Knockdown of Hop downregulates RhoC expression, and decreases pseudopodia formation and migration in cancer cell lines:
- Authors: Willmer, Tarryn , Contu, Lara , Blatch, Gregory L , Edkins, Adrienne L
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/165196 , vital:41217 , DOI: 10.1016/j.canlet.2012.09.021
- Description: The Hsp90/Hsp70 organising protein (Hop) is a co-chaperone that mediates the interaction of Hsp90 and Hsp70 molecular chaperones during assembly of Hsp90 complexes in cells. Formation of Hsp90 complexes is a key intermediate step in the maturation and homeostasis of oncoproteins and several hormone receptors. In this paper, we demonstrate that knockdown of Hop decreased migration of Hs578T and MDA-MB-231 breast cancer cells. Hop was identified in isolated pseudopodia fractions; it colocalised with actin in lamellipodia, and co-sedimented with purified actin in vitro. Knockdown of Hop caused a decrease in the level of RhoC GTPase, and significantly inhibited pseudopodia formation in Hs578T cells. Our data suggest that Hop regulates directional cell migration by multiple unknown mechanisms.
- Full Text:
- Date Issued: 2013
Land claims and the pursuit of co-management on four protected areas in South Africa
- Authors: Cundill, Georgina , Thondhlana, Gladman , Sisitka, Lawrence , Shackleton, Sheona , Blorea, M
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/391223 , vital:68632 , xlink:href="https://doi.org/10.1016/j.landusepol.2013.05.016"
- Description: Successful land claims on protected areas by previously disenfranchised communities often result in co-management agreements between claimant communities and state conservation agencies. South Africa, in particular, has pursued co-management as the desired outcome of land claims on its protected areas. We review four cases of co-management on protected areas in South Africa, and reflect on the appropriateness of the pursuit of co-management as the preferred outcome of land claims. Despite promises of pro-poor, democratically informed management, the practical experience of co-management has seen the continuation of the status quo in terms of conservation, with very few material benefits for claimant communities and limited sharing of responsibilities and decision-making functions. The findings underscore two deep challenges facing co-management in cases of land claims worldwide. First, during land claims negotiations in cases involving protected areas, the state cannot be expected to represent the best interests of its citizens (the land claimants), while simultaneously seeking to meet national and international obligations for protected area coverage. Second, the concept of democratic co-management may sit uncomfortably beside the realities of managing loss-making protected areas with ever-shrinking conservation budgets. Where co-management agreements have already been signed, ensuring that new landowners do indeed have a say in management should form the driving focus for co-management practice going forward.
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- Date Issued: 2013
Lechwe Trust Collection:
- Authors: Simbao, Ruth K
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/147547 , vital:38648 , https://doi.org/10.1162/afar.2005.38.3.78
- Description: The caustic humor of The Arts Delegate (2000), by the cartoonist popularly known in Zambia as" Yuss," captures a dynamic readily found in the Zambian art world.
- Full Text:
- Date Issued: 2013
Like Katherine
- Authors: Morgan, Jane Mary Kathleen
- Date: 2013
- Subjects: Creative writing , Fiction , South Africa , Creative writing (Higher education) , Short stories, South African (English) -- 21st century , South African fiction (English) -- 21st century , English language -- Writing
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: vital:5964 , http://hdl.handle.net/10962/d1001814
- Description: Vicky, a thirty something English radio journalist, has moved to Cape Town to try and work out what it is that's missing from her life and to fill the gap. At first she thinks she's found what she's looking for, but a series of unsettling events makes her realise she has simply brought her problems with her. She goes back to England, ostensibly for work, where she is contacted by her stepbrother, Mark. They hardly know each other but he has a reason for wanting to find her. They meet and, for both of them, their encounters change the way they see themselves and their relationships. Vicky comes to understand more about her past and her family and, for the first time, to find a connection with her emotional life
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- Date Issued: 2013
Linguistic minorities in the South African context : the case of Tshivenda
- Authors: Luvhengo, Nkhangweleni
- Date: 2013
- Subjects: Indigenous African languages , Apartheid , Tshivenda , Minority languages , Development , Status , Multilingualism , Sesotho , isiZulu , Perceptions , Linguistic minorities -- Research -- South Africa , Venda language -- Research -- South Africa , Language policy -- Research -- South Africa , Multilingualism -- Research -- South Africa , Linguistic rights -- Research -- South Africa , Language and languages -- Political aspects
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: vital:3558 , http://hdl.handle.net/10962/d1001862
