Development and validation of in-process control test kits for biodiesel production
- Authors: Fibi, Pumza Oscarine
- Date: 2013
- Subjects: Biodiesel fuels -- South Africa , Biomass energy -- South Africa
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:10416 , http://hdl.handle.net/10948/d1012645 , Biodiesel fuels -- South Africa , Biomass energy -- South Africa
- Description: The production of biodiesel from vegetable oils is not a new technology; it has been around since the 1950’s and both the research in terms of the different feedstock that can be used and the production of biodiesel has since been gaining momentum as there needs to be a new, sustainable and domestic alternative to petroleum fuels. These petroleum fuels pose enormous threats to the environment and therefore need to be replaced as they are mostly contributing to climate change and global warming not to mention the frequent price hikes which are crippling the South African economy. Biodiesel production using vegetable oils seems to be and is the future and a law has recently been passed which sanctions the production of biofuel locally.[1] South African fuel producers will instigate obligatory blending of fossil fuel with biofuel as the country moves to encourage investment in its biofuels sector. The production of biodiesel locally and the blending of biodiesel with other petroleum products will reduce the country’s dependence on imported fuel. The already established petrochemical companies like BP, Sasol and Engine are therefore mandated to purchase these biofuels if and when the biofuels meet the required South African National Standard (SANS) 1935 requirements. This is then where the challenge comes as most of these growing biofuel companies cannot afford to purchase testing equipment.The growing companiesthen discover upon completion of the biofuel manufacturing process that their product does not meet the required standard specification. The failure translates to a financial loss as the final product can possibly not be reworked. The aim of the project is then to assist these companies who are manufacturing biofuel, by providing them with in-house biofuel process methods which will allow for early detection, should there be a need to redo a step in the process and not wait until the completion of the production process. These in-house process-testing methods will range from pH determination, titration tests which will determine the soap content and the percentage free fatty acid content, water determination, density and visual testing. It is not cost-effective for these biodiesel manufacturers to send their samples for outsource testing as evidently the results obtained would be out of specification hence the need to provide these biodiesel manufacturers with in-house analytical testing techniques that will aid in monitoring of the biodiesel production.
- Full Text:
- Date Issued: 2013
- Authors: Fibi, Pumza Oscarine
- Date: 2013
- Subjects: Biodiesel fuels -- South Africa , Biomass energy -- South Africa
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:10416 , http://hdl.handle.net/10948/d1012645 , Biodiesel fuels -- South Africa , Biomass energy -- South Africa
- Description: The production of biodiesel from vegetable oils is not a new technology; it has been around since the 1950’s and both the research in terms of the different feedstock that can be used and the production of biodiesel has since been gaining momentum as there needs to be a new, sustainable and domestic alternative to petroleum fuels. These petroleum fuels pose enormous threats to the environment and therefore need to be replaced as they are mostly contributing to climate change and global warming not to mention the frequent price hikes which are crippling the South African economy. Biodiesel production using vegetable oils seems to be and is the future and a law has recently been passed which sanctions the production of biofuel locally.[1] South African fuel producers will instigate obligatory blending of fossil fuel with biofuel as the country moves to encourage investment in its biofuels sector. The production of biodiesel locally and the blending of biodiesel with other petroleum products will reduce the country’s dependence on imported fuel. The already established petrochemical companies like BP, Sasol and Engine are therefore mandated to purchase these biofuels if and when the biofuels meet the required South African National Standard (SANS) 1935 requirements. This is then where the challenge comes as most of these growing biofuel companies cannot afford to purchase testing equipment.The growing companiesthen discover upon completion of the biofuel manufacturing process that their product does not meet the required standard specification. The failure translates to a financial loss as the final product can possibly not be reworked. The aim of the project is then to assist these companies who are manufacturing biofuel, by providing them with in-house biofuel process methods which will allow for early detection, should there be a need to redo a step in the process and not wait until the completion of the production process. These in-house process-testing methods will range from pH determination, titration tests which will determine the soap content and the percentage free fatty acid content, water determination, density and visual testing. It is not cost-effective for these biodiesel manufacturers to send their samples for outsource testing as evidently the results obtained would be out of specification hence the need to provide these biodiesel manufacturers with in-house analytical testing techniques that will aid in monitoring of the biodiesel production.
- Full Text:
- Date Issued: 2013
The effect of in vitro digestion on selected biological activities of Hypoxis sobolifera corms
- Authors: Van Rooyen, Anzel
- Date: 2013
- Subjects: Potatoes -- Therapeutic use , Medicinal plants
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10344 , http://hdl.handle.net/10948/d1020058
- Description: In South Africa part of the cultural and religious beliefs of the African people is the use of traditional remedies to treat diseases. These remedies are obtained from medicinal plants (Steenkamp, 2003). One of the most frequently traded plants in the Eastern Cape is Hypoxis, commonly known as Afrika patat, or African potato. South African traditional healers instruct patients to brew the fresh Hypoxis corm as a tea and then ingest it (Steenkamp, 2006a). This prompted an investigation into the digestive stability of a traditionally prepared Hypoxis extract. The H. sobolifera extracts were digested using a simulated gastric/small intestinal digestion and their biological activity determined. The hot water H. sobolifera extract before digestion only showed cytotoxic activity against cancer cell lines at very high concentrations which are not likely to be achieved under normal ingestion circumstances. In Chang liver cells on the other hand, chronic exposure to the hot water H. sobolifera extract increased glucose uptake in amounts similar to that of metformin. On the negative side, the glucose utilization stimulation was lost due to the simulated digestion process. The significant inhibition of AGEs by hot water H. sobolifera extract (IC50 of 6.3 Ig/ml) is a very encouraging result as treatment in the management of diabetes. This activity was only slightly reduced by the in vitro digestion process. Also observed was enzyme inhibition activity by traditionally prepared H. sobolifera, with ∝-amylase being inhibited (IC50 of approximately 250 Ig/ml) and therefore preventing or limiting starch breakdown. From the DPPH results it was clear that H. sobolifera, even when digested, is a potent anti-oxidant (IC50 of 134.4 Ig/ml when undigested compared to 162.9 when digested with β-glucosidase added to stomach digestive step). HPLC and TLC experiments revealed that rooperol which has previously been thought to be the compound responsible for the anti-oxidant activity in Hypoxis extracts, was absent from the traditional extract of H. sobolifera and therefore cannot be the sole compound exhibiting anti-oxidant activity; other compounds such as phenolics may be contributing. The phenolic and flavonoid content results revealed very highconcentrations of these compounds in the traditionally prepared H. sobolifera extract. These compounds may therefore play major roles in all of the biological activities observed from treatment with Hypoxis spp. The ROS results yielded interesting and promising results. Using standard or traditionally prepared H. sobolifera extracts, activation of differentiated U937 cells with PMA was greatly enhanced by cotreatment with the extracts, while extracts on their own did not cause significant activation. Future studies should investigate this property of the extracts as a promising immune boosterThe HPLC results showed that hypoxoside was undetectable in the hot water traditional extract and the TLC anti-oxidant experiment proved that rooperol is not present in the hot water traditional extract after treatment with β-glucosidase. This indicates that neither one of the Hypoxis compounds previously believed to be responsible for the biological activities observed are present in the extract when prepared the traditional way. Therefore, the biological activities observed in this study can be attributed to other phytochemical compounds.
- Full Text:
- Date Issued: 2013
- Authors: Van Rooyen, Anzel
- Date: 2013
- Subjects: Potatoes -- Therapeutic use , Medicinal plants
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10344 , http://hdl.handle.net/10948/d1020058
- Description: In South Africa part of the cultural and religious beliefs of the African people is the use of traditional remedies to treat diseases. These remedies are obtained from medicinal plants (Steenkamp, 2003). One of the most frequently traded plants in the Eastern Cape is Hypoxis, commonly known as Afrika patat, or African potato. South African traditional healers instruct patients to brew the fresh Hypoxis corm as a tea and then ingest it (Steenkamp, 2006a). This prompted an investigation into the digestive stability of a traditionally prepared Hypoxis extract. The H. sobolifera extracts were digested using a simulated gastric/small intestinal digestion and their biological activity determined. The hot water H. sobolifera extract before digestion only showed cytotoxic activity against cancer cell lines at very high concentrations which are not likely to be achieved under normal ingestion circumstances. In Chang liver cells on the other hand, chronic exposure to the hot water H. sobolifera extract increased glucose uptake in amounts similar to that of metformin. On the negative side, the glucose utilization stimulation was lost due to the simulated digestion process. The significant inhibition of AGEs by hot water H. sobolifera extract (IC50 of 6.3 Ig/ml) is a very encouraging result as treatment in the management of diabetes. This activity was only slightly reduced by the in vitro digestion process. Also observed was enzyme inhibition activity by traditionally prepared H. sobolifera, with ∝-amylase being inhibited (IC50 of approximately 250 Ig/ml) and therefore preventing or limiting starch breakdown. From the DPPH results it was clear that H. sobolifera, even when digested, is a potent anti-oxidant (IC50 of 134.4 Ig/ml when undigested compared to 162.9 when digested with β-glucosidase added to stomach digestive step). HPLC and TLC experiments revealed that rooperol which has previously been thought to be the compound responsible for the anti-oxidant activity in Hypoxis extracts, was absent from the traditional extract of H. sobolifera and therefore cannot be the sole compound exhibiting anti-oxidant activity; other compounds such as phenolics may be contributing. The phenolic and flavonoid content results revealed very highconcentrations of these compounds in the traditionally prepared H. sobolifera extract. These compounds may therefore play major roles in all of the biological activities observed from treatment with Hypoxis spp. The ROS results yielded interesting and promising results. Using standard or traditionally prepared H. sobolifera extracts, activation of differentiated U937 cells with PMA was greatly enhanced by cotreatment with the extracts, while extracts on their own did not cause significant activation. Future studies should investigate this property of the extracts as a promising immune boosterThe HPLC results showed that hypoxoside was undetectable in the hot water traditional extract and the TLC anti-oxidant experiment proved that rooperol is not present in the hot water traditional extract after treatment with β-glucosidase. This indicates that neither one of the Hypoxis compounds previously believed to be responsible for the biological activities observed are present in the extract when prepared the traditional way. Therefore, the biological activities observed in this study can be attributed to other phytochemical compounds.
- Full Text:
- Date Issued: 2013
Assemblage dynamics of larval fishes associated with various shallow water nursery habitats in Algoa Bay, South Africa
- Authors: Pattrick, Paula
- Date: 2013
- Subjects: Spawning -- South Africa -- Algoa Bay , Fishes -- Larvae -- South Africa -- Algoa Bay
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:10727 , http://hdl.handle.net/10948/d1021042
- Description: The success of the larval stage in fishes plays a critical role in structuring adult fish populations. It is well understood that juveniles of many marine fish species are closely associated with nearshore and coastal habitats that serve as nursery areas while adult assemblages are more widely distributed. The larval phase however, particularly pertaining to nursery habitat use, remains poorly understood in South Africa. A mixed-method, larval and juvenile fish study was conducted in the warm-temperate shallow coastal waters of Algoa Bay, South Africa. Two years (2010 – 2012) of seasonal sampling at 27 stations at various habitat types revealed distinct spatio-temporal patterns in larval fish composition and abundance. In total, 164 species from 50 families were collected in the nearshore (<30 m), over reef and sand, in the surf zone and large estuarine habitats in Algoa Bay. Engraulidae dominated the larval fish catch in the nearshore (38.4 percent) and over the selected reef and sand habitats (37.8 percent). Cynoglossidae (28.1 percent) and Sparidae (8.4 percent) were the second and third most abundant fish families in the nearshore. In subtidal reef and sand habitats, Gobiidae (23.4 percent) and Clupeidae (9.2 percent) were the second and third most abundant families respectively. Sparidae dominated (71.4 percent) surf zones followed by Soleidae (10.8 percent) and Mugilidae (5.3 percent), while in the mouth area of two permanently open estuaries in Algoa Bay, Gobiidae (35.9 percent) Sparidae (30.1 percent) and Mugilidae (12.4 percent) dominated. Several species from the Blenniidae, Gobiesocidae, Gobiidae, Scorpaenidae and Tripterygiidae fish families complete their pelagic larval phase in the reef habitats in Algoa Bay. These reef habitats therefore serve as important nursery areas for the larvae of benthic species. The sand and nearshore habitats serve as nursery areas for Clupeidae and Engraulidae. Highest densities of Carangidae, Sciaenidae and Sparidae larvae were observed at the subtidal reef habitats indicating that this area is important for accumulation of the early developmental stages of these fishes. The nearshore serves as an important accumulation habitat for species in the Haemulidae and Soleidae families whose juveniles are known to recruit into estuarine nursery areas. The nearshore therefore is a specific area of presettlement buildup of larvae prior to settlement of larvae or juveniles in estuarine nursery areas. Cynoglossidae larvae are also using the nearshore as an accumulation area prior to settlement and recruitment into adult populations occurring in shallow water habitats. In the surf zone, not only are estuary-dependent fish species utilizing this habitat as a nursery area, but marinespecies whose adults are either associated with rocky shores or surf zones are similarly using these areas as nursery habitats. In addition, estuary-dependent fish species which spawn in the marine environment are actively recruiting into estuarine nursery areas on both tides. Active recruitment against the outgoing ebb tide flow was a conclusive finding in this study. These larvae and juveniles are actively swimming against the ebb tide in the shallower, slower-flowing marginal areas where current flow is reduced. Strong environmental gradients on multiple temporal and spatial scales occur in Algoa Bay. Seasonal patterns were evident in larval densities, richness and diversity. Peaks in density, richness and diversity occurred during spring and summer (September to February) associated with increasing water temperatures and a high productivity providing a good food environment for larval fishes. Thereafter densities declined steadily as a result of natural mortality, settlement or adult spawning activity, until autumn when numbers dropped suddenly as the surviving larvae moved to settlement habitats. The relationship between larval fish and environmental variables provides information useful to determine distributions. Therefore the Bay is particularly suited for modelling larval fish distributions. Using generalized linear models, larval fish density in the nearshore of Algoa Bay responded to different ocean features to varying degrees. Larval fish density responded positively to both upwelling and when warm water plumes, originating from an Agulhas Current meander, entered Algoa Bay. On subtidal reefs, habitat complexity played an important role in determining larval fish composition and diversity. The less structurally complex, reefassociated sand habitats, yielded higher species richness and diversity than the high and low profile reef habitats. Therefore, it is likely that the importance of less structurally complex habitats has been overlooked relative to other habitats in terms of their function for larval fishes. In the surf zone, wave period and wave height, which can be related to exposure, were the most significant environmental factors influencing larval fish assemblages. Greatest species diversity was observed in the surf zone habitats heavily influenced by wave action in the windward sector of Algoa Bay. The identification of spawning areas and the mapping of distributions of early developmental stages of fishes are important in providing data pertaining to the protection of these habitats. This is particularly relevant as a new marine protected area MPA)is planned for the eastern sector of Algoa Bay. Results from retrogressive plots indicate that the spawning locations of several species of coastal and pelagic fishes are occurring outside of the Bay, with the bay therefore serving as an accumulation area. Furthermore, distribution modelling results demonstrate that the early developmental stages of fishes in the shallow coastal habitats of Algoa Bay exhibit high spatial variability in their distributions. Results presented in this thesis help fill the knowledge gaps critical to the understanding of larval fish nursery areas of several economically and ecologically important fish species in Algoa Bay.
