Finding Frankenflora: investigating hybridisation between local and introduced Protea species at the Van Stadens Wildflower Reserve
- Authors: Macqueen, Timothy
- Date: 2019
- Subjects: Proteaceae -- South Africa , Proteaceae Trees -- South Africa Hybridization
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/40724 , vital:36230
- Description: This study provides an overview of hybridisation and methods to detect it in plants. This is documented in chapter 1. Another aim was to identify hybridisation between local and non-local species in the Proteaceae. This is covered in chapter 2. Finally the third aim was to develop a cheap and rapidly working method of detecting hybrids in this system. This is detailed in chapter 3. The model system for this research is the Van Stadens Wildflower Reserve. In 1984 the reserve manager introduced the non-local species, Protea susannae from Cape Agulhas. This represents a long-distance dispersal of over 600 km. This study is focused on looking at the impacts of the introduction of P. susannae on the local species P. eximia. I use genetic and morphological methods to detect hybrids. Hybridisation and the methods implemented to identify it are reviewed and discussed (Chapter 1). Morphological traits of the two species and direct sequencing of the nuclear ITS and chloroplast regions are compared (Chapter 2). This includes notes on the detection of hybrids and the potential for cryptic hybrids. I also focussed on testing of two rapid and low-cost techniques for detecting hybrids using Species Specific Primers (SSP’s) and High Resolution Melt (HRM). The Sanger sequencing method and ITS detected cryptic hybrids. The HRM was able to detect hybrids when comparing F1 putative hybrids to a ‘pure’ parent populations, but was not able to detect between a ‘pure’ parent population and F3 or F4 putative hybrids in the Van Stadens system. The SSP’s had the highest hybrid detection rate. Genetic methods, specifically the SSP’s were found to work well to detect hybrids in an environment where a hybrid swarm has taken place. The maps produced from this thesis will be useful for determining the distribution of hybrids in the reserve and for other similar hybrid systems. Long distance dispersal of Cape species may lead to the loss of genetic diversity or species as local and non-local hybridisation may occur, similar to the hybridisation between P. susannae and P. eximia in the Van Stadens Wild Flower Reserve. Genetic methods were shown to be superior to morphological hybrid detection (i.e. Sanger sequencing and SSP’s). The SSP approach tested here, which is dependent on the presence of fixed species specific SNP’s (Single Nucleotide Polymorphisms), could offer a rapid and effective method to explore hybridisation through space and time.
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- Date Issued: 2019
Estimating maize grain yield from crop growth stages using remote sensing and GIS in the Free State Province, South Africa
- Authors: Mditshwa, Sithembele
- Date: 2017
- Subjects: Crop yields Crops -- Physiology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/6016 , vital:29481
- Description: Early yield prediction of a maize crop is important for planning and policy decisions. Many countries, including South Africa use the conventional techniques of data collection for maize crop monitoring and yield estimation which are based on ground-based visits and reports. These methods are subjective, very costly and time consuming. Empirical models have been developed using weather data. These are also associated with a number of problems due to the limited spatial distribution of weather stations. Efforts are being made to improve the accuracy and timeliness of yield prediction methods. With the launching of satellites, satellite data are being used for maize crop monitoring and yield prediction. Many studies have revealed that there is a correlation between remotely sensed data (vegetation indices) and crop yields. The satellite based approaches are less expensive, save time, data acquisition covers large areas and can be used to estimate maize grain yields before harvest. This study applied Landsat 8 satellite based vegetation indices, Normalized Difference Vegetation Index (NDVI), Soil Adjusted Vegetation Index (SAVI) and Moisture Stress Index (MSI) to predict maize crop yield. These vegetation indices were derived at different growth stages. The investigation was carried out in the Kopanong Local Municipality of the Free State Province, South Africa. Ground-based data (actual harvested maize yields) was collected from Department of Agriculture, Forestry and Fisheries (DAFF). Satellite images were acquired from Geoterra Image (Pty) Ltd and weather data was from the South African Weather Service (SAWS). Multilinear regression approaches were used to relate yields to the remotely sensed indices and meteorological data was used during the development of yield estimation models. The results showed that there are significant correlations between remotely sensed vegetation indices and maize grain yield; up to 63 percent maize yield was predicted from vegetation indices. The study also revealed that NDVI and SAVI are better yield predictors at reproductive growth stages of maize and MSI is a better index to estimate maize yield at both vegetative and reproductive growth stages. The results obtained in this study indicated that maize grain yields can be estimated using satellite indices at different maize growth stages.
