Analysis of the interaction of Hsp90 with the extracellular matrix protein fibronectin (FN)
- Authors: Hunter, Morgan Campbell
- Date: 2014
- Subjects: Heat shock proteins , Fibronectins , Extracellular matrix proteins , Breast -- Cancer
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4170 , http://hdl.handle.net/10962/d1020960
- Description: Mounting evidence suggests that Hsp90 is present and functionally active in the extracellular space. The biological function of extracellular Hsp90 (eHsp90) remains relatively uncharacterized compared to that of intracellular Hsp90. eHsp90 has been shown to interact with a finite number of extracellular proteins, however, despite the identification of eHsp90 interacting proteins, the function of eHsp90 in these complexes is unknown. Several reports suggest a role for eHsp90α in cell migration and invasion. Reported targets for eHsp90 stimulated cell migration include MMPs, LRP-1, tyrosine kinase receptors and possible others unidentified. Limited studies report a role for eHsp90β. Recently, Hsp90α and Hsp90β were isolated in a complex containing fibronectin (FN) on the surface of MDA-MB-231 breast cancer cells. Herein, we report direct binding of Hsp90α and Hsp90β to FN using a solid phase binding assay and surface plasmon resonance (SPR) spectroscopy. SPR spectroscopy showed that Hsp90β bound the 70 kDa amino-terminal fragment of FN (FN70), but that binding of FN to Hsp90β was not limited to FN70. Confocal microscopy showed regions of colocalization of Hsp90 with extracellular FN matrix fibrils in Hs578T breast cancer cell lines. Treatment of Hs578T breast cancer cells with novobiocin (an Hsp90 inhibitor) and an LRP-1 blocking antibody resulted in a loss of FN matrix and FN endocytosis (novobiocin treated). Addition of exogenous Hsp90β was able to recover such effect after both treatments. FN was shown to colocalize with intracellular LRP-1 in novobiocin treated Hs578T cells. Immunoprecipitation of an LRP-1 containing complex showed the presence of Hsp90 and 70 and 120+ kDa FN fragments. Treatment of Hs578T cells with novobiocin increased the level of FN120+ bound in LRP-1 immunoprecipitate. Exogenous Hsp90β decreased the level of low and high molecular weight FN fragments in a complex with LRP-1, despite the fact that higher levels of lower molecular weight FN fragments were detected in this cell lysate compared to the other treatments. We report FN as a novel interacting protein of eHsp90. Taken together, we provide evidence for a direct role of eHsp90β in FN matrix remodeling. We suggest that Hsp90 plays a direct role in FN matrix dynamics through interaction with FN and LRP-1. The identification of FN as a novel interacting protein of eHsp90 suggests a role for Hsp90 in FN matrix remodeling, which is important for a number of fundamental cellular processes including cell migration and metastasis.
- Full Text:
- Date Issued: 2014
- Authors: Hunter, Morgan Campbell
- Date: 2014
- Subjects: Heat shock proteins , Fibronectins , Extracellular matrix proteins , Breast -- Cancer
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4170 , http://hdl.handle.net/10962/d1020960
- Description: Mounting evidence suggests that Hsp90 is present and functionally active in the extracellular space. The biological function of extracellular Hsp90 (eHsp90) remains relatively uncharacterized compared to that of intracellular Hsp90. eHsp90 has been shown to interact with a finite number of extracellular proteins, however, despite the identification of eHsp90 interacting proteins, the function of eHsp90 in these complexes is unknown. Several reports suggest a role for eHsp90α in cell migration and invasion. Reported targets for eHsp90 stimulated cell migration include MMPs, LRP-1, tyrosine kinase receptors and possible others unidentified. Limited studies report a role for eHsp90β. Recently, Hsp90α and Hsp90β were isolated in a complex containing fibronectin (FN) on the surface of MDA-MB-231 breast cancer cells. Herein, we report direct binding of Hsp90α and Hsp90β to FN using a solid phase binding assay and surface plasmon resonance (SPR) spectroscopy. SPR spectroscopy showed that Hsp90β bound the 70 kDa amino-terminal fragment of FN (FN70), but that binding of FN to Hsp90β was not limited to FN70. Confocal microscopy showed regions of colocalization of Hsp90 with extracellular FN matrix fibrils in Hs578T breast cancer cell lines. Treatment of Hs578T breast cancer cells with novobiocin (an Hsp90 inhibitor) and an LRP-1 blocking antibody resulted in a loss of FN matrix and FN endocytosis (novobiocin treated). Addition of exogenous Hsp90β was able to recover such effect after both treatments. FN was shown to colocalize with intracellular LRP-1 in novobiocin treated Hs578T cells. Immunoprecipitation of an LRP-1 containing complex showed the presence of Hsp90 and 70 and 120+ kDa FN fragments. Treatment of Hs578T cells with novobiocin increased the level of FN120+ bound in LRP-1 immunoprecipitate. Exogenous Hsp90β decreased the level of low and high molecular weight FN fragments in a complex with LRP-1, despite the fact that higher levels of lower molecular weight FN fragments were detected in this cell lysate compared to the other treatments. We report FN as a novel interacting protein of eHsp90. Taken together, we provide evidence for a direct role of eHsp90β in FN matrix remodeling. We suggest that Hsp90 plays a direct role in FN matrix dynamics through interaction with FN and LRP-1. The identification of FN as a novel interacting protein of eHsp90 suggests a role for Hsp90 in FN matrix remodeling, which is important for a number of fundamental cellular processes including cell migration and metastasis.
- Full Text:
- Date Issued: 2014
Fabrication, characterization and application of phthalocyanine-magnetite hybrid nanofibers
- Authors: Modisha, Phillimon Mokanne
- Date: 2014
- Subjects: Nanofibers , Nanoparticles , Magnetite
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4500 , http://hdl.handle.net/10962/d1013223
- Description: Magnetic nanoparticles comprising magnetite (Fe3O4) were functionalized with 3-aminopropyl-triethoxysilane forming amino functionalized magnetite nanoparticles (AMNPs). The amino group allows for conjugation with zinc octacarboxyphthalocyanine (ZnOCPc) or zinc tetracarboxyphthalocyanine (ZnTCPc) via the carboxyl group to form an amide bond. A reduced aggregation of ZnTCPc is observed after conjugation with AMNPs. The thermal stability, conjugation, morphology and the sizes of the nanoparticles and their conjugates were confirmed using thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and Powder X-ray diffractometry (PXRD), respectively. The covalent linkage of AMNPs to ZnOCPc or ZnTCPc resulted in improvement in the photophysical behavior of the phthalocyanines. Improvement in the triplet quantum yield (ΦT), singlet oxygen quantum yield (ΦΔ), triplet lifetime (τT) and singlet oxygen lifetime (τΔ) of the ZnOCPc or ZnTCPc were observed, hence improving the photosensitizers efficiency. The conjugates comprising of zinc octacarboxyphthalocyanine (ZnOCPc) and AMNPs were electrospun into fibers using polyamide-6 (PA-6). This was used for the photodegradation of Orange-G and compared with ZnOCPc-AMNPs in suspension. For ZnOCPc-AMNPs in suspension, it is noteworthy that the catalyst can be easily recovered using an external magnetic field. The singlet oxygen generation increases as we increase the fiber diameter by increasing the ZnOCPc concentration. The singlet oxygen quantum yield is higher for PA-6/ZnOCPc-AMNPs nanofibers when compared to PA-6/ZnOCPc. The rate of degradation of Orange-G increased with an increase in the singlet oxygen quantum yield. Moreover, the kinetic analysis showed that the photodecomposition of Orange-G is a first-order reaction according to the Langmuir-Hinshelwood model.
- Full Text:
- Date Issued: 2014
- Authors: Modisha, Phillimon Mokanne
- Date: 2014
- Subjects: Nanofibers , Nanoparticles , Magnetite
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4500 , http://hdl.handle.net/10962/d1013223
- Description: Magnetic nanoparticles comprising magnetite (Fe3O4) were functionalized with 3-aminopropyl-triethoxysilane forming amino functionalized magnetite nanoparticles (AMNPs). The amino group allows for conjugation with zinc octacarboxyphthalocyanine (ZnOCPc) or zinc tetracarboxyphthalocyanine (ZnTCPc) via the carboxyl group to form an amide bond. A reduced aggregation of ZnTCPc is observed after conjugation with AMNPs. The thermal stability, conjugation, morphology and the sizes of the nanoparticles and their conjugates were confirmed using thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and Powder X-ray diffractometry (PXRD), respectively. The covalent linkage of AMNPs to ZnOCPc or ZnTCPc resulted in improvement in the photophysical behavior of the phthalocyanines. Improvement in the triplet quantum yield (ΦT), singlet oxygen quantum yield (ΦΔ), triplet lifetime (τT) and singlet oxygen lifetime (τΔ) of the ZnOCPc or ZnTCPc were observed, hence improving the photosensitizers efficiency. The conjugates comprising of zinc octacarboxyphthalocyanine (ZnOCPc) and AMNPs were electrospun into fibers using polyamide-6 (PA-6). This was used for the photodegradation of Orange-G and compared with ZnOCPc-AMNPs in suspension. For ZnOCPc-AMNPs in suspension, it is noteworthy that the catalyst can be easily recovered using an external magnetic field. The singlet oxygen generation increases as we increase the fiber diameter by increasing the ZnOCPc concentration. The singlet oxygen quantum yield is higher for PA-6/ZnOCPc-AMNPs nanofibers when compared to PA-6/ZnOCPc. The rate of degradation of Orange-G increased with an increase in the singlet oxygen quantum yield. Moreover, the kinetic analysis showed that the photodecomposition of Orange-G is a first-order reaction according to the Langmuir-Hinshelwood model.
