BODIPY dyes for application in the photo-oxidation of pollutants, photodynamic antimicrobial chemotherapy, and nonlinear optics
- Authors: Kelechi, Lebechi Augustus
- Date: 2020
- Subjects: Dyes and dyeing -- Chemistry , Fluorescent probes , Fluorescence spectroscopy
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/140298 , vital:37859
- Description: The synthesis and structural characterization of a series of BODIPY dyes to analyze both the effects of halogenations at the 2,6-positions and the introduction of styryl groups at the 3,5-positions. The photophysical properties of these dyes were investigated to determine their suitability as singlet oxygen-generating photosensitiser dyes for application in photocatalytic degradation of azo dyes and in photodynamic antimicrobial chemotherapy (PACT). Upon halogenation, the dyes showed high to moderate singlet oxygen quantum yields. The potential utility of electrospun polystyrene (PS) nanofibres embedded with halogenated BODIPY dyes for the photocatalytic degradation of Orange G and Methyl Orange from textile industry effluents were investigated. A comparison of the singlet oxygen quantum yield of the BODIPY dyes in solution and when embedded in the PS nanofibres support demonstrates that its photosensitiser properties are maintained in the nanofibre mats. The photocatalytic degradation properties of the PS nanofibres for Orange G and Methyl Orange were determined by using a 530 nm and 660 nm light-emitting diodes. The rate of photodegradation increases with both the Orange G and Methyl Orange concentrations and follows pseudo-first-order kinetics. The PACT activities of brominated BODIPYs on Escherichia coli and Staphylococcus aureus were investigated. Log reduction values of over 9 were obtained during the photoinactivation of Staphylococcus aureus. To be able to red-shift the main spectral band of the BODIPY dyes into the therapeutic window, styryl groups were introduced at the 3,5-positions through a modified Knoevenagel condensation reaction. Because the red-shifted spectral band lies above 532 nm, the second harmonic of the Nd:YAG laser, there is very minute absorption at this wavelength. One of the novel brominated BODIPY dyes was investigated for its potential utility as optical limiting materials in nonlinear optics (NLO), and the dyes demonstrated typical nonlinear absorption behaviour characterised by reverse saturable absorption (RSA) in Z-scan measurements. Excellent optical limiting parameters were obtained for third-order susceptibility and hyperpolarisability.
- Full Text:
- Date Issued: 2020
- Authors: Kelechi, Lebechi Augustus
- Date: 2020
- Subjects: Dyes and dyeing -- Chemistry , Fluorescent probes , Fluorescence spectroscopy
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/140298 , vital:37859
- Description: The synthesis and structural characterization of a series of BODIPY dyes to analyze both the effects of halogenations at the 2,6-positions and the introduction of styryl groups at the 3,5-positions. The photophysical properties of these dyes were investigated to determine their suitability as singlet oxygen-generating photosensitiser dyes for application in photocatalytic degradation of azo dyes and in photodynamic antimicrobial chemotherapy (PACT). Upon halogenation, the dyes showed high to moderate singlet oxygen quantum yields. The potential utility of electrospun polystyrene (PS) nanofibres embedded with halogenated BODIPY dyes for the photocatalytic degradation of Orange G and Methyl Orange from textile industry effluents were investigated. A comparison of the singlet oxygen quantum yield of the BODIPY dyes in solution and when embedded in the PS nanofibres support demonstrates that its photosensitiser properties are maintained in the nanofibre mats. The photocatalytic degradation properties of the PS nanofibres for Orange G and Methyl Orange were determined by using a 530 nm and 660 nm light-emitting diodes. The rate of photodegradation increases with both the Orange G and Methyl Orange concentrations and follows pseudo-first-order kinetics. The PACT activities of brominated BODIPYs on Escherichia coli and Staphylococcus aureus were investigated. Log reduction values of over 9 were obtained during the photoinactivation of Staphylococcus aureus. To be able to red-shift the main spectral band of the BODIPY dyes into the therapeutic window, styryl groups were introduced at the 3,5-positions through a modified Knoevenagel condensation reaction. Because the red-shifted spectral band lies above 532 nm, the second harmonic of the Nd:YAG laser, there is very minute absorption at this wavelength. One of the novel brominated BODIPY dyes was investigated for its potential utility as optical limiting materials in nonlinear optics (NLO), and the dyes demonstrated typical nonlinear absorption behaviour characterised by reverse saturable absorption (RSA) in Z-scan measurements. Excellent optical limiting parameters were obtained for third-order susceptibility and hyperpolarisability.
