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An in silico analysis, purification and partial kinetic characterisation of a serine protease from Gelidium pristoides

  • Authors: Ntsata, Zolani
  • Date: 2020
  • Subjects: Gelidium Proteolytic enzymes
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10353/12076 , vital:39149
  • Description: The aim of this study was to characterize the protease enzyme (s) from red algae. An in silico analysis of red algae genomes was used to identify gene coding for protease. Protease sequences identified from these genomes were examined for conserved domains, active site and structures. The domain search revealed that the identified sequences were from the five classes of protease enzymes. For function inference, the red algae sequences were aligned to identify the catalytic sites, and the tertiary structures were predicted using homology modelling. An in silico analysis provides an indication of the class and potential functions of the enzymes. However, it cannot predict whether the gene is constitutively expressed in the red algae or under which conditions it may be induced, and it cannot determine the kinetic efficiency of an enzyme against various substrate, or the optimum conditions for the protein activity. Attempts to clone and recombinantly express selected red algae proteases, proved unsuccessful, as the available genomes where from red algae species found mainly in Asia, and the designed primers, therefore, did not amplify a corresponding PCR product from the red algae harvested in South Africa. Crude extracts of red algae collected from Kenton-on-Sea, along the East Coast of South Africa, were screened for protease activity using Benzoyl-Arginine-pNitroAnilide (BApNA) as substrate. The proteases detected in the crude extract were purified using ammonium sulphate precipitation and HiPrep DEAE FF 16/10; CM FF 16/10, and HiPrep Q FF 16/10 columns for ion-exchange chromatography. The HiPrep Q FF 16/10 column yielded active protein, which revealed two bands of 11kDa and 17kDa on SDS-PAGE. It was assumed that these bands represented two subunits of the purified protease. Kinetic characterisation of the purified protease revealed a pH optimum of 9, using BApNA as substrate, a temperature optimum at 60ºC, and sensitivity to temperature when stored above 4ºC. The protease activity was inhibited by Ferric chloride (32%), induced by calcium chloride (156%), no inhibition by magnesium chloride (97%) and slight inhibition by potassium chloride (77%) and manganese chloride (70%). Phenylmethylsulfonyl fluoride (PMSF), a serine protease inhibitor, almost totally inhibited the protease activity, indicating that the protease from red algae was most likely a serine protease. The Km and kcat values were 1.96 µM, and 0.364 s -1 , respectively using BApNA as the substrate. This study revealed that the red algae genome contains numerous genes that encode for proteases from almost all the classes of proteases. A serine protease from the red algae Gelidium pristoides was partially purified and kinetically characterised, confirming that red algae found along the Eastern Coast of South Africa contain genes that express active proteases that may be of medical or industrial interest. Further studies, however, are required to recombinantly express, purify and characterise the numerous proteases encoded by the genes identified in the in silico analysis of this study.
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  • Date Issued: 2020

Nostalgia in reimagining the past: the subjectivity of memory in the representation of history. A textual analysis of Rehad Desai's documentary films

  • Authors: Dlamini, Philani Vincent
  • Date: 2019
  • Subjects: Documentary films -- South Africa , Nostalgia in motion pictures Nostalgia in mass media
  • Language: English
  • Type: Thesis , Masters , MA
  • Identifier: http://hdl.handle.net/10948/45183 , vital:38261
  • Description: South Africa occupies a unique space in terms of the decolonization of the continent of Africa. While massive projects of decolonization where happening across the continent, South Africa was subjected to a conservative and racialised project of segregation. This arrested development makes for an interesting anachronism in South Africa as disconcerting “Third- World” and “First-World” economies emerged creating an anomalous temporality. I was born just a month before the inimitable Ruth First was unceremoniously assassinated in Mozambique in 1982. While further South, one of the most underreported conflicts of apartheid South Africa was in its nascent stages no further than a kilometer away from my house. I am referring of course to the violent clashes between factions of both the Inkatha Freedom Party (IFP) and the African National Congress (ANC), played in the men’s hostel of the third largest township in the country, Umlazi, South of Durban. These would only come to have meaning to me later, when I was at university. I mention these cultural and perhaps socio-political artefacts in so far as they relate to the kind of environment that lead to this research enquiry. Which is to say that as social beings, we are in fact products of the things that affect the social environment that we exist in. This is not a new idea. What is particularly interesting for this enquiry is the eclecticism of the emblems that survive to shapes one’s own identity and perception of the world around them. Within the above stated mini-biography lies a complex matrix of emotions and extrapolated meanings mediated through a conflicted and negotiated understanding of what the social history of South Africa meant for my own personal history. This paper is an attempt to think through articulations of time as they are constituted by future-orientated subjectivities extending back to varied pasts. It does so by exploring a recent work of black South African self-writing, Jacob Dlamini’s Native Nostalgia (2009). Considering the text’s treatment of time, I argue that porous conceptions of temporality open up possibilities for self-enunciation. What Paul Gilroy has described as “the signs of sameness” (2000, 101). Meaning that these could be quantified and as such researchable and in fact applied across various cultural texts (including but not limited to film). The body of work from South African documentary filmmaker Rehad Desai provides an interesting case study to examine Jacob Dlamini’s thematic pre-occupations with nostalgia. Nostalgia here is used to see if such pre-occupations can be applied to a filmic body of work. Desai’s body of work is interesting to this enquiry because it almost exclusively deals with the temporalities of South Africa as “refracted through the prism of the past”. By this I mean Desai through his work appears to reflect on South Africa’s storied past as it affects current happenings. It is the intention of this paper to argue that Desai deals with his subject (the evolution of the South African political landscape) in similar terms to the way Jacob Dlamini explores the notion of reflective nostalgia.
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  • Date Issued: 2019

An intervention for high-risk type 2 diabetic clients during preconception and internatal in re-engineering of primary health care