- Full Text:
- Date Issued: 2014
Petrography, geochemistry and origin of atypical sedimentary-igneous contact relationships at the base of the Hotazel Formation around Middelplaats, Northern Cape Province, RSA
- Authors: Terracin, Matthew Theodore
- Date: 2014
- Subjects: Petrology -- South Africa , Geochemistry -- South Africa , Igneous rocks -- South Africa , Manganese ores -- South Africa , Manganese ores -- Geology -- South Africa , Metasomatism (Mineralogy) , Potassium
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5059 , http://hdl.handle.net/10962/d1012985
- Description: In the Middelplaats mine area of the Kalahari manganese field, two drill holes (MP53 and MP54) intersected anomalously high-grade manganese ore sitting stratigraphically just above an igneous body (likely a dike or sill). Manganese ore located within approximate 5 meters of the contact with the underlying igneous rocks has been substantially metasomatically upgraded from 25 percent manganese, to over 40 percent whilst the dominant manganese species within the ore has been altered to hausmannite. This report demonstrates the metasomatic alteration is related to devolatilization (removal and/or remobilization of H₂O, CO₂ and CaO) due to contact metamorphism caused by the underlying igneous rocks. The Middelplaats mine is situated in the southwest corner of the Kalahari manganese field where the paleo basin shallows out and ends. Within the mine area, several stratigraphic units pinch out or are truncated by the side of the basin. This pinching out of lithological formations has led to the underlying Ongeluk Formation being in contact with the much younger units of the Hotazel Formation. Therefore, geochemical investigation into the nature and source of the igneous rocks was also undertaken to see if the rocks from the two drill holes were related to one another and/or the underlying Ongeluk Formation. Results of these geochemical studies have demonstrated that the Middelplaats igneous rocks (dolerites) from the two drill holes (MP53 and MP54) share a co-genetic source region. There is also reasonable geochemical evidence that the source region of the Middelplaats igneous rocks was substantially similar to the source region of the Ongeluk Formation. This may indicate that the source region of the Ongeluk Formation was reactivated at some later stage resulting in the emplacement of doleritic dikes or sills in the Middelplaats mine area. The Middelplaats igneous rocks were also found to have undergone a slight but pervasive potassic alteration; with most of the original plagioclase feldspar showing some level of replacement by a potassium enriched feldspar. Although no source for this potassic fluid was found, the devolatilization reaction within the manganese ore appears to have released some potassium into the surrounding rocks. This additional potassium may be responsible for some localized potassic alteration.
- Full Text:
- Date Issued: 2014
- Authors: Terracin, Matthew Theodore
- Date: 2014
- Subjects: Petrology -- South Africa , Geochemistry -- South Africa , Igneous rocks -- South Africa , Manganese ores -- South Africa , Manganese ores -- Geology -- South Africa , Metasomatism (Mineralogy) , Potassium
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5059 , http://hdl.handle.net/10962/d1012985
- Description: In the Middelplaats mine area of the Kalahari manganese field, two drill holes (MP53 and MP54) intersected anomalously high-grade manganese ore sitting stratigraphically just above an igneous body (likely a dike or sill). Manganese ore located within approximate 5 meters of the contact with the underlying igneous rocks has been substantially metasomatically upgraded from 25 percent manganese, to over 40 percent whilst the dominant manganese species within the ore has been altered to hausmannite. This report demonstrates the metasomatic alteration is related to devolatilization (removal and/or remobilization of H₂O, CO₂ and CaO) due to contact metamorphism caused by the underlying igneous rocks. The Middelplaats mine is situated in the southwest corner of the Kalahari manganese field where the paleo basin shallows out and ends. Within the mine area, several stratigraphic units pinch out or are truncated by the side of the basin. This pinching out of lithological formations has led to the underlying Ongeluk Formation being in contact with the much younger units of the Hotazel Formation. Therefore, geochemical investigation into the nature and source of the igneous rocks was also undertaken to see if the rocks from the two drill holes were related to one another and/or the underlying Ongeluk Formation. Results of these geochemical studies have demonstrated that the Middelplaats igneous rocks (dolerites) from the two drill holes (MP53 and MP54) share a co-genetic source region. There is also reasonable geochemical evidence that the source region of the Middelplaats igneous rocks was substantially similar to the source region of the Ongeluk Formation. This may indicate that the source region of the Ongeluk Formation was reactivated at some later stage resulting in the emplacement of doleritic dikes or sills in the Middelplaats mine area. The Middelplaats igneous rocks were also found to have undergone a slight but pervasive potassic alteration; with most of the original plagioclase feldspar showing some level of replacement by a potassium enriched feldspar. Although no source for this potassic fluid was found, the devolatilization reaction within the manganese ore appears to have released some potassium into the surrounding rocks. This additional potassium may be responsible for some localized potassic alteration.
- Full Text:
- Date Issued: 2014
Aspects of the bioavailability of topical corticosteroid formulations
- Authors: Magnus, Ashley Denis
- Date: 2013-02-12
- Subjects: Adrenocortical hormones -- Therapeutic use , Dermatologic agents , Transdermal medication -- Evaluation , Dermatopharmacology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3843 , http://hdl.handle.net/10962/d1009516 , Adrenocortical hormones -- Therapeutic use , Dermatologic agents , Transdermal medication -- Evaluation , Dermatopharmacology
- Description: Two possible variables of the McKenzie/Stoughton blanching assay, namely amount applied to the test site and occlusion time have been investigated. Subsequently, two topical steroid preparations, Synalar cream (0,025% fluocinolone acetonide) and Betnovate cream (0,1% betamethasone 17-valer ate) were extemporaneously diluted with five and six placebo bases respectively. Taking cognizance of the two possible variables, these diluted preparations were assessed in vivo using a modified version of the McKenzie/Stoughton blanching assay for blanching activity over a 14 month period. It was found that the base E45, which is slightly alkali, had the greatest effect on both preparations. In the case of betamethasone 17-valerate this base c aused the conversion to the less active isomer, betamethasone 21-valerate whereas at the end of the 14 month test period it was found that the Synalar/E45 dilution contained no fluocinolone acetonide. Quantitative analysis of all the diluted preparations by high performance liquid chromatography using a reverse-phase system was performed. The data obtained f r om the systematic stUdies of the effects of varying concentrations and occlusion times were presented at the Eleventh National Congress of the South African Pharmacological Society. , KMBT_363 , Adobe Acrobat 9.53 Paper Capture Plug-in
- Full Text:
- Authors: Magnus, Ashley Denis
- Date: 2013-02-12
- Subjects: Adrenocortical hormones -- Therapeutic use , Dermatologic agents , Transdermal medication -- Evaluation , Dermatopharmacology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3843 , http://hdl.handle.net/10962/d1009516 , Adrenocortical hormones -- Therapeutic use , Dermatologic agents , Transdermal medication -- Evaluation , Dermatopharmacology
- Description: Two possible variables of the McKenzie/Stoughton blanching assay, namely amount applied to the test site and occlusion time have been investigated. Subsequently, two topical steroid preparations, Synalar cream (0,025% fluocinolone acetonide) and Betnovate cream (0,1% betamethasone 17-valer ate) were extemporaneously diluted with five and six placebo bases respectively. Taking cognizance of the two possible variables, these diluted preparations were assessed in vivo using a modified version of the McKenzie/Stoughton blanching assay for blanching activity over a 14 month period. It was found that the base E45, which is slightly alkali, had the greatest effect on both preparations. In the case of betamethasone 17-valerate this base c aused the conversion to the less active isomer, betamethasone 21-valerate whereas at the end of the 14 month test period it was found that the Synalar/E45 dilution contained no fluocinolone acetonide. Quantitative analysis of all the diluted preparations by high performance liquid chromatography using a reverse-phase system was performed. The data obtained f r om the systematic stUdies of the effects of varying concentrations and occlusion times were presented at the Eleventh National Congress of the South African Pharmacological Society. , KMBT_363 , Adobe Acrobat 9.53 Paper Capture Plug-in
- Full Text:
In vitro anti-HIV activities of Sutherlandia frutescens and Lobostemon trigonum extracts
- Authors: Harnett, Siobhán Margaret
- Date: 2004
- Subjects: Medicinal plants -- South Africa , Materia medica, Vegetable -- South Africa , HIV infections -- Alternative treatment -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11072 , http://hdl.handle.net/10948/347 , Medicinal plants -- South Africa , Materia medica, Vegetable -- South Africa , HIV infections -- Alternative treatment -- South Africa
- Description: Currently, the approved anti-HIV drugs on the market only target the three HIV enzymes: reverse transcriptase, protease and more recently, integrase. Due to the limited nature of the current therapy, it is possible that a multi-drug resistant virus can emerge. The main concerns in developing countries however, are the expense and availability of the drugs and because of this, it is essential to investigate all alternatives. Traditional medicine offers many advantages as compared to allopathic treatment in so far as being relatively cheaper, accessible and it is broadly accepted in the population groups of the developing countries. Little is known though, of the exact efficacy and toxicity of these remedies so it is vital that these possible leads be investigated thoroughly. For the purpose of this study, two plants, Sutherlandia frutescens and Lobostemon trigonum were studied to ascertain their potential anti-HIV activity. Sutherlandia has received international attention as a possible cheap herbal remedy to improve the health of AIDS sufferers. Anecdotal evidence from health workers claim that HIV- infected patients on Sutherlandia treatment have shown improved CD4 counts, decreased viral loads and a general improvement in well-being. Extracts were prepared from dried leaves and flowers in methanol, ethanol, acetone, methylene dichloride or distilled water. Sulphated polysaccharides have been described extensively in literature with regards to their anti-HIV activity, so as a form of dereplication; an ethanol precipitation was performed on the aqueous extracts to remove sulphated polysaccharides. A toxicity study was performed on all crude extracts using uninfected peripheral mononuclear blood cells (PBMCs) isolated from whole blood. To measure anti-HIV activity, HIV-infected PBMCs were cultured with each of the crude extracts and cell viability measured using the tetrazolium salt, XTT. HIV-infected CEM-NKR-CCR5 cells were also used and supernatant from the viral studies was tested for the HIV antigen p24. xii Results varied greatly between assays but with the inclusion of a point-scale system to evaluate the extracts it was clear that overall the organic extracts of the Sutherlandia flowers, especially the acetone extract (SFA), showed great anti-HIV potential. SFA in every case decreased p24 levels and in the toxicity study did not decrease cell proliferation. With the HIV-infected PBMCs SFA actually helped improve cell proliferation despite the infection. To determine the specific anti- HIV activity, all crude extracts were tested for inhibition of HIV-I reverse transcriptase, the glycohydrolase enzymes: a-glucosidase, ß-glucosidase, ßglucuronidase, HIV-I integrase and HIV-II protease. No significant inhibition was seen with these experiments except for the HIV-I RT assay. The aqueous extract of the Lobostemon leaves produced an inhibitor of HIV-RT with a very low IC50 value of 0.049mg/ml. Some inhibitory effect was lost with the removal of the sulphated polysaccharides and the addition of BSA to the assay, but still 64% inhibition of the HIVRT remained, which confirmed that the inhibitor could be something novel, and not of the polysaccharide or tannin compounds.
