A chemical investigation of Tulbaghia Violacea
- Authors: Burton, Stephanie Gail
- Date: 1990
- Subjects: Liliaceae , Plants -- Analysis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4528 , http://hdl.handle.net/10962/d1015725
- Description: Tulbaghia violacea, a member of the family Alliaceae is indigenous to the Eastern Cape and is widely used as a herbal remedy for various febrile and gastro-enteric ailments, particularly in young children. Adverse effects, and even fatalities, have been reported following treatment with the plant extract. The project has involved synthesis of model compounds, chromatographic analysis of flavonoid and other constituents of the plant, and examination of the volatile components. Some fifteen flavones were synthesised as chromatographic models and in the course of this work, the development of a new method for synthesis of carboxylic anhydrides was completed. Use of the flavone standards permitted identification of the flavonols kaempferol and quercetin in hydrolysed glycosidic plant extracts. In addition, several sugars were identified, viz., D-glucose, D-fructose, L-arabinose and D-galactose as free sugars, and D-glucose, D-galactose , 1-rhamnose, D- fucose, D-xylose, 1-arabinose and D-fructose as glycosidic sugars, by g.l.c. and g. c. - m. s. analysis of derivatives of isolated sugar mixtures. The presence in the plant extracts of steroidal saponins was also demonstrated. The sulphur compounds, 2,4,5,7-tetrathiaoctane-2,2-dioxide and 2,4,5,7-tetrathiaoctane were isolated from the plant and characterised spectroscopically. This result, together with analysis of volatiles from the plant, has led to a proposal concerning the nature and origin of sulphur compounds in Tulbaghia violacea, showing close correlation with the sulphur compounds in Allium species. Investigation of the biological activity of Tulbaghia violacea extracts showed bacteriostatic activity, particularly of extracts which had not been heated, and which had been prepared from mature plants. Treatment of isolated smooth muscle preparations with Tulbaghia violacea extracts indicated the presence of a β-adrenergic agonist having an inhibitory effect on normal muscle contraction. The results of the investigations indicate that while there may be some basis for use of the plant as an antibacterial, or to treat colic, the adverse effects, caused possibly by the sulphur compounds and/or steroidal saponins present, may override the beneficial effects.
- Full Text:
- Date Issued: 1990
A life-history approach to the biology of Oreochromis mossambicus (Pisces : Cichlidae) in the Eastern Cape, South Africa
- Authors: James, Nick, 1955-
- Date: 1990
- Subjects: Cichlids -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5187 , http://hdl.handle.net/10962/d1001963
- Description: The phenotypic plasticity of the cichlid Oreochromis mossambicus was examined in fresh and brackish water populations living in the eastern Cape near the southern extremity of the natural range. The primary goal of the study was to examine the wide variety of life-history styles which were expressed in relation to the varying environmental conditions. Four natural populations were studied during 1986 and 1987, and some experimental work under artificial conditions was carried out at the Rhodes University fishfarm during 1988. This comparative approach gave insights into the complex interactions between biotic and abiotic factors which are exploited by this hardy species. O. mossambicus displayed a wide variety of life-history tactics under harsh environmental conditions. The growth of juvenile fish was rapid and mortality of the young-of-the-year was high. Age at first onset of sexual maturity ranged from one to three years. Under harsh conditions growth rate was markedly reduced after maturity, which occurred during the second or third summer and growth disparity between the sexes was more pronounced. Longevity was found to vary from five to ten years for different populations. Under more favourable conditions (resembling the typical "floodplain" habitats of this species at lower latitudes) very rapid juvenile growth, and a high maximum mass of over 2300 g for both sexes was iii achieved. Sexual maturity under these conditions could be delayed until the third or fourth summer of life. O. mossambicus demonstrated an ability to withstand and even benefit from fluctuating water levels, and adjusted its life style according to the prevailing conditions: at times of high water level recruitment was low and sexual maturity delayed. During low water periods reproductive rates increased. Q. mossambicus showed a distinct preference for gentle gradients and well-vegetated habitats for feeding, nesting and refuge. During the study winter temperatures were near and at some times below the generally accepted lower limits for this species. This implies that these indigenous populations have a degree of cold tolerance. A further implication is that Q. mossambicus has the ability to thrive under adverse conditions which may partially explain its wide and successful colonisation of water bodies far from its natural range, and its obvious fisheries potential.