  • Authors: Ngoma, Siphokazi Amanda
  • Date: 2018
  • Subjects: Diabetes Diabetes Mellitus, Type 2
  • Language: English
  • Type: Thesis , Masters , (MCur) Nursing
  • Identifier: http://hdl.handle.net/10353/10199 , vital:35375
  • Description: The South African disease profile has changed significantly and has increasing prevalence of overweight and obesity in relation to body mass index. Type 2 diabetes mellitus is greatly associated with increased body mass index and poses a great health concern for women of childbearing age. Complications of type 2 diabetes mellitus in pregnancy has life-threatening for the mother and baby. South African women have a tendency of avoiding and neglecting routine screening before pregnancy, that is part of preconception care or internatal care. The aim of the study sought to identify women of childbearing age with predisposing factors of type 2 diabetes, in order to intervene, using a lifestyle modification to delay and prevent the occurrence of type 2 diabetes. A quantitative intervention study using parallel group randomized control trials was used. One hundred and forty-six women of childbearing age (18-45 years) were recruited and assigned to either control (n=73 + 9) or experimental group (n=73 +9). using a computer software randomizer application to avoid bias in selection of participants by the researcher. Both groups received health education from the researcher and the dietician from hospital. The control group had no intervention done, whereas the experimental group attended physical activity sessions which were administered by a hired lay coach. The findings showed that both groups are at risk of developing type 2 diabetes although they both groups lose weight. The weight loss of the control group was owed to education during recruitment. Vegetable consumption was minimal to the younger age group than the older age groups. Waist circumference above 105cm consumed less vegetables, a higher waist circumference is associated with insulin resistance. There was also minimal exercises and knowledge about the preconception care. Diabetes prevention is vital for the women of childbearing age as it affects both mother and child. The study recommends lifestyle modification (exercise and healthy diet) to prevent and delay of type 2 diabetes and gestational diabetes mellitus. The study also recommends Preconception care and Re- engineering of Primary health care uses community health care workers for screening high risk women during home visits.
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  • Date Issued: 2018

The contribution of small scale livestock farming to rural household income in the King William's Town area

  • Authors: Mbangcolo, Nomalungisa
  • Date: 2018
  • Subjects: Farms, Small Livestock farms Rural poor
  • Language: English
  • Type: Thesis , Masters , MAgric
  • Identifier: http://hdl.handle.net/10353/13018 , vital:39432
  • Description: The Eastern Cape Province have high numbers of livestock but high poverty rates remain very high. This study therefore, aimed to determine the contribution of smallscale livestock farming to rural household income in the King William’s Town area in five rural areas. Literature shows that the livestock sector contributes 40 percent of global value of agricultural output and supports the livelihoods and food security of almost a billion people. The data collected for this research were both quantitative and qualitative and cross-section design was employed for the research. The study used both simple random sampling and availability sampling to select 125 sample households, 25 respondents from each village. Descriptive statistics was used in the analysis of demographic and socio economic characteristics of households where graphs and tables were used to present the relevant information. Probit regression model was used to determine factors influencing the decision to practice livestock production and marginal analysis was calculated to determine the effect of changes of individual variables. For the assessment of the contribution of small-scale livestock farming to household income Propensity Score Matching (PSM) model was fitted. Results show that the highest percentage of livestock kept by households in 5 villages is 56 percent for poultry and the lowest percentage is 2 percent for pig. The main source of income for many households in the study area was the income from pensions and grants but livestock farming is also contributing to household’s income through income generated from sales of livestock for customary events such as traditional rituals and celebrations, lobola, funerals and weddings. Many of the households have secondary education and the reason might simply be that in olden days’ parents had no money to pay school fees of their children and that limited children to go further with their studies. The results of the marginal effects estimates for the probit model suggest that changes in the number of livestock sold would have important effects on household incomes. There are various challenges that livestock producers are facing in their production and the major challenge that households are facing is parasites and diseases as they decrease number of livestock kept by households. Parasites and diseases suck blood, causing the animals to become weak and sometimes resulting in high mortality rate. It is recommended that livestock producers should be made aware of the impacts of various challenges that affect livestock farming through awareness creation programmes and suggest solutions to overcome those challenges.
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  • Date Issued: 2018

'n Ondersoek na die uitbeelding van Khoisan-karakters deur wit Afrikaanse prosateurs: 1994-2014

  • Authors: December, Peter
  • Date: 2017
  • Subjects: Khoikhoi (African people) -- South Africa -- History Khoikhoi (African people) -- Race identity -- South Africa
  • Language: English
  • Type: Thesis , Masters , MA
  • Identifier: http://hdl.handle.net/10948/22070 , vital:29819
  • Description: This dissertation offers a literary-thematic investigation based on a postcolonial approach to the representation of the Khoisan and their descendants. I restricted my scope to selected Afrikaans novels at the centre of critical attention between between 1994 and 2014. Earlier novels in this period under discussion are Dolf van Niekerk’s Koms van die hyreën (1994), Willem Kotze’s Tsats van die Kalahari (1994), Die spoorsnyer (1994), Olifantjagters (1997) and Gif (2001) by Piet van Rooyen, plus Karel Schoeman’s Verkenning (1996). Later texts in the focus are Duiwelskloof (1998) and Bidsprinkaan (2005) by André P. Brink, Dalene Matthee’s Pieternella van die Kaap (2000), Eben Venter’s Santa Gamka (2009) and most recently, the Hertzog prize winner of 2015, Buys by Willem Anker (2014). Themes central to South African literature will form the focus of the research, namely intercultural interaction between the first inhabitants of South Africa and missionaries, the question of land ownership, the language motif, and the role of religion (indigenous versus Western belief systems). Attention will also be on more specific issues such as the nature of the relationship between the Khoisan and the colonisers, the characterization of the Khoisan by the selected white authors, as well as other contemporary debates. The secondary objective of the study is to review the historical presence of the Khoisan and their descendants as reflected through the fictional lense of these authors writing over the last two decades, since democratization of the regime in 1994. My focus is particularly on the substantial cultural contribution of the Khoi and the San, as reflected through their representation in fictional works. The question will be posed whether the portrayal of Khoisan characters in novels after 1994 is different from the portrayal in fiction before 1994? My hypothesis is that in the fictional representation one finds a move towards restoration of their human dignity, yet the fact remains that all the authors are white. A different study of fictional works by coloured writers (whose numbers as Afrikaans authors grew substantially after 1994), investigating their representation of the descendants of the Khoi and the San, would in all probability yield radically different results, as the white authors imagine the characters and their consciousness from outside the community and the racial group, whereas the coloured writers belong to the community and the group that they portray.
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  • Date Issued: 2017

