An exploration of factors that impact on levels of employee satisfaction and organisational performance : an organisational diagnosis
- Authors: Foot, Kirsten Joan
- Date: 2004
- Subjects: Employee motivation Job satisfaction Employees -- Rating of Psychology, Industrial Organizational change Hotels -- Employees -- South Africa -- Case studies
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3178 , http://hdl.handle.net/10962/d1007951
- Description: Organisations today, regardless of their function, exist in an environment that is characterised by change. In order to maintain a competitive advantage it is vital that organisations manage such change and are sensitive to their human resource. It is imperative for organisations to understand and explore the factors that impact on employee satisfaction and overall organisational performance. The hospitality industry is an industry that is notorious for low levels of pay and long working hours, and often dissatisfied employees. This research focused on a hotel, that is part of an international chain of hotels, which has recently undergone a rebranding process (a change from within). This research aimed to assess and explore factors that impact on levels of employee satisfaction and organisation/hotel performance, in other words it aimed to 'diagnose' the hotel's current status. The research was conducted in two phases. Phase one made use of a widely used measure of job satisfaction, the job descriptive index (JDI), that looked at five facets of job satisfaction namely: pay, opportunity for promotion, co-workers, supervision and the nature of work. Phase two further explored the results of the JDI (staff being very dissatisfied with pay and promotions opportunity) and further explored other areas of the organisation/hotel with the use of an organisational development model, Weisbord's Six-Box Model (1990). The 'boxes' included areas of purpose, structure, relationships, leadership, rewards and helpful mechanisms. These areas were explored with staff using focus groups. Heads of departments (management) and the deputy general manager of the hotel were interviewed using a semi-structured interview format, exploring similar issues to those researched with staff. Results indicated problems in the hotel with regard to purpose, a severe lack of communication and staff feeling they have little chance for promotion as well as pay structures being perceived as unfair. The overall leadership at the hotel was described as erratic, and relationships between management revealed high levels of mistrust. Due to limited research in the South African hospitality industry, much of the literature available is based on experiences in the United States of America or the United Kingdom. For this research, the researcher had few previous published findings and was unsure of the many issues that could possibly arise. However, the intervention was enjoyable and recommendations have been provided for the hotel to consider, so the hotel can go from "good to great".
- Full Text:
- Date Issued: 2004
- Authors: Foot, Kirsten Joan
- Date: 2004
- Subjects: Employee motivation Job satisfaction Employees -- Rating of Psychology, Industrial Organizational change Hotels -- Employees -- South Africa -- Case studies
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3178 , http://hdl.handle.net/10962/d1007951
- Description: Organisations today, regardless of their function, exist in an environment that is characterised by change. In order to maintain a competitive advantage it is vital that organisations manage such change and are sensitive to their human resource. It is imperative for organisations to understand and explore the factors that impact on employee satisfaction and overall organisational performance. The hospitality industry is an industry that is notorious for low levels of pay and long working hours, and often dissatisfied employees. This research focused on a hotel, that is part of an international chain of hotels, which has recently undergone a rebranding process (a change from within). This research aimed to assess and explore factors that impact on levels of employee satisfaction and organisation/hotel performance, in other words it aimed to 'diagnose' the hotel's current status. The research was conducted in two phases. Phase one made use of a widely used measure of job satisfaction, the job descriptive index (JDI), that looked at five facets of job satisfaction namely: pay, opportunity for promotion, co-workers, supervision and the nature of work. Phase two further explored the results of the JDI (staff being very dissatisfied with pay and promotions opportunity) and further explored other areas of the organisation/hotel with the use of an organisational development model, Weisbord's Six-Box Model (1990). The 'boxes' included areas of purpose, structure, relationships, leadership, rewards and helpful mechanisms. These areas were explored with staff using focus groups. Heads of departments (management) and the deputy general manager of the hotel were interviewed using a semi-structured interview format, exploring similar issues to those researched with staff. Results indicated problems in the hotel with regard to purpose, a severe lack of communication and staff feeling they have little chance for promotion as well as pay structures being perceived as unfair. The overall leadership at the hotel was described as erratic, and relationships between management revealed high levels of mistrust. Due to limited research in the South African hospitality industry, much of the literature available is based on experiences in the United States of America or the United Kingdom. For this research, the researcher had few previous published findings and was unsure of the many issues that could possibly arise. However, the intervention was enjoyable and recommendations have been provided for the hotel to consider, so the hotel can go from "good to great".
- Full Text:
- Date Issued: 2004
An exploration of how professional graphic design discourse impacts on innovation : a focus on the articulation of a South African design language in i-jusi
- Authors: Moys, Jeanne Louise
- Date: 2004
- Subjects: Graphic arts -- South Africa , Words in art , Writing in art
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3468 , http://hdl.handle.net/10962/d1002923 , Graphic arts -- South Africa , Words in art , Writing in art
- Description: This study examines the graphic design industry’s call for ‘a South African design language’ in post-apartheid South Africa and how the non-commercial publication i-jusi is envisaged as a space for graphic designers to innovate a South African design language. The central premise of this research is that graphic design, as a form of cultural production, is discursive. In this respect, graphic design practice is constructed and constrained by professional discourse, which is in turn informed by social structures. However, discourse is also a site of contestation and graphic designers may challenge or negotiate professional discourse in their practice. Thus, as Wolff (1981) argues, the possibility for innovation within graphic design practice may exist at a particular historical moment, although this possibility is itself situated within social structures. In this study, the impact of professional graphic design discourse on the attempt to innovate a South African design language in i-jusi is explored. Utilising qualitative interviews and other texts selected from graphic design commentary (conference presentations and published articles), the motivations of the producers of i-jusi are examined with a view to assessing how their articulation of a South African design language is informed by professional graphic design discourse.
- Full Text:
- Date Issued: 2004
- Authors: Moys, Jeanne Louise
- Date: 2004
- Subjects: Graphic arts -- South Africa , Words in art , Writing in art
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3468 , http://hdl.handle.net/10962/d1002923 , Graphic arts -- South Africa , Words in art , Writing in art
- Description: This study examines the graphic design industry’s call for ‘a South African design language’ in post-apartheid South Africa and how the non-commercial publication i-jusi is envisaged as a space for graphic designers to innovate a South African design language. The central premise of this research is that graphic design, as a form of cultural production, is discursive. In this respect, graphic design practice is constructed and constrained by professional discourse, which is in turn informed by social structures. However, discourse is also a site of contestation and graphic designers may challenge or negotiate professional discourse in their practice. Thus, as Wolff (1981) argues, the possibility for innovation within graphic design practice may exist at a particular historical moment, although this possibility is itself situated within social structures. In this study, the impact of professional graphic design discourse on the attempt to innovate a South African design language in i-jusi is explored. Utilising qualitative interviews and other texts selected from graphic design commentary (conference presentations and published articles), the motivations of the producers of i-jusi are examined with a view to assessing how their articulation of a South African design language is informed by professional graphic design discourse.
- Full Text:
- Date Issued: 2004
An exploration of teacher perceptions and actions to conserve wetlands in Kenya
- Authors: Ndaruga, Ayub Macharia
- Date: 2004
- Subjects: Wetlands -- Kenya Wetland conservation -- Kenya Wetland conservation -- Kenya -- Study and teaching ( Elementary) Wetlands -- Kenya -- Study and teaching (Elementary) Environmental education -- Kenya Sustainable development -- Study and teaching (Elementary) -- Kenya
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1535 , http://hdl.handle.net/10962/d1003417
- Description: This thesis presents the findings of an exploration of teacher perceptions and actions to conserve wetlands in Kenya. It reports findings of a case study survey done with 54 primary school teachers from seven of eight Kenyan provinces. The teachers were sampled from a larger group of 242 teachers who participated in an in-service course on wetlands conservation. The in-service course engaged teachers in exploring various aspects of wetlands and how they could enhance their conservation using both formal and non-formal contexts. The survey approach was used in the research and was augmented with a reflective process. Survey tools used were the questionnaires and interviews. The reflective process entailed observation, document analysis, field notes and the researcher’s diary. Research data was analysed in several stages. The study revealed that teachers were users of local wetlands just like other members of their communities. The teachers differed among themselves with respect to their perceptions of the value and threats to their local wetlands. The teachers also differed in their perceptions of community awareness of their local wetlands. The teachers’ perceptions about environmental education and wetlands were not holistic. The teachers recorded various opportunities to foster wetlands conservation in their local contexts at school and the community. These were their fellow teachers, the pupils, the subjects taught, clubs, environmental days and the community. The relative importance of these opportunities differed among the teachers. Many teachers claimed being motivated to promote wetlands conservation using both formal and non-formal contexts. Their motivation was based on diverse aspects of wetlands, education and the in-service training they attended in 1999. However, the intensity of motivation differed for each aspect. For instance, at community level in-service training was a major motivator while the curriculum aspects were not mentioned at all. Teachers reported having involved their pupils and the community in several activities to conserve the local wetlands. These activities seemed to be dominated by theoretical approaches, eco-management activities and visits to wetlands. There was little evidence to indicate the teachers being engaged in addressing the implications for sustainability of the perceived local wetland values and threats. The activities reported also failed to show engagement with local environmental problems as a deliberate proactive process involving exploration, discussion, action taking and reflection. Most of the activities were presented as one off activities rather than as a cyclic continuously improving series of interventions. The overall scenario presented by the teachers is one of inadequacy in addressing the sustainable use of wetlands. Teachers suggested various constraints that affect their realisation of environmental education for wetlands sustainability. The constraints traverse various aspects of the economic, biophysical, political, educational and social aspects of wetlands conservation. The study suggested the need for teachers to treat their contextual and personal conceptions as problematic rather than as simplistic or linear issues and to formulate ways to address them. This study suggests a potential but under-utilised opportunity to promote wetlands sustainability. The overall picture generated by the data in this study is a need to consider wetland training for teachers that integrates the holistic aspects of wetlands as well as the environmental education for sustainability perspectives grounded in local contexts.
