratic South Africa characterized by wrenching contradictions and inequalities. , This thesis is presented in two parts: English and isiXhosa.
- Full Text:
- Date Issued: 2016
- Authors: Matshoba, Zongezile Theophilus
- Date: 2016
- Language: English , Xhosa
- Type: Thesis , Masters , MA
- Identifier: vital:6017 , http://hdl.handle.net/10962/d1021237
- Description: My collection of short stories delves into government and governance, democracy, citizenship, civil servants, poverty, corruption and nepotism. My stories draw on the traditions of gritty urban crime fiction uncovering crimes of violence, service delivery, vandalism and corruption. They explore themes of mental cruelty and greed, self- preservation and community in rural areas, farms, townships and cities characterized by wrenching contradictions and inequalities. , This epic dramatic poetic verse delves into government and school governance, labour unions, liberation struggle, parenting and a wide range of school perceptions. It interrogates the roles of parents, teachers, students, department of education officials and that of other stakeholders that make use of schools. Influenced by William Wellington Gqoba’s ‘A great debate on education: a Parable’ wayback, it continues the education debate in the current post-democratic South Africa characterized by wrenching contradictions and inequalities. , This thesis is presented in two parts: English and isiXhosa.
- Full Text:
- Date Issued: 2016
Being for the Other: Surveillance and Depictions of Race, Gender, and Animals in Contemporary South African Fiction
- Authors: Laue, Kharys Ateh
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/3848 , vital:20549
- Description: This thesis examines the depiction, in contemporary South African fiction, of irresponsibility and responsibility in relation to the raced, gendered, and animal Other. Through a close analysis of Jeremy Bentham’s Panopticon prison and Michel Foucault’s study of this design, I establish the notion of disciplinary surveillance or panopticism. This I take to be a mode of power that seeks, by means of an invisible gaze, to render its subjects docile. In my readings of J. M. Coetzee’s Waiting for the Barbarians, Zoë Wicomb’s Playing in the Light, Justin Cartwright’s White Lightning, and selected short stories from Wicomb’s You Can’t Get Lost in Cape Town and The One That Got Away, I demonstrate that oppressive authoritarian regimes are rooted in Benthamic principles of hyper-visibility and concealment. Disciplinary power, I contend, is effective precisely because it places an individual in a constant state of Being-for-Others, a term coined by Jean-Paul Sartre to describe the experience of objectification through another’s look. Judith Butler’s concept of gender performativity and W. E. B. Du Bois’s notion of black double consciousness frame my examination of, respectively, gender and racial oppression, while my discussion of animals appeals to Jacques Derrida’s work on the non-human. I show how surveillance, in each of the selected texts, functions through a racist and/or sexist and/or speciesist gaze that facilitates violent, irresponsible relationships with the human and non-human Other. The texts under discussion, however, also depict ways in which the Other actively resists and subverts regimes of oppression, often by means of a counter gaze that compels the protagonist, or the reader, to take up responsibility for Others. Ultimately, my study concludes that the fictional works of Coetzee, Wicomb, and Cartwright offer an ethics of empathetic responsibility, which I term Being for the Other, in opposition to mechanisms of disciplinary surveillance that seek to oppress, conceal, and dominate.
- Full Text:
- Date Issued: 2016
- Authors: Laue, Kharys Ateh
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/3848 , vital:20549
- Description: This thesis examines the depiction, in contemporary South African fiction, of irresponsibility and responsibility in relation to the raced, gendered, and animal Other. Through a close analysis of Jeremy Bentham’s Panopticon prison and Michel Foucault’s study of this design, I establish the notion of disciplinary surveillance or panopticism. This I take to be a mode of power that seeks, by means of an invisible gaze, to render its subjects docile. In my readings of J. M. Coetzee’s Waiting for the Barbarians, Zoë Wicomb’s Playing in the Light, Justin Cartwright’s White Lightning, and selected short stories from Wicomb’s You Can’t Get Lost in Cape Town and The One That Got Away, I demonstrate that oppressive authoritarian regimes are rooted in Benthamic principles of hyper-visibility and concealment. Disciplinary power, I contend, is effective precisely because it places an individual in a constant state of Being-for-Others, a term coined by Jean-Paul Sartre to describe the experience of objectification through another’s look. Judith Butler’s concept of gender performativity and W. E. B. Du Bois’s notion of black double consciousness frame my examination of, respectively, gender and racial oppression, while my discussion of animals appeals to Jacques Derrida’s work on the non-human. I show how surveillance, in each of the selected texts, functions through a racist and/or sexist and/or speciesist gaze that facilitates violent, irresponsible relationships with the human and non-human Other. The texts under discussion, however, also depict ways in which the Other actively resists and subverts regimes of oppression, often by means of a counter gaze that compels the protagonist, or the reader, to take up responsibility for Others. Ultimately, my study concludes that the fictional works of Coetzee, Wicomb, and Cartwright offer an ethics of empathetic responsibility, which I term Being for the Other, in opposition to mechanisms of disciplinary surveillance that seek to oppress, conceal, and dominate.