An investigation of a Systemic Functional Linguistic approach for teaching Energy to grade 7 Natural Science and Health Education Learners: a Namibian case study

  • Authors: Silvanus, Secilia Tulikefo
  • Date: 2017
  • Language: English
  • Type: Thesis , Masters , MEd
  • Identifier: http://hdl.handle.net/10962/8165 , vital:21361
  • Description: Learners’ general poor performance in science is a concern in science education. The literature mentions pedagogic strategies such as the use of practical activities and inclusion of indigenous knowledge, which are now acknowledged in various science curricula. In addition, many science educators and researchers are exploring innovative pedagogical approaches which may possibly help learners understand science better in order to improve their performance in the subject. Learners’ poor performance in science and possible strategies for improving this, are also a concern in Namibia. The Namibian national examination results (MoE, 2010-2014) revealed that the topic of Energy is one of those in which learners perform poorly. Energy is an important scientific concept that is widely used in various disciplines and it is thus problematic when science learners struggle to make sense of the scientific description of energy and related concepts. This triggered my interest to conduct a study focusing on the topic of energy. The study involved functional recasting from Systemic Functional Linguistic theory, as an intervention during my teaching the topic of energy to English second language grade 7 Natural Science and Health Education learners. The influence of the approach was investigated through the lens of social constructivism. The interpretive paradigm was adopted in order to make meaning of the learners’ experiences, during and after the intervention. Data collection instruments such as pre and post test, stimulated recall interviews, observation and learners’ journals were used. A qualitative approach was used to analyze the data. The pretest and post test results showed that a noticeable shift had occurred in learners’ understanding - the functional recasting teaching approach positively influenced learners’ sense making of energy concepts. Sense-making of energy concepts was evident in learners’ demonstrating the ability to construct and deconstruct technicality, making comprehensible output, meaningful use of gestures, scientific reasoning and asking relevant questions. The results also revealed dialogic discourse and positive attitudes as factors enabling learners’ progress. The language of learning and teaching, and negative attitudes were found to be constraining factors for learners’ progress. Functional recasting might be useful to science teachers and science teacher training programs as the finding for this study revealed that it enabled learners to make sense of energy concepts using scientific English. When using functional recasting, science teachers should consider the constraining factors and possibly avoid or minimize the impact.
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  • Date Issued: 2017

Impact of business confidence on private investments in South Africa

  • Authors: Madzivire, Venna Wadzanayi
  • Date: 2017
  • Subjects: Success in business Confidence Investments
  • Language: English
  • Type: Thesis , Masters , MCom
  • Identifier: http://hdl.handle.net/10353/8910 , vital:33955
  • Description: A wide range of theoretical and empirical studies have been carried out on various determinants of private investments in different economies. This dissertation specifically focuses on the impact business confidence on private investments with regards to the South African economy for the period between 1990 and 2014. The private investment sector in South Africa has, to a greater extent contributed to the overall GDP of the economy. Even though business confidence has a considerable impact on private investments, this study also took into consideration other variables that affect the private investment sector such as interest rates, exchange range and GDP To determine short-run and long-run relationships of business confidence on private Investments in South Africa, a vector error correction model was employed. In order to avoid spurious regression, the ADF test and the PP test were used to test for stationarity. Results of the study indicate that private investments are subject to permanent changes because of changes in business confidence. In addition to that, not all variables have a long-term relationship with private investments but business confidence has a significant long run relationship with private investment. After conducting an econometric analysis, results revealed that Business Confidence and Gross Domestic Product have a positive impact on private investments. On the other hand, interest rates and exchange rates have a negative impact on private investments in South Africa. Various policy recommendations were established on both Private Investments and Business Confidence.
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  • Date Issued: 2017

Pentecostal and exclusion of women in positions of leadership : a case of Pentecostal churches in Harare, Zimbabwe