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- Date Issued: 1990
A preliminary appraisal of the mineral potential of Venda based on a reconnaissance geochemical soil sampling survey and literature review
- Authors: Wilson, Michael George Carey
- Date: 1990
- Subjects: Mines and mineral resources -- South Africa -- Venda
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4994 , http://hdl.handle.net/10962/d1005606 , Mines and mineral resources -- South Africa -- Venda
- Description: A reconnaissance soil sampling survey was carried out over Venda by Cycad (Pty) Ltd and the samples were analysed for 36 elements using XRF techniques, by Anglo American Research Laboratories in Johanuesburg. The data resulting from this survey forms the basis for the present interpretive study. Initially the sample positions were co-ordinated, then the geological, soil and sample types were allocated to each point. Twelve lithological groupings were chosen which incorporated most of the available data, retained significant geological characteristics and consisted of statistically significant sample populations. Statistical manipulation was undertaken for each of the lithological groups. Using a final population of 5768 samples, means were determined and anomalous values were identified using a threshold of mean plus two standard deviations. Due to time and budgetary constraints, 24 of the 36 elements were chosen for statisical manipulation and fourteen of these, with particular economic significance, were chosen for plotting, wherever significant numbers of anomalies were present. The element overlays were plotted so as to coincide with 18 of the 25, 1:50 000 topographic sheets covering Venda , the remaining 7 having inadequate sample coverage to yield meaningful contours. In this way a total of 175 element overlay sheets were plotted, each showing contoured element levels, with selected anomalous values. The treatment of the vast body of information made available by the Cycad sampling programme has thus been selective and has continually been aimed at highlighting and concentrating attention on the areas of greatest indicated mineralization potential, rather than on specific anomalies. In this regard it is felt that the present study has been successful, in spite of limited sample coverage in some areas. Combining the results of this study with a modern tectonically-based appraisal of mineralization potential and a knowledge of the local geology and previously known mineralization gleaned from an extensive literature review, the following types of mineralization are considered to have the highest potential in Venda: i) Nickel-copper-platinum mineralization as well as magnesite, in the olivine dolerite sills which intrude the base of the Karoo Sequence in Northern Venda. ii) Coal in the basal Karoo Sequence sediments in a broad zone from Jazz 715 MS in the west, and along the Klein Tshipise fault from Amonda 159 MT to the Mutale Copper Fields then east of these to the Kruger National Park. Where intrusives invade these lower Karoo sed iments the potential exists for amorphous graphite. iii) Hydrothermal copper and possibly gold and silver concentrations, in Nzhelele and Sibasa Formation rocks, particularly those associated with faults known to have been active in post-Soutpansberg times. iv) Sediment-hosted massive sulphide deposits (Cu-Pb -Zn) close to basin margin faults, near intersections with cross cutting faults that have resulted in localized basin formation. These are most likely in the Soutpansberg sediments. v) Martle, flake-graphite and late stage skarn mineralization (including lead, zinc, gold and tungsten), in calcareous rocks of the Gumbu Formation.
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- Date Issued: 1990
A study of coeval sibling cannibalism in larval and juvenile fishes and its control under culture conditions
- Authors: Pienaar, Anthony Graham
- Date: 1990
- Subjects: Fishes -- Behavior , Fishes -- Larvae , Fish culture
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5781 , http://hdl.handle.net/10962/d1005469 , Fishes -- Behavior , Fishes -- Larvae , Fish culture
- Description: The primary objective of this study was to examine environmental parameters thought to affect cannibalism in certain fish species. It was found that environmental, behavioural, genetic and physiological factors all affect cannibalism in the species exhibiting the phenomenon. The diversity of factors nfluencing cannibalism served to illustrate the complexity of this behaviour pattern. Feeding to satiation was found to suppress cannibalism in catfish, trout and koi carp. High population densities were found to increase the rate of cannibalism, thereby acting as a population regulation mechanism for catfish, trout and the common and koi carps. Live food, as compared with dry pelletized feed was found to significantly suppress cannibalistic aggression. Catfish grown in total darkness, provided with refuges and living in turbid conditions were found to exhibit lowered cannibalistic and territorial aggression. Various lines of evidence suggested that cannibalism has a genetic basis, as was shown by the differences in cannibalistic behaviour of the two strains of Cyprinus carpio, viz. common and koi carp, with cannibalism being higher in the latter. This finding substantiates the hypothesis that cannibalism is genetically controlled and therefore open to evolutionary change. It is concluded that cannibalism is adaptive in times of food limitation, but that it is merely a by-product of normal feeding behaviour when food is abundant. Since cannibalism is advantageous and thus adaptive, the question arises ai to whether selection is occurring at the individual or the population level (or both). It was concluded that it is acting at the individual level, and that any benefits accruing at the population level iv were simply the effect of the initial cause, viz. individual selection. One of the aims of this study was to determine whether cannibalistic tendencies in fish are influenced by differing life history style trajectories. Based on the results of this study it is hypothesized that cannibalism is an r-selected trait. wi th the understanding gained from the knowl edge of the fundamental principles governing cannibalism, certain recommendations for its control in fish culture could be made. It is, however, imperative that further intensive studies be carried out to understand more fully this complex subject. General "rules" for regulating cannibalism could be helpful for any given cannibalistic species. However, in considering the differing life-history styles of each species, it becomes evident that species-specific guidelines need to be worked out. Until then, any suggestions for cannibalistic control offered to the aquaculturist can only serve as unrefined tools.
