Trypanosoma brucei J protein 2 is a stress inducible and essential Hsp40
- Ludewig, Michael H, Boshoff, Aileen, Horn, David, Blatch, Gregory L
- Authors: Ludewig, Michael H , Boshoff, Aileen , Horn, David , Blatch, Gregory L
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/431798 , vital:72804 , xlink:href="https://doi.org/10.1016/j.biocel.2014.12.016"
- Description: Hsp40 proteins (also known as DnaJ or J proteins) serve as co-chaperones for Hsp70, but also display evidence of independent chaperone function. Furthermore, certain Hsp40s have been shown to be stress-inducible and essential. Trypanosomatids display a remarkable diversification of Hsp40 proteins, with numerous distinct Hsp40-like proteins encoded in the Trypanosoma brucei genome. This study investigated the role of one of the six T. brucei Type I Hsp40s, T. brucei J protein 2 (Tbj2). We found that Tbj2 was heat stress-inducible, and that knockdown using RNA interference resulted in a severe growth defect under normal growth temperatures. Furthermore, a green fluorescent protein (GFP)-Tbj2 fusion protein was found to be localized to the cytosol of T. brucei. Taken together, these data suggest that Tbj2 is not functionally equivalent to the other five Type I Hsp40s, and that it is an essential, cytosolic, and stress-inducible chaperone, potentially playing an important role in protein biogenesis in T. brucei.
- Full Text:
- Date Issued: 2015
- Authors: Ludewig, Michael H , Boshoff, Aileen , Horn, David , Blatch, Gregory L
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/431798 , vital:72804 , xlink:href="https://doi.org/10.1016/j.biocel.2014.12.016"
- Description: Hsp40 proteins (also known as DnaJ or J proteins) serve as co-chaperones for Hsp70, but also display evidence of independent chaperone function. Furthermore, certain Hsp40s have been shown to be stress-inducible and essential. Trypanosomatids display a remarkable diversification of Hsp40 proteins, with numerous distinct Hsp40-like proteins encoded in the Trypanosoma brucei genome. This study investigated the role of one of the six T. brucei Type I Hsp40s, T. brucei J protein 2 (Tbj2). We found that Tbj2 was heat stress-inducible, and that knockdown using RNA interference resulted in a severe growth defect under normal growth temperatures. Furthermore, a green fluorescent protein (GFP)-Tbj2 fusion protein was found to be localized to the cytosol of T. brucei. Taken together, these data suggest that Tbj2 is not functionally equivalent to the other five Type I Hsp40s, and that it is an essential, cytosolic, and stress-inducible chaperone, potentially playing an important role in protein biogenesis in T. brucei.
- Full Text:
- Date Issued: 2015
Uncle Noodle
- Authors: Krueger, Anton
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/229676 , vital:49699 , xlink:href="https://hdl.handle.net/10520/EJC176906"
- Description: “Ah,” he seemed surprised to see me, “Now you find out who really cares...” His kidneys hadn‟t been on the job in months, and with the machines failing, his hands were fattening into stubby yellow fingers, as the waste shored up inside of him. I touched his arm, and we joked about the broad blunt strokes of the casiotone the old dame played for the lunch-eaters next door. Uncle Noodle had always been my favourite. In a world where adults were always certain, ready to disdain and judge and pass verdict – I loved it that he was indecisive, insecure, unsure. Often wretched, often defeated. His heart had chasmed in the wake of his wife‟s leaving, finally dividing him from all his hopes, collapsing his dignity destroying his happiness machinery.
- Full Text:
- Date Issued: 2015
- Authors: Krueger, Anton
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/229676 , vital:49699 , xlink:href="https://hdl.handle.net/10520/EJC176906"
- Description: “Ah,” he seemed surprised to see me, “Now you find out who really cares...” His kidneys hadn‟t been on the job in months, and with the machines failing, his hands were fattening into stubby yellow fingers, as the waste shored up inside of him. I touched his arm, and we joked about the broad blunt strokes of the casiotone the old dame played for the lunch-eaters next door. Uncle Noodle had always been my favourite. In a world where adults were always certain, ready to disdain and judge and pass verdict – I loved it that he was indecisive, insecure, unsure. Often wretched, often defeated. His heart had chasmed in the wake of his wife‟s leaving, finally dividing him from all his hopes, collapsing his dignity destroying his happiness machinery.