- Full Text:
- Date Issued: 2013
- Authors: Pattrick, Paula
- Date: 2013
- Subjects: Spawning -- South Africa -- Algoa Bay , Fishes -- Larvae -- South Africa -- Algoa Bay
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:10727 , http://hdl.handle.net/10948/d1021042
- Description: The success of the larval stage in fishes plays a critical role in structuring adult fish populations. It is well understood that juveniles of many marine fish species are closely associated with nearshore and coastal habitats that serve as nursery areas while adult assemblages are more widely distributed. The larval phase however, particularly pertaining to nursery habitat use, remains poorly understood in South Africa. A mixed-method, larval and juvenile fish study was conducted in the warm-temperate shallow coastal waters of Algoa Bay, South Africa. Two years (2010 – 2012) of seasonal sampling at 27 stations at various habitat types revealed distinct spatio-temporal patterns in larval fish composition and abundance. In total, 164 species from 50 families were collected in the nearshore (<30 m), over reef and sand, in the surf zone and large estuarine habitats in Algoa Bay. Engraulidae dominated the larval fish catch in the nearshore (38.4 percent) and over the selected reef and sand habitats (37.8 percent). Cynoglossidae (28.1 percent) and Sparidae (8.4 percent) were the second and third most abundant fish families in the nearshore. In subtidal reef and sand habitats, Gobiidae (23.4 percent) and Clupeidae (9.2 percent) were the second and third most abundant families respectively. Sparidae dominated (71.4 percent) surf zones followed by Soleidae (10.8 percent) and Mugilidae (5.3 percent), while in the mouth area of two permanently open estuaries in Algoa Bay, Gobiidae (35.9 percent) Sparidae (30.1 percent) and Mugilidae (12.4 percent) dominated. Several species from the Blenniidae, Gobiesocidae, Gobiidae, Scorpaenidae and Tripterygiidae fish families complete their pelagic larval phase in the reef habitats in Algoa Bay. These reef habitats therefore serve as important nursery areas for the larvae of benthic species. The sand and nearshore habitats serve as nursery areas for Clupeidae and Engraulidae. Highest densities of Carangidae, Sciaenidae and Sparidae larvae were observed at the subtidal reef habitats indicating that this area is important for accumulation of the early developmental stages of these fishes. The nearshore serves as an important accumulation habitat for species in the Haemulidae and Soleidae families whose juveniles are known to recruit into estuarine nursery areas. The nearshore therefore is a specific area of presettlement buildup of larvae prior to settlement of larvae or juveniles in estuarine nursery areas. Cynoglossidae larvae are also using the nearshore as an accumulation area prior to settlement and recruitment into adult populations occurring in shallow water habitats. In the surf zone, not only are estuary-dependent fish species utilizing this habitat as a nursery area, but marinespecies whose adults are either associated with rocky shores or surf zones are similarly using these areas as nursery habitats. In addition, estuary-dependent fish species which spawn in the marine environment are actively recruiting into estuarine nursery areas on both tides. Active recruitment against the outgoing ebb tide flow was a conclusive finding in this study. These larvae and juveniles are actively swimming against the ebb tide in the shallower, slower-flowing marginal areas where current flow is reduced. Strong environmental gradients on multiple temporal and spatial scales occur in Algoa Bay. Seasonal patterns were evident in larval densities, richness and diversity. Peaks in density, richness and diversity occurred during spring and summer (September to February) associated with increasing water temperatures and a high productivity providing a good food environment for larval fishes. Thereafter densities declined steadily as a result of natural mortality, settlement or adult spawning activity, until autumn when numbers dropped suddenly as the surviving larvae moved to settlement habitats. The relationship between larval fish and environmental variables provides information useful to determine distributions. Therefore the Bay is particularly suited for modelling larval fish distributions. Using generalized linear models, larval fish density in the nearshore of Algoa Bay responded to different ocean features to varying degrees. Larval fish density responded positively to both upwelling and when warm water plumes, originating from an Agulhas Current meander, entered Algoa Bay. On subtidal reefs, habitat complexity played an important role in determining larval fish composition and diversity. The less structurally complex, reefassociated sand habitats, yielded higher species richness and diversity than the high and low profile reef habitats. Therefore, it is likely that the importance of less structurally complex habitats has been overlooked relative to other habitats in terms of their function for larval fishes. In the surf zone, wave period and wave height, which can be related to exposure, were the most significant environmental factors influencing larval fish assemblages. Greatest species diversity was observed in the surf zone habitats heavily influenced by wave action in the windward sector of Algoa Bay. The identification of spawning areas and the mapping of distributions of early developmental stages of fishes are important in providing data pertaining to the protection of these habitats. This is particularly relevant as a new marine protected area MPA)is planned for the eastern sector of Algoa Bay. Results from retrogressive plots indicate that the spawning locations of several species of coastal and pelagic fishes are occurring outside of the Bay, with the bay therefore serving as an accumulation area. Furthermore, distribution modelling results demonstrate that the early developmental stages of fishes in the shallow coastal habitats of Algoa Bay exhibit high spatial variability in their distributions. Results presented in this thesis help fill the knowledge gaps critical to the understanding of larval fish nursery areas of several economically and ecologically important fish species in Algoa Bay.
- Full Text:
- Date Issued: 2013
Analysis of temporal and spatial variations in water storage by means of gravimetric and hydrologic methods in the region around the South African gravimetric observation station
- Authors: Mahed, Gaathier
- Date: 2013
- Subjects: Ground water -- South Africa , Geology -- South Africa , Hydrogeology -- South Africa , Water-supply -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/6714 , vital:21137
- Description: This work examines the use of gravity data and its application to subsurface water reservoirs in the immediate vicinity of the South African Geodynamic Observatory, Sutherland (SAGOS), situated in a semi-arid region of the Karoo region of South Africa, and underlain by the Karoo sedimentary rocks intruded by dolerite dykes and sills. SAGOS houses the only supergravity metre (SG) in Africa, and this thesis sets out to test its use in monitoring groundwater dynamics using hydrological and gravity data. The main aim of this work is the application of the SG data, in conjunction with hydrological data, to better understand episodic recharge of subsurface reservoirs. The importance of water as a resource, globally and specifically the Karoo, is reviewed in conjunction with supply and demand of water. This is to contextualise the socio-economic, technical as well as policy issues related to water resource management. Applicable technologies for water resource management and efficient water use are highlighted and the application of gravity to hydrology is introduced, including satellite as well as ground based tools. In addition, arid zone hydrology as well as recharge and its mechanisms are analysed in order to better understand these processes when examined from gravity measurements. Issues related to understanding flow within the vadose zone as well as in secondary aquifers are examined, and gravity residuals and subsurface hydrology are highlighted. Thereafter, a conceptual groundwater flow modelof the study area is developed using multiple tools. First, the geology around SAGOS was mapped using SPOT 5 imagery and then ground truthed. Second, stable isotopes and water chemistry analysis was undertaken on water samples from selected boreholes. The results allude to preferential flow acting as the main mechanism for groundwater recharge. Follow-up pump-tests illustrate that fracture connectivity is greatest at close proximity to the dyke. Soil mapping, using aerial photography was also undertaken. Duplex soils, enriched with clay at depth, dominate the study area. Using in-situ infiltration tests, it is shown that the alluvium, which lines the river beds, has a higher hydraulic conductivity than the other soils, confirming that these streams act as preferential conduits for subsurface recharge. Precipitation events were correlated against gravity residuals at 4 wells, over different time periods. The results are examined using time series analyses. Gravity residuals from well SA BK07, over a period of 24 hours after the rainfall event, delineate instances of negative correlations, as well as strong positive correlations (of up to 0.9). On the whole however, correlations between gravity and groundwater at SA BK07 are variable and weak, and in conjunction with water level measurements and water chemistry, the data suggest that this well is located in a dynamic conduit (throughflow) and not in a permanent groundwater reservoir. By contrast, other wells show strong positive correlations between gravity residuals and water levels following episodic recharge events for a later time series. Correlations between the water levels and gravity residuals in wells SA BK04, SA BK05 and SA BK 01 are in excess of 0.7 for specific rainfall events. In summary, the results suggests that gravity is an excellent tool for measuring episodic groundwater recharge within the immediate vicinity of the SAGOS. This implies that gravity can aid in monitoring groundwater losses/gains in arid and semi-arid areas. Recommendations for future work are highlighted at the end; these include the possible use of hydrological modelling of reservoirs at various scales and then comparing these results to the SG as well as GOCE and GRACE satellites data, and then improving numerical modelling of the groundwater dynamics for sites like Sutherland and the surrounding arid Karoo region, where sparse water shortages, and potential pollution related to fracking for shale-gas, are likely to compete with established water needs for farming and human consumption. It is also suggested that the gravity modelling be examined to better understand site specific scenarios and thus aid in improving the processing of the gravity signal.
- Full Text:
- Date Issued: 2013
- Authors: Mahed, Gaathier
- Date: 2013
- Subjects: Ground water -- South Africa , Geology -- South Africa , Hydrogeology -- South Africa , Water-supply -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/6714 , vital:21137
- Description: This work examines the use of gravity data and its application to subsurface water reservoirs in the immediate vicinity of the South African Geodynamic Observatory, Sutherland (SAGOS), situated in a semi-arid region of the Karoo region of South Africa, and underlain by the Karoo sedimentary rocks intruded by dolerite dykes and sills. SAGOS houses the only supergravity metre (SG) in Africa, and this thesis sets out to test its use in monitoring groundwater dynamics using hydrological and gravity data. The main aim of this work is the application of the SG data, in conjunction with hydrological data, to better understand episodic recharge of subsurface reservoirs. The importance of water as a resource, globally and specifically the Karoo, is reviewed in conjunction with supply and demand of water. This is to contextualise the socio-economic, technical as well as policy issues related to water resource management. Applicable technologies for water resource management and efficient water use are highlighted and the application of gravity to hydrology is introduced, including satellite as well as ground based tools. In addition, arid zone hydrology as well as recharge and its mechanisms are analysed in order to better understand these processes when examined from gravity measurements. Issues related to understanding flow within the vadose zone as well as in secondary aquifers are examined, and gravity residuals and subsurface hydrology are highlighted. Thereafter, a conceptual groundwater flow modelof the study area is developed using multiple tools. First, the geology around SAGOS was mapped using SPOT 5 imagery and then ground truthed. Second, stable isotopes and water chemistry analysis was undertaken on water samples from selected boreholes. The results allude to preferential flow acting as the main mechanism for groundwater recharge. Follow-up pump-tests illustrate that fracture connectivity is greatest at close proximity to the dyke. Soil mapping, using aerial photography was also undertaken. Duplex soils, enriched with clay at depth, dominate the study area. Using in-situ infiltration tests, it is shown that the alluvium, which lines the river beds, has a higher hydraulic conductivity than the other soils, confirming that these streams act as preferential conduits for subsurface recharge. Precipitation events were correlated against gravity residuals at 4 wells, over different time periods. The results are examined using time series analyses. Gravity residuals from well SA BK07, over a period of 24 hours after the rainfall event, delineate instances of negative correlations, as well as strong positive correlations (of up to 0.9). On the whole however, correlations between gravity and groundwater at SA BK07 are variable and weak, and in conjunction with water level measurements and water chemistry, the data suggest that this well is located in a dynamic conduit (throughflow) and not in a permanent groundwater reservoir. By contrast, other wells show strong positive correlations between gravity residuals and water levels following episodic recharge events for a later time series. Correlations between the water levels and gravity residuals in wells SA BK04, SA BK05 and SA BK 01 are in excess of 0.7 for specific rainfall events. In summary, the results suggests that gravity is an excellent tool for measuring episodic groundwater recharge within the immediate vicinity of the SAGOS. This implies that gravity can aid in monitoring groundwater losses/gains in arid and semi-arid areas. Recommendations for future work are highlighted at the end; these include the possible use of hydrological modelling of reservoirs at various scales and then comparing these results to the SG as well as GOCE and GRACE satellites data, and then improving numerical modelling of the groundwater dynamics for sites like Sutherland and the surrounding arid Karoo region, where sparse water shortages, and potential pollution related to fracking for shale-gas, are likely to compete with established water needs for farming and human consumption. It is also suggested that the gravity modelling be examined to better understand site specific scenarios and thus aid in improving the processing of the gravity signal.