  • Authors: Guti, Joseph
  • Date: 2017
  • Subjects: Pentecostal churches -- Zimbabwe Women and religion -- Zimbabwe Women in church work
  • Language: English
  • Type: Thesis , Masters , MTh
  • Identifier: http://hdl.handle.net/10353/8866 , vital:33692
  • Description: The primary objective of this study was to investigate the issues relating to women empowerment and their leadership positions in the Pentecostal churches in Harare, Zimbabwe. The study was motivated by the theoretical findings that women have always been discriminated against, be it, politically, economically, socio‐culturally, and also religiously. It is against this background that this study interrogated the position of women in the Church particularly in African Pentecostal Christianity. Scholars of gender studies assert that even though women are attaining higher levels and developing both economically and politically, they still remain invisible in top church leadership ranks. The study examined how compliant the Pentecostals in Zimbabwe are to the gospel of equality, and check if distinctions still exist even among the Pentecostal Christians. This issue of gender imbalance is a worldwide problem which the church needs to address for the good of humanity. The issue of women empowerment in Pentecostal churches particularly in Zimbabwe, is a major challenge today because there are more women than men in the churches, yet it seems that in most churches they are excluded in top leadership positions. The Pentecostal Movement is said to be the most significant religious movement in the world which is primarily made up of women, yet it has also been observed that women in most African countries are treated as lower or second class citizens. The Zimbabwean society is male dominated and the Pentecostal churches are also affected, such that gender imbalances are observed and women abuses are prevalent. Therefore the issue of gender and gender imbalance is a global challenge, and as a result it has also become a global agenda in the academic world. This is what motivated the researcher to examine to what extent the Pentecostal churches in Zimbabwe perceive the importance of women empowerment, and also determine the impact of the exclusion of women from top church leadership positions. A review of literature on women empowerment and their exclusion from Pentecostal church leadership positions indicated that the challenges that Pentecostal women faced were similar among all religious groups in other African countries outside Zimbabwe. The research study showed that the Pentecostal Church in Zimbabwe needs to address this issue of women empowerment since the church or religious sector is lagging behind when other areas of the society like the economic and political sectors have already embraced the notion. Therefore this study is significant because it seeks to address the gender imbalances in Pentecostal Christianity that has seen women being on the periphery while men being at the centre when it comes to Church hierarchy. In investigating the issue of women empowerment in Pentecostal churches the researcher proposes that if there is gender balance in the leadership of the church there will be more sound contribution in the ministry of the church that would benefit everyone equally. It has been observed that women like men, have also been given spiritual gifts that should be used to benefit or minister to the body of Christ without limits. Yet currently there seems to be a sense that women in the Pentecostal Churches are not afforded opportunity to exercise their ministry and freely participate in the active ministry of the church. There is also a general feeling among women that their needs are not adequately catered for in the church because men cannot fully understand the needs of women, and as a result women suffer silently. Iidentification of the above challenges would enable the Pentecostal church leaders and other stakeholders to devise specific policies and strategies that assist in empowering women, and thereby creating a better environment which allows for church growth. This research was a descriptive case study that used self-administered questionnaires and interviews as the primary source of data on a target sample of 80 Pentecostal members (among them were pastors, elders, deacons, and ordinary male and female members) from various Pentecostal churches in Harare. The researcher also consulted secondary sources of data for the study including electronic and printed church material, websites, conferences and church services. Data collected for this research was transformed for statistical analysis through the use of Statistical Packages for Social Sciences (SPSS) software and also Thematic Analysis for further data presentation. Thematic analysis method was used to systematically find answers to research questions, as well as identifying, analyzing and reporting patterns or themes within the data collected. The phenomenological approach was used in this research to understand the behavioural patterns of Pentecostal church members from the view point of the participants, and mostly to allow the voices of the women to be heard. Besides the phenomenological approach, this study employed the Theological Reflection approach to interpret the data since the concerns under investigation are ethical-theological issues. Conclusively, the research findings indicated that women in the Pentecostal churches faced challenges, even though they had capacity just like men. Some of the challenges were due to such factors as the “glass ceiling” that block their access to top leadership ranks, gender role stereotyping, negative societal or cultural influences, inferiority complex of women themselves, lack of acceptance by men, lack of education or access to training, and the challenges of balancing home and family responsibilities. From the study it shows that women have capacity for leadership in Pentecostal churches if they are empowered. Therefore motivational teachings and training programs are needed to correct the wrong mentalities concerning the status of women in African Pentecostalism.
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  • Date Issued: 2017

Screening of shark liver extracts for potential antimicrobial properties against selected pathogenic bacterial strains

  • Authors: Mrwetyana, Thandolwethu
  • Date: 2017
  • Subjects: Fishes -- Diseases Pathogenic bacteria Antibiotics
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10353/12982 , vital:39413
  • Description: The growing problem of antimicrobial resistance prevents the effective treatment of bacterial infections. Traditional antibiotics such as penicillin have been rendered ineffective against most microbial pathogens. This has led to an increased need for the development of new and improved drugs. The marine environment contains a great array of organisms with unique biological properties, but still remains one of our most underutilized biological resources. The aim of this study was to screen different shark liver extracts for antimicrobial properties. After optimizing the extraction methods, the liver extracts (oil and aminosterol) of three different shark species, namely the Dogfish (Squalus acanthias), the Catshark (Scyliorhinus capensis) and the Hammerhead (Sphyrna zygaena) shark, were screened for antimicrobial properties using the Kirby-Bauer disc diffusion method against selected bacterial pathogens (Helicobacter pylori, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus cereus), after which the MIC was determined using the modified broth micro-dilution described by Vollekova et al. (2001). The most active extract was fractionated using thin layer chromatography, and TLC-direct bioautography was used to determine the antimicrobial properties of the fractionated compounds. The Folch et al and the Shinnar et al methods yielded the highest extract volumes for oil and aminosterol consecutively, and the catshark and dogfish aminosterol extract showed greater levels of bioactivity against all selected bacterial pathogens, with S. aureus showing highest susceptibility levels to both extracts. A total of 22 compounds were observed in the developed plates with two compounds (Rf 0.53 and 0.79) showing antimicrobial activity. Certain shark liver extracts possess antimicrobial properties that have the potential to be used in the development of new antimicrobial drugs.
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  • Date Issued: 2017

Internal fingerprint extraction

  • Authors: Darlow, Luke Nicholas
  • Date: 2016
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10962/2959 , vital:20347
  • Description: Fingerprints are a non-invasive biometric that possess significant advantages. However, they are subject to surface erosion and damage; distortion upon scanning; and are vulnerable to fingerprint spoofing. The internal fingerprint exists as the undulations of the papillary junction - an intermediary layer of skin - and provides a solution to these disadvantages. Optical coherence tomography is used to capture the internal fingerprint. A depth profile of the papillary junction throughout the OCT scans is first constructed using fuzzy c-means clustering and a fine-tuning procedure. This information is then used to define localised regions over which to average pixels for the resultant internal fingerprint. When compared to a ground-truth internal fingerprint zone, the internal fingerprint zone detected automatically is within the measured bounds of human error. With a mean- squared-error of 21.3 and structural similarity of 96.4%, the internal fingerprint zone was successfully found and described. The extracted fingerprints exceed their surface counterparts with respect to orientation certainty and NFIQ scores (both of which are respected fingerprint quality assessment criteria). Internal to surface fingerprint correspondence and internal fingerprint cross correspondence were also measured. A larger scanned region is shown to be advantageous as internal fingerprints extracted from these scans have good surface correspondence (75% had at least one true match with a surface counterpart). It is also evidenced that internal fingerprints can constitute a fingerprint database. 96% of the internal fingerprints extracted had at least one corresponding match with another internal fingerprint. When compared to surface fingerprints cropped to match the internal fingerprints’ representative area and locality, the internal fingerprints outperformed these cropped surface counterparts. The internal fingerprint is an attractive biometric solution. This research develops a novel approach to extracting the internal fingerprint and is an asset to the further development of technologies surrounding fingerprint extraction from OCT scans. No earlier work has extracted or tested the internal fingerprint to the degree that this research has.
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  • Date Issued: 2016

