Investigation of Sexual Relationships among gay students at the University Of Fort Hare (Alice Campus), Eastern Cape.
- Authors: Mbanga , T.Q
- Date: 2019
- Subjects: Gay youth Sex (Psychology)
- Language: English
- Type: Thesis , Masters , MSoc. Sci (Psychology)
- Identifier: http://hdl.handle.net/10353/17619 , vital:41129
- Description: The main aim of the study was to investigate sexual relationships among gay students at the University of Fort Hare (Alice Campus), Eastern Cape. A qualitative research design was followed and semi-structured interview schedules were issued to twelve participants. The queer theory underpinned this study and it is based on the notion that individuals are able to determine their sexual identity and sexual orientation as they are. The participants, aged 18-25 years, were purposively selected to share their sexual behaviour as gays. The ethical principles such as informed consent, confidentiality, privacy and protection from harm, were adhered to, throughout the study. Prior identification of the themes, coding took place. The following themes were very important in the research and they helped to achieve the aim of the study and they are; challenges gay students experience in their relationships, closet partners, social networks sexual partners among gay students, condom use among gay students, sexual agreements among gay students, condom use, condom self-efficacy, honesty and communication, social support, self-esteem, liberal community and non-homophobic, traditional male circumcision, sexual agreements among gay students ,pornography viewing , sexual abuse among gay students, discrimination in residence, religiosity and perception towards marriage among gay students. Based on the findings, it is recommended that there should be communication among gay couples to avoid lot of conflicts. Also, there should be education about homosexuality in communities, schools and tertiary institutions as many people treat gays badly owing to lack of knowledge
- Full Text:
- Date Issued: 2019
- Authors: Mbanga , T.Q
- Date: 2019
- Subjects: Gay youth Sex (Psychology)
- Language: English
- Type: Thesis , Masters , MSoc. Sci (Psychology)
- Identifier: http://hdl.handle.net/10353/17619 , vital:41129
- Description: The main aim of the study was to investigate sexual relationships among gay students at the University of Fort Hare (Alice Campus), Eastern Cape. A qualitative research design was followed and semi-structured interview schedules were issued to twelve participants. The queer theory underpinned this study and it is based on the notion that individuals are able to determine their sexual identity and sexual orientation as they are. The participants, aged 18-25 years, were purposively selected to share their sexual behaviour as gays. The ethical principles such as informed consent, confidentiality, privacy and protection from harm, were adhered to, throughout the study. Prior identification of the themes, coding took place. The following themes were very important in the research and they helped to achieve the aim of the study and they are; challenges gay students experience in their relationships, closet partners, social networks sexual partners among gay students, condom use among gay students, sexual agreements among gay students, condom use, condom self-efficacy, honesty and communication, social support, self-esteem, liberal community and non-homophobic, traditional male circumcision, sexual agreements among gay students ,pornography viewing , sexual abuse among gay students, discrimination in residence, religiosity and perception towards marriage among gay students. Based on the findings, it is recommended that there should be communication among gay couples to avoid lot of conflicts. Also, there should be education about homosexuality in communities, schools and tertiary institutions as many people treat gays badly owing to lack of knowledge
- Full Text:
- Date Issued: 2019
Sequencing, assembly and annotation of the mitochondrial and plastid genomes of Gelidium pristoides (Turner) Kützing from Kenton-on-Sea, South Africa
- Authors: Mangali, Sandisiwe
- Date: 2019
- Subjects: Gelidium -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/19109 , vital:39883