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- Date Issued: 2017
What limits an invasive biotic and abiotic effects on the distribution of the invasive mussel mytilus galloprovincialis on the South African coastline
- Authors: Hall, Madison
- Date: 2015
- Subjects: Perna , Mytilus galloprovincialis , Mussels -- South Africa , Introduced organisms -- Environmental aspects -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5927 , http://hdl.handle.net/10962/d1017805
- Full Text:
- Date Issued: 2015
Woody encroachment and plant-root soil interactions in a semi-arid savanna
- Authors: Gusha, Bukho
- Date: 2015
- Subjects: Pastures -- Management Weeds Invasive plants
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/12139 , vital:39174
- Description: Savannas occupy 1/8 of the global land surface, support a large proportion of the world’s human population and the majority of its rangeland and livestock. Woody encroachment has been reported as the major challenge in these landscapes. This study describes the differences between three contrasting tree density classes in a semi- arid savanna with the view to developing an improved understanding of woody encroachment which is prevalent in this region. The study attempted to determine if there was a relationship between lateral root distributions at varying soil depths with increasing levels of woody encroachment, and to compare species composition and soil water profiles in these rangelands. Three homogeneous vegetation units, namely: sparsely encroached (HVU1), grassland (HVU2) and Albany thicket (HVU3) were identified for the study sites. A Trench method was used determine root biomass and a step point method was used to determine herbaceous species composition in all the HVUs. The results showed that more Decreaser species (especially Themeda triandra) were recorded in a sparsely encroached site (HVU1) and grassland site (HVU2), while Cynodon dactylon was mostly recorded in the thicket site (HVU3). Acacia karroo was mostly recorded in HVU1 while in HVU2 other woody species such as Coddia rudis and Grewia occidentalis were also recoded. HVU3 had the poorest basal cover (point to tuft distance) (22.60 cm) while HVU1 (9.93 cm) and HVU2 (7.73 cm) had moderate basal cover. The herbaceous standing biomass was significantly different across the HVUs. HVU3 was higher (1206.15 kg ha-1) than HVU1 (942.43 kg ha-1) and HVU2 (677.10 kg ha-1). The soil moisture content was significantly different between the HVUs, but was not significantly different between the depths (p< 0.05). High soil moisture content was recorded in HVU3 compared to other HVUs. The results of Analysis of Variance (ANOVA) showed that soil depth and the type of homogenous vegetation unit (HVU) had significant effects (p<0.01) on root biomass. The pair wise t-test showed that there were no significant difference in root biomass between sparsely encroached (HVU1) and grassland (HVU2) sites (p>0.05), but there were significant differences in root biomass between grassland (HVU2) and thicket (HVU3) site (p<0.05). The mean for total root biomass found in the study was 2.66 kg m-2. In all the trenches most of the root biomass was found in depth 1 (0-30 cm) which was 2.43 kg m-2followed by 1.32 kg m-2 in depth 2 (30-60 cm) and 0.49 kg m-2 in depth 3 (60-90 cm). According to the results on species composition, herbaceous biomass, basal cover, soil moisture content and the root biomass, Kwezana communal rangeland has a potential of running a sustainable livestock production enterprise if proper management practices can be implemented. To improve the rangelands of Kwezana communal rangelands, management such as proper resting, burning, proper stocking rates and physically clearing of bushes should be considered.