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- Date Issued: 1990
A study of possible interactions between the pineal gland and the opioidergic system
- Authors: Khan, Razeeya B
- Date: 1990
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3729 , http://hdl.handle.net/10962/d1001468
- Description: Recent observations suggest a link between the pineal gland and the opioid system. Possible areas of interaction between the pineal gland and the opioidergic system in Wistar rats were investigated. The effect of opioids on the pineal gland in organ culture was monitored. Neither morphine, methadone nor the opioid antagonist naloxone was found to affect [¹⁴C]-serotonin metabolism by the pineal gland in vitro. Both the pineal gland and the opioid system are influenced by exposure to stressful stimuli. Morphine and melatonin had protective effects on stress-induced gastric lesions. The ability of melatonin to inhibit lesion formation was found not to be exerted via an opioidergic mechanism. Evidence has been obtained for a possible modulation of the stress response by the pineal gland . The opioid drugs are the most potent analgesic agents available. A possible interaction between the opioid system and the pineal gland in the modulation of the response to noxious stimuli was investigated. An intact pineal gland was found to be necessary for the manifestation of the nocturnally increased response of rats to noxious stimuli
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- Date Issued: 1990
A study of real-time operating systems for microcomputers
- Authors: Wells, George Clifford
- Date: 1990
- Subjects: Operating systems (Computers) , Microcomputers
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4611 , http://hdl.handle.net/10962/d1004896 , Operating systems (Computers) , Microcomputers
- Description: This thesis describes the evaluation of four operating systems for microcomputers. The emphasis of the study is on the suitability of the operating systems for use in real-time applications, such as process control. The evaluation was performed in two sections. The first section was a quantitative assessment of the performance of the real-time features of the operating system. This was performed using benchmarks. The criteria for the benchmarks and their design are discussed. The second section was a qualitative assessment of the suitability of the operating systems for the development and implementation of real-time systems. This was assessed through the implementation of a small simulation of a manufacturing process and its associated control system. The simulation was designed using the Ward and Mellor real-time design method which was extended to handle the special case of a real-time simulation. The operating systems which were selected for the study covered a spectrum from general purpose operating systems to small, specialised real-time operating systems. From the quantitative assessment it emerged that QNX (from Quantum Software Systems) had the best overall performance. Qualitatively, UNIX was found to offer the best system development environment, but it does not have the performance and the characteristics required for real-time applications. This suggests that versions of UNIX that are adapted for real-time applications are worth careful consideration for use both as development systems and implementation systems.
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- Date Issued: 1990
A study of the effects of the pineal hormone, melatonin, on dopaminergic transmission in the central nervous system of rats
- Authors: Burton, Susan Frances
- Date: 1990
- Subjects: Dopaminergic mechanisms Melatonin Pineal gland -- Secretions Neural transmission Pineal gland Nervous system
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3726 , http://hdl.handle.net/10962/d1001463
- Description: Dopamine mechanisms in the central nervous system are important in the control of both normal and abnormal motor function. The recent observations in both animal and human studies, that melatonin, the principal hormone of the pineal gland, may have a role in the control of movement and the pathophysiology of movement disorders, have given rise to the concept that melatonin may have a modulatory influence on central dopaminergic neurotransmission. This study makes use of three animal behavioural models as well as a biochemical model of central dopaminergic function to further investigate the concept. Results from studies using the biochemical model, which investigated the effect of melatonin on dopamine and apomorphine stimulation of dopamine-sensitive adenylate cylase, suggest that melatonin is neither a competitive antagonist nor agonist at the D₁ receptor level, although the possibility of physiological stimulation or antagonism is not excluded. In behavioural studies, prior melatonin mg/kg administration (1 and 10 (8M) ip) inhibited apomorphine induced stereotypy and locomotor activity in normal rats, and apomorphine-induced rotational behaviour in 6-hydroxydopamine and quinolinic acid lesioned rats. The possibility that these results may have physiological significance is borne out by the observation that, under enviromental lighting conditions that are associated with raised endogeous melatonin levels, apomorphine- induced stereotypy and locomotor activity is attenuated. The general conclusion is that melatonin has an inhibitory influence on central nervous system dopaminergic function, suggesting therefore, that the pineal gland and melatonin may have a role in the pathophysiology and treatment of movement and behavioural disorders associated with dopaminergic dysfunction
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- Date Issued: 1990
An alternative peripheral executive for the data general AOS/VS operating system
- Authors: Tennant, Robert Satchwell
- Date: 1990
- Subjects: Operating systems (Computers) , Computers
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4566 , http://hdl.handle.net/10962/d1002031
- Full Text:
- Date Issued: 1990
An analysis of the early ontogeny of Aplocheilichthys johnstoni (Günter, 1893) from a life history perspective
- Authors: Haigh, Eliria H
- Date: 1990
- Subjects: Ontogeny -- History , Cyprinodontidae , Aplocheilidae , Fishes -- History
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5217 , http://hdl.handle.net/10962/d1005060
- Description: The reproductive and developmental styles of Aplocheilichthys johnstoni closely resemble those of other oviparous cyprinodont fishes reported in the literature. Reproductively it sorts to the guild of nonguarding phytophyllic broodhiders, is a daily, fractional spawner of relatively large, adhesive eggs. The length of the embryonic period varies between 14 and 19 days and the larval period can last for up to 30 days. Sexual maturity can be attained at an age of 150 days from fertilization. The embryology is described in detail and close comparison is made with the embryology of other cyprinodonts to highlight possible phylogenetic differences. Major differences with other cyprinodonts are in the rate of development, and heterochronic shifts in the appearance of certain structures in relation to each other. A discussion is included on the nature of development. It is suggested that development proceeds in a gradual stepwise fashion, interspersed with four major saltations, namely, fertilisation, onset of exogenous feeding, sexual maturity and death
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- Date Issued: 1990
An investigation into the nature and extent of erosion and sedimentation in the Maqalika Dam catchment, Maseru
