Bioprospectin of medicinal cannabis from Eastern Cape, South Africa
- Authors: Odieka, Anwuli, Endurance
- Date: 2024-02
- Subjects: Organic chemistry
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/11260/9427 , vital:73532
- Description: Cannabis sativa L. (Family Cannabaceae) has continued to attract much interest among natural product scientists in recent time because of its known medicinal values and remarkable chemical diversity. This study reports the investigation of C. sativa cultivated in the Eastern Cape Province of South Africa, with a view to obtaining its chemical fingerprint and isolating compounds from its stem bark for possible biological applications. Fresh and dried leaves, flowers, stem barks and roots of two Cannabis cultivars were obtained under permit NO. PIA-HP-EC-2022-0023. The fresh and dried plant materials were extracted for their essential oils (EOs), using hydro-distillation method on a Clevenger apparatus set at optimum extraction condition. The EOs were characterized by a gas chromatography coupled to a mass spectrometer (GC-MS). The chemical components of the EOs were elucidated based on their retention indices, as well as with reference to literature report. Other portions of the dried leaf, inflorescence and stem bark of each cultivar were extracted with methanol. The methanol extract was profiled for its cannabinoid content using liquid chromatography mass spectrometry (LCMS) based metabolomics approach, with reference to standard cannabinoids (∆9-THC, ∆9-THCA, and CBD). Lastly, the dried stem bark of Cultivar II was investigated for its compounds. The stem bark powder was subjected to sequential extraction at room temperature with n-hexane, dichloromethane, ethyl acetate and methanol. Based on the TLC analysis, the afforded n-hexane and ethyl acetate extracts were further individually fractionated on a repeated column chromatography, using silica gel and Sephadex LH20 as stationary phases. The column fractionation process led to the isolation of seven compounds, E1-E4, A20, A21, and A23. The isolated compounds were characterized by spectroscopic and spectrometric methods on nuclear magnetic resonance (NMR) spectrometer and ultra high-performance liquid chromatography hyphenated to a mass spectrometer, respectively. The results from the study showed that the EOs of the C. sativa cultivars contain majorly sesquiterpenes in their oxygenated and non-oxygenated forms. Caryophyllene (0.6421.4%), caryophyllene oxide (1.27-20.34%), -pinene (0.35-13.21%), D-limonene (0.51-10.99%), humulene (0.37-10.56%), humulene-1,2-epoxide (0.45-5.98%), pinene (0.16-5.22%), nerolidol (0.32-4.99%), -myrcene (0.31-4.23%), -selinene (0.92-2.51%), and trans--bergamotene (0.50-2.70%), were the main terpene constituents of the EOs in both cultivars. Neutral cannabinoids were also detected in both cultivars with cannabidiol found in significant amount up to 85.03% in the fresh stem oil (LSO) of Cultivar I (Lifter). LC-MS fingerprints revealed that Cultivar I had the highest accumulation of cannabinoids with CBDA having the highest amount (88.65%) in the stembark and CBD at about 23% in the leaves. The inflorescence (flower) contained 51.55% of CBDA. Cultivar II had the least number of cannabinoids but contains the highest amount of ∆9-THCA (94.8%) in the leaf sample. Cultivar II showed ∆9-THCA and ∆9-THC dominant profiles with less than 2% of ∆8-THC. ∆9-THC, ∆8- THC were the only neutral cannabinoids detected in Cultivar II while CBD, ∆9-THC, and ∆8- THC were found in significant amounts in Cultivar I. The compounds isolated from the stem bark of Cultivar II were elucidated as five triterpenoids: Sativalenic acid ethyl ester (E1), Phyllanthone (E2), Friedelin (E3), Sativalonone (A20) and Sativaol (A23); an alkaloid, Sativacherine (E4); and a fatty acid ester, Ethyl stearate (A21). Four of these compounds are newly described, while all the compounds are reported for the first time from the Eastern Cape Cultivar (Cherrywine) of C. sativa stem. , Thesis (PHD) -- Faculty of Natural Sciences, 2024
- Full Text:
- Date Issued: 2024-02
- Authors: Odieka, Anwuli, Endurance
- Date: 2024-02
- Subjects: Organic chemistry
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/11260/9427 , vital:73532
- Description: Cannabis sativa L. (Family Cannabaceae) has continued to attract much interest among natural product scientists in recent time because of its known medicinal values and remarkable chemical diversity. This study reports the investigation of C. sativa cultivated in the Eastern Cape Province of South Africa, with a view to obtaining its chemical fingerprint and isolating compounds from its stem bark for possible biological applications. Fresh and dried leaves, flowers, stem barks and roots of two Cannabis cultivars were obtained under permit NO. PIA-HP-EC-2022-0023. The fresh and dried plant materials were extracted for their essential oils (EOs), using hydro-distillation method on a Clevenger apparatus set at optimum extraction condition. The