Characterization of arbuscular mycorrhizal fungal species associating with Zea mays

Maússe-Sitoe, Silvia; Dames, Joanna F

Title: Characterization of arbuscular mycorrhizal fungal species associating with Zea mays
Creator: Maússe-Sitoe, Silvia
Creator: Dames, Joanna F
Subject: To be catalogued
Date Issued: 2024
Date: 2024
Type: text
Type: article
Identifier: http://hdl.handle.net/10962/440339
Identifier: vital:73775
Identifier: https://doi.org/10.3389/fpls.2024.1345229
Description: Taxonomic identification of arbuscular mycorrhizal (AM) fungal spores extracted directly from the field is sometimes difficult because spores are often degraded or parasitized by other organisms. Single-spore inoculation of a suitable host plant allows for establishing monosporic cultures of AM fungi. This study aimed to propagate AM fungal spores isolated from maize soil using single spores for morphological characterization. First, trap cultures were established to trigger the sporulation of AM fungal species. Second, trap cultures were established with individual morphotypes by picking up only one spore under a dissecting microscope and transferring it to a small triangle of sterilized filter paper, which was then carefully inoculated below a root from germinated sorghum seeds in each pot and covered with a sterile substrate. All pots were placed in sunbags and maintained in a plant growth room for 120 days.
Format: 16 pages
Format: pdf
Language: English
Relation: Frontiers in Plant Science
Relation: Maússe-Sitoe, S. and Dames, J., 2024. Characterization of arbuscular mycorrhizal fungal species associating with Zea mays. Frontiers in Plant Science, 15, p.1345229
Relation: Frontiers in Plant Science volume 15 number 1 1 16 2024
Rights: Publisher
Rights: Use of this resource is governed by the terms and conditions of the Frontiers Terms and Conditions Statement (https://www.frontiersin.org/legal/terms-and-conditions)

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Dames, Joanna Felicity (Prof)

RU Department of Biochemistry, Microbiology and Bioinformatics

SDG 15. Life On Land

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