Prevalence and antibiotic resistance determinants of Escherichia coli pathotypes obtained from raw milk in two farms from the Eastern Cape, South Africa: public health implications
- Authors: Caine, Lesley-Anne
- Date: 2013
- Subjects: Raw Milk -- Escherichia coli , Polymerase -- Chain Reaction (PCR)
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11277 , http://hdl.handle.net/10353/d1015525 , Raw Milk -- Escherichia coli , Polymerase -- Chain Reaction (PCR)
- Description: Milk quality continues to be a topic of intense debate in the dairy industry, medical and public health communities. Production of maximum quantities of high-quality milk is an important goal of every dairy operation. High-quality milk must contain a low number of somatic cells and low bacteria count, and must be free of human pathogens and antibiotic residues. The objective of this study was to determine the prevalence of E. coli in unpasteurized milk recovered from Middledrift and Fort Hare dairy. In this study 400 milk samples were collected from two commercial farms (Middledrift and Fort Hare) in the Eastern Cape, South Africa, 200 raw milk samples from each farm. Samples were cultured on violet red bile mug-agar (VRB-MUG Agar) and incubated at 37ºC for 24 hours and preliminary identified by Gram stain and catalase test. Isolates that were Gram negative and catalase positive were screened for a marker of E. coli uidA gene using PCR assays. Middledrift dairy farm had 50 (25%) E. coli isolated from raw milk and Fort Hare farm showed 37 (18.5%) E. coli present in the milk samples. The presence of E. coli found in the milk samples points to the fact that fecal contamination was unavoidable and traditional practices are likely to contribute to the contamination of the milk and proliferation of the microorganisms.
- Full Text:
- Date Issued: 2013
- Authors: Caine, Lesley-Anne
- Date: 2013
- Subjects: Raw Milk -- Escherichia coli , Polymerase -- Chain Reaction (PCR)
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11277 , http://hdl.handle.net/10353/d1015525 , Raw Milk -- Escherichia coli , Polymerase -- Chain Reaction (PCR)
- Description: Milk quality continues to be a topic of intense debate in the dairy industry, medical and public health communities. Production of maximum quantities of high-quality milk is an important goal of every dairy operation. High-quality milk must contain a low number of somatic cells and low bacteria count, and must be free of human pathogens and antibiotic residues. The objective of this study was to determine the prevalence of E. coli in unpasteurized milk recovered from Middledrift and Fort Hare dairy. In this study 400 milk samples were collected from two commercial farms (Middledrift and Fort Hare) in the Eastern Cape, South Africa, 200 raw milk samples from each farm. Samples were cultured on violet red bile mug-agar (VRB-MUG Agar) and incubated at 37ºC for 24 hours and preliminary identified by Gram stain and catalase test. Isolates that were Gram negative and catalase positive were screened for a marker of E. coli uidA gene using PCR assays. Middledrift dairy farm had 50 (25%) E. coli isolated from raw milk and Fort Hare farm showed 37 (18.5%) E. coli present in the milk samples. The presence of E. coli found in the milk samples points to the fact that fecal contamination was unavoidable and traditional practices are likely to contribute to the contamination of the milk and proliferation of the microorganisms.
- Full Text:
- Date Issued: 2013
Rural households livelihoods' strategies and opportunities with regard to farming: a case of Intsika Yethu Local Municipality
- Authors: Gidi, Lungile Sivuyile
- Date: 2013
- Subjects: Poverty -- South Africa -- Eastern Cape , Agricultural productivity -- South Africa -- Eastern Cape , Community development -- South Africa -- Eastern Cape , Food security -- South Africa -- Eastern Cape , Rural development -- South Africa -- Eastern Cape , Households -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Qamata , Livelihood strategies , Irrigation farmers and crop , Livestock production
- Language: English
- Type: Thesis , Masters , MSc Agric (Agricultural Economics)
- Identifier: vital:11199 , http://hdl.handle.net/10353/d1007218 , Poverty -- South Africa -- Eastern Cape , Agricultural productivity -- South Africa -- Eastern Cape , Community development -- South Africa -- Eastern Cape , Food security -- South Africa -- Eastern Cape , Rural development -- South Africa -- Eastern Cape , Households -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Qamata , Livelihood strategies , Irrigation farmers and crop , Livestock production
- Description: South Africa and other developing countries are grappling with high levels of poverty as a result of slow to negligible rural development. Irrigation development is one essential component that can be used to address the challenges faced by smallholder farmers in rural areas. In the President‟s “State of the Nation Address” in 2011, the President of South Africa outlined measures to reduce hunger and poverty in communal areas through enhanced government‟s expenditures in rural infrastructure developments, especially for such amenities as irrigation and roads, with a view to promoting food security. The broad objective of this study is to assess livelihood strategies and opportunities with regard to farming in Qamata area of Intsika Yethu Local Municipality. The stratified random sampling method was applied in order to choose a sample of 70 household that were interviewed by means of semi-structured questionnaires. Out of these, 53 belonged to the irrigation project and 17 farmers were non-irrigation farmers. The results show that women play an active role in agriculture. The Multiple Regression model was used to assess the relative importance of different livelihood strategies adopted by both irrigation and non-irrigation farmers in improving household food security and welfare in Qamata. More specifically, the study assessed the impact of different livelihood strategies on production of butternut, goats, maize and poultry (Chicken) in Qamata. The results show increased agricultural production, crop diversification and higher incomes from irrigation farming as compared to dry land farming. Irrigation farming has enabled many households to diversify their sources of income and therefore include activities and enterprises that contribute to enhanced household welfare. The study showed that household size is crucial in crop production, followed by gender of the household head. The government and research institutes need to come up with programmes to train people on ways to produce crop and livestock products more efficiently.