- Full Text:
- Date Issued: 2015
Woody encroachment and plant-root soil interactions in a semi-arid savanna
- Authors: Gusha, Bukho
- Date: 2015
- Subjects: Pastures -- Management Weeds Invasive plants
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/12053 , vital:39131
- Description: Savannas occupy 1/8 of the global land surface, support a large proportion of the world’s human population and the majority of its rangeland and livestock. Woody encroachment has been reported as the major challenge in these landscapes. This study describes the differences between three contrasting tree density classes in a semi- arid savanna with the view to developing an improved understanding of woody encroachment which is prevalent in this region. The study attempted to determine if there was a relationship between lateral root distributions at varying soil depths with increasing levels of woody encroachment, and to compare species composition and soil water profiles in these rangelands. Three homogeneous vegetation units, namely: sparsely encroached (HVU1), grassland (HVU2) and Albany thicket (HVU3) were identified for the study sites. A Trench method was used determine root biomass and a step point method was used to determine herbaceousspecies composition in all the HVUs. The results showed that more Decreaser species (especially Themeda triandra) were recorded in a sparsely encroached site (HVU1) and grassland site (HVU2), while Cynodon dactylon was mostly recorded in the thicket site (HVU3). Acacia karroo was mostly recorded in HVU1 while in HVU2 other woody species such as Coddia rudis and Grewia occidentalis were also recoded. HVU3 had the poorest basal cover (point to tuft distance) (22.60 cm) while HVU1 (9.93 cm) and HVU2 (7.73 cm) had moderate basal cover. The herbaceous standing biomass was significantly different across the HVUs. HVU3 was higher (1206.15 kg ha-1) than HVU1 (942.43 kg ha-1) and HVU2 (677.10 kg ha-1). The soil moisture content was significantly different between the HVUs, but was not significantly different between the depths (p< 0.05). High soil moisture content was recorded in HVU3 compared to other HVUs. The results of Analysis of Variance (ANOVA) showed that soil depth and the type of homogenous vegetation unit (HVU) had significant effects (p<0.01) on root biomass. The pair wise t-test showed that there were no significant difference in root biomass between sparsely encroached (HVU1) and grassland (HVU2) sites (p>0.05), but there were significant differences in root biomass between grassland (HVU2) and thicket (HVU3) site (p<0.05). The mean for total root biomass found in the study was 2.66 kg m-2. In all the trenches most of the root biomass was found in depth 1 (0-30 cm) which was 2.43 kg m-2followed by 1.32 kg m-2 in depth 2 (30-60 cm) and 0.49 kg m-2 in depth 3 (60-90 cm). According to the results on species composition, herbaceous biomass, basal cover, soil moisture content and the root biomass, Kwezana communal rangeland has a potential of running a sustainable livestock production enterprise if proper management practices can be implemented. To improve the rangelands of Kwezana communal rangelands management such as proper resting, burning, proper stocking rates and physically clearing of bushes should be considered.
- Full Text:
- Date Issued: 2015
Water quality, abalone growth and the potential for integrated mariculture on a South African abalone Haliotis midae L. farm
- Authors: Yearsley, Rowan David
- Date: 2008
- Subjects: Abalones -- South Africa Abalone culture -- South Africa Abalones -- Growth Mariculture -- South Africa Water quality -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5334 , http://hdl.handle.net/10962/d1005180
- Description: Abalone Haliotis midae farming in South Africa is highly intensive, employing pump-ashore, flow-through systems. Despite the known sensitivity of abalone to water quality, there is only a rudimentary understanding of water quality dynamics on South African abalone farms and its effects on abalone production. Furthermore, the potential for reusing the relatively dilute abalone farm effluent to culture other animal species has not been investigated. This study investigated the dynamics of water quality and growth on a South African abalone farm and assessed the suitability of the effluent for the culture of silver kob Argyrosomus inodorus and bloodworm Arenicola loveni loveni. Monitoring of water quality and abalone growth in abalone tanks revealed that oxygen concentrations decreased, while H⁺ ion and free-ammonia (NH₃) concentration increased in a gradient between the inflow and outflow. Abalone growth was positively correlated with oxygen concentration and negatively correlated with free-ammonia and H⁺ ion concentration. The oxygen (O) concentration of the farm influent was dependent upon the influents’ temperature (T) and was described by the relationship O (mg L⁻¹) = 11.244 – 0.208T (r²=0.74). Linear regression analysis of data collected from abalone farm tanks revealed that the concentration of total ammonia at the outflow of abalone tanks (μg TAN L⁻¹) was dependant upon temperature (°C), flow-rate (L s⁻¹ kg⁻¹ H. midae), abalone size (g) and length of time since the tank was last cleaned (d) (n = 125, r² = 0.80). The production of total ammonia (μg TAN s⁻¹ kg⁻¹) was related to