- Description: After many years of the oppressive apartheid government, the new democratic era came into being in 1994. Lot of policy changes came into being, including language policy. This new language policy of the post-apartheid era recognises eleven official languages which include the nine indigenous African languages which were previously recognised as regional languages in the different homelands. The present study investigates the progress of Tshivenda in terms of status and development since it was accorded the official status in South Africa. Literature investigating the status of Tshivenda is generally sparse. This study investigates the status of Tshivenda in South Africa to explore how minority languages which are also recognised as official languages are treated. In most multilingual countries, there are issues which affect the development of minority languages, but the South African situation is interesting in that some of the minority languages are recognised as official languages. This study is a comparative in nature. Firstly, the study compares the level of corpus planning and development in Tshivenda and other indigenous South African languages. Secondly, it compares how people use Tshivenda in a rural area of Lukalo Village where the language is not under pressure from other languages and in Cosmo City, an urban area in Gauteng where Tshivenda speakers come into contact with speakers of more dominant languages such as isiZulu and Sesotho. Language use in different domains like, media, education, government and the home is considered in order to establish how people use languages and the factors which influence their linguistic behaviours. The study also establishes the perceptions and attitudes of the speakers of Tshivenda as a minority and those of the speakers of other languages towards Tshivenda’s role in the different domains such as education and the media. This study was influenced by previous research (Alexander 1989, Webb 2002) which found out that during the apartheid period Tshivenda speakers used to disguise their identity by adopting dominant languages like isiZulu and Sesotho in Johannesburg. Accordingly, the present research wanted to establish how the language policy change in the democratic era has impacted on the confidence of Tshivenda speakers regarding themselves and their language. This study establishes that although Tshivenda is now an official language in post-apartheid South Africa, it still has features of underdevelopment and marginalization that are typically of unofficial minority languages. Translation, lexicographic and terminological work in this language still lags behind that of other indigenous South African languages and there is still a shortage of school textbooks and adult literature in this language. As a result, using the language in education, the media and other controlling domains is still quite challenging, although positive developments such as the teaching of the language at university level can be noted. The Tshivenda speakers generally have a positive attitude towards their language and seem prepared to learn and use it confidently as long its functional value is enhanced, which is currently not happening. As a result, some Tshivenda speakers still regard English as a more worthwhile language to learn at the expense of their language
- Full Text:
- Date Issued: 2013
Local setting influences the quantity of household food waste in mid-sized South African towns
- Authors: Chakona, Gamuchirai , Shackleton, Charlie M
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/67289 , vital:29067 , https://doi.org/10.1371/journal.pone.0189407
- Description: publisher version , From Introduction: The world faces a food security challenge with approximately 868 million people undernourished and about two billion people suffering from the negative health consequences of micronutrient deficiencies [1]. Yet, at least one-third of food produced for human consumption is lost or wasted along the food chain between farm and fork [2–7]. Food waste refers to wholesome edible material intended for human consumption, arising at any point in the food supply chain that is instead discarded, lost, degraded or consumed by pests [8]. Food loss is defined as the decrease in food quantity or quality which makes it unfit for human consumption [9]. According to the European Commission [10], food waste is composed of raw or cooked food materials such as vegetable peelings, meat trimmings and spoiled or excess ingredients or prepared food as well as bones, carcasses and organs. However, food waste can be measured only for edible products that are directed to human consumption [6]. Food losses take place at production, postharvest and processing stages in the food supply chain and the food losses that occur at the end of the food chain (retail and final consumption) are called “food waste” [11]. In our study food waste refers to food losses that occur at the end of the food chain (final consumption at household or consumer level) which include edible products that are directed to human consumption and are discarded when not consumed for various reasons. It is related to consumers’ behaviour [6,11] and it includes food loss before, during or after meal preparation in the household. , The research was funded by VW foundation under the Livelihoods Urbanisation and Natural Resources in Africa (LUNA) project through Freiburg University, Germany. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of this manuscript
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- Date Issued: 2013
Making meaning of citizenship: how ‘born frees’ use media in South Africa's democratic evolution
- Authors: Malila, Vanessa , Oeofsen, Marietjie , Garman, Anthea
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/159790 , vital:40344 , DOI: 10.1080/02500167.2013.852598
- Description: By examining young people's habits of using the media in relation to citizenship, this article responds to calls that the starting point for research into citizenship and democracy should be the perspectives of citizens themselves. Drawing on both quantitative and qualitative research with young South Africans (the ‘born free’ generation), the study sought to gain insight into how young people use media to make sense of notions of citizenship and participatory democracy in ways that are relevant and reliable to their everyday lives.