- Full Text:
- Date Issued: 2013
- Authors: Gidi, Lungile Sivuyile
- Date: 2013
- Subjects: Poverty -- South Africa -- Eastern Cape , Agricultural productivity -- South Africa -- Eastern Cape , Community development -- South Africa -- Eastern Cape , Food security -- South Africa -- Eastern Cape , Rural development -- South Africa -- Eastern Cape , Households -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Qamata , Livelihood strategies , Irrigation farmers and crop , Livestock production
- Language: English
- Type: Thesis , Masters , MSc Agric (Agricultural Economics)
- Identifier: vital:11199 , http://hdl.handle.net/10353/d1007218 , Poverty -- South Africa -- Eastern Cape , Agricultural productivity -- South Africa -- Eastern Cape , Community development -- South Africa -- Eastern Cape , Food security -- South Africa -- Eastern Cape , Rural development -- South Africa -- Eastern Cape , Households -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Qamata , Livelihood strategies , Irrigation farmers and crop , Livestock production
- Description: South Africa and other developing countries are grappling with high levels of poverty as a result of slow to negligible rural development. Irrigation development is one essential component that can be used to address the challenges faced by smallholder farmers in rural areas. In the President‟s “State of the Nation Address” in 2011, the President of South Africa outlined measures to reduce hunger and poverty in communal areas through enhanced government‟s expenditures in rural infrastructure developments, especially for such amenities as irrigation and roads, with a view to promoting food security. The broad objective of this study is to assess livelihood strategies and opportunities with regard to farming in Qamata area of Intsika Yethu Local Municipality. The stratified random sampling method was applied in order to choose a sample of 70 household that were interviewed by means of semi-structured questionnaires. Out of these, 53 belonged to the irrigation project and 17 farmers were non-irrigation farmers. The results show that women play an active role in agriculture. The Multiple Regression model was used to assess the relative importance of different livelihood strategies adopted by both irrigation and non-irrigation farmers in improving household food security and welfare in Qamata. More specifically, the study assessed the impact of different livelihood strategies on production of butternut, goats, maize and poultry (Chicken) in Qamata. The results show increased agricultural production, crop diversification and higher incomes from irrigation farming as compared to dry land farming. Irrigation farming has enabled many households to diversify their sources of income and therefore include activities and enterprises that contribute to enhanced household welfare. The study showed that household size is crucial in crop production, followed by gender of the household head. The government and research institutes need to come up with programmes to train people on ways to produce crop and livestock products more efficiently.
