Evidence of genetic differentiation in cigar wrasse Cheilio inermis (Labridae) within the western Indian Ocean:
- Mayekiso, Sisanda, Gouws, Gavin, Mwale, Monica, Gon, Ofer
- Authors: Mayekiso, Sisanda , Gouws, Gavin , Mwale, Monica , Gon, Ofer
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/163499 , vital:41043 , https://doi.org/10.1139/gen-2019-0185
- Description: Patterns of genetic structure and connectivity of the monotypic cigar wrasse Cheilio inermis within western Indian Ocean (WIO) are poorly understood. Whether the species exists as a single panmictic population across the WIO is unclear. Sequence data were generated from two mitochondrial genes (cytochrome b and ATPase 6) and one nuclear intron (S7 intron I). High levels of haplotype and allelic diversity (h = 0.88–0.98; A = 0.95–0.98), along with low nucleotide diversities were observed across all markers.
- Full Text:
- Date Issued: 2020
- Authors: Mayekiso, Sisanda , Gouws, Gavin , Mwale, Monica , Gon, Ofer
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/163499 , vital:41043 , https://doi.org/10.1139/gen-2019-0185
- Description: Patterns of genetic structure and connectivity of the monotypic cigar wrasse Cheilio inermis within western Indian Ocean (WIO) are poorly understood. Whether the species exists as a single panmictic population across the WIO is unclear. Sequence data were generated from two mitochondrial genes (cytochrome b and ATPase 6) and one nuclear intron (S7 intron I). High levels of haplotype and allelic diversity (h = 0.88–0.98; A = 0.95–0.98), along with low nucleotide diversities were observed across all markers.
- Full Text:
- Date Issued: 2020
Identification of a uniquely southern African clade of coastal pipefishes Syngnathus spp.
- Mwale, Monica, Kaiser, Horst, Barker, Nigel P, Wilson, A B, Teske, Peter R
- Authors: Mwale, Monica , Kaiser, Horst , Barker, Nigel P , Wilson, A B , Teske, Peter R
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/445499 , vital:74393 , https://doi.org/10.1111/jfb.12130
- Description: The taxonomic status of two southern African coastal pipefish species, Syngnathus temminckii and Syngnathus watermeyeri, was investigated using a combination of morphological and genetic data. Morphological data showed that S. temminckii is distinct from the broadly distributed European pipefish Syngnathus acus, and a molecular phylogeny reconstructed using mitochondrial DNA recovered S. temminckii and S. watermeyeri as sister taxa. The southern African species share an evolutionary origin with north‐eastern Atlantic Ocean and Mediterranean Sea species, including S. acus. These data support the existence of a distinct southern African clade of Syngnathus pipefishes that has diverged in situ to form the two species present in the region today.
- Full Text:
- Date Issued: 2013
- Authors: Mwale, Monica , Kaiser, Horst , Barker, Nigel P , Wilson, A B , Teske, Peter R
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/445499 , vital:74393 , https://doi.org/10.1111/jfb.12130
- Description: The taxonomic status of two southern African coastal pipefish species, Syngnathus temminckii and Syngnathus watermeyeri, was investigated using a combination of morphological and genetic data. Morphological data showed that S. temminckii is distinct from the broadly distributed European pipefish Syngnathus acus, and a molecular phylogeny reconstructed using mitochondrial DNA recovered S. temminckii and S. watermeyeri as sister taxa. The southern African species share an evolutionary origin with north‐eastern Atlantic Ocean and Mediterranean Sea species, including S. acus. These data support the existence of a distinct southern African clade of Syngnathus pipefishes that has diverged in situ to form the two species present in the region today.