- Full Text:
- Date Issued: 2004
- Authors: Ndaruga, Ayub Macharia
- Date: 2004
- Subjects: Wetlands -- Kenya Wetland conservation -- Kenya Wetland conservation -- Kenya -- Study and teaching ( Elementary) Wetlands -- Kenya -- Study and teaching (Elementary) Environmental education -- Kenya Sustainable development -- Study and teaching (Elementary) -- Kenya
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1535 , http://hdl.handle.net/10962/d1003417
- Description: This thesis presents the findings of an exploration of teacher perceptions and actions to conserve wetlands in Kenya. It reports findings of a case study survey done with 54 primary school teachers from seven of eight Kenyan provinces. The teachers were sampled from a larger group of 242 teachers who participated in an in-service course on wetlands conservation. The in-service course engaged teachers in exploring various aspects of wetlands and how they could enhance their conservation using both formal and non-formal contexts. The survey approach was used in the research and was augmented with a reflective process. Survey tools used were the questionnaires and interviews. The reflective process entailed observation, document analysis, field notes and the researcher’s diary. Research data was analysed in several stages. The study revealed that teachers were users of local wetlands just like other members of their communities. The teachers differed among themselves with respect to their perceptions of the value and threats to their local wetlands. The teachers also differed in their perceptions of community awareness of their local wetlands. The teachers’ perceptions about environmental education and wetlands were not holistic. The teachers recorded various opportunities to foster wetlands conservation in their local contexts at school and the community. These were their fellow teachers, the pupils, the subjects taught, clubs, environmental days and the community. The relative importance of these opportunities differed among the teachers. Many teachers claimed being motivated to promote wetlands conservation using both formal and non-formal contexts. Their motivation was based on diverse aspects of wetlands, education and the in-service training they attended in 1999. However, the intensity of motivation differed for each aspect. For instance, at community level in-service training was a major motivator while the curriculum aspects were not mentioned at all. Teachers reported having involved their pupils and the community in several activities to conserve the local wetlands. These activities seemed to be dominated by theoretical approaches, eco-management activities and visits to wetlands. There was little evidence to indicate the teachers being engaged in addressing the implications for sustainability of the perceived local wetland values and threats. The activities reported also failed to show engagement with local environmental problems as a deliberate proactive process involving exploration, discussion, action taking and reflection. Most of the activities were presented as one off activities rather than as a cyclic continuously improving series of interventions. The overall scenario presented by the teachers is one of inadequacy in addressing the sustainable use of wetlands. Teachers suggested various constraints that affect their realisation of environmental education for wetlands sustainability. The constraints traverse various aspects of the economic, biophysical, political, educational and social aspects of wetlands conservation. The study suggested the need for teachers to treat their contextual and personal conceptions as problematic rather than as simplistic or linear issues and to formulate ways to address them. This study suggests a potential but under-utilised opportunity to promote wetlands sustainability. The overall picture generated by the data in this study is a need to consider wetland training for teachers that integrates the holistic aspects of wetlands as well as the environmental education for sustainability perspectives grounded in local contexts.
- Full Text:
- Date Issued: 2004
An exploration of the impact of AIDS-related losses and role changes on grandmothers
- Authors: Burt, Mary
- Date: 2004
- Subjects: HIV infections -- Social aspects -- South Africa -- Eastern Cape HIV infections -- Economic aspects -- South Africa -- Eastern Cape AIDS (Disease) -- Social aspects -- South Africa -- Eastern Cape AIDS (Disease) -- Economic aspects -- South Africa -- Eastern Cape AIDS (Disease) -- Patients -- Care -- South Africa -- Eastern Cape Bereavement -- Psychological aspects -- Eastern Cape Foster parents -- South Africa -- Eastern Cape Loss (Psychology) Grandmothers -- South Africa -- Psychology
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3131 , http://hdl.handle.net/10962/d1006487
- Description: The US Bureau of the Census (1999) projected that by 2004, 14 million people in sub-Saharan Africa will develop full blown AIDS, making this region by far the largest disease burden in the world (World Health Organization, 2002). The United Nations AIDS Programme judged South Africa to have the leading number of people living with HIV/AIDS worldwide (World Health Organisation, 2002). To date there has been extensive research conducted on the socio-economic impacts of HIV/AIDS on families in Africa. However an area of investigation that has remained largely underreported is the inquiry into the psychological impacts of HIV/AIDS on elderly caregivers. In African families older women increasingly have to provide care to their adult children with AIDS and their orphaned grandchildren. However few research studies have assessed the experience of parental caregiving and its psychological impacts on these women. This qualitative research study hypothesised that the role of primary parental caregiver in fact causes a range of psychologically distressing states, which serve to compromise the psychological well-being of these caregivers. To investigate this hypothesis three Xhosa speaking women living in informal settlements in Grahamstown, in the Eastern Cape Province of South Africa were selected for the study. The women were interviewed by means of semi-structured interviews, which consisted of questions related to their caregiving experiences, their experiences of loss, their choice of coping strategies, the role of support networks and their experiences of foster care responsibilities. The interviews were transcribed and analysed using a grounded hermeneutic approach. The research results confirmed the working hypothesis. The research revealed that although it was considered culturally appropriate for older women to care for their children and grandchildren, their caregiver roles caused significant psychological distress. Their distress was related to: emotional and physical exhaustion, complicated grief reactions and ongoing emotional and physical upheaval related to foster care responsibilities. Based on the results, the research recommendations emphasised the need for continual awareness of the psychological implications of caregiving for older African women with the aim to preserve their capacity to function as the primary caring resource to families struck by HIV/AIDS.
- Full Text:
- Date Issued: 2004
- Authors: Burt, Mary
- Date: 2004
- Subjects: HIV infections -- Social aspects -- South Africa -- Eastern Cape HIV infections -- Economic aspects -- South Africa -- Eastern Cape AIDS (Disease) -- Social aspects -- South Africa -- Eastern Cape AIDS (Disease) -- Economic aspects -- South Africa -- Eastern Cape AIDS (Disease) -- Patients -- Care -- South Africa -- Eastern Cape Bereavement -- Psychological aspects -- Eastern Cape Foster parents -- South Africa -- Eastern Cape Loss (Psychology) Grandmothers -- South Africa -- Psychology
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3131 , http://hdl.handle.net/10962/d1006487
- Description: The US Bureau of the Census (1999) projected that by 2004, 14 million people in sub-Saharan Africa will develop full blown AIDS, making this region by far the largest disease burden in the world (World Health Organization, 2002). The United Nations AIDS Programme judged South Africa to have the leading number of people living with HIV/AIDS worldwide (World Health Organisation, 2002). To date there has been extensive research conducted on the socio-economic impacts of HIV/AIDS on families in Africa. However an area of investigation that has remained largely underreported is the inquiry into the psychological impacts of HIV/AIDS on elderly caregivers. In African families older women increasingly have to provide care to their adult children with AIDS and their orphaned grandchildren. However few research studies have assessed the experience of parental caregiving and its psychological impacts on these women. This qualitative research study hypothesised that the role of primary parental caregiver in fact causes a range of psychologically distressing states, which serve to compromise the psychological well-being of these caregivers. To investigate this hypothesis three Xhosa speaking women living in informal settlements in Grahamstown, in the Eastern Cape Province of South Africa were selected for the study. The women were interviewed by means of semi-structured interviews, which consisted of questions related to their caregiving experiences, their experiences of loss, their choice of coping strategies, the role of support networks and their experiences of foster care responsibilities. The interviews were transcribed and analysed using a grounded hermeneutic approach. The research results confirmed the working hypothesis. The research revealed that although it was considered culturally appropriate for older women to care for their children and grandchildren, their caregiver roles caused significant psychological distress. Their distress was related to: emotional and physical exhaustion, complicated grief reactions and ongoing emotional and physical upheaval related to foster care responsibilities. Based on the results, the research recommendations emphasised the need for continual awareness of the psychological implications of caregiving for older African women with the aim to preserve their capacity to function as the primary caring resource to families struck by HIV/AIDS.
- Full Text:
- Date Issued: 2004
An interpretive use of drawings to explore the lived experiences of orphaned children living with HIV/AIDS in South Africa
- Authors: Steenveld, Clint Michael
- Date: 2004
- Subjects: AIDS (Disease) -- South Africa AIDS (Disease) -- Social aspects -- South Africa HIV infections -- South Africa HIV infections -- Social aspects -- South Africa AIDS (Disease) -- Psychological aspects HIV infections -- Psychological aspects -- South Africa AIDS (Disease) in children -- Psychological aspects -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3099 , http://hdl.handle.net/10962/d1004374
- Description: Against the backdrop of the growing problem of AIDS orphans in South Africa and greater sub-Saharan Africa, this qualitative enquiry examines the lives of three South African orphaned children living with HIV / AIDS in a children's home in Cape Town. It aims to generate rich, child-centred descriptions of some of the significant experiences of the children's lives. Drawings, dialogue and narrative were employed to generate the primary data. This was supplemented by collateral interviews and other relevant records, e.g. medical and biographical. Existential-phenomenological theory informed the approach to data collection and analysis. Each child produced a series often to twelve impromptu drawings over a period often weeks. These drawings and transcripts of the children's verbal descriptions of their drawings were extensively analysed. Significant themes for each participant as well as themes common to all three were identified. Some of the central themes emerging include loss, abandonment, death, disease awareness and coping. The children's ability to develop adaptive coping mechanisms and resilience in the face of traumatic loss and terminal illness was a particularly outstanding feature of the findings. Recommendations are made regarding future research to address the lack of qualitative, child-focused investigations as well as appropriate interventions for addressing the psychosocial needs of orphaned children living with HIV/AIDS.
- Full Text:
- Date Issued: 2004
- Authors: Steenveld, Clint Michael
- Date: 2004
- Subjects: AIDS (Disease) -- South Africa AIDS (Disease) -- Social aspects -- South Africa HIV infections -- South Africa HIV infections -- Social aspects -- South Africa AIDS (Disease) -- Psychological aspects HIV infections -- Psychological aspects -- South Africa AIDS (Disease) in children -- Psychological aspects -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3099 , http://hdl.handle.net/10962/d1004374
- Description: Against the backdrop of the growing problem of AIDS orphans in South Africa and greater sub-Saharan Africa, this qualitative enquiry examines the lives of three South African orphaned children living with HIV / AIDS in a children's home in Cape Town. It aims to generate rich, child-centred descriptions of some of the significant experiences of the children's lives. Drawings, dialogue and narrative were employed to generate the primary data. This was supplemented by collateral interviews and other relevant records, e.g. medical and biographical. Existential-phenomenological theory informed the approach to data collection and analysis. Each child produced a series often to twelve impromptu drawings over a period often weeks. These drawings and transcripts of the children's verbal descriptions of their drawings were extensively analysed. Significant themes for each participant as well as themes common to all three were identified. Some of the central themes emerging include loss, abandonment, death, disease awareness and coping. The children's ability to develop adaptive coping mechanisms and resilience in the face of traumatic loss and terminal illness was a particularly outstanding feature of the findings. Recommendations are made regarding future research to address the lack of qualitative, child-focused investigations as well as appropriate interventions for addressing the psychosocial needs of orphaned children living with HIV/AIDS.
- Full Text:
- Date Issued: 2004
An investigation into how marketers cope with an environment of high complexity and turbulence, with special reference to the South African environment
- Authors: Mason, Roger Bruce
- Date: 2004
- Subjects: Marketing -- South Africa , Success in business -- South Africa , Industrial management -- South Africa , Organizational change -- South Africa , Chaotic behavior in systems , Complexity (Philosophy)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:752 , http://hdl.handle.net/10962/d1003873 , Marketing -- South Africa , Success in business -- South Africa , Industrial management -- South Africa , Organizational change -- South Africa , Chaotic behavior in systems , Complexity (Philosophy)
- Description: This study investigated relationships between marketing success, the level of external environmental complexity and turbulence, and marketing mix tactics. The literature suggested that more successful companies in simple and stable environments use stabilising marketing tactics, while more successful companies in complex and turbulent environments use destabilising tactics, regardless of industry habits, management preferences or market sector. A marketing mix model for the different environments was developed using a chaos and complexity theory perspective. The study was exploratory, using a qualitative, case study technique. Data was collected via depth interviews and document analysis from four companies in the information technology (IT) and packaging industries. These industries were identified as, respectively, the most complex and turbulent, and the simplest and most stable, South African environments. Two companies from each industry were chosen to reflect more successful and less successful companies. The more successful company in the complex/turbulent environment was found to use destabilising tactics, as did the more successful company in the simple/stable environment. Therefore, contrary to expectations, it appears that destabilising tactics contribute more to success than stabilising tactics do, regardless of the environment. It was also found, contrary to expectations, that stabilising tactics were used by both the less successful companies. The research concluded that destabilising tactics are related to more success and stabilising tactics to less success. The lack of clear differentiation between the two industries may be because the whole South African environment is complex and turbulent, because the packaging industry is not sufficiently simple and stable to differentiate it from the IT industry or because packaging industry managers perceive their industry to be complex and turbulent and act accordingly. Despite these uncertainties, the research showed the marketing mix model to be reasonably accurate for the complex/turbulent environment, and therefore of potential value to South African companies. To overcome the equivocal findings, further research is recommended in different industries, in countries with different levels of complexity and turbulence and into specific marketing mix tactics. Research into managers’ perceptions of environmental complexity and turbulence and into co-evolution of marketing tactics and external environments would also be of value.