- Full Text:
- Date Issued: 2013
MCD spectroscopy and TD-DFT calculations of a naphthalene-ring-bridged coplanar binuclear phthalocyanine dimer
- Authors: Mack, John , Liang, Xu , Dubinina, Tatiana V , Tomilova, Larisa G , Nyokong, Tebello , Kobayashi, Nagao
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/232511 , vital:49998 , xlink:href="https://doi.org/10.1142/S1088424613500259"
- Description: Magnetic circular dichroism (MCD) spectroscopy and TD-DFT calculations are used to analyze the electronic structure and optical properties of an alkyl-substituted naphthalene-ring-bridged coplanar binuclear phthalocyanine dimer. An analysis of the MCD spectrum of the naphthalene-ring-bridged dimer relative to those of benzene-bridged compounds reported previously, demonstrates that there is a significantly weaker interaction between the two phthalocyanine rings. TD-DFT results obtained using the B3LYP functional with 6–31G basis sets were found to be problematic. Closer agreement with the experimental data is obtained when the CAM-B3LYP functional is used instead. The naphthalene-ring-bridged compound is found to be unsuitable for use as a photosensitizer for the formation of singlet oxygen, because the ΦT values are negligible.
- Full Text:
- Date Issued: 2013
Media, citizenship and the politics of belonging in contemporary South Africa:
- Authors: Milton, Viola C , Wasserman, Herman , Garman, Anthea
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/159802 , vital:40345 , DOI: 10.1080/02500167.2013.864447
- Description: Drawing on a wide range of theoretical and empirical studies, the articles in this special issue examine issues of citizenship and belonging in South Africa. Questions of belonging and citizenship are neither novel, nor particular to South Africa – they have been high on the intellectual (and popular) agenda internationally since at least the early 1990s. Yet South Africa's history of artificially separating and defining its citizens in the racial regimes of colonialism and apartheid still reverberates today, as is reflected in the continued inequalities marring South African society
- Full Text:
- Date Issued: 2013
Metal nanoparticles caused death of metastatic MDA-MB-231 breast cancer cells:
- Authors: Adeyemi, Oluyomi , Edkins, Adrienne L , Whiteley, Christopher
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/164797 , vital:41173 , DOI: 10.1016/j.toxlet.2013.05.599
- Description: Available data on the toxicity of nanoparticles is a subject of controversy. The interaction of nanoparticles with biological systems including living cells has become one of the most urgent areas of collaborative research in materials science and biology. This is due to the fact that toxicity of nanomaterials are ill defined in terms of cause–effect relationships.
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- Date Issued: 2013
Mg/Triethylammonium Formate: A Useful System for Reductive Dimerization of Araldehydes into Pinacols;Nitroarenes into Azoarenes and Azoarenes into Hydrazoarenes
- Authors: Pamar, M Geeter , Govender, P , Muthusamy, K , Krause, Rui W M
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125107 , vital:35729 , https://doi.org/10.13005/ojc/290316
- Description: Studies are reported which describes the effectiveness of triethylammonium formate in the presence of magnesium for the efficient intermolecular pinacol coupling using MeOH as solvent. Various aromatic carbonyls underwent smooth reductive coupling to give the corresponding 1,2-diols. A series of azo compounds were obtained by the reductive coupling of nitroaromatics while azo compounds were reduced to the corresponding hydrazoarenes by this system. There was no adverse effect on the other reducible and hydrogenolysable groups such as ether linkage, hydroxy and halogens. The reactions are clean, high yielding and inexpensive. All the reactions proceeded smoothly at ambient temperature.
