Abortion Services and Reproductive Justice in Rural South Africa
- Authors: du Plessis, Ulandi , Macleod, Catriona I
- Date: 2023
- Subjects: To be catalogued
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/434081 , vital:73030 , ISBN 9781776148738 , https://www.witspress.co.za/page/detail/Abortion-Services-and-Reproductive-Justice-in-Rural-South-Africa/?K=9781776148776
- Description: Despite progressive legislation, abortion service implementa-tion and access in South Africa’s rural areas is challenging and directly affects low-income communities. This book urges an intervention for safe and accessible abortion services that does not compromise costs or confidentiality within a repara-tive reproductive justice framework. South Africa’s progressive abortion legislation was hailed as transformative in terms of reproductive health and rights. Despite this promise, many challenges persist resulting in a lack of services, especially in rural areas where distances and transport costs are a factor.
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- Date Issued: 2023
Policy and practice of inclusive education for mainstream secondary teachers supporting learners with physical disabilities in the Lower Shire, Malawi
- Authors: De Souza, Ben
- Date: 2021
- Subjects: Children with disabilities -- Education (Secondary) -- Malawi , Children with disabilities -- Education -- Government policy -- Malawi , Children with disabilities -- Legal status, laws, etc. -- Malawi , Inclusive education -- Malawi , Teachers -- Training of -- Malawi , High School teachers -- Malawi -- Attitudes
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/171254 , vital:42038
- Description: The Government of Malawi and its education partners are currently implementing the National Education Policy (2016) and the National Strategy on Inclusive Education (2017-2021). My study explored how mainstream secondary teachers interpret policies and strategies, and transition to the practice of supporting learners with physical disabilities. Previous studies revealed policy-to-practice disparities in the Malawian inclusive secondary education but did not proffer teacher-oriented strategies that can mitigate the disparities and enhance inclusive education. My study argued that effective strategies that could lessen policy-to-practice mismatches and advance inclusive education primarily rest with mainstream teachers, as they mediate policy and practice. This study drew on Clough and Corbett’s disability studies critique and Urie Bronfenbrenner’s bioecological systems perspective to form theoretical and analytical frameworks for understanding the problem and discussing the findings. The study generated primary data from the Lower Shire districts of Chikwawa and Nsanje in Malawi. Thirty-three teachers, randomly selected from four schools, responded to questionnaires. In-depth, face to face interviews were conducted with purposely selected teachers from the same four schools. The study also generated secondary data from a review of policy documents. The findings showed that policy directives are shifting inclusive education from social perspectives to political perspectives, whereby mainstream teachers regard national policies and strategies as political responses to demands for inclusion. The findings further showed that teachers’ practices are moving away from the provision of special needs to the promotion of inclusive needs, whereby inclusive education benefits all learners. The study found that some teachers are resisting inclusive education, and would like learners with physical disabilities to return to special schools, thus controverting policy directives. As a way forward, this study recommended that future policy formulation and implementation in the Malawian mainstream secondary education should consider the bioecological systems approach, whereby policymakers systematically interact with the mainstream secondary teachers to improve their perspectives, competencies and practices on inclusive education.
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- Date Issued: 2021
Exploring the influence of marine science camps on learners’ motivation and dispositions towards scientific inquiry
- Authors: Hambaze, Nozipiwo
- Date: 2020
- Subjects: Science projects , Marine sciences -- Study and teaching -- South Africa -- Case studies , Active learning , Science camps -- South Africa -- Case studies , Experiential learning , Science -- Study and teaching -- South Africa -- Case studies , Motivation in education -- South Africa -- Case studies
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/163626 , vital:41062
- Description: For a number of years, it has been observed that learners’ motivation towards science has decreased , especially when th ey get to higher grades in secondary school. Strengthening learners’ motivation has become a very important area for educators to understand and reflect on. Many studies suggest that inquiry - based learning activities can provide a conducive learning enviro nment and build up learners ’ abilities and motivation to learn science beyond the classroom. It is against this backdrop that t his interventionist study s ought to investigate the influence of marine science camps on learners’ motivation towards scientific inquiry. The study was informed by Vygotsky ’s social constructivist theory and Wenger ’s community of practice theory. The participants were 21 grade 10 science learners from seven high schools in the Eastern Cape in South Africa. In order to answer the research questions , data w ere collected through the use of Student Motivation T owards Science Learning (SMTSL) questionnaires prior and post science camp, learner reflection journals and focus group interviews. Excel was used to analyse quantitative data w hereas an inductive - deductive thematic approach was used to analyse the qualitative data.The findin gs of the study revealed that through active participation learners’ dispositions shifted and became more positive after the scientific inquiry activity experience at the marine science camp. Furthermore , the findings of the study revealed that as a result of the marine science camp experiences learners were highly motivated to develop their own marine related science projects for the science fairs. Science fairs bear the testimony as one learner did not only co nduct a marine related project for a science fair but was awarded a gold medal at a regional science fair and a silver medal the national fair. The same lea r ne r was awarded an opportunity to present his project in other international fair. Notably also, l earners displayed increase science motivation as a result of participating at the marine science camp. iv This study thus recommends that i n order to enhance scie ntific inquiry among science learners, efforts in developing out - of - school programs not only by th e department of education but also other stakeholders such as universities are needed to inspire and motivate more learners in sciences. Additionally , pure sc ience faculties and social science s should work closely with each other to promote science.