The relationship between emotional intelligence and success in the project management

  • Authors: Tikwayo, Victor Vuyile
  • Date: 2016
  • Subjects: Project Management--South Africa Emotional Intelligence--South Africa , Tire industry--South Africa
  • Language: English
  • Type: Thesis , Masters , MBA
  • Identifier: http://hdl.handle.net/10948/45300 , vital:38568
  • Description: Project management plays an important role as a common method of reinforcing chosen organisational strategies that leads to sustainable competitive advantage (Steyn, et al. 2013:5). Organisations that respond quickly to customer demands with the use of projects have more opportunities of adding value to their shareholders and increase their market share or responding faster to mitigate risk. A major challenge facing management in many manufacturing organisations is the increasing gap between big investment demands on one hand and the knowledge and expertise on the other hand of the teams that need to make the project a reality. The increasing recognition of failures encountered in projects to meet the expectations of their stakeholders are cited to be jeopardizing the economic development and growth many developing countries like South Africa. As such, organisations are forced to become more resourceful to reduce project failures in an effort to ensure that the positive project outcomes are realized. Relationship management has been identified as one of the aspects that can help improve coordination and communication between all parties involved in a project. As such, emotional intelligence provides a framework for interpersonal skills among project teams, which is claimed to contribute to project success (Clarke, 2010a:14; Davis, 2011:39)The contemporary study, therefore explored the relationship between emotional intelligence (EI) and success in project management. The study was conducted within the context of manufacturing projects in Continental Tyre South Africa (CTSA). Based on the main findings, it was concluded that emotional intelligence plays an important role in project success. Various recommendations from the results of the empirical study are presented in the final chapter.
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  • Date Issued: 2016

The urban housing crisis in Zimbambwe :a case of city of Harare

  • Authors: Mhakakora, Tafadza Clemence
  • Date: 2016
  • Subjects: Housing -- Zimbabwe Housing development -- Zimbabwe Community development -- Zimbabwe
  • Language: English
  • Type: Thesis , Masters , Degree
  • Identifier: http://hdl.handle.net/10353/5148 , vital:29091
  • Description: The issue of human settlements has recently gained much momentum particularly in cities across the world due to rapid rates of urbanization. The housing crisis is manifesting mostly in the cities of the developing countries; the urban poor population is left with no option, they are continuously living in substandard and unsustainable housing conditions due to the desperate housing need. There is a growing trend of migration into urban centers in the developing countries as well as natural population increase in the cities. The inevitable development is the rapid growth of urbanization. The theories on urbanization suggest that the responsible government and local council authorities must be prepared to address socio-economic issues such as the provision of formal housing, infrastructure development and employment creation. The governments in developing countries are struggling to balance economic development and the provision of social services. As a result, the human settlement sector is suffering lack of prioritization when it comes to budget and resource allocation. The resultant factors are the overcrowding of the urban population, high housing backlogs and dilapidation of infrastructure visible mostly in the major cities of the developing countries.
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  • Date Issued: 2016

"To learn how to speak": a study of Jeremy Cronin's poetry

  • Authors: Pinnock, William
  • Date: 2014
  • Subjects: Poets, South African , Historical materialism
  • Language: English
  • Type: Thesis , Masters , MA
  • Identifier: vital:8484 , http://hdl.handle.net/10948/d1021038
  • Description: In the chapters that follow, the porous boundary between the public and the private in Jeremy Cronin’s poetry is investigated in his three collections, Inside (1983), Even the Dead: Poems, Parables and a Jeremiad (1996) and More Than a Casual Contact (2006). I argue two particular Marxist theorists are central to reading Cronin’s poetry: Bertolt Brecht, and his notion of the Verfremdungseffekt, and Walter Benjamin and his work on historical materialism, primarily the essay On the Concept of History / Theses on the Philosophy of History (1940). Both theorists focus on the work of art in a historically contextualized manner, which extends the challenge to the boundary between the public and the private. Their work is underpinned by the desire to draw out hidden narratives occluded under the grand narratives of history and capitalist ideas of progress. I argue that these are the major preoccupations in Cronin’s oeuvre as well. As such Cronin’s poetry may be seen to write against a perspective that proposes a linear conceptualisation of history. The poetry therefore challenges the notion that art speaks of ‘universal truths.’ Such ideas of History and Truth, if viewed uncritically, allow for a tendency to conceive of the past as unchanging, which subconsciously promotes the idea that social and political realities are merely logical evolutionary steps. I argue that Cronin’s poetry is thus purposefully interruptive in the way that it confronts the damaging consequences of the linear conceptualisation of history and the universal truth it promotes. His work attempts to find new ways of connection and expression through learning from South Africa’s violent past. The significance of understanding each other and the historical environment as opposed to imposing perspectives that underwrite the symbolic order requires the transformation rather than the simple transferral of power, and is a central focus throughout Cronin’s oeuvre. This position suggests that while the struggle for political freedom may be over, the necessity to rethink how South Africans relate to each other is only beginning. Chapter One will focus on positioning Cronin, the poet and public figure, in South African literature and literary criticism. In this regard, two general trends have operated as critical paradigms in the study of South African poetry, namely Formalism (or ‘prac crit’) and a Marxist inflected materialism, which have in many ways perpetuated the division between the private and the public. This has resulted in poetry being read with an exclusive focus on either one of these two aspects, overlooking the possibilities of dialogue that may take place between them. Cronin’s perspective on these polarised responses will be discussed, which will illustrate the similarity of his position to Ndebele’s notion of the ‘ordinary’ which suggests a way beyond these binaries. This will lead to a discussion of how South African poets responded to the transition phase, suggesting that the elements of the polarisation still remained. Considering the major influences and paradigms when reading Cronin’s oeuvre provides a foundation for the following three chapters. These include Cronin’s use of Romanticism, Bertolt Brecht and the V-Effekt and Walter Benjamin’s perspectives on historical materialism. In addition to these three theoretical paradigms, the relevance of Pablo Neruda’s poetry to Cronin’s work is also foregrounded. In Chapter Two, the focus will be on Cronin’s first collection of poetry, Inside, concentrating on Cronin’s use of language as a way of constructing poetry in the sparseness of the prison experience. This will show an abiding preoccupation of learning to speak in a language that considers the material context out of which it emerges. In this regard, the poems “Poem-Shrike” “Prologue” and “Cave-site” are analysed. In addition, one of the central poems in Cronin’s oeuvre, “To learn how to speak […],” will be examined in order to illustrate how the poet extends this project on a meta-poetic level, asking for South African poets to ‘learn how to speak’ in the voices of South African experience and histories. I will show how this is linked to Cronin’s “Walking on Air” which illustrates how the V-Effeckt recovers the small private histories through re-telling the life story of James Matthews, a fellow prisoner incarcerated for his anti-apartheid activism, revealing how this story is intimately connected to the public sphere. In Chapter Three, Cronin’s second collection: Even the Dead: Poems, Parables and a Jeremiad will be examined. In the poem “Three Reasons for a Mixed, Umrabulo, Round-the-Corner Poetry” Cronin resists inherited Western poetic conventions by incorporating and subverting versions of the Romantic aesthetic, arguing for poetry to be immersed in South African multi-lingual and multi-cultural experiences. “Even the Dead” reveals how Cronin uses Walter Benjamin’s perspectives on historical materialism to confront amnesia. In terms of the themes established in “To learn how to speak […]”, the poem “Moorage” demonstrates how the public and private can never be separated in Cronin’s work. The final section of this chapter will examine how Cronin responds to Pablo Neruda’s poems “I am explaining a few things” and “The Education of a Chieftain,” and how these poems challenge narratives that privilege the ‘great leader’ instead of the so-called smaller individuals’ stories. Chapter Four examines selections from Cronin’s third collection, focusing on Cronin’s use of the automobile, charting an ambiguous trajectory through the ‘new’ South Africa. The examination of the poems “Where to begin?”, “Switchback” and “End of the century - which is why wipers,” all attempt to include individuals left on the margins of the narrative of global freeways and neo-liberal capitalist progress. The poems present an interrogation of how ‘vision’ is constructed. This will show that the poetry responds to the experiences of the marginalised under these grand narratives in a primarily fragmentary and interruptive manner. This in effect constitutes the culmination of Cronin’s poetic journey and the search for new ways of envisaging South Africa’s future and finding a new language with which to speak it.
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  • Date Issued: 2014