- Authors: Ntsaba, 'Mankone 'Mabataung
- Date: 1990
- Subjects: Erosion -- Lesotho Sedimentation and deposition -- Lesotho Land use -- Lesotho -- History River sediments -- Lesotho
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4799 , http://hdl.handle.net/10962/d1001899
- Description: The study investigates the nature and extent of erosion and deposition in the Maqalika catchment, Maseru. Components of the study include (i) the re-construction of erosion and land use history in the catchment from 1961 to 1985, (li) determination of dominant factors or combinations of factors responsible for the observed erosion distribution at each date, (iii) the identification and evaluation of erosion and deposition features, and their spatial and temporal variations, and (iv) identification of socia-economic problems associated with observed erosion and deposition. Methods employed for the re-construction of erosion and land use history include the use of sequential aerial photography of 1961, 1979 and 1985, orthophoto maps and review of literature from past studies. It has been possible to map erosion and land use for the three time periods pinpointing areas of major change. Results are presented in map form showing the spatial distribution of each erosion class and each land use category. It was however not possible to derive any meaningful relationship between erosion distribution and land use, on the aforementioned maps. The only observation made from the comparison of the maps is that erosion degree and distribution sometimes changes with land use, while land use sometimes changes in response to erosion. Major land use changes are the conversion of agricultural land to urban land use, and grazing land. Due to the mnlti-dimensional nature of soil erosion, hand 'actor analysis was employed to determine which factors or combinations of factors were dominant at each date. Despite the extensive research on the various factors affecting erosion such as those used for the USLE and SLEMSA there is a growing uncertainty as to which factors are more important to erosion. Soil erodibility has been found to be a component of the major controlling factor combinations in all three periods under study. At each date erodibility combined with a number of other factors determined the observed erosion distribution. As suggested by Mosley (1980), Cambell (1985) and Coleman and Scatena (1986) sediment from a catchment is derived principally from spatially limited portions of the catchment. Likewise eroded sediment becomes deposited in spatially limited areas with special characteristics which encourage deposition. Aerial photographic survey aided by ground survey and oblique photographs were employed to identify sediment sources and sinks within the catchment. Some sediment sources are fIxed such as gully floors and sides, while some change location from time to time such as construction sites. An evaluation of portions of the catchment for their ability to supply and deliver sediment has shown that the most eroded areas are not the most active sediment sources. Sediment yield is limited by either supply or transport. Sediment yield was estimated using reservoir survey data which indicate that there is a high rate of soil loss from the catchment. One flaw of this method as a measure of soil loss is that it treats the measured sediment yield as if it were contributed uniformly from the basin. This method however affords the researcher to estimate minimum erosion rates, taking into account that large amounts of sediment are stored at various places within the catchment. The possible socio-economic consequences of erosion and deposition have been identified. These include loss of cropland, destruction of roads and building sites which require methods of reclamation, sedimentation of small reservoirs and ponds, and the formation of gully bottom fills which are potential sediment sources. Conservation measures presently applied in the catchment are assessed and found to be irrelavant to the present erosion problem. Data from the reservoir survey revealed that the estimated rate of soil loss is more important to on-site erosion damage than to off-site damage in the form of the sedimentation of Maqalika reservoir. Appropriate conservation measures such as those suggested by Amimoto (1981) would be relevant to the study area, however the main constraint in their implementation would be lack of legislation and the absence of a sound land use policy. It is therefore concluded that the present land use situation which does not take into consideration the physical constraints of the catchment is partly responsible for accelarated erosion in the catchment.
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- Date Issued: 1990
Immunolocalisation of, and ultrastructural changes in the LH gonadotropes of Miniopterus schreibersii and Rhinolophus capensis (Mammalia: Chiroptera) in relation to their reproductive cycles
- Authors: Bojarski, Christina
- Date: 1990
- Subjects: Bats -- Reproduction
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5589 , http://hdl.handle.net/10962/d1002039
- Description: LH gonadotropes are successfully identified in the anterior pituitary of Minioptems schreibersii and Rhinolophus capensis, using immunogold labelling at the light and electron microscopy level. The gonadotropes are oval to polygonal in shape and possess numerous small secretory granules, which contain LH throughout the year. Their distribution is similar in both species, although the abundance varies slightly between species and sexes. Seasonal changes are detected in gonadotrope ultrastructure, pituitary LH levels, and plasma hormone levels, and activity pattern of LH gonadotropes generally coincide with reproductive activity in both bat species. In female M. schreibersii gonadotrope activity, pituitary LH, and plasma LH levels coincide with development, implantation and gestation. During the delayed implantation gonadotropes are inactive and and high follicular period of plasma LH levels are low, coinciding with corpus luteum inactivity and low plasma progesterone levels. Implantation coincides with increased activity of the gonadotrope activity, increased plasma LH levels, reactivation of the corpus luteum and elevated plasma progesterone levels. Activation of LH gonadotropes towards the end of hibernation may be initiated by the winter solstice, which marks the change to increasing daylength. In female R capensis gonadotrope activity and high pituitary and plasma LH levels occur towards the end of follicular development. During hibernation gonadotrope activity and plasma LH levels decrease. Ovulation coincides with increasing gonadotrope activity (which follows the winter solstice), although a preovulatory peak in plasma LH is not detected. In male M. schreibersii and R. capensis, gonadotrope activity and high plasma LH and testosterone levels coincide with spermatogenesis, except during a period of reproductive inactivity in spring in male M. schreibersii, where gonadotropes appear active and plasma LH and testosterone levels are high. The reason for this apparent activity is not known. Male sperm storage during hibernation in male R. capensis coincides with low gonadotrope activity and low plasma LH and testosterone levels. Factors initiating gonadotrope activity and hence spermatogenesis are probably decreasing daylength (summer solstice) in M. schreibersii and increasing ambient temperatures and food abundance (following the hibernation period) in R.capensis.