- Full Text:
- Date Issued: 2016
Beta decay of 100/400 Zr produced in neutron-induced fission of natural uranium
- Authors: Kamoto, Thokozani
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3024 , vital:20353
- Description: Fission fragments, produced by neutron bombardment of natural uranium at the Physics Department, Jyväskylä, Finland, are studied in this work. The data had been sorted into 25 Y — y coincidence matrices which were then analysed. In this work we aimed to identify the fission products using Y-Y coincidence analysis and then study the beta-decay of some of the fission products. Sixteen fission products ranging from A = 94 to A = 136 were identified. Out of these fission products beta decay of the A = 100 (100/40 Zr – 100/41 Nb – 100/42 Mo) chain was studied in greater detail. We have also studied the variation of the relative intensities as a function of time of the 159-, 528-, 600-, 768-, 928- and 1502-keV Y-rav lines in 100/42 Mo and the profiles of the relative intensities have been modelled with the variation of the activity of 100/41 Nb against time. Configuration assignments of 100 Zr and 100/42 Mo are discussed.
- Full Text:
- Date Issued: 2016
- Authors: Kamoto, Thokozani
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3024 , vital:20353
- Description: Fission fragments, produced by neutron bombardment of natural uranium at the Physics Department, Jyväskylä, Finland, are studied in this work. The data had been sorted into 25 Y — y coincidence matrices which were then analysed. In this work we aimed to identify the fission products using Y-Y coincidence analysis and then study the beta-decay of some of the fission products. Sixteen fission products ranging from A = 94 to A = 136 were identified. Out of these fission products beta decay of the A = 100 (100/40 Zr – 100/41 Nb – 100/42 Mo) chain was studied in greater detail. We have also studied the variation of the relative intensities as a function of time of the 159-, 528-, 600-, 768-, 928- and 1502-keV Y-rav lines in 100/42 Mo and the profiles of the relative intensities have been modelled with the variation of the activity of 100/41 Nb against time. Configuration assignments of 100 Zr and 100/42 Mo are discussed.
- Full Text:
- Date Issued: 2016
Between sentences
- Authors: Thesen, Jo-Ann
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6007 , http://hdl.handle.net/10962/1021215
- Description: My stories explore different forms, including flash fiction. Some use the fairy tale form to combine fiction and non-fiction in order to reach the essence of the story. In this I am influenced by Kate Bernheimer, who speaks of the “flatness, abstraction, intuitive logic and normalized magic” of traditional fairy tales. A number of stories are set in the places I worked as a newspaper reporter. Here I use my old press reports as starting points for the real or imagined story behind the news – often involving miscommunication, dominance, exploitation, the tension between isolation and belonging, and the nuances of family relationships.
- Full Text:
- Date Issued: 2016
- Authors: Thesen, Jo-Ann
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6007 , http://hdl.handle.net/10962/1021215
- Description: My stories explore different forms, including flash fiction. Some use the fairy tale form to combine fiction and non-fiction in order to reach the essence of the story. In this I am influenced by Kate Bernheimer, who speaks of the “flatness, abstraction, intuitive logic and normalized magic” of traditional fairy tales. A number of stories are set in the places I worked as a newspaper reporter. Here I use my old press reports as starting points for the real or imagined story behind the news – often involving miscommunication, dominance, exploitation, the tension between isolation and belonging, and the nuances of family relationships.