Crumpled hearts

  • Authors: Crain, Patricia Ann
  • Date: 2014
  • Subjects: South African fiction (English) -- 21st century , Creative writing (Higher education) , English language -- Writing , South African fiction (English) -- Study and teaching (Higher)
  • Language: English
  • Type: Thesis , Masters , MA
  • Identifier: vital:5984 , http://hdl.handle.net/10962/d1015676
  • Description: A middle-aged woman, living in Johannesburg, has an experience which catapults her into changing her life. In the process of confronting her alcoholism, she realizes how patterns of addiction extend to other areas of her life and tries to make sense of the tragic events that have occurred. Her world becomes a different place as she questions everything that she has been taught about relationships, religion, race and her place in society. In the search for answers she uncovers stories about the lives of her parents, grandparents, relatives, friends and acquaintances. Embarking on a journey of discovery and rediscovery through her experiences and those of others, she explores the ways in which the things that she thought she knew defined her behaviour and expression of herself.
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  • Date Issued: 2014

Exploring the impact of agricultural project and income generation in Uitenhage

  • Authors: Kwesaba, Xolelwa
  • Date: 2014
  • Subjects: Agricultural development projects -- South Africa -- Uitenhage , Community development -- South Africa -- Uitenhage , Economic assistance, Domestic -- South Africa -- Uitenhage
  • Language: English
  • Type: Thesis , Masters , MA
  • Identifier: vital:9234 , http://hdl.handle.net/10948/d1021076
  • Description: An income generating project signifies an unemployment strategy and an attempt at upliftment of living standards This study tries to explore the impact of agricultural and income generating projects on poverty alleviation in the Uitenhage area .The research topic tries to establish whether these poverty alleviation projectshave impacted sufficiently and contributed to socio-economic development and eradication of poverty . A sample of 15 respondents involved in an income generating project funded by the Department of Social Development was drawn from 1 Tinarha Agricultural Tourism Initiative. A purposive samplingmethod was used for thisstudy. Data was gathered through semi-structured questionnaires. Related literature that focuses on income generating projects for poverty alleviation has been reviewed. Various recommendations based on the findings of the study have also been made. The findings of the study indicate that the income generation project, has not impacted and contributed significantly to the process of eradicating poverty for the project members. It also indicates that project members were not empowered sufficiently with financial management skills needed to equip them to make substantial profits from their small business enterprises. It also emerged that the members were not able to secure markets for their products. This remains a key challenge for attainingsustainability. The research findings indicated clearly that the strategies that were used by the Departments were not effective enough to achieve the desired goals of poverty alleviation.
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  • Date Issued: 2014

Exploring the challenges of income generating projects funded by the Department of Social Development at Kwa-Nobuhle, Uitenhage, in the Eastern Cape

  • Authors: Filita, Unathi Samora
  • Date: 2013
  • Subjects: Public investments -- South Africa -- Port Elizabeth , Economic development projects -- South Africa -- Port Elizabeth , Poverty -- South Africa -- Port Elizabeth -- Economic conditions
  • Language: English
  • Type: Thesis , Masters , MA
  • Identifier: vital:9191 , http://hdl.handle.net/10948/d1020323
  • Description: Income generating projects represent one strategy adopted by the Department of Social Development to alleviate poverty. The purpose of this study is to explore challenges of an income generating project funded by the Department of Social Development in Kwa-Nobuhle, Uitenhage of the Eastern Cape Province of South Africa., A sample of thirty respondents was drawn from ten income generating projects funded by the Department of Social Development. The sampling method of this study was purposive sampling. Data was gathered through semi-structured questionnaires. Related literature has been reviewed that focuses on income generating projects for poverty alleviation. Various recommendations have been made on the findings of the study. The findings of the study indicated the need for regular visits from the officials of the Department, to conduct monitoring and evaluation. Project members also need training on financial management so they cannot mismanage their finances. The research findings indicated clearly that strategies that were used by the Department were not effective enough to achieve desired goals of poverty alleviation.
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  • Date Issued: 2013