- Full Text:
- Date Issued: 2004
- Authors: Mason, Roger Bruce
- Date: 2004
- Subjects: Marketing -- South Africa , Success in business -- South Africa , Industrial management -- South Africa , Organizational change -- South Africa , Chaotic behavior in systems , Complexity (Philosophy)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:752 , http://hdl.handle.net/10962/d1003873 , Marketing -- South Africa , Success in business -- South Africa , Industrial management -- South Africa , Organizational change -- South Africa , Chaotic behavior in systems , Complexity (Philosophy)
- Description: This study investigated relationships between marketing success, the level of external environmental complexity and turbulence, and marketing mix tactics. The literature suggested that more successful companies in simple and stable environments use stabilising marketing tactics, while more successful companies in complex and turbulent environments use destabilising tactics, regardless of industry habits, management preferences or market sector. A marketing mix model for the different environments was developed using a chaos and complexity theory perspective. The study was exploratory, using a qualitative, case study technique. Data was collected via depth interviews and document analysis from four companies in the information technology (IT) and packaging industries. These industries were identified as, respectively, the most complex and turbulent, and the simplest and most stable, South African environments. Two companies from each industry were chosen to reflect more successful and less successful companies. The more successful company in the complex/turbulent environment was found to use destabilising tactics, as did the more successful company in the simple/stable environment. Therefore, contrary to expectations, it appears that destabilising tactics contribute more to success than stabilising tactics do, regardless of the environment. It was also found, contrary to expectations, that stabilising tactics were used by both the less successful companies. The research concluded that destabilising tactics are related to more success and stabilising tactics to less success. The lack of clear differentiation between the two industries may be because the whole South African environment is complex and turbulent, because the packaging industry is not sufficiently simple and stable to differentiate it from the IT industry or because packaging industry managers perceive their industry to be complex and turbulent and act accordingly. Despite these uncertainties, the research showed the marketing mix model to be reasonably accurate for the complex/turbulent environment, and therefore of potential value to South African companies. To overcome the equivocal findings, further research is recommended in different industries, in countries with different levels of complexity and turbulence and into specific marketing mix tactics. Research into managers’ perceptions of environmental complexity and turbulence and into co-evolution of marketing tactics and external environments would also be of value.
- Full Text:
- Date Issued: 2004
An investigation into school learners' perceptions of linguistic politeness norms within and across cultures
- Authors: Kenyon, Tracy Karen
- Date: 2004 , 2013-05-31
- Subjects: Language policy -- South Africa , Multilingualism -- South Africa -- Eastern Cape , Language and education -- South Africa -- Eastern Cape , Language and culture -- South Africa -- Eastern Cape , Multiculturalism -- South Africa -- Eastern Cape , Social interaction -- South Africa -- Eastern Cape , Sociolinguistics -- South Africa -- Eastern Cape , Intercultural communication -- South Africa -- Eastern Cape , Speech acts (Linguistics)
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2371 , http://hdl.handle.net/10962/d1004715 , Language policy -- South Africa , Multilingualism -- South Africa -- Eastern Cape , Language and education -- South Africa -- Eastern Cape , Language and culture -- South Africa -- Eastern Cape , Multiculturalism -- South Africa -- Eastern Cape , Social interaction -- South Africa -- Eastern Cape , Sociolinguistics -- South Africa -- Eastern Cape , Intercultural communication -- South Africa -- Eastern Cape , Speech acts (Linguistics)
- Description: The assumption underlying this study is that cultures differ in terms of politeness norms. Often people from different cultures approach one another in what they think is an appropriate manner and the outcome is miscommunication. This may be attributed to differing cultural norms and this study aims to examine what a sample of school learners perceive to be polite behaviour when making requests and their reasons for doing so. This study focuses on perceived politeness norms (Brown and Levinson 1978) in English across selected South African cultures. The individuals are seen as reflecting a cultural identity, using norms that they feel are appropriate in given situations. Previous researchers have endeavoured to show that politeness norms are universal, but it has emerged that this is not always the case. When people from differing language and cultural backgrounds come into contact they have to find a common ground for their interaction to be successful (Lustig and Koester 1999). Of particular interest is the way people request things, both the way they phrase their request and their reasons for phrasing it this way. In order to investigate this, twenty-nine same-sex pairs of Grade 10 learners were selected from three schools with different cultural backgrounds in Grahamstown. These learners were required to complete a Discourse Completion Test, which contained both Think-Aloud and Retrospection Procedures, while they were being audio-taped. This data was transcribed and analysed using a model that was developed and adapted to describe request strategies. This data is shown through the use of basic statistics, even though it is primarily qualitative. The data is given this qualitative dimension by looking at the factors that the co-conversants attend to. The recorded data shows that although second language speakers of English have a formula for requesting things, they are not always able to articulate why they use the request strategies they do. It appears that English first language speakers and speakers who have English as an additional language request things similarly, but the first language speakers have access to a greater variety of politeness strategies. They also attend to different contextual features. This shows that while the need to be polite seems to be universal, the expectations of the speakers will be different and while a first language speaker of English would not misinterpret the force of a given speech act, they may feel that the person who has English as an additional language is rude. Sensitivity is therefore called for in order to combat mutual negative stereotyping and misunderstandings. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2004
- Authors: Kenyon, Tracy Karen
- Date: 2004 , 2013-05-31
- Subjects: Language policy -- South Africa , Multilingualism -- South Africa -- Eastern Cape , Language and education -- South Africa -- Eastern Cape , Language and culture -- South Africa -- Eastern Cape , Multiculturalism -- South Africa -- Eastern Cape , Social interaction -- South Africa -- Eastern Cape , Sociolinguistics -- South Africa -- Eastern Cape , Intercultural communication -- South Africa -- Eastern Cape , Speech acts (Linguistics)
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2371 , http://hdl.handle.net/10962/d1004715 , Language policy -- South Africa , Multilingualism -- South Africa -- Eastern Cape , Language and education -- South Africa -- Eastern Cape , Language and culture -- South Africa -- Eastern Cape , Multiculturalism -- South Africa -- Eastern Cape , Social interaction -- South Africa -- Eastern Cape , Sociolinguistics -- South Africa -- Eastern Cape , Intercultural communication -- South Africa -- Eastern Cape , Speech acts (Linguistics)
- Description: The assumption underlying this study is that cultures differ in terms of politeness norms. Often people from different cultures approach one another in what they think is an appropriate manner and the outcome is miscommunication. This may be attributed to differing cultural norms and this study aims to examine what a sample of school learners perceive to be polite behaviour when making requests and their reasons for doing so. This study focuses on perceived politeness norms (Brown and Levinson 1978) in English across selected South African cultures. The individuals are seen as reflecting a cultural identity, using norms that they feel are appropriate in given situations. Previous researchers have endeavoured to show that politeness norms are universal, but it has emerged that this is not always the case. When people from differing language and cultural backgrounds come into contact they have to find a common ground for their interaction to be successful (Lustig and Koester 1999). Of particular interest is the way people request things, both the way they phrase their request and their reasons for phrasing it this way. In order to investigate this, twenty-nine same-sex pairs of Grade 10 learners were selected from three schools with different cultural backgrounds in Grahamstown. These learners were required to complete a Discourse Completion Test, which contained both Think-Aloud and Retrospection Procedures, while they were being audio-taped. This data was transcribed and analysed using a model that was developed and adapted to describe request strategies. This data is shown through the use of basic statistics, even though it is primarily qualitative. The data is given this qualitative dimension by looking at the factors that the co-conversants attend to. The recorded data shows that although second language speakers of English have a formula for requesting things, they are not always able to articulate why they use the request strategies they do. It appears that English first language speakers and speakers who have English as an additional language request things similarly, but the first language speakers have access to a greater variety of politeness strategies. They also attend to different contextual features. This shows that while the need to be polite seems to be universal, the expectations of the speakers will be different and while a first language speaker of English would not misinterpret the force of a given speech act, they may feel that the person who has English as an additional language is rude. Sensitivity is therefore called for in order to combat mutual negative stereotyping and misunderstandings. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2004
An investigation into school principals' experience and perceptions of participative management
- Authors: Mungunda, Charles
- Date: 2004
- Subjects: Education -- Namibia Educational change -- Namibia School management and organization -- Namibia Educational leadership -- Namibia Management -- Employee participation -- Namibia
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1599 , http://hdl.handle.net/10962/d1003481
- Description: The purpose of this research was to investigate three principals’ perceptions and experience of participative management and to gain insight into and understanding of the dimensions involved in participative management from the perspective of the three principals. The research was conducted within the interpretive paradigm, since I was interested in understanding the subjective experience and individual perceptions of three principals with regard to participative management and the meaning they attach to it in their natural settings: how they describe, interpret and make sense of participative management. Data were collected by means of questionnaires, interviews and observation. The findings indicate overwhelming support for the notion that participative management (collegiality) does have a positive influence with regard to creating a sense of common goals, shared vision, a sense of ownership, commitment and improved human relations. The respondents emphasized the critical role of the leader in facilitating the implementation of collegiality as well as an increasing awareness of the potential of participative management to improve human development through delegation. The responses revealed interesting paradoxes with regard to the tensions between leadership and leaderless organizations, as well as between autonomy and interdependence. Responses also reflect the sentiment that participative management should be selective, involving only those with the necessary skills and expertise in the decision-making process. While there is an appreciation of the benefits to be derived from collegiality, critical statements about participative management include the time-consuming nature of participative management, and the notion that participative management impinges on the authority of the principals.
- Full Text:
- Date Issued: 2004
- Authors: Mungunda, Charles
- Date: 2004
- Subjects: Education -- Namibia Educational change -- Namibia School management and organization -- Namibia Educational leadership -- Namibia Management -- Employee participation -- Namibia
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1599 , http://hdl.handle.net/10962/d1003481
- Description: The purpose of this research was to investigate three principals’ perceptions and experience of participative management and to gain insight into and understanding of the dimensions involved in participative management from the perspective of the three principals. The research was conducted within the interpretive paradigm, since I was interested in understanding the subjective experience and individual perceptions of three principals with regard to participative management and the meaning they attach to it in their natural settings: how they describe, interpret and make sense of participative management. Data were collected by means of questionnaires, interviews and observation. The findings indicate overwhelming support for the notion that participative management (collegiality) does have a positive influence with regard to creating a sense of common goals, shared vision, a sense of ownership, commitment and improved human relations. The respondents emphasized the critical role of the leader in facilitating the implementation of collegiality as well as an increasing awareness of the potential of participative management to improve human development through delegation. The responses revealed interesting paradoxes with regard to the tensions between leadership and leaderless organizations, as well as between autonomy and interdependence. Responses also reflect the sentiment that participative management should be selective, involving only those with the necessary skills and expertise in the decision-making process. While there is an appreciation of the benefits to be derived from collegiality, critical statements about participative management include the time-consuming nature of participative management, and the notion that participative management impinges on the authority of the principals.