- Full Text:
- Date Issued: 2004
- Authors: Harnett, Siobhán Margaret
- Date: 2004
- Subjects: Medicinal plants -- South Africa , Materia medica, Vegetable -- South Africa , HIV infections -- Alternative treatment -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11072 , http://hdl.handle.net/10948/347 , Medicinal plants -- South Africa , Materia medica, Vegetable -- South Africa , HIV infections -- Alternative treatment -- South Africa
- Description: Currently, the approved anti-HIV drugs on the market only target the three HIV enzymes: reverse transcriptase, protease and more recently, integrase. Due to the limited nature of the current therapy, it is possible that a multi-drug resistant virus can emerge. The main concerns in developing countries however, are the expense and availability of the drugs and because of this, it is essential to investigate all alternatives. Traditional medicine offers many advantages as compared to allopathic treatment in so far as being relatively cheaper, accessible and it is broadly accepted in the population groups of the developing countries. Little is known though, of the exact efficacy and toxicity of these remedies so it is vital that these possible leads be investigated thoroughly. For the purpose of this study, two plants, Sutherlandia frutescens and Lobostemon trigonum were studied to ascertain their potential anti-HIV activity. Sutherlandia has received international attention as a possible cheap herbal remedy to improve the health of AIDS sufferers. Anecdotal evidence from health workers claim that HIV- infected patients on Sutherlandia treatment have shown improved CD4 counts, decreased viral loads and a general improvement in well-being. Extracts were prepared from dried leaves and flowers in methanol, ethanol, acetone, methylene dichloride or distilled water. Sulphated polysaccharides have been described extensively in literature with regards to their anti-HIV activity, so as a form of dereplication; an ethanol precipitation was performed on the aqueous extracts to remove sulphated polysaccharides. A toxicity study was performed on all crude extracts using uninfected peripheral mononuclear blood cells (PBMCs) isolated from whole blood. To measure anti-HIV activity, HIV-infected PBMCs were cultured with each of the crude extracts and cell viability measured using the tetrazolium salt, XTT. HIV-infected CEM-NKR-CCR5 cells were also used and supernatant from the viral studies was tested for the HIV antigen p24. xii Results varied greatly between assays but with the inclusion of a point-scale system to evaluate the extracts it was clear that overall the organic extracts of the Sutherlandia flowers, especially the acetone extract (SFA), showed great anti-HIV potential. SFA in every case decreased p24 levels and in the toxicity study did not decrease cell proliferation. With the HIV-infected PBMCs SFA actually helped improve cell proliferation despite the infection. To determine the specific anti- HIV activity, all crude extracts were tested for inhibition of HIV-I reverse transcriptase, the glycohydrolase enzymes: a-glucosidase, ß-glucosidase, ßglucuronidase, HIV-I integrase and HIV-II protease. No significant inhibition was seen with these experiments except for the HIV-I RT assay. The aqueous extract of the Lobostemon leaves produced an inhibitor of HIV-RT with a very low IC50 value of 0.049mg/ml. Some inhibitory effect was lost with the removal of the sulphated polysaccharides and the addition of BSA to the assay, but still 64% inhibition of the HIVRT remained, which confirmed that the inhibitor could be something novel, and not of the polysaccharide or tannin compounds.
- Full Text:
- Date Issued: 2004
Size-fractionated phytoplankton biomass and primary production in the Southern Ocean
- Authors: Balarin, Marianne G
- Date: 1999
- Subjects: Biomass -- Analysis , Phytoplankton , Atlantic Ocean
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5765 , http://hdl.handle.net/10962/d1005453 , Biomass -- Analysis , Phytoplankton , Atlantic Ocean
- Description: The factors controlling primary production in the Southern Ocean were investigated over two years during two cruises of the South African National Antarctic Program (SANAP). The first cruise was conducted to the region of the eastern Atlantic sector of the Southern Ocean during the collaborative Scandinavian/South African Antarctic expedition conducted in austral summer (DecemberIFebruary) 1997-1998. Production studies were conducted in the vicinity of the Marginal Ice Zone (MIZ), Interfrontal Zone (IFZ) and Antarctic Polar Front(APF). The second cruise was conducted during the Third Marion Island Oceanographic Survey (MIOS III) to the region of the Sub-Antarctic Prince Edward Islands in austral autumn (April/May) 1998. Size-fractionated production rates were estimated by 14C incorporation using standard JGOFS protocols. Oceanographic data from the first cruise suggest that the three regions can be divided into two distinct regimes. Stations occupied in the vicinity of the MIZ and the APF were characterised by a shallow mixed layer depth « 40m) while at the IFZ-stations, the mixed layer depth exceeded the 1% light depth. Microphytoplankton dominated integrated chlorophyll-a biomass in the MIZ (total chlorophyll a ranged between 15.4 and 41.3 mg Chi-a. m-2) and at the APF (range between 10.7 and 31.4 mg Chi-a. m-2) , comprising > 50% of total chlorophyll-a at all these stations. Xl Within the IFZ (2 stations), nanophytoplankton dominated total integrated Chl-a biomass (range between 5.6 and 8.8 mg Chi-a. m-2) comprising, on average, 36% of the total. Picophytoplankton comprised an average of 12% of the total Chl-a biomass (range between 3.1 and 5.9 mg Chi-a. m-2) in the MIZ, 36% in the IFZ (range between 6.4 and 7.8 mg Chl-a . m-2) and 20% in the vicinity of the APF (range between 6.8 and 10.6 mg Chi-a. m-2). Total integrated primary production ranged between 316 and 729 mg C . m-2. d-1 at stations occupied in the vicinity of the MIZ, and between 292 and 317 mg C . m·2• d-l within the IFZ. At stations occupied in the region of the APF, total integrated production ranged between 708 and 926 mg C . m-2• dol. The contribution of various size fractions to total productivity generally displayed the same pattern as integrated Chl-a biomass. Microphytoplankton formed the most important contributor to total production at stations occupied in the MIZ and at the APF. Within the IFZ, nanophytoplankton dominated total daily production. Nutrient data suggest that concentrations of macro nutrients within the upper water column were above the threshold where growth would be limited. Preliminary results showed that concentrations of iron (Fe) were highest in the southern region of the MIZ and in the vicinity of the APF. During the second cruise, conducted in the vicinity of the Sub-Antarctic Front (SAF) and in the upstream, inter-island and downstream regions of the Prince Edward Islands, there was evidence of fresh water run-off from the islands, (i.e. decreased salinities and increased concentrations of ammonia and nitrate). Oceanographic data collected at the various production stations indicated that the upper water column was well mixed throughout the survey. Total integrated biomass during the study ranged between 8.5 and 20.1 mg Chi-a. m-2• No distinct patterns in total Chl-a biomass were evident. Picophytoplankton dominated total biomass comprising> 45 % of total pigment at all stations. Nanophytoplankton were the second most important contributor to total integrated biomass. Generally xu microphytoplankton contributed < 10 % of total ChI-a. Total daily integrated production was highest (442.6 mg Chi-a. m-2) at the single station occupied in the vicinity of the SAF. Outside this region, total areal production was lower, ranging from 94.5 to 353.0 mg C . m-2. d-1. With the exception of the station occupied in the vicinity of the SAF, total productivity was dominated by nanophytoplankton, which comprised between 48 and 66% of the total. Concentrations of macronutrients did not appear to be limiting to phytoplankton growth. The absence of a phytoplankton bloom in the vicinity of the islands appears to have been related to water column stability, which was influenced by the prevailing oceanographic regime during the survey. Previous studies have shown that when the SAF lies in close proximity to the islands, advecting forces prevail, resulting in the islands functioning as a flow-through system. During this study, the SAF lay immediately north of the islands. As a consequence no water was trapped in the leeward side of the islands. The results of the two cruises suggest that phytoplankton production in the four systems investigated: the Marginal Ice Zone (MIZ), Antarctic Polar Front (APF), Inter Frontal Zone (IFZ) and Prince Edward Islands (PEl), was largely controlled by water column stability. It is probable that the availability of iron, particularly in the region of the MIZ and APF, may have further contributed to the elevated production recorded in these two regions.