- Full Text:
- Date Issued: 2020
Developing social indicators for the evaluation of natural resource management programmes using a capability approach in the Eastern Cape, South Africa
- Authors: Human, Johanna Susanna
- Date: 2020
- Subjects: Environmental education -- South Africa -- Eastern Cape , Natural resources -- Management -- Study and teaching (Continuing education) -- South Africa -- Eastern Cape , Sustainable development -- Study and teaching (Continuing education) -- South Africa -- Eastern Cape , Environmental economics -- Study and teaching (Continuing education) -- South Africa -- Eastern Cape , Subsistence farming -- South Africa -- Eastern Cape , Watersheds -- South Africa -- Eastern Cape , Capabilities approach (Social sciences) , Tsitsa River Catchment (Eastern Cape, South Africa)
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/147348 , vital:38628
- Description: The dissertation investigates how the capability approach can be used to develop social indicators for a natural resource management (NRM) programme. The study aimed to develop an understanding of what the beneficiaries of the programme value being and doing to guide the development of social indicators that will take into consideration their personal development aspirations. These valued beings and doings were then overlaid with the programme goals and objectives to identify and select the final indicators. The study was situated in the Tsitsa catchment in the Eastern Cape, South Africa and was part of a natural resource management programme, focused on land restoration and avoided degradation. Methods used to collect data for this study and develop an understanding of what the residents’ valued beings and doings were, included semi-structured interviews, workshops and document analysis. The research population was non-homogenous. I tried to include voices representing different prominent groups of land users in the study. This included established commercial farmers, emerging commercial farmers, commercial forestry, residents residing in communal areas and engaged with subsistence farming and residents from these communal areas who are now residing elsewhere but continue to have connections to the catchment. Understanding the voices of these different groups who reside in the catchment was important to develop an inclusive understanding of what residents of the catchment valued beings and doings were. The research process was supported by participatory approaches. This process, and the lessons learnt during the research process, were valuable for the further development and implementation of the planned participatory monitoring evaluation reflection and learning (PMERL) framework. The research process involved learning from the research participants about their valued beings and doings and what was important for the programme implementers. Some of the research participants from the Rhodes University implementation team (RUIT) were also involved in selecting the indicators they thought the programme should monitor. Overall, using the capability approach helped me to gain insight and understanding with regard to what the valued beings and doings are for the residents of the catchment and what they would like development programmes, such as the Tsitsa Project, to address. It was surprising to find many areas of overlap between the valued beings and doings of the different residents in the catchment, as well as the similarities between these and the goals and objectives of the Tsitsa Project. In conclusion, using the capability approach was useful to learn what was important for the residents of the catchment to help develop the social indicators but also to learn how to guide and steer participatory monitoring and evaluation processes in the future.
