- Title
- Production and characterization of a bioflocculant from a consortium of bacteria belonging to the halomonas and micrococcus genera
- Creator
- Okaiyeto, Kunle (https://orcid.org/0000-0002-7211-714X)
- Subject
- Flocculents
- Subject
- Bacteria
- Date Issued
- 2013
- Date
- 2013
- Type
- Master's theses
- Type
- text
- Identifier
- http://hdl.handle.net/10353/25822
- Identifier
- vital:64489
- Description
- The physicochemical properties of two bioflocculant producing bacteria; Halomonas sp. Okoh and Micrococcus sp. Leo were investigated. The optimum culture conditions for the individual species were determined. All the growth conditions examined for the individual bacteria were similar. Glucose and ammonium sulphate as sole carbon and nitrogen sources respectively resulted in optimum production of bioflocculant. The flocculating activity of the bioflocculants was stimulated when Al3+ was used as the coagulating aid under acidic medium. The information obtained from individual strains was used to produce a bioflocculant from the consortium of the two bacteria. After purification, the bioflocculant yields from 1L fermentation broths were 1.213 g from Halomonas sp. Okoh, 0.738 g from Micrococcus sp. Leo and 3.51 g from the consortium. The chemical analyses of the purified bioflocculants showed that they were glycoproteins. The thermostability property of the bioflocculants was investigated between 50-100oC and the results revealed that they are heat-stable. Fourier transform infrared revealed the presence of hydroxyl, carboxyl and amino groups in the bioflocculant molecules. Scaning electron microscope (SEM) images showed the structure of each bioflocculant(s) and kaolin clay before and after flocculation. From the results obtained, the idea of using the two strains in consortium for bioflocculant production resulted in an improvement in terms of flocculating activity and yield. The bioflocculants appears to have promise as an alternative to chemical flocculants used in various industrial processes such as wastewater treatment and drinking water purification.
- Description
- Thesis (MA) -- Science and Agriculture, 2013
- Format
- computer
- Format
- online resource
- Format
- application/pdf
- Format
- 1 online resource (170 leaves)
- Format
- Publisher
- University of Fort Hare
- Publisher
- Faculty of Science and Agriculture
- Language
- English
- Rights
- University of Fort Hare
- Rights
- All Rights Reserved
- Rights
- Open Access
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UFH Department of Biochemistry & Microbiology
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | Kunle O..pdf | 1 MB | Adobe Acrobat PDF | View Details Download |