EOs were characterized by a gas chromatography coupled to a mass spectrometer (GC-MS). The chemical components of the EOs were elucidated based on their retention indices, as well as with reference to literature report. Other portions of the dried leaf, inflorescence and stem bark of each cultivar were extracted with methanol. The methanol extract was profiled for its cannabinoid content using liquid chromatography mass spectrometry (LCMS) based metabolomics approach, with reference to standard cannabinoids (∆9-THC, ∆9-THCA, and CBD). Lastly, the dried stem bark of Cultivar II was investigated for its compounds. The stem bark powder was subjected to sequential extraction at room temperature with n-hexane, dichloromethane, ethyl acetate and methanol. Based on the TLC analysis, the afforded n-hexane and ethyl acetate extracts were further individually fractionated on a repeated column chromatography, using silica gel and Sephadex LH20 as stationary phases. The column fractionation process led to the isolation of seven compounds, E1-E4, A20, A21, and A23. The isolated compounds were characterized by spectroscopic and spectrometric methods on nuclear magnetic resonance (NMR) spectrometer and ultra high-performance liquid chromatography hyphenated to a mass spectrometer, respectively. The results from the study showed that the EOs of the C. sativa cultivars contain majorly sesquiterpenes in their oxygenated and non-oxygenated forms. Caryophyllene (0.6421.4%), caryophyllene oxide (1.27-20.34%), -pinene (0.35-13.21%), D-limonene (0.51-10.99%), humulene (0.37-10.56%), humulene-1,2-epoxide (0.45-5.98%), pinene (0.16-5.22%), nerolidol (0.32-4.99%), -myrcene (0.31-4.23%), -selinene (0.92-2.51%), and trans--bergamotene (0.50-2.70%), were the main terpene constituents of the EOs in both cultivars. Neutral cannabinoids were also detected in both cultivars with cannabidiol found in significant amount up to 85.03% in the fresh stem oil (LSO) of Cultivar I (Lifter). LC-MS fingerprints revealed that Cultivar I had the highest accumulation of cannabinoids with CBDA having the highest amount (88.65%) in the stembark and CBD at about 23% in the leaves. The inflorescence (flower) contained 51.55% of CBDA. Cultivar II had the least number of cannabinoids but contains the highest amount of ∆9-THCA (94.8%) in the leaf sample. Cultivar II showed ∆9-THCA and ∆9-THC dominant profiles with less than 2% of ∆8-THC. ∆9-THC, ∆8- THC were the only neutral cannabinoids detected in Cultivar II while CBD, ∆9-THC, and ∆8- THC were found in significant amounts in Cultivar I. The compounds isolated from the stem bark of Cultivar II were elucidated as five triterpenoids: Sativalenic acid ethyl ester (E1), Phyllanthone (E2), Friedelin (E3), Sativalonone (A20) and Sativaol (A23); an alkaloid, Sativacherine (E4); and a fatty acid ester, Ethyl stearate (A21). Four of these compounds are newly described, while all the compounds are reported for the first time from the Eastern Cape Cultivar (Cherrywine) of C. sativa stem. , Thesis (PHD) -- Faculty of Natural Sciences, 2024
- Full Text:
- Date Issued: 2024-02
Communal land use management and socio-spatial planning in Mooiplaas, South Africa
- Eglin, Ronald Arthur https://orcid.org/0009-0001-3983-709X
- Authors: Eglin, Ronald Arthur https://orcid.org/0009-0001-3983-709X
- Date: 2024-02
- Subjects: Land use -- Planning -- South Africa , Land use--Planning -- Law and legislation -- South Africa , Land use, Rural -- South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/29684 , vital:78871
- Description: This study explores, for the first time in southern Africa, the possibility of using a pattern language as part of a more adaptive planning process where decisions to change land use are made by municipalities and communities taking into account the extent to which these decisions respond to the ever-changing context. The objective was firstly to identify what socio-spatial planning principles (presented as a patterns) the community use and would like to use to guide communal land use, secondly to evaluate how effective land use management has been in determining land use activities, and thirdly to explore how a pattern language could be used to guide land use change decisions. Using a qualitative research methodology, 67 semi-structured individual and key informant interviews as well as three focus group discussions were conducted in two villages within the Mooiplaas communal land area. The main findings indicate that the current informal procedures for managing land use change by the community’s leadership is increasingly ineffective in guiding land use decisions in ways that prioritise environmental protection and promote economic and social development. However, using visioning and needs analysis techniques, the study demonstrates that a community can develop a pattern language that reflects a comprehensive vision for their area. This pattern language can then be expressed as local spatial development principles in the municipality’s