- Full Text:
- Date Issued: 2020
- Authors: Human, Johanna Susanna
- Date: 2020
- Subjects: Environmental education -- South Africa -- Eastern Cape , Natural resources -- Management -- Study and teaching (Continuing education) -- South Africa -- Eastern Cape , Sustainable development -- Study and teaching (Continuing education) -- South Africa -- Eastern Cape , Environmental economics -- Study and teaching (Continuing education) -- South Africa -- Eastern Cape , Subsistence farming -- South Africa -- Eastern Cape , Watersheds -- South Africa -- Eastern Cape , Capabilities approach (Social sciences) , Tsitsa River Catchment (Eastern Cape, South Africa)
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/147348 , vital:38628
- Description: The dissertation investigates how the capability approach can be used to develop social indicators for a natural resource management (NRM) programme. The study aimed to develop an understanding of what the beneficiaries of the programme value being and doing to guide the development of social indicators that will take into consideration their personal development aspirations. These valued beings and doings were then overlaid with the programme goals and objectives to identify and select the final indicators. The study was situated in the Tsitsa catchment in the Eastern Cape, South Africa and was part of a natural resource management programme, focused on land restoration and avoided degradation. Methods used to collect data for this study and develop an understanding of what the residents’ valued beings and doings were, included semi-structured interviews, workshops and document analysis. The research population was non-homogenous. I tried to include voices representing different prominent groups of land users in the study. This included established commercial farmers, emerging commercial farmers, commercial forestry, residents residing in communal areas and engaged with subsistence farming and residents from these communal areas who are now residing elsewhere but continue to have connections to the catchment. Understanding the voices of these different groups who reside in the catchment was important to develop an inclusive understanding of what residents of the catchment valued beings and doings were. The research process was supported by participatory approaches. This process, and the lessons learnt during the research process, were valuable for the further development and implementation of the planned participatory monitoring evaluation reflection and learning (PMERL) framework. The research process involved learning from the research participants about their valued beings and doings and what was important for the programme implementers. Some of the research participants from the Rhodes University implementation team (RUIT) were also involved in selecting the indicators they thought the programme should monitor. Overall, using the capability approach helped me to gain insight and understanding with regard to what the valued beings and doings are for the residents of the catchment and what they would like development programmes, such as the Tsitsa Project, to address. It was surprising to find many areas of overlap between the valued beings and doings of the different residents in the catchment, as well as the similarities between these and the goals and objectives of the Tsitsa Project. In conclusion, using the capability approach was useful to learn what was important for the residents of the catchment to help develop the social indicators but also to learn how to guide and steer participatory monitoring and evaluation processes in the future.
- Full Text:
- Date Issued: 2020
Technology in conservation: towards a system for in-field drone detection of invasive vegetation
- James, Katherine Margaret Frances
- Authors: James, Katherine Margaret Frances
- Date: 2020
- Subjects: Drone aircraft in remote sensing , Neural networks (Computer science) , Drone aircraft in remote sensing -- Case studies , Machine learning , Computer vision , Environmental monitoring -- Remote sensing , Invasive plants -- Monitoring
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/143408 , vital:38244
- Description: Remote sensing can assist in monitoring the spread of invasive vegetation. The adoption of camera-carrying unmanned aerial vehicles, commonly referred to as drones, as remote sensing tools has yielded images of higher spatial resolution than traditional techniques. Drones also have the potential to interact with the environment through the delivery of bio-control or herbicide, as seen with their adoption in precision agriculture. Unlike in agricultural applications, however, invasive plants do not have a predictable position relative to each other within the environment. To facilitate the adoption of drones as an environmental monitoring and management tool, drones need to be able to intelligently distinguish between invasive and non-invasive vegetation on the fly. In this thesis, we present the augmentation of a commercially available drone with a deep machine learning model to investigate the viability of differentiating between an invasive shrub and other vegetation. As a case study, this was applied to the shrub genus Hakea, originating in Australia and invasive in several countries including South Africa. However, for this research, the methodology is important, rather than the chosen target plant. A dataset was collected using the available drone and manually annotated to facilitate the supervised training of the model. Two approaches were explored, namely, classification and semantic segmentation. For each of these, several models were trained and evaluated to find the optimal one. The chosen model was then interfaced with the drone via an Android application on a mobile device and its performance was preliminarily evaluated in the field. Based on these findings, refinements were made and thereafter a thorough field evaluation was performed to determine the best conditions for model operation. Results from the classification task show that deep learning models are capable of distinguishing between target and other shrubs in ideal candidate windows. However, classification in this manner is restricted by the proposal of such candidate windows. End-to-end image segmentation using deep learning overcomes this problem, classifying the image in a pixel-wise manner. Furthermore, the use of appropriate loss functions was found to improve model performance. Field tests show that illumination and shadow pose challenges to the model, but that good recall can be achieved when the conditions are ideal. False positive detection remains an issue that could be improved. This approach shows the potential for drones as an environmental monitoring and management tool when coupled with deep machine learning techniques and outlines potential problems that may be encountered.