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- Date Issued: 1990
Initial findings of an investigation into the feasibility of a low level image processing workstation using transputers
- Authors: Cooke, Nicholas Duncan
- Date: 1990 , 2013-02-07
- Subjects: Image processing , Computer graphics , Fourier transformations -- Data processing , Transputers
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4679 , http://hdl.handle.net/10962/d1006702 , Image processing , Computer graphics , Fourier transformations -- Data processing , Transputers
- Description: From Introduction: The research concentrates primarily on a feasibility study involving the setting up of an image processing workstation. As broad as this statement concerning the workstation may seem, there are several factors limiting the extent of the research. This project is not concerned with the design and implementation of a fully-fledged image processing workstation. Rather, it concerns an initial feasibility study of such a workstation, centered on the theme image processing aided by the parallel processing paradigm. In looking at the hardware available for the project, in the context of an image processing environment, a large amount of initial investigation was required prior to that concerned with the transputer and parallel processing. Work was done on the capturing and displaying of images. This formed a vital part of the project. Furthermore, considering that a new architecture was being used as the work horse within a conventional host architecture, the INTEL 80286, several aspects of the host architecture had also to be investigated. These included the actual processing capabilities of the host, the capturing and storing of the images on the host, and most importantly, the interface between the host and the transputer [C0089]. Benchmarking was important in order for good conclusions to be drawn about the viability of the two types of hardware used, both individually and together. On the subject of the transputer as the workhorse, there were several areas whlch required investigation. Initial work had to cover the choice of network topology on whlch the benchmarking of some of the image processing applications were performed. Research into this was based on the previous work of several authors, whlch introduced features relevant to this investigation. The network used for this investigation was chosen to be generally applicable to a broad spectrum of applications in image processing. It was not chosen for its applicability for a single dedicated application, as has been the case for much of the past research performed in image processing [SAN88] [SCH89]. The concept of image processing techniques being implemented on the transputer required careful consideration in respect of what should be implemented. Image processing is not a new subject, and it encompasses a large spectrum of applications. The transputer, with image processing being hlghly suited to it, has attracted a good deal of research. It would not be rash to say that the easy research was covered first. The more trivial operations in image processing, requiring matrix type operations on the pixels attracted, the most coverage. Several researchers in the field of image processing on the transputer have broken the back of this set of problems. Conclusions regarding these operations on the transputer returned a fairly standard answer. An area of image processing which has not produced the same volume of return as that concerning the more trivial operations, is the subject of Fourier Analysis, that is, the Fourier Transform. Thus a major part of this project concerns an investigation into the Fourier Transform in image processing, in particular the Fast Fourier Transform. The network chosen for thls research has placed some constraint upon the degree of parallelism that can be achleved. It should be emphasized that this project is not concerned with the most efficient implementation of a specific image processing algorithm on a dedicated topology. Rather, it looks at the feasibility of a general system in the domain of image processing, concerned with a hlghly computationally intensive operation. This has had the effect of testing the processing power of the hardware used, and contributing a widely applicable parallel algorithm for use in Fourier Analysis. 3 These are discussed more fully in Chapter 2, which covers the work related to tbis project. The results of the investigation are presented along with a discussion of the methods throughout the thesis. The final chapter summarizes the findings of the research, assesses the value of the investigation, and points out areas for future investigation.