- Full Text:
- Date Issued: 2013
The fitting of statistical distributions to wind data in coastal areas of South Africa
- Authors: Moodley, Kirshnee
- Date: 2013
- Subjects: Winds -- South Africa -- Port Elizabeth -- Speed -- Measurement , Wind power -- South Africa -- Port Elizabeth , Winds -- South Africa -- Port Elizabeth -- Measurement -- Mathematical models
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10584
- Description: Coastal South African cities like Port Elizabeth are said to have a strong potential for wind energy. This study aims to model wind data in order to be able assess the power potential belonging to a given site. The main challenge in modelling wind direction data is that it is categorized as circular data and therefore requires special techniques for handling that are different from usual statistical samples. Statistical tools such as descriptive measures and distribution fitting, were re-invented for directional data by researchers in this field. The von Mises distribution is a predominant distribution in circular statistics and is commonly used to describe wind directions. In this study, the circular principles described by previous researchers were developed by using the statistical software, Mathematica. Graphical methods to present the wind data were developed to give an overview of the behaviour of the winds in any given area. Data collected at Coega, an area near Port Elizabeth, South Africa, was used to illustrate the models which were established in this study. Circular distributions were fit to the directional data in order to make appropriate conclusions about the prevailing wind directions in this area.
- Full Text:
- Date Issued: 2013
- Authors: Moodley, Kirshnee
- Date: 2013
- Subjects: Winds -- South Africa -- Port Elizabeth -- Speed -- Measurement , Wind power -- South Africa -- Port Elizabeth , Winds -- South Africa -- Port Elizabeth -- Measurement -- Mathematical models
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10584
- Description: Coastal South African cities like Port Elizabeth are said to have a strong potential for wind energy. This study aims to model wind data in order to be able assess the power potential belonging to a given site. The main challenge in modelling wind direction data is that it is categorized as circular data and therefore requires special techniques for handling that are different from usual statistical samples. Statistical tools such as descriptive measures and distribution fitting, were re-invented for directional data by researchers in this field. The von Mises distribution is a predominant distribution in circular statistics and is commonly used to describe wind directions. In this study, the circular principles described by previous researchers were developed by using the statistical software, Mathematica. Graphical methods to present the wind data were developed to give an overview of the behaviour of the winds in any given area. Data collected at Coega, an area near Port Elizabeth, South Africa, was used to illustrate the models which were established in this study. Circular distributions were fit to the directional data in order to make appropriate conclusions about the prevailing wind directions in this area.
- Full Text:
- Date Issued: 2013
The diet of the Subantarctic (A. Tropicalis) and Antarctic (A. Gazella) Fur seal at Marion Island
- Mgibantaka, Nonkoliso Felicia
- Authors: Mgibantaka, Nonkoliso Felicia
- Date: 2013
- Subjects: Southern fur seals -- Feeding and feeds -- Antarctica , Seals (Animals) -- Antarctica , Marine ecology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10729 , http://hdl.handle.net/10948/d1021104
- Description: Studying the diet of marine top predators is important for understanding the trophic ecology of the predators but also the distribution, abundance and seasonal variation of prey species. Dietary studies on subantarctic fur seals (Arctocephalus tropicalis) and Antarctic fur seals (A. gazella) is important for assessing their role as predators in the Southern Ocean. Seven hundred and sixty four scats were collected from an A. tropicalis colony at Cape Davis while 659 scats were collected from an A. gazella colony at Watertunnel stream from April 2006 to March 2010. Hard remains such as fish otoliths and cephalopod beaks were used for identification and quantification of prey. A. tropicalis diet comprised of three animal groups (fish, cephalopod and crustaceans) while A. gazella diet was made up of four animal groups (fish, cephalopods, crustaceans and seabirds). Fish contributed substantially more than other prey items in the diet of both fur seal species over the study period. Both species fed predominantly on fish of the family Myctophidae which constituted a numerical abundance of 98.9 percent in scats for both species combined. A. tropicalis fed on fish prey species from eight families, namely: Myctophidae, Notosudidae, Paralepididae, Nototheniidae, Bathylagidae, Chlorophthalmidae, Sternoptychidae and Photichthyidae while A. gazella fed on fish from six of these families, with Chlorophthalmidae and Photichthyidae not represented in its diet. Cephalopods and crustaceans were found in low numbers in the diet of both seal species and seabird remains were only found in the diet of A. gazella. Numerically, the most dominant species were from the genus Gymnoscopelus followed by Protomyctophum and then Electrona. In the diet of A. tropicalis, Gymnoscopelus contributed 66 percent, Protomyctophum 18 percent and Electrona 5.9 percent while in the diet of A. gazella Gymnoscopelus, Protomyctophum and Electrona contributed 59.43 percent, 20.24 percent and 14.24 percent respectively. Gymnoscopelus species comprised G. piabilis, G. bolini, G. braueri, G. fraseri, and G. nicholsi; Protomyctophum comprised Protomyctophum bolini, P. choriodon and P. tenisoni while Electrona comprised Electrona antarctica, E. carlsbergi and E. subaspera. Gymnoscopelus piabilis was the most dominant species from this genus while Electrona carlsbergi and Protomyctophum tenisoni dominated the genus Electrona and Protomyctophum respectively. The total number of fish species utilised by A. tropicalis was twenty four while twenty three fish species were identified in A. gazella diet. Bathysauropsis gracilis only featured in the diet of A. tropicalis while Lampanyctus ater and Lepidonotothen larseni were only found in the diet of A. gazella. To compare the diet of the seal species between seasons, a year was divided into three seasons, early summer, late summer and winter. Their diet was also compared between years from 2006 to 2010. Myctophid fish species showed substantial seasonal and annual fluctuations in the diet of the two fur seal species. E. carlsbergi, G. nicholsi and G. piabilis increased in numerical abundance in winter while Melectrona ventralis increased in late summer. The numerical abundance of G. fraseri and P. tenisoni increased in early summer. Generally, the two fur seal species fed on the same prey species. However, there was a significant difference when the diet of both fur seals was compared seasonally. There was no significant difference when the diet of A. gazella was compared annually, although such a difference was apparent for A. tropicalis.
- Full Text:
- Date Issued: 2013
- Authors: Mgibantaka, Nonkoliso Felicia
- Date: 2013
- Subjects: Southern fur seals -- Feeding and feeds -- Antarctica , Seals (Animals) -- Antarctica , Marine ecology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10729 , http://hdl.handle.net/10948/d1021104
- Description: Studying the diet of marine top predators is important for understanding the trophic ecology of the predators but also the distribution, abundance and seasonal variation of prey species. Dietary studies on subantarctic fur seals (Arctocephalus tropicalis) and Antarctic fur seals (A. gazella) is important for assessing their role as predators in the Southern Ocean. Seven hundred and sixty four scats were collected from an A. tropicalis colony at Cape Davis while 659 scats were collected from an A. gazella colony at Watertunnel stream from April 2006 to March 2010. Hard remains such as fish otoliths and cephalopod beaks were used for identification and quantification of prey. A. tropicalis diet comprised of three animal groups (fish, cephalopod and crustaceans) while A. gazella diet was made up of four animal groups (fish, cephalopods, crustaceans and seabirds). Fish contributed substantially more than other prey items in the diet of both fur seal species over the study period. Both species fed predominantly on fish of the family Myctophidae which constituted a numerical abundance of 98.9 percent in scats for both species combined. A. tropicalis fed on fish prey species from eight families, namely: Myctophidae, Notosudidae, Paralepididae, Nototheniidae, Bathylagidae, Chlorophthalmidae, Sternoptychidae and Photichthyidae while A. gazella fed on fish from six of these families, with Chlorophthalmidae and Photichthyidae not represented in its diet. Cephalopods and crustaceans were found in low numbers in the diet of both seal species and seabird remains were only found in the diet of A. gazella. Numerically, the most dominant species were from the genus Gymnoscopelus followed by Protomyctophum and then Electrona. In the diet of A. tropicalis, Gymnoscopelus contributed 66 percent, Protomyctophum 18 percent and Electrona 5.9 percent while in the diet of A. gazella Gymnoscopelus, Protomyctophum and Electrona contributed 59.43 percent, 20.24 percent and 14.24 percent respectively. Gymnoscopelus species comprised G. piabilis, G. bolini, G. braueri, G. fraseri, and G. nicholsi; Protomyctophum comprised Protomyctophum bolini, P. choriodon and P. tenisoni while Electrona comprised Electrona antarctica, E. carlsbergi and E. subaspera. Gymnoscopelus piabilis was the most dominant species from this genus while Electrona carlsbergi and Protomyctophum tenisoni dominated the genus Electrona and Protomyctophum respectively. The total number of fish species utilised by A. tropicalis was twenty four while twenty three fish species were identified in A. gazella diet. Bathysauropsis gracilis only featured in the diet of A. tropicalis while Lampanyctus ater and Lepidonotothen larseni were only found in the diet of A. gazella. To compare the diet of the seal species between seasons, a year was divided into three seasons, early summer, late summer and winter. Their diet was also compared between years from 2006 to 2010. Myctophid fish species showed substantial seasonal and annual fluctuations in the diet of the two fur seal species. E. carlsbergi, G. nicholsi and G. piabilis increased in numerical abundance in winter while Melectrona ventralis increased in late summer. The numerical abundance of G. fraseri and P. tenisoni increased in early summer. Generally, the two fur seal species fed on the same prey species. However, there was a significant difference when the diet of both fur seals was compared seasonally. There was no significant difference when the diet of A. gazella was compared annually, although such a difference was apparent for A. tropicalis.
- Full Text:
- Date Issued: 2013
The medicinal plant Sutherlandia Frutescens regulates gene expression to reverse insulin resistace in rats
- Authors: Fortuin, Melissa
- Date: 2013
- Subjects: Insulin resistance , Medicinal plants , Genetic regulation , Insulin resistance -- Animal models
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10349 , http://hdl.handle.net/10948/d1020823
- Description: Obesity can lead to Type 2 Diabetes, both conditions increase in association with physical inactivity and high-energy diets, resulting in elevated blood glucose, decreased insulin sensitivity and increased insulin resistance. Sutherlandia frutescens (S.frutescens), an anti-diabetic plant, reverses and prevents insulin resistance in a rat model and human cell culture model. Gene expression analysis in hepatocyte cultures, identified genes down regulated in insulin resistance and up regulated by S.frutescens. These included genes encoding vesicle transporter proteins, hypothesised to be linked to hepatic lipid accumulation and lipid droplet formation during insulin resistance. The aim of this study was to investigate critical genes involved in lipid droplet formation, vesicle assembly and transport in high fat diet (HFD)-induced insulin resistant rat liver tissue during the development of insulin resistance and the reversal of these changes by S.frutescens. Rats were fed a low fat diet (LFD) or HFD supplemented with S.frutescens for 2, 4 and 8 weeks. Rats fed a HFD for 12 weeks developed insulin resistance, confirmed by plasma glucose and insulin levels (compared to normal controls). Groups of these rats were gavaged with S. frutescens (50mg/kg BW), Metformin (13mg/kg BW) or water for a further 4 weeks and starved for 12 hours, anaesthetized and blood removed by heart puncture. Liver was stored in RNA-Later™ for qRT-PCR and snap-frozen in liquid nitrogen for western blotting and confocal microscopy analysis. Changes in expression of vesicle transporter genes VAMP3 and NSF were analysed by qRT-PCR and changes in the protein expression by western blotting analysis. Proteins were localised within the liver by confocal immunohistochemistry using ZEN lite™ software. Statistical analysis was performed using One-Way ANOVA and unpaired t-test. mRNA gene expression of vesicle transport components VAMP3, NSF and SNAP25 showed relatively moderate changes with considerable individual variation within control or experimental groups. Uncorrelated changes in mRNA and protein products were found and may be due to differential regulation by siRNA. Proteins also showed altered staining patterns in high fat diet rats that reverted towards normal on S. frutescens treatment, potentially reflecting functional changes associated with transport of lipid-filled vesicles.