- Full Text:
- Date Issued: 2013
Microbial agents for control of aquatic weeds and their role in integrated management
- Authors: Ray, P , Hill, Martin P
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/423550 , vital:72071 , xlink:href="https://www.cabidigitallibrary.org/doi/abs/10.1079/PAVSNNR20128014"
- Description: Aquatic ecosystems throughout the world are threatened by the presence of invasive aquatic plants, both floating and submerged. Some of the aquatic species, such as water hyacinth (Eichhornia crassipes [Mart.] Solms), alligator weed, Alternanthera philoxeroides (Mart.), giant salvinia, Salvinia molesta D.S. Mitchell and water lettuce (Pistia stratiotes L.), Griseb. despite being relatively minor problems in their native range, have become major invaders of aquatic habitats in other parts of the world after having escaped from their natural enemies. Unchecked growth of aquatic vegetation is generally undesirable and reduces the value of the water resource. Despite adopting all control options including manual, mechanical, chemical and classical biological, the problem persists. The current weed management is oriented towards finding approaches that are effective in controlling the weed and reducing environmental contamination from herbicides. Plant pathogens have been gaining increasing attention and interest among those concerned with developing environmentally friendly, effective and compatible approaches for integrated management of the noxious weeds. This paper discusses some of the major microbial agents associated with aquatic weeds and their increasing role in integrated weed management.
- Full Text:
- Date Issued: 2013
Modeling Complex Networked Audio Devices
- Authors: Eales, Andrew , Foss, Richard
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/427012 , vital:72408 , https://www.aes.org/e-lib/browse.cfm?elib=16706
- Description: The lack of a universally accepted standard control protocol, as well as a lack of interoperability among existing protocols, limits how audio software can support a wide range of networked devices. To address this issue, the authors propose an abstract standard audio device model that is independent of any particular technology. This standard device model supports service discovery and enumeration, while also specifying device parts, the representation of parameter addresses, and the visual appearance of controls. The discussion explores the model in the context of a self-configuring discovery and control environment. Within this environment, controllers do not “pull” descriptions from a device; but rather, devices “push” full-service implementations to controllers.
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- Date Issued: 2013
Morphological and genetic characterization of a South African Plutella xylostella granulovirus (plxy GV) isolate
- Authors: Abdulkadir, Fatima , Marsberg, Tamryn , Knox, Caroline M , Hill, Martin P , Moore, Sean D
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/406117 , vital:70240 , xlink:href="https://hdl.handle.net/10520/EJC132828"
- Description: Plutella xylostella (L.) (Lepidoptera: Plutellidae), also known as diamondback moth, is a destructive insect pest of cruciferous crops (Talekar and Shelton 1993; Shelton 2004). The pest occurs wherever its host plants are cultivated and the global annual cost of damage and control is estimated to be US$4-5 billion (Zalucki et al. 2012). The extensive use of synthetic pesticides for control combined with the high fecundity of P. xylostella has resulted in the pest developing resistance to nearly all classes of insecticides (Grzywacz et al. 2009). Moreover, these chemicals have negative environmental implications and may affect non-target species, some of which are natural enemies of the pest.
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- Date Issued: 2013
Movement patterns and habitat selection of invasive African sharptooth catfish
- Authors: Kadye, Wilbert T , Booth, Anthony J
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125276 , vital:35767 , http://dx.doi.10.1111/j.1469-7998.2012.00960.x
- Description: Information on the movement behaviour and habitat use by non-native invasive African catfish Clarias gariepinus is crucial in understanding and possibly mitigating its potential impacts. The aim of this study was to examine catfish movement and habitat selection within an invaded impoundment in the Eastern Cape, South Africa. Acoustic telemetry data for 10 tagged catfish were analyzed to identify spatial patterns in home ranges and seasonal changes in habitat associations. Long-distance movements were observed for most catfish from common central release point, whereas short-distance movements defined their home ranges and utilization distributions that were categorized as localized within single or multiple habitats. Habitat selection was non-random with most catfish utilizing the shallow river mouth and upper section of the reservoir that were dominated by a rocky substratum interspersed with submerged trees. These localities were likely to be preferred for spawning and/or feeding. Utilization of these habitats by catfish is likely to be associated with probable impact due to predation and interference competition for feeding and breeding grounds with other species. Although most catfish maintained their home ranges throughout the study, seasonal shifts in habitat use, which was reflected by the utilization of deep and silt-dominated habitats, were also observed for some catfish. Non-random habitat use and homing behaviour within single and multiple habitats by non-native sharptooth catfish suggests that its impact within the invaded habitats may be associated with particular habitats both at broad spatial and temporal scales. Protection of habitats from catfish invasion should be considered as a management option to conserve native biota.
