An investigation into development of a stable aqeous suspension of Metronidazole Benzoate for oral use
- Authors: Zietsman, Sharon Lynne
- Date: 2005
- Subjects: Metronidazole -- Testing , Excipients -- South Africa -- Testing , Rheology -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10158 , http://hdl.handle.net/10948/456 , Metronidazole -- Testing , Excipients -- South Africa -- Testing , Rheology -- South Africa
- Description: Metronidazole is a synthetic, nitroimidazole-derivative antibacterial and antiprotozoal agent (ed. McEvoy, 2001). It has been reported that crystallization occurs in aqueous suspensions of metronidazole benzoate, a bland-tasting prodrug of metronidazole, as a result of conversion from the anhydrous to the monohydrate form, thereby compromising the stability and clinical efficacy of the substance due to the particle size growth (Hoelgaard & Moller, 1983). A generic South African based pharmaceutical company commenced formulation of an aqueous metronidazole benzoate suspension and experienced problems with crystallization that occurred in products stored at 2 to 8 °C. This study aimed to continue development of the product in order to identify a formulation that prevents formation of the hydrate form of metronidazole benzoate and the accompanying crystal growth. A variety of metronidazole benzoate suspensions were manufactured on a laboratory scale using a number of natural and synthetic suspending agents, including magnesium aluminium silicate, povidone K90, xanthan gum and Avicel® RC-591 (microcrystalline cellulose and carboxymethylcellulose sodium), over a range of concentrations. Analytical quantification methods were developed and validated, and the physicochemical properties of the raw material and finished products were fully characterized. Rheological tests were performed in order to characterize the suspension flow properties. Real-time and accelerated stability studies and a temperature cycle study were conducted in accordance with the International Conference on Harmonization (ICH) guidelines. Conversion of metronidazole benzoate to the monohydrate form took place in suspensions containing xanthan gum 0.65 percent m/v under real-time and accelerated storage conditions. The suspensions containing Avicel® RC-591 were found to be physically and chemically stable after the temperature cycle and over the 12-week period whilst stored at 25 ºC / 60 percent RH and 5 ºC. The suspensions were chemically stable whilst stored at 40 ºC / 75 percent RH but showed sedimentation at this accelerated condition. The metronidazole benzoate contained in these products remained in the anhydrous state under all storage conditions and were consequently concluded to be the most stable formulations out of all the products analyzed in the current study. The suspending agent system consisting of microcrystalline cellulose and carboxymethylcellulose sodium thus shows promise in preventing the conversion of metronidazole benzoate from the anhydrate to the monohydrate form, thereby inhibited the subsequent increase in particle size due to crystal growth.
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- Date Issued: 2005
The mining and visualisation of application services data
- Authors: Knoetze, Ronald Morgan
- Date: 2005
- Subjects: Data mining -- South Africa , Computer algorithms , Network performance (Telecommunication) -- Research -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10480 , http://hdl.handle.net/10948/451 , Data mining -- South Africa , Computer algorithms , Network performance (Telecommunication) -- Research -- South Africa
- Description: Many network monitoring tools do not provide sufficiently in-depth and useful reports on network usage, particularly in the domain of application services data. The optimisation of network performance is only possible if the networks are monitored effectively. Techniques that identify patterns of network usage can assist in the successful monitoring of network performance. The main goal of this research was to propose a model to mine and visualise application services data in order to support effective network management. To demonstrate the effectiveness of the model, a prototype, called NetPatterns, was developed using data for the Integrated Tertiary Software (ITS) application service collected by a network monitoring tool on the NMMU South Campus network. Three data mining algorithms for application services data were identified for the proposed model. The data mining algorithms used are classification (decision tree), clustering (K-Means) and association (correlation). Classifying application services data serves to categorise combinations of network attributes to highlight areas of poor network performance. The clustering of network attributes serves to indicate sparse and dense regions within the application services data. Association indicates the existence of any interesting relationships between different network attributes. Three visualisation techniques were selected to visualise the results of the data mining algorithms. The visualisation techniques selected were the organisation chart, bubble chart and scatterplots. Colour and a variety of other visual cues are used to complement the selected visualisation techniques. The effectiveness and usefulness of NetPatterns was determined by means of user testing. The results of the evaluation clearly show that the participants were highly satisfied with the visualisation of network usage presented by NetPatterns. All participants successfully completed the prescribed tasks and indicated that NetPatterns is a useful tool for the analysis of network usage patterns.
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- Date Issued: 2005
Web usage mining of organisational web sites
- Authors: Oosthuizen, Craig Peter
- Date: 2005
- Subjects: Web usage mining -- South Africa , Internet users -- South Africa , Information visualization
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:9595 , http://hdl.handle.net/10948/399 , Web usage mining -- South Africa , Internet users -- South Africa , Information visualization
- Description: Web Usage Mining (WUM) can be used to determine whether the information architecture of a web site is structured correctly. Existing WUM tools however, do not indicate which web usage mining algorithms are used or provide effective graphical visualisations of the results obtained. WUM techniques can be used to determine typical navigation patterns of the users of organisational web sites. An organisational web site can be described as a site which has a high level of content. The Computer Science & Information Systems (CS&IS) web site at the Nelson Mandela Metropolitan University (NMMU) is an example of such a web site. The process of combining WUM and information visualisation techniques in order to discover useful information about web usage patterns is called visual web mining. The goal of this research is to discuss the development of a WUM model and a prototype, called WebPatterns, which allows the user to effectively visualise web usage patterns of an organisational web site. This will facilitate determining whether the information architecture of the CS&IS web site is structured correctly. The WUM algorithms used in WebPatterns are association rule mining and sequence analysis. The purpose of association rule mining is to discover relationships between different web pages within a web site. Sequence analysis is used to determine the longest time ordered paths that satisfy a user specified minimum frequency. A radial tree layout is used in WebPatterns to visualise the static structure of the organisational web site. The structure of the web site is laid out radially, with the home page in the middle and other pages positioned in circles at various levels around it. Colour and other visual cues are used to show the results of the WUM algorithms. User testing was used to determine the effectiveness and usefulness of WebPatterns for visualising web usage patterns. The results of the user testing clearly show that the participants were highly satisfied with the visual design and information provided by WebPatterns. All the participants also indicated that they would like to use WebPatterns in the future. Analysis of the web usage patterns presented by WebPatterns was used to determine that the information architecture of the CS&IS web site can be restructured to better facilitate information retrieval. Changes to the CS&IS web site web were suggested, included placing embedded hyperlinks on the home page to the frequently accessed sections of the web site.
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- Date Issued: 2005