- Full Text:
- Date Issued: 1999
- Authors: Balarin, Marianne G
- Date: 1999
- Subjects: Biomass -- Analysis , Phytoplankton , Atlantic Ocean
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5765 , http://hdl.handle.net/10962/d1005453 , Biomass -- Analysis , Phytoplankton , Atlantic Ocean
- Description: The factors controlling primary production in the Southern Ocean were investigated over two years during two cruises of the South African National Antarctic Program (SANAP). The first cruise was conducted to the region of the eastern Atlantic sector of the Southern Ocean during the collaborative Scandinavian/South African Antarctic expedition conducted in austral summer (DecemberIFebruary) 1997-1998. Production studies were conducted in the vicinity of the Marginal Ice Zone (MIZ), Interfrontal Zone (IFZ) and Antarctic Polar Front(APF). The second cruise was conducted during the Third Marion Island Oceanographic Survey (MIOS III) to the region of the Sub-Antarctic Prince Edward Islands in austral autumn (April/May) 1998. Size-fractionated production rates were estimated by 14C incorporation using standard JGOFS protocols. Oceanographic data from the first cruise suggest that the three regions can be divided into two distinct regimes. Stations occupied in the vicinity of the MIZ and the APF were characterised by a shallow mixed layer depth « 40m) while at the IFZ-stations, the mixed layer depth exceeded the 1% light depth. Microphytoplankton dominated integrated chlorophyll-a biomass in the MIZ (total chlorophyll a ranged between 15.4 and 41.3 mg Chi-a. m-2) and at the APF (range between 10.7 and 31.4 mg Chi-a. m-2) , comprising > 50% of total chlorophyll-a at all these stations. Xl Within the IFZ (2 stations), nanophytoplankton dominated total integrated Chl-a biomass (range between 5.6 and 8.8 mg Chi-a. m-2) comprising, on average, 36% of the total. Picophytoplankton comprised an average of 12% of the total Chl-a biomass (range between 3.1 and 5.9 mg Chi-a. m-2) in the MIZ, 36% in the IFZ (range between 6.4 and 7.8 mg Chl-a . m-2) and 20% in the vicinity of the APF (range between 6.8 and 10.6 mg Chi-a. m-2). Total integrated primary production ranged between 316 and 729 mg C . m-2. d-1 at stations occupied in the vicinity of the MIZ, and between 292 and 317 mg C . m·2• d-l within the IFZ. At stations occupied in the region of the APF, total integrated production ranged between 708 and 926 mg C . m-2• dol. The contribution of various size fractions to total productivity generally displayed the same pattern as integrated Chl-a biomass. Microphytoplankton formed the most important contributor to total production at stations occupied in the MIZ and at the APF. Within the IFZ, nanophytoplankton dominated total daily production. Nutrient data suggest that concentrations of macro nutrients within the upper water column were above the threshold where growth would be limited. Preliminary results showed that concentrations of iron (Fe) were highest in the southern region of the MIZ and in the vicinity of the APF. During the second cruise, conducted in the vicinity of the Sub-Antarctic Front (SAF) and in the upstream, inter-island and downstream regions of the Prince Edward Islands, there was evidence of fresh water run-off from the islands, (i.e. decreased salinities and increased concentrations of ammonia and nitrate). Oceanographic data collected at the various production stations indicated that the upper water column was well mixed throughout the survey. Total integrated biomass during the study ranged between 8.5 and 20.1 mg Chi-a. m-2• No distinct patterns in total Chl-a biomass were evident. Picophytoplankton dominated total biomass comprising> 45 % of total pigment at all stations. Nanophytoplankton were the second most important contributor to total integrated biomass. Generally xu microphytoplankton contributed < 10 % of total ChI-a. Total daily integrated production was highest (442.6 mg Chi-a. m-2) at the single station occupied in the vicinity of the SAF. Outside this region, total areal production was lower, ranging from 94.5 to 353.0 mg C . m-2. d-1. With the exception of the station occupied in the vicinity of the SAF, total productivity was dominated by nanophytoplankton, which comprised between 48 and 66% of the total. Concentrations of macronutrients did not appear to be limiting to phytoplankton growth. The absence of a phytoplankton bloom in the vicinity of the islands appears to have been related to water column stability, which was influenced by the prevailing oceanographic regime during the survey. Previous studies have shown that when the SAF lies in close proximity to the islands, advecting forces prevail, resulting in the islands functioning as a flow-through system. During this study, the SAF lay immediately north of the islands. As a consequence no water was trapped in the leeward side of the islands. The results of the two cruises suggest that phytoplankton production in the four systems investigated: the Marginal Ice Zone (MIZ), Antarctic Polar Front (APF), Inter Frontal Zone (IFZ) and Prince Edward Islands (PEl), was largely controlled by water column stability. It is probable that the availability of iron, particularly in the region of the MIZ and APF, may have further contributed to the elevated production recorded in these two regions.
- Full Text:
- Date Issued: 1999
Generalisations of filters and uniform spaces
- Authors: Muraleetharan, Murugiah
- Date: 1997
- Subjects: Filters (Mathematics) , Uniform spaces
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5409 , http://hdl.handle.net/10962/d1005223 , Filters (Mathematics) , Uniform spaces
- Description: The notion of a filter F ∈ 2²x has been extended to that of a : prefilter: ƒ ∈ 1²x, generalised filter ƒ ∈ 2²x x and fuzzy filter ᵩ ∈ 1¹x. A uniformity is a filter with some other conditions and the notion of a uniformity D ∈ 2²xxx has been extended to that of a : fuzzy uniformity d ∈ 1²xxx , generalised uniformity ∈ 1²xxx and super uniformity b ∈ 1¹x. We establish categorical embeddings from the category of uniform spaces into the categories of fuzzy uniform spaces, generalised uniform spaces and super uniform spaces and also categorical embeddings into the category of super uniform spaces from the categories of fuzzy uniform spaces and generalised uniform spaces.
- Full Text:
- Date Issued: 1997
- Authors: Muraleetharan, Murugiah
- Date: 1997
- Subjects: Filters (Mathematics) , Uniform spaces
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5409 , http://hdl.handle.net/10962/d1005223 , Filters (Mathematics) , Uniform spaces
- Description: The notion of a filter F ∈ 2²x has been extended to that of a : prefilter: ƒ ∈ 1²x, generalised filter ƒ ∈ 2²x x and fuzzy filter ᵩ ∈ 1¹x. A uniformity is a filter with some other conditions and the notion of a uniformity D ∈ 2²xxx has been extended to that of a : fuzzy uniformity d ∈ 1²xxx , generalised uniformity ∈ 1²xxx and super uniformity b ∈ 1¹x. We establish categorical embeddings from the category of uniform spaces into the categories of fuzzy uniform spaces, generalised uniform spaces and super uniform spaces and also categorical embeddings into the category of super uniform spaces from the categories of fuzzy uniform spaces and generalised uniform spaces.
- Full Text:
- Date Issued: 1997
Structures and thermal behaviour of some monooxalato and dioxalato metal complexes
- Authors: Bacsa, John
- Date: 1996
- Subjects: Oxalates -- Research , Crystallography -- Research , Chemistry, Inorganic -- Research
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4351 , http://hdl.handle.net/10962/d1005016 , Oxalates -- Research , Crystallography -- Research , Chemistry, Inorganic -- Research
- Description: The crystal structure of Ba [Cu(C₂0₄)₂(H₂O)].5H₂O has been determined using single crystal X-ray diffractometry. It crystallises in the triclinic system, space group Pī , with a = 6.539(2) Å, b = 9.211(3) Å, c = 10.928(3) Å, a = 85.42(3)°, β = 79.22(3)° , γ = 80.30(3)°, V = 636.08(8)ų and Z = 2. The structure consists of [Cu(C₂0₄)₂(H₂O)]²⁻ ions weakly bridged by barium ions and water molecules. The copper(II) ions are in a tetragonally elongated square-pyramidal environment with some trigonal distortion. The two oxalate groups occupy the equatorial positions and a water molecule occupies the axial position. The barium ion is surrounded by nine oxygens: five oxygens from water molecules and four oxygens from oxalate groups. The thermal behaviour of Ba [Cu(C₂0₄)₂(H₂O)].5H₂0 in N₂ has been examined using thermogravimetry (TG) and differential scanning calorimetry (DSC). The dehydration starts at relatively low temperatures (~80°C), but continues until the onset of the decomposition (~280°C). The decomposition takes place in two major stages. The mass of the intermediate after the first stage corresponded to the formation of barium oxalate and copper metal and, after the second stage, to the formation of barium carbonate and copper metal. The enthalpy for the dehydration was found to be 311 ±30 kJ mol⁻¹. The overall enthalpy change for the decomposition of Ba[Cu(C₂0₄)₂]in N₂ was estimated from the combined area of the peaks of the DSC curve as -347 kJ mol⁻¹. The kinetics of the thermal dehydration and decomposition were studied using isothermal TG. The dehydration was strongly deceleratory and the α-time curves could be described by the three-dimensional diffusion (D3) model. The values of the activation energy and the pre-exponential factor for the dehydration were 125 ±4 kJ mol⁻¹ and (1.38 ±0.08)x10¹⁵ min⁻¹, respectively. The decomposition was complex, consisting of at least two concurrent processes. The decomposition was analysed in terms of two overlapping deceleratory processes. One process was fast and could be described by the contracting-geometry model with n = 5. The other process was slow and could also be described by the contracting-geometry model , but with n = 2. The values of Eₐ and A were 206 ±23 kJ mol⁻¹ and (2.2 ±O.5)xl0¹⁹min⁻¹, respectively, for the fast process, and 259 ±37 kJ mol⁻¹ and (6.3 ±1.8)x10²³min⁻¹, respectively, for the slow process.The crystal structure of zinc oxalate dihydrate ([Zn(C₂0₄)(H₂O)₂]n) has also been determined by X-ray diffraction methods. It crystallises in the monoclinic system, space group C2/c with a = 11.786(2) Å, b = 5.397(1)Å, c = 9.712(1) Å, B = 126.19(5)°, V = 498.58(8)ų, Z = 4 and R = 0.037 for 435 absorption-corrected independent reflections and 50 parameters. The asymmetric unit consists of half the monomeric unit [Zn(C₂0₄)(H₂O)₂). The structure consists of infinite, linear chains of zinc ions bridged by oxalate groups. The geometry of the coordination polyhedron surrounding the zinc ion is octahedral, with the oxalate oxygens occupying the equatorial positions and water molecules occupying the axial positions.