- Full Text:
- Date Issued: 2016
Between"culture" and "Cruelty"narratives of widows on land dispossession in Gqadu administrative area, Mbashe Municipality , Eastern Cape
- Authors: Baleka, Zola
- Date: 2016
- Subjects: Widows -- South Africa -- Eastern Cape Land tenure -- South Africa -- Eastern Cape Right of property -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , Degree
- Identifier: http://hdl.handle.net/10353/5083 , vital:29057
- Description: This study highlights the cultural underpinnings of specific indulgences pertaining to land ownership with reference to widowhood in the rural Eastern Cape. The study does this by examining the narratives of widows about their experiences of land dispossession in Nqadu Administrative Area. Nqadu Administrative Area is a rural community in Mbhashe Local Municipality in the Eastern Cape Province of South Africa. The study focuses on widows’ experiences relating to the cultural practices of the community regarding land ownership. A qualitative, case study design was adopted. To uncover the widows’ narratives, the study utilised in-depth interviews and focus group discussions, with a sample of 50 widows. Further empirical data were obtained from four members of the municipal Land Committees, two Ward Councillors, four Traditional Leaders and two officials from the Eastern Cape Department of Rural Development and Agrarian Reform, using in-depth and key informant interviews. The study found that widows were vulnerable to land ownership violations and dispossessions, and that these violations were perpetrated by community members, particularly relatives of deceased husbands. Low levels of education and fear of antagonising clan relatives played a vital role in preventing widows from fighting for their legal rights. The failure of widows to fight for their rights increased their chances of being heavily exposed to dispossession of their land rights. Cultural practices within the communities catalysed abusive practices against widows, particularly against those whose marriage was not ‘recognised’ due to various stated reasons. The study also revealed that widows who had married as second wives and those who did not bear children experienced egregious abuses. Another finding was that men from Nqadu Administrative Area were given priority during the allocation of land, based on cultural and traditional practices, and were given land of their own choice depending on availability. The study thus highlights the impact of cultural practices and the ways in which these became instruments of gender abuse, despite the existence of laws designed to prevent specific social cruelties.
- Full Text:
- Date Issued: 2016
- Authors: Baleka, Zola
- Date: 2016
- Subjects: Widows -- South Africa -- Eastern Cape Land tenure -- South Africa -- Eastern Cape Right of property -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , Degree
- Identifier: http://hdl.handle.net/10353/5083 , vital:29057
- Description: This study highlights the cultural underpinnings of specific indulgences pertaining to land ownership with reference to widowhood in the rural Eastern Cape. The study does this by examining the narratives of widows about their experiences of land dispossession in Nqadu Administrative Area. Nqadu Administrative Area is a rural community in Mbhashe Local Municipality in the Eastern Cape Province of South Africa. The study focuses on widows’ experiences relating to the cultural practices of the community regarding land ownership. A qualitative, case study design was adopted. To uncover the widows’ narratives, the study utilised in-depth interviews and focus group discussions, with a sample of 50 widows. Further empirical data were obtained from four members of the municipal Land Committees, two Ward Councillors, four Traditional Leaders and two officials from the Eastern Cape Department of Rural Development and Agrarian Reform, using in-depth and key informant interviews. The study found that widows were vulnerable to land ownership violations and dispossessions, and that these violations were perpetrated by community members, particularly relatives of deceased husbands. Low levels of education and fear of antagonising clan relatives played a vital role in preventing widows from fighting for their legal rights. The failure of widows to fight for their rights increased their chances of being heavily exposed to dispossession of their land rights. Cultural practices within the communities catalysed abusive practices against widows, particularly against those whose marriage was not ‘recognised’ due to various stated reasons. The study also revealed that widows who had married as second wives and those who did not bear children experienced egregious abuses. Another finding was that men from Nqadu Administrative Area were given priority during the allocation of land, based on cultural and traditional practices, and were given land of their own choice depending on availability. The study thus highlights the impact of cultural practices and the ways in which these became instruments of gender abuse, despite the existence of laws designed to prevent specific social cruelties.
- Full Text:
- Date Issued: 2016
Beyond commercial design: a critique of design and graphic design writings in Emigre and Dot Dot Dot magazines
- Authors: Muir, Margot
- Date: 2016
- Subjects: Graphic design (Typography) Graphic arts Magazine design
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/12436 , vital:27066
- Description: Graphic design faces the contradictions of commercial intent and social relevance. This study explores the contribution of criticism, in two independent, seminal graphic design magazines, towards shifting the dominant preferences of graphic design from a purely commercial pursuit to a human-centred practice. Emigre magazine (c.1984 - 2005) and Dot Dot Dot magazine (c.2000 - 2010) are recognised for their critical intent and within them are emerging critical issues that suggest a potential niche for graphic design beyond consumerism and commerce. In the discipline of graphic design, designers define what it is to be human (and thus equally the realities of dehumanisation) in very particular ways (Rose, 2001:135; Freire, 1993:43). Graphic design has a history of commercial practice. This commercial history continues to define its identity and reinforce a particular body of knowledge. Graphic design criticism, however, is an inventive voice that has the potential to contribute to change. Both Emigre and Dot Dot Dot were representative of a “constructive marginality” (Bennett, 1993:64), drawing from their own set of references and awareness of graphic design’s potential to inform their identities, instead of looking to established definitions of practice to do so. This analysis explores how they anticipated a modern conception of graphic design that has become part of a recently adopted (2015) and more widely embedded discourse. This discourse involves critical design that interrogates multiculturalism, interdisciplinarity, environmental sustainability, social and political agency, and speculative futures. Graphic design engages social institutions and practices that denote social constructions of difference and inequality, and is never neutral. Any work, any representation of ideology, is at once individual and discursive at the level of social, cultural and political formations. The critical issues evident in Emigre and Dot Dot Dot, with the exception of an absence of speculative futures, anticipate a more humanising perspective in graphic design. They invite critique and the potential for change that is relevant to the surrounding world, as a counter to commercial self-interest.