- Description: The genome is the complete set of an organism's hereditary information that contains all the information necessary for the functioning of that organism. Complete nuclear, mitochondrial and plastid DNA constitute the three main types of genomes which play interconnected roles in an organism. Genome sequencing enables researchers to understand the regulation and expression of the various genes and the proteins they encode. It allows researchers to extract and analyse genes of interests for a variety of studies including molecular, biotechnological, bioinformatics and conservation and evolutionary studies. Genome sequencing of Rhodophyta has received little attention. To date, no published studies are focusing on both whole genome sequencing and sequencing of the organellar genomes of Rhodophyta species found in along the South African coastline. This study focused on genome sequencing, assembly and annotation mitochondrial and plastid genomes of Gelidium pristoides. Gelidium pristoides was collected from Kenton-on-Sea and was morphologically identified at Rhodes University. Its genomic DNA was extracted using the Nucleospin® Plant II kit and quantified using Qubit 2.0, Nanodrop and 1% agarose gel electrophoresis. The Ion Plus Fragment Library kit was used for the preparation of a 600 bp library, which was sequenced in two separate runs through the Ion S5 platform. The produced reads were quality-controlled through the Ion Torrent server version 5.6. and assessed using the FASTQC program. The SPAdes version 3.11.1 assembler was used to assemble the quality-controlled reads, and the resultant genome assembly was quality-assessed using the QUAST 4.1 software. The mitochondrial genome was selected from the produced Gelidium pristoides draft genome using mitochondrial genomes of other Gelidiales as search queries on the local BLAST algorithm of the BioEdit software. Contigs matching the organellar genomes were ordered according to the mitochondrial genomes of other Gelidiales using the trial version of Geneious R11.12 software. The plastid genome was also selected following the same approach but using plastid genomes of Gelidium elegans and Gelidium vagum as search queries. Gaps observed in the organellar genomes were closed by amplification of the relevant gap using polymerase chain reaction with newly designed primers and Sanger sequencing. Open reading frames for both organellar genomes were annotated using the NCBI ORF-Finder and alignments obtained from BlastN and BlastX searches from the NCBI database, while the tRNAs and rRNAs were identified using the tRNAscan-SE1.21 vi and the RNAmmer 1.2 servers. The circular physical map of the mitochondrial genome was constructed using the CGView server. Lastly, in silico analysis of cytochrome c oxidase 3 and Heat Shock Protein 70 was performed using the PRIMO and the SWISS-MODEL pipelines respectively. Their phylogenies were analysed through Clustal omega and the trees viewed on TreeView 1.6.6 software. Qubit and Nanodrop genomic DNA qualification revealed A260/A280 and A230/A260 ratios of 1.81 and 1.52 respectively. The 1% agarose gel electrophoresis further confirmed the good quality of the genomic DNA used for library preparation and sequencing. Pre-assembly quality control of reads resulted in a total of 30 792 074 high-quality reads which were assembled into a total of 94140 contigs, making up an estimated genome length of 217.06 Mb. The largest contig covered up to 13.17 kb of the draft genome, and an N50 statistic value of 3.17 kb was obtained. The G.pristoides mitochondrial genome mapped into a circular molecule of 25012 bp, with an overall GC content of 31.04% and a total of 45 genes distributed into 20 tRNA-coding, 2 rRNAcoding genes and 23 protein-coding genes, mostly adopting the modified genetic code of Rhodophyta. The SecY and rps12 genes overlapped by 41 bp. This study presents a partial plastid genome composed of 89 (38%) fully annotated genes, of which 71 are protein-coding, and 18 are distributed among 15 tRNA-coding, 2 rRNA-coding and 1 RNaseP RNA-coding genes. Sixty-one (26%) partial protein-coding genes were predicted, while approximately 84 (36%) genes are not yet predicted. In silico analysis of the cytochrome c oxidase and heat shock protein 70 showed that the gene sequences obtained in this study and the resultant transcribed protein have sequences and structures that are similar to those from several other different species, thus validating the integrity of the genome sequences. This study provides genomic data necessary for understanding the genomic constituent of G.pristoides and serve as a foundation for studies of individual genes and for resolving evolutionary relationships.