temperature, abalone size and days that the tanks remained un-cleaned (n = 125; r² = 0.81). A diurnal cycle of respiration was evident in abalone tanks with higher oxygen consumption and H+ ion production at night. The oxygen concentration of farm effluent was related to temperature, farm biomass and flow rate by means of a linear regression equation (n = 40; r² = 0.69). The results demonstrated the importance of optimising the flow-rate per unit of biomass for various temperatures and sizes of abalone. As abalone size and temperature cannot be controlled under farm conditions, the flow-rate per unit of biomass which the abalone culture system receives will determine the quality of the culture water. The specific growth rate (0.48 ± 0.01 % BW d⁻¹), mortality (1.8 ± 0.5 %), feed conversion ratio (3.0 ± 0.2) and protein efficiency ratio (1.0 ± 0.1) of silver kob kept in either abalone farm effluent or control seawater for 120 days did not differ significantly (t-test, P>0.05). A 90 day growth trial indicated that abalone farm effluent is a suitable culture medium for bloodworm. Bloodworm supplied with control seawater lost weight at 0.19 ± 0.04 % BW d-1, while those given abalone effluent grew at 0.39 ± 0.07 % BW d⁻¹. Mortality was 6 ± 3 % in effluent and 11 ± 8 % in seawater. The bloodworm were efficient at processing solid waste. Abalone farm effluent initially contained 7.7 ± 13 mg L⁻¹ more suspended solids than control seawater, which contained 3.5 ± 0.5 mg L⁻¹, but after passing through bloodworm systems the concentration in abalone effluent was reduced to only 1.4 ± 3.5 mg L⁻¹ above that in control seawater. Therefore, abalone farm effluent could be reused as a culture medium for both silver kob and bloodworm. Future work is needed to investigate aspects of the feasibility of such systems such as growth rates at different sizes and stocking densities.
- Full Text:
- Date Issued: 2008
Characterisation of InAs-based epilayers by FTIR spectroscopy
- Authors: Baisitse, Tshepiso Revonia
- Date: 2007
- Subjects: Fourier transform infrared spectroscopy , Gallium arsenide semiconductors
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10534 , http://hdl.handle.net/10948/474 , Fourier transform infrared spectroscopy , Gallium arsenide semiconductors
- Description: This study focuses on the characterization of InAs and InAs1-xSbx epitaxial layers by infrared reflectance and transmittance spectroscopy and Hall measurements. Reflectance measurements were performed in order to obtain the dielectric parameters and to extract from these information about the electrical properties (mobility and carrier concentration) of this important III-V material system. The transmittance measurements were used to determine the bandgap of InAsSb. Infrared reflectivity and transmittance measurements were performed in the wavelength range 200 – 2000 cm-1 on InAs and InAsSb layers grown on three types of substrates. A classical two oscillator model that takes into account both the free carriers and the lattice, was used to analyse the reflectance data using the BMDP® computer curve fitting software. The dielectric parameters and the electrical properties (carrier concentration and mobility) were extracted from the simulations. Due to the low free carrier concentration in the epitaxial structures, the plasma resonance frequency (ωp) values obtained from the simulations of reflectance spectra measured above 200 cm-1, were in the order of 20-30 cm-1. These low values were confirmed by direct measurements of ωp in reflectance spectra obtained in the range 15-200 cm-1. The simulated carrier concentration and mobility values determined optically were compared to the values determined by Hall measurements at room temperature and previously reported values by other researchers. The simulated values obtained were in reasonable agreement with the Hall values. The simulated and measured carrier concentrations obtained for InAs layers were significantly higher than the intrinsic carrier concentration for InAs at room temperature, indicating notable concentrations of donors resulting from the growth process.
- Full Text:
- Date Issued: 2007
Separation and characterisation of chromium (III) carboxylate solutions
- Authors: Bryson, Michael Andrew Walker
- Date: 1981
- Subjects: Chromium
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4416 , http://hdl.handle.net/10962/d1006847
- Description: From Summary: The complexes present in chromium(III) carboxylate solutions (acetate or formate) were separated using ion exchange chromatography, electrophoresis and gel filtration. Eight cationic species and one non-ionic species with charges from 0 to +3 and nuclearities ranging from mononuclear to quadranuclear were isolated from chromium(III) acetate solutions. Six cationic species with charges from +1 to +3 and nuclearities ranging from mononuclear to trinuclear were isolated from chromium(III) formate solutions. The empirical formulae for these complexes were determined by analytical methods. Infra- red spectroscopy was used to distinguish between monodentate and bidentate bridged carboxylate ligands. An immediate change in the visible spectrum of a complex on its acidification was used as evidence that a terminal hydroxo group was present.
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- Date Issued: 1981