- Full Text:
- Date Issued: 2013
- Authors: Fortuin, Melissa
- Date: 2013
- Subjects: Insulin resistance , Medicinal plants , Genetic regulation , Insulin resistance -- Animal models
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10349 , http://hdl.handle.net/10948/d1020823
- Description: Obesity can lead to Type 2 Diabetes, both conditions increase in association with physical inactivity and high-energy diets, resulting in elevated blood glucose, decreased insulin sensitivity and increased insulin resistance. Sutherlandia frutescens (S.frutescens), an anti-diabetic plant, reverses and prevents insulin resistance in a rat model and human cell culture model. Gene expression analysis in hepatocyte cultures, identified genes down regulated in insulin resistance and up regulated by S.frutescens. These included genes encoding vesicle transporter proteins, hypothesised to be linked to hepatic lipid accumulation and lipid droplet formation during insulin resistance. The aim of this study was to investigate critical genes involved in lipid droplet formation, vesicle assembly and transport in high fat diet (HFD)-induced insulin resistant rat liver tissue during the development of insulin resistance and the reversal of these changes by S.frutescens. Rats were fed a low fat diet (LFD) or HFD supplemented with S.frutescens for 2, 4 and 8 weeks. Rats fed a HFD for 12 weeks developed insulin resistance, confirmed by plasma glucose and insulin levels (compared to normal controls). Groups of these rats were gavaged with S. frutescens (50mg/kg BW), Metformin (13mg/kg BW) or water for a further 4 weeks and starved for 12 hours, anaesthetized and blood removed by heart puncture. Liver was stored in RNA-Later™ for qRT-PCR and snap-frozen in liquid nitrogen for western blotting and confocal microscopy analysis. Changes in expression of vesicle transporter genes VAMP3 and NSF were analysed by qRT-PCR and changes in the protein expression by western blotting analysis. Proteins were localised within the liver by confocal immunohistochemistry using ZEN lite™ software. Statistical analysis was performed using One-Way ANOVA and unpaired t-test. mRNA gene expression of vesicle transport components VAMP3, NSF and SNAP25 showed relatively moderate changes with considerable individual variation within control or experimental groups. Uncorrelated changes in mRNA and protein products were found and may be due to differential regulation by siRNA. Proteins also showed altered staining patterns in high fat diet rats that reverted towards normal on S. frutescens treatment, potentially reflecting functional changes associated with transport of lipid-filled vesicles.
- Full Text:
- Date Issued: 2013
Isocyanide complexes of rhenium
- Authors: Schoultz, Xandri
- Date: 2013
- Subjects: Isocyanides , Rhenium , Complex compounds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10360 , http://hdl.handle.net/10948/d1021069
- Description: This study investigates the synthesis of rhenium(III)-isocyanide complexes with potentially bidentate ligands, as well as the reactivity of isocyanide ligands toward rhenium(I) and (V). The crystal structures of all the complexes have been determined by X-ray diffraction methods. The coordination behaviour of trans-[ReIIICl3(t-BuNC)(PPh3)2] with aniline and its derivatives were investigated. The isocyanide-containing rhenium(V) complexes [ReCl3(t-BuNC)(L)(PPh3)] were isolated, with the ligands H2L (aniline, o-phenylenediamine and anthranilic acid). In all these complexes the dianionic ligands L are coordinated monodentately as the imide. However, with 2-aminophenol the complexes [ReVCl2(t-BuNC)(L)(PPh3)2](ReO4) and [ReIIICl2(t-BuNC)(ibq)(PPh3)2] (ibq- = 2-iminobenzoquinonate) were identified as the products. [ReCl2(t-BuNC)(L)(PPh3)2](ReO4) is the product of a disproportionation reaction from Re(III) to Re(VII) and Re(V). All the above complexes show a distorted octahedral geometry around the rhenium. The products of the reaction of the Re(I) complex [Re(CO)5Cl] with isocyanides (tert-butyl- and cyclohexylisocyanide) are reported. Rhenium(I) tricarbonyl complexes of the form [Re(CO)3(CNR)2Cl] were isolated and they were characterized structurally and spectroscopically. The tricarbonyls are coordinated in the typical facial-fashion, whereas the isocyanides are coordinated cis to each other. The reaction of [Re(CO)3(t-BuNC)2Cl] with H2O led to the formation of the rhenium(I) complex [Re(CO)3(t-BuNC)2(OH2)] in which the aquo ligand can readily be substituted by a more complex ligand. The reaction of the rhenium(V) complexes cis-[ReO2I(PPh3)2] and mer-[ReOCl3(SMe2)(OPPh3)] with isocyanides were studied. The seven-coordinate trigonal prismatic, square faced monocapped rhenium(III) complex [ReI3(t-BuNC)3(PPh3)] was surprisingly isolated upon reacting cis-[ReO2I(PPh3)2] with tert-butyl isocyanide. The dimeric complex (μ-O)[ReOCl2(CNC6H11)2]2 was obtained from the reaction of mer-[ReOCl3(SMe2)(OPPh3)] with cyclohexyl isocyanide.
- Full Text:
- Date Issued: 2013
- Authors: Schoultz, Xandri
- Date: 2013
- Subjects: Isocyanides , Rhenium , Complex compounds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10360 , http://hdl.handle.net/10948/d1021069
- Description: This study investigates the synthesis of rhenium(III)-isocyanide complexes with potentially bidentate ligands, as well as the reactivity of isocyanide ligands toward rhenium(I) and (V). The crystal structures of all the complexes have been determined by X-ray diffraction methods. The coordination behaviour of trans-[ReIIICl3(t-BuNC)(PPh3)2] with aniline and its derivatives were investigated. The isocyanide-containing rhenium(V) complexes [ReCl3(t-BuNC)(L)(PPh3)] were isolated, with the ligands H2L (aniline, o-phenylenediamine and anthranilic acid). In all these complexes the dianionic ligands L are coordinated monodentately as the imide. However, with 2-aminophenol the complexes [ReVCl2(t-BuNC)(L)(PPh3)2](ReO4) and [ReIIICl2(t-BuNC)(ibq)(PPh3)2] (ibq- = 2-iminobenzoquinonate) were identified as the products. [ReCl2(t-BuNC)(L)(PPh3)2](ReO4) is the product of a disproportionation reaction from Re(III) to Re(VII) and Re(V). All the above complexes show a distorted octahedral geometry around the rhenium. The products of the reaction of the Re(I) complex [Re(CO)5Cl] with isocyanides (tert-butyl- and cyclohexylisocyanide) are reported. Rhenium(I) tricarbonyl complexes of the form [Re(CO)3(CNR)2Cl] were isolated and they were characterized structurally and spectroscopically. The tricarbonyls are coordinated in the typical facial-fashion, whereas the isocyanides are coordinated cis to each other. The reaction of [Re(CO)3(t-BuNC)2Cl] with H2O led to the formation of the rhenium(I) complex [Re(CO)3(t-BuNC)2(OH2)] in which the aquo ligand can readily be substituted by a more complex ligand. The reaction of the rhenium(V) complexes cis-[ReO2I(PPh3)2] and mer-[ReOCl3(SMe2)(OPPh3)] with isocyanides were studied. The seven-coordinate trigonal prismatic, square faced monocapped rhenium(III) complex [ReI3(t-BuNC)3(PPh3)] was surprisingly isolated upon reacting cis-[ReO2I(PPh3)2] with tert-butyl isocyanide. The dimeric complex (μ-O)[ReOCl2(CNC6H11)2]2 was obtained from the reaction of mer-[ReOCl3(SMe2)(OPPh3)] with cyclohexyl isocyanide.
- Full Text:
- Date Issued: 2013
A contribution to the theory of prime modules
- Authors: Ssevviiri, David
- Date: 2013
- Subjects: Modules (Algebra) , Radical theory , Rings (Algebra)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10510 , http://hdl.handle.net/10948/d1019923
- Description: This thesis is aimed at generalizing notions of rings to modules. In par-ticular, notions of completely prime ideals, s-prime ideals, 2-primal rings and nilpotency of elements of rings are respectively generalized to completely prime submodules and classical completely prime submodules, s-prime submodules, 2-primal modules and nilpotency of elements of modules. Properties and rad-icals that arise from each of these notions are studied.
- Full Text:
- Date Issued: 2013
- Authors: Ssevviiri, David
- Date: 2013
- Subjects: Modules (Algebra) , Radical theory , Rings (Algebra)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10510 , http://hdl.handle.net/10948/d1019923
- Description: This thesis is aimed at generalizing notions of rings to modules. In par-ticular, notions of completely prime ideals, s-prime ideals, 2-primal rings and nilpotency of elements of rings are respectively generalized to completely prime submodules and classical completely prime submodules, s-prime submodules, 2-primal modules and nilpotency of elements of modules. Properties and rad-icals that arise from each of these notions are studied.
- Full Text:
- Date Issued: 2013
Propagating some commonly-used South African medicinal plants with compost and vermitea
- Authors: Faulconbridge, Steven Craig
- Date: 2013
- Subjects: Medicinal plants -- Propagation -- South Africa , Compost -- South Africa
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:10736 , http://hdl.handle.net/10948/d1020316
- Description: The use of many of South Africa’s medicinal plants has shown marked increase with over 27 million users in South Africa alone. Most plants are still being unsustainably wild-harvested, a major concern for biodiversity conservation. Commercial interest in certain more commonly-used species has increased, with potential to cultivate medicinal plants on a more sustainable basis. Focus has shifted from conventional use of synthetic fertilisers, pesticides and fungicides to more organic methods of plant propagation. Aqueous extract derived from earthworm composted food waste (vermitea) was used to study the germination and rooting success of selected species. Also survival and growth performance of selected plants grown in a medium amended with commercial NPK fertiliser was compared to those grown in the same medium amended with compost and to those grown in the same medium amended with compost with weekly applications of vermitea. No change in germination success was noted. Vermitea showed promising results on the rooting of cuttings. The application of NPK improved growth performance (biomass) significantly for all species tested. However, they had lower root:shoot ratios as well as lower survival rates compared to plants under the compost and compost/vermitea treatments. The improved survival of these plants highlights the potential of these organic treatments on the propagation of selected medicinal plants.
- Full Text:
- Date Issued: 2013
- Authors: Faulconbridge, Steven Craig
- Date: 2013
- Subjects: Medicinal plants -- Propagation -- South Africa , Compost -- South Africa
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:10736 , http://hdl.handle.net/10948/d1020316
- Description: The use of many of South Africa’s medicinal plants has shown marked increase with over 27 million users in South Africa alone. Most plants are still being unsustainably wild-harvested, a major concern for biodiversity conservation. Commercial interest in certain more commonly-used species has increased, with potential to cultivate medicinal plants on a more sustainable basis. Focus has shifted from conventional use of synthetic fertilisers, pesticides and fungicides to more organic methods of plant propagation. Aqueous extract derived from earthworm composted food waste (vermitea) was used to study the germination and rooting success of selected species. Also survival and growth performance of selected plants grown in a medium amended with commercial NPK fertiliser was compared to those grown in the same medium amended with compost and to those grown in the same medium amended with compost with weekly applications of vermitea. No change in germination success was noted. Vermitea showed promising results on the rooting of cuttings. The application of NPK improved growth performance (biomass) significantly for all species tested. However, they had lower root:shoot ratios as well as lower survival rates compared to plants under the compost and compost/vermitea treatments. The improved survival of these plants highlights the potential of these organic treatments on the propagation of selected medicinal plants.
- Full Text:
- Date Issued: 2013
Reactions towards the synthesis of the uncommon P57 cymarose moiety
- Authors: Mahanjana, Lungelwa
- Date: 2013
- Subjects: Chemistry, Organic , Organic compounds -- Synthesis
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: http://hdl.handle.net/10948/6711 , vital:21136
- Description: The work described in this study aims to investigate methods that will improve a lengthy synthetic pathway in the synthesis of the P57 cymarose moiety, and to examine the conformational structure of certain glycosides in order to shed light on the problematic stereochemical issues surrounding the formation of the cymarose glycosyl donor. The cymarose moiety forms part of the trisaccharide derivative present in P57, an appetite suppressant molecule. Modification of reaction steps in the conversion of the stereochemistry at C-3 of a previously reported synthesis of the P57 cymarose moiety was carried out. The first step was the selective oxidation of D-glucal using Pd/C in the presence of acetonitrile. These reaction conditions are more appropriate for the oxidation step to avoid decomposition of the formed molecules. Successive protection of the free OH groups was followed by NaBH4 reduction under stereo-controlled conditions, influenced by CeCl3•7H2O. However, the reduced product could not be isolated from the starting material and this led to ambiguous results when attempting to confirm whether the conversion of the stereochemistry at C-3 had occurred or not. The effect of reaction conditions, such as change in reaction temperature, during the preparation of the cymarose glycosyl donor was studied in order to find suitable reaction conditions to produce α,β-allo derivatives with high stereoselectivity. Compared to the reported synthetic method, this set-up gave improved yields with, unfortunately, similar or slightly lower selectivity to the formation of α-altro:α,β-allo derivative. Examination of the conformational structure of the allal derivative, in order to understand the mechanism at work during the placement of the directing group at C-2, was carried out using molecular modelling. The mechanistic implications of this very short study are discussed and it provides some insights into the likely pathway of the iodination reaction and its selectivity in particular, to the D-allose system.