- Full Text:
- Date Issued: 2019
- Authors: Mangali, Sandisiwe
- Date: 2019
- Subjects: Gelidium -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/19109 , vital:39883
- Description: The genome is the complete set of an organism's hereditary information that contains all the information necessary for the functioning of that organism. Complete nuclear, mitochondrial and plastid DNA constitute the three main types of genomes which play interconnected roles in an organism. Genome sequencing enables researchers to understand the regulation and expression of the various genes and the proteins they encode. It allows researchers to extract and analyse genes of interests for a variety of studies including molecular, biotechnological, bioinformatics and conservation and evolutionary studies. Genome sequencing of Rhodophyta has received little attention. To date, no published studies are focusing on both whole genome sequencing and sequencing of the organellar genomes of Rhodophyta species found in along the South African coastline. This study focused on genome sequencing, assembly and annotation mitochondrial and plastid genomes of Gelidium pristoides. Gelidium pristoides was collected from Kenton-on-Sea and was morphologically identified at Rhodes University. Its genomic DNA was extracted using the Nucleospin® Plant II kit and quantified using Qubit 2.0, Nanodrop and 1% agarose gel electrophoresis. The Ion Plus Fragment Library kit was used for the preparation of a 600 bp library, which was sequenced in two separate runs through the Ion S5 platform. The produced reads were quality-controlled through the Ion Torrent server version 5.6. and assessed using the FASTQC program. The SPAdes version 3.11.1 assembler was used to assemble the quality-controlled reads, and the resultant genome assembly was quality-assessed using the QUAST 4.1 software. The mitochondrial genome was selected from the produced Gelidium pristoides draft genome using mitochondrial genomes of other Gelidiales as search queries on the local BLAST algorithm of the BioEdit software. Contigs matching the organellar genomes were ordered according to the mitochondrial genomes of other Gelidiales using the trial version of Geneious R11.12 software. The plastid genome was also selected following the same approach but using plastid genomes of Gelidium elegans and Gelidium vagum as search queries. Gaps observed in the organellar genomes were closed by amplification of the relevant gap using polymerase chain reaction with newly designed primers and Sanger sequencing. Open reading frames for both organellar genomes were annotated using the NCBI ORF-Finder and alignments obtained from BlastN and BlastX searches from the NCBI database, while the tRNAs and rRNAs were identified using the tRNAscan-SE1.21 vi and the RNAmmer 1.2 servers. The circular physical map of the mitochondrial genome was constructed using the CGView server. Lastly, in silico analysis of cytochrome c oxidase 3 and Heat Shock Protein 70 was performed using the PRIMO and the SWISS-MODEL pipelines respectively. Their phylogenies were analysed through Clustal omega and the trees viewed on TreeView 1.6.6 software. Qubit and Nanodrop genomic DNA qualification revealed A260/A280 and A230/A260 ratios of 1.81 and 1.52 respectively. The 1% agarose gel electrophoresis further confirmed the good quality of the genomic DNA used for library preparation and sequencing. Pre-assembly quality control of reads resulted in a total of 30 792 074 high-quality reads which were assembled into a total of 94140 contigs, making up an estimated genome length of 217.06 Mb. The largest contig covered up to 13.17 kb of the draft genome, and an N50 statistic value of 3.17 kb was obtained. The G.pristoides mitochondrial genome mapped into a circular molecule of 25012 bp, with an overall GC content of 31.04% and a total of 45 genes distributed into 20 tRNA-coding, 2 rRNAcoding genes and 23 protein-coding genes, mostly adopting the modified genetic code of Rhodophyta. The SecY and rps12 genes overlapped by 41 bp. This study presents a partial plastid genome composed of 89 (38%) fully annotated genes, of which 71 are protein-coding, and 18 are distributed among 15 tRNA-coding, 2 rRNA-coding and 1 RNaseP RNA-coding genes. Sixty-one (26%) partial protein-coding genes were predicted, while approximately 84 (36%) genes are not yet predicted. In silico analysis of the cytochrome c oxidase and heat shock protein 70 showed that the gene sequences obtained in this study and the resultant transcribed protein have sequences and structures that are similar to those from several other different species, thus validating the integrity of the genome sequences. This study provides genomic data necessary for understanding the genomic constituent of G.pristoides and serve as a foundation for studies of individual genes and for resolving evolutionary relationships.