- Full Text:
- Date Issued: 1996
- Authors: Bacsa, John
- Date: 1996
- Subjects: Oxalates -- Research , Crystallography -- Research , Chemistry, Inorganic -- Research
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4351 , http://hdl.handle.net/10962/d1005016 , Oxalates -- Research , Crystallography -- Research , Chemistry, Inorganic -- Research
- Description: The crystal structure of Ba [Cu(C₂0₄)₂(H₂O)].5H₂O has been determined using single crystal X-ray diffractometry. It crystallises in the triclinic system, space group Pī , with a = 6.539(2) Å, b = 9.211(3) Å, c = 10.928(3) Å, a = 85.42(3)°, β = 79.22(3)° , γ = 80.30(3)°, V = 636.08(8)ų and Z = 2. The structure consists of [Cu(C₂0₄)₂(H₂O)]²⁻ ions weakly bridged by barium ions and water molecules. The copper(II) ions are in a tetragonally elongated square-pyramidal environment with some trigonal distortion. The two oxalate groups occupy the equatorial positions and a water molecule occupies the axial position. The barium ion is surrounded by nine oxygens: five oxygens from water molecules and four oxygens from oxalate groups. The thermal behaviour of Ba [Cu(C₂0₄)₂(H₂O)].5H₂0 in N₂ has been examined using thermogravimetry (TG) and differential scanning calorimetry (DSC). The dehydration starts at relatively low temperatures (~80°C), but continues until the onset of the decomposition (~280°C). The decomposition takes place in two major stages. The mass of the intermediate after the first stage corresponded to the formation of barium oxalate and copper metal and, after the second stage, to the formation of barium carbonate and copper metal. The enthalpy for the dehydration was found to be 311 ±30 kJ mol⁻¹. The overall enthalpy change for the decomposition of Ba[Cu(C₂0₄)₂]in N₂ was estimated from the combined area of the peaks of the DSC curve as -347 kJ mol⁻¹. The kinetics of the thermal dehydration and decomposition were studied using isothermal TG. The dehydration was strongly deceleratory and the α-time curves could be described by the three-dimensional diffusion (D3) model. The values of the activation energy and the pre-exponential factor for the dehydration were 125 ±4 kJ mol⁻¹ and (1.38 ±0.08)x10¹⁵ min⁻¹, respectively. The decomposition was complex, consisting of at least two concurrent processes. The decomposition was analysed in terms of two overlapping deceleratory processes. One process was fast and could be described by the contracting-geometry model with n = 5. The other process was slow and could also be described by the contracting-geometry model , but with n = 2. The values of Eₐ and A were 206 ±23 kJ mol⁻¹ and (2.2 ±O.5)xl0¹⁹min⁻¹, respectively, for the fast process, and 259 ±37 kJ mol⁻¹ and (6.3 ±1.8)x10²³min⁻¹, respectively, for the slow process.The crystal structure of zinc oxalate dihydrate ([Zn(C₂0₄)(H₂O)₂]n) has also been determined by X-ray diffraction methods. It crystallises in the monoclinic system, space group C2/c with a = 11.786(2) Å, b = 5.397(1)Å, c = 9.712(1) Å, B = 126.19(5)°, V = 498.58(8)ų, Z = 4 and R = 0.037 for 435 absorption-corrected independent reflections and 50 parameters. The asymmetric unit consists of half the monomeric unit [Zn(C₂0₄)(H₂O)₂). The structure consists of infinite, linear chains of zinc ions bridged by oxalate groups. The geometry of the coordination polyhedron surrounding the zinc ion is octahedral, with the oxalate oxygens occupying the equatorial positions and water molecules occupying the axial positions.
- Full Text:
- Date Issued: 1996
Cogitator : a parallel, fuzzy, database-driven expert system
- Authors: Baise, Paul
- Date: 1994 , 2012-10-08
- Subjects: Expert systems (Computer science) , Artificial intelligence -- Computer programs , System design , Cogitator (Computer system)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4667 , http://hdl.handle.net/10962/d1006684 , Expert systems (Computer science) , Artificial intelligence -- Computer programs , System design , Cogitator (Computer system)
- Description: The quest to build anthropomorphic machines has led researchers to focus on knowledge and the manipulation thereof. Recently, the expert system was proposed as a solution, working well in small, well understood domains. However these initial attempts highlighted the tedious process associated with building systems to display intelligence, the most notable being the Knowledge Acquisition Bottleneck. Attempts to circumvent this problem have led researchers to propose the use of machine learning databases as a source of knowledge. Attempts to utilise databases as sources of knowledge has led to the development Database-Driven Expert Systems. Furthermore, it has been ascertained that a requisite for intelligent systems is powerful computation. In response to these problems and proposals, a new type of database-driven expert system, Cogitator is proposed. It is shown to circumvent the Knowledge Acquisition Bottleneck and posess many other advantages over both traditional expert systems and connectionist systems, whilst having non-serious disadvantages. , KMBT_223
- Full Text:
- Date Issued: 1994
- Authors: Baise, Paul
- Date: 1994 , 2012-10-08
- Subjects: Expert systems (Computer science) , Artificial intelligence -- Computer programs , System design , Cogitator (Computer system)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4667 , http://hdl.handle.net/10962/d1006684 , Expert systems (Computer science) , Artificial intelligence -- Computer programs , System design , Cogitator (Computer system)
- Description: The quest to build anthropomorphic machines has led researchers to focus on knowledge and the manipulation thereof. Recently, the expert system was proposed as a solution, working well in small, well understood domains. However these initial attempts highlighted the tedious process associated with building systems to display intelligence, the most notable being the Knowledge Acquisition Bottleneck. Attempts to circumvent this problem have led researchers to propose the use of machine learning databases as a source of knowledge. Attempts to utilise databases as sources of knowledge has led to the development Database-Driven Expert Systems. Furthermore, it has been ascertained that a requisite for intelligent systems is powerful computation. In response to these problems and proposals, a new type of database-driven expert system, Cogitator is proposed. It is shown to circumvent the Knowledge Acquisition Bottleneck and posess many other advantages over both traditional expert systems and connectionist systems, whilst having non-serious disadvantages. , KMBT_223
- Full Text:
- Date Issued: 1994
Exploratory studies of novel ligand systems
- Authors: Taylor, Steven John
- Date: 1992
- Subjects: Ligands , Coordination compounds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4315 , http://hdl.handle.net/10962/d1004973 , Ligands , Coordination compounds
- Description: A range of novel ligand systems have been developed in three distinct phases and preliminary studies have been initiated to evaluate their complexation potential. Phase I incorporated the synthesis of single strand ligand systems, which were mainly based on amino acid residues. Techniques have been developed for the attachment of these ligand systems onto, firstly, a styrene monomer, and then later onto a pseudo-styrene linking group, viz. the p-toluoyl group. The linking reactions were based on the formation of amides or esters by the reaction of an acid chloride system with an amine or alcohol. Phase II involved the synthesis of bis-chain ligand systems and their attachment onto the p-toluoyl linking group. A further linking group was also developed at this stage, viz. the xylyl group. In the preparation of phase II ligand systems, use was made of malonic ester and iminodiacetic acid derivatives. Phase III has involved the synthesis of cyclic ligand systems, with skeletons based upon the structures used in phase I and phase II and two crown ether type systems have been prepared.
- Full Text:
- Date Issued: 1992
- Authors: Taylor, Steven John
- Date: 1992
- Subjects: Ligands , Coordination compounds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4315 , http://hdl.handle.net/10962/d1004973 , Ligands , Coordination compounds
- Description: A range of novel ligand systems have been developed in three distinct phases and preliminary studies have been initiated to evaluate their complexation potential. Phase I incorporated the synthesis of single strand ligand systems, which were mainly based on amino acid residues. Techniques have been developed for the attachment of these ligand systems onto, firstly, a styrene monomer, and then later onto a pseudo-styrene linking group, viz. the p-toluoyl group. The linking reactions were based on the formation of amides or esters by the reaction of an acid chloride system with an amine or alcohol. Phase II involved the synthesis of bis-chain ligand systems and their attachment onto the p-toluoyl linking group. A further linking group was also developed at this stage, viz. the xylyl group. In the preparation of phase II ligand systems, use was made of malonic ester and iminodiacetic acid derivatives. Phase III has involved the synthesis of cyclic ligand systems, with skeletons based upon the structures used in phase I and phase II and two crown ether type systems have been prepared.