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- Date Issued: 1990
Interactions between fig wasps and their host figs
- Authors: Nefdt, Rory John Charlton
- Date: 1990
- Subjects: Wasps , Mutualism (Biology)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5718 , http://hdl.handle.net/10962/d1005404 , Wasps , Mutualism (Biology)
- Description: Fig trees (Ficus spp.) and fig wasps (Hymenoptera: Agaonidae) are partners in an intimate mutualism. The trees provide ovules in which wasp larvae develop while the wasps pollinate the flowers and are therefore indispensible for fig seed production. Agaonid fig wasps oviposit down the styles of fig flowers and it has generally been accepted that they were unable to reach the ovules of "long" styled flowers , which would produce seeds, thus maintaining an evolutionary stable mutualism. African fig species were found to have unimodal style length frequencies, with no separation into long and short styled flowers. In several species the ovipositors of their associated agaonids were long enough to reach the majority of ovules. The number of foundress agaonids entering a fig influenced fig seed set and therefore was an important factor regulating the proportion of flowers producing seeds or pollinators. In the two Ficus species that were studied, entry of more than three agaonid foundresses into one fig resulted in competition for limited oviposition sites and less female - biased offspring sex ratios. It is hypothesised that sequential laying of male eggs followed by female eggs, under variable oviposition site limitation, results in sex ratio adjustment, as predicted by local mate competition theory. Evidence in support of this hypothesis is presented. A number of non - pollinating torymid and pteromalid fig wasps also oviposit into each fig species. The sycophagines and sycoecines oviposit down the styles from inside the fig inflorescences like their agaonid counterparts, while other species insert their ovipositors through the wall of the fig from the outside. Like the agaonids, sycophagines were characterised by being pro - ovigenic, with numerous fully developed eggs at emergence. Sycoecines were able to re - emerge from figs they had oviposited in and lay their eggs in more than one fig. They had short ovipositors, allowing access to a smaller proportion of flowers than agaonids or sycophagines. Externally ovipositing fig wasps were syn-ovigenic, able to develop eggs as adults and invested more energy and time during each oviposition event. Differences in the ovipositor lengths of these species did not segregate their oviposition sites spatially, and therefore does not reduce competition between species. Attack by parasitoids and inquiline fig wasps from the exterior did not constitute a selection pressure against agaonids ovipositing in ovules closer to the periphery of the fig's surface, as predicted by Michaloud's enemy-free-space hypothes is. It cannot therefore explain the preference shown by ovipositing agaonids for shorter styled flowers.
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- Date Issued: 1990
Sand inundation on rocky shores : its effects on species richness and the structure of species assemblages
- Authors: Dower, Katherine Mary
- Date: 1990
- Subjects: Intertidal ecology , Tide pool ecology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5814 , http://hdl.handle.net/10962/d1007183 , Intertidal ecology , Tide pool ecology
- Description: Although sand deposits are present on many intertidal rocky shores, their effects on species richness, zonation and trophic structure have often been overlooked. This study is the first to recognise sand as an important abiotic factor on South African rocky shores. Rocky shores in the eastern Cape Province of South Africa are subject to extensive sand inundation and are composed of two hard substrata of differing topographies. Four sites on one substratum and six on the other were sampled quantitatively using quadrats. The biota were identified, counted and/ or weighed to provide a matrix of species biomass and numbers in separate zones. This matrix was then analysed using ordination and classification. A total of 321 species were identified which is more than local rocky or sandy shores. While the intermediate disturbance hypothesis would predict high species richness on these shores, it does not fully explain this richness nor the distribution of species assemblages. Habitat heterogeneity, including the dynamics of sand deposits, is strongly influenced by substratum topography and is the most important factor generating species richness. Abrasion by sand (sand scour) causes local reductions in richness but the presence of semi-permanent sand deposits allows habitation by psammophilic and sand-dependent species. As a result the biota of a sand inundated rocky shore includes both a full rocky shore and a large sandy beach component. Substratum topography controls patterns of sand deposition and retention and community analysis showed that samples were clustered primarily according to species richness and secondarily according to substratum type. Ordination of species identified an arc of species assemblages of decreasing levels of sand tolerance. These corresponded to sample groupings so that th assemblages found in various habitats were characterised by particular levels of sand tolerance. The presence of sand has a negative effect on the biomass of primary producers and filter feeders but a positive effect on the biomass of deposit feeders. Because sand is retained to different degrees in different zones, trophic structure varies between zones and to a lesser extent, between rock types. In general, however, the trophic structure of sand inundated rocky shores is similar to that of non-inundated shores.
- Full Text:
- Date Issued: 1990
Synthetic and spectrometric investigation of 1,4-benzoxazepines
- Authors: Whittal, R D
- Date: 1990
- Subjects: Spectrum analysis , Benzodiazepines
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4381 , http://hdl.handle.net/10962/d1005046
- Description: Flavanone (2,3-dihydro-2-phenyl-4H-benzopyran-4-one) and a series of 4'- and 7-halogeno derivatives were prepared from the corresponding 2'-hydroxychalcones [1-(2-hydroxyphenyl)-3-phenyl-2-propen-l-ones], which, in turn, were synthesized by aldol condensation of substituted 2'-hydroxacetophenones with various benzaldehydes. A series of 2,3-dihydro-2-phenyl-l,4-benzoxazepin-5(4H)-ones were prepared by ring expansion of the corresponding flavanones, via the Schmidt reaction, using trimethylsilylazide and trifluoroacetic acid. A series of tetrazoles {2,3-dihydro-2-phenyl-tetrazolo[1,5-d]-1,4-benzoxazepines} were also isolated as by-products of the Schmidt reaction. Flavanone oxime was synthesized for use in Beckmann reactions, and its molecular structure was determined by x-ray crystallography. Attempts to prepare 1,4-benzoxazepinone or its 1,5-analogue via Beckmann rearrangement of flavanone oxime, with polyphosphoric acid or phosphorus pentachloride catalysts, however, were unsuccessful. Several methods for introducing Δ²-unsaturation into the benzoxazepinone system were also examined. High resolution ¹H n.m.r., computer modelling, and molecular mechanics techniques were used to determine the conformations of the heterocycles of the benzoxazepinones and tetrazoles and results are compared with earlier studies in this field. Certain trends in the fragmentation patterns were observed in the low resolution mass spectra of the benzoxazepinones and tetrazoles, and high resolution mass spectrometric data were used to explore the major fragmentation patterns of these compounds.