- Full Text:
- Date Issued: 2013
Nanoconjugates of CdTe@ ZnS quantum dots with cobalt tetraamino-phthalocyanine
- Authors: Adegoke, Oluwasesan , Antunes, Edith M , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/242049 , vital:50996 , xlink:href="https://doi.org/10.1016/j.jphotochem.2013.02.010"
- Description: The covalent linking of thiol-capped CdTe@ZnS QDs with cobalt tetraamino-phthalocyanine (CoTAPc) has been successfully carried out. Several techniques such as time-resolved fluorescence measurements, thermal gravimetric analysis,transmissionelectronmicroscopy andspectrophotometric techniques were employed to characterize the nanoconjugates. Covalent binding of the QDs with CoTAPc resulted in the fluorescence quenching of the former. In the presence of varying concentrations of superoxide anion (O2 •−), the fluorescence of the QDs in the conjugate was gradually enhanced and the detection limits obtained were 2.1 and 2.4 nM for the smaller and larger QDs, respectively. Based on the excellent selectivity displayed by the nanoconjugates towards O2 •− over other biologically active species, a potential nanosensor was developed.
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- Date Issued: 2013
Nanostructured β-cyclodextrin-hyperbranched polyethyleneimine (β-CD-HPEI) embedded in polysulfone membrane for the removal of humic acid from water
- Authors: Malinga, S P , Arotiba, O A , Mapolie, S F , Diallo, M S , Mamba, Bhekie B , Krause, Rui W M
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125119 , vital:35732 , https://doi.org/10.1080/01496395.2013.809108
- Description: The synthesis of a new β-cyclodextrin-hyperbranched polyethyleneimine (β-CD-HPEI)/polysulfone (PSf) membranes via interfacial polymerization of trimesoyl chloride and β-CD-HPEI is described in this paper. The membranes were characterized by atomic force microscopy (AFM), high resolution scanning electron microscopy (HR-SEM) and contact-angle measurements. Water permeability and rejection data were obtained using a cross-flow filtration system at 0.69 MPa. The membranes were hydrophilic (25° to 63°), showed high humic acid rejection (>amp;80%), and maintained a constant flux throughout the filtration. The modified membranes were rougher than the pristine PSf membranes but they exhibited better antifouling properties due to the hydrophilic surface which acted as a barrier against humic acid deposition. The modification of PSf with β-CD-HPEI resulted in enhanced hydrophilicity and water permeability while still maintaining high humic acid rejection. Supplemental materials are available for this article. Go to the publisher's online edition of Separation Science & Technology to view the supplemental file.
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- Date Issued: 2013
Navi Pillay: Realising Human Rights for All
- Authors: Naidu, Samantha
- Date: 2013
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/157960 , vital:40134 , ISBN 978-1906413453
- Description: Navi Pillay has become one of the world's leading advocates in the field of human rights. This new biography tells the story of her humble beginnings in an Indian family in apartheid South Africa, her struggle to gain an education, and her eventual success by becoming the first black woman in the nation to set up a law practice. She defended many anti-apartheid activists and fought for the rights of the mostly political prisoners of Robben Island. In 1995 Nelson Mandela nominated Pillay as the first black female judge on South Africa's Supreme Court. This is the story of how she overcame enormous obstacles to achieve her dream of human rights.
- Full Text:
- Date Issued: 2013
Negotiating identity and belonging: perspectives of children living in a disadvantaged community in the Eastern Cape province
- Authors: Ridley, Tamerin Amy
- Date: 2013
- Subjects: Group identity , Self-esteem
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/24813 , vital:63598
- Description: Developing an identity with self-esteem and a sense of self-worth is a child’s fundamental right (Vandenbroek, 2001). To encourage identity formation children need to ask and answer questions such as: ‘Who am I?’, ‘Where do I belong?’ and ‘Is it ok to be who I am?’ A child’s identity is shaped largely by his/her experiences with regards to relationships and belonging within communities and familial structures. However, South Africa faces a host of problems, including poverty, violence, HIV/AIDS, all of which contribute to the breakdown of these familial and community structures. Utilising a participatory action framework, this research aims to provide insight into how children living in a disadvantaged community negotiate identity and belonging. This insight into children’s perceptions of identity and belonging is useful for identifying resources within the community which promote a positive sense of identity and belonging, and also to identify areas where support and intervention are required. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2013
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- Date Issued: 2013
New Frontiers of Librarianship
- Authors: Satgoor, Ujala
- Date: 2013
- Language: English
- Type: Conference paper , text
- Identifier: vital:6976 , http://hdl.handle.net/10962/d1007308
- Description: Paper delivered at the Sabinet Client Conference, 6 September 2013
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- Date Issued: 2013