Home is in the wandering
- Authors: Purdon, Bradley Michael
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435547 , vital:73167
- Description: Submitted in partial fulfilment of the requirements for the degree of Master of Arts in Creative Writing , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2024
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- Date Issued: 2024-04-04
Community violence and mental health symptoms among adolescents in South Africa: a systematic review and meta-analysis
- Authors: Jameson, Stacey
- Date: 2024-04
- Subjects: Violence -- Psychological aspects , Adolescent psychology , Community psychology , Mental health services
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/63868 , vital:73621
- Description: There is a global prevalence of youth exposure to community violence. This is notably pronounced in South Africa, where children and adolescents face elevated levels of violence. Existing systematic reviews have predominately focused on international contexts, leaving a gap that requires comprehensive analysis within the South African context to understand the situation in comparison to global trends. The objective of this study was to synthesise the available literature on adolescent exposure to community violence and its impact on mental health outcomes in South Africa. The study aimed to determine the prevalence of such exposure among South African adolescents, explore associated internalising and externalising mental health symptoms, and assess the extent of this relationship. A systematic search of electronic databases was conducted, encompassing studies published between 2010 and 2022. Eighteen studies focusing on adolescents aged 10 to 19 years, investigating exposure to community violence and mental health outcomes were included. Meta-analytical procedures revealed that South African adolescents face elevated levels of both witnessing and direct victimisation of community violence, accompanied by heightened mental health symptoms, particularly in depression and posttraumatic stress disorder. Although a moderate positive correlation between community violence exposure and depression was identified, statistical significance was not achieved. Subgroup analyses, to explore gender as a potential moderating factor was conducted. This study underscores the urgent need for targeted interventions and policy measures to address the impact of community violence on adolescent mental wellbeing in South Africa. These findings contribute to the existing literature by providing an understanding of the situation in the South African context compared to global estimates, offering recommendations for future research to inform more effective public health strategies. , Thesis (MA) -- Faculty of Health Science, School of Behavioural & Lifestyle Sciences, 2024
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- Date Issued: 2024-04
Quantifying the impact of the spatio-temporal variability of land use/land cover on surface run-off generation and groundwater recharge in the luvuvhu river catchment area as a study area
- Authors: Ramuhovhi, Dakalo Ndivhuwo Stella
- Date: 2024-04
- Subjects: Remote sensing , Geographic information systems , Groundwater flow
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/64322 , vital:73675
- Description: Assessing the spatio-temporal dynamics of land use land cover (LULC) change on hydrological response is vital for catchment sustainability and developing proper management strategies. The study aimed to assess the spatiotemporal effects and implications of LULC dynamics on surface runoff in the Luvuvhu River Catchment, Limpopo Province, using the Soil and Water Assessment Tool (SWAT) model. Satellite images of Landsat 5-thematic mapper and Landsat 8 operational land imager for the years 1990 and 2021 were used to explore the characteristics of LULC for this study by adopting the maximum likelihood (ML) supervised classification method. Five LULC classes were classified in this study; namely, water, built-up area, bare surface, dense vegetation, and sparse vegetation. The classification results show good accuracy values in the range of 76% (1990) and 84% (2021) with overall kappa of 63.8% and 72.8% for 1990 and 2021, respectively. For the purpose of this study, integration of geospatial technique and SWAT model were configured to operate at a monthly time interval over a span of 34 years, specifically from 1979 to 2013 to simulate surface runoff. The SWAT simulation process was executed using a digital elevation model, soil, LULC, and weather data. The analysis of LULC for 1990 and 2021 runoff modelling, it was found that, the runoff depth increased gradually from 3249 mm to 5162.5 mm during 1990 and 2021 LULC change, respectively. The R2, ENS, PBIAS, and RSR values for the calibration and the validation were 0.81 and 0.76, and 0.72 and 0.68, 0.64 and 0.58, 0.54 and 0.63 respectively. These values indicate good correlation between the observed and simulated stream flow data Therefore, suitable and timely management measures must be taken by policy decision-makers to enable sustainable development and to protect the catchment’s natural resources in order to reduce the severity of the changes. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2024
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- Date Issued: 2024-04
Fabrication of nanocatalysts as nanozymes-based biosensors for the detection of glucose and ascorbic acid
- Authors: Chavalala, Ridge Nhlamulo
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424501 , vital:72159
- Description: Embargoed. Expected release in 2025. , Thesis (MSc) -- Faculty of Science, Chemistry, 2023
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- Date Issued: 2023-10-13
Quantifying the work demands of municipal waste workers to determine physical workload and perception
- Authors: Mamabolo, Lerato
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424267 , vital:72138