- Full Text:
- Date Issued: 2013
- Authors: Mahanjana, Lungelwa
- Date: 2013
- Subjects: Chemistry, Organic , Organic compounds -- Synthesis
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: http://hdl.handle.net/10948/6711 , vital:21136
- Description: The work described in this study aims to investigate methods that will improve a lengthy synthetic pathway in the synthesis of the P57 cymarose moiety, and to examine the conformational structure of certain glycosides in order to shed light on the problematic stereochemical issues surrounding the formation of the cymarose glycosyl donor. The cymarose moiety forms part of the trisaccharide derivative present in P57, an appetite suppressant molecule. Modification of reaction steps in the conversion of the stereochemistry at C-3 of a previously reported synthesis of the P57 cymarose moiety was carried out. The first step was the selective oxidation of D-glucal using Pd/C in the presence of acetonitrile. These reaction conditions are more appropriate for the oxidation step to avoid decomposition of the formed molecules. Successive protection of the free OH groups was followed by NaBH4 reduction under stereo-controlled conditions, influenced by CeCl3•7H2O. However, the reduced product could not be isolated from the starting material and this led to ambiguous results when attempting to confirm whether the conversion of the stereochemistry at C-3 had occurred or not. The effect of reaction conditions, such as change in reaction temperature, during the preparation of the cymarose glycosyl donor was studied in order to find suitable reaction conditions to produce α,β-allo derivatives with high stereoselectivity. Compared to the reported synthetic method, this set-up gave improved yields with, unfortunately, similar or slightly lower selectivity to the formation of α-altro:α,β-allo derivative. Examination of the conformational structure of the allal derivative, in order to understand the mechanism at work during the placement of the directing group at C-2, was carried out using molecular modelling. The mechanistic implications of this very short study are discussed and it provides some insights into the likely pathway of the iodination reaction and its selectivity in particular, to the D-allose system.
- Full Text:
- Date Issued: 2013
Technology transfer of antibacterial oral solid dosage form in a pharmaceutical industry
- Authors: Ramloll, Jason
- Date: 2013
- Subjects: Clinical pharmacology Pharmacy -- Research , Pharmaceutical industry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/47913 , vital:40398
- Description: Technology transfer (TT) looks at the transfer of intellectual property from one manufacturing site to another. If carried out successfully, a TT can increase the leveraging capability of the company acquiring the intellectual property together with its brand strength. This in turn aids in a decreased time to market of the final dosage form produced. The aim of the research was to assess the TT of an antibacterial oral solid dosage form from one manufacturing site to another. An expected change of manufacturing equipment from a single-pot to a multi-phase system took place due to the equipment available at the receiving unit. The assessment done by the researcher was performed in the capacity of an observer. The assessment of the TT was performed by observing the critical stages during the transfer process, namely the development and validation stages. Prior to the start of the development stage, a characterisation of innovator was performed. At this stage, innovator batches received from the Sending Unit (SU) were characterised to allow for a means of comparison. The development stage allows for the determination of the robustness of the formulation to be used whilst the validation stage will look at the reproducibility of the chosen formulation. During the development stage, small scale trials were manufactured with amendments made to the original formulation. The amendments allowed for a formulation more comparable to the innovator product with regards to its dissolution profile. The comparable batch produced at a small scale was then up-scaled to a production scale batch and these were compared to the innovator products. The amendments made were on the amount of granulation fluid and binder as well as the method of addition of the disintegrant. When a large scale batch comparable to that of the innovator product was produced, the validation stage was started. With the aim of showing reproducibility of the Receiving Unit (RU) at producing large scale batches, three batches were produced. These were tested for all critical parameters, namely through the loss on drying, particle size distribution, hardness, disintegration and dissolution tests and, the results of the tests were compared to the specifications set by the SU. They were also compared to each other, enabling statistical determination of any significant difference across the batches through the use of comparative statistical analyses. Comparison of batches, at the development and validation stage, was done using the dissolution potential of the batches. These were performed through the use of statistical analyses. The RU made use of the similarity factor (F2) to compare the dissolution profiles. For the purpose of this research, the 1-Way analysis of variance was also used, which compared to the similarity factor, took in consideration the level of variance of the batches produced. Results and discussion: During the development stage amendments were made to produce a batch comparable to the innovator product. The amendments that led to the production of a comparable batch consisted of an extra 14 % of granulation fluid as well as 50 % of the disintegrant being added intra-granularly with the other 50 % added extra-granularly. The amendments were added on top of the amount suggested by the SU. This formulation was used and three batches produced consecutively. On comparison to the specifications set by the SU, the three batches were seen to be within the specifications. On comparison to each other, the dissolution profiles were seen to be different. Conclusion and recommendation: The development stage producing batches within the specifications set by the SU but also comparable with regards to its dissolution profile, was seen to be successful. The validation stage was seen to be unsuccessful. Even though the results of the critical parameters were within specifications, when compared to each other, the batches were seen to be significantly different. This led to the research looking at the TT as a whole as being unsuccessful. The researcher then determined what stage could be improved and how this could be done. The various stages seen as having associated problems were determined and ways to mitigate those were determined. A guideline in the form of a flow chart was proposed to aid a manufacturing facility undertaking a TT to shorten the period of time to completion as well as increase the chance of success.
- Full Text:
- Date Issued: 2013
- Authors: Ramloll, Jason
- Date: 2013
- Subjects: Clinical pharmacology Pharmacy -- Research , Pharmaceutical industry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/47913 , vital:40398
- Description: Technology transfer (TT) looks at the transfer of intellectual property from one manufacturing site to another. If carried out successfully, a TT can increase the leveraging capability of the company acquiring the intellectual property together with its brand strength. This in turn aids in a decreased time to market of the final dosage form produced. The aim of the research was to assess the TT of an antibacterial oral solid dosage form from one manufacturing site to another. An expected change of manufacturing equipment from a single-pot to a multi-phase system took place due to the equipment available at the receiving unit. The assessment done by the researcher was performed in the capacity of an observer. The assessment of the TT was performed by observing the critical stages during the transfer process, namely the development and validation stages. Prior to the start of the development stage, a characterisation of innovator was performed. At this stage, innovator batches received from the Sending Unit (SU) were characterised to allow for a means of comparison. The development stage allows for the determination of the robustness of the formulation to be used whilst the validation stage will look at the reproducibility of the chosen formulation. During the development stage, small scale trials were manufactured with amendments made to the original formulation. The amendments allowed for a formulation more comparable to the innovator product with regards to its dissolution profile. The comparable batch produced at a small scale was then up-scaled to a production scale batch and these were compared to the innovator products. The amendments made were on the amount of granulation fluid and binder as well as the method of addition of the disintegrant. When a large scale batch comparable to that of the innovator product was produced, the validation stage was started. With the aim of showing reproducibility of the Receiving Unit (RU) at producing large scale batches, three batches were produced. These were tested for all critical parameters, namely through the loss on drying, particle size distribution, hardness, disintegration and dissolution tests and, the results of the tests were compared to the specifications set by the SU. They were also compared to each other, enabling statistical determination of any significant difference across the batches through the use of comparative statistical analyses. Comparison of batches, at the development and validation stage, was done using the dissolution potential of the batches. These were performed through the use of statistical analyses. The RU made use of the similarity factor (F2) to compare the dissolution profiles. For the purpose of this research, the 1-Way analysis of variance was also used, which compared to the similarity factor, took in consideration the level of variance of the batches produced. Results and discussion: During the development stage amendments were made to produce a batch comparable to the innovator product. The amendments that led to the production of a comparable batch consisted of an extra 14 % of granulation fluid as well as 50 % of the disintegrant being added intra-granularly with the other 50 % added extra-granularly. The amendments were added on top of the amount suggested by the SU. This formulation was used and three batches produced consecutively. On comparison to the specifications set by the SU, the three batches were seen to be within the specifications. On comparison to each other, the dissolution profiles were seen to be different. Conclusion and recommendation: The development stage producing batches within the specifications set by the SU but also comparable with regards to its dissolution profile, was seen to be successful. The validation stage was seen to be unsuccessful. Even though the results of the critical parameters were within specifications, when compared to each other, the batches were seen to be significantly different. This led to the research looking at the TT as a whole as being unsuccessful. The researcher then determined what stage could be improved and how this could be done. The various stages seen as having associated problems were determined and ways to mitigate those were determined. A guideline in the form of a flow chart was proposed to aid a manufacturing facility undertaking a TT to shorten the period of time to completion as well as increase the chance of success.
- Full Text:
- Date Issued: 2013
An investigation of short-chain fatty acid profiles and influential gastrointenstinal microbiota associated with irritable bowel syndrome
- Authors: Theunissen, Reza
- Date: 2013
- Subjects: Fatty acids in human nutrition , Gastrointestinal system
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10352 , http://hdl.handle.net/10948/d1020943
- Description: Microbiota are present in large numbers and as a diverse population within the gastrointestinal tract. There are approximately 400 different species of microbiota which may be beneficial, harmful or both, but each play an important role in the regulation and modulation of the hosts’ bowel processes (McOrist et al. 2008; Dethlefsen et al. 2008). Many of these colon microbiota allow for saccharolytic fermentation of non-digestible dietary fibres and carbohydrates into by-products and intermediates, followed by a subsequent conversion into short chain fatty acids (SCFAs) (mainly n-butyric acid, propionic acid and acetic acid) each of which play an important role in maintaining colon homeostasis (Topping & Clifton 2001). A balance of ‘good’ microbiota (e.g., Bacteroides spp./ Bifidobacteria spp.) and ‘bad’ microbiota (e.g., Veilonellae) and the optimal production of various SCFAs within the gut could possibly allow for proper functioning of the large intestine and assist in decreasing the onset of various colonic disorders such as Irritable Bowel Syndrome (IBS). The sample group for the study consists of male and female patients, with an average age of 40 to 50 years old, whom of which have been diagnosed with either constipation IBS (C-IBS) or diarrhoea IBS (D-IBS) via the Rome III criteria system for IBS diagnosis. DNA and SCFA extractions were optimised for human stool, colonic fluid and tissue biopsy sample obtained from the aforementioned patients. Optimization steps allowed for starting material with high analysis integrity. Different methods of microbiota analysis, such as ARISA, were investigated; however, real-time qPCR was selected as the best method to identify and quantify specific microbiota. Extracted SCFAs were separated via gas chromatography and identified and quantified via Mass Spectrometry. Significant changes in microbial content and SCFA profiles were found to be associated with healthy and IBS patients. Results obtained would however be influenced by external factors typical of clinical studies of this nature. This study allows for opportunities for future research into understanding IBS.
- Full Text:
- Date Issued: 2013
- Authors: Theunissen, Reza
- Date: 2013
- Subjects: Fatty acids in human nutrition , Gastrointestinal system
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10352 , http://hdl.handle.net/10948/d1020943
- Description: Microbiota are present in large numbers and as a diverse population within the gastrointestinal tract. There are approximately 400 different species of microbiota which may be beneficial, harmful or both, but each play an important role in the regulation and modulation of the hosts’ bowel processes (McOrist et al. 2008; Dethlefsen et al. 2008). Many of these colon microbiota allow for saccharolytic fermentation of non-digestible dietary fibres and carbohydrates into by-products and intermediates, followed by a subsequent conversion into short chain fatty acids (SCFAs) (mainly n-butyric acid, propionic acid and acetic acid) each of which play an important role in maintaining colon homeostasis (Topping & Clifton 2001). A balance of ‘good’ microbiota (e.g., Bacteroides spp./ Bifidobacteria spp.) and ‘bad’ microbiota (e.g., Veilonellae) and the optimal production of various SCFAs within the gut could possibly allow for proper functioning of the large intestine and assist in decreasing the onset of various colonic disorders such as Irritable Bowel Syndrome (IBS). The sample group for the study consists of male and female patients, with an average age of 40 to 50 years old, whom of which have been diagnosed with either constipation IBS (C-IBS) or diarrhoea IBS (D-IBS) via the Rome III criteria system for IBS diagnosis. DNA and SCFA extractions were optimised for human stool, colonic fluid and tissue biopsy sample obtained from the aforementioned patients. Optimization steps allowed for starting material with high analysis integrity. Different methods of microbiota analysis, such as ARISA, were investigated; however, real-time qPCR was selected as the best method to identify and quantify specific microbiota. Extracted SCFAs were separated via gas chromatography and identified and quantified via Mass Spectrometry. Significant changes in microbial content and SCFA profiles were found to be associated with healthy and IBS patients. Results obtained would however be influenced by external factors typical of clinical studies of this nature. This study allows for opportunities for future research into understanding IBS.