- Full Text:
- Date Issued: 2016
- Authors: Muir, Margot
- Date: 2016
- Subjects: Graphic design (Typography) Graphic arts Magazine design
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/12436 , vital:27066
- Description: Graphic design faces the contradictions of commercial intent and social relevance. This study explores the contribution of criticism, in two independent, seminal graphic design magazines, towards shifting the dominant preferences of graphic design from a purely commercial pursuit to a human-centred practice. Emigre magazine (c.1984 - 2005) and Dot Dot Dot magazine (c.2000 - 2010) are recognised for their critical intent and within them are emerging critical issues that suggest a potential niche for graphic design beyond consumerism and commerce. In the discipline of graphic design, designers define what it is to be human (and thus equally the realities of dehumanisation) in very particular ways (Rose, 2001:135; Freire, 1993:43). Graphic design has a history of commercial practice. This commercial history continues to define its identity and reinforce a particular body of knowledge. Graphic design criticism, however, is an inventive voice that has the potential to contribute to change. Both Emigre and Dot Dot Dot were representative of a “constructive marginality” (Bennett, 1993:64), drawing from their own set of references and awareness of graphic design’s potential to inform their identities, instead of looking to established definitions of practice to do so. This analysis explores how they anticipated a modern conception of graphic design that has become part of a recently adopted (2015) and more widely embedded discourse. This discourse involves critical design that interrogates multiculturalism, interdisciplinarity, environmental sustainability, social and political agency, and speculative futures. Graphic design engages social institutions and practices that denote social constructions of difference and inequality, and is never neutral. Any work, any representation of ideology, is at once individual and discursive at the level of social, cultural and political formations. The critical issues evident in Emigre and Dot Dot Dot, with the exception of an absence of speculative futures, anticipate a more humanising perspective in graphic design. They invite critique and the potential for change that is relevant to the surrounding world, as a counter to commercial self-interest.
- Full Text:
- Date Issued: 2016
Bio-prospecting a Soil Metagenomic Library for Carbohydrate Active Esterases
- Authors: Shezi, Ntombifuthi
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4172 , http://hdl.handle.net/10962/d1021266
- Description: Lignocellulosic biomass is a promising renewable resource on earth. Plant biomass contains fermentable sugars and other moieties that can be converted to biofuels or other chemicals. Enzymatic hydrolysis of these biopolymers is significant in the liberation of sugars for fermentation into desired products. Owing to its complex structure, synergistic action of enzymes is required for its degradation. Enzymes that are involved in biomass degradation include cellulases, hemicellulases and the accessory enzymes acetyl xylan esterases and ferulic acid esterases. Ferulic acid esterases (FAEs, EC 3.1.1.73), represent a subclass of carboxylester hydrolases (EC 3.1.1.-) that catalyse the release of hydroxycinnamic acids (such as ferulic acid, p-coumaric, ferulic, sinapic and caffeic acid) that are generally found esterified to polysaccharides, such as arabinoxylans. Hydroxycinnamic acids have widespread potential applications due to their antimicrobial, photoprotectant and antioxidant properties, as well as their use as flavour precursors. Therefore, this interesting group of FAEs has a potentially wide variety of applications in agriculture, food and pharmaceutical industries. In the search for novel biocatalysts, metagenomics is considered as an alternative approach to conventional microbe screening, therefore, searching for novel biocatalysts from a soil metagenome that harbours a unique diversity of biocatalyst is significant. The aim of this study was to extract DNA from soil associated with cattle manure and construct a soil metagenomic library using a fosmid based plasmid vector and subsequently functionally screen for ferulic acid esterases using ethyl ferulate as a model substrate. A total of 59 recombinant fosmids conferring ferulic acid esterase phenotypes were identified (Hit rate 1:3122) and the two fosmids that consistently showed high FAE activities were selected for further study. Following nucleotide sequencing and translational analysis, two