- Full Text:
- Date Issued: 2020
- Authors: James, Katherine Margaret Frances
- Date: 2020
- Subjects: Drone aircraft in remote sensing , Neural networks (Computer science) , Drone aircraft in remote sensing -- Case studies , Machine learning , Computer vision , Environmental monitoring -- Remote sensing , Invasive plants -- Monitoring
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/143408 , vital:38244
- Description: Remote sensing can assist in monitoring the spread of invasive vegetation. The adoption of camera-carrying unmanned aerial vehicles, commonly referred to as drones, as remote sensing tools has yielded images of higher spatial resolution than traditional techniques. Drones also have the potential to interact with the environment through the delivery of bio-control or herbicide, as seen with their adoption in precision agriculture. Unlike in agricultural applications, however, invasive plants do not have a predictable position relative to each other within the environment. To facilitate the adoption of drones as an environmental monitoring and management tool, drones need to be able to intelligently distinguish between invasive and non-invasive vegetation on the fly. In this thesis, we present the augmentation of a commercially available drone with a deep machine learning model to investigate the viability of differentiating between an invasive shrub and other vegetation. As a case study, this was applied to the shrub genus Hakea, originating in Australia and invasive in several countries including South Africa. However, for this research, the methodology is important, rather than the chosen target plant. A dataset was collected using the available drone and manually annotated to facilitate the supervised training of the model. Two approaches were explored, namely, classification and semantic segmentation. For each of these, several models were trained and evaluated to find the optimal one. The chosen model was then interfaced with the drone via an Android application on a mobile device and its performance was preliminarily evaluated in the field. Based on these findings, refinements were made and thereafter a thorough field evaluation was performed to determine the best conditions for model operation. Results from the classification task show that deep learning models are capable of distinguishing between target and other shrubs in ideal candidate windows. However, classification in this manner is restricted by the proposal of such candidate windows. End-to-end image segmentation using deep learning overcomes this problem, classifying the image in a pixel-wise manner. Furthermore, the use of appropriate loss functions was found to improve model performance. Field tests show that illumination and shadow pose challenges to the model, but that good recall can be achieved when the conditions are ideal. False positive detection remains an issue that could be improved. This approach shows the potential for drones as an environmental monitoring and management tool when coupled with deep machine learning techniques and outlines potential problems that may be encountered.
- Full Text:
- Date Issued: 2020
Understanding of the underlying resistance mechanism of the Kat-G protein against isoniazid in Mycobacterium tuberculosis using bioinformatics approaches
- Authors: Barozi, Victor
- Date: 2020
- Subjects: Mycobacterium tuberculosis , Isoniazid , Drug resistance in microorganisms , Proteins -- Microbiology
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/146592 , vital:38540
- Description: Tuberculosis (TB) is a multi-organ infection caused by rod-shaped acid-fast Mycobacterium tuberculosis. The World Health Organization (WHO) ranks TB among the top 10 fatal infections and the leading the cause of death from a single infection. In 2017, TB was responsible for an estimated 1.3 million deaths among both the HIV negative and positive populations worldwide (WHO, 2018). Approximately 23% (roughly 1.7 billion) of the world’s population is estimated to have latent TB with a high risk of reverting to active TB infection. In 2017, an estimated 558,000 people developed drug resistant TB worldwide with 82% of the cases being multi-drug resistant TB (WHO, 2018). South Africa is ranked among the 30 high TB burdened countries with a TB incidence of 322,000 cases in 2017 accounting for 3% of the world’s TB cases. TB is curable and is clinically managed through a combination of intensive and continuation phases of first-line drugs (isoniazid, rifampicin, ethambutol, and pyrazinamide). Second-line drugs which include fluoroquinolones, injectable aminoglycoside and injectable polypeptides are used in cases of first line drug resistance. The third-line drugs include amoxicillin, clofazimine, linezolid and imipenem. These have variable but unproven efficacy to TB and are the last resort in cases of total drug resistance (Jilani et al., 2019). TB drug resistance to first-line drugs especially isoniazid in M. tuberculosis has been attributed to single nucleotide polymorphisms (SNPs) in the catalase peroxidase enzyme (katG), a protein important in the activation of the pro-drug isoniazid. The SNPs especially at position 315 of the katG enzyme are believed to reduce the sensitivity of the M. tuberculosis to isoniazid while still maintaining the enzyme’s catalytic activity - a mechanism not completely understood. KatG protein is important for protecting the bacteria from hydro peroxides and hydroxyl radicals present in an aerobic environment. This study focused on understanding the mechanism of isoniazid drug resistance in M. tuberculosis as a result of high confidence mutations in the katG through modelling the enzyme with its respective variants, performing MD simulations to explore the protein behaviour, calculating the dynamic residue network analysis (DRN) of the variants in respect to the wild type katG and finally performing alanine scanning. From the MD simulations, it was observed that the high confidence mutations i.e. S140R, S140N, G279D, G285D, S315T, S315I, S315R, S315N, G316D, S457I and G593D were not only reducing the backbone flexibility of the protein but also reducing the protein’s conformational variation and space. All the variant protein structures were observed to be more compact compared to the wild type. Residue fluctuation results indicated reduced residue flexibility across all variants in the loop region (position 26-110) responsible for katG dimerization. In addition, mutation S315T is believed to reduce the size of the active site access channel in the protein. From the DRN data, residues in the interface region between the N and C-terminal domains were observed to gain importance in the variants irrespective of the mutation location indicating an allosteric effect of the mutations on the interface region. Alanine scanning results established that residue Leucine at position 48 was not only important in the protein communication but also a destabilizing residue across all the variants. The study not only demonstrated change in the protein behaviour but also showed allosteric effect of the mutations in the katG protein.
