An evaluation of medicinal plants used in South Africa and Lesotho for uterotonic contractile purposes
- Authors: Smit, Inge
- Date: 2016
- Subjects: Traditional medicine -- South Africa Traditional medicine -- Lesotho
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/15232 , vital:40256
- Description: Background One of the leading cause of maternal mortality globally is postpartum haemorrhage (PPH), which mainly occur in developing countries. By identifying traditional medicinal plants that is used by Traditional birth attendants we can analysis the plants for cyclotides which contain uterotonic properties and a wide variety of other properties. The indigenous knowledge of traditional medicinal plants need to be preserved and scientifically proven to ensure future drug development and continued use of these plants. Cyclotides can be used as a backbone to develop novel drugs in that cyclotides are very stable proteins. Methods: Ten plants were identified that are commonly used to prevent or treat postpartum haemorrhage. The plant samples were collected, prepared and dried in South Africa. The plants were analysed in Austria. The plants underwent extraction of one gram of each plant was analysed using: Matrix-Assisted Laser desorption Ionization time-of-flight Mass Spectrometry (MALDI-TOF-MS); Tandem Mass Spectrometry and Analysis by High-performance Liquid Chromatography (HPLC). Results None of the ten plants analysed showed the presence of cyclotides. It is important to report negative results, because it informs the academic fraternity and could be entered in a global data base of results. The plants analysed is known to be used by Traditional Birth Attendants for the treatment or prevention of postpartum haemorrhage. Conclusion All the plants analysed are commonly used to prevent and treat PPH. The fact that no cyclotides were found do not dispute the practical use of these plants to prevent and treat PPH. There continue to be a gap in what uterotonic properties these plants contain and should be further investigated.
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- Date Issued: 2016
Being civil
- Authors: Matshoba, Zongezile Theophilus
- Date: 2016
- Language: English , Xhosa
- Type: Thesis , Masters , MA
- Identifier: vital:6017 , http://hdl.handle.net/10962/d1021237
- Description: My collection of short stories delves into government and governance, democracy, citizenship, civil servants, poverty, corruption and nepotism. My stories draw on the traditions of gritty urban crime fiction uncovering crimes of violence, service delivery, vandalism and corruption. They explore themes of mental cruelty and greed, self- preservation and community in rural areas, farms, townships and cities characterized by wrenching contradictions and inequalities. , This epic dramatic poetic verse delves into government and school governance, labour unions, liberation struggle, parenting and a wide range of school perceptions. It interrogates the roles of parents, teachers, students, department of education officials and that of other stakeholders that make use of schools. Influenced by William Wellington Gqoba’s ‘A great debate on education: a Parable’ wayback, it continues the education debate in the current post-democratic South Africa characterized by wrenching contradictions and inequalities. , This thesis is presented in two parts: English and isiXhosa.
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- Date Issued: 2016
Grade 12 learners' perceptions of the effect of urban agriculture on life satisfaction in Duncan Village
- Authors: Beni, Ntombomzi Octavia
- Date: 2016
- Subjects: Urban agriculture -- South Africa -- Eastern Cape Agricultural education
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10353/17861 , vital:41461
- Description: The importance of agricultural sciences in the school curriculum all over the world cannot be over-emphasized. It includes an alternative source of fresh produce, improved life satisfaction and a way to preserve cultural identity and traditions. This research study seeks to investigate the perception of Grade 12 learners on the effects of urban agriculture on life satisfaction in Duncan Village. The study adopts a descriptive survey design to elicit information from the respondents on their perceptions on the effects of urban agriculture on life satisfaction. A validated structured questionnaire whose reliability co-efficient is 0.81 was used to collect information. The simple random sampling technique was used to select 78 (seventy-eight) Grade 12 learners. The study reveals among others some components of life satisfaction as a result of urban agriculture. These components are; nutrition, self-employment, food security and poverty eradication and they have positive effects on life satisfaction of the respondents. The study recommends that the South Africa Government should make agriculture education as a compulsory subject for grade 10 and 11 learners in rural areas. This will enable them to develop entrepreneurial skills and self-reliance mentality before they finish their secondary school careers. Similarly, the practical components of this subject should constitute 50percent if not more from the overall marks. Realisation of this idea will alleviate, to some extent burden of unemployment and food scarcity as school leavers will be able to implement right away agricultural skills already acquired.