Spatial Development Framework. Additionally, as part of its Land Use Scheme regulations, the municipality can establish natural, intensive agricultural, and settlement overlay zones, overlaid on an underlining extensive agricultural base zone. Anyone wanting to use land for any purpose not provided for in these zones has to submit a land use change application and the authorising structures are required to use the local spatial development principles to help inform them as to whether to approve the application or not. The study advances a new understanding of the pattern language as an expression of a community’s vision for a particular area and demonstrates how a pattern language planning approach could work in a communal land area in South Africa within the context of existing spatial planning legislation. , Thesis (PhD) -- Faculty of Social Sciences and Humanities, 2025
- Full Text:
- Date Issued: 2024-02
- Authors: Eglin, Ronald Arthur https://orcid.org/0009-0001-3983-709X
- Date: 2024-02
- Subjects: Land use -- Planning -- South Africa , Land use--Planning -- Law and legislation -- South Africa , Land use, Rural -- South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/29684 , vital:78871
- Description: This study explores, for the first time in southern Africa, the possibility of using a pattern language as part of a more adaptive planning process where decisions to change land use are made by municipalities and communities taking into account the extent to which these decisions respond to the ever-changing context. The objective was firstly to identify what socio-spatial planning principles (presented as a patterns) the community use and would like to use to guide communal land use, secondly to evaluate how effective land use management has been in determining land use activities, and thirdly to explore how a pattern language could be used to guide land use change decisions. Using a qualitative research methodology, 67 semi-structured individual and key informant interviews as well as three focus group discussions were conducted in two villages within the Mooiplaas communal land area. The main findings indicate that the current informal procedures for managing land use change by the community’s leadership is increasingly ineffective in guiding land use decisions in ways that prioritise environmental protection and promote economic and social development. However, using visioning and needs analysis techniques, the study demonstrates that a community can develop a pattern language that reflects a comprehensive vision for their area. This pattern language can then be expressed as local spatial development principles in the municipality’s Spatial Development Framework. Additionally, as part of its Land Use Scheme regulations, the municipality can establish natural, intensive agricultural, and settlement overlay zones, overlaid on an underlining extensive agricultural base zone. Anyone wanting to use land for any purpose not provided for in these zones has to submit a land use change application and the authorising structures are required to use the local spatial development principles to help inform them as to whether to approve the application or not. The study advances a new understanding of the pattern language as an expression of a community’s vision for a particular area and demonstrates how a pattern language planning approach could work in a communal land area in South Africa within the context of existing spatial planning legislation. , Thesis (PhD) -- Faculty of Social Sciences and Humanities, 2025
- Full Text:
- Date Issued: 2024-02
Knowledge, attitudes and perceptions towards hand hygine practice amongst students at a nursing colledge, Lesotho
- Authors: Ntaote, Malehlohonolo
- Date: 2024-02
- Language: English
- Type: Masters theses , text
- Identifier: http://hdl.handle.net/11260/13000 , vital:76187
- Description: Hands are critical vectors for microorganisms that cause hospital acquired infections, hand hygiene, being done the right way, at the right time and being given the right priority at health care setting is an effective strategy to reduce hospital acquired infections and costs. Different strategies have been put in place to help improve hand hygiene compliance among health care workers, amongst them; continuous training is advised at all levels of care. This study aimed to describe the knowledge, attitudes and perceptions towards hand hygiene in nursing students in Maluti College Berea Lesotho Methods This study was a quantitative cross-sectional study; 114 questionnaires were distributed and 103 were filled and returned by available willing students in Maluti College, that was 90% response rate. Results Females were more than males at 88% (91). Age range of the respondents were 18-35 years, with a mean of 22 years, 57% (59) were between ages 20-24 years. The students were distributed in four different class levels, lowest was third years at 19% (19) and highest was the nurse midwives at 32% (33). 