- Full Text:
- Date Issued: 2013
The biology and systematics of South African pipefishes of the genus Syngnathus
- Authors: Mwale, Monica
- Date: 2007
- Subjects: Pipefishes -- South Africa Syngnathus -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5237 , http://hdl.handle.net/10962/d1005080
- Description: Syngnathus the most speciose genus in the family Syngnathidae is widely in the Atlantic and Eastern Pacific oceans. However, it is poorly represented in the Indian Ocean with the only two species, Syngnathus temminckii and S. watermeyeri occurring in Southern African estuaries and coastal areas. Syngnathus temminckii the most common South African pipefish has been synonymised with S. acus, as the morphological and genetic divergence between these two populations has not been documented. There is also uncertainty in the taxonomic status of S. watermeyeri, an endemic estuarine pipefish that is restricted to two Eastern Cape estuaries. The purpose of this study was therefore to compare biological, morphological and genetic variation of South African Syngnathus species among different populations/locations, and with European populations of S. acus. Sixteen meristic and ten morphometric characteristics were quantified from specimens obtained from field as well as various international natural history museum collections. Univariate (ANOVA) and multivariate (principal component analysis and discriminant analyses) analyses were used to assess morphological differences among the species. Morphometric variables were adjusted as ratios of the standard length and using an allometric procedure. ANCOVA analysis indicated significant differences between S. acus and S. temminckii for the relationships of the standard length (SL) and all morphological characters. There was no significant correlation between SL and snout length, snout depth, inter-orbital width and trunk depth for S. watermeyeri. The analyses provided evidence for distinct populations of S. acus, S. temminckii and S. watermeyeri although morphological character differentiation was greater between S. watermeyeri and the other two larger species. Although, significant differences were observed for meristic characters, pairwise comparisons did not reflect a clear pattern of variability. Most of the measured morphological characters contributed more than 70% to the morphological variation between the populations. Plot of the canonical scores for the variables resulted in the specimens clustering according to species groups and locations of S. temminckii. Sequences of 750 base pairs of the mitochondrial cytochrome gene from 11 localities were compared with published sequences of other species of Syngnathus. Phylogenetic analysis was performed using parsimony, maximum likelihood (ML) and Bayesian inference (BI). The South African species were revealed to be sister-taxa with about 6 % divergence, while S. temminckii and S. acus had about 11% sequence divergence. 20 haplotypes among 46 total specimens from the three species. Gene flow was estimated at approximately 3 migrants per generation between the two South African populations and about 1 per generation between S. temminckii and S. acus. Such strong stock structuring among presumably recently established post-Pliocene (< 2 Million years ago) populations suggests that these species are reproductively isolated. Morphological and genetic variation observed in this study combined with current knowledge of life history attributes of the South African pipefishes indicate that conservative management decisions are necessary until the patterns and extent of differentiation among populations species-wide can be investigated further. It is thus being proposed that the name of the South African population of S. acus be changed to Syngnathus temminckii (Kaup, 1856).
- Full Text:
- Date Issued: 2007
- Authors: Mwale, Monica
- Date: 2007
- Subjects: Pipefishes -- South Africa Syngnathus -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5237 , http://hdl.handle.net/10962/d1005080
- Description: Syngnathus the most speciose genus in the family Syngnathidae is widely in the Atlantic and Eastern Pacific oceans. However, it is poorly represented in the Indian Ocean with the only two species, Syngnathus temminckii and S. watermeyeri occurring in Southern African estuaries and coastal areas. Syngnathus temminckii the most common South African pipefish has been synonymised with S. acus, as the morphological and genetic divergence between these two populations has not been documented. There is also uncertainty in the taxonomic status of S. watermeyeri, an endemic estuarine pipefish that is restricted to two Eastern Cape estuaries. The purpose of this study was therefore to compare biological, morphological and genetic variation of South African Syngnathus species among different populations/locations, and with European populations of S. acus. Sixteen meristic and ten morphometric characteristics were quantified from specimens obtained from field as well as various international natural history museum collections. Univariate (ANOVA) and multivariate (principal component analysis and discriminant analyses) analyses were used to assess morphological differences among the species. Morphometric variables were adjusted as ratios of the standard length and using an allometric procedure. ANCOVA analysis indicated significant differences between S. acus and S. temminckii for the relationships of the standard length (SL) and all morphological characters. There was no significant correlation between SL and snout length, snout depth, inter-orbital width and trunk depth for