- Full Text:
- Date Issued: 2015
Unravelling the myths about unplanned pregnancy among female students at University of Fort Hare East London Campus, South Africa
- Authors: Chukwunyere , Amadi P
- Date: 2015
- Subjects: Unwanted pregnancy , Women college students , College students
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10353/25415 , vital:64237
- Description: This research study examined: 1. the attitudes and perceptions of female university students towards unplanned pregnancies, their (pregnancies’) associated influences and their social economic consequences on the participants and their family. 2. The level of knowledge, the sources thereof, and the girls’ attitudes and perceptions toward contraception, abortion and their actual use as possible barriers in the prevention of unplanned pregnancies. 3. The possible interventions in curbing the phenomenon of unplanned pregnancies. Data for the study was gathered through in-depth-interviews with ten female undergraduate students at the University of Fort Hare in East London campus, who had the experience of unplanned pregnancy while studying. Selection of participants was done through snowball sampling. A number of factors responsible for the unplanned pregnancy are revealed, including: Inadequate and inconsistent use of contraceptives, ignorance about contraception or rejection of contraceptive use due to religious beliefs. Influential factors regarding unplanned child bearing include: The role of the mother and the boyfriend, traditional and religious beliefs, and the desire for child bearing and motherhood. Although the majority of the girls are knowledgeable about contraception, and their right to abortion, a number of barriers hinder them from putting this knowledge into practical use. Some of the most highlighted consequences of unplanned/unwanted pregnancy are the financial burden on the family and loss of study time. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2015
- Full Text:
- Date Issued: 2015
- Authors: Chukwunyere , Amadi P
- Date: 2015
- Subjects: Unwanted pregnancy , Women college students , College students
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10353/25415 , vital:64237
- Description: This research study examined: 1. the attitudes and perceptions of female university students towards unplanned pregnancies, their (pregnancies’) associated influences and their social economic consequences on the participants and their family. 2. The level of knowledge, the sources thereof, and the girls’ attitudes and perceptions toward contraception, abortion and their actual use as possible barriers in the prevention of unplanned pregnancies. 3. The possible interventions in curbing the phenomenon of unplanned pregnancies. Data for the study was gathered through in-depth-interviews with ten female undergraduate students at the University of Fort Hare in East London campus, who had the experience of unplanned pregnancy while studying. Selection of participants was done through snowball sampling. A number of factors responsible for the unplanned pregnancy are revealed, including: Inadequate and inconsistent use of contraceptives, ignorance about contraception or rejection of contraceptive use due to religious beliefs. Influential factors regarding unplanned child bearing include: The role of the mother and the boyfriend, traditional and religious beliefs, and the desire for child bearing and motherhood. Although the majority of the girls are knowledgeable about contraception, and their right to abortion, a number of barriers hinder them from putting this knowledge into practical use. Some of the most highlighted consequences of unplanned/unwanted pregnancy are the financial burden on the family and loss of study time. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2015
- Full Text:
- Date Issued: 2015
Using GIS and remote sensing to map suitable sites for wind farms: a case study of Nkonkobe Municipality, Eastern Cape.