- Full Text:
- Date Issued: 2013
High performance nonwovens in technical textile applications
- Ogunleye, Christopher Olarinde
- Authors: Ogunleye, Christopher Olarinde
- Date: 2013
- Subjects: Nonwoven fabrics , Textile fabrics -- Technological innovations
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10356 , http://hdl.handle.net/10948/d1021011
- Description: The aim of this research was to establish the optimum processing conditions and parameters for producing nonwoven fabrics best suited for application in disposable and protective wear for surgical gowns, drapes and laboratory coats. Carded and crosslapped webs, of three basic weights (80, 120, and 150g/m2), from greige (unscoured and unbleached) cotton, viscose and polyester fibres, were hydroentangled, using three different waterjet pressures (60, 100 and 120 bars), on a Fleissner Aquajet hydroentanglement machine. An antibacterial agent (Ruco-Coat FC 9005) and a fluorochemical water repellent agent (Ruco Bac-AGP), were applied in one bath using the pad-dry-cure technique, to impart both antibacterial and water repellent properties to the fabrics, SEM photomicrographs indicating that the finished polymers were evenly dispersed on the fabric surface. The effect of waterjet pressure, fabric weight and type and treatment on the structure of the nonwoven produced, was evaluated by measuring the relevant characteristics of the fabrics. As expected, there was an interrelationship between fabric weight, thickness, and density, the fabric thickness and mass density increasing with fabric weight. An increase in waterjet pressure decreased the fabric thickness and increased the fabric density. The water repellent and antibacterial treatment increased the fabric weight and thickness. The antimicrobial activity of the fabrics was assessed by determining the percentage reduction in Staphylococcus aureus and Escherichia coli bacteria population. The maximum percent reduction at 24hrs contact time for both bacteria ranged from 99.5 to 99.6 percent for all the fabric types. The standard spray test ratings for the three treated fabrics ranged from 80-90 percent, whereas that of the untreated water repellent fabric was zero, while the contact angles for all the fabric types exceeded 90 degrees, indicating good resistance to wetting. It was found that the tensile strength of the fabric in the cross-machine direction was higher than that in the machine direction, for both the treated and untreated fabrics, with the tensile strengths in both the MD and CD of the treated fabrics were greater than that of the untreated fabrics, the reverse being true for the extension at break. An increase in waterjet pressure increased the tensile strength but decreased the extension at break, for both the treated and untreated fabrics. The finishing treatment decreased the mean pore size of all the fabrics, the mean pore size decreasing with an increase in fabric weight and waterjet pressure. An increase in waterjet pressure and fabric weight decreased the air and water vapour permeability, as did the finishing treatment, although the differences were not always statistically significant. The polyester fabrics had the highest water and air permeability. Hence low weight fabrics of 80 g/m2, which were hydroentangled at low water jet pressures of 60 bars, were suitable for use in this study due to their higher air and water vapour permeability as well as higher pore size distribution. These group of fabrics thus meet the requirements for surgical gowns, drapes, nurses’ uniforms and laboratory coats.
- Full Text:
- Date Issued: 2013
- Authors: Ogunleye, Christopher Olarinde
- Date: 2013
- Subjects: Nonwoven fabrics , Textile fabrics -- Technological innovations
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10356 , http://hdl.handle.net/10948/d1021011
- Description: The aim of this research was to establish the optimum processing conditions and parameters for producing nonwoven fabrics best suited for application in disposable and protective wear for surgical gowns, drapes and laboratory coats. Carded and crosslapped webs, of three basic weights (80, 120, and 150g/m2), from greige (unscoured and unbleached) cotton, viscose and polyester fibres, were hydroentangled, using three different waterjet pressures (60, 100 and 120 bars), on a Fleissner Aquajet hydroentanglement machine. An antibacterial agent (Ruco-Coat FC 9005) and a fluorochemical water repellent agent (Ruco Bac-AGP), were applied in one bath using the pad-dry-cure technique, to impart both antibacterial and water repellent properties to the fabrics, SEM photomicrographs indicating that the finished polymers were evenly dispersed on the fabric surface. The effect of waterjet pressure, fabric weight and type and treatment on the structure of the nonwoven produced, was evaluated by measuring the relevant characteristics of the fabrics. As expected, there was an interrelationship between fabric weight, thickness, and density, the fabric thickness and mass density increasing with fabric weight. An increase in waterjet pressure decreased the fabric thickness and increased the fabric density. The water repellent and antibacterial treatment increased the fabric weight and thickness. The antimicrobial activity of the fabrics was assessed by determining the percentage reduction in Staphylococcus aureus and Escherichia coli bacteria population. The maximum percent reduction at 24hrs contact time for both bacteria ranged from 99.5 to 99.6 percent for all the fabric types. The standard spray test ratings for the three treated fabrics ranged from 80-90 percent, whereas that of the untreated water repellent fabric was zero, while the contact angles for all the fabric types exceeded 90 degrees, indicating good resistance to wetting. It was found that the tensile strength of the fabric in the cross-machine direction was higher than that in the machine direction, for both the treated and untreated fabrics, with the tensile strengths in both the MD and CD of the treated fabrics were greater than that of the untreated fabrics, the reverse being true for the extension at break. An increase in waterjet pressure increased the tensile strength but decreased the extension at break, for both the treated and untreated fabrics. The finishing treatment decreased the mean pore size of all the fabrics, the mean pore size decreasing with an increase in fabric weight and waterjet pressure. An increase in waterjet pressure and fabric weight decreased the air and water vapour permeability, as did the finishing treatment, although the differences were not always statistically significant. The polyester fabrics had the highest water and air permeability. Hence low weight fabrics of 80 g/m2, which were hydroentangled at low water jet pressures of 60 bars, were suitable for use in this study due to their higher air and water vapour permeability as well as higher pore size distribution. These group of fabrics thus meet the requirements for surgical gowns, drapes, nurses’ uniforms and laboratory coats.
- Full Text:
- Date Issued: 2013
Tolerance intervals for variance component models using a Bayesian simulation procedure
- Authors: Sarpong, Abeam Danso
- Date: 2013
- Subjects: Bayesian statistical decision theory , Multilevel models (Statistics)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10583 , http://hdl.handle.net/10948/d1021025
- Description: The estimation of variance components serves as an integral part of the evaluation of variation, and is of interest and required in a variety of applications (Hugo, 2012). Estimation of the among-group variance components is often desired for quantifying the variability and effectively understanding these measurements (Van Der Rijst, 2006). The methodology for determining Bayesian tolerance intervals for the one – way random effects model has originally been proposed by Wolfinger (1998) using both informative and non-informative prior distributions (Hugo, 2012). Wolfinger (1998) also provided relationships with frequentist methodologies. From a Bayesian point of view, it is important to investigate and compare the effect on coverage probabilities if negative variance components are either replaced by zero, or completely disregarded from the simulation process. This research presents a simulation-based approach for determining Bayesian tolerance intervals in variance component models when negative variance components are either replaced by zero, or completely disregarded from the simulation process. This approach handles different kinds of tolerance intervals in a straightforward fashion. It makes use of a computer-generated sample (Monte Carlo process) from the joint posterior distribution of the mean and variance parameters to construct a sample from other relevant posterior distributions. This research makes use of only non-informative Jeffreys‟ prior distributions and uses three Bayesian simulation methods. Comparative results of different tolerance intervals obtained using a method where negative variance components are either replaced by zero or completely disregarded from the simulation process, is investigated and discussed in this research.
- Full Text:
- Date Issued: 2013
- Authors: Sarpong, Abeam Danso
- Date: 2013
- Subjects: Bayesian statistical decision theory , Multilevel models (Statistics)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10583 , http://hdl.handle.net/10948/d1021025
- Description: The estimation of variance components serves as an integral part of the evaluation of variation, and is of interest and required in a variety of applications (Hugo, 2012). Estimation of the among-group variance components is often desired for quantifying the variability and effectively understanding these measurements (Van Der Rijst, 2006). The methodology for determining Bayesian tolerance intervals for the one – way random effects model has originally been proposed by Wolfinger (1998) using both informative and non-informative prior distributions (Hugo, 2012). Wolfinger (1998) also provided relationships with frequentist methodologies. From a Bayesian point of view, it is important to investigate and compare the effect on coverage probabilities if negative variance components are either replaced by zero, or completely disregarded from the simulation process. This research presents a simulation-based approach for determining Bayesian tolerance intervals in variance component models when negative variance components are either replaced by zero, or completely disregarded from the simulation process. This approach handles different kinds of tolerance intervals in a straightforward fashion. It makes use of a computer-generated sample (Monte Carlo process) from the joint posterior distribution of the mean and variance parameters to construct a sample from other relevant posterior distributions. This research makes use of only non-informative Jeffreys‟ prior distributions and uses three Bayesian simulation methods. Comparative results of different tolerance intervals obtained using a method where negative variance components are either replaced by zero or completely disregarded from the simulation process, is investigated and discussed in this research.
- Full Text:
- Date Issued: 2013
Single-end reflectometric measurements of polarization-mode dispersion in single-mode optical fibres
- Authors: Fosuhene, Samuel Kofi
- Date: 2013
- Subjects: Fiber optics , Polarization (Light) , Optical measurements
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/6280 , vital:21069
- Description: In this thesis two novel single-end methods are applied to measure and characterize polarization mode dispersion in single mode optical fibres. Polarization mode dispersion (PMD) is an important factor negatively affecting the successful implementation of high speed long haul optical fibre networks operating at bit rates of 10Gb/s and above. PMD measurements are thus important for quality control during manufacturing and cabling processes. It is also useful for network operators planning to upgrade bitrates in existing networks to 10Gb/s and beyond. In an optical fibre link, sections with particularly high PMD may act to increase the entire PMD of the link. Identifying and replacing such sections can greatly reduce the PMD of the link. PMD measurements can be forward or single-end. In forward measurements, both ends of the fibre are used for input and detection. In single-end configuration, only one end of the fibre is used. For this reason, single-end measurements are more practical for the field where fibre ends are situated several kilometres apart. Single-end techniques can be implemented with a continuous wave for non-local PMD measurements (by Fresnel reflection). If a pulsed wave is used, local measurements can be achieved (by total power due to Rayleigh scattering). Two single-end schemes, one based on Fresnel reflection and the other due to Rayleigh scattering have been applied to measure non-local and local PMD of standard single mode optical fibres. For the non-local PMD measurements, the general interferometric technique (GINTY) was modified to operate in a round-trip configuration. In this configuration, the fibre was treated as a concatenation of two identical fibre segments. Three different sets of fibres were investigated, each set representing a particular mode coupling regime. For polarization maintaining fibres, (PMFs), with no mode coupling, a factor of two was found between forward and single-end measurements. For long single mode fibres in the long length regime, the factor was 1.4. For a combination of PMF and single mode fibres, a factor of 1.6 was obtained. The method which is accurate, repeatable, low cost and robust is very suitable for field applications. The second method is the polarization optical time domain reflectometric (P-OTDR) technique. This technique performs local birefringence measurements by measuring the evolution of the states of polarization (SOP). The birefringence information from such measurements was extracted and analysed to characterise four different fibres. Beat lengths and correlation lengths extracted from the P-OTDR were used to calculate the differential group delay (DGD) of the fibres. Next an expression for the root-mean-square differential group delay was derived and applied to the birefringence measurements to calculate the DGDs at a single wavelength. This method which operates at a single wavelength has a huge advantage. Firstly it is able to measure completely all the fibre characteristic parameters. Secondly it can measure mean DGD, root mean square DGD and instantaneous DGD. A plot of instantaneous DGD vs. length enables one to identify and eliminate sections with particularly high DGD. Finally since the P-OTDR system operates with a single wavelength, real time monitoring of PMD is possible via multiplexing. The results obtained are repeatable, accurate and are in good agreement with the standard Jones Matrix Eigenanalysis (JME) technique.
