- Title
- Comparison of fluorophore and peroxidase labeled aptamer assays for MUC1 detection in cancer cells
- Creator
- Flanagan, Shane
- Creator
- Limson, Janice
- Creator
- Fogel, Ronen
- Subject
- To be catalogued
- Date Issued
- 2014
- Date
- 2014
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/431076
- Identifier
- vital:72742
- Identifier
- xlink:href="10.1109/BioCAS.2014.6981720"
- Description
- Aptamers hold great promise for cancer diagnosis and therapy. Several biosensors incorporate aptamers as biorecognition elements for tumor markers although few evaluate their detection in a native conformation and cellular micro-environment. In this study, fluorophore and peroxidase labeled aptamer configurations were compared for the detection of MCF7 breast and SW620 colon cancer cell lines expressing the tumor marker MUC1. Fluorescence based detection showed selective binding to the cell lines relative to a nonbinding control sequence with sequence specific binding differences between MUC1 aptamers accredited to variation in the glycosylation state of expressed MUC1. The peroxidase labeled assay showed high detection sensitivity although low binding specificity was observed for the MUC1 aptamers to the cell lines. Results suggest that aptamers susceptible to non specific binding to cells may limit the applicability of enzymatic amplification to improve aptasensor sensitivity.
- Format
- computer
- Format
- online resource
- Format
- application/pdf
- Format
- 1 online resource (3 pages)
- Format
- Publisher
- IEEE Xplore
- Language
- English
- Relation
- IEEE Biomedical Circuits and Systems Conference (BioCAS) Proceedings
- Relation
- Flanagan, S., Limson, J. and Fogel, R., 2014, October. Comparison of fluorophore and peroxidase labeled aptamer assays for MUC1 detection in cancer cells. In 2014 IEEE Biomedical Circuits and Systems Conference (BioCAS) Proceedings (pp. 292-295). IEEE
- Relation
- IEEE Biomedical Circuits and Systems Conference (BioCAS) Proceedings p. 292 2014 1873-1902
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the IEEE Xplore Terms of Use Statement (https://ieeexplore.ieee.org/Xplorehelp/overview-of-ieee-xplore/terms-of-use)
- Rights
- Closed Access
- Hits: 106
- Visitors: 108
- Downloads: 4
Collections
Fogel, Ronen (Dr)
Limson, Janice (Prof)
RU DST/NRF SARChI for Biotechnology Innovation and Engagement
RU Department of Biochemistry, Microbiology and Bioinformatics
SDG 03. Good Health and Well-Being
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | Comparison of fluorophore and peroxidase labeled aptamer assays for MUC1 detection in cancer cells.pdf | 697 KB | Adobe Acrobat PDF | View Details Download |