- Description: Objective: This study aimed to quantify physical and perceptual workloads through the nature of the work demands of municipal waste workers. Waste workers investigated were waste collectors, street sweepers [or public cleansers], drivers and mechanics. Methods: 154 City of Tshwane municipal waste workers volunteered to participate in the study. Observation-based tools were used to measure physical workload namely, the RAMP 1 and 2, REBA and RULA, and the hand-held dynamometer was used for pushing and pulling tasks. Subjective perceptual questionnaires were used to measure Perceptual load namely the NASA TLX and the QWIQ. Results: Differences in physical workload were evident between the occupation groups. Round bin collection was the waste collection type pertaining to the highest work-related risk of musculoskeletal development. Public cleansers and flatbed truck collectors' results displayed lower task-related risks associated in relation to the other occupations. Significant differences were found between the groups, particularly between waste collectors and drivers [had high workload outputs] in relation to public cleansers [low workload] for both perceptual workload tools. A significant difference was found in the mental and physical demands, as well as the organisational constraints and quantitative workloads. Mechanics showed no significant differences between groups but had high levels of frustration. Conclusion: A relationship between physical and perceived workload intensity is evident. development of musculoskeletal diseases and injury shall incur if measures are not reinstated. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2023
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- Date Issued: 2023-10-13
Private suplementary tutoring and grade 12 education in Geometry performance
- Authors: Mabhamba, Mfuneko
- Date: 2023-00
- Subjects: Mathematics Education
- Language: English
- Type: Masters theses , text
- Identifier: http://hdl.handle.net/11260/10448 , vital:75181
- Description: The primary intention of this study was to explore the extent to which private tutorials relate to Euclidean Geometry performance amongst Grade 12 learners from selected schools in OR Tambo Inland District. The first objective was to examine the correlation between private tutorials and Euclidean Geometry performance. The second research question was to investigate the Euclidean Geometry means scores of students who attend private tutorials as compared to the group of students who do not attend private tutorials. The study had a sample size of 347 participants from four schools. Lev Vygotsky's Socio-cultural Theory was used as a theoretical framework to underpin the study. The study adopted quantitative research methodology to answer the research questions. A questionnaire and a standardised Euclidean Geometry test were administered to collect the data from the participants. Correlational and regression analysis were performed to analyse to data. The outcomes of the study reveal that private tutorials and Euclidean Geometry performance were significantly correlated. Furthermore, private tutorials were found to be the determinant or predictor of performance in Euclidean Geometry. Secondly, students who are attending private tutorials significantly outperformed their counterparts (i.e., students who are not attending private tutorials) in Euclidean Geometry. Recommendations were made to implicate stakeholders to enhance the effective use private tutorials to boost Euclidean Geometry performance while also improvising alternative measures to students who might want to attend private tutorials, but affordability becomes a detrimental hindrance. , Thesis (Masters) -- Faculty of Education, 2023
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- Date Issued: 2023-00
Moult and energetics in three species from the Euplectes genus representing a gradient of elaborate plumage ornamentation
- Authors: Webb, Stacey Leigh
- Date: 2022-12
- Subjects: Sexual selection in animals – South Africa , Bird watching -- Africa, Southern , Plumage
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/60026 , vital:62734
- Description: Elaborate plumage ornamentation in birds has fascinated scientists since the 1800s. The evolution and persistence of elaborate and costly ornamentation seemed to be in opposition to the theory of natural selection. However, over the years research has revealed the role and functional significance of elaborate plumage ornamentation in sexual selection. Recent studies have shown that the energetic costs associated with moulting into new plumages are higher than maintenance costs, but these costs have not been quantified for species that exhibit elaborate breeding plumage ornamentation for sexual selection. In addition, for these sexually selected ornamental traits (signals) to have evolved and be maintained, there must be some aspect of individual quality that they convey to the receiver. Despite a large body of literature on sexual selection, honest signalling and ornamental plumage in birds, the physiological correlates of honest signalling during sexual selection and the messages these signals convey to the receivers are poorly understood. In this study, I investigated four aspects related to the honesty of the sexual selection signal. These were (i) seasonal variation in standardised resting metabolic rates of breeding and non-breeding birds, (ii) moult phenology, (iii) physiological parameters linked to honest signalling, and (iv) the energetic costs of production and maintenance of elaborate plumage ornamentation using three species from the Euplectes genus as model species. The three species selected, the red-collared widowbird (Euplectes ardens), fan-tailed widowbird (Euplectes axillaris) and the southern red bishop (Euplectes orix) represent a gradient of plumage elaboration and although the receivers of the sexual selection signals are known for these three species, the energetic cost of the signals and the underlying message the signals convey remain unknown. I measured seasonal variation in resting metabolic rate (RMR), body mass (Mb), thermoneutral zone (TNZ) and body temperature (Tb) over the course of two years in the three selected Euplectes species (E. ardens, E. axillaris and E. orix; chapter 2). My results showed no significant effect of season on RMR (except in E. orix females) suggesting that in these species the metabolic costs in summer (associated with breeding season) are comparable to the metabolic costs associated with thermoregulation during winter. Contrary to my prediction that metabolic costs would increase along a gradient of increasing tail length (due to the energetic costs Webb V associated with elaborate plumage ornamentation), I found no significant effect of species. The lack of a significant result indicates that once the initial investment in plumage is complete, other predictor variables play a role (in this case body mass). , Thesis (PhD) -- Faculty of Science, School of Environmental Sciences, 2022
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- Date Issued: 2022-12
Baseline assessments for foundation phase reading in isiXhosa: a case study
- Authors: Nesi, Nolubabalo Amanda
- Date: 2022-10-14
- Subjects: Test of Early Reading Ability , Xhosa language , Reading (Elementary) Ability testing , Reading (Elementary) Social aspects South Africa Makhanda , Dynamic assessment (Education)
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/406320 , vital:70258
- Description: The main objective of this study was to investigate the use of baseline assessments for reading in isiXhosa in Foundation Phase. It focused on how teachers conduct baseline assessments, the assessment tools they use, and their responsiveness to baseline assessments data in terms of curriculum planning and practice, given the lack of explicit guidelines and tools specifically developed for African languages. The lack of clarity may lead teachers to assume that the same assessment approaches and practices apply across all official languages despite their linguistic and sociolinguistic differences. Vygotsky’s socio-cultural theory of learning and Biggs’ concept on constructive alignment are used as the theoretical and conceptual framework for this study. The premise for this study was that it would be problematic to apply similar assessment approaches and use the same tools for all official South African languages. The socio-cultural approach emphasises the importance of language learning and learning generally according to the context within which language occurs. The main research sites are two Quintile 1 schools in a township in Makhanda District. These schools have been receiving external intervention which includes a baseline assessment for isiXhosa reading from Non-Governmental Organisations (NGOs). This study sought to provide some insights on whether previous assessments have informed subsequent teaching practices. The findings of the study revealed that the most prominent tool for assessing reading in isiXhosa is Early Grade Reading Assessment (EGRA) and the tools used by external literacy interventions in the two schools were adapted versions of EGRA. Additionally, the subtasks in the baseline assessment tools used were aligned to the linguistic features of isiXhosa besides the nonsense word component. Furthermore, teachers were assessing and teaching reading at lower levels than expected in the grades 2 and 3 in this study. This study recommends that teachers respond to the baseline assessment data appropriately to develop reading competencies. In addition, there is need for an alignment between the linguistic features, the curriculum and strategies of teaching reading. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2022
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- Date Issued: 2022-10-14
Drifting towards death: a South African patient safety incident through an HFE Systems lens
- Authors: Agar, Sarah Leigh
- Date: 2022-10-14
- Subjects: Patients Safety measures , Medical errors Prevention , Human engineering , Medical care South Africa
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362716 , vital:65356
- Description: Patient Safety Incidents (PSI) are a frequent occurrence within the South African public healthcare system wherein a patient is unnecessarily maimed, harmed, killed, or put through significant trauma, emotional or physical. These incidents have a significant impact on the performance of the system and the well-being of individuals involved. Often PSI are the result of multiple system failings that provide the necessary preconditions for the PSI to occur. Thus, to provide appropriate patient safety recommendations to address and aid in the prevention of future PSI it is necessary to apply a systems approach to PSI analysis. A systems approach supports a ‘bigger picture’ view of an incident which includes looking beyond the immediate causes of a PSI and taking the different levels of the healthcare system into consideration during incident analysis. Human Factors and Ergonomics (HFE) is at its core a systems discipline and has been successfully applied to multiple fields including healthcare. HFE offers multiple incident analysis tools grounded in systems theory. The Life Esidimeni incident, a PSI that resulted in the death of 144 MHCU, is the biggest PSI in recent South African history and is therefore an important potential case study for the application of HFE systems tools within the South African healthcare context (an area that is lacking in existing literature). The objectives of this research were to (i) Systematically uncover the causal factors that led to the outcome of the of the Life Esidimeni incident; (ii) Identify critical faults, and gaps within the healthcare system that led to the Life Esidimeni PSI; and (iii) Provide proactive recommendations for future prevention of PSI. To fulfil these objectives a descriptive case study research method design was adopted using a qualitative systems-based tool, AcciMap. The application of AcciMap to Life Esidimeni enabled both the sharp end and blunt end causal factors that contributed to the outcome of the incident to be identified. Importantly this provided insight into the critical faults and gaps of the South African public healthcare system. The results of the AcciMap indicated that there were four main broad systemic faults in the system. These broad areas were categorized as key themes, which include: (i) competency, (ii) safeguards, (iii) time pressures, and (iv) vertical integration. From these key themes recommendations aimed at addressing the critical faults and gaps in the system and preventing future PSI were made. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2022