Role of public participation in the intergrated development plan in local government : the case of Nelson Mandela Bay Municipality

  • Authors: Mahlinza, Nozipho Clarice
  • Date: 2013
  • Subjects: Metropolitan government -- South Africa -- Port Elizabeth , Local government -- South Africa -- Port Elizabeth , Public administration -- South Africa -- Port Elizabeth , Economic development -- South Africa -- Port Elizabeth
  • Language: English
  • Type: Thesis , Masters , MPA
  • Identifier: vital:8316 , http://hdl.handle.net/10948/d1020109
  • Description: Local Government has a critical role to play in rebuilding local communities and environments as the basis for a democratic, with specific reference to the Nelson Mandela Bay Municipality. The main aim was to evaluate the public participation in the Integrated Development Plan in the NMBM and whether public participation is seen as vehicle to enhance policy formulation and implementation of the Integrated Development Plan. To provide strategies to remedy the situation. The sample comprised of senior officials, Mayoral Committee, Ward councillors, and officials who deals with IDP. Questionnaires with open and closed questions were employed to councillors and officials, interviews were conducted with senior officials, ward councillors and members in the ward. Findings suggest that the current public participation strategies are inadequate, because Budget and IDP processes are not properly integrated; the plans approved by council are not adhered to resulting to fighting fires. Based on the research findings, councillors and political leaders should consider structuring and moderating the public debate in the IDP process to ensure IDP is real and above all implemental. Finally internal communication between the administrative and political arms of the NMBM needs to be managed in a way that builds trust and understanding of the key process such as the IDP cycle, mid‐term and annual budget cycle.
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  • Date Issued: 2013

The role of financial regulators in the Kenyan economy

  • Authors: Khakali, Linda Anyoso
  • Date: 2013
  • Subjects: Financial services industry -- Law and legislation Financial institutions -- Law and legislation Financial services industry -- Law and legislation -- Kenya
  • Language: English
  • Type: Thesis , Masters , MA
  • Identifier: http://hdl.handle.net/10948/11114 , vital:26886
  • Description: Financial regulation is a subject that is more often than not regarded as distant and yet another level of bureaucracy that has to be endured by both the public and private sectors. The significance of creating and maintaining an efficient and effective system to regulate financial markets, financial institutions and financial service providers is a salient feature in the development of a country’s economic health. The recent global economic crises of 2007/2008 and the economic hurdles accompanying those events are perhaps the most dramatic instances of how necessary the implementation of efficient and effective financial regulation is. The international financial system has experienced a retinue of changes in the last two decades. One of the main challenges of financial regulators has been to keep abreast of as well as adapt to these changes, which are of an international nature. In a majority of countries, the financial sector is one of the most intensely regulated and supervised industries. Over a period of time, it has become evident that regulatory arrangements have a formidable impact on: i. The size, structure and efficiency of a financial system; ii. The business operations of financial institutions and markets; iii. Competitive conditions both overall and between sub-sectors of the system. The impact of regulation can either be stagnant or progressive; this depends on how the objectives of regulation are defined and how efficiently regulatory arrangements are related to their objectives. The issue at hand is to engage regulatory institutions, structures and mechanisms for supervision and enforcement need to be implemented because they are pertinent to the formal regulatory requirements in the overall regulatory regime. Effective financial regulation would be unable to exert its objectives in the absence of efficient supervision and enforcement. In numerous countries the institutional structure of regulation has experienced change or is in the process of change. Different models of institutional structure are availed such as the single/consolidated model, the twin-peak model and the multiple regulator model. For example, the United Kingdom has embraced the single/consolidated regulator model while Australia has employed the twin-peak regulator model. Kenya operates on the multiple regulator model. This report addresses the role of financial regulators in the Kenyan economy. The objectives of the research are to: Provide comprehensive information about the theory and practice of financial regulation; Identify the financial regulators in Kenya and define their roles; Address the issue of multiple regulators and the duplicity of roles; Discuss international trends in regulation and examine different regulatory regimes; Consider the viability of a single/consolidated regulatory regime in Kenya; Suggest a possible future regulatory regime for Kenya and identify the key issues associated with such a regime; Suggest areas for further investigation and research.The approach of this report will constitute the following: Chapter 1 discusses the rationale for the research, objectives, scope and scale of the research, preliminary literature review and the research methods to be employed. Chapter 2 focuses on financial regulatory systems in general as well as an extensive analysis of financial regulators in Kenya. Chapter 3 combines the research methods employed and also contains a comparative analysis of the regulatory regime. Chapter 4 examines the findings of the research, the lessons learnt and the regulatory responses. Chapter 5 includes recommendations towards improvement of regulatory systems and an executive summary of outstanding policy issues and priorities in Kenyan financial regulation.
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  • Date Issued: 2013

Understanding Generation Y : an investigation of how Hilton College can use technology for communication