- Authors: Gwena, Jay
- Date: 2015
- Subjects: Geographic information systems http://id.loc.gov/authorities/subjects/sh90001880 , Remote sensing http://id.loc.gov/authorities/subjects/sh85112798 , Renewable energy sources http://id.loc.gov/authorities/subjects/sh85112837
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/19420 , vital:43082
- Description: Issues relating to energy use, renewable energy introduction and climate change have received a lot of attention from governments throughout the world in the past two decades. Many developing nations like South Africa heavily rely on coal and other non-renewable resources for their production of energy. The processing of the non-renewable resources into secondary energy forms is according to many energy experts a chief contributor to climate change. Climate change is affecting agricultural production in many developing countries that entirely rely on rainfall for their production. The loss in production in South Africa is increasing the level of poverty in low-income earners and unemployed people. Also a lot of households found in rural areas of South Africa have no access to electricity. Electricity in South Africa is generated and distributed by Eskom a state owned company. In April 2008 Eskom failed to meet power demand and started introducing load shedding to maintain and upgrade their power plants. The load shedding stopped for while and was introduced again in 2014 when the power utility faced the challenge of failing to produce power to meet demand again due to problems with their power stations. The ongoing energy provision problems in South Africa can be alleviated by introducing alternative sources like renewable energy. Introduction of renewable energy in poorly developed areas like Nkonkobe Municipality can help households‘ access electricity and reduce load on the national grid. The aim of this study was to locate suitable sites for setting up wind farms in Nkonkobe municipality in South Africa. Wind energy as a resource is abundant in many areas of South Africa and areas to set wind farms for optimum harness of wind power were identified using GIS, remote sensing and multi-criteria decision making techniques. Wind speed, distance from settlements, distance from main roads, distance from national, slope and land use/land cover were chosen as the factors to consider in selecting a suitable site. Data was collected from South Africa Weather Services, University of Fort Hare and online free data sources. Thematic maps for all the factors were developed in ArcGIS and fed into Nkonkobe Municipality database developed for the study. Thematic maps were assigned weights before being overlaid using weighted overlay tool. Weights for the factors were determined using Analytical hierarchy Process‘ pairwise comparison approach. Weighted overlay of the thematic maps produced a map showing suitable areas based on the weight influence of each factor. The output map was compared to results obtained from using Boolean approach and weighted sum method as a way of checking the accuracy of obtained suitable sites. Results showed there are three sites that can be used for setting up wind farms in the study area. , Thesis (MSc) (Applied Remote Sensing and GIS) -- University of Fort Hare, 2015
- Full Text:
- Date Issued: 2015
- Authors: Gwena, Jay
- Date: 2015
- Subjects: Geographic information systems http://id.loc.gov/authorities/subjects/sh90001880 , Remote sensing http://id.loc.gov/authorities/subjects/sh85112798 , Renewable energy sources http://id.loc.gov/authorities/subjects/sh85112837
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/19420 , vital:43082
- Description: Issues relating to energy use, renewable energy introduction and climate change have received a lot of attention from governments throughout the world in the past two decades. Many developing nations like South Africa heavily rely on coal and other non-renewable resources for their production of energy. The processing of the non-renewable resources into secondary energy forms is according to many energy experts a chief contributor to climate change. Climate change is affecting agricultural production in many developing countries that entirely rely on rainfall for their production. The loss in production in South Africa is increasing the level of poverty in low-income earners and unemployed people. Also a lot of households found in rural areas of South Africa have no access to electricity. Electricity in South Africa is generated and distributed by Eskom a state owned company. In April 2008 Eskom failed to meet power demand and started introducing load shedding to maintain and upgrade their power plants. The load shedding stopped for while and was introduced again in 2014 when the power utility faced the challenge of failing to produce power to meet demand again due to problems with their power stations. The ongoing energy provision problems in South Africa can be alleviated by introducing alternative sources like renewable energy. Introduction of renewable energy in poorly developed areas like Nkonkobe Municipality can help households‘ access electricity and reduce load on the national grid. The aim of this study was to locate suitable sites for setting up wind farms in Nkonkobe municipality in South Africa. Wind energy