- Full Text:
- Date Issued: 2004
An investigation into the captive spawning, egg characteristics and egg quality of the mud crab (Scylla serrata) in South Africa
- Authors: Churchill, Giles John
- Date: 2004
- Subjects: Scylla serrata , Crabs -- South Africa , Scylla serrata -- South Africa , Crabs -- Eggs -- Incubation , Crabs -- Spawning
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5376 , http://hdl.handle.net/10962/d1015963
- Description: The source of a reliable supply of seed is one of the most important factors determining the success of aquaculture. At present mud crab culture is reliant on wild caught juveniles, which limits the expansion of mud crab farming globally. This, coupled with the paucity of knowledge of the spawning and egg characteristics of mud crabs, prompted the investigation into the captive spawning, incubation and egg quality of Scylla serrata in South Africa. A total of 112 mature females were caught from three estuaries in northern KwaZulu Natal and acclimated to captive conditions. Mature females were present in the estuaries throughout the year and ranged from 90 to 200 mm carapace width. Except for 40 crabs all the females spawned in captivity. On average spawning took place 38 ± 23 days after capture but was not dependent on crab size. Fecundity was significantly correlated to crab size (p = 0.026), with larger females producing more eggs. The average fecundity per batch was 5.79 ± 2.07 million eggs. Hatch success rate for all egg batches was high averaging 84 ± 6 %. Egg dropping occurred in all egg batches during incubation. On average 6.5 % of the egg mass was dropped during incubation. At the average temperature of 27⁰C eggs in the incubation system hatched within 292 ± 12 hours after extrusion. In-vitro incubation of eggs proved to be an ineffective means of incubating Scylla serrata eggs with a hatch success rate of only 25 ± 5 %. Egg quality was assessed both directly, using variables such as hatch success rate and hatch synchronicity and retrospectively by using stress tests and comparing survival of larvae from different coloured egg batches. Lipid class composition and fatty acid methyl ester content for 28 batches of eggs and 16 batches of larvae were analyzed. The biochemical composition of the egg was correlated to survival of larvae in the stress tests to ascertain if the lipid content of the eggs determines their quality. Stress tests were used to retrospectively assess egg quality. Four stressors were administered to newly hatched larvae and the LD50 values recorded. An average LD50 of 64 hours was recorded for the starvation tests, 40% for the salinity tests, 37 ppm for the formalin tests and 39.7 mg/l NH3 for the ammonia stress tests was recorded. Egg colour ranged from pale yellow to orange-red. Egg colour was not influenced by female size or the time the females spent in captivity prior to spawning. Egg colour can therefore not be used as an indicator of quality. The lipid profiles of newly extruded eggs had no effect on hatch synchronicity or hatch success rate. However, the stress tests identified female size (p ≤ 0.02), DHA content of the egg (p ≤ 0.02), Σ omega-3 fatty acids of eggs (p ≤ 0.02) and EPA content of eggs (p ≤ 0.007) as possible determinants of egg quality. Larger crabs tended to produce poorer quality eggs. Egg quality also decreased as the amount of DHA, EPA and the Σ omega-3 fatty acids decreased in the eggs. To summarize, the results of this study indicated that the following parameters affect egg quality - crab size, DHA, EPA and Σ omega-3 fatty acid content of eggs.
- Full Text:
- Date Issued: 2004
- Authors: Churchill, Giles John
- Date: 2004
- Subjects: Scylla serrata , Crabs -- South Africa , Scylla serrata -- South Africa , Crabs -- Eggs -- Incubation , Crabs -- Spawning
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5376 , http://hdl.handle.net/10962/d1015963
- Description: The source of a reliable supply of seed is one of the most important factors determining the success of aquaculture. At present mud crab culture is reliant on wild caught juveniles, which limits the expansion of mud crab farming globally. This, coupled with the paucity of knowledge of the spawning and egg characteristics of mud crabs, prompted the investigation into the captive spawning, incubation and egg quality of Scylla serrata in South Africa. A total of 112 mature females were caught from three estuaries in northern KwaZulu Natal and acclimated to captive conditions. Mature females were present in the estuaries throughout the year and ranged from 90 to 200 mm carapace width. Except for 40 crabs all the females spawned in captivity. On average spawning took place 38 ± 23 days after capture but was not dependent on crab size. Fecundity was significantly correlated to crab size (p = 0.026), with larger females producing more eggs. The average fecundity per batch was 5.79 ± 2.07 million eggs. Hatch success rate for all egg batches was high averaging 84 ± 6 %. Egg dropping occurred in all egg batches during incubation. On average 6.5 % of the egg mass was dropped during incubation. At the average temperature of 27⁰C eggs in the incubation system hatched within 292 ± 12 hours after extrusion. In-vitro incubation of eggs proved to be an ineffective means of incubating Scylla serrata eggs with a hatch success rate of only 25 ± 5 %. Egg quality was assessed both directly, using variables such as hatch success rate and hatch synchronicity and retrospectively by using stress tests and comparing survival of larvae from different coloured egg batches. Lipid class composition and fatty acid methyl ester content for 28 batches of eggs and 16 batches of larvae were analyzed. The biochemical composition of the egg was correlated to survival of larvae in the stress tests to ascertain if the lipid content of the eggs determines their quality. Stress tests were used to retrospectively assess egg quality. Four stressors were administered to newly hatched larvae and the LD50 values recorded. An average LD50 of 64 hours was recorded for the starvation tests, 40% for the salinity tests, 37 ppm for the formalin tests and 39.7 mg/l NH3 for the ammonia stress tests was recorded. Egg colour ranged from pale yellow to orange-red. Egg colour was not influenced by female size or the time the females spent in captivity prior to spawning. Egg colour can therefore not be used as an indicator of quality. The lipid profiles of newly extruded eggs had no effect on hatch synchronicity or hatch success rate. However, the stress tests identified female size (p ≤ 0.02), DHA content of the egg (p ≤ 0.02), Σ omega-3 fatty acids of eggs (p ≤ 0.02) and EPA content of eggs (p ≤ 0.007) as possible determinants of egg quality. Larger crabs tended to produce poorer quality eggs. Egg quality also decreased as the amount of DHA, EPA and the Σ omega-3 fatty acids decreased in the eggs. To summarize, the results of this study indicated that the following parameters affect egg quality - crab size, DHA, EPA and Σ omega-3 fatty acid content of eggs.
- Full Text:
- Date Issued: 2004
An investigation into the neuroprotective effects of melatonin in a model of rotenone-induced neurodegeneration
- Authors: Kadanthode, Rubina John
- Date: 2004
- Subjects: Melatonin , Nervous system -- Degeneration -- Treatment , Rotenone
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3763 , http://hdl.handle.net/10962/d1003241 , Melatonin , Nervous system -- Degeneration -- Treatment , Rotenone
- Description: Parkinson’s disease, one of the most common neurodegenerative disorders associated with ageing, is characterised by abnormal and profound loss of nigrostriatal dopaminergic neurons. The cause of Parkinson’s disease is unknown, but epidemiological studies suggest an association with pesticides and other environmental toxins, and biochemical studies implicate oxidative damage and mitochondrial impairment, particularly at the level of complex I enzyme. Recently, rotenone, a commonly used organic pesticide and a classical inhibitor of mitochondrial complex I has been reported to reproduce the specific features of Parkinson’s disease in rodents. The mitochondrial respiratory chain is one of the most important sites of reactive oxygen species production under physiological conditions. Toxic free radicals have been implicated in a variety of neurodegenerative diseases as well as ageing itself. Melatonin, a secretory product of the pineal gland is a multifaceted free radical scavenger and natural antioxidant. In the present study, the neuroprotective effects of melatonin against the environmental neurotoxin, rotenone was investigated. Initial studies showed that inhibition of mitochondrial complex I enzyme by rotenone induced superoxide radical generation. Melatonin, administered to the rat in vivo and in vitro was able to offer neuroprotection by curtailing the production of superoxide radicals induced by rotenone. Mitochondria, being the major target of rotenone, the effects of melatonin were investigated at the mitochondrial level. Melatonin was able to increase the electron transport chain activity thus preventing the respiratory inhibition by rotenone. The pineal hormone also counteracted the action of rotenone on complex I enzyme. These results suggest melatonin’s ability to potentially limit the free radical generation and thereby modulate the mitochondrial functions. The detection and measurement of lipid peroxidation is the evidence most frequently cited to support the involvement of free radical reactions in toxicology and in human disease. Melatonin also offered significant protection in vivo and in vitro against rotenone induced lipid peroxidation. Since iron plays a major role in oxidative damage and in the progression of Parkinson’s disease, the effect of melatonin on both rotenone and iron induced lipid peroxidation was investigated, the results of which show that melatonin affords protection and this was suggested to be due to its interaction with the rotenone-iron complex that might have formed. Electrochemical studies were further used to characterise the interactions between melatonin, rotenone and iron (III). Melatonin was shown to bind with iron and thus reducing their toxicity. Histological studies were undertaken to assess the effects of melatonin on rotenone induced toxicity on the dopaminergic neurons in the rat brain. Rotenone treated brains showed extensive neuronal damage whereas with melatonin less damage was observed. Rotenone induces apoptosis via reactive oxygen species production and apoptotic cell death has been identified in PD brains. Furthermore, the apoptotic cell death was detected and quantified by the TUNEL staining. Rotenone treated sections showed signs of apoptosis whereas with melatonin, less apoptotic damage was observed. The findings of this study indicate that the neurohormone, melatonin may protect against rotenone-induced neurodegeneration. Since melatonin production falls substantially during ageing, the loss of this antioxidant is theorized to be instrumental in the degenerative processes associated with advanced age. Considering how devastating diseases such as Parkinson’s disease, are to a patient and the patient’s families, the discovery of protective agents are a matter of urgency. Further investigations using the pesticide model will help to determine the involvement of environmental exposure in the pathogenesis of human diseases as well as to test therapeutic strategies for the treatment of such diseases.
