- Title
- Simultaneous determination of irinotecan hydrochloride and its related compounds by high performance liquid chromatography using ultraviolet detection
- Creator
- Mohammadi, Ali
- Creator
- Esmaeili, Farnaz
- Creator
- Dinarvand, Rassoul
- Creator
- Atyabi, Fatemeh
- Creator
- Walker, Roderick B
- Date Issued
- 2010
- Date
- 2010
- Type
- Article
- Identifier
- vital:6413
- Identifier
- http://hdl.handle.net/10962/d1006508
- Description
- A new simple, precise and accurate high performance liquid chromatography (HPLC) method was developed and validated for the simultaneous determination of irinotecan (CPT-11) and two related compounds viz., 7-ethyl-10 hydroxycamptothecin (SN-38) and camptothecin (CPT) in pharmaceutical dosage forms. Chromatography was accomplished using a reversed-phase C18 column and ultraviolet (UV)detection and an isocratic mobile phase consisting of 3 % v/v triethylammonium acetate buffer (pH 3) and acetonitrile (70:30 v/v). The linear range of quantitation for all the compounds was 0.1-10 μg/mL. The limit of quantitation for all the compounds ranged between 0.01-0.05 μg/mL. The method has the requisite accuracy, selectivity, sensitivity and precision to assay of CPT-11 and related compounds in pharmaceutical dosage forms and bulk API.
- Format
- 7 pages
- Format
- Language
- English
- Relation
- Mohammadi, A. and Esmaeili, F and Dinarvand, R. and Atyabi, F and Walker, R.B. (2010) Simultaneous determination of irinotecan hydrochloride and its related compounds by high performance liquid chromatography using ultraviolet detection. Asian Journal of Chemistry, 22 (5). pp. 3966-3972
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Walker, Roderick Bryan (Prof)
RU Department of Pharmacy
SDG 03. Good Health and Well-Being
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