75% (77) had hand hygiene training in the past three years. 62% (64) had moderate knowledge, all had positive attitudes and 61% (63) had a positive perception towards hand hygiene. There was a significant (p value=0.012) association between knowledge and training. Conclusion and recommendations The study has demonstrated a significant association between knowledge and training, indicating the pivotal role of education in shaping practices related to hand hygiene among healthcare workers. These findings underscore the importance of integrating insights garnered from this study into the existing teaching curriculum for healthcare 3 professionals. Furthermore, the results provide valuable input for enhancing the World Health Organization's (WHO) hand hygiene multimodal strategy, emphasizing the need for targeted interventions that address knowledge and training gaps. Additionally, the study findings advocate for incorporation of evidence-based practices into initiatives such as the SAFE LIFE Clean YOUR HAND campaign, thereby reinforcing the importance of continuous education and training to promote effective hand hygiene practices and mitigate the spread of healthcare-associated infections. This calls for a concerted effort from policymakers, healthcare institutions, and educational stakeholders to implement strategies that prioritize knowledge dissemination and training programs to improve hand hygiene practices among healthcare workers worldwide. , Thesis (Masters) -- Faculty of Medicine and Health Sciences, 2024
- Full Text:
- Date Issued: 2024-02
- Authors: Ntaote, Malehlohonolo
- Date: 2024-02
- Language: English
- Type: Masters theses , text
- Identifier: http://hdl.handle.net/11260/13000 , vital:76187
- Description: Hands are critical vectors for microorganisms that cause hospital acquired infections, hand hygiene, being done the right way, at the right time and being given the right priority at health care setting is an effective strategy to reduce hospital acquired infections and costs. Different strategies have been put in place to help improve hand hygiene compliance among health care workers, amongst them; continuous training is advised at all levels of care. This study aimed to describe the knowledge, attitudes and perceptions towards hand hygiene in nursing students in Maluti College Berea Lesotho Methods This study was a quantitative cross-sectional study; 114 questionnaires were distributed and 103 were filled and returned by available willing students in Maluti College, that was 90% response rate. Results Females were more than males at 88% (91). Age range of the respondents were 18-35 years, with a mean of 22 years, 57% (59) were between ages 20-24 years. The students were distributed in four different class levels, lowest was third years at 19% (19) and highest was the nurse midwives at 32% (33). 75% (77) had hand hygiene training in the past three years. 62% (64) had moderate knowledge, all had positive attitudes and 61% (63) had a positive perception towards hand hygiene. There was a significant (p value=0.012) association between knowledge and training. Conclusion and recommendations The study has demonstrated a significant association between knowledge and training, indicating the pivotal role of education in shaping practices related to hand hygiene among healthcare workers. These findings underscore the importance of integrating insights garnered from this study into the existing teaching curriculum for healthcare 3 professionals. Furthermore, the results provide valuable input for enhancing the World Health Organization's (WHO) hand hygiene multimodal strategy, emphasizing the need for targeted interventions that address knowledge and training gaps. Additionally, the study findings advocate for incorporation of evidence-based practices into initiatives such as the SAFE LIFE Clean YOUR HAND campaign, thereby reinforcing the importance of continuous education and training to promote effective hand hygiene practices and mitigate the spread of healthcare-associated infections. This calls for a concerted effort from policymakers, healthcare institutions, and educational stakeholders to implement strategies that prioritize knowledge dissemination and training programs to improve hand hygiene practices among healthcare workers worldwide. , Thesis (Masters) -- Faculty of Medicine and Health Sciences, 2024
- Full Text:
- Date Issued: 2024-02
The psychosocial experiences of women and children associated with development induced internal displacement caused by the Tokwe-Mukosi Dam project, Zimbabwe
- Authors: Kufakunesu, Robert
- Date: 2024-02
- Subjects: Displacement (Psychology) , Human behavior , Displacement behavior in humans
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/29867 , vital:79028