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- Date Issued: 2022-10-14
Automated machine learning plankton taxonomy pipeline
- Authors: Du Toit, Ian Charles
- Date: 2022-04
- Subjects: machine learning , Plankton -- Ecology
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/58363 , vital:59003
- Description: Plankton taxonomy is considered a multi-class classification problem. The current state-of-the-art developments in machine learning and phytoplankton taxonomy, such as MorphoCluster, include using a convolutional neural network as a feature extractor and Hierarchical Density-Based Clustering for the classification of plankton and identification of outliers. These convolutional feature extraction algorithms achieved accuracies of 0.78 during the classification process. However, these feature extraction models are trained on clean datasets. They perform very well when analysing previously encountered and well-defined classes but do not perform well when tested on raw datasets expected in field deployment. Raw plankton datasets are unbalanced; whereas some classes only have one or two samples, others can have thousands. They also exhibit many inter-class similarities with significant size differences. The data can also be in the form of low-resolution, noisy images. Phytoplankton species are also highly biodiverse, meaning that there is always a higher chance of a network encountering unknown sample types. Some samples, such as the various body parts of organisms, are easily confused with the species itself. Marine experts classifying plankton tend to group ambiguous samples according to the highest order to which they are confident they belong. This system leads to a dataset containing conflicting classes and forces the feature extraction network to overfit when training. This research aims to address these spatial issues and present a feature extraction methodology built upon existing research and novel concepts. The proposed algorithm uses feature extraction methods designed around real-world sample sets and offers an alternative approach to optimizing the features extracted and supplied to the clustering algorithm. The proposed feature extraction methods achieved scores of 0.821 when tested on the same datasets as the general feature extractor. The algorithm also consists of Auxiliary SoftMax classification branches which indicate the class prediction obtained by the feature extraction models. These branches allow for autonomous labelling of the clusters formed during the HDBSCAN algorithm being performed on the extracted features. This results in a fully automated semi-supervised plankton taxonomy pipeline which achieves a classification score of 0.775 on a real-life sample set. , Thesis (MA) -- Faculty of Engineering, the Built Environment, and Technology, 2022
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- Date Issued: 2022-04
Exploring the adaptation challenges faced by novice foundation phase teachers in the Nelson Mandela Metropole
- Authors: Grimbeek, Michelle Louise
- Date: 2022-04
- Subjects: First year teachers , Teachers -- Training of – South Africa – Nelson Mandela Municipality , Education, Primary -- South Africa -- Nelson Mandela Municipality
- Language: English
- Type: Master's theses , Thesis
- Identifier: http://hdl.handle.net/10948/56034 , vital:54941
- Description: The challenges faced by novice teachers have been widely discussed in the last decade (Caspersen & Raaen, 2014; Burkman, 2012). It is believed that the first year of teaching is found to be exceedingly challenging to the novice teacher due to the complex demands of the profession, as well as a result of a lack of training and induction into the work environment (Burkman, 2012). Researchers highlight that some of the many challenges that novice teachers experience are shortcomings in curricular planning, classroom management and discipline, instructional techniques, access to materials, and dealing with parents (Burkman, 2012). These challenges, along with feelings of inadequacy due to lack of experience in the working world, often have a negative effect on novice teachers and their ability to cope, subsequently resulting in high stress levels and burnout (Caspersen & Raaen, 2014). This qualitative study incorporated an interpretivist paradigm and used both semistructured interviews and a focus group discussion to explore the adaptation challenges experienced by novice Foundation Phase teachers in the Nelson Mandela Metropole. Grossman‟s Teacher Knowledge Theory and Vygotsky‟s Social Constructivist Theory were used to frame and make meaning of the findings of this study. The findings of this study indicate that novice teachers experience a range of challenges upon entering the working world. The numerous factors influencing these challenges experienced by novices include: the work environment; prior training and education; personal knowledge, values and attitudes toward teaching; and professional and social support received within the workplace. These findings were used to formulate guidelines to help support novice teachers in their adaptation from university to the professional work environment. , Thesis (MEd) -- Faculty of Education, 2022
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- Date Issued: 2022-04
A framework to prepare an information security awareness and training programme for a provincial government department in the Eastern Cape, South Africa.