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- Date Issued: 1990
The applicability of two simple single event rainfall-runoff models to catchments with different climate and physiography
- Authors: Beater, Anne Brenda
- Date: 1990
- Subjects: Hydrogeology Runoff -- South Africa Runoff -- United States Rain and rainfall -- South Africa Rain and rainfall -- United States
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4796 , http://hdl.handle.net/10962/d1001896
- Description: The study presents the results of applying two isolated event, constant runoff proportion, conceptual models to a range of catchments drawn from various climatic and physiographic regions of South Africa and the USA. The models can be operated in either lumped or semi-distributed modes. The research progressed through the following stages. The initial stage involved the calibration of both models on two sets of catchments so that an initial evaluation of the performance of the models could be carried out and any deficiencies in the model structure identified, and where practical, corrected. The models were then calibrated on a further 8 catchments. An important result of the calibration is that for both models to produce reasonably acceptable simulations, at least one parameter has to vary between storms on the same catchment to account for variations in storm or antecedent moisture characteristics. The next stage consisted of compiling quantitative descriptions of the physical characteristics of the catchments and rainfall events and an attempt to relate the calibrated parameter values to relevant physical characteristics for the purpose of estimating parameter values when calibration is not possible. Despite the difficulties encountered in quantifying some of the hydrological characteristics the general trends exhibited by many of the relationships are encouraging and the format of the combinations of physical variables used, do make sense with respect to the original parameter conceptualisations. The relationships between storm characteristics and parameters of both models are less satisfactory. There is a high degree of scatter and the between-catchment variation in the form of the relationships, indicates that the derived relationships are likely to be of little use for parameter estimation purposes. The final stage involved a validation exercise in which new parameters were estimated from the physical variable-parameter relationships for all the catchments previously used, as well as a further four. The new parameters were used to re-simulate all the storms and comparison of these results were made with the original calibration results. Both models produced poor results and are unlikely to give reliable results where calibration is not possible. The parameter relationships for the parameters related to storm characteristics are so catchment specific that transfer to other areas will produce unpredictable results. Foot note:- For compatability with computer printouts decimal full stops are used in the format of real numbers in tables etc
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- Date Issued: 1990
The geology and alteration-mineralisation of the Gamigab Tin Prospect, Damaraland, Namibia
- Authors: Walraven, Felix Caspar
- Date: 1990
- Subjects: Mineralogy -- Namibia -- Damaraland , Geology -- Namibia -- Damaraland , Tin mines and mining -- Namibia -- Damaraland
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4956 , http://hdl.handle.net/10962/d1005568 , Mineralogy -- Namibia -- Damaraland , Geology -- Namibia -- Damaraland , Tin mines and mining -- Namibia -- Damaraland
- Description: The stratigraphy at the Gamigab Sn prospect consists of two mainly schistose units separated by a thick marble unit which have been assigned to the Orusewa, the Karibib and the Kuiseb Formations respectively. Four phases of folding affected the lithologies with the south-south-west trending F2 folds defining the main structures in the region. The area underwent low grades of metamorphism. Temperatures were in the range 420° to 500°C and pressures less than 2 kbars. The effects of contact metamorphism are seen in the south-east and south-west. Regional metamorphism outlasted the deformation and contact metamorphism started late during deformation. Two Karoo-age intrusions penetrated the metasediments north of the mineralisation. One is an altered porphyry plug and the other is a weathered dolerite plug, the latter containing xenoliths of undeformed Karoo sediments. Cassiterite is hosted within east-west trending quartz veins that cross-cut previously altered schistose country rocks. The alteration types include sericitisation, tourmalinisation, carbonatisation and ferruginisation. Preliminary Rb/Sr dating on muscovite from the alteration zone suggests an age of 509 ± 11 Ma. Breccias of probable hydrothermal origin are spatially associated with the mineralisation. These hydraulic breccias occur in antiformal structures within the marble and developed in response to a sudden pressure release due to a build up of fluids at the contact between the schistose Orusewa and carbonate Karibib Formations.