- Full Text:
- Date Issued: 2019
The impact of public libraries in the Eastern Cape Province with reference to three Buffalo City Municipal libraries in King Williams' Town
- Authors: Gunuza, Nokuthula
- Date: 2019
- Subjects: Public libraries Information services
- Language: English
- Type: Thesis , Masters , M.Bibl
- Identifier: http://hdl.handle.net/10353/16452 , vital:40721
- Description: The study sought to investigate the impact of public libraries in developing information literacy in libraries in the Eastern Cape Province of South Africa: a case study of Buffalo City Municipality public libraries in King William’s Town. The objectives of the study were to find out how these three libraries in Buffalo City Metro public libraries ensure equity of library and information services provision to the user communities, to find out how public libraries support education and lifelong learning, to identify the challenges that public libraries face in rendering library and information services with reference to the three libraries, and to investigate the role of library professionals in marketing the library services to the community. Both quantitative and qualitative research methodologies were employed in a descriptive survey. The main research instrument was a questionnaire supported by face to face interviews. A total of 297 questionnaires were distributed and 200 were returned giving a response rate of 67.3%. The quantitative data which was collected through questionnaire was presented using graphs and tables, while qualitative was analysed manually by content analysis, using the notes that were taken by the researcher from the respondents during the interview sessions and reported verbatim in some instances. The findings showed that regardless of South African LIS environment having contradictions resulting from years of apartheid, most of the Buffalo City Metro public library patrons indicated that they experienced fair and equitable access to library and information services. In addition to participants’ quantitative responses, the qualitative excerpts from librarians attested to the library having a classification system that allows easy access to library resources. In addition, findings of the study also revealed that the Buffalo City Metro public libraries have a diversity of users, ranging from different age groups and social standing. The results from the current study revealed that the Buffalo City metropolitan public library users have little knowledge about literacy programmes that are taking place in the library. The findings also indicated that the Buffalo City Metro public libraries market library services by word of mouth, posters and notice boards. One of the major findings of the study is that Buffalo City Metro public libraries are faced with financial constraints and that led to a myriad of challenges ranging from inadequate library operating hours; limited information sources; lack of information retrieval training to patrons; internet connectivity issues; xii lack of professional staff and lack of training on the latest technological trends. The study concludes that the Buffalo City Metro public libraries should establish a marketing strategy to motivate for funding. The study recommended that the Buffalo City public libraries should create an integrated system for all libraries in the metromunicipality that encourages adult literacy programme to raise awareness of information literacy.
- Full Text:
- Date Issued: 2019
- Authors: Gunuza, Nokuthula
- Date: 2019
- Subjects: Public libraries Information services
- Language: English
- Type: Thesis , Masters , M.Bibl
- Identifier: http://hdl.handle.net/10353/16452 , vital:40721
- Description: The study sought to investigate the impact of public libraries in developing information literacy in libraries in the Eastern Cape Province of South Africa: a case study of Buffalo City Municipality public libraries in King William’s Town. The objectives of the study were to find out how these three libraries in Buffalo City Metro public libraries ensure equity of library and information services provision to the user communities, to find out how public libraries support education and lifelong learning, to identify the challenges that public libraries face in rendering library and information services with reference to the three libraries, and to investigate the role of library professionals in marketing the library services to the community. Both quantitative and qualitative research methodologies were employed in a descriptive survey. The main research instrument was a questionnaire supported by face to face interviews. A total of 297 questionnaires were distributed and 200 were returned giving a response rate of 67.3%. The quantitative data which was collected through questionnaire was presented using graphs and tables, while qualitative was analysed manually by content analysis, using the notes that were taken by the researcher from the respondents during the interview sessions and reported verbatim in some instances. The findings showed that regardless of South African LIS environment having contradictions resulting from years of apartheid, most of the Buffalo City Metro public library patrons indicated that they experienced fair and equitable access to library and information services. In addition to participants’ quantitative responses, the qualitative excerpts from librarians attested to the library having a classification system that allows easy access to library resources. In addition, findings of the study also revealed that the Buffalo City Metro public libraries have a diversity of users, ranging from different age groups and social standing. The results from the current study revealed that the Buffalo City metropolitan public library users have little knowledge about literacy programmes that are taking place in the library. The findings also indicated that the Buffalo City Metro public libraries market library services by word of mouth, posters and notice boards. One of the major findings of the study is that Buffalo City Metro public libraries are faced with financial constraints and that led to a myriad of challenges ranging from inadequate library operating hours; limited information sources; lack of information retrieval training to patrons; internet connectivity issues; xii lack of professional staff and lack of training on the latest technological trends. The study concludes that the Buffalo City Metro public libraries should establish a marketing strategy to motivate for funding. The study recommended that the Buffalo City public libraries should create an integrated system for all libraries in the metromunicipality that encourages adult literacy programme to raise awareness of information literacy.
- Full Text:
- Date Issued: 2019
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