Purification and characterization of β-mannanase from Aspergillus terreus and its applicability in depolymerization of mannans and saccharification of lignocellulosic biomass

Soni, Hemant; Rawat, Hemant Kumar; Kango, Naveen

Title: Purification and characterization of β-mannanase from Aspergillus terreus and its applicability in depolymerization of mannans and saccharification of lignocellulosic biomass
Creator: Soni, Hemant
Creator: Rawat, Hemant Kumar
Creator: Kango, Naveen
Date Issued: 2016
Date: 2016
Type: text
Type: article
Identifier: http://hdl.handle.net/10962/66167
Identifier: vital:28912
Identifier: https://doi.org/10.1007/s13205-016-0454-2
Description: publisher version
Description: Aspergillus terreus FBCC 1369 was grown in solid-state culture under statistically optimized conditions. β-Mannanase was purified to apparent homogeneity by ultrafiltration, anion exchange and gel filtration chromatography. A purification factor of 10.3-fold was achieved, with the purified enzyme exhibiting specific activity of 53 U/mg protein. The purified β-mannanase was optimally active at pH 7.0 and 70 °C and displayed stability over a broad pH range of 4.0–8.0 and a 30 min half-life at 80 °C. The molecular weight of β-mannanase was calculated as ~49 kDa by SDS-PAGE. The enzyme exhibited K m and V maxvalues of 5.9 mg/ml and 39.42 µmol/ml/min, respectively. β-Mannanase activity was stimulated by β-mercaptoethanol and strongly inhibited by Hg2+. The β-Mannanase did not hydrolyze mannobiose and mannotriose, but only mannotetraose liberating mannose and mannotriose. This indicated that at least four mannose residues were required for catalytic activity. Oligosaccharide with a degree of polymerization (DP) three was the predominant product in the case of locust bean gum (16.5 %) and guar gum (15.8 %) hydrolysis. However, the enzyme liberated DP4 oligosaccharide (24 %) exclusively from konjac gum. This property can be exploited in oligosaccharides production with DP 3–4. β-Mannanase hydrolyzed pretreated lignocelluloses and liberated reducing sugars (% theoretical yield) from copra meal (30 %). This property is an important factor for the bioconversion of the biomass.
Format: 11 pages
Format: pdf
Language: English
Relation: 3 Biotech
Relation: Soni, H., Rawat, H. K., Pletschke, B. I., and Kango, N. (2016). Purification and characterization of β-mannanase from Aspergillus terreus and its applicability in depolymerization of mannans and saccharification of lignocellulosic biomass. 3 Biotech, 6(2), 136. http://doi.org/10.1007/s13205-016-0454-2
Relation: 3 Biotech volume 6 number (2) 136 1 11 December 2016 2190-5738
Rights: Springer Nature
Rights: Use of this resource is governed by the terms and conditions of the 3 Biotech Open Access Statement (http://www.springer.com/?SGWID=0-0-1500-1593603-0_cm_mmc=AD-_-CFP-_-SOC15105_V1-_-0)

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Pletschke, Brett (Prof)

RU Department of Biochemistry, Microbiology and Bioinformatics

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