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- Date Issued: 1990
The stock identification of the Cape horse mackerel, Trachurus trachurus capensis (Pisces: Carangidae)
- Authors: Naish, Kerry-Ann
- Date: 1990
- Subjects: Carangidae -- Identification , Trachurus trachurus -- Identification , Carangidae -- South Africa , Trachurus trachurus -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5320 , http://hdl.handle.net/10962/d1005165 , Carangidae -- Identification , Trachurus trachurus -- Identification , Carangidae -- South Africa , Trachurus trachurus -- South Africa
- Description: Three stocks of the Cape horse mackerel, Trachurus trachurus capensis, have been recognised in the ICSEAF convention region off southern Africa (Figure 1) on the basis of catch distribution and biological analyses. They were identified as being off Namibia (ICSEAF Divisions 1 . 3 and 1.4), off the West Coast of South Africa (Div. 1.6) and off the South Coast (Div. 2.1 and 2.2). Recently, speculation as to the accuracy of the classification of the South African populations has arisen. The aim of this study was to determine the number of stocks In Divisions 1.6, 2.1 and 2.2, using methodology which assessed the spatial and temporal nature and the phenotypic and genotypic characteristics of the species. The distribution of horse mackerel was evaluated by studying the trends in catch data and length frequency distributions obtained from the demersal industry during 1986 to 1988 and from the demersal biomass cruises in 1987 and 1988. Adult fish, found in Div. 2.1 and 2.2, probably migrated; during the months in which horse mackerel were expected to spawn, CPUE values were higher over the central Agulhas Bank than in the east of the study region. During the quiescent period, catches and numbers were higher in the latter region. Juvenile horse mackerel were found in the nursery areas utilised by pelagic fish and it is likely that they were transported northward from the Agulhas Bank. A decrease in biomass of individuals in Div. 1.5 indicated a separation between a northern and a southern population. Analyses of the phenotype, or epigenetic characters, of horse mackerel were used as a further test of stock integrity. Monthly samples were drawn from Div. 1.6, 2.1 and 2.2 during May 1988 to June, 1989. Comparisons for the values obtained from growth, length-at-50%-maturity and the season of otolith ring formation from each region showed no significant variation in the phenotype. Morphometric analysis proved inconclusive. Monthly gonadosomatic indices show that fish south of the Orange River share the same spawning season. Reports from the literature show that horse mackerel in the latter region differ from those of northern Namibia in spawning season, age-at-50%-maturity and season of otolith ring formation. The genetic structure of the populations of horse mackerel was evaluated by means of a restriction enzyme analysis of the Mitochondrial DNA of 37 fish collected from Divisions 1.4, 1.5, 1.6, 2.1 and 2.2. Two composite genotypes of horse mackerel were found; one belonging to fish in Div. 1.4 and one to fish in Div. 1.6, 2.1 and 2.2. The genetic distance between the two genotypes, 0.07, was the expected distance between two populations at a subspecies level. Fish in Div. 1.5 consisted of both genotypes and may be a region of mixing between the two populations. The interpretation of results was cautioned; prevlous work has shown that the migration of a small number of individuals between two stocks can be expected to maintain a low variation between the populations. In concluding, it was recommended that the horse mackerel be managed as two stocks, one in Div. 1.3 and 1.4 and one in Div. 1.6, 2.1 and 2.2
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- Date Issued: 1990
The taxonomy and life history of the blue stingray, Dasyatis marmorata capensis (Batoidea : Dasyatidae) from Southern Africa
- Authors: Cowley, Paul D (Paul Denfer), 1964-
- Date: 1990
- Subjects: Stingrays -- Africa, Southern Dasyatidae
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5183 , http://hdl.handle.net/10962/d1001959
- Description: The blue stingray, Dasyatis marmorata capensis is an important recreational shore angling species, especially amongst tournament anglers, in South Africa. The taxonomy and aspects of the biology of this species were investigated from specimens collected between March 1987 and May 1989, from various localities along the southern African coastline. Morphological comparisons were made between Dasyatis pastinaca, Dasyatis marmorata and the local variety. The results indicated that the local variety is different to D. pastinaca in colouration, disc length size, and vertebral and radial counts. The proposed new sub-species Dasyatis marmorata capensis is closest to Dasyatis marmorata of the north-eastern Atlantic coast, but differs in snout angle, disc length and snout to vent length. Analysis of catch per unit of effort data revealed that the blue stingray exhibits distinct seasonality. Adult and sub-adult fish undertake an annual onshore/offshore migration and invade the inshore region between spring and summer, while juveniles remain offshore and utilize this zone as a nursery area. Length-at-age and growth rates for both sexes were estimated from bands formed seasonally in the vertebral centra. The derived von Bertalanffy growth parameters indicated that females reach a greater asymptotic size (913.8 mm DW) and have a slower growth rate (K = -0.07) than males (531.8 mm DW, K = -0.17). Growth rates of juvenile captive specimens compared favourably with back calculated values, at approximately 65 mm/year, for the first year of growth. Dasyatis marmorata capensis is an aplacental viviparous elasmobranch with a well defined annual breeding cycle. Young are released , at a size of 170-200 mm DW, from October to December after a gestation period of approximately nine months. An average fecundity of 3.07 was calculated . Examination of gonadal development indicated that sexual maturity is first attained at a size of 408 and 500 mm DW in males and females, respectively, corresponding to ages 4.5 and 7 years. Size-related food habits were determined independently from three depth zones. The major prey items for each size class were directly related to the abundance of the infauna of the different depth zones. Polychaeta, amphipoda and stomatopoda were the major prey items in the offshore zone. In the nearshore zone, the dominant prey species were Balanoglossus capensis and Callianassa spp .. The high incidence of empty stomachs and the presence of prey items which occur in greatest abundance beyond the surf zone indicated that this zone is not utilized for the purposes of feeding.
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- Date Issued: 1990