as a resource is abundant in many areas of South Africa and areas to set wind farms for optimum harness of wind power were identified using GIS, remote sensing and multi-criteria decision making techniques. Wind speed, distance from settlements, distance from main roads, distance from national, slope and land use/land cover were chosen as the factors to consider in selecting a suitable site. Data was collected from South Africa Weather Services, University of Fort Hare and online free data sources. Thematic maps for all the factors were developed in ArcGIS and fed into Nkonkobe Municipality database developed for the study. Thematic maps were assigned weights before being overlaid using weighted overlay tool. Weights for the factors were determined using Analytical hierarchy Process‘ pairwise comparison approach. Weighted overlay of the thematic maps produced a map showing suitable areas based on the weight influence of each factor. The output map was compared to results obtained from using Boolean approach and weighted sum method as a way of checking the accuracy of obtained suitable sites. Results showed there are three sites that can be used for setting up wind farms in the study area. , Thesis (MSc) (Applied Remote Sensing and GIS) -- University of Fort Hare, 2015
- Full Text:
- Date Issued: 2015
Venerating death
- Authors: Jones, Ward E
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/275749 , vital:55076 , xlink:href="https://doi.org/10.1080/05568641.2015.1014540"
- Description: In this paper, I am concerned with elucidating and expanding our attitudes toward our own death. As it is, our common attitudes toward our death are the following: we fear our premature death, and we dread our inevitable death. These attitudes are rational, but I want to argue that our attitudes toward death should be more complicated than this. A condition upon our value, our preciousness, as creatures is that we are vulnerable, and our vulnerability is, at bottom, a vulnerability to death. A corollary of this is that we could not be loved, either by ourselves or by others, for one cannot love—be concerned for—a being invulnerable to death. As a consequence, death plays a deep and abiding role in our value systems. Our susceptibility to premature and inevitable death is a condition upon our being valuable creatures and, in turn, it is a condition upon our being loved. Given the high value that we place on being valuable creatures who deserve love, we should equally place a high value on the constitutive conditions for being precious and loved. If, as I suggest, one of these conditions is that we will die, we should see our deaths not simply as something to fear or dread, but as something of great importance in our lives. Our deaths should be treated with awe, respect, and even praise.
- Full Text:
- Date Issued: 2015
- Authors: Jones, Ward E
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/275749 , vital:55076 , xlink:href="https://doi.org/10.1080/05568641.2015.1014540"
- Description: In this paper, I am concerned with elucidating and expanding our attitudes toward our own death. As it is, our common attitudes toward our death are the following: we fear our premature death, and we dread our inevitable death. These attitudes are rational, but I want to argue that our attitudes toward death should be more complicated than this. A condition upon our value, our preciousness, as creatures is that we are vulnerable, and our vulnerability is, at bottom, a vulnerability to death. A corollary of this is that we could not be loved, either by ourselves or by others, for one cannot love—be concerned for—a being invulnerable to death. As a consequence, death plays a deep and abiding role in our value systems. Our susceptibility to premature and inevitable death is a condition upon our being valuable creatures and, in turn, it is a condition upon our being loved. Given the high value that we place on being valuable creatures who deserve love, we should equally place a high value on the constitutive conditions for being precious and loved. If, as I suggest, one of these conditions is that we will die, we should see our deaths not simply as something to fear or dread, but as something of great importance in our lives. Our deaths should be treated with awe, respect, and even praise.
- Full Text:
- Date Issued: 2015
Visible light transformation of Rhodamine 6G using tetracarbazole zinc phthalocyanine when embedded in electrospun fibers and in the presence of ZnO and Ag particles
- Khoza, Phindile, Nyokong, Tebello
- Authors: Khoza, Phindile , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189456 , vital:44848 , xlink:href="https://doi.org/10.1080/00958972.2015.1013944"
- Description: Herein, we report the photocatalytic transformation of Rhodamine 6G (Rh 6G) using tetracarbazole zinc phthalocyanine (TCbZnPc) when alone or when conjugated with ZnO macroparticles (ZnOMPs) and silver nanoparticles (AgNPs), represented as TCbZnPc–ZnOMPs and TCbZnPc–AgNPs, respectively. The photocatalysts were supported onto electrospun polystyrene fibers. The efficiency of TCbZnPc was improved by the presence of both ZnOMPs and AgNPs. HPLC equipped with UV–vis was used to study phototransformation products. The mechanism of transformation was via the N-de-ethylation of Rh 6G.