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- Date Issued: 2020
Aspects of the population ecology, habitat use and behaviour of the endangered Knysna seahorse (Hippocampus capensis Boulenger, 1900) in a residential marina estate, Knysna, South Africa: implications for conservation
- Authors: Claassens, Louw
- Date: 2018
- Subjects: Endangered species -- South Africa -- Knysna , Sea horses -- Behavior -- South Africa -- Knysna , Sea horses -- Habitat -- South Africa -- Knysna , Sea horses -- Ecology -- South Africa -- Knysna
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/54789 , vital:26616
- Description: The Knysna seahorse Hippocampus capensis is South Africa’s only endemic seahorse species, and is found in only three adjacent estuaries along the southern coast. The conservation of this endangered species is important on a national and international level. This study presents the first research on this species within the Knysna estuary since 2001 and specifically focuses on aspects of its ecology within a residential marina estate (Thesen Islands Marina). The physico-chemical and habitat features of the marina were described and the population ecology, habitat use, and behaviour of the Knysna seahorse were investigated. Physico-chemical conditions within the western section of the marina, characterised by high water current velocities, were similar to that of the adjacent estuary. The eastern section of the marina was characterised by lower water current velocities and higher turbidity. Four major habitat types were identified within the marina canals: (I) artificial Reno mattress (wire baskets filled with rocks); (II) Codium tenue beds; (III) mixed vegetation on sediment; and (IV) barren canal floor. Seahorse densities within the marina were significantly higher compared to densities found historically within the estuary. Highest seahorse densities were specifically found within the artificial Reno mattress structures and within the western section of the marina. Seahorse density varied spatially and temporally and the type of habitat was an important predictor for seahorse occurrence. An experimental investigation found that H. capensis chooses artificial Reno mattress habitat over Zostera capensis when given a choice. GoPro cameras were used successfully to investigate daytime seahorse behaviour within the Reno mattress habitat. Seahorses were more active during the morning, spent most of their time (> 80 %) feeding, and morning courting behaviour for this species were confirmed. However, during the summer holiday period (mid-December to mid-January) few seahorses were observed on camera, which suggests that the increase in motor boat activity and the related increase in noise had a negative effect on H. capensis feeding and courting behaviour. The marina development, and in particular the Reno mattresses, created a new habitat for this endangered species within the Knysna estuary. In addition to the protection and restoration of natural habitats in which H. capensis is found, the conservation potential of artificial structures such as Reno mattresses should be realised.
