- Title
- The antibody assisted detection of HER2 on a cobalt porphyrin binuclear framework and gold functionalized graphene quantum dots modified electrode
- Creator
- Centane, Sixole
- Creator
- Nyokong, Tebello
- Subject
- To be catalogued
- Date Issued
- 2021
- Date
- 2021
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/185931
- Identifier
- vital:44449
- Identifier
- xlink:href="https://doi.org/10.1016/j.jelechem.2020.114908"
- Description
- In this work a novel simple, inexpensive and highly sensitive approach for the antibody assisted detection of the human epidermal growth factor receptor 2 (HER2) is presented. Three sensors using carboxylic group containing gold functionalized graphene quantum dots (SNGQDs@AuNPs), CoP-BNF (cobalt porphyrin binuclear framework) and CoP-BNF/SNGQDs@AuNPs (the conjugate) nanocomposites on a GCE were designed. The sensors were fabricated by covalent immobilization of the antibody to the GCE via an amide bond. The modified GCE was characterized using cyclic voltammetry and impedance spectroscopy. The performance of the sensors was assessed using electrochemical impedance spectroscopy. The selective recognition of the antibody towards HER2 on the electrode led to an increase of the electron transfer resistance (Rct). The different modified electrodes had low detection limit values: 0.0327 ng/mL, 0.0454 ng/mL and 0.1072 ng/mL towards the detection of HER2. The sensors showed good stability and reproducibility. The sensors showed promising potential in clinical applicability, in the detection of HER2 protein in human serum.
- Format
- computer
- Format
- online resource
- Format
- application/pdf
- Format
- 1 online resource (12 pages)
- Format
- Publisher
- Elsevier
- Language
- English
- Relation
- Journal of Electroanalytical Chemistry
- Relation
- Centane, S. and Nyokong, T., 2021. The antibody assisted detection of HER2 on a cobalt porphyrin binuclear framework and gold functionalized graphene quantum dots modified electrode. Journal of Electroanalytical Chemistry, 880, p.114908
- Relation
- Journal of Electroanalytical Chemistry volume 880 p. 114908 2021 1572-6657
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Elsevier Terms and Conditions Statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)
- Rights
- Closed Access
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RU DST/NRF SARChI for Medicinal Chemistry and Nanotechnology
Nyokong, Tebello (Distinguished Professor)
RU Department of Chemistry
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | The antibody assisted detection of HER2.pdf | 778 KB | Adobe Acrobat PDF | View Details Download |