- Full Text:
- Date Issued: 2013
Vegetation and soil characteristics around water points under three land management systems in semi-arid rangelands of the Eastern Cape, South Africa
- Authors: Simanga, Siyabulela
- Date: 2013
- Subjects: Livestock -- Effect of water quality on -- South Africa -- Eastern Cape , Biodiversity conservation -- South Africa -- Eastern Cape , Range ecology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Pasture Science)
- Identifier: vital:11584 , http://hdl.handle.net/10353/d1015795 , Livestock -- Effect of water quality on -- South Africa -- Eastern Cape , Biodiversity conservation -- South Africa -- Eastern Cape , Range ecology -- South Africa -- Eastern Cape
- Description: This study investigated the vegetation and soil characteristics in relation to distance from water points under different land management systems in semi-arid rangelands of South Africa. Six study sites, two each from communal grazing, commercial farming and game reserves were selected. Two watering points were selected in each study site. Two 500 m transects were laid from the selected watering points. Each transect was divided into sub-transects at 25 m, 50m, 100m, 200m, 300m and 500m from water points. A 100 m2 plot was marked in each sub-transect to record grass biomas, species composition, structure and distribution of woody vegetation and physical and chemical soil properties. Data were collected for two seasons 2012/13 (winter and summer). Thirty and 41 grass and woody species respectively were identified in all study areas. The most common and dominating grass species include Cynodon dactylon, Digitaria eriantha, Eragrostis obtusa, Setaria sphacelata and Sporobolus fimbriatus. Cynodon dactylon and S. sphacelata occurred more abundantly (p < 0.05) in the game reserves than in the other land management catergories. All the grass species had similar (p > 0.05) abundance along distance gradient from water points. Grass dry matter (GDM) showed significant differences (p < 0.05) between and within land management systems. However, GDM was not significantly affected by season, location of water point within each farm or reserve and distance along water points. Acacia karoo, Coddia rudis and Ehretia rigida were the most dominant woody species. Tree equivalent (TE) density of all encroaching woody plants combined was significantly (p < 0.05) higher on the communal area (1732 TE ha-1) than the commercial ranches (1136 TE ha-1) and game reserves (857 TE ha-1), but with no marked variations along distance from water points under all the land management systems. The electric conductivity (EC) was significantly (P < 0.01) higher in game reserves than in communal grazing areas and ranches. Soil organic matter percentage showed greatest and lowest values in the game reserves and commercial respectively. Soil pH and bulk density did not vary but soil organic matter (SOM), EC, bulk density and soil compaction were significant different with no increasing or decreasing trends. Soil properties were affected by herbivore pressure and trampling around water points with inconsistence magnitude and direction. In conclusion, grass species composition and GDM did not respond to distance from water points because either grazing gradient was absent or the length of transects was not enough to explain the absence or presence of gradients. High proportion of A. karoo and high densities of seedlings and saplings would seem as very good indicators of the woody vegetation changes in the different land management systems and distance from water points. The soil quality indicators around the water-points showed that livestock and game affected soil parameters.
- Full Text:
- Date Issued: 2013
- Authors: Simanga, Siyabulela
- Date: 2013
- Subjects: Livestock -- Effect of water quality on -- South Africa -- Eastern Cape , Biodiversity conservation -- South Africa -- Eastern Cape , Range ecology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Pasture Science)
- Identifier: vital:11584 , http://hdl.handle.net/10353/d1015795 , Livestock -- Effect of water quality on -- South Africa -- Eastern Cape , Biodiversity conservation -- South Africa -- Eastern Cape , Range ecology -- South Africa -- Eastern Cape
- Description: This study investigated the vegetation and soil characteristics in relation to distance from water points under different land management systems in semi-arid rangelands of South Africa. Six study sites, two each from communal grazing, commercial farming and game reserves were selected. Two watering points were selected in each study site. Two 500 m transects were laid from the selected watering points. Each transect was divided into sub-transects at 25 m, 50m, 100m, 200m, 300m and 500m from water points. A 100 m2 plot was marked in each sub-transect to record grass biomas, species composition, structure and distribution of woody vegetation and physical and chemical soil properties. Data were collected for two seasons 2012/13 (winter and summer). Thirty and 41 grass and woody species respectively were identified in all study areas. The most common and dominating grass species include Cynodon dactylon, Digitaria eriantha, Eragrostis obtusa, Setaria sphacelata and Sporobolus fimbriatus. Cynodon dactylon and S. sphacelata occurred more abundantly (p < 0.05) in the game reserves than in the other land management catergories. All the grass species had similar (p > 0.05) abundance along distance gradient from water points. Grass dry matter (GDM) showed significant differences (p < 0.05) between and within land management systems. However, GDM was not significantly affected by season, location of water point within each farm or reserve and distance along water points. Acacia karoo, Coddia rudis and Ehretia rigida were the most dominant woody species. Tree equivalent (TE) density of all encroaching woody plants combined was significantly (p < 0.05) higher on the communal area (1732 TE ha-1) than the commercial ranches (1136 TE ha-1) and game reserves (857 TE ha-1), but with no marked variations along distance from water points under all the land management systems. The electric conductivity (EC) was significantly (P < 0.01) higher in game reserves than in communal grazing areas and ranches. Soil organic matter percentage showed greatest and lowest values in the game reserves and commercial respectively. Soil pH and bulk density did not vary but soil organic matter (SOM), EC, bulk density and soil compaction were significant different with no increasing or decreasing trends. Soil properties were affected by herbivore pressure and trampling around water points with inconsistence magnitude and direction. In conclusion, grass species composition and GDM did not respond to distance from water points because either grazing gradient was absent or the length of transects was not enough to explain the absence or presence of gradients. High proportion of A. karoo and high densities of seedlings and saplings would seem as very good indicators of the woody vegetation changes in the different land management systems and distance from water points. The soil quality indicators around the water-points showed that livestock and game affected soil parameters.
- Full Text:
- Date Issued: 2013
- «
- ‹
- 1
- ›
- »