- Authors: Potelwa, Zandile
- Date: 2022-03
- Subjects: Information technology--Security measures. , Employees--Training of. , Data encryption (Computer science)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/22289 , vital:52016
- Description: Provincial government departments do not have good audit reports on the information security section. The underlying issues are human factors associated with employee interaction with Information and Communication Technology (ICT). The problem to be addressed is how a provincial government needs to focus on employees’ information security awareness so that there is a residual improvement in information security culture to realise unqualified government audits for information security. A case study approach that focused on the provincial government departments in the Eastern Cape Province was used. The primary data was collected using semi-structured interviews containing questions related to information security awareness. Microsoft Teams was used to conduct online semi-structured interviews with 12 provincial government IT staff from two identified provincial departments. The data was analysed using thematic analysis and MS Excel for coding. The findings then were used to determine the outcome of this study which is the framework for preparing an information security awareness programme. The outcome of the study was achieved by condensing the themes that emerged in both the primary and secondary data. The framework was then explained as a way of recommending the importance of preparing information security awareness and training programmes in changing information security behaviour. The derived artefact of this study is an information security awareness framework that can be utilised in a provincial government department to increase the awareness of information security amongst government employees. The contribution of this study is a framework based on the Protection Motivation Theory and the Organisational Culture, to ascertain employees’ actions in relation to information risks and threats; requirements for preparing an information security awareness program for public sector employees and to determine the requirements to be considered when building information security culture in provincial government departments. The proposed framework can then be used to establish an information security culture within the government departments, which will mitigate security risks and threats. The significance of this study as per the constructs of ISA and training show that it can challenge thinking of how ISA can be prepared for not only provincial government but also for state-owned entities or local government. , Thesis (MCom) (Information Systems) -- University of Fort Hare, 2022
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- Date Issued: 2022-03
Role of the Amathole Marine Protected Area in protecting vulnerable and threatened reef fish
- Authors: Phillips, Moraea Megan Taberer
- Date: 2021-10-29
- Subjects: Reef fishes South Africa Amathole District Municipality , Reef fishes Conservation South Africa Amathole District Municipality , Reef ecology South Africa Amathole District Municipality , Rare fishes South Africa Amathole District Municipality , Fish populations South Africa Amathole District Municipality Monitoring , Fish declines South Africa Amathole District Municipality , Underwater videography in wildlife monitoring South Africa Amathole District Municipality , Amathole Marine Protected Area , Petrus rupestris (Red Steenbras) , Polysteganus undulosus (Seventyfour seabream) , Chrysoblephus cristiceps (Daggerhead seabream)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192152 , vital:45200
- Description: The Amathole Marine Protected Area (MPA) was first established on a voluntary basis in the 1980’s by local ski-boat fishermen and comprised three small no-take areas near East London. In 2011, the areas received official recognition as the Amathole MPA and in 2019 an offshore extension was granted, increasing the area covered from 250 km2 to over 4000 km2. Though the inshore Amathole MPA has benefitted from decades longer of reprieve from fishing activity than the offshore MPA, it is limited in both its coastal and depth extent, placing in doubt its capacity to provide meaningful protection to several heavily threatened target species. Among the numerous endemic Sparids for which the Amathole region constitutes a vital portion of their distributional range are red steenbras (Petrus rupestris), seventyfour (Polysteganus undulosus), and dageraad (Chrysoblephus cristiceps). The stocks of all three species have collapsed and urgent intervention has been recommended to enable their recovery, including protection in strategically placed MPAs. The offshore Amathole MPA covers an extensive portion of prime habitat for these and other species, but no formal research has been carried out on fish assemblages in the region. As such, data to substantiate the benefits of the inshore Amathole MPA and to provide a baseline reference for the offshore MPA are lacking. This study made use of baited remote underwater stereo-video systems (stereo-BRUVS) to survey fish assemblages in the inshore Amathole MPA, adjacent