- Full Text:
- Date Issued: 2020
- Authors: Barozi, Victor
- Date: 2020
- Subjects: Mycobacterium tuberculosis , Isoniazid , Drug resistance in microorganisms , Proteins -- Microbiology
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/146592 , vital:38540
- Description: Tuberculosis (TB) is a multi-organ infection caused by rod-shaped acid-fast Mycobacterium tuberculosis. The World Health Organization (WHO) ranks TB among the top 10 fatal infections and the leading the cause of death from a single infection. In 2017, TB was responsible for an estimated 1.3 million deaths among both the HIV negative and positive populations worldwide (WHO, 2018). Approximately 23% (roughly 1.7 billion) of the world’s population is estimated to have latent TB with a high risk of reverting to active TB infection. In 2017, an estimated 558,000 people developed drug resistant TB worldwide with 82% of the cases being multi-drug resistant TB (WHO, 2018). South Africa is ranked among the 30 high TB burdened countries with a TB incidence of 322,000 cases in 2017 accounting for 3% of the world’s TB cases. TB is curable and is clinically managed through a combination of intensive and continuation phases of first-line drugs (isoniazid, rifampicin, ethambutol, and pyrazinamide). Second-line drugs which include fluoroquinolones, injectable aminoglycoside and injectable polypeptides are used in cases of first line drug resistance. The third-line drugs include amoxicillin, clofazimine, linezolid and imipenem. These have variable but unproven efficacy to TB and are the last resort in cases of total drug resistance (Jilani et al., 2019). TB drug resistance to first-line drugs especially isoniazid in M. tuberculosis has been attributed to single nucleotide polymorphisms (SNPs) in the catalase peroxidase enzyme (katG), a protein important in the activation of the pro-drug isoniazid. The SNPs especially at position 315 of the katG enzyme are believed to reduce the sensitivity of the M. tuberculosis to isoniazid while still maintaining the enzyme’s catalytic activity - a mechanism not completely understood. KatG protein is important for protecting the bacteria from hydro peroxides and hydroxyl radicals present in an aerobic environment. This study focused on understanding the mechanism of isoniazid drug resistance in M. tuberculosis as a result of high confidence mutations in the katG through modelling the enzyme with its respective variants, performing MD simulations to explore the protein behaviour, calculating the dynamic residue network analysis (DRN) of the variants in respect to the wild type katG and finally performing alanine scanning. From the MD simulations, it was observed that the high confidence mutations i.e. S140R, S140N, G279D, G285D, S315T, S315I, S315R, S315N, G316D, S457I and G593D were not only reducing the backbone flexibility of the protein but also reducing the protein’s conformational variation and space. All the variant protein structures were observed to be more compact compared to the wild type. Residue fluctuation results indicated reduced residue flexibility across all variants in the loop region (position 26-110) responsible for katG dimerization. In addition, mutation S315T is believed to reduce the size of the active site access channel in the protein. From the DRN data, residues in the interface region between the N and C-terminal domains were observed to gain importance in the variants irrespective of the mutation location indicating an allosteric effect of the mutations on the interface region. Alanine scanning results established that residue Leucine at position 48 was not only important in the protein communication but also a destabilizing residue across all the variants. The study not only demonstrated change in the protein behaviour but also showed allosteric effect of the mutations in the katG protein.