- Full Text:
- Date Issued: 1992
The effects of inbreeding and laboratory-rearing on a pyraustid moth, Mimorista pulchellalis Dyar (Lepidoptera: pyraustidae), imported for the biological control of jointed cactus in South Africa
- Authors: Wright, Margaret Dorothy
- Date: 1986
- Subjects: Pyralidae -- South Africa , Cactus -- South Africa , Moths -- South Africa , Insect pests -- Biological control -- South Africa , Inbreeding
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5615 , http://hdl.handle.net/10962/d1003123 , Pyralidae -- South Africa , Cactus -- South Africa , Moths -- South Africa , Insect pests -- Biological control -- South Africa , Inbreeding
- Description: Inbreeding was thought to be responsible for the loss in the second filial generation (F₂) of Amalafrida leithella Dyar, Cactoblastis mundelli Heinrich, Nanaia sp. Heinrich, Sigelgaita sp. Heinrich and Sigelgaita transilis Heinrich in the laboratory. This pre-empted the investigation of the effects of inbreeding on another cactophagous moth, Mimorista pulchellalis Dyar, an established biological control agent of jointed cactus in South Africa. Initially three populations were set up. A randomly-mating control (OUT 1) population, and a sibmating experimental (IN 1) population, consisted of laboratory-reared stock . A second experimental population (KR 1) comprised a small number of field-collected randomly-mating individuals which recreated the conditions under which the five abovementioned species were lost. The inbreeding depression of fifteen fitness components was assessed. The mean values of each component in each generation of treatments IN 1 and KR 1 were compared with those of OUT 1. In addition the mean values of IN 1 were regressed against the coefficients of inbreeding since inbreeding depression is linear with respect to the probability of two genes at any locus being homozygous through ancestry. The component egg viability was important because a reduction in OUT 1, IN 1 and KR 1 in the F₂ resulted from mated females producing no viable eggs. Duplicate treatments OUT 2, IN 2 and KR 2 were set up to confirm whether this was a general F₂ phenomenon. Assessment of the fitness components prevented a direct evaluation of the numbers of offspring produced. However a hypothetical estimate of population size and growth rate was made using the percentage survival calculated from life-table analysis. Although not statistically demonstrable in the component analysis, life table analysis indicated that egg viability suffered an inbreeding depression and affected population fitness. It was also evident that treatments OUT 1 and 2 were fitter than treatments IN 1 and 2 and KR 1 and 2 with respect to population fitness. Thus, inbreeding, resulting from sibmating and introduction of a small number of individuals to a laboratorYJ caused a decrease in numbers of offspring produced and population growth rate. This is important in relation to the demise of the cactophagous Lepidoptera and to future biological control programmes.
- Full Text:
- Date Issued: 1986
- Authors: Wright, Margaret Dorothy
- Date: 1986
- Subjects: Pyralidae -- South Africa , Cactus -- South Africa , Moths -- South Africa , Insect pests -- Biological control -- South Africa , Inbreeding
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5615 , http://hdl.handle.net/10962/d1003123 , Pyralidae -- South Africa , Cactus -- South Africa , Moths -- South Africa , Insect pests -- Biological control -- South Africa , Inbreeding
- Description: Inbreeding was thought to be responsible for the loss in the second filial generation (F₂) of Amalafrida leithella Dyar, Cactoblastis mundelli Heinrich, Nanaia sp. Heinrich, Sigelgaita sp. Heinrich and Sigelgaita transilis Heinrich in the laboratory. This pre-empted the investigation of the effects of inbreeding on another cactophagous moth, Mimorista pulchellalis Dyar, an established biological control agent of jointed cactus in South Africa. Initially three populations were set up. A randomly-mating control (OUT 1) population, and a sibmating experimental (IN 1) population, consisted of laboratory-reared stock . A second experimental population (KR 1) comprised a small number of field-collected randomly-mating individuals which recreated the conditions under which the five abovementioned species were lost. The inbreeding depression of fifteen fitness components was assessed. The mean values of each component in each generation of treatments IN 1 and KR 1 were compared with those of OUT 1. In addition the mean values of IN 1 were regressed against the coefficients of inbreeding since inbreeding depression is linear with respect to the probability of two genes at any locus being homozygous through ancestry. The component egg viability was important because a reduction in OUT 1, IN 1 and KR 1 in the F₂ resulted from mated females producing no viable eggs. Duplicate treatments OUT 2, IN 2 and KR 2 were set up to confirm whether this was a general F₂ phenomenon. Assessment of the fitness components prevented a direct evaluation of the numbers of offspring produced. However a hypothetical estimate of population size and growth rate was made using the percentage survival calculated from life-table analysis. Although not statistically demonstrable in the component analysis, life table analysis indicated that egg viability suffered an inbreeding depression and affected population fitness. It was also evident that treatments OUT 1 and 2 were fitter than treatments IN 1 and 2 and KR 1 and 2 with respect to population fitness. Thus, inbreeding, resulting from sibmating and introduction of a small number of individuals to a laboratorYJ caused a decrease in numbers of offspring produced and population growth rate. This is important in relation to the demise of the cactophagous Lepidoptera and to future biological control programmes.
- Full Text:
- Date Issued: 1986
Metallogenic evolution of the southern Appalachian Orogenic Belt and Mississippi Valley
- Authors: Maassen, Larry W
- Date: 1983 , 2013-04-03
- Subjects: Metallogeny -- Appalachian Region , Metallogeny -- Mississippi River Valley , Plate tectonics -- Appalachian Region , Plate tectonics -- Mississippi River Valley
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5018 , http://hdl.handle.net/10962/d1006210 , Metallogeny -- Appalachian Region , Metallogeny -- Mississippi River Valley , Plate tectonics -- Appalachian Region , Plate tectonics -- Mississippi River Valley
- Description: Plate tectonic theory provides logical explanations for the major tectonic events in the eastern US during Paleozoic time. The details of these tectonic events are becoming more apparent with the accumulation of new data, especially radiometric age dates. When plate tectonic theory is applied to specific tectonic events for which there is no substantial evidence, such as intracontinental hotspot rifting environments and Precambrian subduction zones, the proposed models may become very speculative. A misconception concerning the geology of the central US is that this region is structurally stable. However, geologists are currently paying considerable attention to the interlocking network of faults that in a general way follow the 38th parallel of latitude from west-central Virginia into Central Missouri (and may extend farther to the east and west). Most of the displacement along this zone occurred during the Precambrian, but different parts have moved during several periods of post-Precambrian time. In the basement the lineament may be a wide fracture zone that extends deep into the crust and is thus responsible for the magmatic iron deposits of the Southeast Missouri and may be either directly or indirectly responsible for the localization of the Mississippi Valley type deposits that occur sporadically along its length. Whether or not plate-tectonic processes operated during the Precambrian is open to speculation and the lineament may or may not be related to plate tectonic activity, but it is obvious that throughout time inherent zones of weakness are important in the localization of ore deposits. The occurrence of several major mineral districts at the intersections of the 38th parallel lineament with other major structural features, particularly in some uplifted areas and fault zone intersections, suggests that other similar structural uplifts and fault-zone intersections should be investigated for undiscovered new districts or extensions of known districts. Small uneconomic mineral occurrences along fault zones intersecting the lineament may merit further examination as they may be indications of undiscovered deposits at depth. The overall tectonic environment in the Appalachian region was an important control on the localization of massive sulfide, gold, titanium, and tungsten deposits. The deposits occur in clusters, either in Late Precambrian spreading centers and associated rift systems related to the breakup of proto-Pangea, or in Eocambrian and Devonian low-potassium tholeiitic volcanic and plutonic rocks associated with the volcanic island arc systems which developed during the closing of the Iapetus Ocean. Feiss and Hauck (1980) are confident that moderate sized (1-10 million ton) massive sulfide deposits are yet to be found at depth in these regions of the southern Appalachians, but large (greater than 20 million ton) massive sulfide deposits are unlikely to exist. The Mississippi Valley carbonate-hosted deposits of lead-zinc-baritefluorite, that occur to some extent throughout the Paleozoic section, and the Silurian "Clinton" iron ores owe their origin and distribution to normal sedimentary and diagenetic processes resulting from the transgressions of the epeiric seas. Others, such as the residual deposits of managnese iron, and aluminum, owe their existence to the afore mentioned processes, but must also have had subsequent exposure to the concentrating mechanism of weathering in a stable environment. The Mississippi Valley type occur primarily around paleo-basement highs and paleoshorelines; therefore, the formation of domes and arches within the continental interior during bathygenic episodes was a major factor controlling the localization of these deposits. These broad upwarps were preferential sites for reefal development and facies changes, and, during epeirogenic periods, these positive features have resulted in erosion and karsting of the the carbonate rocks by meteoric waters and have thus been prepared for mineralization. Deposits of this type are most common below a pre-Middle Ordovician unconformity and should be sought along major domes and arches, and along major lineaments. The association of Applachian type deposits with arches is indeterminate because a structure as subtle as an arch would be difficult to detect following overprinting by the deformation of the Alleghany orogeny; however, there is no reason to suspect that this type of positive feature did not play a role in their location. In conclusion, plate movements were a major control on the Paleozoic tectonic history of the eastern US and were also the primary control on the localization of the base metal, gold, tungsten, chromite, and titanium deposits of the southern Appalachians. However, important sedimentary and diagenetic deposits were localized primarily by arch, dome, and basin development during bathygenic episodes. Whether these submergent episodes are the result of plate motion or whether plate motion is indirectly related to submergent episodes, as suggested by Sloss and Speed (1974), remains a problem that needs to be investigated and debated further. , KMBT_363 , Adobe Acrobat 9.53 Paper Capture Plug-in