- Full Text:
- Date Issued: 2015
- Authors: Khoza, Phindile , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189456 , vital:44848 , xlink:href="https://doi.org/10.1080/00958972.2015.1013944"
- Description: Herein, we report the photocatalytic transformation of Rhodamine 6G (Rh 6G) using tetracarbazole zinc phthalocyanine (TCbZnPc) when alone or when conjugated with ZnO macroparticles (ZnOMPs) and silver nanoparticles (AgNPs), represented as TCbZnPc–ZnOMPs and TCbZnPc–AgNPs, respectively. The photocatalysts were supported onto electrospun polystyrene fibers. The efficiency of TCbZnPc was improved by the presence of both ZnOMPs and AgNPs. HPLC equipped with UV–vis was used to study phototransformation products. The mechanism of transformation was via the N-de-ethylation of Rh 6G.
- Full Text:
- Date Issued: 2015
Whose voice is it anyway?
- Authors: Krueger, Anton
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/225695 , vital:49249 , xlink:href="https://doi.org/10.1080/1013929X.2015.1086195"
- Description: This essay surveys a number of different interpretations of the metaphor of “voice”. It begins by exploring the use of free writing exercises as a means of nurturing the emergence of physical (audible) voice in creative writing classes before assessing some of the ramifications and implications of the trope, both diachronically and synchronically. A key issue of this discussion is whether voice is regarded as individual or social.
- Full Text:
- Date Issued: 2015
- Authors: Krueger, Anton
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/225695 , vital:49249 , xlink:href="https://doi.org/10.1080/1013929X.2015.1086195"
- Description: This essay surveys a number of different interpretations of the metaphor of “voice”. It begins by exploring the use of free writing exercises as a means of nurturing the emergence of physical (audible) voice in creative writing classes before assessing some of the ramifications and implications of the trope, both diachronically and synchronically. A key issue of this discussion is whether voice is regarded as individual or social.
- Full Text:
- Date Issued: 2015
Wisdom as an aim of higher education
- Authors: Jones, Ward E
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/275765 , vital:55077 , xlink:href="https://doi.org/10.1007/s10790-014-9443-z"
- Description: A central concern of theoretical speculation about education is the kind of epistemic states that education can and should aim to achieve. One such epistemic state, long neglected in both education theory and philosophy, is wisdom. Might wisdom be something that educators should aim for? And might it be something that their students can achieve? My answer will be a qualified yes.
- Full Text:
- Date Issued: 2015
- Authors: Jones, Ward E
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/275765 , vital:55077 , xlink:href="https://doi.org/10.1007/s10790-014-9443-z"
- Description: A central concern of theoretical speculation about education is the kind of epistemic states that education can and should aim to achieve. One such epistemic state, long neglected in both education theory and philosophy, is wisdom. Might wisdom be something that educators should aim for? And might it be something that their students can achieve? My answer will be a qualified yes.
- Full Text:
- Date Issued: 2015
Women combatants and the liberation movements in South Africa
- Authors: Magadla, Siphokazi
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/298584 , vital:57718 , xlink:href="https://doi.org/10.1080/10246029.2015.1088645"
- Description: This article examines women's role as combatants in national liberation forces in South Africa. Three categories – guerrilla girls, combative mothers and the in-betweeners – are introduced to underscore the varied ways in which women have participated in combat within the national liberation movements. Factors such as age and one's ability to leave the country affected whether women could participate in combat as ‘guerrilla girls’ or if it limited them to fighting apartheid violence from home, or if there were women who can be defined as having fallen somewhere in between these categories. These categories are used to theorise women's combat roles in the anti-apartheid struggle, thus broadening and challenging the dominant notions of combat that often hide women's contributions in war. In this regard, different periods of struggle, physical location, as well as age, determined the methods of activism available to men and women.