fae encoding open reading frames (FAE9 and FAE27) of approximately 274 and 322 aa, respectively, were identified. The amino acid sequence of the two ORFs contained a classical conserved esterase/lipase G-x-S-x-G sequence motif. The two genes (fae9 and fae27) were successfully expressed in Escherichia coli BL21 (DE3) and the purified enzymes exhibited respective temperature optima of 50 °C and 40 °C, and respective pH optima of 6.0 and 7.0. Further biochemical characterisation showed that FAE9 and FAE27 have high substrate specificity, following the fact that EFA is the preferred substrate for FAE9 (kcat/Km value of 128 s−1.mM-1) and also the preferred substrate for FAE27 (kcat/Km value of 137 s−1.mM-1). This work proves that soil is a valuable environmental source for novel esterase screening through functional based metagenomic approach. Therefore, this method may be used to screen for other valuable enzymes from environmental sources using inexpensive natural sources to encourage the screening of specific enzymes. Biochemistry of the two isolated enzymes makes these enzymes to be useful in industrial applications due to broad substrate activity that could replace the specialised enzymes to complete plant biomass degradation.
- Full Text:
- Date Issued: 2016
- Authors: Shezi, Ntombifuthi
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4172 , http://hdl.handle.net/10962/d1021266
- Description: Lignocellulosic biomass is a promising renewable resource on earth. Plant biomass contains fermentable sugars and other moieties that can be converted to biofuels or other chemicals. Enzymatic hydrolysis of these biopolymers is significant in the liberation of sugars for fermentation into desired products. Owing to its complex structure, synergistic action of enzymes is required for its degradation. Enzymes that are involved in biomass degradation include cellulases, hemicellulases and the accessory enzymes acetyl xylan esterases and ferulic acid esterases. Ferulic acid esterases (FAEs, EC 3.1.1.73), represent a subclass of carboxylester hydrolases (EC 3.1.1.-) that catalyse the release of hydroxycinnamic acids (such as ferulic acid, p-coumaric, ferulic, sinapic and caffeic acid) that are generally found esterified to polysaccharides, such as arabinoxylans. Hydroxycinnamic acids have widespread potential applications due to their antimicrobial, photoprotectant and antioxidant properties, as well as their use as flavour precursors. Therefore, this interesting group of FAEs has a potentially wide variety of applications in agriculture, food and pharmaceutical industries. In the search for novel biocatalysts, metagenomics is considered as an alternative approach to conventional microbe screening, therefore, searching for novel biocatalysts from a soil metagenome that harbours a unique diversity of biocatalyst is significant. The aim of this study was to extract DNA from soil associated with cattle manure and construct a soil metagenomic library using a fosmid based plasmid vector and subsequently functionally screen for ferulic acid esterases using ethyl ferulate as a model substrate. A total of 59 recombinant fosmids conferring ferulic acid esterase phenotypes were identified (Hit rate 1:3122) and the two fosmids that consistently showed high FAE activities were selected for further study. Following nucleotide sequencing and translational analysis, two fae encoding open reading frames (FAE9 and FAE27) of approximately 274 and 322 aa, respectively, were identified. The amino acid sequence of the two ORFs contained a classical conserved esterase/lipase G-x-S-x-G sequence motif. The two genes (fae9 and fae27) were successfully expressed in Escherichia coli BL21 (DE3) and the purified enzymes exhibited respective temperature optima of 50 °C and 40 °C, and respective pH optima of 6.0 and 7.0. Further biochemical characterisation showed that FAE9 and FAE27 have high substrate specificity, following the fact that EFA is the preferred substrate for FAE9 (kcat/Km value of 128 s−1.mM-1) and also the preferred substrate for FAE27 (kcat/Km value of 137 s−1.mM-1). This work proves that soil is a valuable environmental source for novel esterase screening through functional based metagenomic approach. Therefore, this method may be used to screen for other valuable enzymes from environmental sources using inexpensive natural sources to encourage the screening of specific enzymes. Biochemistry of the two isolated enzymes makes these enzymes to be useful in industrial applications due to broad substrate activity that could replace the specialised enzymes to complete plant biomass degradation.
- Full Text:
- Date Issued: 2016