- Full Text:
- Date Issued: 1983
- Authors: Maassen, Larry W
- Date: 1983 , 2013-04-03
- Subjects: Metallogeny -- Appalachian Region , Metallogeny -- Mississippi River Valley , Plate tectonics -- Appalachian Region , Plate tectonics -- Mississippi River Valley
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5018 , http://hdl.handle.net/10962/d1006210 , Metallogeny -- Appalachian Region , Metallogeny -- Mississippi River Valley , Plate tectonics -- Appalachian Region , Plate tectonics -- Mississippi River Valley
- Description: Plate tectonic theory provides logical explanations for the major tectonic events in the eastern US during Paleozoic time. The details of these tectonic events are becoming more apparent with the accumulation of new data, especially radiometric age dates. When plate tectonic theory is applied to specific tectonic events for which there is no substantial evidence, such as intracontinental hotspot rifting environments and Precambrian subduction zones, the proposed models may become very speculative. A misconception concerning the geology of the central US is that this region is structurally stable. However, geologists are currently paying considerable attention to the interlocking network of faults that in a general way follow the 38th parallel of latitude from west-central Virginia into Central Missouri (and may extend farther to the east and west). Most of the displacement along this zone occurred during the Precambrian, but different parts have moved during several periods of post-Precambrian time. In the basement the lineament may be a wide fracture zone that extends deep into the crust and is thus responsible for the magmatic iron deposits of the Southeast Missouri and may be either directly or indirectly responsible for the localization of the Mississippi Valley type deposits that occur sporadically along its length. Whether or not plate-tectonic processes operated during the Precambrian is open to speculation and the lineament may or may not be related to plate tectonic activity, but it is obvious that throughout time inherent zones of weakness are important in the localization of ore deposits. The occurrence of several major mineral districts at the intersections of the 38th parallel lineament with other major structural features, particularly in some uplifted areas and fault zone intersections, suggests that other similar structural uplifts and fault-zone intersections should be investigated for undiscovered new districts or extensions of known districts. Small uneconomic mineral occurrences along fault zones intersecting the lineament may merit further examination as they may be indications of undiscovered deposits at depth. The overall tectonic environment in the Appalachian region was an important control on the localization of massive sulfide, gold, titanium, and tungsten deposits. The deposits occur in clusters, either in Late Precambrian spreading centers and associated rift systems related to the breakup of proto-Pangea, or in Eocambrian and Devonian low-potassium tholeiitic volcanic and plutonic rocks associated with the volcanic island arc systems which developed during the closing of the Iapetus Ocean. Feiss and Hauck (1980) are confident that moderate sized (1-10 million ton) massive sulfide deposits are yet to be found at depth in these regions of the southern Appalachians, but large (greater than 20 million ton) massive sulfide deposits are unlikely to exist. The Mississippi Valley carbonate-hosted deposits of lead-zinc-baritefluorite, that occur to some extent throughout the Paleozoic section, and the Silurian "Clinton" iron ores owe their origin and distribution to normal sedimentary and diagenetic processes resulting from the transgressions of the epeiric seas. Others, such as the residual deposits of managnese iron, and aluminum, owe their existence to the afore mentioned processes, but must also have had subsequent exposure to the concentrating mechanism of weathering in a stable environment. The Mississippi Valley type occur primarily around paleo-basement highs and paleoshorelines; therefore, the formation of domes and arches within the continental interior during bathygenic episodes was a major factor controlling the localization of these deposits. These broad upwarps were preferential sites for reefal development and facies changes, and, during epeirogenic periods, these positive features have resulted in erosion and karsting of the the carbonate rocks by meteoric waters and have thus been prepared for mineralization. Deposits of this type are most common below a pre-Middle Ordovician unconformity and should be sought along major domes and arches, and along major lineaments. The association of Applachian type deposits with arches is indeterminate because a structure as subtle as an arch would be difficult to detect following overprinting by the deformation of the Alleghany orogeny; however, there is no reason to suspect that this type of positive feature did not play a role in their location. In conclusion, plate movements were a major control on the Paleozoic tectonic history of the eastern US and were also the primary control on the localization of the base metal, gold, tungsten, chromite, and titanium deposits of the southern Appalachians. However, important sedimentary and diagenetic deposits were localized primarily by arch, dome, and basin development during bathygenic episodes. Whether these submergent episodes are the result of plate motion or whether plate motion is indirectly related to submergent episodes, as suggested by Sloss and Speed (1974), remains a problem that needs to be investigated and debated further. , KMBT_363 , Adobe Acrobat 9.53 Paper Capture Plug-in
- Full Text:
- Date Issued: 1983
A study of the interactions between phenytoin and pharmaceutical antacids, excipients and adsorbents
- Authors: Gilbert, Peter John
- Date: 1981 , 2013-03-14
- Subjects: Phenytoin , Antacids
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3821 , http://hdl.handle.net/10962/d1004935 , Phenytoin , Antacids
- Full Text:
- Date Issued: 1981
A study of the interactions between phenytoin and pharmaceutical antacids, excipients and adsorbents
- Authors: Gilbert, Peter John
- Date: 1981 , 2013-03-14
- Subjects: Phenytoin , Antacids
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3821 , http://hdl.handle.net/10962/d1004935 , Phenytoin , Antacids
- Full Text:
- Date Issued: 1981
A review of unconformity-type uranium deposits
- Authors: Dabrowski, F A
- Date: 1980 , 2013-04-02
- Subjects: Uranium ores , Mineralogy , Geochemistry , Unconformities (Geology)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4917 , http://hdl.handle.net/10962/d1003289 , Uranium ores , Mineralogy , Geochemistry , Unconformities (Geology)
- Description: Intense interest in uranium in the past decade has led to the discovery of new kinds of deposits of which the so-called unconformity-type are economically the most important. Presently known occurrences are restricted to Australia and Canada where they are characterized chiefly by their spatial relationship to Lower-Middle Proterozoic unconformities. Other common features include similar host-rock assemblages, structural controls, alteration, mineralogy, age relationships and fluid-inclusion data. Similar characteristics in other vein-type deposits, including those of the Beaverlodge district in Canada, deposits in France and Portugal, and the Schwartzwalder mine in the United States, suggest that they may also be of the unconformity-type. Various interpretations of the geological relationships of unconformity-type deposits have resulted in a number of genetic hypotheses, which require different exploration philosophies. Nearsurface supergene processes are considered to be most important although other mechanisms may have played contributing roles in the concentration of uranium. There is considerable potential for further discoveries of unconformity-type uranium deposits throughout the world. No such deposits are yet known in southern Africa although several favourable Precambrian unconformities are present.
- Full Text:
- Date Issued: 1980
- Authors: Dabrowski, F A
- Date: 1980 , 2013-04-02
- Subjects: Uranium ores , Mineralogy , Geochemistry , Unconformities (Geology)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4917 , http://hdl.handle.net/10962/d1003289 , Uranium ores , Mineralogy , Geochemistry , Unconformities (Geology)
- Description: Intense interest in uranium in the past decade has led to the discovery of new kinds of deposits of which the so-called unconformity-type are economically the most important. Presently known occurrences are restricted to Australia and Canada where they are characterized chiefly by their spatial relationship to Lower-Middle Proterozoic unconformities. Other common features include similar host-rock assemblages, structural controls, alteration, mineralogy, age relationships and fluid-inclusion data. Similar characteristics in other vein-type deposits, including those of the Beaverlodge district in Canada, deposits in France and Portugal, and the Schwartzwalder mine in the United States, suggest that they may also be of the unconformity-type. Various interpretations of the geological relationships of unconformity-type deposits have resulted in a number of genetic hypotheses, which require different exploration philosophies. Nearsurface supergene processes are considered to be most important although other mechanisms may have played contributing roles in the concentration of uranium. There is considerable potential for further discoveries of unconformity-type uranium deposits throughout the world. No such deposits are yet known in southern Africa although several favourable Precambrian unconformities are present.