- Full Text:
- Date Issued: 2015
- Authors: Magadla, Siphokazi
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/298584 , vital:57718 , xlink:href="https://doi.org/10.1080/10246029.2015.1088645"
- Description: This article examines women's role as combatants in national liberation forces in South Africa. Three categories – guerrilla girls, combative mothers and the in-betweeners – are introduced to underscore the varied ways in which women have participated in combat within the national liberation movements. Factors such as age and one's ability to leave the country affected whether women could participate in combat as ‘guerrilla girls’ or if it limited them to fighting apartheid violence from home, or if there were women who can be defined as having fallen somewhere in between these categories. These categories are used to theorise women's combat roles in the anti-apartheid struggle, thus broadening and challenging the dominant notions of combat that often hide women's contributions in war. In this regard, different periods of struggle, physical location, as well as age, determined the methods of activism available to men and women.
- Full Text:
- Date Issued: 2015
“Ha! Relationships? I only shout at them!”: Strategic management of discordant rapport in an African small business context
- Lauriks, Sanne, Siebörger, Ian, de Vos, Mark
- Authors: Lauriks, Sanne , Siebörger, Ian , de Vos, Mark
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/385338 , vital:68009 , xlink:href="https://doi.org/10.1515/pr-2015-0002"
- Description: This study demonstrates how and why interactants at a tyre fitment centre in Grahamstown, South Africa, manage discordant interpersonal relationships in strategic ways. Individuals in a post-apartheid small business respond to their social and economic context and exercise agency to their advantage in doing so. This study draws on linguistic ethnography (Rampton 2007) and the Rapport Management Framework (RMF, Spencer-Oatey 2000b, 2011), itself a development of politeness theory (Brown and Levinson 1987). An initial RMF analysis ran into difficulties around interactions that at first glance appeared to be oriented toward Rapport Challenge and Neglect. Upon closer examination, it appeared that discordant rapport was being actively maintained in this business. This led us to address underdeveloped areas of RMF that were not responsive enough to describe naturally occurring small business interactions, and propose an Enhanced Rapport Management Framework to overcome its inadequacies. We conclude that people may deliberately maintain discordant relationships when it is in their best interests to do so. Thus, contrary to a common-sense belief that harmonious social relations are an intrinsic good, we found that promoting discordant social relations can be understood as a rational response to individuals’ social and economic contexts, particularly in conditions such as those in many postcolonial African societies.
- Full Text:
- Date Issued: 2015
- Authors: Lauriks, Sanne , Siebörger, Ian , de Vos, Mark
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/385338 , vital:68009 , xlink:href="https://doi.org/10.1515/pr-2015-0002"
- Description: This study demonstrates how and why interactants at a tyre fitment centre in Grahamstown, South Africa, manage discordant interpersonal relationships in strategic ways. Individuals in a post-apartheid small business respond to their social and economic context and exercise agency to their advantage in doing so. This study draws on linguistic ethnography (Rampton 2007) and the Rapport Management Framework (RMF, Spencer-Oatey 2000b, 2011), itself a development of politeness theory (Brown and Levinson 1987). An initial RMF analysis ran into difficulties around interactions that at first glance appeared to be oriented toward Rapport Challenge and Neglect. Upon closer examination, it appeared that discordant rapport was being actively maintained in this business. This led us to address underdeveloped areas of RMF that were not responsive enough to describe naturally occurring small business interactions, and propose an Enhanced Rapport Management Framework to overcome its inadequacies. We conclude that people may deliberately maintain discordant relationships when it is in their best interests to do so. Thus, contrary to a common-sense belief that harmonious social relations are an intrinsic good, we found that promoting discordant social relations can be understood as a rational response to individuals’ social and economic contexts, particularly in conditions such as those in many postcolonial African societies.