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- Date Issued: 2016
The influence of physical service recovery and online service recovery on trust and relationship retention
- Authors: Dube, Langelihle
- Date: 2016
- Subjects: Consumer complaints Customer loyalty Customer services
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10353/12766 , vital:39359
- Description: Service mishaps remain a profound topic in business due to their inevitability and detrimental impacts they bring about. As a result of this incessant detrimental effect, service recovery has been initiated into business exchanges to curb such. Moreover, service recovery programmes have been embarked on both in the offline and online delivery systems to retain this diverse need market consisting of the technological averse and experts in trading. This study therefore has to pinpoint the difficulties experienced in offline and online service recovery procedures and demarcate which of the two is the preferred channel. Service failures cut across all sectors and industries, and banking has not been spared in turn. Clients tend to choose a service recovery method based on various factors such as the panel of occurrence, technological skills and awareness, personal behaviours and available options provided by the service provider. Clients tend to choose a service recovery method based on various factors such as the panel of occurrence, technological skills and awareness, personal behaviours and available options provided by the service provider (Buttle, (2009); Clark & Melancon, (2013). The panel of occurrence depicts the method of service delivery that resulted in failed services, thus, an online service delivery is likely to attract an online resolution. Customers who transact online are highly likely to choose the same recovery method due to the associated innate benefits. Technological skills and awareness deal with the client’s articulateness in navigating the business’ website in effort to resolve the encountered problem. Personal behaviours explain that introvert clients would prefer to interact with the system and assistants online to resolve the issue while extroverts will choose the offline methods so as to experience facial interactions. Organisations sometimes detect the panel of solution based on the severity of the problem. Thus, for example, serious problems to be handled using physical means. Assessments of the degree of impact on retaining relations and gaining trust that presently employed recovery strategies pose were unearthed in this study. For physical service recovery, contact, empathy and politeness were assessed on the significance they have in recouping failed services. Responsiveness and the state of the bank’s websites will also depict the degree to which failed clients can be restored in online service delivery. Physical service recovery received greater apprehension by clients during a service error with empathy and politeness emerging as the most customer required successful strategy to enhance relations and trust thereafter. Despite its less preference, online service recovery strategies such as responsiveness and website interface resulted in significant correlations affirming their importance during service delivery and recovery. The measurement model fit quite well with sound goodness of fit indices results as per the comparison with the recommended thresholds. Moreover, the Structural Equation Model fit well with data collected.
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- Date Issued: 2016
Understanding sexual concurrency and HIV/AIDS: implicit and explicit attitudes in a South African student population
- Authors: Malataliana, Maleeto Lucy
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/3335 , vital:20484
- Description: There are more people infected with HIV in South Africa, than in any other country in the world. Studies indicate a plausible relationship between concurrently organised sexual partnership and the spread of STIs, with concurrency being accountable for as much as 74% of HIV infections in South Africa. Understanding sexual concurrency is therefore of vital importance, especially in the South African perspective. It has, however, become increasingly unreliable to rely solely on explicit self-measures to study sexual concurrency, and research has suggested that implicit cognition is a reliable alternative to understanding sexual behaviour and attitudes towards sexuality, which cannot be directly measured by explicit means. The purpose of this study was to understand sexual concurrency among a population of university students by researching their implicit and explicit attitudes towards sexual concurrency; and thereby to aid in understanding sexual concurrency in relation to the spread of HIV. A quantitative research methodology was used to analyse results from explicit measures of sexual concurrency in the form of a questionnaire, and implicit measures of sexual concurrency in the form of the Implicit Association Test (IAT). Although no correlation existed between implicit and explicit measures attitudes towards sexual concurrency, it was, however, observed that sexual concurrency has and is being broadly practiced, and that age is a key determinant for sexual concurrency.
