- Title
- Quality indices of the Eastern Cape beach waters in Buffalo City Metropolitan Municipality, Eastern Cape, South Africa
- Creator
- Mani, Sinalo
- Subject
- Waterfronts -- Recreational use -- South Africa -- Eastern Cape Water quality -- South Africa -- Eastern Cape
- Date Issued
- 2017
- Date
- 2017
- Type
- Thesis
- Type
- Masters
- Type
- MSc
- Identifier
- http://hdl.handle.net/10353/19054
- Identifier
- vital:40108
- Description
- Surface waters constitute important sources of water and serve for agricultural, recreational and industrial purposes; however, the vulnerability of such water sources to pollution presents serious ecological, public health and economic problems of global concerns. Beach water quality is monitored to reduce the risk of recreational waterborne illnesses. Water samples were collected between September 2014 and August 2015 at Eastern beach and a total of 5 physicochemical parameters, counts of faecal coliforms, E. coli and Enterococcus isolates were determined using standard analytical procedures. Confirmed Escherichia coli isolates were assessed for virulence genes associated with pathogenic E. coli strains responsible for intestinal and extra-intestinal infections. Recovered E. coli isolates were then interpreted for their antibiogram profiling using disk diffusion method. On the other hand, the recovered Enterococcus isolates were characterized for Enterococcus speciation of key species that are normally implicated in infections and further assessed for their antibiogram profiling by disk diffusion method. The physicochemical qualities of the water ranged as follows: turbidity (1.4 to 36.8 NTU); pH (7.0 to 8.6); temperature (14.5 to 24.5 ºC); total dissolved solids (TDS) (5.2 to 46 mg/L) and electrical conductivity (EC) (8.2 to 70.7 μS/cm). Microbiological qualities obtained were as follows: faecal coliform (101 to 103 CFU/100 mL); Escherichia coli (101 to 103 CFU/100 mL); and enterococci (101 CFU/100 mL to 102 CFU/100 mL). Only 38/104 (37 percent) harbored at least 1 virulence gene while 66/104 (63 percent) of the isolates harbored none. Most frequent virulence genes were eae for EPEC/EHEC, fliCH7 for EHEC, ibeA for NMEC, while daaE for DAEC and ipaH for EIEC were discovered in three isolates each; bfp for EPEC was detected in 2 isolates and lt gene for ETEC in one isolate. EAEC and UPEC genes were not detected in all the isolates. All tested isolates were resistant to ampicillin, tetracycline, gentamycin and chloramphenicol (85 percent), ciprofloxacin (78 percent), trimethoprim (54 percent) and norfloxacin (54 percent) and the Multiple Antibiotic Resistance Index for all isolates ranged between 0.6- 1.0 with the average being 0.8, which exceeded the threshold value of 0.2. Among the 96 recovered Enterococcus isolates, 55 percent were identified as E. faecium, 22 percent were E. hirae, 2 percent were E. durans and 21 percent were uncharacterized Enterococcus species. E. faecalis and E. casseliflavus were not detected amongst the isolates. All isolates were resistant to vancomycin, erythromycin and chloramphenicol. While the resistance against other antibiotics followed the order: tetracycline (97 percent), gentamycin (96 percent), ciprofloxacin (93 percent), linezolid (91 percent), ampicillin (91 percent) and trimethoprim (62percent). Only 6 percent of the isolates were susceptible to trimethoprim and the MAR index for all isolates ranged between 0.6- 1.0 with the average being 0.9 which greater than 0.2, suggesting that these bacterial strains originates from environments with high use or contamination of antibiotics.
- Format
- 133 leaves
- Format
- Publisher
- University of Fort Hare
- Publisher
- Faculty of Science and Agriculture
- Language
- English
- Rights
- University of Fort Hare
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UFH Department of Biochemistry & Microbiology
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | MSc (Microbiology) MANI, S.pdf | 1 MB | Adobe Acrobat PDF | View Details Download |