- Full Text:
- Date Issued: 2004
- Authors: Kadanthode, Rubina John
- Date: 2004
- Subjects: Melatonin , Nervous system -- Degeneration -- Treatment , Rotenone
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3763 , http://hdl.handle.net/10962/d1003241 , Melatonin , Nervous system -- Degeneration -- Treatment , Rotenone
- Description: Parkinson’s disease, one of the most common neurodegenerative disorders associated with ageing, is characterised by abnormal and profound loss of nigrostriatal dopaminergic neurons. The cause of Parkinson’s disease is unknown, but epidemiological studies suggest an association with pesticides and other environmental toxins, and biochemical studies implicate oxidative damage and mitochondrial impairment, particularly at the level of complex I enzyme. Recently, rotenone, a commonly used organic pesticide and a classical inhibitor of mitochondrial complex I has been reported to reproduce the specific features of Parkinson’s disease in rodents. The mitochondrial respiratory chain is one of the most important sites of reactive oxygen species production under physiological conditions. Toxic free radicals have been implicated in a variety of neurodegenerative diseases as well as ageing itself. Melatonin, a secretory product of the pineal gland is a multifaceted free radical scavenger and natural antioxidant. In the present study, the neuroprotective effects of melatonin against the environmental neurotoxin, rotenone was investigated. Initial studies showed that inhibition of mitochondrial complex I enzyme by rotenone induced superoxide radical generation. Melatonin, administered to the rat in vivo and in vitro was able to offer neuroprotection by curtailing the production of superoxide radicals induced by rotenone. Mitochondria, being the major target of rotenone, the effects of melatonin were investigated at the mitochondrial level. Melatonin was able to increase the electron transport chain activity thus preventing the respiratory inhibition by rotenone. The pineal hormone also counteracted the action of rotenone on complex I enzyme. These results suggest melatonin’s ability to potentially limit the free radical generation and thereby modulate the mitochondrial functions. The detection and measurement of lipid peroxidation is the evidence most frequently cited to support the involvement of free radical reactions in toxicology and in human disease. Melatonin also offered significant protection in vivo and in vitro against rotenone induced lipid peroxidation. Since iron plays a major role in oxidative damage and in the progression of Parkinson’s disease, the effect of melatonin on both rotenone and iron induced lipid peroxidation was investigated, the results of which show that melatonin affords protection and this was suggested to be due to its interaction with the rotenone-iron complex that might have formed. Electrochemical studies were further used to characterise the interactions between melatonin, rotenone and iron (III). Melatonin was shown to bind with iron and thus reducing their toxicity. Histological studies were undertaken to assess the effects of melatonin on rotenone induced toxicity on the dopaminergic neurons in the rat brain. Rotenone treated brains showed extensive neuronal damage whereas with melatonin less damage was observed. Rotenone induces apoptosis via reactive oxygen species production and apoptotic cell death has been identified in PD brains. Furthermore, the apoptotic cell death was detected and quantified by the TUNEL staining. Rotenone treated sections showed signs of apoptosis whereas with melatonin, less apoptotic damage was observed. The findings of this study indicate that the neurohormone, melatonin may protect against rotenone-induced neurodegeneration. Since melatonin production falls substantially during ageing, the loss of this antioxidant is theorized to be instrumental in the degenerative processes associated with advanced age. Considering how devastating diseases such as Parkinson’s disease, are to a patient and the patient’s families, the discovery of protective agents are a matter of urgency. Further investigations using the pesticide model will help to determine the involvement of environmental exposure in the pathogenesis of human diseases as well as to test therapeutic strategies for the treatment of such diseases.
- Full Text:
- Date Issued: 2004
Apartheid South Africa's foreign relations with African states, 1961-1994
- Authors: Pfister, Roger
- Date: 2004
- Subjects: Apartheid -- South Africa -- History -- 20th century Government, Resistance to -- South Africa Democracy -- South Africa South Africa -- Politics and government -- 20th century South Africa -- History -- 1961-1994 South Africa -- Foreign relations -- Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2863 , http://hdl.handle.net/10962/d1007632
- Description: This thesis examines South Africa's foreign relations, viewed from a South African perspective, with the black African countries beyond southern Africa from 1961 to 1994. These relations were determined by the conflict between Pretoria's apartheid ideology on the one hand, and African continental rejection of South Africa's race discrimination policies and its exclusion from the community of African states on the other. The documentary material used primarily stems from the Department of Foreign Affairs archive in Pretoria, supplemented by research conducted in other archives. Furthermore, we conducted interviews and correspondence, and consulted the relevant primary and secondary literature. Given the main source of information, we chose to make this work a case study in Diplomatic History. In consequence, and constituting the core of the study, Chapters 3 to 6 explore the interaction between South Africa and the black African states in a chronological order. At the same time, we draw on the analytical concepts from the academic disciplines of Political Science and its derivative, International Relations, to comprehend developments more fully. We discuss the significance of the approaches from these two disciplines in both the Introduction and Chapter 2. In particular, we emphasise that this study is about Pretoria's foreign policy, involving state and non-state actors, and we suggest that the unequal status between South Africa and the other African states constitutes an inherent factor in the relationship between them. The Conclusion examines the role of the state and non-state actors in determining Pretoria's foreign relations and the relevance of the structural imbalance between South Africa and the black African states in this context.
- Full Text:
- Date Issued: 2004
- Authors: Pfister, Roger
- Date: 2004
- Subjects: Apartheid -- South Africa -- History -- 20th century Government, Resistance to -- South Africa Democracy -- South Africa South Africa -- Politics and government -- 20th century South Africa -- History -- 1961-1994 South Africa -- Foreign relations -- Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2863 , http://hdl.handle.net/10962/d1007632
- Description: This thesis examines South Africa's foreign relations, viewed from a South African perspective, with the black African countries beyond southern Africa from 1961 to 1994. These relations were determined by the conflict between Pretoria's apartheid ideology on the one hand, and African continental rejection of South Africa's race discrimination policies and its exclusion from the community of African states on the other. The documentary material used primarily stems from the Department of Foreign Affairs archive in Pretoria, supplemented by research conducted in other archives. Furthermore, we conducted interviews and correspondence, and consulted the relevant primary and secondary literature. Given the main source of information, we chose to make this work a case study in Diplomatic History. In consequence, and constituting the core of the study, Chapters 3 to 6 explore the interaction between South Africa and the black African states in a chronological order. At the same time, we draw on the analytical concepts from the academic disciplines of Political Science and its derivative, International Relations, to comprehend developments more fully. We discuss the significance of the approaches from these two disciplines in both the Introduction and Chapter 2. In particular, we emphasise that this study is about Pretoria's foreign policy, involving state and non-state actors, and we suggest that the unequal status between South Africa and the other African states constitutes an inherent factor in the relationship between them. The Conclusion examines the role of the state and non-state actors in determining Pretoria's foreign relations and the relevance of the structural imbalance between South Africa and the black African states in this context.
- Full Text:
- Date Issued: 2004
Attitudes of isiXhosa-speaking students at the University of Fort Hare towards the use of isiXhosa as a language of learning and teaching (LOLT)
- Authors: Dalvit, Lorenzo
- Date: 2004
- Subjects: Native language and education -- South Africa -- Eastern Cape , Xhosa language -- Study and teaching (Higher) -- South Africa , English language -- Study and teaching (Higher) -- South Africa , Education, Bilingual , Language and education -- South Africa , Language policy -- South Africa , University of Fort Hare -- Students -- Attitudes
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2343 , http://hdl.handle.net/10962/d1002625 , Native language and education -- South Africa -- Eastern Cape , Xhosa language -- Study and teaching (Higher) -- South Africa , English language -- Study and teaching (Higher) -- South Africa , Education, Bilingual , Language and education -- South Africa , Language policy -- South Africa , University of Fort Hare -- Students -- Attitudes
- Description: The present work presents and discusses the results of a survey of a sample of isiXhosa-speaking students at the University of Fort Hare (Alice campus) and their attitudes towards the possible introduction of isiXhosa as a medium of instruction at this institution. More specifically, the research focuses on, among other things, the students’ attitudes towards English and isiXhosa and their opinions and beliefs about the introduction of dual-mediumship and its possible consequences. The survey methods used are a questionnaire survey as well as follow-up interviews, supplemented by on-campus observation. The results are first analysed as a whole, and then split into different categories according to a set of background variables (gender, year of study, subject studied etc). This analysis indicates that, while English is recognised as the dominant language in South Africa and, more specifically, in the domain of education, some categories of respondents acknowledge the usefulness of isiXhosa as an additional medium of instruction. This survey clearly shows that it makes little sense to present isiXhosa-speaking students with a rigid choice between the existing English-medium and a dual-medium (English and isiXhosa) policy. If dual-mediumship is ever to be implemented, respondents seem to consider the use of isiXhosa as a medium of instruction more appropriate in the first years of study, for selected subjects and in some domains within the academic context rather than others. This study is part of a growing set of surveys on the attitudes of university students towards the use of African languages in education, and can be fruitfully compared with similar research at other institutions. Moreover, the results of the present research can be used to inform future decisions regarding language policy at the University of Fort Hare.
- Full Text:
- Date Issued: 2004
- Authors: Dalvit, Lorenzo
- Date: 2004
- Subjects: Native language and education -- South Africa -- Eastern Cape , Xhosa language -- Study and teaching (Higher) -- South Africa , English language -- Study and teaching (Higher) -- South Africa , Education, Bilingual , Language and education -- South Africa , Language policy -- South Africa , University of Fort Hare -- Students -- Attitudes
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2343 , http://hdl.handle.net/10962/d1002625 , Native language and education -- South Africa -- Eastern Cape , Xhosa language -- Study and teaching (Higher) -- South Africa , English language -- Study and teaching (Higher) -- South Africa , Education, Bilingual , Language and education -- South Africa , Language policy -- South Africa , University of Fort Hare -- Students -- Attitudes
- Description: The present work presents and discusses the results of a survey of a sample of isiXhosa-speaking students at the University of Fort Hare (Alice campus) and their attitudes towards the possible introduction of isiXhosa as a medium of instruction at this institution. More specifically, the research focuses on, among other things, the students’ attitudes towards English and isiXhosa and their opinions and beliefs about the introduction of dual-mediumship and its possible consequences. The survey methods used are a questionnaire survey as well as follow-up interviews, supplemented by on-campus observation. The results are first analysed as a whole, and then split into different categories according to a set of background variables (gender, year of study, subject studied etc). This analysis indicates that, while English is recognised as the dominant language in South Africa and, more specifically, in the domain of education, some categories of respondents acknowledge the usefulness of isiXhosa as an additional medium of instruction. This survey clearly shows that it makes little sense to present isiXhosa-speaking students with a rigid choice between the existing English-medium and a dual-medium (English and isiXhosa) policy. If dual-mediumship is ever to be implemented, respondents seem to consider the use of isiXhosa as a medium of instruction more appropriate in the first years of study, for selected subjects and in some domains within the academic context rather than others. This study is part of a growing set of surveys on the attitudes of university students towards the use of African languages in education, and can be fruitfully compared with similar research at other institutions. Moreover, the results of the present research can be used to inform future decisions regarding language policy at the University of Fort Hare.
- Full Text:
- Date Issued: 2004
Becoming a journalist : a study into the professional socialisation and training of entry-level journalists at the Cape Argus newspaper
- Authors: Maughan, Karyn
- Date: 2004
- Subjects: Argus (Cape Town, South Africa) , Journalism -- Study and teaching -- South Africa , Journalists -- Training of -- South Africa , Journalists -- Selection and appointment -- South Africa , Journalists -- Education -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3507 , http://hdl.handle.net/10962/d1007553 , Argus (Cape Town, South Africa) , Journalism -- Study and teaching -- South Africa , Journalists -- Training of -- South Africa , Journalists -- Selection and appointment -- South Africa , Journalists -- Education -- South Africa
- Description: This thesis attempts to examine the construction of 'professionalism' within the newsroom of the Cape Argus, an English-medium newspaper in post-apartheid South Africa. It is a qualitative study which tries to evaluate how a particular mainstream media discourse of 'professionalism' is enacted and struggled over in the attitudes, behaviour and perceptions of entry-level journalists and news managers at the newspaper. It asks what the process of 'becoming a journalist' requires of entry-level journalists in terms of their previous education and personal qualities - and examines the newsroom strategies employed by news managers when entry-level journalists do not meet these particular requirements. This thesis looks at how the pressures of operating a daily English-language commercial newspaper may shape both the 'professional' expectations of news managers and their ability to positively contribute to entry-level journalists' 'newsroom training'. In attempting to examine the nature of journalistic 'professionalism', this study explores the ideology of knowledge construction within mainstream South African media. Operating from a 'radical democratic' perspective of journalism, which prioritises journalism as a vehicle for diverse social, cultural and political expression, this thesis suggests that South African media education needs to enable journalism students' understanding of the ideological construction of journalistic 'professionalism'.