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- Date Issued: 2016
Using bioinformatics tools to screen for trypanosomal cathepsin B cysteine protease inhibitors from the SANCDB as a novel therapeutic modality against Human African Trypanosomiasis (HAT)
- Authors: Mokhawa, Gaone
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3304 , vital:20470
- Description: Human African Trypanosomiasis (HAT), also known as sleeping sickness, is a fatal chronic disease that is caused by flagellated protozoans, Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense. HAT is spread by a bite from an infected tsetse fly of the Glosina genus. Up to 60 million people in 36 countries in sub-Saharan Africa are at a risk of infection from HAT with up to 30 000 deaths reported every year. Current chemotherapy for HAT is insufficient since the available drugs exhibit unacceptable side effects (toxicity) and parasite resistance. Novel treatments and approaches for development of specific and more potent drugs for HAT are therefore required. One approach is to target vital proteins that are essential to the life cycle of the parasite. The main interest of this study is to explore Trypanosoma brucei cathepsin B-like protease (TbCatB) structural and functional properties with the primary goal of discovering non peptide small molecule inhibitors of TbCatB using bioinformatics approaches. TbCatB is a papain family C1 cysteine protease which belongs to clan CA group and it has emerged as a potential HAT drug target. Papain family cysteine proteases of Clan CA group of Trypanosoma brucei (rhodesain and TbCatB) have demonstrated potential as chemotherapeutic targets using synthetic protease inhibitors like Z-Phe-Ala-CHN2 to kill the parasite in vitro and in vivo. TbCatB has been identified as the essential cysteine protease of T. brucei since mRNA silencing of TbCatB killed the parasite and resulted in a cure in mice infected with T. brucei while mRNA silencing of rhodesain only extended mice life. TbCatB is therefore a promising drug target against HAT and the discovery and development of compounds that can selectively inhibit TbCatB without posing any danger to the human host represent a great therapeutic solution for treatment of HAT. To understand protein-inhibitor interactions, useful information can be obtained from high resolution protease-inhibitor crystal structure complexes. This study aims to use bioinformatics approaches to carry out comparative sequence, structural and functional analysis of TbCatB protease and its homologs from T. congolense, T, cruzi, T. vivax and H. sapien as well as to identify non-peptide small molecule inhibitors of TbCatB cysteine proteases from natural compounds of South African origin. Sequences of TbCatB (PDB ID: 3HHI) homologs were retrieved by a BLAST search. Human cathepsin B (PDB ID: 3CBJ) was selected from a list of templates for homology modelling found by HHpred. MODELLER version 9.10 program was used to generate a hundred models for T. congolense, T, cruzi and T. vivax cathepsin B like proteases using 3HHI and 3CBJ as templates. The best models were chosen based on their low DOPE Z scores before validation using MetaMQAPII, ANOLEA, PROCHECK and QMEAN6. The DOPE Z scores and the RMSD (RMS) values of the calculated models indicate that the models are of acceptable energy (stability) and fold (conformation). Results from the different MQAPs indicate the models are of acceptable quality and they can be used for docking studies. High throughput screening of SANCDB using AutoDock Vina revealed nine compounds, SANC00 478, 479, 480, 481, 482, 488, 489, 490 and 491, having a strong affinity for Trypanosoma spp. cathepsin B proteases than HsCatB. SANC00488 has the strongest binding to Trypanosoma spp. cathepsin B proteases and the weakest binding to HsCatB protease. Molecular dynamics (MD) simulations show that the complexes between SANC00488 and TbCatB, TcCatB, TcrCatB and TvCatB are stable and do not come apart during simulation. The complex between this compound and HsCatB however is unstable and comes apart during simulation. Residues that are important for the stability of SANC00488-TbCatB complex are Gly328 of the S2 subsite, Phe208, and Ala256. In conclusion SANC00488 is a good candidate for development of a drug against HAT.
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- Date Issued: 2016