- Full Text:
- Date Issued: 2020
The relative roles of ecosystem services and disservices in rural livelihoods in the Eastern Cape, South Africa
- Authors: Herd-Hoare, Shannon
- Date: 2019
- Subjects: Ecosystem services -- Health aspects -- South Africa , South Africa -- Rural conditions , Biodiversity -- South Africa -- Health aspects , Rural development -- South Africa -- Health aspects
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/94080 , vital:30998
- Description: Since the publication of the Millennium Ecosystem Assessment report there has been a marked increase in research into various aspects of ecosystem services (ES). While ES are essential to human wellbeing, the literature has overlooked that some ecosystem goods and services also undermine human wellbeing. These are known as Ecosystem Disservices (EDS). This study aims to counter this imbalance in research, specifically in the context of rural livelihoods which are frequently dependent on local ES but often ill-equipped to manage EDS. The objectives of this study, conducted in three rural villages along a gradient of habitat diversity, were to identify a range of ES and EDS, determine their perceived contributions to or effects on the wellbeing of respondents, and identify modifications in livelihood strategies in response to EDS. The research made use of household surveys in conjunction with participatory learning and action techniques, such as focus group discussions in which ranking and trendline exercises, timelines, and participatory mapping were conducted. Findings indicated that although numerous participants were involved in the harvest of multiple ES, such as Non-Timber Forest Products (NTFPs), livestock outputs, crops and marine wildlife, they simultaneously experienced losses from EDS, such as ticks, unpalatable grass species, monkeys, and Lantana camara invasion which undermined livelihoods to varying degrees. The role of ES and EDS in livelihoods was expressed in economic terms, representing a common framework with which to illustrate the magnitude of their contribution or loss. For example, the average value of livestock goods and services, amongst those who owned and used outputs, was highest in the least biodiverse village at R9 753 per annum, while the corresponding value of potential livestock goods and services lost due to EDS, despite active implementation of prevention measures, totalled R22 426. Further, the average value of cultivated plots in the most biodiverse village could have totalled approximately R20 958, but because of EDS, represented less than 20% of this value. Further, the findings highlighted that the most biodiverse village also had the highest number of EDS, supporting Dunn (2010) who hypothesised that the number of EDS increases with the number of ES. Based on these findings, I propose that a comprehensive framework which systematically contextualises both the positive and negative contributions of ES is needed to grasp the full picture of how local people conceive and engage with nature to facilitate an understanding of the resulting practices and processes.
- Full Text:
- Date Issued: 2019
- Authors: Herd-Hoare, Shannon
- Date: 2019
- Subjects: Ecosystem services -- Health aspects -- South Africa , South Africa -- Rural conditions , Biodiversity -- South Africa -- Health aspects , Rural development -- South Africa -- Health aspects
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/94080 , vital:30998
- Description: Since the publication of the Millennium Ecosystem Assessment report there has been a marked increase in research into various aspects of ecosystem services (ES). While ES are essential to human wellbeing, the literature has overlooked that some ecosystem goods and services also undermine human wellbeing. These are known as Ecosystem Disservices (EDS). This study aims to counter this imbalance in research, specifically in the context of rural livelihoods which are frequently dependent on local ES but often ill-equipped to manage EDS. The objectives of this study, conducted in three rural villages along a gradient of habitat diversity, were to identify a range of ES and EDS, determine their perceived contributions to or effects on the wellbeing of respondents, and identify modifications in livelihood strategies in response to EDS. The research made use of household surveys in conjunction with participatory learning and action techniques, such as focus group discussions in which ranking and trendline exercises, timelines, and participatory mapping were conducted. Findings indicated that although numerous participants were involved in the harvest of multiple ES, such as Non-Timber Forest Products (NTFPs), livestock outputs, crops and marine wildlife, they simultaneously experienced losses from EDS, such as ticks, unpalatable grass species, monkeys, and Lantana camara invasion which undermined livelihoods to varying degrees. The role of ES and EDS in livelihoods was expressed in economic terms, representing a common framework with which to illustrate the magnitude of their contribution or loss. For example, the average value of livestock goods and services, amongst those who owned and used outputs, was highest in the least biodiverse village at R9 753 per annum, while the corresponding value of potential livestock goods and services lost due to EDS, despite active implementation of prevention measures, totalled R22 426. Further, the average value of cultivated plots in the most biodiverse village could have totalled approximately R20 958, but because of EDS, represented less than 20% of this value. Further, the findings highlighted that the most biodiverse village also had the highest number of EDS, supporting Dunn (2010) who hypothesised that the number of EDS increases with the number of ES. Based on these findings, I propose that a comprehensive framework which systematically contextualises both the positive and negative contributions of ES is needed to grasp the full picture of how local people conceive and engage with nature to facilitate an understanding of the resulting practices and processes.