- Full Text:
- Date Issued: 2004
- Authors: Maughan, Karyn
- Date: 2004
- Subjects: Argus (Cape Town, South Africa) , Journalism -- Study and teaching -- South Africa , Journalists -- Training of -- South Africa , Journalists -- Selection and appointment -- South Africa , Journalists -- Education -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3507 , http://hdl.handle.net/10962/d1007553 , Argus (Cape Town, South Africa) , Journalism -- Study and teaching -- South Africa , Journalists -- Training of -- South Africa , Journalists -- Selection and appointment -- South Africa , Journalists -- Education -- South Africa
- Description: This thesis attempts to examine the construction of 'professionalism' within the newsroom of the Cape Argus, an English-medium newspaper in post-apartheid South Africa. It is a qualitative study which tries to evaluate how a particular mainstream media discourse of 'professionalism' is enacted and struggled over in the attitudes, behaviour and perceptions of entry-level journalists and news managers at the newspaper. It asks what the process of 'becoming a journalist' requires of entry-level journalists in terms of their previous education and personal qualities - and examines the newsroom strategies employed by news managers when entry-level journalists do not meet these particular requirements. This thesis looks at how the pressures of operating a daily English-language commercial newspaper may shape both the 'professional' expectations of news managers and their ability to positively contribute to entry-level journalists' 'newsroom training'. In attempting to examine the nature of journalistic 'professionalism', this study explores the ideology of knowledge construction within mainstream South African media. Operating from a 'radical democratic' perspective of journalism, which prioritises journalism as a vehicle for diverse social, cultural and political expression, this thesis suggests that South African media education needs to enable journalism students' understanding of the ideological construction of journalistic 'professionalism'.
- Full Text:
- Date Issued: 2004
Biochemical characterization of plasmodium falciparum heat shock protein 70
- Matambo, Tonderayi Sylvester
- Authors: Matambo, Tonderayi Sylvester
- Date: 2004
- Subjects: Plasmodium falciparum , Malaria -- Prevention , Protein folding , Proteins -- Purification , Heat shock proteins
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4134 , http://hdl.handle.net/10962/d1015767
- Description: Plamodium falciparum heat shock protein (PfHsp70) is believed to be involved in the cytoprotection of the malaria parasite through its action as a molecular chaperone. Bioinformatic analysis reveal that PfHsp70 consists of the three canonical Hsp70 domains; an ATPase domain of 45 kDa, Substrate binding domain of 15 kDa and a C-terminal domain of 10 kDa. At the C-terminus there is a GGMP repeat motif that is commonly found in Hsp70s of parasitic origins. Plasmodium falciparum genome is 80% A-T rich, making it difficult to recombinantly express its proteins in Escherhia coli (E. coli) as a result of rare codon usage. In this study we carried out experiments to improve expression in E. coli by inserting the PfHsp70 coding region into the pQE30 expression vector. However multiple bands were detected by Western analysis, probably due to the presence of rare codons. The RIG plasmid, which encodes tRNAs for rare codons in particular Arg (AGA/AGG), Ile (AUA) and Gly (GGA) was engineered into the E. coli strain resulting in production of full length PfHsp70. Purification was achieved through Ni²⁺ Chelating sepharose under denaturing conditions. PfHsp70 was found to have a very low basal ATPase activity of 0.262 ± 0.05 nmoles/min/mg of protein. In the presence of reduced and carboxymethylated lactalbumin (RCMLA) a 11-fold increase in ATPase activity was noted whereas in the presence of both RCMLA and Trypanosoma cruzi DnaJ (Tcj2) a 16-fold was achieved. For ATP hydrolysis kcat value of 0.003 min⁻¹ was obtained whereas for ADP release a greater kcat value of 0.8 min⁻¹ was obtained. These results indicated that rate of ATP hydrolysis maybe the rate-determining step in the ATPase cycle of PfHsp70.
- Full Text:
- Date Issued: 2004
- Authors: Matambo, Tonderayi Sylvester
- Date: 2004
- Subjects: Plasmodium falciparum , Malaria -- Prevention , Protein folding , Proteins -- Purification , Heat shock proteins
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4134 , http://hdl.handle.net/10962/d1015767
- Description: Plamodium falciparum heat shock protein (PfHsp70) is believed to be involved in the cytoprotection of the malaria parasite through its action as a molecular chaperone. Bioinformatic analysis reveal that PfHsp70 consists of the three canonical Hsp70 domains; an ATPase domain of 45 kDa, Substrate binding domain of 15 kDa and a C-terminal domain of 10 kDa. At the C-terminus there is a GGMP repeat motif that is commonly found in Hsp70s of parasitic origins. Plasmodium falciparum genome is 80% A-T rich, making it difficult to recombinantly express its proteins in Escherhia coli (E. coli) as a result of rare codon usage. In this study we carried out experiments to improve expression in E. coli by inserting the PfHsp70 coding region into the pQE30 expression vector. However multiple bands were detected by Western analysis, probably due to the presence of rare codons. The RIG plasmid, which encodes tRNAs for rare codons in particular Arg (AGA/AGG), Ile (AUA) and Gly (GGA) was engineered into the E. coli strain resulting in production of full length PfHsp70. Purification was achieved through Ni²⁺ Chelating sepharose under denaturing conditions. PfHsp70 was found to have a very low basal ATPase activity of 0.262 ± 0.05 nmoles/min/mg of protein. In the presence of reduced and carboxymethylated lactalbumin (RCMLA) a 11-fold increase in ATPase activity was noted whereas in the presence of both RCMLA and Trypanosoma cruzi DnaJ (Tcj2) a 16-fold was achieved. For ATP hydrolysis kcat value of 0.003 min⁻¹ was obtained whereas for ADP release a greater kcat value of 0.8 min⁻¹ was obtained. These results indicated that rate of ATP hydrolysis maybe the rate-determining step in the ATPase cycle of PfHsp70.
- Full Text:
- Date Issued: 2004
Capsule immobilisation of sulphate-reducing bacteria and application in disarticulated systems
- Authors: Sanyahumbi, Douglas
- Date: 2004
- Subjects: Sulfur bacteria , Water -- Purification -- Biological treatment
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3935 , http://hdl.handle.net/10962/d1003994
- Description: Biotechnology of sulphate reducing bacteria has developed rapidly in recent years with the recognition of their extensive and diverse biocatalytic potential. However, their application in a number of areas has been constrained due to problems including poor cell retention within the continuous bioprocess reactor environment, and contamination of the treated stream with residual organic feed components and cell biomass. These problems have so far excluded the application of biological sulphate reduction in the treatment of ‘clean’ inorganic waste streams where components such as sulphate, acidity and heavy metal contamination require treatment. This study investigated the effective immobilisation of sulphate reducing bacterial cultures and proposed that the disarticulation of the electron donor and carbon source supply using such systems would create the basis for their application in the treatment of ‘clean’ inorganic waste streams. A functional and stable sulphate reducing culture was selected and following evaluation using a number of techniques, was immobilised by encapsulation within a calcium-alginate-xanthum gum membrane to give robust capsules with good sulphate reduction activity. The concept of disarticulation was investigated in a swing-back cycle where the carbon source was excluded and the electron donor supplied in the form of hydrogen gas in a continuous up-flow capsule-packed column reactor. Following a period of operation in this mode (4-12 days), the system was swung back to a carbon feed to supply requirements of cell maintenance (2-3 days). Three types of synthetic ‘clean’ inorganic waste stream treatments were investigated, including sulphate removal, neutralisation of acidity and heavy metal (copper and lead) removal. The results showed: • Sulphate removal at a rate of 50 mg SO₄²⁻L/day/g initial wet mass of capsules during three 4-day cycles of electron donor phase. This was comparable to the performance of free cell systems; • Neutralisation of acidity where influent pH values of 2.4 and 4.0 were elevated to above pH 7.5; • Copper removal of 99 and 85 % was achieved with initial copper concentrations of 2 and 60 mg/L respectively; • Percentage lead removal values of 49 and 78 % were achieved; This first report on the application of the concept of capsular immobilisation and disarticulation in the treatment of ‘clean’ inorganic waste streams will require future studies in order to extend the development of the full potential of the concept.
- Full Text:
- Date Issued: 2004
- Authors: Sanyahumbi, Douglas
- Date: 2004
- Subjects: Sulfur bacteria , Water -- Purification -- Biological treatment
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3935 , http://hdl.handle.net/10962/d1003994
- Description: Biotechnology of sulphate reducing bacteria has developed rapidly in recent years with the recognition of their extensive and diverse biocatalytic potential. However, their application in a number of areas has been constrained due to problems including poor cell retention within the continuous bioprocess reactor environment, and contamination of the treated stream with residual organic feed components and cell biomass. These problems have so far excluded the application of biological sulphate reduction in the treatment of ‘clean’ inorganic waste streams where components such as sulphate, acidity and heavy metal contamination require treatment. This study investigated the effective immobilisation of sulphate reducing bacterial cultures and proposed that the disarticulation of the electron donor and carbon source supply using such systems would create the basis for their application in the treatment of ‘clean’ inorganic waste streams. A functional and stable sulphate reducing culture was selected and following evaluation using a number of techniques, was immobilised by encapsulation within a calcium-alginate-xanthum gum membrane to give robust capsules with good sulphate reduction activity. The concept of disarticulation was investigated in a swing-back cycle where the carbon source was excluded and the electron donor supplied in the form of hydrogen gas in a continuous up-flow capsule-packed column reactor. Following a period of operation in this mode (4-12 days), the system was swung back to a carbon feed to supply requirements of cell maintenance (2-3 days). Three types of synthetic ‘clean’ inorganic waste stream treatments were investigated, including sulphate removal, neutralisation of acidity and heavy metal (copper and lead) removal. The results showed: • Sulphate removal at a rate of 50 mg SO₄²⁻L/day/g initial wet mass of capsules during three 4-day cycles of electron donor phase. This was comparable to the performance of free cell systems; • Neutralisation of acidity where influent pH values of 2.4 and 4.0 were elevated to above pH 7.5; • Copper removal of 99 and 85 % was achieved with initial copper concentrations of 2 and 60 mg/L respectively; • Percentage lead removal values of 49 and 78 % were achieved; This first report on the application of the concept of capsular immobilisation and disarticulation in the treatment of ‘clean’ inorganic waste streams will require future studies in order to extend the development of the full potential of the concept.