S. watermeyeri. The analyses provided evidence for distinct populations of S. acus, S. temminckii and S. watermeyeri although morphological character differentiation was greater between S. watermeyeri and the other two larger species. Although, significant differences were observed for meristic characters, pairwise comparisons did not reflect a clear pattern of variability. Most of the measured morphological characters contributed more than 70% to the morphological variation between the populations. Plot of the canonical scores for the variables resulted in the specimens clustering according to species groups and locations of S. temminckii. Sequences of 750 base pairs of the mitochondrial cytochrome gene from 11 localities were compared with published sequences of other species of Syngnathus. Phylogenetic analysis was performed using parsimony, maximum likelihood (ML) and Bayesian inference (BI). The South African species were revealed to be sister-taxa with about 6 % divergence, while S. temminckii and S. acus had about 11% sequence divergence. 20 haplotypes among 46 total specimens from the three species. Gene flow was estimated at approximately 3 migrants per generation between the two South African populations and about 1 per generation between S. temminckii and S. acus. Such strong stock structuring among presumably recently established post-Pliocene (< 2 Million years ago) populations suggests that these species are reproductively isolated. Morphological and genetic variation observed in this study combined with current knowledge of life history attributes of the South African pipefishes indicate that conservative management decisions are necessary until the patterns and extent of differentiation among populations species-wide can be investigated further. It is thus being proposed that the name of the South African population of S. acus be changed to Syngnathus temminckii (Kaup, 1856).
- Full Text:
- Date Issued: 2007
Ammonia removal from water by ion exchange using South African and Zambian zeolite samples
- Authors: Mwale, Monica
- Date: 2000
- Subjects: Ammonia -- Toxicology , Water -- Purification , Zeolites , Ion exchange
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5215 , http://hdl.handle.net/10962/d1005058 , Ammonia -- Toxicology , Water -- Purification , Zeolites , Ion exchange
- Description: One problem of intensive fish culture systems is the progressive build-up of toxic wastes such as ammonia. The possibility of improving aquaculture water quality using two kinds of zeolite is discussed. Zeolites are alumino-silicates whose framework allows them to exchange cations. Ion exchange has been demonstrated to be competitive with other methods of ammonia removal due to the high selectivity for ammonia exhibited by zeolite materials. In this study an unknown Zambian zeolite (identified as laumontite by X-ray diffraction techniques) and Pratley clinoptilolite (a South African zeolite) were tested under laboratory conditions and in a fresh water recirculating system. Ammonia cation exchange capacities (CEC) and suitable application rates for efficient water treatment were determined using the batch and column ion exchange procedures. Estimated ammonia uptake, the most important criterion used to assess performance of zeolite filters was strongly influenced by zeolite type, particle size, pre-treatment, regeneration and ion exchange method used. Statistical analysis showed significant differences in average ammonia CEC values between clinoptilolite (14.94 mg g⁻¹) and laumontite (2.77 mg g⁻¹), with the former displaying a higher Na⁺ ® NH₄⁺ exchange rate especially in the early reaction stages. This difference accords with the higher purity of clinoptilolite, 47% as opposed to 4.7% for laumontite, which makes it a better zeolite for ammonium removal. CEC increased linearly as particle size of the clinoptilolite was reduced resulting in a linear regression model (y = 18.29 – 3.704 x; r² = 74%). Pre-treatment of clinoptilolite using 1N NaCl significantly improved the ammonia CEC of clinoptilolite. Overall performance of both the batch and column methods achieved after regeneration (18.3 mg g⁻¹) was 25% higher than the estimated CEC values (13.0 mg g⁻¹) for the unregenerated samples of clinoptilolite. Comparison of CEC estimates using Pratley clinoptilolite, showed that average batch CEC estimates were significantly lower than the column method estimates. The average ammonia CEC values estimated in a fresh water recirculating system (5.80 mg g⁻¹ and 4.12 mg g⁻¹ for the 0.7-1.0 and 1.0-1.4 mm particle sizes, respectively) were significantly lower than the column and batch estimates for the same particle sizes (P < 0.05). Some nitrite (NO₂) and nitrate (NO3) build up was experienced probably due to the growth of autotrophs in the filters. Mass balance of nitrogen (N) for the three treatments of the fish trial (0.7-1.0 mm, 1.0-1.4 mm and the control treatment that had no zeolite in the filter) indicated that less that 10% of the N was retained for growth. It was found that 60% of the NH₄-N present associated with the soluble N was available for absorption by the zeolite filter or biological nitrification and that a total of approximately 22% of NH₄-N available was absorbed by clinoptilolite. The results indicate that the rate of nitrification can be deductively estimated by allowing a zeolite filter to become a biological filter. It is concluded that water treatment by ion exchange using natural zeolites, provides a reliable and efficient method for ammonia removal and appears to be a viable supplementary water treatment method for fresh water systems.