- Full Text:
- Date Issued: 2019
Policy responses to the sexual and reproductive health of queer youth in the global south: a systematic review
- Authors: Moore, Sarah-Ann
- Date: 2018
- Subjects: Sexual minorities -- Africa , Sexual minorities -- Asia , Sexual minorities -- Caribbean Area , Sexual health -- Developing countries , Reproductive health -- Developing countries , Reproductive health services -- Developing countries , Communication in reproductive health -- Developing countries , Sexual minorities -- Youth -- Developing countries , Medical policy -- Developing countries , Homophobia -- Developing countries
- Language: English
- Type: text , Thesis , Masters , MSocSc
- Identifier: http://hdl.handle.net/10962/63021 , vital:28355
- Description: Concerns surrounding youth sexual and reproduction health (SRH) are deeply embedded within systems of heteronormativity and ciscentrism. Resultantly, youth SRH is filtered through a lens of heterosexual and cisgender experience, rendering invisible the SRH needs of queer youth. Importantly, a failure to recognise queer experiences of SRH has implications for normative subject positions, which enjoy stronger institutional support and constitute legitimate ways of being. As such, the failure to recognise queer youth as health care subjects within policy has far reaching consequences for their sexual and reproductive health. Within this research, a sexual and reproductive justice (SRJ) framework is adopted as a backdrop for exploring policy documents related to youth SRH within selected global South countries in Africa, Asia, and the Caribbean. The final data set comprises of 1035 policy excerpts extracted from 152 policies across these three regions. Research takes the form of a systematic review utilising a deductive framing and positioning thematic analysis. Analysis identifies framings of youth SRH and explores the subject positions assigned to queer youth in relation to these identified framings, with the understanding that the manner in which youth SRH concerns are framed and queer youth are positioned within policies provides an important foundation for the implementation of SRH-related policy. Findings demonstrate that policy responses to youth SRH are most often framed in terms of a public health approach. As a result, dominant understandings of youth SRH serve to reduce youth sexuality to notions of infections and impact, which may speak to an overreliance on biomedical and population-level health models. Themes emerging within human rights framings demonstrate a presumption that rights are equally afforded to, and freely exercised by, all individuals once legally secured, failing to engage with the creation of enabling conditions to realise these rights. Although context and culture framings were by no means exhaustive examples of SRJ, they provide an interesting insight into how such SRJ concerns might be integrated into policy. Importantly, policy responses demonstrate a general pattern hypervisibility of men who have sex with men (MSM) standing in marked contrast to the invisibility of queer youth and other adult queer populations. Within policy extracts, both youth and „MSM‟ are positioned as particularly prone to poor SRH outcomes. By virtue of their inclusion within both populations, queer youth may be considered as especially at risk for, or vulnerable to, such outcomes. Relatedly, these populations (and by extension queer youth) are positioned as in need of correction, containment, and/or protection by those occupying „gatekeeping‟ positions (e.g. health care providers). The positioning of „MSM‟ solely within the context of HIV/AIDS serves to link same-sex sexualities (and at times gender non-conformity) with harmful consequences, suggesting that the positioning of queer youth could similarly serve to conflate their SRH needs with concerns around HIV/AIDS. Many of the subject positions deployed in policies serve to deny the potential for youth and „MSM‟ agency, strength, and resilience. Thus, queer youth subjects are unlikely to be positioned as empowered, autonomous, and agentic. Across both framing and positioning themes, a number of key shortcomings were observed. For the most part, policy responses fail to acknowledge the influence of social, economic, political, and cultural forces that may serve to hinder SRH outcomes according to particular contexts and the intersection of multiple and varied social identities. By obscuring these broader contextual factors and power relations, policy responses may serve to hold individual youth responsible for poor SRH outcomes. In failing to engage with the potential for diversity within youth populations, these populations are largely homogenised. Finally, the need for the creation of an enabling environment in order to secure sexual and reproductive health is largely unacknowledged within policy responses.