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- Date Issued: 2018
The investigation of type-specific features of the copper coordinating AA9 proteins and their effect on the interaction with crystalline cellulose using molecular dynamics studies
- Authors: Moses, Vuyani
- Date: 2018
- Subjects: Copper proteins , Cellulose , Molecular dynamics , Cellulose -- Biodegradation , Bioinformatics
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/58327 , vital:27230
- Description: AA9 proteins are metallo-enzymes which are crucial for the early stages of cellulose degradation. AA9 proteins have been suggested to cleave glycosidic bonds linking cellulose through the use of their Cu2+ coordinating active site. AA9 proteins possess different regioselectivities depending on the resulting cleavage they form and as result, are grouped accordingly. Type 1 AA9 proteins cleave the C1 carbon of cellulose while Type 2 AA9 proteins cleave the C4 carbon and Type 3 AA9 proteins cleave either C1 or C4 carbons. The steric congestion of the AA9 active site has been proposed to be a contributor to the observed regioselectivity. As such, a bioinformatics characterisation of type-specific sequence and structural features was performed. Initially AA9 protein sequences were obtained from the Pfam database and multiple sequence alignment was performed. The sequences were phylogenetically characterised and sequences were grouped into their respective types and sub-groups were identified. A selection analysis was performed on AA9 LPMO types to determine the selective pressure acting on AA9 protein residues. Motif discovery was then performed to identify conserved sequence motifs in AA9 proteins. Once type-specific sequence features were identified structural mapping was performed to assess possible effects on substrate interaction. Physicochemical property analysis was also performed to assess biochemical differences between AA9 LPMO types. Molecular dynamics (MD) simulations were then employed to dynamically assess the consequences of the discovered type-specific features on AA9-cellulose interaction. Due to the absence of AA9 specific force field parameters MD simulations were not readily applicable. As a result, Potential Energy Surface (PES) scans were performed to evaluate the force field parameters for the AA9 active site using the PM6 semi empirical approach and least squares fitting. A Type 1 AA9 active site was constructed from the crystal structure 4B5Q, encompassing only the Cu2+ coordinating residues, the Cu2+ ion and two water residues. Due to the similarity in AA9 active sites, the Type force field parameters were validated on all three AA9 LPMO types. Two MD simulations for each AA9 LPMO types were conducted using two separate Lennard-Jones parameter sets. Once completed, the MD trajectories were analysed for various features including the RMSD, RMSF, radius of gyration, coordination during simulation, hydrogen bonding, secondary structure conservation and overall protein movement. Force field parameters were successfully evaluated and validated for AA9 proteins. MD simulations of AA9 proteins were able to reveal the presence of unique type-specific binding modes of AA9 active sites to cellulose. These binding modes were characterised by the presence of unique type-specific loops which were present in Type 2 and 3 AA9 proteins but not in Type 1 AA9 proteins. The loops were found to result in steric congestion that affects how the Cu2+ ion interacts with cellulose. As a result, Cu2+ binding to cellulose was observed for Type 1 and not Type 2 and 3 AA9 proteins. In this study force field parameters have been evaluated for the Type 1 active site of AA9 proteins and this parameters were evaluated on all three types and binding. Future work will focus on identifying the nature of the reactive oxygen species and performing QM/MM calculations to elucidate the reactive mechanism of all three AA9 LPMO types.
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- Date Issued: 2018
Transcription factor binding specificity and occupancy : elucidation, modelling and evaluation
- Authors: Kibet, Caleb Kipkurui
- Date: 2017
- Subjects: Transcription factors , Transcription factors -- Data processing , Motif Assessment and Ranking Suite
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:21185 , http://hdl.handle.net/10962/6838
- Description: The major contributions of this thesis are addressing the need for an objective quality evaluation of a transcription factor binding model, demonstrating the value of the tools developed to this end and elucidating how in vitro and in vivo information can be utilized to improve TF binding specificity models. Accurate elucidation of TF binding specificity remains an ongoing challenge in gene regulatory research. Several in vitro and in vivo experimental techniques have been developed followed by a proliferation of algorithms, and ultimately, the binding models. This increase led to a choice problem for the end users: which tools to use, and which is the most accurate model for a given TF? Therefore, the first section of this thesis investigates the motif assessment problem: how scoring functions, choice and processing of benchmark data, and statistics used in evaluation affect motif ranking. This analysis revealed that TF motif quality assessment requires a systematic comparative analysis, and that scoring functions used have a TF-specific effect on motif ranking. These results advised the design of a Motif Assessment and Ranking Suite MARS, supported by PBM and ChIP-seq benchmark data and an extensive collection of PWM motifs. MARS implements consistency, enrichment, and scoring and classification-based motif evaluation algorithms. Transcription factor binding is also influenced and determined by contextual factors: chromatin accessibility, competition or cooperation with other TFs, cell line or condition specificity, binding locality (e.g. proximity to transcription start sites) and the shape of the binding site (DNA-shape). In vitro techniques do not capture such context; therefore, this thesis also combines PBM and DNase-seq data using a comparative k-mer enrichment approach that compares open chromatin with genome-wide prevalence, achieving a modest performance improvement when benchmarked on ChIP-seq data. Finally, since statistical and probabilistic methods cannot capture all the information that determine binding, a machine learning approach (XGBooost) was implemented to investigate how the features contribute to TF specificity and occupancy. This combinatorial approach improves the predictive ability of TF specificity models with the most predictive feature being chromatin accessibility, while the DNA-shape and conservation information all significantly improve on the baseline model of k-mer and DNase data. The results and the tools introduced in this thesis are useful for systematic comparative analysis (via MARS) and a combinatorial approach to modelling TF binding specificity, including appropriate feature engineering practices for machine learning modelling.
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- Date Issued: 2017