- Full Text:
- Date Issued: 2015
Synthesis of phthalocyanine conjugates with gold nanoparticles and liposomes for photodynamic therapy
- Nombona, Nolwazi, Maduray, Kaminee, Antunes, Edith M, Karsten, Aletta, Nyokong, Tebello
- Authors: Nombona, Nolwazi , Maduray, Kaminee , Antunes, Edith M , Karsten, Aletta , Nyokong, Tebello
- Date: 2012
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/245798 , vital:51406 , xlink:href="https://doi.org/10.1016/j.jphotobiol.2011.11.007"
- Description: The efficiency of [2,9,17,23-tetrakis-(1,6-hexanedithiol)phthalocyaninato]zinc(II) as a photodynamic therapy (PDT) agent was investigated. This compound belongs to the second generation of photosensitizers currently tested for the cellular photo-damage of cancer cells. The production of reactive oxygen species (ROS) and phototoxicity of the photosensitizer were assessed. Healthy fibroblast cells and breast cancer (MCF-7) cells were treated with either free phthalocyanine or phthalocyanine bound to either gold nanoparticles or encapsulated in liposomes. Cell viability studies showed the optimum phototoxic effect on non-malignant cells to be 4.5 J cm−2. The PDT effect of the liposome bound phthalocyanine showed extensive damage of the breast cancer cells. Gold nanoparticles only showed a modest improvement in PDT activity.
- Full Text:
- Date Issued: 2012
- Authors: Nombona, Nolwazi , Maduray, Kaminee , Antunes, Edith M , Karsten, Aletta , Nyokong, Tebello
- Date: 2012
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/245798 , vital:51406 , xlink:href="https://doi.org/10.1016/j.jphotobiol.2011.11.007"
- Description: The efficiency of [2,9,17,23-tetrakis-(1,6-hexanedithiol)phthalocyaninato]zinc(II) as a photodynamic therapy (PDT) agent was investigated. This compound belongs to the second generation of photosensitizers currently tested for the cellular photo-damage of cancer cells. The production of reactive oxygen species (ROS) and phototoxicity of the photosensitizer were assessed. Healthy fibroblast cells and breast cancer (MCF-7) cells were treated with either free phthalocyanine or phthalocyanine bound to either gold nanoparticles or encapsulated in liposomes. Cell viability studies showed the optimum phototoxic effect on non-malignant cells to be 4.5 J cm−2. The PDT effect of the liposome bound phthalocyanine showed extensive damage of the breast cancer cells. Gold nanoparticles only showed a modest improvement in PDT activity.
- Full Text:
- Date Issued: 2012
Synthesis, photophysical and photochemical properties of octa-substituted antimony phthalocyanines
- Modibane, Desmond Kwena, Nyokong, Tebello
- Authors: Modibane, Desmond Kwena , Nyokong, Tebello
- Date: 2009
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/264226 , vital:53711 , xlink:href="https://doi.org/10.1016/j.poly.2008.11.052"
- Description: This work reports on the synthesis and photophysicochemical parameters of unsubstituted [SbIIIPc]+I3- and octa-phenoxy ([SbIIIOPPc]+I3-) and -4-t-butylphenoxy ([SbIIIOTBPPc]+I3-) substituted antimony phthalocyanines. Photophysical and photochemical properties were studied for these complexes in dimethylsulfoxide, dimethylformamide and toluene. The excitation spectra of oxidized antimony (Sb(V)Pc) derivates were similar to absorption spectra. Low fluorescence quantum yields, high triplet quantum yields and low triplet lifetimes were observed as the result of heavy atom (antimony ion).
- Full Text:
- Date Issued: 2009
- Authors: Modibane, Desmond Kwena , Nyokong, Tebello
- Date: 2009
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/264226 , vital:53711 , xlink:href="https://doi.org/10.1016/j.poly.2008.11.052"
- Description: This work reports on the synthesis and photophysicochemical parameters of unsubstituted [SbIIIPc]+I3- and octa-phenoxy ([SbIIIOPPc]+I3-) and -4-t-butylphenoxy ([SbIIIOTBPPc]+I3-) substituted antimony phthalocyanines. Photophysical and photochemical properties were studied for these complexes in dimethylsulfoxide, dimethylformamide and toluene. The excitation spectra of oxidized antimony (Sb(V)Pc) derivates were similar to absorption spectra. Low fluorescence quantum yields, high triplet quantum yields and low triplet lifetimes were observed as the result of heavy atom (antimony ion).
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- Date Issued: 2009