- Full Text:
- Date Issued: 2018
- Authors: Moore, Sarah-Ann
- Date: 2018
- Subjects: Sexual minorities -- Africa , Sexual minorities -- Asia , Sexual minorities -- Caribbean Area , Sexual health -- Developing countries , Reproductive health -- Developing countries , Reproductive health services -- Developing countries , Communication in reproductive health -- Developing countries , Sexual minorities -- Youth -- Developing countries , Medical policy -- Developing countries , Homophobia -- Developing countries
- Language: English
- Type: text , Thesis , Masters , MSocSc
- Identifier: http://hdl.handle.net/10962/63021 , vital:28355
- Description: Concerns surrounding youth sexual and reproduction health (SRH) are deeply embedded within systems of heteronormativity and ciscentrism. Resultantly, youth SRH is filtered through a lens of heterosexual and cisgender experience, rendering invisible the SRH needs of queer youth. Importantly, a failure to recognise queer experiences of SRH has implications for normative subject positions, which enjoy stronger institutional support and constitute legitimate ways of being. As such, the failure to recognise queer youth as health care subjects within policy has far reaching consequences for their sexual and reproductive health. Within this research, a sexual and reproductive justice (SRJ) framework is adopted as a backdrop for exploring policy documents related to youth SRH within selected global South countries in Africa, Asia, and the Caribbean. The final data set comprises of 1035 policy excerpts extracted from 152 policies across these three regions. Research takes the form of a systematic review utilising a deductive framing and positioning thematic analysis. Analysis identifies framings of youth SRH and explores the subject positions assigned to queer youth in relation to these identified framings, with the understanding that the manner in which youth SRH concerns are framed and queer youth are positioned within policies provides an important foundation for the implementation of SRH-related policy. Findings demonstrate that policy responses to youth SRH are most often framed in terms of a public health approach. As a result, dominant understandings of youth SRH serve to reduce youth sexuality to notions of infections and impact, which may speak to an overreliance on biomedical and population-level health models. Themes emerging within human rights framings demonstrate a presumption that rights are equally afforded to, and freely exercised by, all individuals once legally secured, failing to engage with the creation of enabling conditions to realise these rights. Although context and culture framings were by no means exhaustive examples of SRJ, they provide an interesting insight into how such SRJ concerns might be integrated into policy. Importantly, policy responses demonstrate a general pattern hypervisibility of men who have sex with men (MSM) standing in marked contrast to the invisibility of queer youth and other adult queer populations. Within policy extracts, both youth and „MSM‟ are positioned as particularly prone to poor SRH outcomes. By virtue of their inclusion within both populations, queer youth may be considered as especially at risk for, or vulnerable to, such outcomes. Relatedly, these populations (and by extension queer youth) are positioned as in need of correction, containment, and/or protection by those occupying „gatekeeping‟ positions (e.g. health care providers). The positioning of „MSM‟ solely within the context of HIV/AIDS serves to link same-sex sexualities (and at times gender non-conformity) with harmful consequences, suggesting that the positioning of queer youth could similarly serve to conflate their SRH needs with concerns around HIV/AIDS. Many of the subject positions deployed in policies serve to deny the potential for youth and „MSM‟ agency, strength, and resilience. Thus, queer youth subjects are unlikely to be positioned as empowered, autonomous, and agentic. Across both framing and positioning themes, a number of key shortcomings were observed. For the most part, policy responses fail to acknowledge the influence of social, economic, political, and cultural forces that may serve to hinder SRH outcomes according to particular contexts and the intersection of multiple and varied social identities. By obscuring these broader contextual factors and power relations, policy responses may serve to hold individual youth responsible for poor SRH outcomes. In failing to engage with the potential for diversity within youth populations, these populations are largely homogenised. Finally, the need for the creation of an enabling environment in order to secure sexual and reproductive health is largely unacknowledged within policy responses.
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- Date Issued: 2018
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