- Full Text:
- Date Issued: 1980
Interpretation of mineralogical, chemical and morphological features of duricrusts and its application to mineral exploration
- Authors: Friggens, Peter John
- Date: 1980 , 2013-04-02
- Subjects: Duricrusts , Mineralogy
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5021 , http://hdl.handle.net/10962/d1006345 , Duricrusts , Mineralogy
- Description: Introduction: The object of mineral exploration is to locate economic deposits with minimum expenditure in time and money . In order to achieve this goal it is essential to select the most favourable environment and to design the search procedures for the environment in question. Duricrusts are a striking feature of tropical and subtropical landscapes where they occur as extensive, resistant horizons which form a surface or near-surface caprock and are best seen in incised landscapes. Since they cover many potentially rich mineral , provinces of the world, it is important to question what effect they have on mineral exploration. Duricrusts are defined as specific soil types formed by near-surface processes associated with weathering . In spite of the sheer aerial extent of soils over the landsurfaces of the earth, Levinson (1974) expressed surprise at how little exploration geologists know about this surficial environment . Weathering and pedogenetic processes therefore, require discussion in this text. However, in order to adhere to the scope of this dissertation only a brief description of the factors and characteristics of soil formation are given in the first chapter. In the second part, duricrusts are differentiated on the basis of composition into laterite, silcrete and calcrete. Beginning with a concise description of both natural and experimental investigations concerned with mineral decomposition and neomineralisation during weathering, the morphological, physical and chemical characters of each type are described. Environmental factors are then discussed in an attempt to identify specific duricrust types in terms of climate, geographic location, age and formation process . Once duricrust structure and texture are related to topography, specific types may be used to assess the significance of suspected markers in the landscape and permit identification of individual weathering surfaces on which mineralisation may give rise to gossans of widely different physical and chemical characteristics. The interpretation of photogeological, geochemical and geophysical data acquired during mineral exploration in duricrust terrains is problematical and some of the limitations to these search procedures are discussed in the final .chapter
- Full Text:
- Date Issued: 1980
- Authors: Friggens, Peter John
- Date: 1980 , 2013-04-02
- Subjects: Duricrusts , Mineralogy
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5021 , http://hdl.handle.net/10962/d1006345 , Duricrusts , Mineralogy
- Description: Introduction: The object of mineral exploration is to locate economic deposits with minimum expenditure in time and money . In order to achieve this goal it is essential to select the most favourable environment and to design the search procedures for the environment in question. Duricrusts are a striking feature of tropical and subtropical landscapes where they occur as extensive, resistant horizons which form a surface or near-surface caprock and are best seen in incised landscapes. Since they cover many potentially rich mineral , provinces of the world, it is important to question what effect they have on mineral exploration. Duricrusts are defined as specific soil types formed by near-surface processes associated with weathering . In spite of the sheer aerial extent of soils over the landsurfaces of the earth, Levinson (1974) expressed surprise at how little exploration geologists know about this surficial environment . Weathering and pedogenetic processes therefore, require discussion in this text. However, in order to adhere to the scope of this dissertation only a brief description of the factors and characteristics of soil formation are given in the first chapter. In the second part, duricrusts are differentiated on the basis of composition into laterite, silcrete and calcrete. Beginning with a concise description of both natural and experimental investigations concerned with mineral decomposition and neomineralisation during weathering, the morphological, physical and chemical characters of each type are described. Environmental factors are then discussed in an attempt to identify specific duricrust types in terms of climate, geographic location, age and formation process . Once duricrust structure and texture are related to topography, specific types may be used to assess the significance of suspected markers in the landscape and permit identification of individual weathering surfaces on which mineralisation may give rise to gossans of widely different physical and chemical characteristics. The interpretation of photogeological, geochemical and geophysical data acquired during mineral exploration in duricrust terrains is problematical and some of the limitations to these search procedures are discussed in the final .chapter
- Full Text:
- Date Issued: 1980
Sedimentology of the Katberg sandstone in the Eastern Cape Province
- Authors: Stavrakis, Nicholas
- Date: 1979 , 2013-10-16
- Subjects: Sedimentology -- South Africa -- Eastern Cape , Geology, Stratigraphic -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5034 , http://hdl.handle.net/10962/d1007355 , Sedimentology -- South Africa -- Eastern Cape , Geology, Stratigraphic -- South Africa -- Eastern Cape
- Description: The Katberg Sandstone is an arenaceous formation occuring stratigraphically three querters of the way up in the Beaufort Group in the south-eastern part of the main Karoo basin. A sedimentological study was carried out on the Katberg Sandstone and adjacent formations, in a 2 000 km² area south of Queenstown. Stratigraphic sections were measured and lithofacies based on the style of Miall (1977) were established for the Katberg Sandstone, Balfour and Burgersdorp Formations. Stratigraphic relationships show that the Burgersdorp Formation in the study area, is the lateral (distal) equivalent of the Katberg Sandstone. The changeover from sandstone to predominantly shale lithology occurs roughly east-west between Cathcart and Whittlesea. Stream hydraulic theory is discussed and the sedimentary structures of the various lithofacies are explained in terms of this theory. An analysis was done of thirty-four palaeochannels, and their various ancient flow characteristics were derived via multiple regression equations and a FORTRAN computer program. A statistical analysis of bedform structures, showed that flat-bedding of the upper flow regime is the dominant sedimentary structure of the Katberg Sandstone. Trough cross-bedding originating in aqueous dunes and planar cross-bedding of transverse bars, are fairly well represented while ripples are virtually absent. The opposite applies to the Balfour Formation where lower flow regime structures, viz., ripple cross-lamination and trough cross-bedding predominate. There is an even spread of (upper) flat-bedding, trough cross-bedding and ripple cross-lamination in the Burgersdorp Formation. Detailed measurement of typical short sequences from sections of the three formations, and transition matrix analyses, showed the facies assemblages to be of three stream types: (i) Katberg type: similar to Platte type of Miall (1977). Sediments were deposited by swiftly flowing, low sinuosity, braided, ephemeral streams with a predominantly sand bedload. Each flow started with preconsolidated silt and mud (flakes), and movement of exogenetic pebble lags. Channel avulsion and deposition of mud drapes in higher topographic levels of the stream were common phenomena. (ii) Balfour type: characterised by lateral accretion point bar type sequences of high sinuosity streams, and vertical accretion floodplain deposits. Lower flow regime dune and ripple bedform structures overlying (upper) flat-beds indicate a loss of stream power with channel migration. (iii) Burgersdorp type: similar to Katberg type for sandstone units, also crevasse-splays onto thick subaerially exposed floodplain aggradation reddish shales. Conventional microscopy and SEM studies showed tremendous diagenetic effects mostly in the form of secondary quartz growth in the sandstones of the three formations. Electron microprobe analyses were done on titanomagnetite grains from heavy mineral layers of the Katberg Sandstone, so that thermometric predictions could be made on provenance rocks. Palaeocurrents of the Katberg Sandstone are mostly Rank 4 and 5 types (Miall, 1974), i.e., they were generated within bars with not much change in stream orientation. Burgersdorp and Balfour formation palaeocurrents are Rank 1 - 3 types. In the case of the former this is owing to directional changes in channels as a result of frequent crevasse-splays, and in the case of the latter owing to differences in orientation between entire tracts of highly sinuous palaeostreams. Palaeocurrent directions in the Katberg Sandstone indicate a southeasterly provenance which accords with the findings of other Beaufort Group workers. Bed relief index calculations (Smith, 1970), made on Katberg rocks, considered together with the abundance of planar cross-bedding (of transverse bars) and apparent absence of longitudinal bars, testify to the deposition of the Katberg Sandstone in the distal parts of the braided stream environment. The Beaufort sediments of the study area have the sedimentological characteristics of a molasse sequence, but a different implied tectonogenesis. During Balfour sedimentation the equilibrium base was being raised very slowly at the source and complete fluvial sequences were deposited under a humid temperate climate, on a slowly subsiding plain. During Katberg/Burgersdorp times, source area tectonism was pronounced but interrupted and incomplete sequences were deposited under an arid climate. Provenance rocks for the formations studied included biotite mesoperthitic granite, granulite, charnockite, granite porphyry, granophyre, metaquartzite, greenschist and possibly diorite, epidosit e and alkaline volcanic rocks. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 1979
- Authors: Stavrakis, Nicholas
- Date: 1979 , 2013-10-16
- Subjects: Sedimentology -- South Africa -- Eastern Cape , Geology, Stratigraphic -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5034 , http://hdl.handle.net/10962/d1007355 , Sedimentology -- South Africa -- Eastern Cape , Geology, Stratigraphic -- South Africa -- Eastern Cape
- Description: The Katberg Sandstone is an arenaceous formation occuring stratigraphically three querters of the way up in the Beaufort Group in the south-eastern part of the main Karoo basin. A sedimentological study was carried out on the Katberg Sandstone and adjacent formations, in a 2 000 km² area south of Queenstown. Stratigraphic sections were measured and lithofacies based on the style of Miall (1977) were established for the Katberg Sandstone, Balfour and Burgersdorp Formations. Stratigraphic relationships show that the Burgersdorp Formation in the study area, is the lateral (distal) equivalent of the Katberg Sandstone. The changeover from sandstone to predominantly shale lithology occurs roughly east-west between Cathcart and Whittlesea. Stream hydraulic theory is discussed and the sedimentary structures of the various lithofacies are explained in terms of this theory. An analysis was done of thirty-four palaeochannels, and their various ancient flow characteristics were derived via multiple regression equations and a FORTRAN computer program. A statistical analysis of bedform structures, showed that flat-bedding of the upper flow regime is the dominant sedimentary structure of the Katberg Sandstone. Trough cross-bedding originating in aqueous dunes and planar cross-bedding of transverse bars, are fairly well represented while ripples are virtually absent. The opposite applies to the Balfour Formation where lower flow regime structures, viz., ripple cross-lamination and trough cross-bedding predominate. There is an even spread of (upper) flat-bedding, trough cross-bedding and ripple cross-lamination in the Burgersdorp Formation. Detailed measurement of typical short sequences from sections of the three formations, and transition matrix analyses, showed the facies assemblages to be of three stream types: (i) Katberg type: similar to Platte type of Miall (1977). Sediments were deposited by swiftly flowing, low sinuosity, braided, ephemeral streams with a predominantly sand bedload. Each flow started with preconsolidated silt and mud (flakes), and movement of exogenetic pebble lags. Channel avulsion and deposition of mud drapes in higher topographic levels of the stream were common phenomena. (ii) Balfour type: characterised by lateral accretion point bar type sequences of high sinuosity streams, and vertical accretion floodplain deposits. Lower flow regime dune and ripple bedform structures overlying (upper) flat-beds indicate a loss of stream power with channel migration. (iii) Burgersdorp type: similar to Katberg type for sandstone units, also crevasse-splays onto thick subaerially exposed floodplain aggradation reddish shales. Conventional microscopy and SEM studies showed tremendous diagenetic effects mostly in the form of secondary quartz growth in the sandstones of the three formations. Electron microprobe analyses were done on titanomagnetite grains from heavy mineral layers of the Katberg Sandstone, so that thermometric predictions could be made on provenance rocks. Palaeocurrents of the Katberg Sandstone are mostly Rank 4 and 5 types (Miall, 1974), i.e., they were generated within bars with not much change in stream orientation. Burgersdorp and Balfour formation palaeocurrents are Rank 1 - 3 types. In the case of the former this is owing to directional changes in channels as a result of frequent crevasse-splays, and in the case of the latter owing to differences in orientation between entire tracts of highly sinuous palaeostreams. Palaeocurrent directions in the Katberg Sandstone indicate a southeasterly provenance which accords with the findings of other Beaufort Group workers. Bed relief index calculations (Smith, 1970), made on Katberg rocks, considered together with the abundance of planar cross-bedding (of transverse bars) and apparent absence of longitudinal bars, testify to the deposition of the Katberg Sandstone in the distal parts of the braided stream environment. The Beaufort sediments of the study area have the sedimentological characteristics of a molasse sequence, but a different implied tectonogenesis. During Balfour sedimentation the equilibrium base was being raised very slowly at the source and complete fluvial sequences were deposited under a humid temperate climate, on a slowly subsiding plain. During Katberg/Burgersdorp times, source area tectonism was pronounced but interrupted and incomplete sequences were deposited under an arid climate. Provenance rocks for the formations studied included biotite mesoperthitic granite, granulite, charnockite, granite porphyry, granophyre, metaquartzite, greenschist and possibly diorite, epidosit e and alkaline volcanic rocks. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 1979
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