- Full Text:
- Date Issued: 2013
Single-end reflectometric measurements of polarization-mode dispersion in single-mode optical fibres
- Authors: Fosuhene, Samuel Kofi
- Date: 2013
- Subjects: Fiber optics , Polarization (Light) , Optical measurements
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/6280 , vital:21069
- Description: In this thesis two novel single-end methods are applied to measure and characterize polarization mode dispersion in single mode optical fibres. Polarization mode dispersion (PMD) is an important factor negatively affecting the successful implementation of high speed long haul optical fibre networks operating at bit rates of 10Gb/s and above. PMD measurements are thus important for quality control during manufacturing and cabling processes. It is also useful for network operators planning to upgrade bitrates in existing networks to 10Gb/s and beyond. In an optical fibre link, sections with particularly high PMD may act to increase the entire PMD of the link. Identifying and replacing such sections can greatly reduce the PMD of the link. PMD measurements can be forward or single-end. In forward measurements, both ends of the fibre are used for input and detection. In single-end configuration, only one end of the fibre is used. For this reason, single-end measurements are more practical for the field where fibre ends are situated several kilometres apart. Single-end techniques can be implemented with a continuous wave for non-local PMD measurements (by Fresnel reflection). If a pulsed wave is used, local measurements can be achieved (by total power due to Rayleigh scattering). Two single-end schemes, one based on Fresnel reflection and the other due to Rayleigh scattering have been applied to measure non-local and local PMD of standard single mode optical fibres. For the non-local PMD measurements, the general interferometric technique (GINTY) was modified to operate in a round-trip configuration. In this configuration, the fibre was treated as a concatenation of two identical fibre segments. Three different sets of fibres were investigated, each set representing a particular mode coupling regime. For polarization maintaining fibres, (PMFs), with no mode coupling, a factor of two was found between forward and single-end measurements. For long single mode fibres in the long length regime, the factor was 1.4. For a combination of PMF and single mode fibres, a factor of 1.6 was obtained. The method which is accurate, repeatable, low cost and robust is very suitable for field applications. The second method is the polarization optical time domain reflectometric (P-OTDR) technique. This technique performs local birefringence measurements by measuring the evolution of the states of polarization (SOP). The birefringence information from such measurements was extracted and analysed to characterise four different fibres. Beat lengths and correlation lengths extracted from the P-OTDR were used to calculate the differential group delay (DGD) of the fibres. Next an expression for the root-mean-square differential group delay was derived and applied to the birefringence measurements to calculate the DGDs at a single wavelength. This method which operates at a single wavelength has a huge advantage. Firstly it is able to measure completely all the fibre characteristic parameters. Secondly it can measure mean DGD, root mean square DGD and instantaneous DGD. A plot of instantaneous DGD vs. length enables one to identify and eliminate sections with particularly high DGD. Finally since the P-OTDR system operates with a single wavelength, real time monitoring of PMD is possible via multiplexing. The results obtained are repeatable, accurate and are in good agreement with the standard Jones Matrix Eigenanalysis (JME) technique.
- Full Text:
- Date Issued: 2013
A model for supporting environmental awereness in higher education using social media
- Authors: Tlebere, Thabo Eugene
- Date: 2013
- Subjects: Social media , Environmental education , Universities and colleges
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:10496 , http://hdl.handle.net/10948/d1020820
- Description: University sustainability is a field of research that has been gaining increased interest in recent years. The reduction of environmental impact has become a strategic objective of universities globally. Universities have been prompted to take necessary action to ensure that their environmental impact is at a minimum. The environmental component of sustainability deals with the current conservation of the earth’s natural resources so that future generations can also have access to them. Human beings, due to their increasing needs, are accountable for the exploitation of natural resources. They are regarded as the main contributors to imbalances in the natural systems. Environmental concerns such as global warming, deforestations, disposal of wastes, and ozone reduction are the outcomes of the damage caused by humans on the environment. The aim of environmental education is to acquire remediation of the environment by making individuals aware of the environment and by educating them about how to live a more sustainable lifestyle. Environmental awareness is perceived as knowledge of the factors that affect the environment and having sensitivity towards the environment. Higher Education Institutions (HEIs) bear the responsibility of educating individuals about environmental issues since they provide education to future leaders in society who may have an influence on future conditions in the environment. Social media are capable of delivering information to a large spectrum of audiences at a low cost. The Pew Internet American Life Project reported that the number of adults who utilise social media has increased by 57 percent from 2005 to 2011. Several environmental activist organisations utilise social media to carry out environmental awareness campaigns. In this study two environmental awareness campaigns which were powered by social media were conducted to improve environmental awareness of individuals in a higher education environment. A Social media Model for ENvironmental Awareness (SMENA) was developed to facilitate the environmental awareness campaigns. The SMENA includes a website, social media as well as theoretical guidelines for creating environmental awareness campaigns, and for using social media for environmental awareness campaigns. A case study at the Nelson Mandela Metropolitan University (NMMU) was used to empirically evaluate SMENA. Students at the Department of Computer Sciences of NMMU were exposed to information about environmental issues through social media with the intention of improving their environmental knowledge and awareness. The SMENA website usability was rated positively and students enjoyed the blogs and information distributed by means of social media. The results of the study intervention were positive and showed that social media can be used to improve the environmental knowledge of students. This study provides a valuable contribution to both the field of environmental education and social media usage and acceptance. The guidelines and requirements for using social media to improve environmental awareness provided in this study can be used to assist educators and university management with addressing the problems of reducing environmental impact.
- Full Text:
- Date Issued: 2013
- Authors: Tlebere, Thabo Eugene
- Date: 2013
- Subjects: Social media , Environmental education , Universities and colleges
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:10496 , http://hdl.handle.net/10948/d1020820
- Description: University sustainability is a field of research that has been gaining increased interest in recent years. The reduction of environmental impact has become a strategic objective of universities globally. Universities have been prompted to take necessary action to ensure that their environmental impact is at a minimum. The environmental component of sustainability deals with the current conservation of the earth’s natural resources so that future generations can also have access to them. Human beings, due to their increasing needs, are accountable for the exploitation of natural resources. They are regarded as the main contributors to imbalances in the natural systems. Environmental concerns such as global warming, deforestations, disposal of wastes, and ozone reduction are the outcomes of the damage caused by humans on the environment. The aim of environmental education is to acquire remediation of the environment by making individuals aware of the environment and by educating them about how to live a more sustainable lifestyle. Environmental awareness is perceived as knowledge of the factors that affect the environment and having sensitivity towards the environment. Higher Education Institutions (HEIs) bear the responsibility of educating individuals about environmental issues since they provide education to future leaders in society who may have an influence on future conditions in the environment. Social media are capable of delivering information to a large spectrum of audiences at a low cost. The Pew Internet American Life Project reported that the number of adults who utilise social media has increased by 57 percent from 2005 to 2011. Several environmental activist organisations utilise social media to carry out environmental awareness campaigns. In this study two environmental awareness campaigns which were powered by social media were conducted to improve environmental awareness of individuals in a higher education environment. A Social media Model for ENvironmental Awareness (SMENA) was developed to facilitate the environmental awareness campaigns. The SMENA includes a website, social media as well as theoretical guidelines for creating environmental awareness campaigns, and for using social media for environmental awareness campaigns. A case study at the Nelson Mandela Metropolitan University (NMMU) was used to empirically evaluate SMENA. Students at the Department of Computer Sciences of NMMU were exposed to information about environmental issues through social media with the intention of improving their environmental knowledge and awareness. The SMENA website usability was rated positively and students enjoyed the blogs and information distributed by means of social media. The results of the study intervention were positive and showed that social media can be used to improve the environmental knowledge of students. This study provides a valuable contribution to both the field of environmental education and social media usage and acceptance. The guidelines and requirements for using social media to improve environmental awareness provided in this study can be used to assist educators and university management with addressing the problems of reducing environmental impact.
- Full Text:
- Date Issued: 2013
Foraging ecology of Cape Gannets (Morus Capensis) at Bird Island, Algoa Bay
- Authors: Green, David Bruce
- Date: 2013
- Subjects: Birds of prey -- Behavior -- South Africa -- Algoa Bay , Gannets -- South Africa -- Algoa Bay , Birds -- Breeding -- South Africa -- Algoa Bay
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10725 , http://hdl.handle.net/10948/d1020790
- Description: The Cape gannet has undergone considerable population change and redistribution over the past 50 years. This has been linked to shifts in the abundance and distribution of their dominant prey, sardine and anchovy. Five breeding colonies, along the west coast of Southern Africa, have shown rapid population declines as a result of reduced prey availability. In contrast, a single colony (Bird Island, Algoa Bay) on the south coast of South Africa has, over the same period, grown fivefold and now supports approximately two thirds of the total population. Due to its conservation importance, and isolation from other breeding localities, it is important to assess the health of the Bird Island colony, and determine how foraging distribution relates to the environment to evaluate current measures of protection. This was achieved through two related studies; a long-term dietary analysis spanning 34 years, and a spatial foraging study, which related three years of tracking data to estimates of prey availability, oceanographic features and marine protected areas (MPAs). The results of the dietary study showed that the dietary constituents of Cape gannets breeding at Bird Island have remained similar over the last three decades, but the importance of sardine and anchovy has increased significantly. For sardine, in particular, this reflects an increased availability of this species (as deduced from hydroacoustic surveys) within the foraging range of the Bird Island colony. The dietary abundance of anchovy was found to be negatively correlated with that of sardine. Surprisingly,.the dietary abundance of anchovy was also negatively correlated with estimates of its availability based on acoustic surveys. The latter is likely to be due to sardine being a preferred prey item. Recent decreases in the dietary contribution of sardine (since 2005) suggest that this species is becoming less available to gannets, with profound implications in terms of nutrient gain associated with foraging. However, this has been mediated by an increase in the dietary contribution of anchovy, which now accounts for the vast majority of prey taken. Spatially, the foraging range of the Bird Island colony expanded during the three years of study, indicating an increase in effort. This increase was likely in an effort to track a distributional change of sardine and anchovy, which showed an apparent westward shift during the study period. There was, however, no evidence of birds tracking features associated with high productivity. This may have been partly due to anomalously warm conditions during the summer of 2012/2013, in which an absence of coastal upwelling prevented surface cooling. Nonetheless, low sea surface temperatures and high chlorophyll a concentrations do not seem to be reliable indicators of important Cape gannet foragingareas. Foraging effort was largely concentrated outside of MPAs, indicating that the current MPA network provides little protection for foraging gannets. This could change with the additional protection of the proposed Greater Addo Elephant National Park MPA expansion, as prey species are allowed to recover following the removal of fishing pressure. Overall, the colony appears to be in good condition as the diet is still dominated by live prey items, and the foraging range remains smaller than many of the colonies along the west coast. However, it is important that monitoring be continued, in particular with respect to changes in the availability of sardine and anchovy. Long-term shifts of these species out of the colony‘s foraging range could negatively influence the population in the future. This might be worsened by interspecific competition for prey resources. Considering the conservation importance of this population, maintenance of healthy prey stocks within the home range of breeding Cape gannets should be prioritised in order to prevent declines similar to those observed at west coast colonies.
- Full Text:
- Date Issued: 2013
- Authors: Green, David Bruce
- Date: 2013
- Subjects: Birds of prey -- Behavior -- South Africa -- Algoa Bay , Gannets -- South Africa -- Algoa Bay , Birds -- Breeding -- South Africa -- Algoa Bay
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10725 , http://hdl.handle.net/10948/d1020790
- Description: The Cape gannet has undergone considerable population change and redistribution over the past 50 years. This has been linked to shifts in the abundance and distribution of their dominant prey, sardine and anchovy. Five breeding colonies, along the west coast of Southern Africa, have shown rapid population declines as a result of reduced prey availability. In contrast, a single colony (Bird Island, Algoa Bay) on the south coast of South Africa has, over the same period, grown fivefold and now supports approximately two thirds of the total population. Due to its conservation importance, and isolation from other breeding localities, it is important to assess the health of the Bird Island colony, and determine how foraging distribution relates to the environment to evaluate current measures of protection. This was achieved through two related studies; a long-term dietary analysis spanning 34 years, and a spatial foraging study, which related three years of tracking data to estimates of prey availability, oceanographic features and marine protected areas (MPAs). The results of the dietary study showed that the dietary constituents of Cape gannets breeding at Bird Island have remained similar over the last three decades, but the importance of sardine and anchovy has increased significantly. For sardine, in particular, this reflects an increased availability of this species (as deduced from hydroacoustic surveys) within the foraging range of the Bird Island colony. The dietary abundance of anchovy was found to be negatively correlated with that of sardine. Surprisingly,.the dietary abundance of anchovy was also negatively correlated with estimates of its availability based on acoustic surveys. The latter is likely to be due to sardine being a preferred prey item. Recent decreases in the dietary contribution of sardine (since 2005) suggest that this species is becoming less available to gannets, with profound implications in terms of nutrient gain associated with foraging. However, this has been mediated by an increase in the dietary contribution of anchovy, which now accounts for the vast majority of prey taken. Spatially, the foraging range of the Bird Island colony expanded during the three years of study, indicating an increase in effort. This increase was likely in an effort to track a distributional change of sardine and anchovy, which showed an apparent westward shift during the study period. There was, however, no evidence of birds tracking features associated with high productivity. This may have been partly due to anomalously warm conditions during the summer of 2012/2013, in which an absence of coastal upwelling prevented surface cooling. Nonetheless, low sea surface temperatures and high chlorophyll a concentrations do not seem to be reliable indicators of important Cape gannet foragingareas. Foraging effort was largely concentrated outside of MPAs, indicating that the current MPA network provides little protection for foraging gannets. This could change with the additional protection of the proposed Greater Addo Elephant National Park MPA expansion, as prey species are allowed to recover following the removal of fishing pressure. Overall, the colony appears to be in good condition as the diet is still dominated by live prey items, and the foraging range remains smaller than many of the colonies along the west coast. However, it is important that monitoring be continued, in particular with respect to changes in the availability of sardine and anchovy. Long-term shifts of these species out of the colony‘s foraging range could negatively influence the population in the future. This might be worsened by interspecific competition for prey resources. Considering the conservation importance of this population, maintenance of healthy prey stocks within the home range of breeding Cape gannets should be prioritised in order to prevent declines similar to those observed at west coast colonies.