  • Authors: Wyngaard, Richard Clive
  • Date: 2013
  • Subjects: Generation Y -- South Africa -- KwaZulu-Natal Cell phones -- Social aspects Mobile communication systems -- Social aspects Information technology -- South Africa -- KwaZulu-Natal
  • Language: English
  • Type: Thesis , Masters , MBA
  • Identifier: vital:796 , http://hdl.handle.net/10962/d1004332
  • Description: Background to the academic research. Hilton College is an all boys private school in the Kwa-Zulu Natal Midlands. Since the beginning of 2012 boys have been permitted to carry their phones during the school day, (Thomson, 2012). Wireless internet access was also extended across the campus to allow boys and staff to bring their own devices to class and have access to the internet (Machlachlan, 2012). In the past, communication between staff and boys was either verbal or through the use of a daily notice, called the Daily Routine Order (DRO). The DRO is placed at the dining room tables each day and boys are expected to check for any notices that may pertain to them. These notices are emailed to a secretary who prints them out and places them in the dining room each day at lunch. 2. Objectives to the research. This research sought to establish whether current communication methods were effective or whether the prevalence of mobile phones and wireless devices had presented the school with a more effective method. It was important therefore, to investigate what devices were on campus, how those devices were used and when they were used. In addition to this, the research sought to establish communication preference among staff and boys. 3. Research Methodology. The research was conducted through the use of a qualitative survey that was conducted among both staff and boys. Staff and boys were surveyed to establish generation, device prevalence, device usage and device preference. This data was collected using an anonymous qualitative survey that was administered through the use of Google docs in the school labs. 187 Boys in 3 grades 8 and 9 participated in the survey along with 18 staff members. The data was then analysed and compared to data from similar research that had been conducted elsewhere in the world. Because of significant age differences between boys and staff it was critical to establish whether device usage among staff and boys was tied to their generation. Of particular interest were the device behaviours associated with the Millennial generation, who were born between 1982-2002 (Strauss 2005:10). This generational ‘benchmark’ allowed the researcher to compare the Hilton College cohort to those whose device behaviours and usage had occurred and been discussed in generational research elsewhere. It also allowed for certain assumptions to be made about staff and boys based on their generational profile. 4. Findings It was found that staff and boys exhibited communication traits that were consistent with their generation and consistent with other research in this field. It was also found that staff and boys preferred mobile and digital forms of communication over verbal announcements and the DRO system. For this reason, it was recommended that the Hilton College establish a system that took advantage of technology to improve on communication. Various recommendations were given by the researcher that took advantage of current technologies. These included the use of SMS, Instant Messaging, Social Networks and email. It was also recommended that the school consider prescribing devices that allow for communication between staff and boys.
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  • Date Issued: 2013

Synthesis of bromochloromethane using phase transfer catalysis

  • Authors: Brooks, Lancelot L
  • Date: 2011
  • Subjects: Chemistry, Analytic , Fire extinguishing agents , Chemical systems , Physical science
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:10382 , http://hdl.handle.net/10948/d1008162 , Chemistry, Analytic , Fire extinguishing agents , Chemical systems , Physical science
  • Description: The synthesis of bromochloromethane (BCM) in a batch reactor, using phase transfer catalysis, was investigated. During the synthetic procedure, sodium bromide (100.0g, 0.97mol) along with an excess amount of dichloromethane (265.0g, 3.12 mol) was charged to a reactor containing benzyl triethylammonium chloride (13 mmol), dissolved in 50 ml of water. The bench scale reactions were all carried out in a Parr 4520 bench top pressure reactor coupled to a Parr 4841 temperature controller. The method produced a 50.0 percent yield of the product BCM after a reaction time of 12 to 13 hours. The main objective for this investigation was to optimize the abovementioned reaction with respect to yield and reactor throughput. Quantitative analysis of BCM was performed on a Focus Gas Chromatograph, fitted with a flame ionization detector, and a BP20 column (30m × 0,32mm ID × 0,25 mm). Delta software, version 5.0, was applied for data collection and processing. The injector and detector port were set at 250°C and 280°C, respectively. The oven temperature was set and held at 40°C for a period of 2 minutes, then gradually increased at a rate of 10°C/min to 130°C, with the final hold time set for 1 minute. An analytical method for the quantitative analysis of BCM was developed, optimized and validated. Validation of the analytical method commenced over a period of three days, and focussed the following validation parameters: Accuracy, precision, and ruggedness. Statistical evaluation of the results obtained for precision showed that the error between individual injections is less than 2 percent for each component. However, ANOVA analysis showed a significant difference between the mean response factors obtained in the three day period (p-value < 0.05). Thus we could conclude that the response factors had to be determined on each day before quantitatively analyzing samples. The accuracy of the analytical method was assessed by using the percent recovery method. Results obtained showed that a mean percent recovery of 100.18 percent was obtained for BCM, with the absolute bias = 0.0004, and the percent bias = 0.18 percent. Hence the 95 confidence intervals for the percent recovery and percent bias are given by: (Lz, Uz) = (100.56 percent percent 102.15 percent), 13 (LPB, UPB) = (0.56 percent, 2.15 percent), respectively. Since the 95 percent confidence interval for the percent recovery contains 100, or equivalently, the 95 percent confidence interval for percent bias contains 0, the assay method is considered accurate and validated for BCM. In the same manner the accuracy and percent recovery for DCM and DBM was evaluated. The method was found to be accurate and validated for DBM, however, slightly biased in determining the recovered amount of DCM. With the analytical method validated, the batch production process could be evaluated. A total of six process variables, namely reaction time, water amount, temperature, volume of the two phases, stirring rate, and catalyst concentration, were selected for the study. The effects of the individual variables were determined in the classical manner, by varying only the one of interest while keeping all others constant. The experimental data generated was fit to a quadratic response surface model. The profile plots that were obtained from this model allowed a visual representation of the effect of the six variables. The experimental results obtained showed that the reaction follows pseudo zero-order kinetics and that the rate of the reaction is directly proportional to the concentration of the catalyst. The reaction obeys the Arrhenius equation, and the relatively high activation energy of 87kJ.mol -1 signifies that the rate constant is strongly dependent on the temperature of the reaction. The results also showed that the formation of BCM is favoured by an increase in the reaction temperature, catalyst concentration, and a high organic: aqueous phase ratio. Thus the synthesis of BCM using phase transfer catalyst could be optimised, to obtain a 100 percent yield BCM, by increasing both the reaction temperature to 105°C, and the concentration of the phase transfer catalyst -benzyl triethylammonium chloride - to 5.36 mol percent. The reaction time was also reduced to 6 hours.
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  • Date Issued: 2011
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