- Full Text:
- Date Issued: 2000
- Authors: Mwale, Monica
- Date: 2000
- Subjects: Ammonia -- Toxicology , Water -- Purification , Zeolites , Ion exchange
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5215 , http://hdl.handle.net/10962/d1005058 , Ammonia -- Toxicology , Water -- Purification , Zeolites , Ion exchange
- Description: One problem of intensive fish culture systems is the progressive build-up of toxic wastes such as ammonia. The possibility of improving aquaculture water quality using two kinds of zeolite is discussed. Zeolites are alumino-silicates whose framework allows them to exchange cations. Ion exchange has been demonstrated to be competitive with other methods of ammonia removal due to the high selectivity for ammonia exhibited by zeolite materials. In this study an unknown Zambian zeolite (identified as laumontite by X-ray diffraction techniques) and Pratley clinoptilolite (a South African zeolite) were tested under laboratory conditions and in a fresh water recirculating system. Ammonia cation exchange capacities (CEC) and suitable application rates for efficient water treatment were determined using the batch and column ion exchange procedures. Estimated ammonia uptake, the most important criterion used to assess performance of zeolite filters was strongly influenced by zeolite type, particle size, pre-treatment, regeneration and ion exchange method used. Statistical analysis showed significant differences in average ammonia CEC values between clinoptilolite (14.94 mg g⁻¹) and laumontite (2.77 mg g⁻¹), with the former displaying a higher Na⁺ ® NH₄⁺ exchange rate especially in the early reaction stages. This difference accords with the higher purity of clinoptilolite, 47% as opposed to 4.7% for laumontite, which makes it a better zeolite for ammonium removal. CEC increased linearly as particle size of the clinoptilolite was reduced resulting in a linear regression model (y = 18.29 – 3.704 x; r² = 74%). Pre-treatment of clinoptilolite using 1N NaCl significantly improved the ammonia CEC of clinoptilolite. Overall performance of both the batch and column methods achieved after regeneration (18.3 mg g⁻¹) was 25% higher than the estimated CEC values (13.0 mg g⁻¹) for the unregenerated samples of clinoptilolite. Comparison of CEC estimates using Pratley clinoptilolite, showed that average batch CEC estimates were significantly lower than the column method estimates. The average ammonia CEC values estimated in a fresh water recirculating system (5.80 mg g⁻¹ and 4.12 mg g⁻¹ for the 0.7-1.0 and 1.0-1.4 mm particle sizes, respectively) were significantly lower than the column and batch estimates for the same particle sizes (P < 0.05). Some nitrite (NO₂) and nitrate (NO3) build up was experienced probably due to the growth of autotrophs in the filters. Mass balance of nitrogen (N) for the three treatments of the fish trial (0.7-1.0 mm, 1.0-1.4 mm and the control treatment that had no zeolite in the filter) indicated that less that 10% of the N was retained for growth. It was found that 60% of the NH₄-N present associated with the soluble N was available for absorption by the zeolite filter or biological nitrification and that a total of approximately 22% of NH₄-N available was absorbed by clinoptilolite. The results indicate that the rate of nitrification can be deductively estimated by allowing a zeolite filter to become a biological filter. It is concluded that water treatment by ion exchange using natural zeolites, provides a reliable and efficient method for ammonia removal and appears to be a viable supplementary water treatment method for fresh water systems.
- Full Text:
- Date Issued: 2000
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