- Full Text:
- Date Issued: 2013
Production of recombinant human CD21 and CD23 : towards a better understanding of their interaction
- Authors: Van Zyl, Dwain George
- Date: 2013
- Subjects: Immunoglobulins , Fc receptors
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10365 , http://hdl.handle.net/10948/d10211135
- Description: The prevalence of allergic diseases has dramatically increased over the last three decades. Presently, it is estimated that 20-30 per cent of the developed world suffers from allergic diseases. The majority of allergic diseases are rooted in the activities of IgE; an immunoglobulin which exerts its effector functions by interacting with a network of proteins. This network includes its low affinity receptor CD23. Cross linking of membrane IgE and CD21 by soluble CD23 results in an increase in IgE synthesis. This marks the interaction between CD23 and CD21 as an attractive therapeutic target. However, details regarding this interaction are inadequate for rational drug design. To obtain a deeper understanding of the CD23-CD21 interaction recombinant human CD21 (SCR1-2 and SCR5-8) and CD23 (16 kD and 25 kDa) were produced. The cloning, expression and purification of recombinant proteins comprised a significant portion of this study. Recombinant CD23 was expressed as inclusion bodies, refolded by rapid dilution and purified by size exclusion chromatography. Conversely, recombinant CD21 was expressed as soluble MBP-fusions and purified with an amylose affinity resin. The interaction between recombinant CD23 and CD21 was analysed by flow cytometry and ELISA experiments. Flow cytometry showed that 16 kDa and 25 kDa CD23 interacted with SCR5-8 to the same extent. Semi-quantitative ELISA experiments showed that both SCR1-2 and SCR5-8 were able to interact with 16 kDa and 25 kDa CD23. This suggests that the binding sites of SCR1-2 and SCR5-8 occur on 16 kDa CD23. Furthermore, since proteins were expressed in E. coli it suggests that the CD23-CD21 interaction does not require glycosylation. Furthermore, considering what is known about the SCR1-2-CD23 interaction from previous NMR studies; i.e. that the C-terminal tail (residues residues 289-298) of CD23 is responsible for binding SCR1-2, indicates that SCR5-8 binds somewhere within the lectin domain of CD23. This indicates that the CD23-CD21 interaction involves C-terminal tail-SCR1-2 and lectin domain-SCR5-8 interactions.
- Full Text:
- Date Issued: 2013
- Authors: Van Zyl, Dwain George
- Date: 2013
- Subjects: Immunoglobulins , Fc receptors
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10365 , http://hdl.handle.net/10948/d10211135
- Description: The prevalence of allergic diseases has dramatically increased over the last three decades. Presently, it is estimated that 20-30 per cent of the developed world suffers from allergic diseases. The majority of allergic diseases are rooted in the activities of IgE; an immunoglobulin which exerts its effector functions by interacting with a network of proteins. This network includes its low affinity receptor CD23. Cross linking of membrane IgE and CD21 by soluble CD23 results in an increase in IgE synthesis. This marks the interaction between CD23 and CD21 as an attractive therapeutic target. However, details regarding this interaction are inadequate for rational drug design. To obtain a deeper understanding of the CD23-CD21 interaction recombinant human CD21 (SCR1-2 and SCR5-8) and CD23 (16 kD and 25 kDa) were produced. The cloning, expression and purification of recombinant proteins comprised a significant portion of this study. Recombinant CD23 was expressed as inclusion bodies, refolded by rapid dilution and purified by size exclusion chromatography. Conversely, recombinant CD21 was expressed as soluble MBP-fusions and purified with an amylose affinity resin. The interaction between recombinant CD23 and CD21 was analysed by flow cytometry and ELISA experiments. Flow cytometry showed that 16 kDa and 25 kDa CD23 interacted with SCR5-8 to the same extent. Semi-quantitative ELISA experiments showed that both SCR1-2 and SCR5-8 were able to interact with 16 kDa and 25 kDa CD23. This suggests that the binding sites of SCR1-2 and SCR5-8 occur on 16 kDa CD23. Furthermore, since proteins were expressed in E. coli it suggests that the CD23-CD21 interaction does not require glycosylation. Furthermore, considering what is known about the SCR1-2-CD23 interaction from previous NMR studies; i.e. that the C-terminal tail (residues residues 289-298) of CD23 is responsible for binding SCR1-2, indicates that SCR5-8 binds somewhere within the lectin domain of CD23. This indicates that the CD23-CD21 interaction involves C-terminal tail-SCR1-2 and lectin domain-SCR5-8 interactions.
- Full Text:
- Date Issued: 2013
Evaluation of eucalyptus citriodora derived p-menthane-3,8-diol-citronellal acetal as a bio-plasticizer for cosmetic application
- Authors: Burger, Kirstin
- Date: 2013
- Subjects: Plasticizers , Eucalyptus citriodora
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10420 , http://hdl.handle.net/10948/d1014080
- Description: Plasticizers are generally added to cosmetic and personal care products to improve the filmforming abilities of the product and increase flexibility of the film formed on the skin or hair surface. For example, plasticizers are present in perfumes to prolong the release of the specific scent, which is the ultimate goal in a good quality perfume. Plasticizers in nail varnishes prevent chipping, improve the aesthetics by adhering to the keratin in the nail which means the coating stays on for much longer, which is the ultimate goal in nail products. Plasticizers improve the gloss, resist chipping and allow quick drying time. Therefore it can be seen that plasticizers play a vital role in personal care products like perfumes and nail varnishes. Certain plasticizers e.g. phthalates, can cause problems associated with human health and can harm the environment. They are easily available and large volumes can be obtained at a low cost. These phthalates, for example, di-butyl phthalate (DBP) have been identified as carcinogenic. Nowadays the occurrence of cancer is rapidly increasing. The plasticizers present in a large number of consumer and personal care products, can possibly be linked to the ever increasing reports of cancer. Therefore a substitute to the traditional phthalate plasticizers must be investigated. The aim of this research is to produce a plasticizer derived from naturally occurring Eucalyptus oil, which can be used to replace the existing plasticizers in cosmetic formulations. Para-menthane-3,8-diol (PMD), occurring naturally in the oil from the tree, Eucalyptus citriodora, forms an acetal with citronellal (PMD, acetal, citronellal all occur naturally in the oil). It has been previously shown that PMD-citronellal acetal will exhibit plasticizing properties similar to conventional plasticizers. The objective was to enhance the formation of the acetal in the Eucalyptus oil by reacting it with excess PMD. An effective synthesis method for the PMD-citronellal acetal enriched oil (~73.8 percent) was determined from optimization experiments. The physical characterisation of the PMD-citronellal acetal enriched oil was done and compared with that of DBP. The acetal-enriched oil had a lower density, slightly higher solubility in water (at 25°C), lower refractive index (Brix percent) and a higher boiling point (350°C) than DBP. The physical characteristics of the Eucalyptus oil source and the acetal-enriched Eucalyptus oil were very similar. This can be expected as the Eucalyptus oil consists of ~84.3 percent Citronellal, ~ 1.3 percent PMD and 2.7 percent PMD-citronellal acetal. In this study the effectiveness of the acetal-enriched Eucalyptus oil (referred to from now on as the bio-plasticizer) was compared to a conventional plasticizer such as di-butyl phthalate (DBP), commonly used in cosmetic products. Two cosmetic formulations were produced: a nail varnish and a perfume formulation. Various tests were performed on these formulations to investigate the plasticizing properties of the bio-plasticizer. The objectives were to determine if the natural plasticizer is as effective as the potentially carcinogenic phthalate plasticizers and can be used as a substitute for the phthalates in personal care products. The results indicate that the bio-plasticizer does behave similarly to di-butyl phthalate, however, the effectiveness of the bio-plasticizer is lower than that of di-butyl phthalate. As the viscosity of the synthesized oil was high, this affected the overall consistency of the products. A more viscous nail varnish and perfume was produced in comparison to the DBP counterpart. The stability of the bio-plasticizer in the cosmetic formulations of nail varnish and perfume was also investigated. The cosmetic products were incubated at 0°C, 25°C and 40°C over a period of two months. Any changes in colour, odour, pH, refractive index, separation and plasticizer peak change in the gas chromatogram trace were recorded. It was determined that the PMD-citronellal acetal-enriched oil was relatively unstable under elevated temperatures and light intensity. Storage under higher temperatures (40°C) tends to increase the acidity. Therefore the bio-plasticizer must be placed in a closed, covered bottle and stored in an environment away from light and elevated temperatures. According to the gas chromatogram peaks, it was clear that both the bio-plasticizer and the DBP were more unstable in the perfume formulation than in the nail polish and were especially sensitive to light when in the perfume. This could possibly be due to the interaction with the fragrance molecule, p-anisaldehyde.
- Full Text:
- Date Issued: 2013
- Authors: Burger, Kirstin
- Date: 2013
- Subjects: Plasticizers , Eucalyptus citriodora
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10420 , http://hdl.handle.net/10948/d1014080
- Description: Plasticizers are generally added to cosmetic and personal care products to improve the filmforming abilities of the product and increase flexibility of the film formed on the skin or hair surface. For example, plasticizers are present in perfumes to prolong the release of the specific scent, which is the ultimate goal in a good quality perfume. Plasticizers in nail varnishes prevent chipping, improve the aesthetics by adhering to the keratin in the nail which means the coating stays on for much longer, which is the ultimate goal in nail products. Plasticizers improve the gloss, resist chipping and allow quick drying time. Therefore it can be seen that plasticizers play a vital role in personal care products like perfumes and nail varnishes. Certain plasticizers e.g. phthalates, can cause problems associated with human health and can harm the environment. They are easily available and large volumes can be obtained at a low cost. These phthalates, for example, di-butyl phthalate (DBP) have been identified as carcinogenic. Nowadays the occurrence of cancer is rapidly increasing. The plasticizers present in a large number of consumer and personal care products, can possibly be linked to the ever increasing reports of cancer. Therefore a substitute to the traditional phthalate plasticizers must be investigated. The aim of this research is to produce a plasticizer derived from naturally occurring Eucalyptus oil, which can be used to replace the existing plasticizers in cosmetic formulations. Para-menthane-3,8-diol (PMD), occurring naturally in the oil from the tree, Eucalyptus citriodora, forms an acetal with citronellal (PMD, acetal, citronellal all occur naturally in the oil). It has been previously shown that PMD-citronellal acetal will exhibit plasticizing properties similar to conventional plasticizers. The objective was to enhance the formation of the acetal in the Eucalyptus oil by reacting it with excess PMD. An effective synthesis method for the PMD-citronellal acetal enriched oil (~73.8 percent) was determined from optimization experiments. The physical characterisation of the PMD-citronellal acetal enriched oil was done and compared with that of DBP. The acetal-enriched oil had a lower density, slightly higher solubility in water (at 25°C), lower refractive index (Brix percent) and a higher boiling point (350°C) than DBP. The physical characteristics of the Eucalyptus oil source and the acetal-enriched Eucalyptus oil were very similar. This can be expected as the Eucalyptus oil consists of ~84.3 percent Citronellal, ~ 1.3 percent PMD and 2.7 percent PMD-citronellal acetal. In this study the effectiveness of the acetal-enriched Eucalyptus oil (referred to from now on as the bio-plasticizer) was compared to a conventional plasticizer such as di-butyl phthalate (DBP), commonly used in cosmetic products. Two cosmetic formulations were produced: a nail varnish and a perfume formulation. Various tests were performed on these formulations to investigate the plasticizing properties of the bio-plasticizer. The objectives were to determine if the natural plasticizer is as effective as the potentially carcinogenic phthalate plasticizers and can be used as a substitute for the phthalates in personal care products. The results indicate that the bio-plasticizer does behave similarly to di-butyl phthalate, however, the effectiveness of the bio-plasticizer is lower than that of di-butyl phthalate. As the viscosity of the synthesized oil was high, this affected the overall consistency of the products. A more viscous nail varnish and perfume was produced in comparison to the DBP counterpart. The stability of the bio-plasticizer in the cosmetic formulations of nail varnish and perfume was also investigated. The cosmetic products were incubated at 0°C, 25°C and 40°C over a period of two months. Any changes in colour, odour, pH, refractive index, separation and plasticizer peak change in the gas chromatogram trace were recorded. It was determined that the PMD-citronellal acetal-enriched oil was relatively unstable under elevated temperatures and light intensity. Storage under higher temperatures (40°C) tends to increase the acidity. Therefore the bio-plasticizer must be placed in a closed, covered bottle and stored in an environment away from light and elevated temperatures. According to the gas chromatogram peaks, it was clear that both the bio-plasticizer and the DBP were more unstable in the perfume formulation than in the nail polish and were especially sensitive to light when in the perfume. This could possibly be due to the interaction with the fragrance molecule, p-anisaldehyde.
- Full Text:
- Date Issued: 2013