inshore exploited areas, and exploited areas seaward of the inshore MPA. Sampling was carried out in 2015 and 2016, prior to the establishment of the offshore Amathole MPA. Fish assemblages from the inshore MPA were compared with those from adjacent exploited areas within the same depth range and from the offshore zone between 76 and 112 m depth. Within the inshore zone, biomass and abundance of target species were greater inside the MPA than in adjacent exploited areas, with vulnerable fisheries species showing the strongest response to protection. Offshore assemblages consisted of fewer species and fewer fish overall than those from the inshore zone but were dominated by larger individuals and those from higher trophic levels. The size of C. cristiceps was consistent across the sampled depth range but abundance of this species declined rapidly at depths of more than 60 m. Within the inshore zone, both abundance and size of C. cristiceps were greater in the MPA than in adjacent exploited areas. The size of P. rupestris was consistent across the sampled depth range and between protected and exploited areas, but this species was considerably more abundant offshore. Where P. rupestris was detected inshore it was almost exclusively in the MPA. No difference in the size or abundance of P. undulosus was found between the inshore MPA and inshore Abstract exploited areas, but both measures were found to increase with depth, with reproductive-sized adults restricted to the offshore zone. Despite its limited benefits for larger species, the inshore Amathole MPA is a critical refuge for smaller and more resident species like C. cristiceps, demonstrating the possibilities for conservation and management of endangered species in small MPAs. Baseline abundance and size measures for P. rupestris and P. undulosus recorded here indicate that the offshore extension of the Amathole MPA is ideally situated to protect spawner stock of these species and potentially contribute to the recovery of their stocks across a wider distributional range. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2021
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- Date Issued: 2021-10-29
Exploration of challenges in bringing traditional medicine into SA’s healthcare system, using medicinal plants for treatment of waterborne diarrhoeal diseases as a case study
- Authors: Keche, Priscilla
- Date: 2019
- Subjects: Traditional medicine -- South Africa , Waterborne infection -- South Africa , Diarrhea -- South Africa , Healers -- South Africa , Medical care -- Quality control , Medicinal plants -- South Africa , Diarrhea in children -- South Africa , World Health Organization
- Language: English
- Type: thesis , text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/118035 , vital:34588
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Biotechnology Innovation Centre (RUBIC), 2019.
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- Date Issued: 2019
The role of Stress Inducible Protein 1 (STI1) in the regulation of actin dynamics
- Authors: Beckley, Samantha Joy
- Date: 2015
- Subjects: Heat shock proteins , Molecular chaperones , Actin , Microfilament proteins , Cell migration , Adenosine triphosphatase , Metastasis
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/193941 , vital:45409
- Description: Stress-inducible protein 1 (STI1) otherwise known as Hop (Hsp70/Hsp90 organising protein) is a highly conserved abundant co-chaperone of the Hsp70 and Hsp90 chaperones. STI1 acts as an adapter protein, where it regulates the transfer of protein substrates from Hsp70 to Hsp90 during the assembly of a number of chaperone-client protein complexes. The role of STI1 associating independently with non-chaperone proteins has become increasingly prominent. Recent data from colocalisation and co-sedimentation analyses in our laboratory suggested a direct interaction between STI1 and the cytoskeletal protein, actin. However, there was a lack of information on the motifs which mediated this interaction, as well as the exact role of STI1 in the regulation of cytoskeletal dynamics. Two putative actin binding motifs, DAYKKK (within the TPR2A domain) and a polyproline region (after the DP1 domain), were identified in mammalian STI1. Our data from in vitro interaction studies including surface plasmon resonance and high speed co-sedimentation assays suggested that both TPR1 and TPR2AB were required for the STI1-actin interaction, and peptides corresponding to either the DAYKKK or the polyproline motif, alone or in combination, could not block the STI1-actin interaction. Full length mSTI1 was shown to have ATPase activity and when combined with actin an increase in ATPase activity was seen. Ex vivo studies using STI1 knockdown shRNA HEK293T cells and non-targeting control shRNA HEK293T cells showed a change of F-actin morphology as well as reduction in levels of actin-binding proteins profilin, cofilin and tubulin in the STI1 knockdown cells. These data extend our understanding of the role of STI1 in regulating actin dynamics and may have implications for cell migration. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2015
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- Date Issued: 2015