- Description: Many development projects have resulted in the displacement of whole communities in Zimbabwe in recent decades. Most of these displacements have translated into extreme disadvantages for women and children. Previous research has largely focused on the economic, humanitarian, and human rights issues without segregating the psychosocial experiences women and children face owing to displacements. This study examined the legal frameworks, both international and municipal, that govern the internal displacement of people for development. The socio-political context in which development-based displacements take place in Zimbabwe was also examined. Viewed through the Human Security Approach and Maslow’s Hierarchy of Needs, this study also covered the insecurities and the psychosocial challenges that women and children experienced because of development induced displacements. The non-probability sampling method and the purposive sampling technique were employed in this study. The qualitative data gathered through Focus Group Discussions and Key Informant Interviews were used to explore the experiences of women and children associated with project-based displacement caused by the Tokwe-Mukosi Dam project. The findings of the study indicated that women and children experienced loss of property, land, social networks, identity, a sense of community, impoverishment, and were subjected to resettlement without adequate compensation. To the women and children, project-based development had negative psychosocial implications. Women and children were further exposed, alienated, and disenfranchised from social amenities needed for human development and growth. From these findings the study concludes that the largely colonial policy in displacement and resettlement violates human rights and further disadvantages women in their need to participate in development. There is therefore need for engendered policies in the displacement and resettlement to ensure the protection and assistance of women and children and whole communities. Social Workers and other humanitarian players need to play major roles. In advocating social amenities and facilitating just compensation and psychosocial wellbeing of the displaced women and children. Healthy relationships with host communities should be forged by Social Workers and all stakeholders to ensure smooth resettlement of the displaced. , Thesis (MSci Soc) -- Faculty of Social Sciences and Humanities, 2024
- Full Text:
- Date Issued: 2024-02
- Authors: Kufakunesu, Robert
- Date: 2024-02
- Subjects: Displacement (Psychology) , Human behavior , Displacement behavior in humans
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/29867 , vital:79028
- Description: Many development projects have resulted in the displacement of whole communities in Zimbabwe in recent decades. Most of these displacements have translated into extreme disadvantages for women and children. Previous research has largely focused on the economic, humanitarian, and human rights issues without segregating the psychosocial experiences women and children face owing to displacements. This study examined the legal frameworks, both international and municipal, that govern the internal displacement of people for development. The socio-political context in which development-based displacements take place in Zimbabwe was also examined. Viewed through the Human Security Approach and Maslow’s Hierarchy of Needs, this study also covered the insecurities and the psychosocial challenges that women and children experienced because of development induced displacements. The non-probability sampling method and the purposive sampling technique were employed in this study. The qualitative data gathered through Focus Group Discussions and Key Informant Interviews were used to explore the experiences of women and children associated with project-based displacement caused by the Tokwe-Mukosi Dam project. The findings of the study indicated that women and children experienced loss of property, land, social networks, identity, a sense of community, impoverishment, and were subjected to resettlement without adequate compensation. To the women and children, project-based development had negative psychosocial implications. Women and children were further exposed, alienated, and disenfranchised from social amenities needed for human development and growth. From these findings the study concludes that the largely colonial policy in displacement and resettlement violates human rights and further disadvantages women in their need to participate in development. There is therefore need for engendered policies in the displacement and resettlement to ensure the protection and assistance of women and children and whole communities. Social Workers and other humanitarian players need to play major roles. In advocating social amenities and facilitating just compensation and psychosocial wellbeing of the displaced women and children. Healthy relationships with host communities should be forged by Social Workers and all stakeholders to ensure smooth resettlement of the displaced. , Thesis (MSci Soc) -- Faculty of Social Sciences and Humanities, 2024
- Full Text:
- Date Issued: 2024-02
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