- Full Text:
- Date Issued: 2004
Changes in energy use patterns in the Bushbuckridge Lowveld of the Limpopo Province, South Africa: eleven years on
- Authors: Madubansi, Mainza
- Date: 2004
- Subjects: Energy consumption -- South Africa -- Limpopo
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4756 , http://hdl.handle.net/10962/d1007139 , Energy consumption -- South Africa -- Limpopo
- Description: This research reports on the energy transition that has taken place in the Bushbuckridge district between 1991 and 2002. It is a follow up to a similar study that was done in 1991 investigating a number of aspects of energy use. It uses the 1991 study as a baseline and aims to explore how the passage of time, growth of the local population and changes in incomes and the availability of fuels have affected the patterns of fuel use in the region in the past eleven years. A structured interview approach was used for most aspects of the study. The interview schedule included sections on types, amounts and reasons for use and non-use of 13 different energy sources as well as data on income levels, household size and other economic parameters. Analysis of the consumption patterns of the different fuel types revealed that between the two survey periods, households in the sample settlements went through some pronounced changes in patterns of fuel use, particularly those that had acquired electricity. The introduction of electricity in the region had certainly played a major role in spurring the energy transition. Fuels that were previously used for lighting, powering entertainment appliances and refrigeration had been displaced by electricity. In terms of cooking and other thermal application, however, the vast majority of households in all the sample settlements continued using fuelwood and complemented it to a lesser extent with paraffin and electricity. In both surveys, the use or non-use of available fuels in the region was influenced by several factors. Common reasons for non-use of certain fuel types included expense, lack of appliances, the risk involved in using such fuels and the preference for other fuels. Reasons for use were mainly related to the low cost of the fuel and the fuel’s ability to meet particular end uses. Although incomes in the region had increased between 1991 and 2002, they were still below the poverty line. Activities from which households obtained their income remained the same. Old age pension, migrant wages and the informal sector remained the largest contributors of income to most households. Other indicators of relative wealth and poverty, like ownership of vehicles, bicycles and beds remained the same.
- Full Text:
- Date Issued: 2004
- Authors: Madubansi, Mainza
- Date: 2004
- Subjects: Energy consumption -- South Africa -- Limpopo
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4756 , http://hdl.handle.net/10962/d1007139 , Energy consumption -- South Africa -- Limpopo
- Description: This research reports on the energy transition that has taken place in the Bushbuckridge district between 1991 and 2002. It is a follow up to a similar study that was done in 1991 investigating a number of aspects of energy use. It uses the 1991 study as a baseline and aims to explore how the passage of time, growth of the local population and changes in incomes and the availability of fuels have affected the patterns of fuel use in the region in the past eleven years. A structured interview approach was used for most aspects of the study. The interview schedule included sections on types, amounts and reasons for use and non-use of 13 different energy sources as well as data on income levels, household size and other economic parameters. Analysis of the consumption patterns of the different fuel types revealed that between the two survey periods, households in the sample settlements went through some pronounced changes in patterns of fuel use, particularly those that had acquired electricity. The introduction of electricity in the region had certainly played a major role in spurring the energy transition. Fuels that were previously used for lighting, powering entertainment appliances and refrigeration had been displaced by electricity. In terms of cooking and other thermal application, however, the vast majority of households in all the sample settlements continued using fuelwood and complemented it to a lesser extent with paraffin and electricity. In both surveys, the use or non-use of available fuels in the region was influenced by several factors. Common reasons for non-use of certain fuel types included expense, lack of appliances, the risk involved in using such fuels and the preference for other fuels. Reasons for use were mainly related to the low cost of the fuel and the fuel’s ability to meet particular end uses. Although incomes in the region had increased between 1991 and 2002, they were still below the poverty line. Activities from which households obtained their income remained the same. Old age pension, migrant wages and the informal sector remained the largest contributors of income to most households. Other indicators of relative wealth and poverty, like ownership of vehicles, bicycles and beds remained the same.
- Full Text:
- Date Issued: 2004
Characterisation of the J domain aminoacid residues important for the interaction of DNAJ-like proteins with HSP70 chaperones
- Authors: Hennessy, Fritha
- Date: 2004
- Subjects: Heat shock proteins Protein folding Proteins -- Analysis Proteins -- Structure Molecular chaperones
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3937 , http://hdl.handle.net/10962/d1003996
- Description: The 70 kDa heat shock proteins (Hsp70s) are vital for normal protein folding, as they stabilise the unfolded state of nascent polypeptides, allowing these sufficient time to attain a correct tertiary structure. Hsp70s are aided by the DnaJ-like family of proteins, which interact with Hsp70s in order to enhance the chaperone activity of these proteins. DnaJ-like proteins contain a J domain, a seventy amino acid domain consisting of four α-helices, which is defined by the presence of an invariant tripeptide of histidine, proline and aspartic acid (HPD motif). This motif is key to the interaction between DnaJ-like proteins and Hsp70s. This thesis has focused on determining the presence of other conserved residues in the J domain and their role in mediating the interaction of DnaJ-like proteins with partner Hsp70s. DnaJ-like proteins from Agrobacterium tumefaciens RUOR were isolated and used as a model system. A. tumefaciens DnaJ (Agt DnaJ) was able to replace the lack of E. coli DnaJ in an E. coli null mutant strain, however, additional A. tumefaciens DnaJ-like proteins Agt DjC1/DjlA, Agt DjC2 and Agt DjC5 were unable to complement for the lack of E. coli DnaJ. Replacement of the Agt DnaJ J domain with J domains from these proteins resulted in non-functional chimeric proteins, despite some sequence conservation. The kinetics of the basal specific ATPase activity of Agt DnaK, and its ability to have this activity stimulated by Agt DnaJ and Agt DnaJ-H33Q were also investigated. Stimulation of the ATPase activity by Agt DnaJ ranged between 1.5 to 2 fold, but Agt DnaJ-H33Q was unable to stimulate the basal ATPase activity. Conserved amino acids in the J domain were identified in silico, and these residues were substituted in the J domain of Agt DnaJ. The ability of these derivative proteins to replace E. coli DnaJ was investigated. Alterations in the HPD motif gave rise to proteins unable to complement for lack of E. coli DnaJ, consistent with literature. Agt DnaJ-R26A was unable to replace E. coli DnaJ suggesting that Arg26 could be key to the interaction with partner Hsp70s. Agt DnaJ-D59A was unable to replace E. coli DnaJ; substitutions in Asp59 have not previously been shown to impact on the function of DnaJ. Substituting Arg63 in Agt DnaJ abrogated the levels of complementation. Substitution of several structural residues was also found to disrupt the in vivo function of Agt DnaJ suggesting that the maintenance of the structural integrity of the J domain was important for function. This study has identified a number of residues critical to the structure and function of the J domain of Agt DnaJ, and potentially of general importance as molecular determinants for DnaJ-Hsp70 interaction.
- Full Text:
- Date Issued: 2004
- Authors: Hennessy, Fritha
- Date: 2004
- Subjects: Heat shock proteins Protein folding Proteins -- Analysis Proteins -- Structure Molecular chaperones
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3937 , http://hdl.handle.net/10962/d1003996
- Description: The 70 kDa heat shock proteins (Hsp70s) are vital for normal protein folding, as they stabilise the unfolded state of nascent polypeptides, allowing these sufficient time to attain a correct tertiary structure. Hsp70s are aided by the DnaJ-like family of proteins, which interact with Hsp70s in order to enhance the chaperone activity of these proteins. DnaJ-like proteins contain a J domain, a seventy amino acid domain consisting of four α-helices, which is defined by the presence of an invariant tripeptide of histidine, proline and aspartic acid (HPD motif). This motif is key to the interaction between DnaJ-like proteins and Hsp70s. This thesis has focused on determining the presence of other conserved residues in the J domain and their role in mediating the interaction of DnaJ-like proteins with partner Hsp70s. DnaJ-like proteins from Agrobacterium tumefaciens RUOR were isolated and used as a model system. A. tumefaciens DnaJ (Agt DnaJ) was able to replace the lack of E. coli DnaJ in an E. coli null mutant strain, however, additional A. tumefaciens DnaJ-like proteins Agt DjC1/DjlA, Agt DjC2 and Agt DjC5 were unable to complement for the lack of E. coli DnaJ. Replacement of the Agt DnaJ J domain with J domains from these proteins resulted in non-functional chimeric proteins, despite some sequence conservation. The kinetics of the basal specific ATPase activity of Agt DnaK, and its ability to have this activity stimulated by Agt DnaJ and Agt DnaJ-H33Q were also investigated. Stimulation of the ATPase activity by Agt DnaJ ranged between 1.5 to 2 fold, but Agt DnaJ-H33Q was unable to stimulate the basal ATPase activity. Conserved amino acids in the J domain were identified in silico, and these residues were substituted in the J domain of Agt DnaJ. The ability of these derivative proteins to replace E. coli DnaJ was investigated. Alterations in the HPD motif gave rise to proteins unable to complement for lack of E. coli DnaJ, consistent with literature. Agt DnaJ-R26A was unable to replace E. coli DnaJ suggesting that Arg26 could be key to the interaction with partner Hsp70s. Agt DnaJ-D59A was unable to replace E. coli DnaJ; substitutions in Asp59 have not previously been shown to impact on the function of DnaJ. Substituting Arg63 in Agt DnaJ abrogated the levels of complementation. Substitution of several structural residues was also found to disrupt the in vivo function of Agt DnaJ suggesting that the maintenance of the structural integrity of the J domain was important for function. This study has identified a number of residues critical to the structure and function of the J domain of Agt DnaJ, and potentially of general importance as molecular determinants for DnaJ-Hsp70 interaction.
- Full Text:
- Date Issued: 2004
Cleaning of fouled membranes using enzymes from a sulphidogenic bioreactor
- Authors: Melamane, Xolisa
- Date: 2004
- Subjects: Membrane filters , Membrane filters -- Fouling , Enzymes -- Biotechnology , Enzymes -- Purification , Water -- Purification -- Membrane filtration , Ultrafiltration
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4133 , http://hdl.handle.net/10962/d1015764
- Description: Maintenance of membrane performance requires inevitable cleaning or defouling of fouled membranes. Membrane cleaning using enzymes such as proteases, lipases, α-glucosidases from a sulphidogenic bioreactor was investigated. At first, dilute and concentrated enzyme extract were prepared form the sulphidogenic pellet. Enzyme assays on 0.5 % azocaisen, 1 % triacetin and 1 mg/ml ρ-nitrophenyl-α-D-glucopyranoside were performed using the concentrated enzyme extract (0 – 200 mg/ml). For membrane fouling, an abattoir effluent was obtained from Ostritech Pty (Ltd), Grahamstown, South Africa. The effluent was characterised for presence of potential foulants such as lipids, proteins, amino acids and carbohydrates. Static fouling of polysulphone membranes (0.22 μm, 47 mm) was then performed using the abattoir effluent. Cleaning of the fouled membranes was also performed using at first the dilute and then the concentrated form (200 mg/ml) of enzyme extracts. Qualitative and quantitative biochemical analysis for proteins, lipids and carbohydrates was performed to ascertain the presence of foulants on polysulphone membranes and their removal by dilute or concentrated enzyme extracts. The ability of dilute enzyme extracts to remove proteins lipids, and carbohydrates fouling capillary UF membrane module; their ability to restore permeate fluxes and transmembrane pressure after cleaning/defouling was also investigated. Permeate volumes from this UF membrane module were analysed for protein, amino acids, lipids, and carbohydrates concentrations after fouling and defouling. Fouling was further characterized by standard blocking, cake filtration and pore blocking models using stirred UF cell and polyethersulphone membranes with MWCO of 30 000, 100 000 and 300 000. After characterization of fouling, polyethersulphone membranes with MWCO of 30 000 and 300 000 were defouled using the concentrated enzyme extract (100 mg ml). Enzyme activities at 200 mg/ml of enzyme concentration were 8.071 IU, 86.71 IU and 789.02 IU for proteases, lipases and α-glucosidases. The abattoir effluent contained 553 μg/ml of lipid, 301 μg/ml of protein, 141 μg/ml of total carbohydrate, and 0.63 μg/ml of total reducing sugars. Proteins, lipids and carbohydrates fouling polysulphone membranes after a day were removed by 23.4 %, when a dilute enzyme was used. A concentrated enzyme extract of 200 mg/ml was able to remove proteins, lipids and carbohydrates up to 5 days of fouling by 100 %, 82 %, 71 %, 68 % and 76 % respectively. Defouling of dynamically fouled capillary ultrafiltration membranes using sulphidogenic proteases was successful at pH 10, 37°C, within 1 hour. Sulphidogenic proteases activity was 2.1 U/ml and flux Recovery (FR %) was 64. Characterization of fouling revealed that proteins and lipids were major foulants while low concentration of carbohydrates fouled polyethersulphone membranes. Fouling followed standard blocking for 10 minutes in all the membranes; afterwards fouling adopted cake filtration model for membranes with 30 000 MWCO and pore blocking model for membranes with 300 000 MWCO. A concentration of 100 mg/ml of enzyme extract was able to remove fouling from membranes with MWCO of 30 000. Defouling membranes that followed pore blocking model i.e. 300 000 MWCO was not successful due to a mass transfer problem. From the results of defouling of 30 000 and 300 000 MWCO it was concluded that defouling of cake layer fouling (30 000 MWCO) was successful while defouling of pore blocking fouling was unsuccessful due to a mass transfer problem. The ratio of enzymes present in the enzyme extract when calculated based on enzymatic activity for proteases, lipases and α-glucosidases was 1.1 %, 11 % and 87.9 %. It was hypothesized that apart from proteases, lipases, α and β-glucosidases; phosphatases, sulphatases, amonipeptidases etc. from a sulphidogenic bioreactor clean or defoul cake layer fouling by organic foulants and pore blocking fouling provided the mass transfer problem is solved. However, concentration of enzymes from a sulphidogenic bioreactor has not been optimized yet. Other methods of concentrating the enzyme extract can be investigated for example use of organic solvents.
- Full Text:
- Date Issued: 2004
- Authors: Melamane, Xolisa
- Date: 2004
- Subjects: Membrane filters , Membrane filters -- Fouling , Enzymes -- Biotechnology , Enzymes -- Purification , Water -- Purification -- Membrane filtration , Ultrafiltration
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4133 , http://hdl.handle.net/10962/d1015764
- Description: Maintenance of membrane performance requires inevitable cleaning or defouling of fouled membranes. Membrane cleaning using enzymes such as proteases, lipases, α-glucosidases from a sulphidogenic bioreactor was investigated. At first, dilute and concentrated enzyme extract were prepared form the sulphidogenic pellet. Enzyme assays on 0.5 % azocaisen, 1 % triacetin and 1 mg/ml ρ-nitrophenyl-α-D-glucopyranoside were performed using the concentrated enzyme extract (0 – 200 mg/ml). For membrane fouling, an abattoir effluent was obtained from Ostritech Pty (Ltd), Grahamstown, South Africa. The effluent was characterised for presence of potential foulants such as lipids, proteins, amino acids and carbohydrates. Static fouling of polysulphone membranes (0.22 μm, 47 mm) was then performed using the abattoir effluent. Cleaning of the fouled membranes was also performed using at first the dilute and then the concentrated form (200 mg/ml) of enzyme extracts. Qualitative and quantitative biochemical analysis for proteins, lipids and carbohydrates was performed to ascertain the presence of foulants on polysulphone membranes and their removal by dilute or concentrated enzyme extracts. The ability of dilute enzyme extracts to remove proteins lipids, and carbohydrates fouling capillary UF membrane module; their ability to restore permeate fluxes and transmembrane pressure after cleaning/defouling was also investigated. Permeate volumes from this UF membrane module were analysed for protein, amino acids, lipids, and carbohydrates concentrations after fouling and defouling. Fouling was further characterized by standard blocking, cake filtration and pore blocking models using stirred UF cell and polyethersulphone membranes with MWCO of 30 000, 100 000 and 300 000. After characterization of fouling, polyethersulphone membranes with MWCO of 30 000 and 300 000 were defouled using the concentrated enzyme extract (100 mg ml). Enzyme activities at 200 mg/ml of enzyme concentration were 8.071 IU, 86.71 IU and 789.02 IU for proteases, lipases and α-glucosidases. The abattoir effluent contained 553 μg/ml of lipid, 301 μg/ml of protein, 141 μg/ml of total carbohydrate, and 0.63 μg/ml of total reducing sugars. Proteins, lipids and carbohydrates fouling polysulphone membranes after a day were removed by 23.4 %, when a dilute enzyme was used. A concentrated enzyme extract of 200 mg/ml was able to remove proteins, lipids and carbohydrates up to 5 days of fouling by 100 %, 82 %, 71 %, 68 % and 76 % respectively. Defouling of dynamically fouled capillary ultrafiltration membranes using sulphidogenic proteases was successful at pH 10, 37°C, within 1 hour. Sulphidogenic proteases activity was 2.1 U/ml and flux Recovery (FR %) was 64. Characterization of fouling revealed that proteins and lipids were major foulants while low concentration of carbohydrates fouled polyethersulphone membranes. Fouling followed standard blocking for 10 minutes in all the membranes; afterwards fouling adopted cake filtration model for membranes with 30 000 MWCO and pore blocking model for membranes with 300 000 MWCO. A concentration of 100 mg/ml of enzyme extract was able to remove fouling from membranes with MWCO of 30 000. Defouling membranes that followed pore blocking model i.e. 300 000 MWCO was not successful due to a mass transfer problem. From the results of defouling of 30 000 and 300 000 MWCO it was concluded that defouling of cake layer fouling (30 000 MWCO) was successful while defouling of pore blocking fouling was unsuccessful due to a mass transfer problem. The ratio of enzymes present in the enzyme extract when calculated based on enzymatic activity for proteases, lipases and α-glucosidases was 1.1 %, 11 % and 87.9 %. It was hypothesized that apart from proteases, lipases, α and β-glucosidases; phosphatases, sulphatases, amonipeptidases etc. from a sulphidogenic bioreactor clean or defoul cake layer fouling by organic foulants and pore blocking fouling provided the mass transfer problem is solved. However, concentration of enzymes from a sulphidogenic bioreactor has not been optimized yet. Other methods of concentrating the enzyme extract can be investigated for example use of organic solvents.
- Full Text:
- Date Issued: 2004
Composition portfolio
- Authors: Prince, Lloyd T
- Date: 2004
- Subjects: Music -- South Africa Composition (Music)
- Language: English
- Type: Thesis , Masters , MMus
- Identifier: vital:2652 , http://hdl.handle.net/10962/d1002318
- Full Text:
- Date Issued: 2004
- Authors: Prince, Lloyd T
- Date: 2004
- Subjects: Music -- South Africa Composition (Music)
- Language: English
- Type: Thesis , Masters , MMus
- Identifier: vital:2652 , http://hdl.handle.net/10962/d1002318
- Full Text:
- Date Issued: 2004
Contested rights : the impact of game farming on farm workers in the Bushmen's River area
- Authors: Luck, Kelly
- Date: 2004
- Subjects: Game farms -- South Africa -- Eastern Cape , Agricultural laborers -- South Africa -- Eastern Cape -- Social conditions Agricultural laborers -- South Africa -- Eastern Cape -- Economic conditions Agricultural laborers -- Political aspects -- South Africa -- Eastern Cape Agricultural laborers -- Legal status, laws, etc. -- South Africa -- Eastern Cape Agricultural laborers -- Protection -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2104 , http://hdl.handle.net/10962/d1004144
- Description: This thesis is an investigation of the impact of commercial game farming on former farm workers in the Bushman's River area of the Eastern Cape. In its examination of the broader economic and political changes that have facilitated a move from agriculture to game farming, it analyses how these changes affect farm workers. The main concern of the thesis is the ways in which farm workers (at the local level) respond to changes at the national and global level (legal and political changes, the advent of tourism, and the injection of foreign capital and businessmen into the area). Lack of knowledge about their rights under the current political dispensation, as well as the perceived need for mediation between themselves and foreign landowners, points to a general sense of powerlessness. Feelings of alienation from local government structures aimed at fulfilling this function indicate a significant gap between the statute at the national level and the local reality. Local reality is informed by a strong conservatism which is generated by African Independent Church structures and local Xhosa perceptions of manhood and respectability. This conservative discourse leads to a frame of reference which is largely informed by pre-1994 interactions with farmers and government. This results in a situation in which farm workers, largely unaware of their rights in the new dispensation, operate as they did in the past; waiting for landowners to decide their fate for them. What ensues is a lack of meaningful interaction with government and landowners, perpetuating their subjugation and cynicism as to whether government structures are in fact working in their interests. The thesis comes to three main conclusions. The first is that game farming has been negatively received by farm workers due to the associated threats of unemployment and eviction. The second is that despite high levels of subjugation, even the very poor are agents to some degree. The creation of a masculine identity which is internally articulated, as opposed to outwardly expressed, and the grounding of reputation in the family suggest that farm workers have developed mechanisms to deal with their disempowered position. Lastly, farm workers are in possession of social capital which has made it possible for them to deal with their low status in the societal hierarchy. This includes the Church, family and fellow community members. These coping strategies have however proved a disadvantage in the current era because they prevent direct communication with landowners, government and NGOs.
- Full Text:
- Date Issued: 2004
- Authors: Luck, Kelly
- Date: 2004
- Subjects: Game farms -- South Africa -- Eastern Cape , Agricultural laborers -- South Africa -- Eastern Cape -- Social conditions Agricultural laborers -- South Africa -- Eastern Cape -- Economic conditions Agricultural laborers -- Political aspects -- South Africa -- Eastern Cape Agricultural laborers -- Legal status, laws, etc. -- South Africa -- Eastern Cape Agricultural laborers -- Protection -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2104 , http://hdl.handle.net/10962/d1004144
- Description: This thesis is an investigation of the impact of commercial game farming on former farm workers in the Bushman's River area of the Eastern Cape. In its examination of the broader economic and political changes that have facilitated a move from agriculture to game farming, it analyses how these changes affect farm workers. The main concern of the thesis is the ways in which farm workers (at the local level) respond to changes at the national and global level (legal and political changes, the advent of tourism, and the injection of foreign capital and businessmen into the area). Lack of knowledge about their rights under the current political dispensation, as well as the perceived need for mediation between themselves and foreign landowners, points to a general sense of powerlessness. Feelings of alienation from local government structures aimed at fulfilling this function indicate a significant gap between the statute at the national level and the local reality. Local reality is informed by a strong conservatism which is generated by African Independent Church structures and local Xhosa perceptions of manhood and respectability. This conservative discourse leads to a frame of reference which is largely informed by pre-1994 interactions with farmers and government. This results in a situation in which farm workers, largely unaware of their rights in the new dispensation, operate as they did in the past; waiting for landowners to decide their fate for them. What ensues is a lack of meaningful interaction with government and landowners, perpetuating their subjugation and cynicism as to whether government structures are in fact working in their interests. The thesis comes to three main conclusions. The first is that game farming has been negatively received by farm workers due to the associated threats of unemployment and eviction. The second is that despite high levels of subjugation, even the very poor are agents to some degree. The creation of a masculine identity which is internally articulated, as opposed to outwardly expressed, and the grounding of reputation in the family suggest that farm workers have developed mechanisms to deal with their disempowered position. Lastly, farm workers are in possession of social capital which has made it possible for them to deal with their low status in the societal hierarchy. This includes the Church, family and fellow community members. These coping strategies have however proved a disadvantage in the current era because they prevent direct communication with landowners, government and NGOs.
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- Date Issued: 2004